scholarly journals CD10 expression in gastric carcinoma is correlated with tumor grade and survival

2019 ◽  
Vol 38 (1) ◽  
pp. 41
Author(s):  
Melika Kooshki Forooshani ◽  
Amir Hosein Jafarian ◽  
Leila Takallou ◽  
Nema Mohamadian Roshan
Keyword(s):  
Gastric Carcinoma ◽  
Stromal Cells ◽  
Clinical Stage ◽  
Tumor Grade ◽  
Tumor Stage ◽  
Lymph Node Involvement ◽  
Matrix Remodeling ◽  
Cross Sectional ◽  
Skin Malignancy ◽  
Cd10 Expression

Background<br />Gastric carcinoma (GC) is the most common non-skin malignancy in Iranian men and the second leading cause of cancer-related mortality. Invasion and metastasis are considered as the major causes of cancer-related morbidity and mortality. Proteinases such as matrix metalloproteinases play an important role in tumor progression and mediating extracellular matrix remodeling. CD10 is a 90-110kd cell surface zinc-dependent metalloproteinase and there is evidence that this membrane protein may facilitate invasion and/or metastasis of tumoral cells. The objective of this study was to determine the frequency of CD10 expression in the stromal cells of GC and determine its relationship with survival and clinicopathological factors. <br /><br />Methods<br />A cross-sectional study was performed involving 50 patients with histopathologic diagnosis of GC. CD10 expression was determined by immunohistochemistry (IHC). Survival of the patients as well as the grade and stage of the tumors and demographic variables were documented. The Kaplan-Meier test was used for data analysis.<br /><br />Results<br />Stromal CD10 was detected in 46% of the GC stromal cells. No immunoreactivity was identified in the stromal cells of normal adjacent tissue. Stromal CD10 expression in gastric carcinoma did not correlate with the age and gender of the cases as well as the size and location of the tumor, and lymph node involvement but correlated with tumor stage (p=0.01), tumor grade(p=0.01) and patients’ survival (p=0.02). <br /><br />Conclusion<br />Stromal CD10 expression is correlated with tumor differentiation, clinical stage and survival in GC. CD10 expression could be considered as a negative prognostic factor for gastric carcinoma.

scholarly journals Evaluation of clinical and laboratory characteristics of patients with bladder cancer in Ardabil province, 2016

2018 ◽  
Vol 5 (3) ◽  
pp. 885
Author(s):  
Ali Hoseinkhani ◽  
Firouz Amani ◽  
Yasamin Torabi
Keyword(s):  
Bladder Cancer ◽  
Urban Areas ◽  
Tumor Grade ◽  
Tumor Stage ◽  
Cross Sectional Study ◽  
Common Type ◽  
Blood Type ◽  
High Grade ◽  
Cross Sectional ◽  
Urethral Carcinoma

Background: Bladder cancer is the most common cancer of the urethra and genital tract and is ranked ninth in terms of its incidence. The aim of this study was to investigate the clinical and laboratory characteristics of patients with bladder cancer in Ardabil province.Methods: This is a cross-sectional study that has been done on 81 patients with bladder cancer. Necessary information such as age, sex, blood type, RH, family history of bladder cancer, smoking, drug use, tumor stage and grade and type of tumor were collected from the patient's hospital record or by telephone interview.Results: Sixty seven patients (82.7%) were male. The mean age of patients was 66.9±15.02 years. The blood group A, was the most common type of blood. (39.5%) of all patients, 54.3% had cigarette smoking and 47(58%) live in city. The most common type of tumor grade was high-grade papillary urethral carcinoma (48.1%).The most common stages of the tumor was Ta (40.7%) and the most common clinical manifestation was hematuria (90.1%). 27 patients (33.3%) had a delay of more than 3 months between observation of hematuria and cystoscopy.Conclusions: The findings showed that the most common grade and stage of the tumor in patients were high-grade papillary urethral carcinoma and Ta; the most prevalent clinical presentation was hematuria; the prevalence of the disease was higher among males, at ages older than 70 years old, in people with a blood type A, and among people living in urban areas; a study with bigger sample size should be done in future in the country Iran.

