scholarly journals Parameters of the Histological Adaptation of Marmorkrebs Procambarus virginalis (Lyko, 2017) (Decapoda, Cambaridae) to Manganese, Nickel and Lead Ions Pollution

2018 ◽  
Vol 70 ◽  
pp. 24-33 ◽  
Author(s):  
Alexandra Naboka ◽  
Oleg Marenkov ◽  
Julia Kovalchuk ◽  
Zoya Shapovalenko ◽  
Oleh Nesterenko ◽  
...  

Manganese, lead and nickel are heavy metals, which are the priority fresh water toxic contaminants and relate to of the first danger class chemicals. Heavy metals cause functional disorders in the tissues and organs of hydrobionts, affecting their linear and weight indices, reproductive system, digestive and extraction organs. In the experiment on marbled crayfishProcambarus virginalis(Lyko, 2017) studied the long-term effects of various concentrations of heavy metal ions on the physiological state and histostructure of tissues and organs. According to the results of research, it was found that among the studied heavy metals nickel ions influenced the weight indexes and mortality of crustaceans the most negatively. The results of morphometric studies on marbled crayfish indicate no significant changes in the control group and experimental one, but heavy metals influenced the form of the hepatopancerase lobes and the size of the lumen of the gland. Research on adipocytes of the connective tissue showed a significant difference between the size of cells under the influence of heavy metals. Dimensions of adipocytes fluctuated in a wide range from 144 μm2to 537 μm2. In control group the adipocyte area was 406.96 μm2. Experimental studies of hemolymph showed that, under the influence of lead ions, a significant increase in 1.4 times was observed in the area of hemolymph cells; when manganese and nickel were added, moderate decrease was observed in cells. It was found that under the influence of nickel, the area of round hyalinocytes has reduced by 1.7 times.

2018 ◽  
Vol 70 ◽  
pp. 11-23 ◽  
Author(s):  
Oleg Marenkov ◽  
Mykola V. Prychepa ◽  
Julia Kovalchuk

In the experiment with marbled crayfishProcambarusvirginalis(Lyko, 2017), chronic effects of various concentrations of heavy metal ions on the physiological state and enzyme activity were investigated. The obtained results showed that among the investigated heavy metals nickel ions influenced the weight indexes and mortality of crustaceans the most negatively. According to the results of the research, significant changes were noted in the individual biochemical parameters of marbled crayfish under the influence of manganese, lead and nickel ions. The most significant changes in the activity of lactate dehydrogenase were detected in muscle tissues affected by manganese and nickel ions. A significant decrease in the activity of succinate dehydrogenase in muscle of marbled crayfish was determined after the action of heavy metal ions. Investigation of changes in the activity of alkaline phosphatase under the influence of the ions of manganese, lead and nickel has its own characteristics, which indicates certain violations in the tissues of cell membranes. Changes in the activity of enzymes were also reflected in the overall protein content. Changes in these parameters may indicate a rapid biochemical response of crustaceans to the toxic effects of heavy metals.


Author(s):  
Santosh Kumar

Background: Midface symmetry is an important indicator of success of complete unilateral cleft lip and palate (CUCLP) treatment. There is little literature on the long-term effects of Presurgical Nasoalveolar Molding (PNAM) on Midface symmetry in children treated for CUCLP. Material and Methods: This case-control study was carried out in the Department of Burn and Plastic Surgery at Government Medical College and Hospital, Bettiah, Bihar, India from may 2018 to Dec 2019. Complete unilateral CL+P patients had basilar and frontal photographs at two time points: (1) initial (2) postsurgical. 30 nasal molding patients and 20 control patients were included. Presurgical nasal molding was performed prior to primary lip repair in intervention group. No nasal molding was performed in control group. Results: A statistically significant difference was found for postsurgical nostril height-width ratio (P< .05). No other statistically significant differences were found. Conclusion: Nasal molding and surgery resulted in more symmetrical nostril height-width ratios than surgery alone. Alar groove ratios were not statistically significantly different between groups perhaps because application of nasal molding was not early enough; postsurgical nasal splints were not utilized; overcorrection was not performed for nasal molding


1986 ◽  
Vol 6 (6) ◽  
pp. 724-738 ◽  
Author(s):  
A. C. Evans ◽  
M. Diksic ◽  
Y. L. Yamamoto ◽  
A. Kato ◽  
A. Dagher ◽  
...  

