scholarly journals New Polish cultivars of flax resistant to Fusarium wilt

2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 565-568
Author(s):  
A. Andruszewska ◽  
K. Langner ◽  
M. Byczyńska
Keyword(s):  
Fusarium Wilt ◽  
Resistance Genes ◽  
Climatic Conditions ◽  
High Yield ◽  
High Quality ◽  
Breeding Lines ◽  
Main Factors ◽  
High Yields ◽  
New Cultivars

The breeders consider the lack of resistance of fibrous flax cultivars to Fusarium wilt the main problem in flax cultivation in Poland. The resistance to diseases, lodging, the quality of fiber and adaptation to local climatic conditions are the main factors that have to be taken into consideration when high yields are involved. Therefore, the selected cultivars must be characterized by these features. In breeding process, conducted at the Institute of Natural Fibres in Poznań, the breeding lines of flax were tested in F4, F5, F8 and F10 in different years. The tests were conducted in provocative conditions. The main aim of breeding of flax is obtaining of new cultivars having the resistance genes to Fusarium wilt and genes responsible for high yields. Such genotypes were obtained from crossings between cultivars grown in Poland and cultivars characterized by high quality of fiber, high yield of seeds, resistance to Fusarium wilt and lodging. Five among many cultivars obtained at the INF show the best features. These cultivars are: Nike, Artemida, Modran, Selena and Luna.

Interaction of Physiology Character - Secondary Metabolic of Fusarium oxysporum on Tomatoes of Fusarium Wilt Symptom

2019 ◽  
Vol 967 ◽  
pp. 95-100
Author(s):  
Abdul Azis Ambar ◽  
Henny Setyawati ◽  
Nur Ilmi
Keyword(s):  
High Performance Liquid Chromatography ◽  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
High Performance ◽  
Secondary Compounds ◽  
High Quality ◽  
Code Analysis ◽  
Wilt Symptom ◽  
The Relationship

Fusarium oxysporum (F. oxysporum) is the pathogen that caused fusarium wilt diseases on the tomatoes. The rise of the symptom was caused by secondary metabolic produced by F. oxysporum. The associated with the level of secondary metabolic pathogenesis that cause symptoms of wilt on tomatoes, but secondary metabolic excreted by F. oxysporum was not necessarily wilt in a plant. The phenomenon caused F. oxysporum producing secondary metabolic in the different concentration, either its quantity or quality. The nature of physiology being tested, observed by growing 4 isolates using a medium PDA on the temperature of 10, 15, 20, 25, 30, and 35 °C. The content of secondary metabolic measured on the four isolates using the Notz et al., (2002) and analyzed by using High-Performance Liquid Chromatography (HPLC). The results of the analysis of the nature of the physiological (colour colonies) were that every isolate planted for 8 days shows the variation of white, white redness, white yellowness until purpleness. Based on Methuen Handbook of Colour (Kornerup & Wanscher, 1978), generally isolates tested shows a bright colour, signaled with A letter at every code. Analysis HPLC on the results of secondary metabolic, either quantity or quality, sequentially were: isolates BAR (3; 1,997 ppm); ENR (3; 5,105 ppm); SID (4; 2,135 ppm) and MAL (5; 2,065 rpm). If it was seen by the relationship between the colour of colonies with the production of the secondary metabolic compound, it seemed that the older or darker colonies’ colour the more secondary compounds formed, but dark or old colonies’ colour does not determine a high quality of acid fusaric produced. Keywords: Colonies colour, F. oxysporum, secondary metabolic .

scholarly journals Transcriptome and genome sequencing elucidates the molecular basis for the high yield and good quality of the hybrid rice variety Chuanyou6203

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Juansheng Ren ◽  
Fan Zhang ◽  
Fangyuan Gao ◽  
Lihua Zeng ◽  
Xianjun Lu ◽  
...  
Keyword(s):  
Molecular Mechanism ◽  
Hybrid Rice ◽  
Rice Variety ◽  
Utilization Efficiency ◽  
High Yield ◽  
Mrna Editing ◽  
High Quality ◽  
Flag Leaves ◽  
Yield And Quality