Grading as a Prognostic Factor: Review of 209 Primary Gastric Cancer Patients

1989 ◽  
Vol 75 (5) ◽  
pp. 494-497 ◽  
Author(s):  
Giovanni Battista Secco ◽  
Roberto Fardelli ◽  
Elisabetta Campora ◽  
Salvatore Rovida ◽  
Gabriella Lapertosa ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Lymph Node ◽  
Tumor Grade ◽  
Tumor Stage ◽  
Lymph Node Involvement ◽  
Primary Gastric Cancer ◽  
Node Involvement ◽  
Involvement Rate ◽  
Well Differentiated ◽  
Gastric Cancer Patients

Two hundred and nine cases of primary gastric cancer were treated surgically from January 1968 to December 1983 and analyzed retrospectively. All patients were followed up for a minimum of 5 years. There were 25 SI cases (12 %), 22 SII (10.5%), 55 SIII (26.3%) and 107 SIV (51.2%). Tumor grade according to Broders classification showed 50 cases of G1 lesions (23.9%), 44 G2 (21.1 %) and 115 G3 (55%). Patients with well differentiated G1 lesion, compared to G2-G3 patients, presented a greater incidence of T1-T2 tumors and decreased incidence of T4 tumors (p < 0.05). The lymph node involvement rate significantly increased with variation of T (p < 0.001) but not with tumor grade. Survival results correlated with tumor stage (p < 0.01) but not with tumor grade or histological type.

scholarly journals Clinical Evaluation of the Diagnostic Role of MicroRNA-155 in Breast Cancer

2020 ◽  
Vol 2020 ◽  
pp. 1-13
Author(s):  
Fatemeh Hosseini Mojahed ◽  
Amir Hossein Aalami ◽  
Vahid Pouresmaeil ◽  
Amir Amirabadi ◽  
Mahdi Qasemi Rad ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Rna Extraction ◽  
Tumor Grade ◽  
Breast Cancer Diagnosis ◽  
Tumor Stage ◽  
Lymph Node Involvement ◽  
Youden Index ◽  
Control Group ◽  
Roc Curve Analysis ◽  
Ki 67

Aim. Biochemical markers, including microRNAs (miRs), may facilitate the diagnosis and prognosis of breast cancer. This study was aimed at assessing serum miR-155 expression in patients with breast cancer and receptors. Methods. This case-control study was conducted on 36 patients with breast cancer and 36 healthy individuals. After RNA extraction from the patient’s serum, cDNA was synthesized. The expression of miR-155 was measured using RT-qPCR. Demographic and histochemical data were extracted from patient documents. Data were analyzed using the Statistical Package for the Social Sciences (SPSS) software. Results. The mean age of subjects in breast cancer and control groups was 47.64±8.19 and 47.36±7.52 years, respectively. The serum miR-155 expression was higher in the cancer group (1.68±0.66) compared to the control group (p<0.0001). There was a significant relationship between serum miR-155 expression and the tumor grade (p<0.001), tumor stage (p<0.001), and tumor size (p<0.001) of the patients. However, no relationship between miR-155 expression and the presence of lymph node involvement (p=0.15), HER2 (p=0.79), Ki-67 (p=0.9), progesterone receptor (p=0.54), and estrogen receptors (p=0.84) was found. The ROC curve analysis showed that the AUC was 0.89 (77.78% sensitivity and 88.89% specificity), and the cutoff was 1.4 (Youden index: 0.6667) for detecting breast cancer. Conclusion. The findings of this study revealed that serum miR-155 may serve as a potential noninvasive molecular biomarker for breast cancer diagnosis and can help predict the grade of the disease.

Baseline and longitudinal quantification of lean muscle mass using routine CT measurements prior to resection for pancreatic adenocarcinoma.

