scholarly journals Increased Estradiol Levels Effect on Leptin Expression in Midluteal Endometrial Tissue of Macaca Nemestrina (Southern Pig-Tailed Macaques)

2021 ◽  
Author(s):  
Nurhuda Sahar ◽  
Adriana Viola Miranda ◽  
Afif Rasyad ◽  
Karina Rahmaningrum ◽  
Kusmardi Kusmardi ◽  
...  
Keyword(s):  
Previous History ◽  
Intervention Group ◽  
Controlled Ovarian Hyperstimulation ◽  
Endometrial Tissue ◽  
Reproductive Age ◽  
Macaca Nemestrina ◽  
Control Group ◽  
Serum Estradiol ◽  
Imagej Software ◽  
Leptin Expression

Objective: To measure the effect of serum estradiol (E2) levels on leptin expression in Macaca nemestrina (southern pig-tailed macaque) endometrial tissue. Materials and methods: This study used paraffin-embedded midluteal phase endometrial tissue blocks of Macaca nemestrina from previous study. Included subjects were 15 female macaques of reproductive age (8-10 years) with a previous history of producing offspring, which were divided into four groups: groups administered with 30 IU, 50 IU, and 70 IU r-FSH (intervention group), and no r-FSH (control group). The stimulation was done following GnRH agonist long protocol. Staining was done using immunohistochemistry. Leptin expression was measured using immunohistochemistry (IHC) Profiler plugin of ImageJ software and counted semi-quantitatively as Histological Score (Hscore). Results: Correlation between E2 concentration to stromal leptin expression was observed (p=0.043). Conclusion: Serum estradiol concentration is found to be correlated with leptin expression in Macaca nemestrina, suggesting a mechanism of decreasing endometrial receptivity among women undergoing controlled ovarian hyperstimulation.

189 THE EFFECT OF VITAMIN D SUPPLEMENTATION ON ANTI-MULLERIAN HORMONE LEVELS IN REPRODUCTIVE-AGE WOMEN

2015 ◽  
Vol 27 (1) ◽  
pp. 185 ◽  
Author(s):  
M. Taheri ◽  
M. Modarres ◽  
A. Abdollahi
Keyword(s):  
Vitamin D ◽  
Vitamin D Deficiency ◽  
Intervention Group ◽  
Serum Levels ◽  
Vitamin D Supplementation ◽  
T Test ◽  
Reproductive Age ◽  
Control Group ◽  
Post Intervention ◽  
Reproductive Age Women

Vitamin D deficiency has been correlated with the infertility and lower clinical pregnancy following IVF. Anti-Mullerian hormone (AMH) plays a key role during follicle development; it has been recognised as a predictor of regular ovulation and probably IVF success. Considering the critical need for experimental human study to investigate the impact of vitamin D supplementation on ovulatory function, the aim of this study was to demonstrate the effectiveness of the vitamin D supplementation on AMH serum levels among reproductive-age women with vitamin D deficiency. 195 reproductive women (18–35 year-old) with confirmed vitamin D deficiency [serum 25(OH)D <75 nmol L–1] and without diagnosed polycystic ovary syndrome (PCOS) were enrolled to this controlled clinical trial. Participants were randomly assigned to a control group (n = 96) or an intervention group (n = 99). Women in the intervention group used 2000 IU day–1 vitamin D drops for 15 weeks. 19 participants were missed during the follow-up; finally the numbers of women in the intervention and control groups were 91 and 85, respectively. At the beginning of the study and after the intervention, 25-hydroxyvitamin D and AMH serum levels were quantified using enzyme immunoassay (EIA; Immunodiagnostic Systems, Boldon, UK) and ELISA (Beckman-Coulter Inc., Fullerton, CA, USA) methods respectively. The post-intervention AMH measurement was performed after 2–5 weeks in the same day-of-cycle on which basal AMH measurement was done. Paired t-test, independent t-test, and Pearson correlation were used as appropriate and a P-value of less than 0.05 was considered significant. Significantly low AMH levels were seen in the vitamin D deficient women of this study (14.46 ± 11.92 pmol L–1 in control group and 14.09 ± 11.52 pmol L–1 in intervention group). After the intake of vitamin D supplementation in intervention group, AMH levels were increased to 24.89 ± 12.47 pmol L–1, which were significantly different from the 15.43 ± 13.03 pmol L–1 in control group (P < 0.001). Correlation coefficients for AMH with pre-intervention and post-intervention vitamin D were r = 0.489 and r = 0.599 respectively (P < 0.001). Treatment of vitamin D deficiency increases AMH to the optimum levels. Vitamin D deficient women had low levels of AMH. These findings support other studies which found a correlation of poor IVF outcomes with low vitamin D levels. Vitamin D supplementation could be useful in the improvement of controlled ovarian hyper-stimulation/IVF outcomes in case of vitamin D deficiency.