AbstractThe yield heterosis of rice is sought by farmers and strong contributes to food safety, but the quality of hybrid rice may be reduced. Therefore, developing new varieties with both high yield and good quality is a heavily researched topic in hybrid rice breeding. However, the molecular mechanism governing yield heterosis and high rice quality has not been elucidated to date. In this study, a comparative transcriptomics and genomic analysis was performed on a hybrid rice variety, Chuanyou6203 (CY6203), and its parents to investigate the molecular mechanism and gene regulation network governing the formation of yield and quality stages. A total of 66,319 SNPs and InDels between CH3203 and C106B were detected in the 5′-UTR, exon, intronic, and 3′-UTR regions according to the reference genome annotation, which involved 7473 genes. A total of 436, 70, 551, 993, and 1216 common DEGs between CY6203 and both of its parents were identified at the same stage in panicles and flag leaves. Of the common DEGs, the numbers of upregulated DEGs between CY6203 and CH3203 were all greater than those of upregulated DEGs between CY6203 and C106B in panicles and flag leaves at the booting, flowering, and middle filling stages. Approximately 40.61% of mRNA editing ratios were between 0.4 and 0.6, and 1.68% of mRNA editing events (editing ratio ≥ 0.8) in CY6203 favored one of its parents at three stages or a particular stage, suggesting that the hypothetical heterosis mechanism of CY6203 might involve dominance or epistasis. Also 15,934 DEGs were classified into 19 distinct modules that were classified into three groups by the weighted gene coexpression network analysis. Through transcriptome analysis of panicles and flag leaves in the yield and quality stages, the DEGs in the green-yellow module primarily contributed to the increase in the source of CY6203 due to an in increase in photosynthetic efficiency and nitrogen utilization efficiency, and a small number of DEGs related to the grain number added spikelet number per panicle amplified its sink. The balanced expression of the major high-quality alleles of C106B and CH3203 in CY6203 contributed to the outstanding quality of CY6203. Our transcriptome and genome analyses offer a new data set that may help to elucidate the molecular mechanism governing the yield heterosis and high quality of a hybrid rice variety.

scholarly journals Marker assisted introgression of bacterial blight resistant gene into submergence tolerance rice variety BRRI dhan52

2017 ◽  
Vol 42 (3) ◽  
pp. 403-411 ◽  
Author(s):  
ME Kabir ◽  
KM Iftekharuddaula ◽  
MAI Khan ◽  
MAK Mian ◽  
NA Ivy
Keyword(s):  
Resistance Genes ◽  
Bacterial Blight ◽  
Flash Flood ◽  
Rice Variety ◽  
High Yield ◽  
Recurrent Parent ◽  
Breeding Lines ◽  
Resistant Gene ◽  
Rainfed Lowland ◽  
Elite Breeding Lines

BRRI dhan52 is a uniquire submergence tolerant rice variety containing prominant genetic background of BR11, a mega rainfed lowland rice (RLR) variety of Bangladesh, but is susceptible to bacterial blight (BB) caused by Xanthomonas oryzae pv. oryzae (Xoo). The variety is considerably popular in the southern part of Bangladesh due to its high yield in flash flood condition and comparatvely medium slender grain. Molecular markers linked to BB resistance genes (Xa genes) and submergence QTL (SUB1) were utilized in a marker-aided selection program to develop elite breeding lines with broad-spectrum resistance to bacterial blight. Sequence tagged site (STS) and simple sequence repeat (SSR) markers were essentially used to detect the genes for BB and submergence as well. In backcross generation, markers closely linked to Xa21 and SUB1 QTL were used to select desirable plants possessing these resistance genes (foreground selection) and microsatellite markers polymorphic between donor and recurrent parent were also used to select plants that have maximum contribution from the recurrent parent genome (background selection). In BC1F1 generation, three best plants consiquently were selected from previously selected ten double heterozygous (Xa21 and SUB1 QTL) plants. The percentage of recipient genome recovery in the best plant 1, 2 and 3 were 78.7%, 75.83% and 75.4%, respectively. Eventually this work illustrates the successful application of marker-assisted breeding for introgression of bacterial blight resistant gene into a rice variety of Bangladesh.Bangladesh J. Agril. Res. 42(3): 403-411, September 2017

Efficiently Utilizing a Low Grade and Complex Copper Ore by Flotation

2013 ◽  
Vol 734-737 ◽  
pp. 929-934
Author(s):  
Qi Nie ◽  
Xiao Si Zhou ◽  
Fen Lan Peng ◽  
Xu Shen ◽  
Zhi Zhang Li
Keyword(s):  
Yunnan Province ◽  
Copper Recovery ◽  
Test Scheme ◽  
Low Grade ◽  
High Quality ◽  
Copper Ore ◽  
Copper Concentrate ◽  
Main Factors ◽  
Complex Copper