Regional cerebral blood volume (CBV) can be calculated using data obtained during the kinetic analysis of 18F-labeled 2-fluoro-2-deoxy-d-glucose (FDG) uptake measured by positron emission tomography (PET). As a result the influence of vascular activity upon the determination of FDG rate constants can be minimized. The method is investigated by simulation experiments and by analysis of PET studies on seven older, healthy human volunteers aged 52–70 years. The accuracy of measured FDG rate constants k1, k2, and k3, obtained either by omitting the early portion of the uptake curve or by explicit inclusion of CBV as a fit parameter, is compared. The root mean square error in measured rate constant for the latter method is equivalent to that obtained by omitting the first 2.5–3 min of tissue data and neglecting the CBV term. Hence, added information about the physiological state of the tissue is obtained without compromising the accuracy of the (FDG) rate constant measurement. In hyperemic tissue the explicit determination of the vascular fraction results in more accurate estimates of the FDG rate constants. The ratio of CBV determined by this method to CBV obtained using C15O in six subjects with CBV in the normal range was 0.92 ± 0.32. A comparison of the CBV image obtained by this method with that obtained using C15O in an arteriovenous malformation case demonstrates the accuracy of the approach over a wide range of CBV values. The mean value for CBV fraction in gray matter obtained by this method in the older control group was 0.040 ± 0.014. Average gray matter rate constants obtained were k1 = 0.084 ± 0.012, k2 = 0.150 ± 0.071, and k3 = 0.099 ± 0.045 min−1.


2006 ◽  
Vol 21 (8) ◽  
pp. 551-562 ◽  
Author(s):  
Thomas A. Pagonis ◽  
Nikiforos V. Angelopoulos ◽  
George N. Koukoulis ◽  
Christos S. Hadjichristodoulou

AbstractObjectiveThe objective of our study was to evaluate the psychological consequences of real-world AAS use in athletes abusing such agents, in comparison with a placebo and control group of comparable athletes, while correlating the severity of abuse with the side effects observed. The hypothesis tested by the study was that the use of AAS induces a wide range of psychological side effects whose impact and emergence is dependent upon the severity of the abuse.DesignThe study includes a substantial group of AAS abusing athletes and two more groups demographically similar to the first, one composed of athletes not using any substance and a placebo group. All athletes were stratified according to the severity of AAS abuse. Psychometric instruments were applied to all athletes in specific time intervals, dependent to the AAS abusers' regimens, providing us with a final psychological profile that was to be compared to the pre-study profile. All results were comparable (within and between groups) for statistically significant differences and correlated to the severity of the abuse. Homogeneity of all groups was safeguarded by random doping controls, monitoring of drug levels and analysis of all self obtained drugs by method of liquid chromatography/mass spectrometry. All athletes were provided with a common exercise and dietary regime, so common training and nutritional conditions were achieved.MethodsWe studied a cohort of 320 body-building, amateur and recreational athletes, of whom 160 were active users of AAS (group C), 80 users administering placebo drugs (group B) and 80 not abusing any substance (Group A). Group C athletes were stratified according to AAS abuse parameters, thus providing us with three subgroups of “light, medium and heavy abuse”. Athletes of groups A and B were included in a “no abuse” subgroup. The psychometric instruments used were the Symptoms Check List-90 (SCL-90) and the Hostility and Direction of Hostility Questionnaire (HDHQ). The psychometric evaluations took place within a time interval of 13 months. Statistical analysis was performed by using the Mann–Whitney/Wilcoxon two-sample non-parametric test (Kruskal–Wallis test for two groups) for data that were not normally distributed and Linear regression analysis was used to ascertain the correlation between severity of use and escalation of side effects.ResultsThe study showed a statistically significant increase in all psychometric subscales recorded in group C, and no statistically significant difference in group C and A. There was a significant increase in the scorings of group C for all subscales of SCL-90 and HDHQ. Correlation of abuse severity and side effects showed that there was a statistical significant increase in Δ values of all SCL-90 and HDHQ subscales that escalated from light abuse to medium and heavy abuse/consumption patterns.ConclusionsThe results of the study suggest that the wide range of psychiatric side effects induced by the use of AAS is correlated to the severity of abuse and the force of these side effects intensifies as the abuse escalates.


