scholarly journals Particulate bioglass in the regeneration of alveolar bone in dogs: clinical, surgical and radiographic evaluations

Revista CERES ◽  
2011 ◽  
Vol 58 (2) ◽  
pp. 149-154
Author(s):  
Alexandre Couto Tsiomis ◽  
Andréa Pacheco Batista Borges ◽  
Ana Paula Falci Daibert ◽  
Tatiana Schmitz Duarte ◽  
Emily Correna Carlo Reis ◽  
...  
Keyword(s):  
Wound Healing ◽  
Bone Substitute ◽  
Alveolar Bone ◽  
Gradual Increase ◽  
Treated Group ◽  
Radiographic Examination ◽  
Control Group ◽  
X Rays ◽  
Clinical Evaluations ◽  
Radiological Analysis

Bone loss, either by trauma or other diseases, generates an increasing need for substitutes of this tissue. This study evaluated Bioglass as a bone substitute in the regeneration of the alveolar bone in mandibles of dogs by clinical, surgical and radiological analysis. Twenty-eight adult dogs were randomly separated into two equal groups. In each animal, a bone defect was created on the vestibular surface of the alveolar bone between the roots of the fourth right premolar tooth. In the treated group, the defect was immediately filled with bioglass, while in the control, it remained unfilled. Clinical evaluations were performed daily for a week, as well as x-rays immediately after surgery and at 8, 14, 21, 42, 60, 90 and 120 days post-operative. Most animals in both groups showed no signs of inflammation and wound healing was similar. Radiographic examination revealed a gradual increase of radiopacity in the region of the defect in the control group. In the treated group, initial radiopacity was higher than that of adjacent bone, decreasing until 21 days after surgery. Then it gradually increased until 120 days after surgery, when the defect became undetectable. The results showed that Bioglass integrates into bone tissue, is biocompatible and reduced the period for complete bone regeneration.

scholarly journals Effect of smoking on alveolar bone resorption

2008 ◽  
Vol 55 (2) ◽  
pp. 107-114
Author(s):  
Jelena Racunica ◽  
Vesna Ivetic ◽  
Nada Naumovic ◽  
Milanko Djuric
Keyword(s):  
Bone Resorption ◽  
Periodontal Disease ◽  
Alveolar Bone ◽  
Mean Value ◽  
Radiographic Examination ◽  
Control Group ◽  
X Rays ◽  
Periodontal Tissues ◽  
Bone Level ◽  
The Mean

Introduction: Periodontal disease is one of the most common diseases in adults. Although the cause of periodontal disease is bacterial infection from the dental plaque, the level of destruction of periodontal tissues depends on risk factors, and smoking is one of the most important ones. Aim: The aim of the present study was to determine the level of alveolar bone resorption in smokers. Materials and Methods: Radiographic examination of all present teeth was conducted in 30 smokers (12 men and 18 women) and 30 non-smokers (13 men and 17 women, control group), 20-60 years of age. Data on smoking habits, smoking period and the number of cigarettes a day were obtained using a questionnaire. The level of alveolar bone resorption was determined on retroalveolar X-rays, by measuring the distance from the amelo-cemental junction to the bone level on mesial and distal sides of each present tooth. Results: In smokers, significantly higher (p=0.00002) values of alveolar bone resorption (3.16 ? 2.07 mm) were found compared to the control group (1.72 ? 1.02 mm). In people who had been smoking for more than 15 years, significantly greater bone resorption was observed compared to those smoking for 15 years or less (p=0.00028). The interceptive relationship showed that smokers were at 2.98x greater risk (95% CI 1.04- 8.52) for the mean value of alveolar bone resorption of > 2 mm compared to non-smokers. Conclusion: The present results have shown that smoking increases alveolar bone resorption and that the period of smoking affects the level of resorption.