scholarly journals Efektivitas langkah-langkah peregangan (stretching) untuk menurunkan nyeri dismenore di wilayah Sleman Yogyakarta

2020 ◽  
Vol 9 (1) ◽  
pp. 1
Author(s):  
Sherkia Ichtiarsi Prakasiwi
Keyword(s):  
Intervention Group ◽  
Sampling Technique ◽  
Reproductive Age ◽  
Bivariate Analysis ◽  
Control Group ◽  
P Value ◽  
Cross Sectional ◽  
Test Study ◽  
Spss Software ◽  
The Brain

Dysmenorrhea is an imbalance of the hormone progesterone in the blood, causing pain to arise. Women have experienced 90% of dysmenorrhea, this disturbs 50% of women of reproductive age and 60-85% in adolescence, which results in many absences in schools and offices. Non-pharmacological solutions are seen as safer, one of which is stretching which aims to make muscles and joints flexible and elastic. This is due to increased levels of endorphins, epinephrine, dopamine and serotonin produced by the brain due to exercise. This study aims to determine stretching steps to reduce dysmenorrhea pain in the Sleman region. The method of this research is a different test study with a cross sectional type of research. The research sample was 60 female students. The sampling technique was purposive sampling, bivariate analysis techniques using the Mann Whitney statistical test using SPSS software. The results of the Mann Whitney test in the control group and the intervention group showed a p-value of 0,000 (ɑ <0.05) indicating that there were differences between the control and intervention groups in decreasing the level of pain in dysmenorrhea where the mean pain level of dysmenorrhea was 4 50 while in the intervention group the average is only 0.13 so that it gets ∆ mean of -4.37. This proves that stretching is more influential in decreasing the level of pain in dysmenorrhea than in stretching.

scholarly journals The Effects of Classical Music based Chakra Meditation on the Symptoms of Premenstrual Syndrome

2015 ◽  
Vol 2 (3) ◽  
Author(s):  
Poornima Viswanathan ◽  
Nishal Pinto ◽  
Poornima Viswanathan
Keyword(s):  
Menstrual Cycle ◽  
Premenstrual Syndrome ◽  
Classical Music ◽  
Intervention Group ◽  
Symptom Clusters ◽  
Reproductive Age ◽  
Control Group ◽  
Post Test ◽  
And Control ◽  
Experimental Group

Premenstrual syndrome (PMS) is a very common condition, occurring in women of reproductive age. This is an important source of stress for women, significantly affecting their regular functioning. Previous studies have shown that alternative forms of therapy like meditation have an effect on the symptoms associated with PMS. However, the direct link between the two has not been focused on by the larger group of researchers. In this study, the researcher intended to study the effects of classical music based chakra meditation on the symptoms of premenstrual syndrome. The sample of the study comprised of 40 college students who were screened for premenstrual syndrome based on the Shortened Premenstrual Assessment Form (SPAF) and Calendar of Premenstrual Experiences(COPE) which they were expected to fill in for one menstrual cycle. The participants who qualified for the study were allotted to the intervention group and control group. The intervention group was expected to practice a 15 minute music based chakra meditation, everyday, for one menstrual cycle, during which they also filled in the COPE. The control group was expected to fill the COPE for one menstrual cycle. Both the groups filled in the SPAF after the completion of the menstrual cycle. The results on the SPAF and COPE showed that there was significant reduction in all the post-test symptom clusters of the experimental group, compared with the pre-test symptoms. Thus, this study demonstrates that music based chakra meditation can have significant healing effects on the symptoms of premenstrual syndrome.

scholarly journals Lifestyle Intervention for Overweight/Obese Pregnant Women with Polycystic Ovarian Syndrome: Lessons and Challenges