2020 ◽  
Vol 222 ◽  
pp. 02014
Author(s):  
Lidia Kashirina ◽  
Konstantin Ivanischev ◽  
Kirill Romanov

The article presents the results of experimental studies to determine the effect of antioxidant drugs “Butofan” and “E-selenium” on veterinary and sanitary parameters of cows’ milk obtained in the period after calving. Childbirth is characterized by a stressful physiological state of the body and a large amount of lipid peroxidation (LPO) products are formed in the blood of cows, which have a negative depleting effect on the body and, naturally, on the quality of milk, since it is a blood product. To enhance the work of the body’s own antioxidant system, since it is not always enough to neutralize LPO products, antioxidant drugs are used. It is necessary to determine veterinary and sanitary parameters in milk obtained under the influence of any drugs, since it is used in human nutrition. For this purpose, experimental studies were carried out on analogous dairy cows in one of the farms of Ryazan region. The cows were divided into three groups: the control and two experimental ones. The control group of animals was intact, the cows of the experimental groups received antioxidant preparations: the first one got “E-selenium” and the second one got “Butofan”. The research results showed that the milk yield of cows in the experimental groups was higher compared to the control. The quality parameters of milk in the experimental groups of cows were better in terms of fat content, protein content, density and acidity. Cow’s milk under the influence of antioxidant drugs was biologically complete and environmentally friendly.


2021 ◽  
Vol 14 (3) ◽  
pp. 1587-1593
Author(s):  
Emad K. Abbas ◽  
Hussein H. Echrish ◽  
Sabaa A. Mohammed

Background:Turmeric is typically used as a spicy food preservative and colorant. It has been proved that curcumin has a wide range of biological effects including anti-inflammatory, anti-viral, anti-fungal, and curcumin activity that can improve antibiotic activity on the wounds. Objectives: To evaluate the effects of Curcumin with and without antibiotics on skin wound treatment. Materials and Methods: The protocol was approved by the animal house in medical college / Basra university. This study used nine male rabbits aged about 6 months and an average weight of (1.083 g). Each group consists of 3 rabbits: control group (normal saline) A, topical curcumin in group B, topical curcumin, and tetracycline ointment in group C. Regular treatments were given to rabbits in therapeutic groups. Result: The lowest Mean ± SDof swelling of suturing area was noted in both groups that treated by curcumin alone (9.07 ± 0.97 vs 15±1 mm, p value = 0.002) and that treated with curcumine and antibiotic (9.1±0.9vs 15±1 mm, p value = 0.002) versus the control group ( that treated by normal saline) and the lowest Mean ± SD of elevation of suture line was noted in both group that treated by curcumin alone (2.63 ± 0.06 vs 4.07±0.21 mm, p value >0.001) and that treated with curcumin and antibiotic (2.7 ± 0.2 vs 4.07±0.21 mm, p value =0.001) versus control group. There is no significant statistical difference between the Mean ± SD neither of swelling of suture area nor of elevation of suture lines of groups that treated by curcumin alone and group that treated with curcumin and antibiotic [(9.07 ± 0.97 vs 9.1 ±0.9, p value=0.97),(2.63±0.06 vs 2.7 ± 0.2, p value=0.61) respectively]. The histopathological evaluation is consistent with morphological changes as at day 3 of wound healing in both groups that treated by curcumine with and without antibiotic, there is formation a thin layer of keratin and absence of features that indicate delay wound healing such as hemorrhage, inflammatory cell infiltrate of (Neutrophils, macrophages and lymphocytes) and debris, which are detected in control group. Furthermore, at day 7 of control group, there is decrease of inflammation, presence of gap between the two edgesof the wound but no keratin formation. No clear histopathological difference in wound healing between tested groups that treated by curcumin with and without antibiotic. Conclusion: There issignificant clinical and histological evidences that the curcumin not only prevent delay of wound healing but it is also enhanced wound healing. No significant difference in using curcumin alone or combine it with local antibiotic.


Author(s):  
O. Malyk ◽  
Ya. Protsyk ◽  
N. Shkodyak ◽  
T. Yurynets ◽  
S. Martynyk ◽  
...  