Based on the analysis of the properties of the copper ore from Jinggu area in Yunnan province, a suitable technical route was presented for processing of eligible copper concentrate and the main factors i.e. grinding fineness, Na2S dosage and collector dosage, affecting the quality of roughing concentration was investigated. On this basis, a close-circuit flotation test scheme was preceded, which obtained a high quality copper concentrate with Cu grade of 16.08%, copper recovery of 58.52%. The recovery of copper concentrate is much lower than the Cu recovery of roughing concentrate from the condition experiments. This may be contributed to the fact that fine slime carried by middling worsens the separation of copper minerals and gangues. The Mo was enriched in concentrate, which is significantly considered to recovery in further work.

scholarly journals The biotechnological potential of tomato torch, growing in Dagestan

2020 ◽  
Vol 61 (3) ◽  
pp. 131-136
Author(s):  
Saida S. Saidova ◽  
Keyword(s):  
Tomato Fruit ◽  
Climatic Conditions ◽  
Nitrogen Fertilizers ◽  
High Yield ◽  
Comparative Characteristic ◽  
Mineral Fertilizers ◽  
Tomato Plants ◽  
Biotechnological Potential ◽  
Phosphorus And Potassium

The article explores the biotechnological potential of growing Torch tomatoes in the territory of Dagestan. The characteristics of the hybrid varieties of tomatoes Torch are given. The comparative characteristic of yield indicators of tomato varieties Torch with other varieties cultivated in the southern part of Russia is presented. Five universal soil fertilizer schemes and their application regimes for growing tomatoes were tested as part of the field experiment. The most optimal ratios of the application of nitrogen, phosphorus and potassium for fertilizing soils and fertilizing plants, as an alternative to natural organic nitrogen fertilizers. It is proved that with the introduction of mineral fertilizers against the background of the aftereffect of manure in the soil, the content of nitrates, mobile phosphorus and exchange potassium increases. The influence of the timing of the use of fertilizers on the accumulation of dry matter and the area of the assimilation surface of tomatoes of the Torch variety during the growing season was studied. The dependence of the yield of commodity fruits of the Torch tomato on the doses and timing of the use of fertilizers was established. The effect of fertilizers on individual biometric indicators of Torch tomato plants on the period of mass ripening of fruits is shown; individual indicators of the quality of the Torch tomato fruit in full ripeness are determined depending on fertilizers. It has been shown that the application of fertilizers ensures the yield of fruits of sown tomatoes of the Torch cultivar at the level of 480-500 centner, and also that the tomato plants of the Torch cultivar form the maximum assimilation surface during the period of mass fruit formation. It is shown that the amount of nitrates in the fruits, regardless of the background of cultivation, does not exceed their maximum permissible concentration; in practice, the high yield of tomatoes Torch cultivar in the climatic conditions of Dagestan has been proved.

scholarly journals Monitoring of solar spectral ultraviolet irradiance in Aosta, Italy

2020 ◽  
Vol 12 (4) ◽  
pp. 2787-2810
Author(s):  
Ilias Fountoulakis ◽  
Henri Diémoz ◽  
Anna Maria Siani ◽  
Gregor Hülsen ◽  
Julian Gröbner
Keyword(s):  
Environmental Protection Agency ◽  
Climatic Conditions ◽  
Spectral Irradiance ◽  
Spatial And Temporal Variability ◽  
Spectral Measurements ◽  
High Quality ◽  
Traceability Chain ◽  
Uv Irradiance ◽  
The Alps

Abstract. A Bentham DTMc300 spectroradiometer is deployed at the station of Aosta–Saint-Christophe, Italy, at the headquarters of the Regional Environmental Protection Agency (ARPA) and has been performing continuous high-quality spectral measurements of the solar ultraviolet (UV) irradiance since 2006. The measuring site is located in the north-western region of the Alps, on a large valley floor at the altitude of 570 m a.s.l., surrounded by mountains. It is very significant to have accurate measurements in such a sensitive environment, since the complex terrain and the strongly variable meteo-climatic conditions typical of the Alps induce large spatial and temporal variability in the surface levels of the solar UV irradiance. The spectroradiometer is also used as a reference of a regional UV network, with additional stations located at different altitudes (1640 and 3500 m a.s.l.) and environmental conditions (mountain and glacier). In the present study we discuss the procedures and the technical aspects which ensure the high quality of the measurements performed by the reference instrument and the procedures used to characterize the Bentham. The quality control and quality assurance (QA–QC) procedures are also discussed. We show that the good quality of the spectral measurements is further ensured by a strong traceability chain to the irradiance scale of the Physikalisch-Technische Bundesanstalt (PTB) and a strict calibration protocol. Recently, the spectral UV dataset of Aosta–Saint-Christophe has been re-evaluated and homogenized. The final spectra constitute one of the most accurate datasets globally. At wavelengths above 310 nm and for solar zenith angles below 75∘, the expanded (k=2) uncertainty in the final dataset decreases with time, from 7 % in 2006 to 4 % in the present. The present study not only serves as the reference document for any future use of the data, but also provides useful information for experiments and novel techniques which have been applied for the characterization of the instrument and the QA–QC of the spectral UV measurements. Furthermore, the study clearly shows that maintaining a strong traceability chain to a reference scale of spectral irradiance is critical for the good quality of the measurements. The studied spectral dataset is freely accessible at https://doi.org/10.5281/zenodo.4028907 (Fountoulakis et al., 2020b).

