scholarly journals Changes in osmoregulatory metabolism of cotton genotypes during water deficit and recovery period

2019 ◽  
Vol 23 (8) ◽  
pp. 607-613
Author(s):  
Érika C. F. de Macêdo ◽  
João H. Zonta ◽  
Yuri L. Melo ◽  
Alberto S. de Melo ◽  
Duval C. da Silva ◽  
...  
Keyword(s):  
Water Deficit ◽  
Stress Condition ◽  
Water Status ◽  
Recovery Period ◽  
Compatible Solutes ◽  
Randomized Design ◽  
Cotton Genotypes ◽  
Completely Randomized Design ◽  
Sensitive Genotype ◽  
Growth Indicators

ABSTRACT The aim of this study was to evaluate the changes in osmoregulatory metabolism of cotton genotypes subjected to water deficit and recovery period. In a greenhouse, six cotton genotypes and two water managements were combined in a 6 x 2 factorial scheme, in a completely randomized design, evaluated after 14 and 22 days under water stress and recovery conditions, respectively. The water status, growth indicators and compatible solutes in the leaves were evaluated. The genotypes BRS Seridó, BRS Aroeira and BRS 7MH withstand better the stress condition because they had lower variations in their water potentials due to the osmotic adjustment and maintained their growth under water deficit conditions. The BRS 286 is the most sensitive genotype to the water deficit condition, since it showed greater variations in its water potential, which ultimately stopped its growth. Water replacement during the recovery period allowed growth resumption in most of the genotypes, mainly CNPA 5M and BRS 286. After the recovery period, variations in the concentrations of osmoregulators indicate the plasticity of cotton in regulating the concentrations of osmoregulators under favorable and unfavorable water conditions.

scholarly journals Chlorophyll a fluorescence as an indicator of water stress in Calophyllum brasiliense

2020 ◽  
Vol 48 (1) ◽  
pp. 210-220 ◽  
Author(s):  
Lucas C. REIS ◽  
Silvana P.Q. SCALON ◽  
Daiane DRESCH ◽  
Andressa Caroline FORESTI ◽  
Cleberton C. SANTOS ◽  
...  
Keyword(s):  
Water Deficit ◽  
Chlorophyll A ◽  
Chlorophyll A Fluorescence ◽  
Stress Condition ◽  
Water Status ◽  
Photosynthetic Apparatus ◽  
Stress Indicator ◽  
Fluorescence Measurements ◽  
Calophyllum Brasiliense ◽  
And Function

The objective of this study was to evaluate chlorophyll a fluorescence as a stress indicator in Calophyllum brasiliense Cambess seedlings grown with different concentrations of abscisic acid (ABA) under intermittent water deficit condition: daily irrigation without ABA (I); daily irrigation + 10 μM ABA (I 10); daily irrigation + 100 μM ABA (I 100); suspension of daily irrigation without ABA (SI); suspension of daily irrigation + 10 μM ABA (SI 10) and  suspension of daily irrigation + 100 μM ABA (SI 100). The intermittent water deficit reduces water status and impairs the photochemical apparatus functioning and seedling quality. The fluorescence measurements helped identify the stress condition of water deficit in the cultivation of C. brasiliense and the beneficial effect of the application of 10 μM of ABA in minimizing stress and facilitating the recovery of seedlings after re-irrigation, while maintaining the integrity and function of the photosynthetic apparatus.

scholarly journals Seleksi Mutan Tanaman Lamtoro (Leucaena leucocephala cv. Tarramba) Tahan Kutu Loncat terhadap Lingkungan Kering pada Rumah Kaca

2021 ◽  
Vol 19 (3) ◽  
pp. 90-94
Author(s):  
Rini Anggriani ◽  
P D M H Karti ◽  
I Prihantoro
Keyword(s):  
Gamma Rays ◽  
Leucaena Leucocephala ◽  
Stress Condition ◽  
Acid Stress ◽  
Stem Diameter ◽  
Randomized Design ◽  
Drought Resistant ◽  
Completely Randomized Design ◽  
Different Sources ◽  
Different Levels