Sodium-containing preparations have antimicrobial, anti-inflammatory and wound-healing activity, which determines their use in veterinary medicine. This effect is due to a wide range of physiological effects of sodium ions on animals metabolism. The article presents the results of experimental studies of the irritant effect of sodium-containing drugs: known – 2 % glycetinate, its analogues – 2 % ovocid, 3 % ovocid, 1.5 % potassium-sodium drug (Na / K, 1.5%). Studies of irritant and skin-resorptive action of drugs were performed by immersing the tail of animals in a test tube with the substance on white rats weighing 250–270 g. Four groups of rats were formed with 3 animals each: rats of I group (control) tails were immersed in test tubes with 2 % glycetinate, animals of II group – in test tubes with 2% oocide, III group – in test tubes with 3 % oocide, and in IV group rats – with Na / K, 1.5% drug. The exposure time was 4 hours. The animals were subjected to clinical observations, assessing their general condition and the reaction of the tail’s skin. The effect of drugs on the morphological and biochemical parameters of the rats blood was studied. The study of the local irritant effect of the studied drugs in rats from I, II and III groups showed a moderate local irritant reaction, as evidenced by the appearance of redness and thickening of the skin, and in group IV animals appeared only slight redness on the skin of the tails. This indicates a weaker local irritant effect of potassium-sodium-containing drug (1.5% K, Na), compared with other studied drugs. According to hematological studies, in the rats’s blood of groups III and IV, a reliable increase, within normal limits, the number of leukocytes and a decrease in hemoglobin content, compared with those of animals of group I. It was found that at 4 hours of the experiment under the action of the studied potassium-sodium drugs increases the permeability of Sodium and Potassium through the rats’s skin into the blood. In particular, in serum of animals from groups II and IV there was a reliable increase of Potassium content by 2.1 times, respectively, and a significant increase in Sodium content in the blood of animals from groups III and IV, compared with control group. In animals of groups II and IV there was a reliable increase of serum aspartate aminotransferase activity, and in rats of group III – increase of serum alanine aminotransferase, alkaline phosphatase activity and in triacylglycerol’s content, compared with rats of group I, which indicates the effect of 2 % glycecin analogues on metabolic processes in animals.


2021 ◽  
pp. 110-118
Author(s):  
Tetiana Fotina ◽  
Hanna Fotina ◽  
Svitlana Nazarenko ◽  
Roman Tymoshenko ◽  
Oleksii Fotin

The results of the effect of feeding the chelated form of Zinc on the safety, productivity and slaughter parameters of broiler chickens are presented. The aim of this study was to determine the safety, productivity and slaughter parameters of broilers on the background of feeding chelated form of Zinc. Materials and methods. Experimental studies were conducted during 2020 on broiler chickens cross Cobb-500 in the vivarium of the Veterinary Faculty of Sumy National Agrarian University (Ukraine), divided into two groups on the principle of analogues (control and experimental) of 50 broilers each. The source of zinc for chickens in the control group was their sulfates. Chelated forms of Zinc were used for broiler chickens of the experimental group. All groups of broiler chickens were administered the compound with compound feed in a dose that corresponded to the daily requirement of the bird in this trace element. The duration of the experiment is 42 days. The growth and development of broilers were evaluated on the basis of determining the relevant zootechnical indicators. Live weight of birds was determined by individual weighing on scales of the VNC type with an accuracy of ±1 g at the age of 7, 14, 21, 28, 35 and 42 days. Both scientific and economic experiments also determined the preservation of livestock - daily by the number of culled and dead birds. Feed consumption was recorded daily, for each week of cultivation and for the entire period of the experiment. At the end of the experiment, the cost of feed per 1 kg of live gained weight was calculated. Results. According to the results of the conducted researches, it was established that feeding of the chelated form of Zinc probably increased both the average daily gain and the total live weight of broiler chickens at different age periods of rearing. The live weight of chickens at the beginning of the experiment was almost the same, but at 7 days of age it tended to increase in chickens of the experimental group, although a significant difference between groups in this indicator was not found. It was found that the weight of uneviscerated, semi-eviscerated and eviscerated chicken in poultry of the experimental group was greater than similar indicators in the young animals of the control group by 9.3, 9.2, and 9.8 %, and this difference was significant (p<0.01). The yield of semi-eviscerated carcasses was higher in ones of the experimental group (84.19 %), and the difference was significant (p<0.05). Conclusions. It was found that the live weight of chickens of the experimental group (consumption of feed with a chelated form of Zinc) prevailed at 7 days of age by 1.1 g; on the 14th day – on 22.1 g (p<0.01); at 21 days – 35.4 g (p<0.01); at 28 days – 94.5 g (p<0.05); at 35 days – 133.6 g (p<0.01); at 42 days – at 218.9 g (p<0.05). Preservation of young animals in the experimental group, which used chelated compounds of Zinc, was higher and amounted to 96 %, compared with the control – 92 %. By age, feed costs per unit weight gain were lower in the experiment, respectively: 0.05; 0.08; 0.12; 0.06; 0.07 and 0.05 kg, compared to the control group. Growing broiler chickens on compound feed with a chelated form of Zinc by 9.8 % (p<0.01) increases the weight of the eviscerated carcass.


