scholarly journals Characteristics of Brown Seaweeds Sargassum policystum and Padina minor from Pohuwato Water, Gorontalo

2018 ◽  
Vol 21 (3) ◽  
pp. 296
Author(s):  
Shindy Hamidah Manteu ◽  
Nurjanah Nurjanah ◽  
Tati Nurhayati
Keyword(s):  
Heavy Metals ◽  
Antioxidant Activity ◽  
Water Content ◽  
Brown Seaweed ◽  
Ethanol Extract ◽  
Proximate Analysis ◽  
Brown Seaweeds ◽  
Hypertensive Patients ◽  
Low Levels ◽  
Metals Extraction

Brown seaweed is one of the abundant resources that grows on the reefs of Pohuwato, Gorontalo Province. This research was aimed to determine the characteristics of <em>S. polycystum</em> and <em>P. minor</em> as sources of salt for hypertensive patients. The research consisted of proximate analysis, minerals, heavy metals, extraction, phytochemicals, phenols analysis, and antioxidant activity. <em>S. polycystum, P. minor</em> had water content of 17.69-22.31%, ash 24.51-30.53%, fat 0.50-0.52%, protein 3.65-4.78%, carbohydrates 53.66-41.88 and crude fiber 3.81-6.52%. The seaweeds also contained  Mg 8.89-22.41 mg/g, Fe 0.50-1.00 mg/g, K 26.90-32.71%, Na 22.23-22.69 mg/g, Ca 18.06-32.91 mg/g and ratio Na:K 0.69-0.83 mg/g. Heavy metals were detected in low levels with the content&lt;0.002-&lt;0.004 ppm. Ethanol extract of S. polycystum contained flavonoid, saponin, streoid, alkaloid. Meanwhile ethanol extract of <em>P. minor</em> contained flavonoid, saponin, triterpenoid, stereoid, phenol, dan alkaloid. Total phenol level of<em> S. polycystum</em> was 173.6 mg GAE/g and <em>P. minor</em> was 568.7 mg GAE/g. The antioxidant IC50 of <em>S. Polycystum</em> was 77.58 mg/L while <em>P. minor</em> was 66.38 mg/L.

scholarly journals Characteristics of Green Seaweeds from Seribu Islands and Sekotong West Nusa Tenggara Antioxidant

2017 ◽  
Vol 20 (3) ◽  
pp. 620 ◽  
Author(s):  
Chairun Nufus ◽  
N. Nurjanah ◽  
Asadatun Abdullah
Keyword(s):  
Heavy Metals ◽  
Antioxidant Activity ◽  
Metal Content ◽  
Antioxidant Activities ◽  
Ethanol Extract ◽  
Proximate Analysis ◽  
Caulerpa Lentillifera ◽  
Ic50 Values ◽  
Green Seaweed ◽  
Phytochemical Compounds

Green seaweeds contain many bioactive compounds which one of them act as antioxidants as well as rich in fibers and essential minerals. This study was aimed to determine physicochemicals properties of Caulerpa lentillifera, Halimeda opuntia and Ulva lactuca and as well as their antioxidant activities. Research consists of proximate analysis, mineral composition, phytochemical compounds, heavy metal content and<br />antioxidant activity of extract green seaweed by CUPRAC, DPPH and FRAP method. The results of the study macronutrient analysis shows the moisture content ranged from 10.83% to 28.41%, ash 24.97% to 70.66%, fat 3.80% to 5.26%, fiber 2.63% to 3.86%, protein 0.18% to 1.43% and carbohydrate 10.69% to 36.49%. Mineral Ca C. lentillifera, H. opuntia and U.  lactuca about 17, 95-124,39 g/kg, Fe 0,13-0,34 g/kg,<br />K 2,29-12,28 g/kg, Mg 2.63-22.23 g/kg and Na 21.16-34.18 g/kg. The heavy metals content of C. lentillifera, H. opuntia and U.  lactuca ranged in Pb 13.57 mg/kg to 37.76 mg/kg, Cu 5.02 mg/kg to 8.32 mg/kg and Hg 0.170 mg/kg to 0.265 mg/kg. Phytochemicals properties of C. lentillifera, H. opuntia and U.  lactuca were<br />alkaloids, flavonoids, phenols hydroquinone, saponins, steroids and triterpenoids. Antioxidant activity of ethanol extract DPPH showed IC50 values ranged from 1.6–4 445 mg /L, CUPRAC 138.21–212.43 μmol trolox/g extract, FRAP 175.93–181.79 μmol trolox/g extract. <br /><br />

scholarly journals Antioxidant Activities of Various Brown Seaweeds from Seribu Islands

