Characteristics of Green Seaweeds from Seribu Islands and Sekotong West Nusa Tenggara Antioxidant

Green seaweeds contain many bioactive compounds which one of them act as antioxidants as well as rich in fibers and essential minerals. This study was aimed to determine physicochemicals properties of Caulerpa lentillifera, Halimeda opuntia and Ulva lactuca and as well as their antioxidant activities. Research consists of proximate analysis, mineral composition, phytochemical compounds, heavy metal content and antioxidant activity of extract green seaweed by CUPRAC, DPPH and FRAP method. The results of the study macronutrient analysis shows the moisture content ranged from 10.83% to 28.41%, ash 24.97% to 70.66%, fat 3.80% to 5.26%, fiber 2.63% to 3.86%, protein 0.18% to 1.43% and carbohydrate 10.69% to 36.49%. Mineral Ca C. lentillifera, H. opuntia and U. lactuca about 17, 95-124,39 g/kg, Fe 0,13-0,34 g/kg, K 2,29-12,28 g/kg, Mg 2.63-22.23 g/kg and Na 21.16-34.18 g/kg. The heavy metals content of C. lentillifera, H. opuntia and U. lactuca ranged in Pb 13.57 mg/kg to 37.76 mg/kg, Cu 5.02 mg/kg to 8.32 mg/kg and Hg 0.170 mg/kg to 0.265 mg/kg. Phytochemicals properties of C. lentillifera, H. opuntia and U. lactuca were alkaloids, flavonoids, phenols hydroquinone, saponins, steroids and triterpenoids. Antioxidant activity of ethanol extract DPPH showed IC50 values ranged from 1.6–4 445 mg /L, CUPRAC 138.21–212.43 μmol trolox/g extract, FRAP 175.93–181.79 μmol trolox/g extract.

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Antioxidant Activities of Various Brown Seaweeds from Seribu Islands

Jurnal Pengolahan Hasil Perikanan Indonesia ◽

10.17844/jphpi.v20i2.18013 ◽

2017 ◽

Vol 20 (2) ◽

pp. 305 ◽

Cited By ~ 9

Author(s):

Seftylia Diachanty ◽

N. Nurjanah ◽

Asadatun Abdullah

Keyword(s):

Heavy Metals ◽

Antioxidant Activities ◽

Chemical Properties ◽

Ethanol Extract ◽

Proximate Analysis ◽

Crude Fiber ◽

Total Phenolic ◽

Brown Seaweeds ◽

Antioxidants Activity ◽

Ic50 Values

Brown seaweeds contain various metabolites (carotenoids, laminarin, alginate, fucoidan, mannitol, and phlorotannin) as anticancer substances, antioxidants and chemopreventive agent against degenerative diseases. This research was aimed to determine the chemical properties and antioxidants activity of Sargassum polycystum, Padina minor and Turbinaria conoides. This research consisted of the proximate analysis, crude fiber, minerals, heavy metals, extraction, phytochemicals, analysis phenolic compound and antioxidant activity with DPPH, FRAP and CUPRAC methods. The results showed moisture content of S. polycystum, P. minor, and T. conoides 24-31%, fat 0.23-0,47%, protein 3-4%, ash 27-45%, carbohydrate 26-38% and crude fiber 2-6%. Mineral Fe S. polycystum, P.minor and T.conoides about 0.03 g/kg to 0.39 g/kg, Ca 11.28 g/kg to 27.98 g/kg, K 13.86 g/kg to 41.15 g/kg, Na 16.31 g/kg to 20.51 g/kg and Mg 8.04 g/ kg to 18.68 g/kg. The range of heavy metals Pb S. polycystum, P. m i n o r and T. conoides 3.48 mg/kg to 12.68 mg/kg, Hg 0.24 mg/kg to 0.53 mg/kg and Cu 5.66 mg/kg to 7.02 mg/kg. Ethanol extract of S. polycystum, P. minor, and T. conoides contains alkaloids, phenolic, flavonoids, saponins, steroids, and triterpenoids. Total phenolic compounds of brown seaweeds ranged from 3758.97-8287.18 mg GAE/g. Brown seaweeds contain DPPH antioxidant activities within IC50 values of 1.9-9.6 mg/mL, FRAP 70.643-105.357 µmol Trolox/g and CUPRAC 85.268-201 µmol Trolox/g.