Forage is the main source of feed for ruminants. Forage consists of two types, namely grass and legumes. Leguminosa is a type of forage as a source of protein. One type of legume that is well known by breeders in Indonesia is lamtoro (Leucaena leucocephala cv. Tarramba). This study was aimed to produce candidate mutants of lamtoro drought resistant to acid stress conditions. The experiment in this study used an unbalanced completely randomized design with 6 treatments of 740 different replications based on different sources of lamtoro plant mutants at different levels (P0: 0 n= 89, P1: 100 gy n= 82, P2: 200 gy n= 153, P3:300 gy n=120, P4: 400 gy n= 244, P5: 500 gy n= 52). Variables observed included plant height, number of stalks, leaf loss and stem diameter. The results showed that lamtoro plants irradiated with gamma rays at a level of 200 gy-500 gy were significantly higher than 100 gy irradiation at 10 and 12 days watering, but the diameter of the plants was larger at 100 gy irradiation. It can be watering intervals of 10 days and 12 days on the parameters of height and stem diameter showed that gamma rays irradiation of 400 gy resulted in dry-resistant lamtoro mutant candidates under acid stress condition. Key words:        gamma rays, lamtoro mutant,  watering interval

scholarly journals POTENCIAL HÍDRICO FOLIAR EM MILHO SUBMETIDO AO DÉFICIT HÍDRICO

Irriga ◽  
2010 ◽  
Vol 15 (3) ◽  
pp. 324-334
Author(s):  
Juliano Dalcin Martins ◽  
Reimar Carlesso ◽  
Alberto Eduardo Knies ◽  
Zanandra Boff de Oliveira ◽  
Tiago Broetto ◽  
...  
Keyword(s):  
Water Potential ◽  
Water Deficit ◽  
Leaf Water Potential ◽  
Irrigation Management ◽  
Leaf Water ◽  
Maize Leaf ◽  
Randomized Design ◽  
Development Cycle ◽  
Rainout Shelter ◽  
Completely Randomized Design

O potencial hídrico foliar pode ser considerado como um parâmetro de indicação da condição hídrica das culturas. Este trabalho teve como objetivo estudar o potencial hídrico foliar das plantas de milho sobre déficit hídrico no estágio inicial de desenvolvimento reprodutivo. O experimento foi conduzido no interior de uma cobertura móvel “Rainout shelter”, utilizando o delineamento experimental inteiramente casualizado, com dois tratamentos e quinze repetições. Os tratamentos foram constituídos de dois manejos da água de irrigação: (i) irrigado (as plantas de milho receberam irrigação conforme suas necessidades durante todo o ciclo de desenvolvimento); (ii) déficit hídrico aplicado antes do pendoamento.   O déficit hídrico teve uma duração de aproximadamente 50 mm de ETo acumulada. A cobertura móvel foi acionada quando da ocorrência de chuvas, impedindo que esta ocorresse sobre a área experimental. Determinou-se o conteúdo de água disponível nas camadas de 0-10, 10-25, 25-55 e 55-85 cm de profundidade e variação diária do potencial hídrico das folhas de milho. Não foram observadas diferenças entre os tratamentos para o potencial de água na folha. O potencial hídrico foliar em plantas de milho não apresenta variação para déficit hídrico de 50 mm de evapotranspiração de referência acumulada.   UNITERMOS: déficit hídrico, potencial hídrico foliar, milho, irrigação.     MARTINS, J. D.; CARLESSO. R.; KNIES, A. E.; OLIVEIRA, Z. B.; BROETTO, T.; RODRIGUES, G. J. LEAF WATER POTENTIAL IN MAIZE EXPOSED TO WATER DEFICIT 2 ABSTRACT   The leaf water potential can be considered as a parameter for indicating the crops water condition. This work aimed to study the maize leaf water potential on water deficit in the reproductive developmentinitial stage. The experiment was conducted within a mobile coverage "rainout shelter" using a completely randomized design with two treatments and fifteen repetitions. The treatments consisted of two water irrigation management: (i) irrigated (maize was irrigated as needed throughout the development cycle), (ii) water stress applied before tasseling. The drought lasted approximately 50 mm of accumulated reference evapotranspiration (ETo). The mobile coverage was triggered when rainfall occurred, preventing water from falling into the experimental area. Available water was determined  in layers of 0-10, 10-25, 25-55 and 55-85 cm depth and daily variation of water potential in maize leaves were also  evaluated. There were no differences between treatments for leaf water potential. The maize leaf water potential presented no change to water deficit equivalent to 50 mm of accumulated ETo.  KEYWORDS: water deficit, leaf water potential, maize, irrigation.