2017 ◽  
Vol 20 (2) ◽  
pp. 305 ◽  
Author(s):  
Seftylia Diachanty ◽  
N. Nurjanah ◽  
Asadatun Abdullah
Keyword(s):  
Heavy Metals ◽  
Antioxidant Activities ◽  
Chemical Properties ◽  
Ethanol Extract ◽  
Proximate Analysis ◽  
Crude Fiber ◽  
Total Phenolic ◽  
Brown Seaweeds ◽  
Antioxidants Activity ◽  
Ic50 Values

Brown seaweeds contain various metabolites (carotenoids, laminarin, alginate, fucoidan, mannitol, and phlorotannin) as anticancer substances, antioxidants and chemopreventive agent against degenerative diseases. This research was aimed to determine the chemical properties and antioxidants activity of Sargassum polycystum, Padina minor and Turbinaria conoides. This research consisted of the proximate analysis, crude fiber, minerals, heavy metals, extraction, phytochemicals, analysis phenolic compound and antioxidant activity with DPPH, FRAP and CUPRAC methods. The results showed moisture content of S. polycystum, P.  minor, and T. conoides 24-31%, fat 0.23-0,47%, protein 3-4%, ash 27-45%, carbohydrate 26-38% and crude fiber 2-6%. Mineral Fe S. polycystum, P.minor and T.conoides about 0.03 g/kg to 0.39 g/kg, Ca 11.28 g/kg to 27.98 g/kg, K 13.86 g/kg to 41.15 g/kg, Na 16.31 g/kg to 20.51 g/kg and Mg 8.04 g/ kg to 18.68 g/kg. The range of heavy metals Pb S. polycystum, P.  m i n o r  and T. conoides 3.48 mg/kg to 12.68 mg/kg, Hg 0.24 mg/kg to 0.53 mg/kg and Cu 5.66 mg/kg to 7.02 mg/kg. Ethanol extract of S. polycystum, P. minor, and T. conoides contains alkaloids, phenolic, flavonoids, saponins, steroids, and triterpenoids. Total phenolic compounds of brown seaweeds ranged from 3758.97-8287.18 mg GAE/g. Brown seaweeds contain DPPH antioxidant activities within IC50 values of 1.9-9.6 mg/mL, FRAP 70.643-105.357 µmol Trolox/g and CUPRAC 85.268-201 µmol Trolox/g.

scholarly journals Antioxidant Activity Analysis and Standardization of Parkia speciosa (Petai) Pods Ethanol Extract

2019 ◽  
Vol 4 (1) ◽  
pp. 5 ◽  
Author(s):  
Najma Annuria Fithri ◽  
Fitrya Fitrya ◽  
Tia Shabrina ◽  
Diva Yulanri
Keyword(s):  
Antioxidant Activity ◽  
Water Content ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Structural Stabilization ◽  
Dpph Method ◽  
Research Outcome ◽  
Phenolic And Flavonoid

Parkia speciosa (petai) pods are rarely used and considered as waste despite their phenolic and flavonoid content. Phenolic and flavonoid content in plants are known to exhibit antioxidant activity. Antioxidants are known to potentially act agains radical and mediate the damage by structural stabilization. This research was conducted to analyze the antioxidant potential of petai pods using DPPH method. Analysis showed the IC50 of petai pods ethanolic extract was 75.72 ppm, which indicate strong acting antioxidant. Standardization of extracts were determined to manage the quality of extract, including the analysis of density, water content, ash values, and quantification of total phenolic and flavonoid content. Research outcome indicate the extract retain its quality during storage and use which confrmed to standardization requirements of water content and ash values. Total phenolics and flavonoid obtained were 272.45 mg/gGAE and 243.2029 mg/gQE respectively. Based from this research, petai pods ethanolic extract present strong antioxidant activity which was contributed by high existing content of flavonoid and phenolic compounds.

scholarly journals Antioxidant Activity and Proximate Analysis of Dry Powder from Brown Seaweed Sargassum hystrix

2017 ◽  
Vol 19 (1) ◽  
pp. 29
Author(s):  
Radipta Lailatussifa ◽  
Amir Husni ◽  
Alim Isnansetyo
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Brown Seaweed ◽  
Water Extract ◽  
Proximate Analysis ◽  
Total Phenolic ◽  
Dry Powder ◽  
Nutrient Contents