Author(s):  
Mohammad Reza Zarei ◽  
Sara Bagheri ◽  
Amin Sedigh ◽  
Mohammad Ghasembandi

Background & Aim: A wide range of clinical education of operating room students is done in the operating room. One of the problems in students' clinical education is the lack of appropriate learning tools in the operating room. The use of educational tools that improve students' performance affects students' self-efficacy in the operating room. So, the purpose of this study was to investigate the influence of the surgical preference card as an educational aid tool on the self-efficacy of the operating room students. Methods & Materials: This quasi-experimental study was carried out on 64 operating room students of Isfahan University of Medical Sciences at AL-Zahra Hospital in the year 2018. Participates were selected through convenience sampling and were divided into experimental (n=32) and control (n=32) groups. The students in the intervention group performed surgical care with using the surgical preference card. The data collection tool was a clinical self-efficacy questionnaire. Data were analyzed with SPSS-21 software using the independent-t, paired t-test, and chi-square test. Results: The findings of this study showed that there was no significant difference between the average self-efficacy score of the control group before and after the intervention (p>0.05). while the average self-efficacy score of the experimental group increased significantly after the intervention as compared with before the intervention (p <0.001). Conclusion: The surgical preference card as an educational aid tool improved the students' selfefficacy. Therefore, it is recommended to use this tool for the clinical education of other operating room students.


2018 ◽  
Vol 20 (83) ◽  
pp. 226-229
Author(s):  
T.I. Fotina ◽  
H.A. Fotina ◽  
I. V. Kovalenko

The most vulnerable place in poultry farms is incubation, microorganisms are able to survive the entire period of incubation and penetrate through the eggshell, to be the source of infection with embryos, to affect the reduction of the percentage of conditional young birds, growth and development in the early days of cultivation. The accumulated pathogenic microflora negatively affects the state of the immune system of the bird. Contaminated with conditionally pathogenic and pathogenic microflora egg is the main link in the transmission of all bacterial infections. In Ukraine, for the pre-incubation treatment of eggs, sodium hydroxide, formalin, chloramine, persicum C. are used. These agents exhibit bactericidal properties, but are toxic, have corrosive activity and are carcinogens. The «SunTime» kit consists of polyhexamethylene guanidine hydrochloride and a mixture of organic acid salts (lemon, amber, and apple). These substances provide an effective bactericidal action against a wide range of bacteria, are safe for poultry embryos and personnel, have a stimulating effect on the growth and development of the embryo, provide a prolonged bactericidal action. The purpose of the study of the effectiveness of a complex ecologically safe sanitizers set «SunSim», its application for pre-incubation processing of chicken eggs. In experimental studies, two groups were formed, each with n = 250. In order to disinfect the surface of the shell of the incubation egg, two times aerosol treatment of the experimental group using 0.3% of SunStim was carried out, the first time the sanitation of the incubation eggs was carried out before the laying on the incubation cabinet, the exposure was 40 minutes, the second final treatment was carried out on the 18th day of incubation, to extract, a working solution of organic acids (amber, lemon and apple) to stimulate embryonic viability and increase the natural resistance of the embryos. For the control group, formalin was used. After two times of the sanation of the incubation eggs with the SunStim set, the percentage of decontamination of the shell surface of the incubation eggs was 99.1 ± 0.5%. While analyzing the results of the incubation waste category of «dead and drowning» by 3,6% less compared with the control, and the category of «crippled» was not observed. The use of the SunStim set improves the quality of surface treatment of the shell of incubation eggs, increases the incubation eggs output by 4.5-5.0%, the average body weight of the received young animals in experimental groups exceeds control by 4%.


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