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THE ANTIOXIDANT ACTIVITY AND PHYTOCHEMICAL SCREENING OF ETHANOL EXTRACT, FRACTIONS OF WATER, ETHYL ACETATE AND n-HEXANE FROM MISTLETOE TEA (SCURRULA ATROPURPUREABL. DANS)

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i2.15724 ◽

2017 ◽

Vol 10 (2) ◽

pp. 343 ◽

Cited By ~ 4

Author(s):

Resmi Mustarichie

Keyword(s):

Antioxidant Activity ◽

Ascorbic Acid ◽

Ethyl Acetate ◽

Antioxidant Activities ◽

Ethanol Extract ◽

Ethyl Acetate Fraction ◽

Hexane Fraction ◽

Phytochemical Screening ◽

Water Fraction ◽

Ic50 Values

Objective: The aim of this study is to investigate antioxidant activity and phytochemical screening of ethanol extract, fractions of water, ethyl acetate, and n-hexane from mistletoe tea (Scurrula atropurpurea Bl. Dans).Methods: Simplicia extracted using soxhlet equipment with 96% ethanol. Fractionation was conducted using liquid-liquid extraction using a solvent of water, ethyl acetate and n-hexane. Screening of phytochemical and antioxidant activity was performed against these fractions. Antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl method using ultraviolet-visible spectrophotometry with ascorbic acid as standard. Phytochemical screening was conducted based on the method of Farnsworth.Results: The IC50 values of ethanol extract, water fraction, fraction of ethyl acetate, and n-hexane fraction were 21.92 ppm, 89.57 ppm, 14.08 ppm, and 162.09 ppm, respectively, whereas for ascorbic acid was 4.41 ppm. The ethanol extract and ethyl acetate fraction contained compounds were the same group, polyphenolic, tannins, flavonoids, monoterpenoid, steroids, triterpenoids, and quinones. Fraction of water contained compounds such as polyphenolic group, flavonoids, monoterpenoids, sesquiterpenoids, steroids, and triterpenoids. n-hexane fraction compounds contained steroids and triterpenoids.Conclusion: The ethanol extract, water fraction, ethyl acetate fraction, and n-hexane fraction showed antioxidant activities. The ethanol extract, fractions of water, and ethyl acetate fraction contained flavonoids and polyphenolic potential as antioxidants.Keywords: Mistletoe tea, Scurrula atropurpurea, Antioxidant, 2,2-diphenyl-1-picrylhydrazyl, Ascorbic acid.

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Characteristics of Brown Seaweeds Sargassum policystum and Padina minor from Pohuwato Water, Gorontalo

Jurnal Pengolahan Hasil Perikanan Indonesia ◽

10.17844/jphpi.v21i3.24709 ◽

2018 ◽

Vol 21 (3) ◽

pp. 296

Author(s):

Shindy Hamidah Manteu ◽

Nurjanah Nurjanah ◽

Tati Nurhayati

Keyword(s):