This research aims to know antioxidant activity, total phenolic, nutrient, and screening phytochemistry compounds of S. hystrix dry powder. Water extract from the dried sample of brown algae (S. hystrix) was prepared and examined for its phenolic compounds. The amount of total phenolic compounds in water extract was about 11.43 g Gallic Acid Equivalent (GAE)/100 g of dry basis sample, as measured by using Folin-Ciocalteu method. The antioxidant activity (Radical Scavenging Activity/RSA) of BHT as a standard was high about 96% inhibition of radical DPPH with 1 mg dry sample/ml solvent. The IC50 of the BHT was 0.227 ± 0.001 mg of dry sample/ ml solvent. While the radical activity (RSA) of S. hystrix dry powder was about 65.28% at 1 mg dry sample/ml solvent. The IC50 of S. hystrix dry powder was 0.616 ± 0.005 mg of dry sample/ml solvent. Nutrient contents and bioactive compounds of dry powder S. hystrix were balanced to support antioxidant activity of the sample. The results suggest that this alga is a potential source of natural antioxidant.

scholarly journals The Effect of Blanching Time on Antioxidant Activity and Sensory Characteristic of Brown Seaweed Sargassum sp. Tea

2019 ◽  
Vol 22 (3) ◽  
pp. 581-588
Author(s):  
Ellya Sinurat ◽  
Th.Dwi Suryaningrum
Keyword(s):  
Antioxidant Activity ◽  
Water Content ◽  
Bioactive Compounds ◽  
Brown Seaweed ◽  
Total Phenols ◽  
Phenol Content ◽  
Sensory Characteristic ◽  
Sensory Tests

Brown seaweed Sargassum sp. is known to contain bioactive compounds needed for human. Seaweedcan be brewed into antioxidant drinks. The purpose of this study was to determine antioxidant activity andsensory properties of Sargassum sp. with variations in the duration of blanching 1, 3, and 5 minutes. Thetea was analysed for their water content, total phenols, antioxidant activity and sensory tests. The highestantioxidant activity was obtained in blanching treatment for 5 minutes with phenol content of 2.22 mg GAE/g. However, the hedonic test suggested 3 minutes blanching treatment was the best treatment to obtain theseaweed tea.

scholarly journals Aktivitas Antioksidan dan Total Polisakarida Ekstrak Rumput Laut Merah, Hijau dan Coklat dari Pantai Jangkar Situbondo

2019 ◽  
Vol 16 (2) ◽  
pp. 64
Author(s):  
Didin Erma Indahyani ◽  
Depi Praharani ◽  
Izzata Barid ◽  
Ari Tri Wanodyo Handayani
Keyword(s):  
Antioxidant Activity ◽  
Brown Seaweed ◽  
Water Extract ◽  
Ethanol Extract ◽  
Extraction Methods ◽  
Red Seaweed ◽  
Water Extracts ◽  
High Antioxidant Activity ◽  
Polysaccharide Composition ◽  
Ethanol Extracts

The antioxidant activity and polysaccharide composition of seaweed is influenced by species (red, green and brown seaweed), geographical conditions, extraction methods, solvents, temperature and the length of time of extraction. The purpose of this study was to known the total polysaccharide and antioxidant activity in red, green and brown seaweed extracts from Jangkar Situbondo beach. Method. The study was conducted on red, green and brown seaweed extracted with ethanol and water to determine total polysaccharides. Antioxidant analysis uses the method 2,2-diphenyl1-picrylhydrazyl (DPPH) radical. Total polysaccharide and antioxidant activity were observed statistically using two-way ANOVA. The results of this study show that the polysaccharide content in seaweed water extract was significantly higher than ethanol extract, especially in brown seaweed. Seaweed water extracts showed higher antioxidant activity than ethanol extracts. The antioxidant activity of brown seaweed was significantly higher than that of green and red seaweed. Conclusion. Seaweeds have high antioxidant activity in water extracts. Brown seaweed water extract contains high polysaccharides than green and red seaweed. Antioxidant activity in brown seaweed is also higher than in green and red seaweed.

scholarly journals Kandungan Gizi dan Aktivitas Antioksidan Permen Jelly Buah Pedada (Sonneratia Caseolaris) dengan Penambahan Karagenan

2020 ◽  
Vol 9 (2) ◽  
pp. 154
Author(s):  
Dwi Tria Ramadani ◽  
Dini Wulandari ◽  
Aisah Aisah
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Water Content ◽  
Fruit Juice ◽  
Proximate Analysis ◽  
Ash Content ◽  
Chemical Characteristics ◽  
Sonneratia Caseolaris ◽  
Quantitative Descriptive ◽  
Activity Method

