scholarly journals STUDY OF IRRITANT AND SKIN-RESORPTIVE ACTION OF SODIUM-CONTAINING DRUG

2020 ◽  
Vol 21 (2) ◽  
pp. 105-109
Author(s):  
O. Malyk ◽  
Ya. Protsyk ◽  
N. Shkodyak ◽  
T. Yurynets ◽  
S. Martynyk ◽  
...  
Keyword(s):  
Experimental Studies ◽  
Sodium Content ◽  
Test Tube ◽  
Control Group ◽  
Potassium Sodium ◽  
Normal Limits ◽  
White Rats ◽  
Group I ◽  
Wide Range ◽  
Reliable Increase

Sodium-containing preparations have antimicrobial, anti-inflammatory and wound-healing activity, which determines their use in veterinary medicine. This effect is due to a wide range of physiological effects of sodium ions on animals metabolism. The article presents the results of experimental studies of the irritant effect of sodium-containing drugs: known – 2 % glycetinate, its analogues – 2 % ovocid, 3 % ovocid, 1.5 % potassium-sodium drug (Na / K, 1.5%). Studies of irritant and skin-resorptive action of drugs were performed by immersing the tail of animals in a test tube with the substance on white rats weighing 250–270 g. Four groups of rats were formed with 3 animals each: rats of I group (control) tails were immersed in test tubes with 2 % glycetinate, animals of II group – in test tubes with 2% oocide, III group – in test tubes with 3 % oocide, and in IV group rats – with Na / K, 1.5% drug. The exposure time was 4 hours. The animals were subjected to clinical observations, assessing their general condition and the reaction of the tail’s skin. The effect of drugs on the morphological and biochemical parameters of the rats blood was studied. The study of the local irritant effect of the studied drugs in rats from I, II and III groups showed a moderate local irritant reaction, as evidenced by the appearance of redness and thickening of the skin, and in group IV animals appeared only slight redness on the skin of the tails. This indicates a weaker local irritant effect of potassium-sodium-containing drug (1.5% K, Na), compared with other studied drugs. According to hematological studies, in the rats’s blood of groups III and IV, a reliable increase, within normal limits, the number of leukocytes and a decrease in hemoglobin content, compared with those of animals of group I. It was found that at 4 hours of the experiment under the action of the studied potassium-sodium drugs increases the permeability of Sodium and Potassium through the rats’s skin into the blood. In particular, in serum of animals from groups II and IV there was a reliable increase of Potassium content by 2.1 times, respectively, and a significant increase in Sodium content in the blood of animals from groups III and IV, compared with control group. In animals of groups II and IV there was a reliable increase of serum aspartate aminotransferase activity, and in rats of group III – increase of serum alanine aminotransferase, alkaline phosphatase activity and in triacylglycerol’s content, compared with rats of group I, which indicates the effect of 2 % glycecin analogues on metabolic processes in animals.

New and safe experimental model of radiation-induced neurovascular histological changes for microsurgical research

2016 ◽  
Vol 51 (2) ◽  
pp. 124-137
Author(s):  
Sergi Barrera-Ochoa ◽  
Irene Gallardo-Calero ◽  
Andrea Sallent ◽  
Alba López-Fernández ◽  
Ramona Vergés ◽  
...  
Keyword(s):  
Side Effects ◽  
Experimental Model ◽  
Experimental Studies ◽  
Neurovascular Bundle ◽  
Control Group ◽  
Sprague Dawley ◽  
Histological Changes ◽  
Group I ◽  
Radiation Induced

The aim is to create a new and safe experimental model of radiation-induced neurovascular histological changes with reduced morbidity and mortality for use with experimental microsurgical techniques. Seventy-two Sprague–Dawley rats (250–300 g) were divided as follows: Group I: control group, 24 rats clinically evaluated during six weeks; Group II: evaluation of acute side-effects (two-week follow-up period), 24 irradiated (20 Gy) rats; and Group III: evaluation of subacute side-effects (six-week follow-up period), 24 irradiated (20 Gy) rats. Variables included clinical assessments, weight, vascular permeability (arterial and venous), mortality and histological studies. No significant differences were observed between groups with respect to the variables studied. Significant differences were observed between groups I vs II–III regarding survival rates and histological changes to arteries, veins and nerves. Rat body weights showed progressive increases in all groups, and the mortality rate of the present model is 10.4% compared with 30–40% in the previous models. In conclusion, the designed model induces selective changes by radiotherapy in the neurovascular bundle without histological changes affecting the surrounding tissues. This model allows therapeutic experimental studies to be conducted, including the viability of microvascular and microneural sutures post radiotherapy in the cervical neurovascular bundle.