Ethanol Extract of Achillea fragrantissima Enhances Angiogenesis through Stimulation of VEGF Production

2020 ◽  
Vol 21 ◽  
Author(s):  
Hana M. Hammad ◽  
Amer Imraish ◽  
Maysa Al-Hussaini ◽  
Malek Zihlif ◽  
Amani A. Harb ◽  
...  
Keyword(s):  
Wound Healing ◽  
Rural Communities ◽  
Ex Vivo ◽  
Ethanol Extract ◽  
Aortic Ring ◽  
Treated Group ◽  
Control Group ◽  
Dependent Manner ◽  
Achillea Fragrantissima

Objective: Achillea fragrantissima L. (Asteraceae) is a traditionally used medicinal herb in the rural communities of Jordan. Methods: The present study evaluated the efficacy of the ethanol extract of this species on angiogenesis in both, ex vivo using rat aortic ring assay and in vivo using rat excision wound model. Results: In concentrations of 50 and 100 µg/ml, the ethanol extract showed angiogenic stimulatory effect and significantly increased length of capillary protrusions around aorta rings of about 60% in comparison to those of untreated aorta rings. In MCF-7 cells, the ethanol extract of A. fragrantissima stimulates the production of VEGF in a dose-dependent manner. 1% and 5% of ethanol extract of A. fragrantissima containing vaseline based ointment was applied on rat excision wounds for six days and was found to be effective in wound healing and maturation of the scar. Both preparations resulted in better wound healing when compared to the untreated control group and vaseline-treated group. This effect was comparable to that induced by MEBO, the positive control. Conclusion: The results indicate that A. fragrantissima has a pro-angiogenic effect, which may act through the VEGF signaling pathway.

Cold Plasma Treatment Promotes Full-thickness Healing of Skin Wounds in Murine Models

2021 ◽  
pp. 153473462110021
Author(s):  
Joon M. Jung ◽  
Hae K. Yoon ◽  
Chang J. Jung ◽  
Soo Y. Jo ◽  
Sang G. Hwang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Plasma Treatment ◽  
Cold Plasma ◽  
Smooth Muscle Actin ◽  
Treated Group ◽  
Control Group ◽  
Alpha Smooth Muscle Actin ◽  
Skin Wound Healing

Cold plasma can be beneficial for promoting skin wound healing and has a high potential of being effectively used in treating various wounds. Our aim was to verify the effect of cold plasma in accelerating wound healing and investigate its underlying mechanism in vitro and in vivo. For the in vivo experiments, 2 full-thickness dermal wounds were created in each mouse (n = 30). While one wound was exposed to 2 daily plasma treatments for 3 min, the other wound served as a control. The wounds were evaluated by imaging and histological analyses at 4, 7, and 11 days post the wound infliction process. Immunohistochemical studies were also performed at the same time points. In vitro proliferation and scratch assay using HaCaT keratinocytes and fibroblasts were performed. The expression levels of wound healing–related genes were analyzed by real-time polymerase chain reaction and western blot analysis. On day 7, the wound healing rates were 53.94% and 63.58% for the control group and the plasma-treated group, respectively. On day 11, these rates were 76.05% and 93.44% for the control and plasma-treated groups, respectively, and the difference between them was significant ( P = .039). Histological analysis demonstrated that plasma treatment promotes the formation of epidermal keratin and granular layers. Immunohistochemical studies also revealed that collagen 1, collagen 3, and alpha-smooth muscle actin appeared more abundantly in the plasma-treated group than in the control group. In vitro, the proliferation of keratinocytes was promoted by plasma exposure. Scratch assay showed that fibroblast exposure to plasma increased their migration. The expression levels of collagen 1, collagen 3, and alpha-smooth muscle actin were elevated upon plasma treatment. In conclusion, cold plasma can accelerate skin wound healing and is well tolerated.

Effects of 1% and 3% Mobe Leaf Extract Gel on Socket Wound Healing after Tooth Extraction

2021 ◽  
Vol 24 (1) ◽  
pp. 1-5
Author(s):  
Olivia Avriyanti Hanafiah ◽  
Denny Satria ◽  
Avi Syafitri
Keyword(s):  
Wound Healing ◽  
Tooth Extraction ◽  
Leaf Extract ◽  
Alveolar Bone ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
P Value ◽  
Gel Treatment

