scholarly journals PRODUCTION OF ANTIBODY IgY ANTI-c-Myc IN CHICKEN EGGS

2015 ◽  
Vol 2 (1) ◽  
pp. 330
Author(s):  
Salomo Hutahaean
Keyword(s):  
Bioinformatics Analysis ◽  
Epitope Prediction ◽  
Keyhole Limpet Hemocyanin ◽  
Chicken Breast ◽  
Curve Pattern ◽  
The Third ◽  
Sigmoid Curve ◽  
Incomplete Freund’S Adjuvant ◽  
Immunization Booster ◽  
Chicken Eggs

<p>Bioinformatics analysis has been performed on c-Myc peptide sequences to predict its epitopes in order to design antigen for IgY anti-c-Myc production in chickens. Peptide sequences for human c-Myc protein obtained from the NCBI database were analyzed with ProPred-1 softwares. Manual screening has been conducted on the prediction results.One candidate of c-Myc epitope, EQKLISEEDL, have been selected and prepared as an antigen, by adding one molecule of Cystein to the peptide, and conjugated it with Keyhole limpet hemocyanin (KLH). Twelve hens, aged 22-24 weeks, were immunized a week after their first eggs were produced. Antigen diluted in aquabidest and emulsified with Freund’s Complete Adjuvant. The suspension was injected into the area of the chicken breast, subcutaneously or intramuscularly, with a dose of 0.1 mg for the first immunization. Booster injection performed on days 10, 20, and 30 with the same volume of emulsion, but using Incomplete Freund’s Adjuvant and the amount of antigen half of the first immunization. Extraction of IgY from egg has been carried out using kits from Gallus Immunotech. IgY levels quantified using a spectrophotometer, absorbance measured at 280 nm. The results showed that chicken antibody production gave rise to a sigmoid curve pattern, just like in mammals. The IgY content of the egg was stable at high levels (&gt; 5 mg / ml yolk) after the third immunization. In the intramuscular group, the immune response seems to work earlier than in the subcutaneous group, but eventually the subcutaneous group reaches the same level, and even higher. In general, from an egg produced after the second booster it can be extracted at least 50 mg of IgY. Higher yields could be obtained when IgY was extracted from egg 2 to 3 days after the third booster. </p><p>Keywords: epitope prediction, c-Myc, IgY antibody, ProPred-1</p>

Prediction of the Omp16 Epitopes for the Development of an Epitope-based Vaccine Against Brucellosis

2019 ◽  
Vol 19 (1) ◽  
pp. 36-45 ◽  
Author(s):  
Marzieh Rezaei ◽  
Mohammad Rabbani-khorasgani ◽  
Sayyed Hamid Zarkesh-Esfahani ◽  
Rahman Emamzadeh ◽  
Hamid Abtahi
Keyword(s):  
Immune Responses ◽  
B Cell ◽  
Dairy Products ◽  
Bioinformatics Analysis ◽  
Epitope Prediction ◽  
Animal Husbandry ◽  
Zoonotic Diseases ◽  
Recombinant Vaccines ◽  
Protective Capacity ◽  
Tertiary Structures

Background:Brucellosis is an infectious disease caused by Brucella bacteria that cause disease in animals and humans. Brucellosis is one of the most common zoonotic diseases transmitted from animals-to-human through direct contact with infected animals and also consumption of unpasteurized dairy products. Due to the wide incidence of brucellosis in Iran and economical costs in industrial animal husbandry, Vaccination is the best way to prevent this disease. All of the available commercial vaccines against brucellosis are derived from live attenuated strains of Brucella but because of the disadvantage of live attenuated vaccines, protective subunit vaccine against Brucella may be a good candidate for the production of new recombinant vaccines based on Brucella Outer Membrane Protein (OMP) antigens. In the present study, comprehensive bioinformatics analysis has been conducted on prediction software to predict T and B cell epitopes, the secondary and tertiary structures and antigenicity of Omp16 antigen and the validation of used software confirmed by experimental results.Conclusion:The final epitope prediction results have proposed that the three epitopes were predicted for the Omp16 protein with antigenicity ability. We hypothesized that these epitopes likely have the protective capacity to stimulate both the B-cell and T-cell mediated immune responses and so may be effective as an immunogenic candidate for the development of an epitope-based vaccine against brucellosis.