Heavy Metals ◽

Antioxidant Activity ◽

Water Content ◽

Brown Seaweed ◽

Ethanol Extract ◽

Proximate Analysis ◽

Brown Seaweeds ◽

Hypertensive Patients ◽

Low Levels ◽

Metals Extraction

Brown seaweed is one of the abundant resources that grows on the reefs of Pohuwato, Gorontalo Province. This research was aimed to determine the characteristics of S. polycystum and P. minor as sources of salt for hypertensive patients. The research consisted of proximate analysis, minerals, heavy metals, extraction, phytochemicals, phenols analysis, and antioxidant activity. S. polycystum, P. minor had water content of 17.69-22.31%, ash 24.51-30.53%, fat 0.50-0.52%, protein 3.65-4.78%, carbohydrates 53.66-41.88 and crude fiber 3.81-6.52%. The seaweeds also contained Mg 8.89-22.41 mg/g, Fe 0.50-1.00 mg/g, K 26.90-32.71%, Na 22.23-22.69 mg/g, Ca 18.06-32.91 mg/g and ratio Na:K 0.69-0.83 mg/g. Heavy metals were detected in low levels with the content<0.002-<0.004 ppm. Ethanol extract of S. polycystum contained flavonoid, saponin, streoid, alkaloid. Meanwhile ethanol extract of P. minor contained flavonoid, saponin, triterpenoid, stereoid, phenol, dan alkaloid. Total phenol level of S. polycystum was 173.6 mg GAE/g and P. minor was 568.7 mg GAE/g. The antioxidant IC50 of S. Polycystum was 77.58 mg/L while P. minor was 66.38 mg/L.

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FORMULASI DAN UJI AKTIVITAS ANTIOKSIDAN KRIM EKSTRAK ETANOL DAUN MURBEI (Morus alba L.) MENGGUNAKAN METODE DPPH (1,1-Diphenyl-2-Picrylhydrazyl)

PHARMACON ◽

10.35799/pha.9.2020.30018 ◽

2020 ◽

Vol 9 (3) ◽

pp. 349

Author(s):

Eklesia Pogaga ◽

Paulina V. Y. Yamlean ◽

Julianri S. Lebang

Keyword(s):

Antioxidant Activity ◽

Free Radical ◽

Vitamin C ◽

Traditional Medicine ◽

Antioxidant Activities ◽

Ethanol Extract ◽

Mulberry Leaves ◽

Ic50 Value ◽

Ic50 Values ◽

Ethanol Extracts

ABSTRACTMulberry is one of the plants growing in Indonesian and widely used as traditional medicine. The active compounds found in Mulberry leaves are known have an antioxidant activity. The aim of this study is to formulate cream preparations from ethanol extracts of Mulberry leaves and to determine its antioxidant activities. Mulberry leaves was macerated using ethanol 96%. Antioxidant activity of mulberry leaves cream then evaluated against DPPH as free radical and measured at 517 nm wavelength using a UV-Vis spectrophotometer. The results showed that the cream of ethanol extract from Mulberry leaves had a very strong antioxidant activity with IC50 values in a row that is 1.7831 ppm, 0.8215 ppm and 0.7668 ppm. This can be compared with a Vitamin C solution, which has a very strong antioxidant activity with IC50 value was 1.1113 ppm. This study concludes that the cream from Mulberry leaves ethanol extract has a very strong antioxidant activity.Keywords: Mulberry leaves, Cream, Antioxidant, DPPHABSTRAK Murbei merupakan salah satu tanaman yang tumbuh di Indonesia dan banyak digunakan dalam pengobatan secara tradisional. Kandungan senyawa aktif yang terdapat pada daun Murbei dikenal mempunyai peranan sebagai antioksidan. Tujuan dari penelitian ini ialah untuk membuat sediaan krim dari ekstrak etanol daun Murbei dan untuk menguji apakah adanya aktivitas antioksidan. Daun murbei diekstraksi dengan metode maserasi menggunakan pelarut etanol 96%. Sediaan krim diuji terhadap DPPH sebagai radikal bebas dan diukur pada panjang gelombang 517 nm dengan menggunakan spektrofotometer UV-Vis. Hasil penelitian menunjukkan bahwa krim ekstrak etanol daun Murbei memiliki aktivitas antioksidan yang sangat kuat dengan nilai IC50 berturut-turut yaitu 1,7831 ppm, 0,8215 ppm dan 0,7668 ppm. Hal ini dapat dibandingkan dengan larutan Vitamin C yang mempunyai aktivitas antioksidan yang sangat kuat, yaitu 1.1113 ppm. Berdasarkan hasil penelitian dapat disimpulkan bahwa krim dari ekstrak etanol daun Murbei memiliki aktivitas antioksidan yang sangat kuat.Kata Kunci: Daun Murbei, Krim, Antioksidan, DPPH