Tooth extraction is a process of removing teeth from the alveolar bone. In wound healing, fibroblast are very important cells. The main purpose of this study was to determine the effect of mobe leaf 1% and 3% extract gel (Artocarpus lakoocha) on fibroblast proliferation in post extraction tooth socket wound healing. This research used 16 samples of wistar rats, divided into 4 groups, a positive control group, a negative control group and a 1% and 3% mobe leaf extract gel group. The left mandibular incisors were extracted, then 1% and 3% gels of mobe leaf extract were applied on day 1 to day 7. Data analysis was calculated using the Kruskal-Wallis test on clinical data and one way ANOVA test for microscopic. The result of the socket wound healing activity test for a good concentration of mobe leaf extract gel was 3%. This research shows significant resultith p-value of 0.018 (< 0.05) on the closure of the socket wound clinically which means the closure of the wound accelerates because of the mobe leaf 3% extract gel treatment. The distance of fibroblast on microscopically shows significant resultith a p-value of 0.002 (< 0.05), which means that there was an enlargement of the distance fibroblast at the socket wound closure with application of mobe leaf 3% extract gel. From the results of the study it can be concluded that mobe leaf 3% extract gel has the best ability to show acceleration the closure of the socket wound either clinically or microscopically.

scholarly journals Radiodensitometric Analysis of Maxillary Sinus-Lift Areas Enforced with Bone Substitute Materials Containing Calcium Phophate

2012 ◽  
Vol 12 (1) ◽  
pp. 41-44
Author(s):  
Laura Neimane ◽  
Andrejs Skagers ◽  
Girts Salms ◽  
Liga Berzina-Cimdina
Keyword(s):  
Maxillary Sinus ◽  
Bone Substitute ◽  
Alveolar Bone ◽  
Control Group ◽  
Study Group ◽  
Sinus Lift ◽  
Natural Bone ◽  
One Stage ◽  
Bone Substitute Materials ◽  
Substitute Materials

SummaryIntroduction. An enforcement of atrophic maxilla with biomaterials during sinus-lift surgery improves size and quality of alveolar bone. Radiological densitometric analysis can prove it. Within the study a density of bone was investigated and findings were compared with natural bone around the implants.Aim of the Study.Was to determine if density around the implant inserted with one stage sinus-lift operation using bone substitute materials increase and if that can be detected radiologically.Materials and Methods.Totally there were examined 22 patients where 64 implants were inserted in maxilla: 48 implants were inserted with one stage sinus-lift operation using bone substitute materials; represented study group. Sixteen implants were inserted in natural maxillary alveolar bone of the same patients, representing control group. The density of implant supporting tissue was measured with cone beam computed tomography and expressed in Hounsfield units (HU). The measurements were performed according to standardized pattern.Results.Densitometric measurements were higher in the study group than in the control group in all points, but statistically significant difference was observed in two measurement sites: buccally in sinus elevation area (BSM) (p=0.005) and palatinally in sinus elevation area (PSM) (p=0.0012), and corresponding areas in control group.Conclusions.Surgical elevation of maxillary sinus floor using calcium phosphate bone substitute materials resulted in higher optical density compared to natural bone. Mineralization of the bone, induced by the bone substitute materials, can be detected radiologically

scholarly journals Therapeutic Effects of Acupuncture through Enhancement of Functional Angiogenesis and Granulogenesis in Rat Wound Healing

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Sang In Park ◽  
Yun-Young Sunwoo ◽  
Yu Jin Jung ◽  
Woo Chul Chang ◽  
Moon-Seo Park ◽  
...  
Keyword(s):  
Wound Healing ◽  
Collagen Type ◽  
Interleukin 1 ◽  
Treated Group ◽  
Collagen Type I ◽  
Control Group ◽  
Therapeutic Effects ◽  
Type I ◽  
Matrix Remodeling ◽  
Cd 31

Acupuncture regulates inflammation process and growth factors by increasing blood circulation in affected areas. In this study, we examined whether acupuncture has an effect on wound healing in injured rat. Rats were assigned randomly into two groups: control group and acupuncture group. Acupuncture treatment was carried out at 8 sites around the wounded area. We analyzed the wound area, inflammatory cytokines, proliferation of resident cells, and angiogenesis and induction of extracelluar matrix remodeling. At 7 days after-wounding the wound size in acupuncture-treat group was decreased more significantly compared to control group. In addition, the protein levels of proinflammatory cytokines such as tumor necrosis factor-α(TNF-α) and interleukin-1β(IL-1β) were significantly decreased compared to the control at 2 and 7 days post-wounding. Also, we analyzed newly generated cells by performing immunostaining for PCNA and using several phenotype markers such as CD-31,α-SMA, and collagen type I. In acupuncture-treated group, PCNA-positive cell was increased and PCNA labeled CD-31-positive vessels,α-SMA- and collagen type I-positive fibroblastic cells, were increased compared to the control group at 7 days post-wounding. These results suggest that acupuncture may improve wound healing through decreasing pro-inflammatory response, increasing cell proliferation and angiogenesis, and inducing extracellular matrix remodeling.