scholarly journals Defisit Air Pada Berbagai Fase Pertumbuhan dan Pengaruhnya Terhadap Karakter Kuantitatif Beberapa Genotipe Kacang Tanah

2021 ◽  
pp. 44-54
Author(s):  
A Farid Hemon ◽  
Sumarjan Sumarjan ◽  
Baiq Erna Listiana ◽  
Suprayanti Martia Dewi
Keyword(s):  
Plant Growth ◽  
Water Deficit ◽  
Vegetative Phase ◽  
Seed Filling ◽  
Quantitative Characters ◽  
Randomized Design ◽  
Filling Phase ◽  
Completely Randomized Design ◽  
Generative Phase ◽  
Plot Design

This study aims to determine the effect of water deficit at various phases of plant growth on the quantitative characters of several peanut genotypes. This study used a completely randomized design-split plot design.  The water deficit consisted of 6 treatments: d0 = no water deficit, d1 = water deficit from germination to harvest, d2 = water deficit from germination to age 25 days after planting (dap) (vegetative phase), d3 = water deficit from age 26  to 50 dap (flowering phase to pod formation), d4 = water deficit from age 51 dap to 75 dap (seed filling phase), and d5 = water deficit from age 75 dap to 100 dap (seed ripening phase until harvest). The peanut genotype used consisted of 10 genotypes. The results showed that water deficit in various phases of plant growth resulted in different quantitative characters in several peanut genotypes. Genotype G3T4 produced heaviest dry pod weight of 12.7 g plant-1 in water deficit from germination to harvest. Genotype G200-I produced heaviest dry pod weight of 11.5 g per plant-1 in water deficit in the vegetative phase. Genotype G3T4 produced heaviest dry pod weight of 13.3 g per plant in water deficit the generative phase. Genotype G300-II produced heaviest the dry pod weight of 11.7 g per plant-1 in the water deficit of the seed filling phase. Genotypes G2D2, G2T3 and G200-I produced the heaviest dry pod weight of 11.0 g per plant-1 in the water deficit of the seed ripening phase.

scholarly journals IDENTIFICAÇÃO DE GENÓTIPOS DE FEIJÃO-CAUPI TOLERANTES E SUSCETÍVEIS AO DÉFICIT HÍDRICO

Nativa ◽  
2018 ◽  
Vol 6 (6) ◽  
pp. 608
Author(s):  
Eveline Nogueira Lima ◽  
Kátia Regina Alves Pires ◽  
Elaine Facco Celin ◽  
Cândida Hermínia Campos de Magalhães Bertini ◽  
Rosilene Oliveira Mesquita
Keyword(s):  
Polyethylene Glycol ◽  
Water Stress ◽  
Water Deficit ◽  
Vigna Unguiculata ◽  
The Other ◽  
Distilled Water ◽  
Water Deficiency ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Selection Of