Effect of sweet almond suspension as anti-inflammatory in experimental infected mice with arthritis

2012 ◽  
Vol 36 (2) ◽  
pp. 98-105
Author(s):  
Lubna A. Kafi
Keyword(s):  
Knee Joint ◽  
Oral Treatment ◽  
Positive Control ◽  
Negative Control ◽  
Histopathological Study ◽  
Histopathological Changes ◽  
The Third ◽  
Incomplete Freund’S Adjuvant ◽  
Test Level ◽  
Sweet Almond

The current study was conduct to determine the effects of oral treatment of sweet Almond Suspension (SAS) on induced arthritis by Incomplete Freund’s Adjuvant (IFA).Seventy mice, with close age and weight were used; they were equally divided in to 7 groups (10 mice per group). The first group served as negative control (non infected – non treated (NINTC). The second group was the positive control (infected non treated, (NINTC) the third and fourth groups were those treated with 1.42 or 2.84 g/kg of SAS respectively. The fifth group was treated with voltarin (ITV), while the sixth and seventh groups were treated with the same closes of SAS but before infection (Prophylactic infected groups, PI1, PI2).The size of knee joint, carrageenan test, level of alkaline phosphatase and histopathological changes in the knee joint used as parameters to compare between groups. The results showed that SAS was able to subside signs of arthritis by decreasing the size of knee and decrease the formation of edema which was induced by injection of carrageenan in the paw of the animal, Histopathological study showed that joints of treated groups by SAS had no signs of arthritis. However, there was slight infiltration of netrophile in treatment and prophylactic group.

scholarly journals Bioinformatics Analysis Identifies a Small ORF in the Genome of Fish Nidoviruses of Genus Oncotshavirus Predicted to Encode a Novel Integral Protein

2021 ◽  
Vol 12 (4) ◽  
pp. 753-764
Author(s):  
Frederick Kibenge ◽  
Ashley McKibbon ◽  
Molly Kibenge ◽  
Yingwei Wang
Keyword(s):  
Bioinformatics Analysis ◽  
Signal Sequence ◽  
Open Reading Frame ◽  
Type I ◽  
Structural Protein ◽  
Genome Sequence Analysis ◽  
Reading Frame ◽  
The Third ◽  
E Protein ◽  
Small Open Reading Frame

Genome sequence analysis of Atlantic salmon bafinivirus (ASBV) revealed a small open reading frame (ORF) predicted to encode a Type I membrane protein with an N-terminal cleaved signal sequence (110 aa), likely an envelope (E) protein. Bioinformatic analyses showed that the predicted protein is strikingly similar to the coronavirus E protein in structure. This is the first report to identify a putative E protein ORF in the genome of members of the Oncotshavirus genus (subfamily Piscavirinae, family Tobaniviridae, order Nidovirales) and, if expressed would be the third family (after Coronaviridae and Arteriviridae) within the order to have the E protein as a major structural protein.

scholarly journals Comparison of Polyclonal Antibodies to Three Different Preparations of Mycobacterium Paratuberculosis in Immunohistochemical Diagnosis of Johne's Disease in Cattle

1996 ◽  
Vol 8 (4) ◽  
pp. 469-473 ◽  
Author(s):  
Judith R. Stabel ◽  
Mark R. Ackermann ◽  
Jesse P. Goff
Keyword(s):  
Cell Wall ◽  
Polyclonal Antibodies ◽  
Keyhole Limpet Hemocyanin ◽  
Giant Cells ◽  
Johne's Disease ◽  
Johne’S Disease ◽  
Diagnostic Tools ◽  
Cell Wall Proteins ◽  
Incomplete Freund’S Adjuvant ◽  
Immunohistochemical Diagnosis