TOTAL FLAVONOID DAN EFEKTIVITAS EKSTRAK ETANOL BIJI KELOR (Moringa oleifera L) ASAL KOTA PALU SULAWESI TENGAH TERHADAP HISTOPATOLOGI PANKREAS TIKUS PUTIH JANTAN (Rattus norvegicus) YANG DIINDUKSI STREPTOZOTOCIN

2020 ◽  
Vol 6 (1) ◽  
pp. 24
Author(s):  
Viani Anggi ◽  
Joni Tandi ◽  
Veronika Veronika
Keyword(s):  
Ethanol Extract ◽  
Negative Control ◽  
Total Flavonoid ◽  
Male Rats ◽  
Control Group ◽  
Β Cells ◽  
Pancreatic Β Cells ◽  
White Rats ◽  
Group I ◽  
Streptozotocin Induced Diabetes

This study aims to determine the content of flavonoid and the effect of ethanol extract of moringa seeds on the regeneration of pancreatic β cells in male white rats streptozotocin induced diabetes. This study method used has total flavonoid equivalent quercetin by spectrophotometry uv-vis and to regeneration of pancreatic β cells in male white rats used 30 test animals,namely male white rats divided into 6 groups, each group consisted of 5 male white rats with details of group I as normal control, Group II as negative control given 0.5% Na-CMC suspension, Group III as positive control given glibenclamide suspension and in Groups IV, V, and VI were given with each dose of 100 mg/kg BW, 200 mg/kg BW and 400 mg/kg BB. Histopathological damage picture of the pancreas was observed by staining HE using a 400x magnification olympus Cx21 microscope. The results showed that the ethanol extract of moringa seeds contained secondary metabolites, namely flavonoids, alkaloids, saponins and tannins. The results showed has total flavonoid equivalent quercetin of moringa seeds is 1,26% and regeneration of pancreatic β cells in male white rats streptozotocin induced diabetes of Moringa seed ethanol extract at a dose of 400 mg/kg BB can have an effect on the regeneration of β cells in the pancreas of white diabetic male rats.  

The Effect of Ethanol Extract of Guava Leaf on Hypercholesterolemia-Diabetes of White Rats Male Induced by High Fat Feed and Streptozotocin

2021 ◽  
Vol 7 (2) ◽  
pp. 177-187
Author(s):  
Joni Tandi ◽  
Franciska Diana Pratiwi ◽  
Surisna Surisna ◽  
Niluh Puspita Dewi ◽  
Magfirah Magfirah
Keyword(s):  
Blood Glucose ◽  
Effective Dose ◽  
Ethanol Extract ◽  
Negative Control ◽  
Control Group ◽  
Glucose Levels ◽  
White Rats ◽  
Blood Glucose Levels ◽  
Group I ◽  
Guava Leaf

This study aims to determine the effect of guava leaf extract (Psidiium guajava L) on hypercholesterolemia-diabetes and the effective dose for reducing cholesterol and blood glucose levels. This study is a laboratory experimental study using 30 rats divided into 6 test animals. the treatment group, each group consisted of 5 rats. Group I was normal control, group II negative control was given Na-CMC suspension, group III positive control was given simvastatin group IV dose 150 mg / kg BW, group V dose 250 mg / kg BW and group VI dose 350 mg / kg BW. The results showed that the ethanol extract of guava leaves had an effect on reducing blood glucose levels of male white rats with an effective dose of 250mg / kg BW with an average reduction of 119 mg/dL and effectively reduce cholesterol levels at a dose of 150 mg /kg BW with an average reduction of 28,33 mg/dL.