Objetivou-se com esse trabalho identificar genótipos de feijão-caupi tolerantes e suscetíveis ao déficit hídrico, utilizando-se duas metodologias, estresse hídrico simulado com o uso de PEG6000 (Polietilenoglicol) e Screening Box. Os experimentos foram conduzidos em casa de vegetação no delineamento inteiramente casualizado com três repetições. Para a metodologia com PEG6000 foram utilizados 15 genótipos e duas testemunhas, com três repetições, totalizando 90 amostras. Foram selecionadas seis plântulas por genótipo, utilizaram-se três caixas contendo apenas água destilada e outras três com nível de potencial osmótico de - 0,2 MPa de PEG6000. A avaliação foi feita até atingir o ponto máximo de crescimento das raízes. Para a metodologia de Screening Box foram utilizados 15 genótipos, onde estes foram avaliados quanto à tolerância ao déficit hídrico no estádio de plântula. As duas metodologias mostraram-se eficientes na seleção de genótipos de feijão-caupi, os genótipos Santo Inácio Vermelho e BRS Tumucumaque, foram os mais sensíveis à seca, em ambas as metodologias.Palavras-chave: Vigna unguiculata, seca, plântulas. COWPEA GENOTYPES TOLERANT AND SUSCEPTIBLE TO WATER DEFICIT ABSTRACT: Objective to identify tolerant and susceptible cowpeas genotypes to water deficiency at the seedlings stage of growth, using two methodologies such as (a) water stress with the use of PEG6000 (Polyethylene glycol) and the Screening Box were used in the present research. The experiments were conducted in greenhouse environments using a completely randomized design with three replications. For the PEG6000 approach, 15 genotypes and two controls treatments were used in three replications in a total of 90 samples. Six seedlings were selected per genotype by using three boxes containing only distilled water. In the other treatment, three level of osmotic potential of -0.2 MPa of PEG6000. The evaluations of the trials were done until the Screening Box approach, 15 genotypes were used and were evaluated for tolerance to water deficit at the seedling stage of growth. The two methodologies were efficient in the selection of cowpea genotypes, the genotypes Santo InácioVermelho and BRS Tumucumaque were the most sensitive to drought in both methodologies.Keywords: drought, Vigna unguiculata, seedlings.

scholarly journals AKTIVITAS FISIK MAKSIMAL AKUT (ACUTE OVERTRAINING) MENYEBABKAN KERUSAKAN SEL β PANKREAS MENCIT

2015 ◽  
Vol 7 (2) ◽  
Author(s):  
Ferbian M. Siswanto ◽  
Boedi P. Yenniastuti ◽  
Tri A. Putra ◽  
I Made Kardena
Keyword(s):  
Oxidative Stress ◽  
Recovery Period ◽  
The Body ◽  
Physical Work ◽  
Type I ◽  
Β Cells ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design ◽  
One Way Anova

Abstract: Excessive physical activity can cause oxidative stress characterized by increased free radicals in the body. This oxidative stress will cause damages to a variety of cells, inter alia β cells of islets of Langerhans in pancreas. This study aimed to determine that acute overtraining could increase the risk of type I diabetes mellitus through induction of pancreatic β cell damages. This study used a completely randomized design with total samples of 36 mice divided into 3 groups. Group P0 was the group of mice without treatment; group P1 was treated with overworking and a recovery period of 48 hours; and group P2 was treated with overworking and a recovery period of 24 hours. After 14 days of treatment, the pancreas of all groups were taken for histological examination using Gomori chrome hematoxylin phloxine B staining. The descriptive analysis showed that the pancreatic tissues of groups P1 and P2 were morphologically damaged compared to group P0. The results of quantitative observations were analyzed by using One way Anova test followed by LSD, indicated that the number of pancreatic β-cells was significantly decreased among the groups (P < 0.05). Conclusion: Acute overtraining may cause damages of pancreatic β cells.Keywords: excessive physical work, pancreatic β cellsAbstrak: Aktivitas fisik berlebihan dapat menyebabkan terjadinya stres oksidatif yang ditandai dengan meningkatnya radikal bebas di dalam tubuh. Stres oksidatif akan menyebabkan kerusakan berbagai macam sel, salah satunya sel β pulau langerhans pankreas. Penelitian ini bertujuan untuk mengetahui aktivitas fisik maksimal akut (acute overtraining) dalam meningkatkan risiko terjadinya diabetes melitus tipe I melalui induksi kerusakan sel β pankreas. Rancangan penelitian ini ialah completely randomized design dengan sampel 36 ekor mencit yang dibagi menjadi 3 kelompok. Kelompok P0 ialah kelompok mencit tanpa perlakuan; kelompok P1 diberi perlakuan beban kerja maksimal dengan periode pemulihan selama 48 jam; dan kelompok P2 diberi perlakuan beban kerja maksimal dengan periode pemulihan selama 24 jam. Setelah 14 hari perlakuan, keseluruhan kelompok sampel diambil organ pankreasnya untuk dibuat sediaan histologik dengan pewarnaan khusus Gomori chrome hematoxylin phloxine B. Analisis deskriptif kualitatif menunjukkan bahwa morfologi jaringan pankreas pada kelompok P1 dan P2 mengalami kerusakan jika dibandingkan P0. Hasil pengamatan kuantitatif dianalisis dengan menggunakan uji One way Anova dilanjutkan dengan Least Significant Difference (LSD), menunjukkan bahwa terjadi penurunan jumlah sel β pankreas yang signifikan antar kelompok P0, P1, dan P2 (P < 0,05). Simpulan: Aktivitas fisik maksimal akut dapat menyebabkan kerusakan sel β pankreas.Kata kunci: beban kerja maksimal, sel β pankreas