Polyclonal antisera were raised in rabbits against preparations of live and heat-killed Mycobac- terium paratuberculosis and cell-wall proteins of M. paratuberculosis and were evaluated as diagnostic tools in immunohistochemical staining of bovine tissue. Live preparations of M. paratuberculosis (LMp) were inoculated intraperitoneally or intravenously at 109/ml. Heat-killed M. paratuberculosis (HKMp) was prepared by treatment of bacteria at 85 C for 10 minutes. Cell-wall proteins were isolated from M. paratuberculosis and conjugated to keyhole limpet hemocyanin to improve antigenicity (KLH-CWPMp). The HKMp and KLH-CWPMp preparations were emulsified in incomplete Freund's adjuvant before subcutaneous inoculation of rabbits. Antibody titers in the terminal blood sample were higher for HKMp and KLH-CWPMp than for LMp rabbits (1:1,024 vs. 1:64). The KLH-CWPMp antibody did not cross-react with M. bovis-infected tissues. Sensitivity and specificity of immunohistochemical detection of Johne's disease (paratuberculosis) from bovine tissues was much higher for the KLH-CWPMp polyclonal antibody. Immunoreactivity of the antibody resulted in staining of bacteria in the cytoplasm of macrophages, mononuclear giant cells, and extracellular bacteria in both intestine and lymph node.

scholarly journals MiR-365a-3p-Mediated Regulation of HELLS/GLUT1 Axis Suppresses Aerobic Glycolysis and Gastric Cancer Growth

2021 ◽  
Vol 11 ◽  
Author(s):  
Rui Yang ◽  
Gen Liu ◽  
Limin Han ◽  
Yuheng Qiu ◽  
Lulin Wang ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Poor Prognosis ◽  
Bioinformatics Analysis ◽  
Aerobic Glycolysis ◽  
Metabolic Reprogramming ◽  
Cancer Growth ◽  
The Novel ◽  
The Third ◽  
Cycle Arrest ◽  
Glycolytic Activity

Gastric cancer (GC) is a common and invasive malignancy, which lacks effective treatment and is the third main reason of cancer death. Metabolic reprogramming is one of the main reasons that GC is difficult to treat in various environments. Particularly, abnormal glycolytic activity is the most common way of metabolism reprogramming in cancer cells. Numerous studies have shown that microRNAs play important roles in reprogramming glucose metabolism. Here, we found a microRNA-miR-365a-3p, was significantly downregulated in GC according to bioinformatics analysis. Low expression of miR-365a-3p correlated with poor prognosis of GC patients. Overexpression of miR-365a-3p in GC cells significantly inhibited cell proliferation by inducing cell cycle arrest at G1 phase. Notably, miR-365a-3p induced downregulation of HELLS through binding to its 3′ untranslated region (UTR). Additionally, we found that miR-365a-3p suppressed aerobic glycolysis by inhibiting HELLS/GLUT1 axis. Lastly, we shown that overexpression of miR-365a-3p significantly inhibited tumor growth in nude mice. Conversely, Reconstituted the expression of HELLS rescued the suppressive effects of miR-365a-3p. Our data collectively indicated that miR-365a-3p functioned as a tumor suppressor in GC through downregulating HELLS. Therefore, targeting of the novel miR-365a-3p/HELLS axis could be a potentially effective therapeutic approach for GC.

scholarly journals Synthetic Peptides as Immunogen in the Production of IgY Anti-C-Myc Antibody in Local Chicken (Gallus Gallus)

2019 ◽  
Vol 1 (1) ◽  
pp. 34-39
Author(s):  
Salomo Hutahaean
Keyword(s):  
Synthetic Peptide ◽  
Synthetic Peptides ◽  
Gallus Gallus ◽  
Chicken Breast ◽  
Distilled Water ◽  
Booster Injection ◽  
The Third ◽  
Local Chicken