scholarly journals EFFECTIVENESS OF LEAF EXTRACT REDUCE INFLAMMATION REACTION IN HYPERCHOLESTEROLEMIA RATS

2017 ◽  
Vol 1 (1) ◽  
pp. 12
Author(s):  
Puguh Santoso ◽  
Ni Nyoman Wahyu Udayani ◽  
I Nyoman Gede Tri Sutrisna ◽  
Ketut Agus Adrianta
Keyword(s):  
Free Radicals ◽  
Leaf Extract ◽  
Oxidized Ldl ◽  
Oxidative Modification ◽  
Blood Cholesterol ◽  
Ldl Oxidation ◽  
Control Group ◽  
Control Group Design ◽  
White Rats ◽  
Group I

Abstract High blood cholesterol is often called hypercholesterolemia is a risk factor for the emergence of pathological conditions such as heart and blood vessel disease. Hypercholesterolemia has an important role in the occurrence of damage to the endothelial cells is mainly caused by oxidized LDL. Oxidation of LDL triggers the formation of TNF - ?. Leaves messengers that allegedly contains flavonoids can improve the situation of hypercholesterolemia through the barriers specifically the expression of TNF - ? increased due to hypercholesterolemia. Plants messengers known to contain alkaloids, flavonoids, tannins, saponins, polyphenols, calcium oxalate, fats, and essential oil. Flavonoids which has the ability to bind the atom to form free radicals not to excess free radicals, thereby inhibiting the oxidative modification of LDL become ox-LDL so it will not be formed atherosclerosis. This study uses the Randomize pattern Pre and Post Test Control Group Design, using white rats (Rattusnovergicus) with Wistar strain aged 3-4 months, weighing 175-200 grams. Divided into four groups: Group I as a control with placebo, Group II treatment using extracts of ethanol leaves a messenger at a concentration of 10%, Group III treatments using extracts of ethanol leaves a messenger at a concentration of 20%, Group IV is to use the extract ethanol leaves errand at a concentration of 30%. It can be concluded at 4 dose group 30% messengers leaf extract significantly different, p <0.05, so it can be said that the provision of effective messengers extract at a dose of 30%.

scholarly journals The Effect of Avocado to the Profile of Blood Urea Nitrogen (BUN) and Creatinine in Rats (Rattus norvegicus) Induced with Meloxicam

2018 ◽  
Vol 2 (1) ◽  
Author(s):  
Amelia Ramadhani Anshar ◽  
Muhammad Akbar Bahar ◽  
Dini Kurnia Ikliptikawati
Keyword(s):  
Treatment Group ◽  
Creatinine Level ◽  
Blood Urea Nitrogen ◽  
Control Group ◽  
Urea Nitrogen ◽  
White Rats ◽  
Group I ◽  
Male Wistar Rats ◽  
Treatment Administration ◽  
The Mean

Incidence of drug overdose during treatment of acute disease consequently leads to serious renal damage. As supporting treatment, administration of herbal medicine and food dietary are frequently developed. This research aimed to prove how the administration of avocado juice could lower the BUN and creatinine level in white rats induced with toxic doses of meloxicam. Meloxicam is a non-steroidal anti-inflamatory drug (NSAID), which is effectively used as an anti-inflamatory, analgesic, and antipyretic. Twenty four (24) male Wistar rats were assigned to 4 groups of six rats each (n=6). 1 ml of CMC 1% was administered to Control Group I, 30 mg/kgBB meloxicam and 1 ml of CMC 1% to Control Grup II, 30 mg/kgBB meloxicam and avocado juice 5 g/kgBB/day to Treatment Group I, and 30 mg/kgBB meloxicam and avocado juice 10 g/kgBB/day to Treatment Group II. The study was conducted over 8 days, then the level of Blood Urea Nitrogen and creatinine of the white rats were examined on the 1st and 8th day. The results were analyzed by Anova Two Way With Replication, then followed by T-test (α = 0,05) if there were difference. The Anova Two Way With Replication test showed that the mean of the four groups either the levels of Blood Urea Nitrogen or creatinine was significantly decreased (p<0,05). The decrease of BUN in the treatment group I was 27,17 27,17mg/dl and 17,83mg/dl while the decrease of creatinine level was 0,983mg/dl and 0,713mg/dl. The conclusion of this study was that avocado juice decreases level of Blood Urea Nitrogen and creatinine in white rats which exposed toxic doses of meloxicam.