Obesity Facts ◽  
2021 ◽  
Vol 14 (4) ◽  
pp. 405-414
Author(s):  
Cheng Liu ◽  
Lirui Zhang ◽  
Wei Zheng ◽  
Xin Liang ◽  
Li Zhang ◽  
...  
Keyword(s):  
Weight Gain ◽  
Pregnant Women ◽  
Lifestyle Intervention ◽  
Intervention Group ◽  
Perinatal Outcomes ◽  
Overweight And Obesity ◽  
Reproductive Age ◽  
Control Group ◽  
Good Compliance ◽  
Diet And Exercise

<b><i>Introduction and Objective:</i></b> Polycystic ovary syndrome (PCOS) is the most common reproductive disorder in women of reproductive age, and overweight and obesity are highly prevalent in women with PCOS. This study aims to explore whether lifestyle intervention can improve gestational weight gain (GWG), glucolipid metabolism, and perinatal outcomes in overweight/obese pregnant women with PCOS. <b><i>Methods:</i></b> This study is a randomized controlled trial that included overweight and obese pregnant women with PCOS who met the inclusion criteria of 8–12 gestational weeks. They were randomly allocated to the intervention group and the control group. Women in the intervention group were given individualized counseling on diet and exercise from a trained dietitian and followed up regularly by a trained dietitian. Women in the control group received guidance on diet and exercise in the form of group education. <b><i>Results:</i></b> A total of 296 pregnant women were enrolled in the study, including 164 in the intervention group and 132 in the control group. GWG was 11.93 ± 5.67 kg in the intervention group and 11.86 ± 5.35 kg in the control group and did not differ between the 2 groups. According to the per-protocol analyses, women with good compliance had a lower weight gain (10.11 ± 5.56 vs. 12.70 ± 5.31, <i>p</i> = 0.0042). The incidence of gestational diabetes mellitus and other perinatal outcomes did not differ between the 2 groups. For the lipid profile, we did not find significant improvement in the intervention group. <b><i>Conclusions:</i></b> Our study showed that lifestyle intervention of diet and exercise did not affect GWG, glucolipid metabolism, and perinatal outcomes of overweight/obese pregnant women with PCOS. However, women with good compliance can benefit from the lifestyle intervention for GWG. We believe that future studies should focus on trial design and increasing compliance to improve the quality of the study.

scholarly journals Thyroid Dysfunction and Autoantibodies Association with Hypertensive Disorders during Pregnancy

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Azin Alavi ◽  
Khadijeh Adabi ◽  
Sepideh Nekuie ◽  
Elham Kazemi Jahromi ◽  
Mehrdad Solati ◽  
...  
Keyword(s):  
Thyroid Function ◽  
Thyroid Dysfunction ◽  
Gestational Hypertension ◽  
Previous History ◽  
Reproductive Age ◽  
Control Group ◽  
Thyroid Function Tests ◽  
Hypertensive Patients ◽  
Hypertensive Disorders ◽  
Low T3

Background. Thyroid dysfunction and autoimmunity are relatively common in reproductive age and have been associated with adverse health outcomes for both mother and child, including hypertensive disorders during pregnancy.Objective. To survey the relation between thyroid dysfunction and autoimmunity and incidence and severity of pregnancy-induced hypertensive disorders.Method. In this case control study 48 hypertensive patients in 4 subgroups (gestational hypertension, mild preeclampsia, severe preeclampsia, eclampsia) and 50 normotensive ones were studied. The samples were nulliparous and matched based on age and gestational age and none of them had previous history of hypertensive or thyroid disorders and other underlying systemic diseases or took medication that might affect thyroid function. Their venous blood samples were collected using electrochemiluminescence and ELISA method and thyroid hormones and TSH and autoantibodies were measured.Results. Hypertensive patients had significant lower T3 concentration compared with normotensive ones with mean T3 values152.5±48.93 ng/dL,175.36±58.07 ng/dL respectively. Anti-TPO concentration is higher in control group6.07±9.02 IU/mL compared with2.27±2.94 IU/mL in cases.Conclusion. The severity of preeclampsia and eclampsia was not associated with thyroid function tests. The only significant value was low T3 level among pregnancy, induced hypertensive patients.

scholarly journals Endometrial Cytokines in Women with Reproductive Disorders

2021 ◽  
Vol 11 (4) ◽  
pp. 526-531
Author(s):  
Irina Danusevich ◽  
Lyudmila Lazareva ◽  
Uliana Nemchenko ◽  
Lyubov Kolesnikova
Keyword(s):  
Bacterial Flora ◽  
Fold Increase ◽  
Endometrial Tissue ◽  
Reproductive Age ◽  
Control Group ◽  
Reproductive Disorders ◽  
Medical Apparatus ◽  
Tnf Α ◽  
Group 2 ◽  
Group 1