scholarly journals Wound-healing effect of Platycodon grandiflorus (Jacq.) extract in rats

2021 ◽  
Vol 18 (9) ◽  
pp. 1909-1912
Author(s):  
Liang Wang ◽  
Li Yang ◽  
Gang Yuan ◽  
Long-Jiao Hu
Keyword(s):  
Wound Healing ◽  
Treated Group ◽  
Control Group ◽  
Type Iii Collagen ◽  
Matrix Metalloprotease ◽  
Wound Area ◽  
Burn Wounds ◽  
Type Iii ◽  
Healing Effect ◽  
Wound Tissue

Purpose: To evaluate the healing effect of Platycodon grandiflorus (Jacq.). extract (PGE) on experimental burn wounds in rats. Methods: Rats were randomly divided into four groups of eight rats each: control group, silver sulfadiazine (SSD)-treated group, moist exposed burn ointment (MEBO)-treated group and PGE-treated group. PGE, SSD and MEBO were applied topically twice daily for 7 days. SSD and MEBO were used as reference control. External observation of wound area contraction and histological analysis of wound tissues was performed respectively. The effect of PGE on matrix metalloproteases (MMPs), vascular endothelial growth factor (VEGF) and Type-III Collagen proteins of wound tissue in rats were analysed by Western blot. Results: After 10 days of topical treatment with PGE, PGE-treated group showed faster reduction in wound area when compared with control groups (p < 0.01). Matrix metalloprotease-2 (MMP-2), matrix metalloprotease-9 (MMP-9), VEGF and type-III collagen expressions in the wound tissue increased significantly (p < 0.05) when compared with the control burn wounds. Histological results showed an overall early recovery and regeneration in PGE-treated group when compared with control group. Conclusion: PGE possesses a significant wound-healing activity in full-thickness burn wounds in rats. Therefore, it can potentially be developed for the management of burns.

The Effects of Halofuginone on Wound Healing in the Rat Nasal Mucosa

2019 ◽  
Vol 34 (1) ◽  
pp. 9-15 ◽  
Author(s):  
Seyit Mehmet Ceylan ◽  
Erdal Uysal ◽  
Mehmet Sokucu ◽  
Efe Sezgin ◽  
Mahmut Alper Kanmaz ◽  
...  
Keyword(s):  
Wound Healing ◽  
Nasal Mucosa ◽  
Goblet Cell ◽  
Cell Formation ◽  
Treated Group ◽  
Potential Effect ◽  
The Other ◽  
Parasitic Infections ◽  
Control Group ◽  
Cell Hyperplasia

Background Halofuginone is an alkaloid febrifugine analogue and bioactive molecule that was isolated incidentally from the Dichroa febrifuga plant. The therapeutic efficacy of halofuginone in parasitic infections, scleroderma, inflammation, and fibrosis-related diseases, as well as in some types of cancer, has been previously reported. The effects of halofuginone on nasal mucosal damage are not yet known. Objective The aim of this study was to investigate the potential effect of topically applied halofuginone on wound healing in the mechanically injured nasal mucosa of rats. Methods A unilateral mucosal wound was created in the nasal cavity of 32 rats (aged 4 weeks) using the brushing technique. These rats were randomly divided into 4 groups. Although the control group did not receive an intervention, a dry pad, a saline-impregnated pad, or a pad impregnated with halofuginone were placed in the rats of the other 3 groups and left for 5 minutes. Rats were sacrificed on the 14th day, and a histological examination was performed. The nasal mucosa was assessed via hematoxylin-eosin and Masson’s trichrome staining. Results There were no statistically significant differences in epithelial thickness, inflammation, goblet cell formation, and epithelial disarray values between the halofuginone group and the control group ( P > .05). The subepithelial thickness was significantly decreased in the saline-treated group and the halofuginone-treated group ( P < .05), but a significantly lower level of subepithelial fibrosis was only observed in the halofuginone group compared to the other groups ( P < .05). Conclusions Topical halofuginone administration reduces the development of fibrosis and subepithelial edema after experimentally induced nasal mucosal injury, but it does not exert therapeutic or preventive effects on epithelial damage, inflammation, and goblet cell hyperplasia.