scholarly journals EFEKTIVITAS EKSTRAK ETANOL DAUN EFEKTIVITAS EKSTRAK ETANOL DAUN SIDAGURI (Sida rhombifolia L.) TERHADAP PENURUNAN KADAR ASAM URAT PADA TIKUS JANTAN GALUR WISTAR HIPERURISEMIA

2020 ◽  
Vol 3 (1) ◽  
pp. 29-37
Author(s):  
Bunga Rimta Barus
Keyword(s):  
Body Weight ◽  
Uric Acid ◽  
Ethanol Extract ◽  
Negative Control ◽  
Control Group ◽  
Weight Group ◽  
White Rats ◽  
Group I ◽  
Significant Difference ◽  
Sida Rhombifolia

Sidaguri leaves (Sida rhombifolia L.) Have been shown to contain flavonoid compounds, where they are efficacious as hyperuricemia. This study aims to determine effectiveness ethanol extract of sidaguri leaves as antihyperuricemia in white rats. The study was conducted using the method of potassium oxanate induction and measured using glucometers. 15 animals were divided into 5 groups, namely negative control, positive control, group I combination of ethanol extract of sidaguri leaves 50 mg / kg body weight, group II ethanol extract of sidaguri leaves 100 mg / kg body weight, group III ethanol extract of sidaguri leaves 200 mg / kg body weight for 5 hours. Examination of antihyperuricemia effects is done by comparing the uric acid levels value of the rats before and after treatment. The results of this study indicate that the three dose ethanol extract of sidaguri leaves provide an antihyperuricemia effect and based on one-way Anova, Tuckey and LSD data analysis, shows that the dose of 50 mg / kg body weight is effective as antihyperuricemia and does not have a significant difference (p> 0.05). The conclusion of this study is that the dose is the most effective in decreases uric acid levels in white rats, which is a dose of 200 mg / kg body weight because it mostly decreased uric acid levels.

scholarly journals The Effect of Black Garlic (Allium sativum Linn) on Cardiac and Aortic Histopathology in Experimental Studies in Obesity Rats

2021 ◽  
Vol 7 (2) ◽  
pp. 62-68
Author(s):  
Andreas Arie Setiawan ◽  
Fairuz Azmila Purnomo ◽  
Vega Karlowee ◽  
Noor Wijayahadi
Keyword(s):  
Body Weight ◽  
Wall Thickness ◽  
Experimental Studies ◽  
Optimal Dose ◽  
The Body ◽  
Heart Weight ◽  
Control Group ◽  
Sprague Dawley ◽  
Control Group Design ◽  
White Rats

ABSTRACTBackground: Obesity is a disorder or disease characterized by the accumulation of excess fat in the body due to an imbalance in energy intake that is used for a long time. Accumulation of fat can reduce adiponectin, causing cardiac hypertrophy, endothelial vasodilation, and other cardiovascular diseases. Black garlic have high antioxidants in the form of S-Allylcysteine(SAC) which functions to increase adiponectin. Objective: To determine the effect of Black garlic on the histopathological picture of the heart and aorta of obese rats. Methods: This study was an experimental study with a randomized post-test only design with control group design with 5 groups of male white rats Sprague Dawley (Rattus novergicus) fed High Fat Fructose. Diet (HFFD) enriched with 1.25% cholesterol and 0.5% cholic acid for 8 weeks and was given black garlic intervention at doses of 450 mg / 200BW, 900mg / 200BW and 1350mg200 / BW for 4 weeks. Results: Giving black garlic significantly reduced body weight of rats (p = 0.001), and the results did not significantly reduce heart weight (p = 0.147), aortic weight (p = 0.061), histopathological changes in heart wall thickness (p = 0.423) and aortic wall thickness (p = 0.802). The effective doses of black garlic in this study were 450 mg / 200 grams BW, 900 mg / 200 grams rat BW and 1350 mg / 200 grams BW of rats. The optimal dose is 900 mg / 200 grams BW. Conclusion: Black garlic gave a significant reduction in body weight of rats and no significant reduction in heart weight, aortic weight, cardiac and aortic histopathological features. 

scholarly journals Effectiveness of the use of sanifying set «SanStim» for disinfection eggs before incubation