scholarly journals A universal method for high-quality RNA extraction from plant tissues rich in starch, proteins and fiber

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Amaranatha R. Vennapusa ◽  
Impa M. Somayanda ◽  
Colleen J. Doherty ◽  
S. V. Krishna Jagadish
Keyword(s):  
Rna Extraction ◽  
Poor Quality ◽  
High Yield ◽  
Plant Tissues ◽  
Universal Method ◽  
High Quality ◽  
Yield And Quality ◽  
Extraction Buffer ◽  
Co Precipitation

Abstract Using existing protocols, RNA extracted from seeds rich in starch often results in poor quality RNA, making it inappropriate for downstream applications. Though some methods are proposed for extracting RNA from plant tissue rich in starch and other polysaccharides, they invariably yield less and poor quality RNA. In order to obtain high yield and quality RNA from seeds and other plant tissues including roots a modified SDS-LiCl method was compared with existing methods, including TRIZOL kit (Invitrogen), Plant RNeasy mini kit (Qiagen), Furtado (2014) method, and CTAB-LiCl method. Modifications in the extraction buffer and solutions used for RNA precipitation resulted in a robust method for extracting RNA in seeds and roots, where extracting quality RNA is challenging. The modified SDS-LiCl method revealed intense RNA bands through gel electrophoresis and a nanodrop spectrophotometer detected ratios of ≥ 2 and 1.8 for A260/A230 and A260/A280, respectively. The absence of starch co-precipitation during RNA extraction resulted in enhanced yield and quality of RNA with RIN values of 7–9, quantified using a bioanalyzer. The high-quality RNA obtained was demonstrated to be suitable for downstream applications, such as cDNA synthesis, gene amplification, and RT-qPCR. The method was also effective in extracting RNA from seeds of other cereals including field-grown sorghum and corn. The modified SDS-LiCl method is a robust and highly reproducible RNA extraction method for plant tissues rich in starch and other secondary metabolites. The modified SDS-LiCl method successfully extracted high yield and quality RNA from mature, developing, and germinated seeds, leaves, and roots exposed to different abiotic stresses.

scholarly journals Characteristics of Panax ginseng Cultivars in Korea and China

Molecules ◽  
2020 ◽  
Vol 25 (11) ◽  
pp. 2635 ◽  
Author(s):  
Hao Zhang ◽  
Suleman Abid ◽  
Jong Chan Ahn ◽  
Ramya Mathiyalagan ◽  
Yu-Jin Kim ◽  
...  
Keyword(s):  
Panax Ginseng ◽  
Climatic Conditions ◽  
High Yield ◽  
Future Research ◽  
Red Ginseng ◽  
High Quality ◽  
Panax Species ◽  
The World ◽  
Ginsenoside Content ◽  
Rot Disease

Ginseng (Panax ginseng Meyer) is one of the most important medicinal herbs in Asia. Its pharmacological activity comes from ginsenosides, and its roots are produced commercially for traditional and Oriental medicine. Though 17 Panax species are available around the world, there was a need to develop cultivars adapted to different climatic conditions and resistant to various diseases while still producing high-quality, high-yield roots. Thus, 12 and 9 commercial P. ginseng cultivars have been registered in South Korea and China, respectively. Those varieties show superiority to local landraces. For example, Chunpoong is more highly resistant to rusty rot disease than the local Jakyungjong landrace and has a good root shape; it is highly cultivated to produce red ginseng. The Chinese cultivar Jilin Huangguo Renshen has higher ginsenoside content than its local landraces. This review provides information about P. ginseng cultivars and offers directions for future research, such as intra- and interspecific hybridization.