scholarly journals Effect of Water Deficit Stress and Foliar Application of Salicylic Acid on Antioxidants Enzymes Activity in Leaves of Thymus Daenensis Subsp. Lancifolius

2015 ◽  
Vol 48 (1) ◽  
pp. 57-67 ◽  
Author(s):  
A.A. Bahari ◽  
R. Sokhtesaraei ◽  
H.R. Chaghazardi ◽  
F. Masoudi ◽  
H. Nazarli
Keyword(s):  
Salicylic Acid ◽  
Water Deficit ◽  
Foliar Application ◽  
Water Deficit Stress ◽  
Oxygen Species ◽  
Antioxidants Enzymes ◽  
Signal Cascade ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design

Abstract In order to study the effects of water deficit stress and foliar application of salicylic acid (SA) on the activity of five antioxidant enzymes (catalase - CAT; EC 1.11.1.6, ascorbate peroxidase - APX; EC 1.11.1.11, glutathione reductase - GR; EC 1.6.4.2, peroxidase - POD; EC 1.11.1.7 and polyphenol oxidase - PPO; 1.14.18.1) of Thymus daenensis (subsp. lancifolius), an experiment was conducted in factorial based on completely randomized design with three replicates, during 2013. Drought treated seedlings showed elevated levels of reactive oxygen species (ROSs), with a concomitant increase in the activities of the enzymes CAT, APX, GR, POD and PPO, compared to controls. Under medium water deficit, APX and PPO activities significantly increased by higher SA concentration (2 mM), but under control and sever water deficit conditions, there was no significant difference between 1 mM and 2 mM concentrations regarding APX and PPO activity. Under all levels of available water, increase in SA concentration from 0.1 mM to1 mM induced significant increase in GR activity. The maximum amount of GR (under medium water deficit condition) achieved from 1mM of SA. While the maximum amounts of APX, PPO (under medium water deficit condition), CAT and POD (under sever water deficit condition) achieved from 2 mM of SA. In total, our results suggest that application of SA (as a trigger of signal cascade) could be advantageous against water deficit stress, and could protect thyme plants in mentioned conditions.

scholarly journals Intensity and duration of water deficit on the pathosystem sugarcane x Meloidogyne incognita

2015 ◽  
Vol 19 (6) ◽  
pp. 581-586 ◽  
Author(s):  
Matheus P. Quintela ◽  
Elvira M. R. Pedrosa ◽  
Lilia Willadino ◽  
Mario M. Rolim ◽  
Ênio F. de F. e Silva ◽  
...  
Keyword(s):  
Water Stress ◽  
Drought Stress ◽  
Water Deficit ◽  
Ascorbate Peroxidase ◽  
Peroxidase Activity ◽  
Meloidogyne Incognita ◽  
Sugarcane Variety ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Water Treatments

ABSTRACT This study aimed to evaluate the interaction between intensity and duration of water deficit (90, 56 and 22% of pot capacity [PC] for 30, 60 and 90 days under continuous stress) associated to the parasitism of the nematode Meloidogyne incognita on the growth of the sugarcane variety RB92579 and the activity of the enzymes catalase and ascorbate peroxidase. The experiment was conducted in completely randomized design in a 7 x 2 factorial scheme (seven water deficit treatments: control [90% PC], 56% PC for 30, 60 and 90 days, 20% PC for 30, 60 and 90 days; and two densities of M. incognita: 0 and 20000 eggs plant-1), with four replicates. The water stress corresponding to 56% PC for 30 or 60 days did not affect RB92579 development. The evaluated water treatments increased ascorbate peroxidase activity, but it did not affect catalase activity. Nematode inoculation did not affect RB92579 responses to drought stress conditions. The higher severity of water deficit (22% PC for 90 days) reduced M. incognita reproduction.

