ABSTRAK Kolostrum atau susu jolong yang keluar pertama kali sering dibuang karena masih dianggap kotoran ASI, sehingga masih banyak ibu yang memberikan susu formula bagi bayinya padahal kolosotrum banyak mengandung gizi dan zat kekebalan bagi tubuh (Bahiyatun, 2009). Masalah penelitian ini adalah masih tingginya ibu yang tidak mengerti tentang kolostrum. Tujuan penelitian adalah mengetahui pengaruh Health Education (HE) tentang pemberian kolostrum pada 1 jam pertama terhadap peningkatan pengetahuan ibu hamil.Desain penelitian ini adalah Pra Eksperiment dengan pendekatan One Grup Pratest-Postest Design. Sampelnya sebanyak 27 orang dengan teknik simple random sampling. Variabel independent pemberian Health Education tentang kolostrum dan variabel dependent pengetahuan ibu hamil. Instrumen yang digunakan adalah SAP, leaflet dan lembar kuesioner. Analisa data menggunakan Wilcoxon Sign Rank Test dengan taraf signifikan 0,05.Hasil penelitian menunjukkan sebelum diberikan HE hampir seluruh (81,5%) ibu hamil memiliki pengetahuan kurang tentang pemberian kolostrum pada 1 jam pertama, dan setelah diberikan HE lebih dari sebagian (59,3%) berpengetahuan cukup. Hasil Wilcoxon Sign Rank Test didapatkan p= 0,001 dimana p0,05. Maka H1 diterima artinya ada pengaruh HE tentang pemberian kolostrum pada 1 jam pertama terhadap peningkatan pengetahuan ibu hamil.Melihat dari hasil penelitian, maka HE sangat penting diberikan untuk meningkatkan pengetahuan ibu hamil dalam pemberian kolostrum pada bayi terutama pada 1 jam pertama, sehingga bayi terjamin untuk mendapatkan ASI eksklusif serta mengurangi angka kesakitan maupun kematian pada bayi. Kata kunci : Health Education, Pengetahuan, Kolostrum ABSTRACT Colostrum or weaned that out the first time are often discarded because they are considered dirt breast milk, so there are many mothers to give formula to their babies even though colosotrum contain many nutrients and substances for the body's immune (Bahiyatun, 2009). The problem of this study is still high mother who does not understand about colostrum. The research objective was to determine the effect of Health Education (HE) on the provision of colostrum in the first hours of the first to the increased knowledge of pregnant women.The research design was Pre Experiment with One Group approach Pratest-Posttest Design. The sample as many as 27 people with simple random sampling technique. The independent variable is giving Health Education about colostrum and the dependent variable is knowledge of pregnant women. The instruments used were SAP, leaflet and questionnaire. Data were analyzed using the Wilcoxon Sign Rank Test with significance level of 0.05.Results showed before being given HE almost all (81.5%) of pregnant women have less knowledge about the provision of colostrum in the first hours of the first, and after being given HE more than most (59.3%) are knowledgeable enough. Wilcoxon Sign Rank Test results obtained p = 0.001 where p 0.05. Then there is H1 accepted meaning HE influence on the provision of colostrum in the first hours of the first to the increased knowledge of pregnant women.Judging from the results of the study, then HE is essential given to improve the knowledge of pregnant women in giving colostrum to the baby, especially in the first 1 hour, so that the baby is guaranteed to get exclusive breastfeeding and reducing morbidity and mortality in infants. Keywords: Health Education, Knowledge, Colostrum