scholarly journals MODERN TRENDS IN THE DEVELOPMENT OF TREATMENT AND RECREATION TOURISM IN LVIV REGION

2018 ◽  
pp. 70-76
Author(s):  
S. Zapototskyi ◽  
I. Horyn
Keyword(s):  
Low Income ◽  
Small And Medium Enterprises ◽  
Mineral Resources ◽  
Limited Range ◽  
Climatic Conditions ◽  
Mineral Waters ◽  
Recreational Services ◽  
Main Factors ◽  
Medium Enterprises

The article reveals the main features of health-improving tourism of Lviv region. The main factors of developments in the health-improving sphere of the region are noted, particularly, favourable climatic conditions, available natural mineral waters, medical mud, forest resources, etc. Sanatorium and spa establishments of Lviv region were studied. The main factors of demand formation are highlighted, among them – high prices and their non-conformity with the quality of sanatorium and resort services, low income of the population and its incapability to pay for rest in sanatorium and resort facilities, lack of effective state policy, regarding the field and the direction of support and stimulation of sanatorium and resort facilities, inefficient management system of sanatorium and resort facilities, their outdated, insufficient level of advertising support and limited range of tourist and recreational services as well as insufficient quality of additional services. Tourist flows of Lviv region resort cities are studied. According to the data, in 2017, among all the cities of Lviv region, the largest number of tourists was observed in Truskavets, Lviv, Boryslav and Morshyn – about 3 million. Among them – citizens of Poland, Azerbaijan, Moldova, Belarus, Israel, Germany and other countries. The article analyzes institutions of accommodation of largest balneological resorts of the region and the cost of living in them. The main advantages of resort area in studied settlements of the region are highlighted, in particular, its advantageous geographical position, favourable climatic conditions, availability of mineral resources, rich historical and cultural heritage as well as presence of small and medium enterprises, development of trade and services, and a large number of sanatorium and resort establishments, mostly privately owned. These can also bolster the field, considering the wide price range for such establishments. Altogether these factors further signify an already established notion – “Lviv region is a major tourist hub”.

scholarly journals Research Results for a New Onion Seed Drill

2020 ◽  
Vol 14 (4) ◽  
pp. 12-16
Author(s):  
A. A. Ibragimov ◽  
A. К. Karakhanov ◽  
A. A. Abdurakhmanov ◽  
A. Е. Eshdavlatov ◽  
P. A. Uteniyazov ◽  
...  
Keyword(s):  
Climatic Conditions ◽  
Active Part ◽  
Vegetable Crops ◽  
Seeding Rate ◽  
High Quality ◽  
Simultaneous Formation ◽  
Local Soil ◽  
Before And After ◽  
The Republic

. For sowing small seeds of vegetables, in particular onions, special seeders are used. They are not produced in the Republic of Uzbekistan, while many diff erent pneumatic seeders are produced abroad. The authors note that foreign samples are complex in design, not adapted to local soil and climatic conditions, cannot provide even seed placement on ridges, and the cost of the seeders themselves and service are very high.(Research purpose) To develop a vegetable seeder in relation to the soil and climatic conditions of Uzbekistan, to determine the seeding rate of onion seeds depending on the length of the active part of the seed reel, to evaluate the formation quality of sowing ridges and irrigation furrows.(Materials and methods) The authors determined the sowing rate by turning the seeder drive wheel at a given length of the active part of the sowing reel and collecting the sown seeds in cups with further weighing. The quality of sowing ridges and irrigation furrows formation was evaluated by the method of profi ling the fi eld before and after the seeder’s pass.(Results and discussion) The authors developed a vegetable seeder that performed three operations in one pass: cutting irrigation furrows, forming sowing ridges of a trapezoidal shape and sowing onion seeds and other small-seeded vegetable crops in a three-row tape method in each tape. They found that the dependence of the seeding rate on the length of the active part of the reel had a slightly fragile parabolic shape, and the required seeding rates – 24-48 pieces per linear meter (3.9-7.8 kilograms per hectare) – were provided with the length of the sowing reel 3.3- 6.2 millimeters. It was proved that the seeder provided a high-quality formation of sowing ridges and irrigation furrows: the row spacing was 68.8 centimeters (installation spacing – 70 centimeters), the top ridge width was 42.5 centimeters (the predetermined one was 40 centimeters), the depth of irrigation furrows was on average 9.6 centimeters.(Conclusions) A vegetable seeder was developed for sowing small-seeded vegetable crops with the simultaneous formation of sowing ridges and cutting irrigation furrows, which ensured high-quality performance of all operations and observance of onion seeds.

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