2019 ◽  
Vol 37 (4_suppl) ◽  
pp. 198-198
Author(s):  
Paul Eliezer Oberstein ◽  
Vilma Rosario ◽  
Xiaotao Guo ◽  
Binsheng Zhao ◽  
Jeanine M Genkinger ◽  
...  
Keyword(s):  
Neoadjuvant Chemotherapy ◽  
Muscle Mass ◽  
Locally Advanced ◽  
Neoadjuvant Treatment ◽  
Tumor Grade ◽  
High Volume ◽  
Tumor Stage ◽  
Significant Loss ◽  
Cross Sectional ◽  
Lean Muscle Mass

198 Background: When compared to healthy populations subjects are defined as sarcopenic if they have severe loss of lean muscle mass (LMM). Sarcopenia is associated with poor outcomes in some studies of pancreatic cancer (PDA) but the prevelance remains uncertain in resected patients and there is little data about changes in LMM during neoadjuvant chemotherapy. Muscle mass can be reliably quantified in cancer patients using routine imaging. We analyzed a large cohort of patients undergoing surgery at a high-volume center to quantify LMM at baseline and with treatment, and to determine the association of sarcopenia with tumor histology and outcomes. Methods: We analyzed subjects undergoing surgery for PDA at the Pancreas Center at Columbia University from 2011-2014. We utilized CT scans to measure cross sectional area of muscle at the L3 vertebral body (LMM in cm2) and used height to define a smooth muscle index (SMI-cm/m). Sarcopenia was determined based on SMI < 38.9 for females and < 55.4 for males. In subjects undergoing neoadjuvant chemotherapy, SMI was also calculated on post therapy scans. We collected information on clinical and pathological variables and performed statistical analyses utilizing SAS 9.4 software. Results: Among subjects with available imaging (n = 106) sarcopenia rates at initial staging were high whether they were immediately resectable (52%) or locally advanced (63%). Rates of sarcopenia were higher in males than females (77 vs 38%, p < 0.001). Sarcopenic status was not correlated with survival, tumor stage or grade but was associated with age, gender, and BMI. Among locally advanced subjects who proceeded to surgery after neoadjuvant chemotherapy (n = 40) there was no change in SMI or incidence of sarcopenia following treatment (median 171 days). Conclusions: Sarcopenia is highly prevalent at diagnosis in subjects undergoing surgery for PDA but was not associated with survival, tumor grade or stage in this cohort. Subjects who successfully completed neoadjuvant treatment did not experience significant loss in LMM despite extensive treatment suggesting that lack of change in LMM may assist in predicting favorable response to neoadjuvant therapy in PDA.

scholarly journals Clinicopathological, Immunohistochemical, and PMS2 Gene Expression Profiling of Patients with Sporadic Colorectal Cancer

2021 ◽  
Vol 24 (2) ◽  
pp. 86-93
Author(s):  
Maryam Mousavi ◽  
Mohammad Taghi Goodarzi ◽  
Seyed Mehrdad Kassaee ◽  
Ali Jafari Heidarloo ◽  
Mojtaba Fathi
Keyword(s):  
Colorectal Cancer ◽  
Clinical Stage ◽  
Tumor Grade ◽  
Tumor Stage ◽  
Pcr Analysis ◽  
Sporadic Colorectal Cancer ◽  
Mrna Levels ◽  
Dna Mismatch ◽  
Tissue Sections ◽  
Mutl Homolog 1