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In-Vitro Study of Antioxidant Activities from Ethanol Extracts of Akar Kuning (Arcangelisia flava)

Jurnal Surya Medika ◽

10.33084/jsm.v4i2.594 ◽

2019 ◽

Vol 4 (2) ◽

pp. 66-71

Author(s):

Suratno Suratno ◽

Muhammad Ikhwan Rizki ◽

Mohammad Rizki Fadhil Pratama

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

In Vitro Study ◽

Ethanol Extract ◽

Antioxidant Compounds ◽

Dpph Method ◽

Ic50 Values ◽

Stem Extract ◽

Ethanol Extracts

This study aims to determine the antioxidant activity of akar kuning (Arcangelisia flava) stem extract from Central Kalimantan using 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. This research includes extraction of simplicia and test of antioxidant activity by DPPH method. Based on the analysis of antioxidant activity on ethanol extract from yellow root plant stem IC50 values of 136.81 ppm were obtained. This IC50 value indicates the antioxidant strength of the yellow root plant stems including in the moderate category. Further research on antioxidant activity in the fraction of akar kuning stems needs to be done to determine the composition of the antioxidant compounds in each solvent.

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Antidyslipidemic and Antioxidant Activities of Matricaria pubescens (Desf.) Shultz.in Streptozotocin-Induced Diabetic Rats

Cardiovascular & Hematological Agents in Medicinal Chemistry ◽

10.2174/1871525718666200506100139 ◽

2020 ◽

Vol 18 ◽

Cited By ~ 1

Author(s):

Ayoub Amssayef ◽

Bouchra Azzaou ◽

Mohammed Ajebli ◽

Mohamed Eddouks

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Serum Levels ◽

Diabetic Rats ◽

Favorable Effect ◽

Lipoprotein Profile ◽

Phytochemical Compounds ◽

Asteraceae Family

Aims: The study aimed to evaluate the antihyperlipidemic and antioxidant activities of Matricaria pubescens. Background: Matricaria pubescens (Desf.) Shultz belongs to Asteraceae family and it is commonly used traditionally for handling diabetes mellitus. Objective: The objective of this study was to assess the antioxidant activity of Matricaria pubescens (Desf.) Shultz and its effect on lipid and lipoprotein profile in normal and streptozotocin-induced diabetic rats. Methods: The effect of repeated (7 days of treatment) oral administration of the aqueous extract of aerial part of Matricaria pubescens (MPAE) at a dose of 40 mg/kg on lipid and lipoprotein profile was examined in normal and streptozotocin-induced diabetic rats. Furthermore, a preliminary phytochemical screening and the quantification of phenolic, flavonoid and tannin contents as well as the antioxidant activity using two methods (FRAP and ABTS) were carried out. Results: MPAE demonstrated a potent antidyslipidemic effect in diabetic rats by reducing serum levels of triglycerides, total cholesterol and low-density lipoprotein (LDL). In addition, the results showed that the extract is rich in several phytochemical compounds and revealed an important antioxidant activity. Conclusion: In summary, this study proved that Matricaria pubescens (Desf.) Shultz. has a favorable effect on diabetic dyslipidemia.

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Effect of particle size on phytochemical composition and antioxidant properties of Sargassum cristaefolium ethanol extract

Scientific Reports ◽

10.1038/s41598-021-95769-y ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

E. S. Prasedya ◽

A. Frediansyah ◽

N. W. R. Martyasari ◽

B. K. Ilhami ◽

A. S. Abidin ◽

...