2018 ◽  
Vol 20 (83) ◽  
pp. 226-229
Author(s):  
T.I. Fotina ◽  
H.A. Fotina ◽  
I. V. Kovalenko
Keyword(s):  
Growth And Development ◽  
Bacterial Infections ◽  
Guanidine Hydrochloride ◽  
Experimental Studies ◽  
Natural Resistance ◽  
Control Group ◽  
Bactericidal Action ◽  
Source Of Infection ◽  
Wide Range ◽  
Corrosive Activity

The most vulnerable place in poultry farms is incubation, microorganisms are able to survive the entire period of incubation and penetrate through the eggshell, to be the source of infection with embryos, to affect the reduction of the percentage of conditional young birds, growth and development in the early days of cultivation. The accumulated pathogenic microflora negatively affects the state of the immune system of the bird. Contaminated with conditionally pathogenic and pathogenic microflora egg is the main link in the transmission of all bacterial infections. In Ukraine, for the pre-incubation treatment of eggs, sodium hydroxide, formalin, chloramine, persicum C. are used. These agents exhibit bactericidal properties, but are toxic, have corrosive activity and are carcinogens. The «SunTime» kit consists of polyhexamethylene guanidine hydrochloride and a mixture of organic acid salts (lemon, amber, and apple). These substances provide an effective bactericidal action against a wide range of bacteria, are safe for poultry embryos and personnel, have a stimulating effect on the growth and development of the embryo, provide a prolonged bactericidal action. The purpose of the study of the effectiveness of a complex ecologically safe sanitizers set «SunSim», its application for pre-incubation processing of chicken eggs. In experimental studies, two groups were formed, each with n = 250. In order to disinfect the surface of the shell of the incubation egg, two times aerosol treatment of the experimental group using 0.3% of SunStim was carried out, the first time the sanitation of the incubation eggs was carried out before the laying on the incubation cabinet, the exposure was 40 minutes, the second final treatment was carried out on the 18th day of incubation, to extract, a working solution of organic acids (amber, lemon and apple) to stimulate embryonic viability and increase the natural resistance of the embryos. For the control group, formalin was used. After two times of the sanation of the incubation eggs with the SunStim set, the percentage of decontamination of the shell surface of the incubation eggs was 99.1 ± 0.5%. While analyzing the results of the incubation waste category of «dead and drowning» by 3,6% less compared with the control, and the category of «crippled» was not observed. The use of the SunStim set improves the quality of surface treatment of the shell of incubation eggs, increases the incubation eggs output by 4.5-5.0%, the average body weight of the received young animals in experimental groups exceeds control by 4%.

scholarly journals UJI TOKSISITAS SUB AKUT INFUS DAUN JARAK PAGAR (Jatropha curcas Linn.) TERHADAP GAMBARAN HISTOLOGI HATI MENCIT

2020 ◽  
Vol 4 (2) ◽  
Author(s):  
Surya Amal
Keyword(s):  
Mus Musculus ◽  
Jatropha Curcas ◽  
Liver Tissue ◽  
Block Design ◽  
Control Group ◽  
Cell Nuclei ◽  
Group Iii ◽  
Normal Limits ◽  
Group I ◽  
Membrane Cell