Background: The DNA mismatch repair (MMR) system is one of the molecular pathways involved in colorectal cancer (CRC) carcinogenesis that consists of several genes, including MLH1 (MutL homolog 1), MSH6 (MutS homolog 6), MSH2 (MutS homolog 2), and MSH3 (MutS homolog 3). The protein encoded by PMS2 (post-meiotic segregation 2) is also essential for MMR. Here, we address the correlation between immunohistochemical and transcriptional expression of PMS2 with the tumor grade and clinical stage of non-hereditary/sporadic CRC disease. Methods: This study retrospectively analyzed 67 colorectal resections performed for 38 male and 29 female patients. Random biopsies were taken by a gastroenterologist from patients referring to three hospitals in the cities of Zanjan, Urmia and Qazvin (Iran) during 2017-2019. All specimens were examined and classified for localization of tumor, pathological stage and grade. The PMS2 protein expression was studied immunohistochemically and analysis of mRNA expression was performed in the same tissue sections. Results: Immunohistochemistry and quantitative real-time polymerase chain reaction (PCR) analysis showed a decrease in PMS2 expression compared with paracancerous tissue (P<0.001), which correlated with tumor stage. In addition, reduced PMS2 expression was correlated with the tumor differentiation grade, underlining a connection between downregulation of PMS2 and progression of CRC. Comparing the PMS2 mRNA levels in different groups showed the following results: 0.92 ± 0.18 in patients with Stage I CRC tumor, 0.86 ± 0.38 in Stage Ⅱ, 0.50 ± 0.29 in Stage Ⅲ, and 0.47 ± 0.23 in Stage Ⅳ. Conclusion: These findings suggest that PMS2 may provide a potential reliable biomarker for CRC classification by combined immunohistochemical and mRNA analysis.

scholarly journals CD10 expression in gastric adenocarcinoma and its correlation with histopathological features and lymph node metastasis

2021 ◽  
Vol 12 (10) ◽  
pp. 75-80
Author(s):  
Bhavya Pappalamukalel Mohan ◽  
Sharath Kesavan Krishnan
Keyword(s):  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Gastric Carcinoma ◽  
Gastric Adenocarcinoma ◽  
Stromal Cells ◽  
Intestinal Morphology ◽  
Depth Of Invasion ◽  
Histologic Subtype ◽  
Node Metastasis ◽  
Cd10 Expression

Background: Gastric cancer is one of the most common cancers worldwide. Invasion and metastasis are known prognostic factors. Previous studies have suggested CD10 expression in the epithelium and stroma of various carcinomas is associated with more aggressive behavior of the tumor. Aims and Objective: To study the immunohistochemical expression of CD10 in stromal cells of gastric adenocarcinoma and to correlate the expression with various clinicopathological features and lymph node metastasis. Materials and Methods: A cross sectional study of CD10 expression in 40 cases of gastric carcinoma in gastrectomy specimens was done. CD10 expression was correlated with age, gender, tumor site, tumor grade, histologic sub type, depth of invasion and lymph node status. Results: Out of 40 cases, 72.5% were males and 27.5% were females. Majority of cases were seen in 6th and 7th decades. Antrum was the most common location (70%) and intestinal morphology was the commonest histologic subtype (47.5%). Tumor size ranged from 2.5 to 11cms. 40% tumors were well differentiated. Majority (62.5%) of the tumors were in T3 stage. 25 (62.5%) cases showed a positive CD10 expression in stromal cells. Stromal CD10 expression in gastric carcinoma did not correlate with the age and gender of the cases as well as the location and size of the tumor, histologic subtype and lymph node involvement but correlated with depth of invasion (T stage) (p <0.05). Conclusion: CD10 expression in gastric adenocarcinoma shows a significant correlation with depth of invasion. CD10 may be used as an immunohistochemical surrogate of tumor behavior.

Survival outcomes based on race in gastric carcinoma: A SEER database analysis.