Keyword(s):

Antioxidant Activity ◽

Particle Size ◽

Antioxidant Activities ◽

Antioxidant Properties ◽

Ethanol Extract ◽

Total Phenolic ◽

Particle Sizes ◽

Epicatechin Gallate ◽

Particle Size Reduction ◽

Phytochemical Composition

AbstractSample particle size is an important parameter in the solid–liquid extraction system of natural products for obtaining their bioactive compounds. This study evaluates the effect of sample particle size on the phytochemical composition and antioxidant activity of brown macroalgae Sargassum cristaefolium. The crude ethanol extract was extracted from dried powders of S.cristeafolium with various particle sizes (> 4000 µm, > 250 µm, > 125 µm, > 45 µm, and < 45 µm). The ethanolic extracts of S.cristaefolium were analysed for Total Phenolic Content (TPC), Total Flavonoid Content (TFC), phenolic compound concentration and antioxidant activities. The extract yield and phytochemical composition were more abundant in smaller particle sizes. Furthermore, the TPC (14.19 ± 2.08 mg GAE/g extract to 43.27 ± 2.56 mg GAE/g extract) and TFC (9.6 ± 1.8 mg QE/g extract to 70.27 ± 3.59 mg QE/g extract) values also significantly increased as particle sizes decreased. In addition, phenolic compounds epicatechin (EC), epicatechin gallate (ECG), epigallocatechin (EGC), and Epigallocatechin gallate (EGCG) concentration were frequently increased in samples of smaller particle sizes based on two-way ANOVA and Tukey’s multiple comparison analysis. These results correlate with the significantly stronger antioxidant activity in samples with smaller particle sizes. The smallest particle size (< 45 µm) demonstrated the strongest antioxidant activity based on DPPH, ABTS, hydroxyl assay and FRAP. In addition, ramp function graph evaluates the desired particle size for maximum phytochemical composition and antioxidant activity is 44 µm. In conclusion, current results show the importance of particle size reduction of macroalgae samples to increase the effectivity of its biological activity.

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IDENTIFIKASI SENYAWA FITOKIMIA DAN AKTIVITAS ANTIOKSIDAN EKSTRAK KASAR DAUN PEPE

Jurnal Ilmu dan Teknologi Pangan (ITEPA) ◽

10.24843/itepa.2019.v08.i02.p01 ◽

2019 ◽

Vol 8 (2) ◽

pp. 111

Author(s):

Dewa Gede Eka Prayoga ◽

Komang Ayu Nocianitri ◽

Ni Nyoman Puspawati

Keyword(s):

Antioxidant Activity ◽

Quantitative Data ◽

Qualitative Data ◽

Total Phenol ◽

Total Flavonoids ◽

Randomized Design ◽

Solvent Type ◽

Ic50 Values ◽

Phytochemical Compounds ◽

Completely Randomized Design

This research was conducted to identify the phytochemical compounds and antioxidant activity of pepe leaves (Gymnema reticulatum Br.) crude extract in various solvent types. The experimental design used in this research was a completely randomized design with solvent type treatments consisting of 5 types : aquadest, ethyl acetate 96%, acetone 70%, ethanol 70%, and methanol 95%. The treatment was repeated three times, so that 15 experimental units were obtained. Qualitative data obtained in this research were displayed with tables, while quantitative data were analyzed by variance and if the treatment had a significant effect, then followed by Duncan's test. The results showed that the treatment of solvent types affected phytochemical compounds that was extracted from pepe leaves and had a significant effect (P<0.01) on yield, total phenol, total flavonoids, and antioxidant activity. The best results showed that 70% acetone solvents were able to extract phytochemical compounds from alkaloids, saponins, phenols, flavonoids, and steroids in the form of sterols and had the highest antioxidant activity based on 62.06% free radical inhibition percentage with IC50 values of 200.775 mg/L, yield 32.35%, total phenol 36.14 mgGAE/g extract, and total flavonoids 50.37 mgQE/g extract.