scholarly journals Wound Healing Effect of Nonthermal Atmospheric Pressure Plasma Jet on a Rat Burn Wound Model: A Preliminary Study

2019 ◽  
Vol 40 (6) ◽  
pp. 923-929 ◽  
Author(s):  
Yoonje Lee ◽  
Sanjaya Ricky ◽  
Tae Ho Lim ◽  
Ki-Seok Jang ◽  
Hongjung Kim ◽  
...  
Keyword(s):  
Wound Healing ◽  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Plasma Jet ◽  
Treated Group ◽  
Control Group ◽  
Atmospheric Pressure Plasma Jet ◽  
Plasma Medicine ◽  
Burn Wound ◽  
Burn Wounds

Abstract Worldwide, an estimated 6 million patients seek medical attention for burns annually. Various treatment methods and materials have been investigated and developed to enhance burn wound healing. Recently, a new technology, plasma medicine, has emerged to offer new solutions in wound care. As the development of plasma medicine has shown benefit in wound healing, we aimed to assess the effects of plasma medicine on burn wounds. To investigate the effectiveness of a nonthermal atmospheric pressure plasma jet (NAPPJ) for burn wound treatment on a brass comb burn wound rat model. Burn wounds were made by applying a preheated brass comb (100°C) for 2 minutes, which resulted in four full-thickness burn wounds separated by three interspaces. Interspaces were exposed to NAPPJ treatment for 2 minutes and morphological changes and neutrophil infiltration were monitored at 0, 4, and 7 days post-wounding. The percentage of necrotic interspace was higher in the control group than in the plasma-treated group (51.8 ± 20.5% vs 31.5 ± 19.0%, P &lt; .001). Moreover, the exposure of interspace to NAPPJ greatly reduced the number of infiltrating neutrophils. In addition, the percentage of interspace that underwent full-thickness necrosis in the plasma-treated group was smaller than that in the control group (28% vs 67%). NAPPJ exposure on interspaces has a positive effect on burn wounds leading to wound healing by reducing burn injury progression.

scholarly journals Treatment of periodontal disease with guided tissue regeneration technique using a hydroxyapatite and polycaprolactone membrane

2016 ◽  
Vol 68 (6) ◽  
pp. 1413-1421 ◽  
Author(s):  
L.M.A. Martins ◽  
F.L. Valente ◽  
E.C.C. Reis ◽  
R.V. Sepúlveda ◽  
A.P.L. Perdigão ◽  
...  
Keyword(s):  
Periodontal Disease ◽  
Tissue Regeneration ◽  
Alveolar Bone ◽  
Experimental Period ◽  
Treated Group ◽  
Purulent Discharge ◽  
Control Group ◽  
Clinical Attachment Level ◽  
Periodontal Tissues ◽  
Attachment Level

ABSTRACT The aim of this study was to evaluate the use of a malleable membrane composed of hydroxyapatite (60%) and polycaprolactone (40%) as treatment of periodontal disease experimentally induced in dogs. A bone defect of standardized dimensions was created between the roots of the third and fourth premolar of 12 dogs for periodontal disease induction. Six dogs had the defect covered by the membrane and six dogs received only standard treatment for periodontal disease, also applied to dogs in the treated group. The animals were clinically monitored during the experiment. Radiographs were taken after surgery and at 60 days after treatment initiation. Clinical attachment level was also assessed in those moments. On the 60th day, dental sample of all animals, containing tooth, defect and periodontal tissues, were harvested, fixed in formalin and analyzed by microtomography and histology. During the experimental period, the animals showed no pain and purulent discharge, however, there was dehiscence in 50% of animals and membrane exposure in five out of six animals in the treated group. Clinical attachment level showed no difference between groups. Radiographs showed radiopacity equal to the alveolar bone in both groups. The microtomography revealed that the control group had higher bone volume in the defect compared to the treated group; however, the furcation was not filled by new alveolar bone in any animal. Histological analysis revealed that junctional epithelium invasion was lighter in the control group. New bone was only observed in the apical edge of the defect in both groups. Although the composite is biocompatible and able to keep the space of the defect, it did not promote periodontal tissue regeneration within 60 days of observation.

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