2011 ◽  
Vol 29 (4_suppl) ◽  
pp. 5-5
Author(s):  
N. Didwaniya ◽  
R. J. Edmonds ◽  
P. T. Silberstein ◽  
S. Subbiah
Keyword(s):  
Gastric Cancer ◽  
Gastric Carcinoma ◽  
Survival Rates ◽  
Tumor Grade ◽  
The United States ◽  
Lymph Node Involvement ◽  
Tnm Staging ◽  
Treatment Modalities ◽  
Seer Database ◽  
Cancer Specific Survival

5 Background: Gastric cancer is one of the leading causes of cancer related deaths worldwide with the incidence declining in the United States. However the prognosis remains poor with variable survivals being reported among different races. We analyzed the effect of race on patterns of disease presentation and survival rates using the Surveillance, Epidemiology, and End Results (SEER) database. Methods: A total of 9,851 patients were diagnosed with gastric cancer from year 2004 to 2007 were identified from SEER database. Age, race, TNM staging, grade, treatment modalities utilized and cancer specific survival was collected. Results: Out of 9,851 patients, 64.63% were white, 12.17% were black, and 13.04% were Asian-Pacific islanders. Median age was 73 years for whites, 70 years in blacks, and 71 years in Asians. Sex distribution amongst races was more or less similar with 58.83% of whites, 59.47% of blacks, and 54.24% of Asians being men. 23.62% of whites had T1 lesions, 28.10% had T2, 19.58% were T3 and 28.70% had T4 lesions. 26.76% of blacks presented with T1 lesions, 26.63% with T2, 16.08% with T3 and 30.53% with T4. 18.69% of Asians had T1 lesions, 26.84% with T2, 23.44% had T3 and 31.03% had T4 lesions. 37.80% of whites, 36.70% of blacks, and 44.44% of Asians had lymph node involvement. Tumor grade was similar among all races. Surgery was performed in 31.49% of whites, 33.13% of blacks, and 40.48% of Asians. 14.68% of whites, 14.10% of blacks, and 19.43 % of all Asians underwent radiation therapy. Median overall survival in localized disease was 44 months, 43 months and 98 months (p < 0.0001) while in regional disease it was 16 months, 15 months and 23 months in whites, blacks and Asians respectively (p < 0.0001). Median survival in distant disease was 4 months in both whites and blacks; it was 5 months in Asians (p < 0.0001). Conclusions: Cancer-specific survival in gastric carcinoma is significantly better in localized, regional and metastatic disease in Asians when compared to whites and blacks independent of T stage, grade, nodal involvement and treatment modalities utilized. The reason for this observation is unclear, exposure and genetic factors are potential causes and this needs to be investigated. No significant financial relationships to disclose.

Immunohistochemical Expression of TGF-β3 in Oral Squamous Cell Carcinoma

2019 ◽  
Vol 10 (01) ◽  
Author(s):  
Asmaa Ali Hussein
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Transforming Growth Factor ◽  
Tumor Grade ◽  
Tumor Stage ◽  
High Rate ◽  
Female Ratio ◽  
Positive Expression

Squamous cell carcinoma characterized by poor prognosis due to aggressive tumor growth and dissemination high rate of tumor cell . age ranged of patient case included in the study 40-62 years and mean age 55±99. The sex distribution male/female ratio 1:1. Male case 15 and female 15 of the present study The results of clinical forums showed in the current study was endophytic 10(33.3%) in the same time Exophytic were presented in 20 cases (76.7%). Regarding distribution of the tumors site, the preponderance of them 19 cases 73.3% were located alveolar mucosa, followed by in the tongue 11 cases(36.7%) Tumor stage was analyzed and recorded in Oral squamous cell carcinoma included cases, the preponderance of them were Stage II 11 cases 36.7% followed by stage III 10 cases 33.3% , 9 cases 30.0% were stage I. While Concerning tumor grade, majority of them 15 cases 50% had grade II moderately differentiated SCC, while 11 cases 36.7% had grade III poorly differentiated SCC and 4 cases 13.3% had grade I well differentiated SCC Positive TGF-β3 immunostaining was detected as cell with staining brown color, all tissues sections included show Positive expression based on IHC teqnique. Positive Transforming Growth Factor TGF-β3 Immuno staining was found in all case results and display that 4 samples with percentage 13.3% expressed strong positive 87.67 ± 1.45 expression , 11cases 36.7% showed 51.33 ±0.88 positive expression moderate at the same time 15 samples 50.0% showed positive weak expression.