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UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL BULBUS BAWANG DAYAK (Eleutherine americana Merr) DENGAN METODE DPPH (1,1-Diphenyl-2-Picrylhydrazyl)

PHARMACON ◽

10.35799/pha.9.2020.30031 ◽

2020 ◽

Vol 9 (3) ◽

pp. 451

Author(s):

Rizkah V. Mokoginta ◽

Herny E. I. Simbala ◽

Karlah L.R Mansauda

Keyword(s):

Antioxidant Activity ◽

Vitamin C ◽

Ethanol Extract ◽

Antioxidant Compounds ◽

Onion Plant ◽

Dpph Method ◽

Ic50 Values ◽

Experimental Laboratory ◽

Ethanol Extracts ◽

Eleutherine Americana

ABSTRACTDayak onion plant (Eleutherine americana Merr.) or commonly called forest onion is a typical plant of Central Kalimantan that has been used by local peoples for cure a various types of diseases. This study aims to determine the activity of antioxidant compounds from Dayak bulb ethanol extracts from Kotamobagu City. This research method is an experimental laboratory. Initially the Dayak bulb sample was extracted maceration with 96% ethanol solvent then evaporated until a thick extract was obtained. As a parameter, the DPPH method (1,1-diphenyl-2-picrylhydrazyl) was used to test the antioxidant activity of the samples at concentrations of 50, 60, 70, 80 and 90 mg / L and as a comparison standard used Vitamin C with concentrations of 2, 4, 6, 8 and 10 mg / L. The results obtained showed antioxidant activity in Dayak bulb ethanol extracts with IC50 values of 41.46 mg / L and on vitamin C IC50 values of 1.04 mg / L. Based on these results it can be concluded that the onion bulb ethanol extract has very strong antioxidant activity. Keywords: Dayak Onion bulbs, Activity, Antioxidant, DPPH ABSTRAK Tanaman bawang Dayak (Eleutherine americana Merr.) atau biasa disebut bawang hutan merupakan tanaman khas Kalimantan Tengah yang sudah dipergunakan masyarakat lokal sebagai obat berbagai jenis penyakit. Penelitian ini bertujuan untuk mengetahui aktivitas senyawa antioksidan dari ekstrak etanol bulbus bawang Dayak yang berasal dari Kota Kotamobagu. Metode penelitian ini adalah eksperimental laboratorium. Awalnya sampel bulbus bawang Dayak diekstraksi maserasi dengan pelarut etanol 96% kemudian dievaporasi hingga didapatkan ekstrak kental. Sebagai parameter, digunakan metode DPPH (1,1-diphenyl-2-picrylhydrazyl) untuk pengujian aktivitas antioksidan sampel pada konsentrasi 50, 60, 70, 80 dan 90 mg/L dan sebagai standar pembanding digunakan Vitamin C dengan konsentrasi 2, 4, 6, 8 dan 10 mg/L. Hasil penelitian yang didapat menunjukkan aktivitas antioksidan pada ekstrak etanol bulbus bawang Dayak dengan nilai IC50 sebesar 41,46 mg/L dan pada vitamin C nilai IC50 sebesar 1,04 mg/L. Berdasarkan hasil tersebut dapat disimpulkan bahwa ekstrak etanol bulbus bawang Dayak memiliki aktivitas antioksidan yang sangat kuat. Kata kunci: Bulbus Bawang Dayak, Aktivitas, Antioksidan, DPPH

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Antioxidant Studies of Thiazole Ring Bearing Chalcone Derivatives

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2021/v33i59a34280 ◽

2021 ◽

pp. 355-359

Author(s):

Ashok Babu Kasetti ◽

Jayesh Dwivedi ◽

Ravindra Nagasuri

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Antioxidant Activities ◽

Thiazole Ring ◽

Chalcone Derivatives ◽

Antioxidant Agents ◽

Ic50 Values ◽

The Common ◽

Common Problems

Oxidative stress is one of the common problems seen in a variety of diseases. Chalcones and in particular heteroaryl chalcones had reported with promising antioxidant activities. Hence, in the present work, we reported the antioxidant activity of twenty thiazole ring bearing chalcone derivatives (1-20). Among the tested compounds, compounds 17, 19 and 20 containing 2-pyridinyl, 3-pyridinyl and 2-thiazolyl scaffolds showed superior antioxidant activity than the standard with their IC50 values 4±1µg/mL, 3±1 µg/mL and 5±1 µg/mL respectively. The compound 19 is an interesting lead for the development of newer antioxidant agents.

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