The purpose of this research was to study changes in endometrial cytokine concentrations in women suffering from reproductive disorders with and without chronic endometritis (CE) to justify pathogenetic treatment. Methods and Results: The study included 100 women of reproductive age with reproductive disorders. Group 1 included 50 patients with reproductive disorders and CE; Group 2 included 50 patients with reproductive disorders and without CE. Later on, all patients were divided into the following subgroups: Sub1A (n=31), and Sub2A (n=16) with an isolated bacterial flora, Sub1B (n=19) and Sub2B (n=34) with the absence of bacterial flora. The control group consisted of 31 fertile women. Endometrial aspiration pipe biopsy was performed on days 4-9 of the menstrual cycle (middle proliferative phase) using a disposable intrauterine probe (Taizhou Kechuang Medical Apparatus Co., Ltd, China) followed by histological examination of endometrial tissue. Laboratory diagnostics for sexually transmitted infections (STIs) was performed using the bacterial culture method. For the diagnosis of viral infection (HPV, HSV, CMV), cervical samples were studied using PCR. If STIs were detected, the patients were excluded from further research. Ultrasound examination of the pelvic organs was performed using the Aloka-5500 device with a 7MHz vaginal probe in two-dimensional visualization mode. The concentration of cytokines (IL-1β, INF-γ, TNF-α, ILs-4,6,8,10) in the endometrium was determined using the Protein Contour test systems (Saint Petersburg) and Multiskan EX ELISA Analyzer (Germany). In both groups, reproductive disorders were accompanied by hypoprogesteronemia and relative hyperestrogenemia, significantly apparent in CE. We found a 3-fold increase in the level of tissue pro- and anti-inflammatory cytokines (IL-1β, IL-4,6,10, INF-γ), and a 4-fold increase in the level of TNF-α and IL-8 in Group 1, compared to the CG. In Group 2, we found a 1.4-fold increase in the levels of IL-1β and INF-γ, compared to the CG. In Sub 1a, the levels of IL-6 and IL-8 were significantly higher than in the control group. In Sub1A, the isolated bacterial flora caused a cytokine inflammatory response characterized by a significant increase in the concentration of INF-γ and TNF-α, compared to Sub2A and Sub2B (P<0.05). In Sub1A, we found a tendency towards a decrease in the tissue levels of IL-4 compared to Sub1B and Sub2B; the IL-10 level was significantly lower than in Sub2B (P=0.0009) Conclusion: The results obtained in the present study showed the peculiarities of changes in cytokines at the level of endometrial tissue both in chronic inflammation of the endometrium and in its absence in women with reproductive disorders. The severity of the immune response is significantly higher in patients with CE, with the most significant change in the role of IL-10.

scholarly journals O-142 COVID19-free endometrium: Undetectable viral RNA in endometrial biopsies from positive symptomatic SARS-CoV-2 women

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
L De Miguel-Gómez ◽  
M Romeu ◽  
N Pellicer ◽  
A Faus ◽  
A Pellicer ◽  
...  
Keyword(s):  
Menstrual Cycle ◽  
Endometrial Tissue ◽  
Viral Rna ◽  
Reproductive Age ◽  
N Gene ◽  
Control Group ◽  
Rt Pcr ◽  
Short Term ◽  
Recent Emergence ◽  
Registration Number