Qualitative anatomical restoration of the contact point of teeth – prevention of localized forms of periodontal diseases

2020 ◽  
Vol 25 (1) ◽  
pp. 10-15
Author(s):  
L. Yu. Orekhova ◽  
O. V. Prokhorova ◽  
V. Yu. Shefov
Keyword(s):  
Comparative Analysis ◽  
Computer Simulations ◽  
Periodontal Diseases ◽  
Contact Point ◽  
Clinical Stage ◽  
High Quality ◽  
Cross Sectional ◽  
Clinical Stages ◽  
Anatomical Restoration ◽  
Approximal Surfaces

Relevance. The restoration of a high-quality anatomical and functional contact point of teeth plays an important role in preventing the development of localized forms of periodontal disease.Purpose. Development of recommendations for qualitative anatomical restoration of the contact point of teeth for the prevention of localized forms of periodontal diseases.Materials and methods. In our study, which consisted of pre-clinical and clinical stages, were analyzed 50 CT scan of the chewing group teeth calculated the ratio of approximal surfaces of molars and premolars to the width of their crowns and computer simulations of the results of restoration of contact point. We also conducted a comparative analysis of wedges of different material with different cross-sectional shapes. At the clinical stage, the restoration of the contact point of teeth in patients according to our recommendations was carried out.Results. On the basis of the data obtained during the study, a formula for calculating the height and size of the restored contact point was compiled. Recommendations for anatomical restoration of the contact point are formulated.Conclusion. The application of the recommendations developed by us allows anatomically qualitatively restore the contact point and prevent the development of localized periodontal diseases.

Opportunistic infections among people living with HIV at reference health care centers in Morocco, 2015 (Preprint)

2019 ◽  
Author(s):  
Noureddine Sakhri ◽  
Fatima Zahra Meski ◽  
SOUMIA TRIKI
Keyword(s):  
Health Care ◽  
Opportunistic Infections ◽  
Oral Candidiasis ◽  
Clinical Stage ◽  
Bivariate Analysis ◽  
People Living With Hiv ◽  
Cross Sectional Survey ◽  
Pneumocystis Jiroveci ◽  
Cross Sectional ◽  
Health Care Centers

BACKGROUND Morbidity and mortality in HIV disease is due to immune-suppression leading to life-threatening opportunistic infections (OIs) during the natural course of the disease. In 2015, the HIV prevalence is low in general population and concentrated among key populations. OBJECTIVE This study aimed to assess the prevalence and CD4 correlates of OIs among adult HIV-infected patients attending antiretroviral health care in Morocco, during 2015. METHODS We conducted a cross-sectional survey among all adult PLHIV for admitted in the health care centers during 2015, who had acquired infection disease. Patients’ opportunistic infection status was determined through clinical diagnosis and laboratory investigations. CD4 count was determined using flow cytometry technique. The clinical stage of HIV was identified by the classification of Centers for Disease Control and Prevention (CDC). We collected Socio-demographic and clinical data from patients’ medical records. We performed statistical analysis by using Epi-Info 7.2.0.1 software. The appropriate test was applied, bivariate analysis was made and the differences were significant when p<.05. RESULTS 299 HIV-infected cases were included; 53% were males. The most represented age group was 25-34 years (36.1%). The mean age of the cases was 38.7 ± 16.8. The prevalence of OIs was 47.8%. Tuberculosis (65/299, 21.7%), Pneumocystis jiroveci pneumonia (40/299, 13.4%) and Oral candidiasis (22/299, 7.4%) were the most frequently observed OIs. CONCLUSIONS Tuberculosis, pneumocystis and oral candidacies were the leading OIs, encountered by HIV-infected cases. Preventive measures and early diagnosis of HIV associated to OIs are crucial.

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