Experiments conducted to determine the effect of doses of immunogen on the levels of IgY c-Myc antibody in chickens. To generate antibody, we used a synthetic peptide as an immunogen, which is developed from the epitope of the c-Myc protein. Hens were immunized a week after their first eggs were produced. Antigen diluted in double distilled water and emulsified with Freund 's Complete Adjuvant. The suspension was injected into the area of the chicken breast subcutaneously with a dose of 0, 0.02, 0.04, 0.06, 0.08, 0.1, 0.12, 0.14, or 0.16 mg per hen. Booster injection performed on days 10, 20, and 30 with the same volume of emulsion, but using Incomplete Freund 's Adjuvant and the amount of antigen was half of the amount of the first Immunization. Extraction of IgY from eggs was carried out using kits from Gallus Immunotech. The level of IgY was quantified using a spectrophotometer where the absorbance was read at 280 nm. The results showed that after the first immunization IgY content of the egg were reached the level of >5 mg/ml yolk when the dose of immunogen was above 0.1 mg/hen. The level was higher in eggs produced after the third booster ( >8 mg/ml yolk), but it is lower in all groups treated with immunogen above 0.1 mg/hen. We concluded, immunogen dosage of 0.1 mg/hen was optimum in the production of chicken IgY anti-c-Myc.

scholarly journals Peculiarities of Primary MARTX Cytotoxin Structure in Non-Toxigenic Vibrio cholerae strains of Different serogroups

2018 ◽  
pp. 73-77
Author(s):  
E. V. Monakhova ◽  
I. V. Arkhangel’skaya ◽  
R. V. Pisanov ◽  
S. V. Titova
Keyword(s):  
Vibrio Cholerae ◽  
Bioinformatics Analysis ◽  
Mechanisms Of Action ◽  
Gene Identification ◽  
Multiple Alleles ◽  
The Third ◽  
Environmental Objects ◽  
Miseq Platform ◽  
Adaptation Factor ◽  
Altered Proteins

Objective of the investigation was a comparative bioinformatics analysis of rtxA gene translation products  of Vibrio  cholerae strains  isolated  from  patients  and  environmental  objects. Materials and methods. 32 Vibrio cholerae strains from the Rostov-on-Don Research Anti-Plague Institute collection were used. DNA sequencing was conducted  on  the  MiSeq  platform  (Illumina),  gene  identification  and  analysis  was  carried  out  by  means  of  BioEdit 7.2.5, BLASTN 2.2.29, Blastp, Vector NTI Advance 11 software programs. Results and conclusions. The rtxA genes of the studied strains were represented by multiple alleles. AlignX-analysis of their deduced products divided 32 proteins into 3 separate clusters. The first one included proteins of O1 and nonO1/nonO139 strains similar to the prototype, the second – nonO1/nonO139 only, the third – O139. Blastp-analysis revealed that the proteins of the first cluster retained all  domains  characteristic  of  MARTX  – ACD  (actin  cross-linking),  RID  (Rho  GTFase  inactivation),  CPD  (cysteine protease) and ABH (alpha-beta hydrolase) which evidences the probability of manifestation of the typical activity. In 3 strains a new Hia domain (of putative adaptation factor) was detected. The proteins of the second cluster lacked ACD but formed an additional RID; two of which lacked ABH too, but in one, VIP2 domain (of actin modification) appeared and in another – Hia. These data are in accordance with the presumption of J. Dolores, K.J.F. Satchell (2013) on the possible converting of actinomodulator MARTX into toxins with other mechanisms of action. The proteins of O139 vibrios shared all specific domains except from ACD. For a number of altered proteins revealed by us full homologues were found in NCBI gene bank, belonging to isolates from other regions of the world. The presence of similar determinants in the genomes of strains of different origin points to non-occasional character of their conservation. It is possible that certain strains “deliberately” alter those genes or switch off the synthesis of the high-molecular toxin completely with a view to energy-saving.

scholarly journals Karakteristik Mahasiswa Terhadap Pengembangan Modul Pembelajaran Kimia Berbasis STEM pada Topik Pakan Ayam Buras di Abad 21

EduKimia ◽  
2021 ◽  
Vol 3 (3) ◽  
pp. 178-184
Author(s):  
Made Sukaryawan ◽  
K Anom W ◽  
Jejem Mujamil
Keyword(s):  
21St Century ◽  
Chemistry Education ◽  
21St Century Learning ◽  
Research Data ◽  
The Third ◽  
Learning Module ◽  
Chemistry Learning ◽  
Strongly Agree ◽  
Chicken Eggs ◽  
Descriptive Method