Abstract Study question Does SARS-CoV-2 infect the endometrial tissue in women with coronavirus disease 2019 (COVID-19)? Summary answer Symptomatic women with COVID-19 report no presence, in the short term, of viral RNA from SARS-CoV-2 in the endometrium. What is known already The recent emergence of COVID-19, caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has not allowed yet to establish putative relations between this disorder and other existing pathologies. It is the case with fertility problems and the reproductive organs, including a possible endometrial disorder caused by the virus. Thus, an important step is to elucidate the viral presence in different human tissues to improve diagnostics, prevention and/or treatment. The hypothesis of the possible infection of the endometrial tissue comes from the described expression of ACE2 protein in the human endometrium, mainly in stromal cells during the secretory phase. Study design, size, duration 15 endometrial biopsies from symptomatic and hospitalized women with COVID-19 were collected. Endometrial samples were obtained from August to November 2020 at the Hospital Universitari i Politècnic La Fe (Valencia, Spain); the project received the approval of the hospital’s medical ethics committee (registration number: 2020-268-1). The main objective was to study by real-time PCR (RT-PCR) the presence of viral RNA from SARS-CoV-2 as well as the expression of ACE2 receptor on the endometrial tissue. Participants/materials, setting, methods 15 women in the reproductive age (24-46 years) accepted to participate in the study and signed the informed consent. All these patients tested positive for SARS-CoV-2 by RT-PCR of nasopharyngeal swabs (1-17 days before the biopsy collection) and were hospitalized due to health complications (pneumonia) derived from COVID-19. Endometrial biopsies were taken by aspiration and preserved in RNA-later until -80ºC cryopreservation in a biobank; RNA was extracted for RT-PCR for N1, N2, and ACE2 genes. Main results and the role of chance The 15 recruited patients represented the different phases of the menstrual cycle: proliferative (n = 3) and secretory (n = 10); 2 patients had amenorrhea. The viral RNA for SARS-CoV-2, measured by the detection of N1 and N2 gene targets (fragments of N gene, from the viral nucleocapsid) by RT-PCR methodology, was undetectable in all the endometrial biopsies analyzed (n = 15). In all the cases the housekeeping gene RPP30 was used as positive control and to check RNA integrity. To correlate the presence or absence of SARS-CoV-2 with the organ-specific expression of ACE2 (angiotensin-converting enzyme 2), the main postulated entry receptor of SARS-CoV-2, the endometrial RNA was also analyzed by RT-PCR for the ACE2 receptor gene. This gene was only detectable in 10 of the 15 biopsies, and the levels ranged from 28.65 to 36.19 Ct values, revealing a very low expression of ACE2 in the tissue. Moreover, ACE2 results did not report any correlation with the phase of the menstrual cycle. Limitations, reasons for caution These results imply endometrium is safe from SARS-CoV-2 infection, at least in the short term. All the endometrial samples were taken at maximum of 17 days after a positive test by RT-PCR of nasopharyngeal swabs (to note that all were hospitalized during the early stages of the disease). Wider implications of the findings In conclusion, the SARS-CoV-2 RNA is not present in the human endometrial tissue of positive patients. This hypothesis was reinforced by the low ACE2 receptor levels. However, an in-depth genetic analysis comparing to a negative control group could elucidate a systemic affectation of the endometrium, despite the negative RT-PCR results. Trial registration number not applicable

scholarly journals ASSESSMENT OF THE LEVEL OF ALPHA-2 MICROGLOBULIN OF FERTILITY AT THE SYSTEMIC AND TISSUE LEVELS AS AN INDICATOR OF THE FUNCTIONAL STATE OF THE ENDOMETRIUM

2018 ◽  
pp. 26-30
Author(s):  
Yu. A. Lyzikova ◽  
N. M. Golubykh ◽  
A. E. Kozlov
Keyword(s):  
Normal Values ◽  
Endometrial Tissue ◽  
Reproductive Age ◽  
Control Group ◽  
Study Group ◽  
Reproductive Dysfunction ◽  
Female Patients ◽  
Estradiol Concentration ◽  
Concentration Levels

Objective: to determine the level of alpha-2 microglobulin of fertility in the serum and endometrium of female patients of reproductive age. Material and methods . 30 female patients of reproductive age were included in the study. According to the results of clinical and case history data, 20 (66.67 %) patients with reproductive dysfunction were included in the study group, 10 (33.33 %) healthy patients were included in the control group. The concentration levels of hormones (FSH, LH, testosterone, prolactin, estradiol, progesterone, fertility alpha-2 microglobulin) in the blood serum and the level of alpha-2 microglobulin of fertility in the endometrial tissue were determined by the method of enzyme immunoassay. Results . The female patients with reproductive dysfunction revealed changes in the concentration of sex hormones. The estradiol concentration was 0.22 (0.21-0.24) nmol/l in the study group of the patients, and 0.25 (0.24-0.28) nmol/l in the healthy patients (z = -3.37, p = 0.0007). The progesterone concentration was 9.57 (7.60-53.54) nmol/l in the study group of the patients and 5.37 (3.59-26.44) nmol/l in the healthy women (z = 1.78, p = 0.07). The level of alpha-2 microglobulin of fertility did not differ significantly in the patients of both the groups. The level of alpha-2 microglobulin of fertility in the endometrium was 0.87 (0.69-1.38) ng/ml in the female patients of the study group and 1.82 (1.38-5.81) ng/ml in the patients of the control group (z = -2.31, p = 0.02). Conclusion . The obtained results are indicative of the involvement of the endometrium in the development of reproductive dysfunction. The data on the levels of alpha-2 microglobulin of fertility in the endometrial tissue are promising for further study and determination of the boundaries of its normal values. Further research in this area will facilitate the development of a test to predict successful implantation.