Pedada fruit (Sonneratia caseolaris) is one of the mangrove varieties that has a high nutritional content and has the potential as an antioxidant. In general, pedada is still underutilized by the community because it tastes sour and feels tight when consumed directly. One of the efforts in utilizing the pedada fruit is processing it into jelly candy. The purpose of this study was to determine the chemical characteristics of pedada jelly candy with the addition of carrageenan. This research is an experimental design using a quantitative descriptive approach with P2K2(100 ml  of pedada fruit juice and 20 gr carrageenan) and P0K2 contol samples (without pedada fruit juice and 20 gr of carrageenan). To find out the chemical characteristics were tested Carbohydrate, protein, fat, fiber, water content, ash content, vitamin C and Antioxidant activity. This research was conducted in May-August 2018. Jelly candy making was carried out at the STIKes Baiturrahim Jambi Nutrition and Food Laboratory, Chemical characteristics were tested by proximate analysis (Carbohydrate, protein, fat, fiber, water content, ash content), iodimetry (vitamin c) method and DPPH (Antioxidant activity) method. The best treatment in making Pedada jelly candy which is very preferred, namely P2K2 (100 ml pedada fruit juice and 20 gr carrageenan) with a carbohydrates (69.83%), protein (5.66%), fat (9.89%), water content (39.26%), ash content (9.67%), fiber (0.67%), vitamin C (14.08%) and antioxidant activity (67.34%).

Antioxidant Activity, Inhibition α-Glucosidase of Ethanol Extract of Strychnos nitida G. Don and Identification of Active Compounds

2019 ◽  
Vol 5 (3) ◽  
pp. 11-20
Author(s):  
Stefani Dhale Rale ◽  
Hasim Hasim ◽  
Syamsul Falah
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Inhibition Activity ◽  
Active Compounds ◽  
Activity Inhibition ◽  
Activity Test ◽  
Ic50 Value ◽  
Treatment Of Diabetes

This study aims to find the treatment of diabetes using natural materials by exploring plants in the province of East Nusa Tenggara. his research was conducted out by extracting the Strychnos nitida G.Don stem using a method of maceration by ethanol 70%. Ethanol extract was then fractionated using n-hexane and ethyl acetate. Simplicia from maceration and fractionation results were then tested for antioxidant activity, α-glucosidase inhibition activity and identification of active compounds. The results showed that ethyl acetate fraction had the lowest IC50 value of 86.83 μg / ml. Results of the α-glucosidase activity test showed that ethyl acetate fraction and n-heksan fraction at 900 ppm had the highest percentage of inhibition of 34.23% and 33.89%. Identification using LCMS/MS method showed that ethyl acetate fraction consist of Benzenemethamine, N, N-dioctyl- as an antioxidantcompound and compound 24-methyl-5-cholestone-hexol as an antidiabetic compound. From the results of this study, we concluded that the extract of kayu ular Strychnos nitida G.Don stem has inhibition activity toward α-glucosidase enzyme.

UJI AKTIVITAS ANTIOKSIDAN FRAKSI N-HEKSAN DAUN AFRIKA (Vernonia amygdalina Del.) DENGAN METODE DPPH

2020 ◽  
Vol 5 (2) ◽  
pp. 250-257
Author(s):  
Nurul Fatimah ◽  
◽  
Reksi Sundu
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Hexane Fraction ◽  
Phytochemical Screening ◽  
Vernonia Amygdalina ◽  
Tropical Africa ◽  
Ic50 Value

Free radicals and reactive species are widely believed to contribute to the development of several diseases by causing oxidative stress and eventually oxidative. Vernonia amygdalina (Astereacea) is a small shrub or tree between 1 and 5m high growing throughout tropical Africa. Plants are generally known as bitter leaves is well cultivated and is a general market for merchandise in several countries. The purpose of this study was to determine the antioxidant activity of hexane fraction from ethanol extract od Frican leaves (Vernonia amygdalina Del.). The method used in this study was the DPPH (1,1-Diphenil-2-Picrylhydrazyl) method. The result of phytochemical screening showed that ethanolic extract of African leaves contained a composition of secondary metabolites of alkaloids, flavonoids, tannins, steroids/triterpenoids and saponins. The antioxidant activity of the extract of n-hexane fraction was classified as very weak with an IC50 value of 317.98 ppm.

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