Students need modules in 21st century learning, especially in entrepreneurship courses. The study aims to describe the characteristics of students towards the Integrated Chemistry Learning Module STEM Entrepreneurship Course in Chemistry Education Sriwijaya University, the topic of Feed for Increasing Non-Racial Chicken Eggs. This research was conducted on the third semester student in Chemistry Education Sriwijaya University with a descriptive method. The research data were collected using a valid questionnaire with a reliability coefficient of 0.925, interviews and documentation. The results of the study show that the characteristics of student are very suitable or enable or feasible to compose a learning module on the topic of Increasing Non-Racial Chicken Eggs, in the 21st century. Student responded agree and strongly agree to use this module respectively by 57.8 percent and 42.2 percent. Respondents agree and strongly agree that they are accustomed to using Android or Internet or Laptop, respectively, by 62,2 percent and 34,4 percent.

scholarly journals Molecular Characterization of Hemagglutinin-Neuraminidase Protein Virus Newcastle disease (ND) in Surabaya during 2003 and 2016

2021 ◽  
Vol 4 (2) ◽  
pp. 231
Author(s):  
Innah Wulandari ◽  
Jola Rahmahani ◽  
Indah Rahmawati ◽  
Nurvita Putih ◽  
Aisyah Azahro ◽  
...  
Keyword(s):  
Amino Acid ◽  
Newcastle Disease ◽  
Epitope Prediction ◽  
Cloacal Swab ◽  
Sequencing Analysis ◽  
Nucleotide Homology ◽  
Reverse Primer ◽  
Chicken Eggs ◽  
Sequencing Result

This study aimed to determine the mutation of amino acid, nucleotide homology, phylogenetic tree, epitope prediction of Hemagglutinin-Neuraminidase protein Newcastle Disease (ND) Virus isolated from traditional market around Surabaya. Samples were from 37 chicken with cloacal swab and one positive samples for control (LaSota). Samples were inoculated on embyonate chicken eggs and identified with HA test confirmed with HI test. Positive samples processed by PCR using forward and reverse primer with 503 bp RNA target. The PCR result then analyzed with sequencing. Result of sequencing analysis showed that theres similiarity between samples amino acid and vaccine isolate its effect the percentage of nucleotide homology and phylogenetic relation between isolate. Epitope NGAANNSGWGAPIHDPDYIGG have high immunogenic value at all of isolate which good as vaccine candidate.

scholarly journals Identification and verification of 3 key genes associated with survival and prognosis of patients with colon adenocarcinoma via integrated bioinformatics analysis

2020 ◽  
Author(s):  
Yong Liu ◽  
Chao Li ◽  
Lijin Dong ◽  
Ping Li ◽  
Xuewei Chen ◽  
...  
Keyword(s):  
Bioinformatics Analysis ◽  
Colon Adenocarcinoma ◽  
Enrichment Analysis ◽  
Hub Genes ◽  
The Third ◽  
Treatment And Prevention ◽  
The World ◽  
Differential Gene ◽  
Key Genes ◽  
Online Software

AbstractBackgroundColorectal cancer (CRC) is the third most lethal malignancy in the world, wherein colon adenocarcinoma (COAD) is the most prevalent type of CRC. Exploring biomarkers is important for the diagnosis, treatment, and prevention of COAD.MethodsWe used GEO2R and Venn online software for differential gene screening analysis. Hub genes were screened via STRING and Cytoscape, following Gene Ontology and KEGG enrichment analysis. Finally, survival analysis and expression validation were performed via UALCAN online software, real-time PCR and immunohistochemistry.ResultsIn this study, we screened 323 common differentially expressed genes from four GSE datasets. Furthermore, four hub genes were selected for survival correlation analysis and expression level verification, three of which were shown to be statistically significant.ConclusionOur study suggests that SERPINE1, SPP1 and TIMP1 may be biomarkers closely related to the prognosis of CRC patients.

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