Penggunaan daun jarak pagar, khususnya sebagai obat antihipertensi, masih perlu didukung data toksisitas untuk menjamin keamanan pemanfaatannya. Telah dilakukan penelitian pengaruh infus daun jarak pagar (Jatropha curcas Linn.) terhadap gambaran histologi hati mencit (Mus musculus) yang diamati secara mikroskopik. Penelitian ini menggunakan 60 ekor mencit jantan yang dibagi dalam tiga kelompok konsentrasi : kelompok I, 5 % b/v; kelompok II, 10 % b/v; kelompok III, 20 % b/v ditambah satu kelompok kontrol. Kelompok I, II dan III serta kelompok kontrol masing-masing dibagi atas tiga subkelompok berdasarkan lama pemberian (15 hari, 30 hari, 45 hari). Masing-masing subkelompok terdiri dari 5 ekor mencit. Hasil pemeriksaan mikroskopik menunjukkan bahwa pemberian infus daun jarak pagar 5 % b/v, 10 % b/v dan 20 % b/v peroral sekali sehari dengan dosis 1 ml/30 gram berat badan memengaruhi struktur jaringan hati mencit. Pemberian infus daun jarak pagar 5 % b/v selama 15 hari menunjukkan struktur jaringan hati mencit yang masih dalam batas-batas normal. Kerusakan struktur jaringan hati mencit mulai terlihat setelah pemberian infus daun jarak pagar 5 % b/v selama 30 hari dan 45 hari yang meningkat dengan semakin besarnya konsentrasi dan lamanya masa pemberian.  Perhitungan secara statistik dengan menggunakan desain blok lengkap acak menunjukkan bahwa parameter susunan radier sel, sinusoid, membran sel, sitoplasma sel dan inti sel jaringan hati mencit mengalami kerusakan akibat pemberian infus daun jarak pagar 5 % b/v, 10 % b/v dan 20 % b/v dengan efek yang sangat berbeda nyata.   Kata kunci : jarak pagar, histologi hati, infus The use of Jatropha leaves, especially as an antihypertensive drug, still needs to be supported by toxicity data to ensure the safety of its use. Research on the effect of jatropha (Jatropha curcas Linn.) Infusion on mice (Mus musculus) histology was observed microscopically. This study used 60 male mice divided into three concentration groups: group I, 5% w / v; group II, 10% w / v; group III, 20% w / v plus one control group. Groups I, II and III and the control group each divided into three subgroups based on the length of administration (15 days, 30 days, 45 days). Each subgroup consists of 5 mice. Microscopic examination results showed that the administration of Jatropha leaf infusion of 5% w / v, 10% w / v and 20% w / v orally once a day at a dose of 1 ml / 30 grams of body weight affected the structure of the liver tissue of mice. The administration of Jatropha leaf infusion of 5% w / v for 15 days showed that the structure of the liver tissue of mice was still within normal limits. Damage to the structure of the liver tissue of mice began to be seen after administration of Jatropha leaf infusion of 5% w / v for 30 days and 45 days which increased with increasing concentration and length of administration. Statistical calculations using a randomized complete block design showed that the parameters of the cell radier, sinusoid, cell membrane, cell cytoplasm and liver cell nuclei of the mice suffered damage due to jatropha leaf infusion of 5% w / v, 10% w / v and 20 % b / v with a very different effect.   Keywords: jatropha, liver histology, infusion

UJI POTENSI EKSTRAK DAUN BENALU BATU (BEGONIA SP) TERHADAP KADAR GLUKOSA DALAM DARAH DAN GAMBARAN HISTOPATOLOGI PANKREAS TIKUS PUTIH JANTAN (RATTUS NORVEGICUS)

2020 ◽  
Vol 6 (2) ◽  
pp. 286
Author(s):  
Joni Tandi ◽  
Dela Erni Paerunan ◽  
Nurifa Nurifa ◽  
Yunlis Silintowe Kenta ◽  
Sri Mulyani
Keyword(s):  
Rattus Norvegicus ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Group Iii ◽  
Glucose Levels ◽  
White Rats ◽  
Group I ◽  
Ethanol Extracts

This study aims to determine the potential for glucose levels in the blood of ethanol extracts of the begonias (Begonia Sp.), the effect of the extract on the pancreatic histopathology of white rats (Rattus norvegicus) which was induced by streptozotocin, and the effective dose begonias’ extract leaves on the histopathological descriptions of pancreatic male white rats. This study was an experimental laboratory where the research group consisted of group I (the control group) as a normal control was given a standard feed, group II (negative control) was given a 0.5% Na CMC suspension, group III (positive control) was orally given glibenclamide and experimental groups IV, V, and VI (doses 50, 100, and 200 mg/kg BW). Were given ethanol extract of begonias leaves. The results showed that the ethanol extract of the begonias (Begonia Sp.) contained secondary metabolite compounds, namely flavonoids, alkaloids, saponins, polyphenols, and tannins. Ethanol extract of begonias leaves at adose of 50 mg/kg BW was an affective dose in reducing blood glucoselevels by an avarage of 101,8 mg/dL) and a dose of 50 mg/kg BW can affect the histopathological picture of the pancreas in STZ induuced rats with an average damage value of 2 from a maximum of 4.

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