scholarly journals Improving water deficit tolerance of Salvia officinalis L. using putrescine

2021 ◽  
Author(s):  
Maryam Mohammadi-Cheraghabadi ◽  
Seyed Ali Mohammad Modarres-Sanavy ◽  
Fatemeh Sefidkon ◽  
Sajad Rashidi-Monfared ◽  
Ali Mokhtassi-Bidgoli
Keyword(s):  
Water Deficit ◽  
Foliar Application ◽  
Pcr Analysis ◽  
Water Deficit Stress ◽  
Irrigation Regime ◽  
Randomized Design ◽  
Increasing Trend ◽  
Completely Randomized Design ◽  
Salvia Officinalis L ◽  
Sabinene Synthase

Abstract To study the effects of foliar application of putrescine (distilled water (0), 0.75, 1.5, and 2.25 mM) and water deficit stress (20%, 40%, 60%, and 80% available soil water depletion (ASWD)) on the physiological, biochemical, and molecular attributes of sage, a factorial experiment was performed in a completely randomized design with three replications in the growth chamber. The results of qRT-PCR analysis showed that putrescine concentration, irrigation regime, and the two-way interaction between irrigation regime and putrescine concentration significantly influenced cineole synthase, sabinene synthase, and bornyl diphosphate synthase relative expression. The highest concentration of 1,8-cineole, camphor, α-thujone, β-thujone, cineole synthase, sabinene synthase, and bornyl diphosphate synthase were obtained in the irrigation regime of 80% ASWD with the application of 0.75 mM putrescine. There was high correlation between expression levels of the main monoterpenes synthase and the concentration of main monoterpenes. The observed correlation between the two enzyme activities of APX and CAT strongly suggests they have coordinated action. On the other hand, the highest PO and SOD concentrations were obtained with the application of 0.75 mM putrescine under the irrigation regime of 40% ASWD. Putrescine showed a significant increase in LAI and RWC under water deficit stress. There was an increasing trend in endogenous putrescine when putrescine concentration was increased in all irrigation regimes. Overall, the results suggest that putrescine may act directly as a stress-protecting compound and reduced H2O2 to moderate the capacity of the antioxidative system, maintain the membrane stability, and increase secondary metabolites under water deficit stress.

Herba Reudeuep With Modification of Heating In Virgin Cocanut Oil

2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Wahyuzan Wahyuzan ◽  
Lukmanul Hakim ◽  
Rahmat Afrizal ◽  
Asmeri Lamona ◽  
Khairuni Khairuni ◽  
...  
Keyword(s):  
Modern Medicine ◽  
Heating Time ◽  
Agricultural Product ◽  
Average Yield ◽  
Herbal Products ◽  
The Public ◽  
Randomized Design ◽  
Herbal Plants ◽  
Medicinal Properties ◽  
Completely Randomized Design

Traditional medicine in Indonesia has been known by the public before formal health services using modern medicine. Indonesia has many type of herbal plants which have medicinal properties including dadap (Erythrina orientalis). This study aims to utilized dadap herbal liniment by modifying heating in Vigin Cocanut Oil. The study conducted in the laboratory of Agricultural Product Processing using a Factorial Completely Randomized Design (CRD). The first factor is the addition of 250 ml (M1), 375 ml (m2), and 400 ml (M3) Virgin Cocanut Oil treatment. The second factor is the heating tim of 5 minutes (L1), 10 minutes (L2), and 15 minutes (L3). Observation variable consisted of yield, Specific gravity, and organoleptic test of aroma and colored. The result showed an average yield of 87.70-91.88%. The highest yield was found in the treatment of adding 400 ml Virgin Cocanut Oil and 15 minutes of heating time. The highest type of weight was found in the treatment of addition of 325 ml Virgin Cocanut Oil and 5 minutes heating time. Based on the organoleptic scent test, the everage penalist gives a like answer. Organoleptic colors of reudeuep herbal products is according to SNI number 06-23-2006.

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