scholarly journals Expression of E-Cadherin in Pig-Tailed Monkey (Macaca nemestrina) Endometrium after Controlled Ovarian Hyperstimulation

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Nurhuda Sahar ◽  
R. Muharam ◽  
Andhea Debby Pradhita ◽  
Rosalina Thuffi ◽  
Wa Ode Zulhulaifah ◽  
...  
Keyword(s):  
Success Rate ◽  
Steroid Hormones ◽  
Implantation Rate ◽  
Controlled Ovarian Hyperstimulation ◽  
Macaca Nemestrina ◽  
Control Group ◽  
Ovarian Hyperstimulation ◽  
Cycle Group ◽  
Rate Research ◽  
E Cadherin

An increase of steroid hormones in controlled ovarian hyperstimulation (COH) procedures is reducing the success rate in assisted reproductive technology (ART), and this includes the pregnancy rate and/or implantation rate. Research has found that the decrease in the success rate occurred due to the decreased expression of the protein that is needed to prepare the endometrium so that the embryo could attach. The aim of the study was to analyse the changes in E-chaderin expression due to COH and its relations with increased level of steroid hormones as one of the proteins in the endometrium. There were 13 samples of stored biological tissue from Macaca nemestrina endometrial tissue; came from one group of natural cycles as the control group ( n = 4 ) and three groups of stimulated cycles. The first stimulated cycle group was injected by a 30 IU dose of rFSH ( n = 2 ). The second stimulated cycle group was injected by a 50 IU dose of rFSH ( n = 4 ). The third stimulated cycle group was injected by a 70 IU dose of rFSH ( n = 3 ). The expression of E-cadherin was measured by the immunohistochemistry (IHC) technique. Estradiol (E2) and progesterone (P4) levels were assessed using ELISA and have already been done. The IHC staining expression of E-cadherin was found in the cytoplasm of glandular epithelium. Immunostaining measurement used the H_SCORE. We found that the expression of E-cadherin within the group was not significantly different ( p value: 0.178). Similarly, both the correlation between the estradiol level with E-cadherin and the correlation between the progesterone level with E-cadherin were not significantly different ( p value: 0.872 and p value: 0.836). The conclusion is that the level of E-Cadherin expression in the endometrium that were taken in themiddle secretion phase not affected by the dose regimen that given. In addition, the level of expression is not influenced by the increase of serum E2 and P4 levels.

scholarly journals The Effect of Youth's Healthy Packages on Premenstrual Syndrome

2021 ◽  
Vol 3 (2) ◽  
pp. 35-41
Author(s):  
Sri Mulyati ◽  
Ida Widiawati
Keyword(s):  
Premenstrual Syndrome ◽  
Intervention Group ◽  
Simple Random Sampling ◽  
Physical Symptoms ◽  
Reproductive Age ◽  
Control Group ◽  
Women Of Reproductive Age ◽  
Chi Square ◽  
Healthy Adolescent ◽  
Quasi Experimental

Abstract— Most women of reproductive age have premenstrual symptoms which consist of emotional and physical symptoms. Many factors can affect PMS including physical activity, nutrition such as drinks containing calcium and ginger, massage and other factors. This study aims to determine the effect of a healthy package consisting of (exercise, giving ginger milk and effleurage massage) on premenstrual syndrome. The design of this study was a quasi-experimental pre-post with control group. The research variables were measured in 2 groups, There are the intervention group and the control group.  The number of samples in this study  consisted of 38 girls in the intervention group and 38 girls in the control group with  simple random sampling. The analysis used  univariate (frequency distribution) and bivariate (T Test, Chi-Square). The results showed that there was an influence between the effect of a healthy adolescent package (consisting of exercise, giving ginger milk and eflleurage massage) on premenstrual syndrome. Keywords— Pre Menstrual Syndrome, Exercise, Ginger Milk, Eflleurage Massage  

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