Plasma lipid and haematological profile during transition period inMurrah buffaloes supplemented with prilled fat

Author(s):  
Shikha Sharma ◽  
Mahendra Singh ◽  
A. K. Roy ◽  
H. M. Ajitha Kumar

Fourteen apparently healthy advanced pregnant Murrah buffaloes at 35 day prepartum were either maintained as per routine management practices (control group) or fed prilled fat (treatment group) @100g/d during the prepartum and @ 150g/d for a period of 95 days during the postpartum period. Blood samples were collected at weekly intervals during different periods i.e. prepartum, day of parturition and postpartum by day 35 and thereafter at fortnightly intervals till day 120 of lactation. Haematological parameters, plasma glucose and lipid profile were analyzed by standard method of analysis. Red blood cell (RBC) number declined on the day of parturition and increased after the parturition (P<0.01) in both the groups, however RBC count varied non - significantly between the groups. Haemoglobin (Hb) level was significantly more before parturition (P< 0.05) and was low on the day of parturition. Mean corpuscular volume (MCV) and mean corpuscular haemoglobin (MCH) in the treatment group was higher (P<0.01) on day of parturition in comparison to before and after parturition. Total leukocyte count (TLC) (P<0.01) varied significantly before, during and after parturition. However, between group changes were non-significant (P>0.05). Plasma glucose increased in the treatment group (P<0.01) over the control group. Plasma cholesterol and HDL were increased in treatment group (P<0.05) as compared to control group. However, triglyceride and VLDL cholesterol levels varied non-significantly (P>0.05) between the groups. It was concluded that supplementation of prilled fat did not influence haematological parameters; however treated buffaloes had higher blood glucose, HDL and cholesterol levels.

Biomedika ◽  
2014 ◽  
Vol 6 (1) ◽  
Author(s):  
Adhimass Wicaksono ◽  
Retno Sintowati ◽  
Sa’idatul Fitriyah

The using of plant-based medicine is a popular approach to health care. A study presented by American Hearth Association showing that brown rice can lower cholesterol level in bood serum. To know the benefi ts of Angkak’s steeping water to decrease serum cholesterol levels in mice and determine the effects of Angkak’s steeping water to decrease serum cholesterol levels in rats compared with simvastatin. This study used an experimental research design, and using pre and post test control group design, performed in the laboratory of biomedical III FK UMS on November 1, 2012 to date of January 1, 2013. The purposive sampling technique, sampel determinaton using formula Federer by the number of sample of 30 male white rats wistar strain. After the data is collected, analyzed using the Shapiro-Wilk test, ANOVA, LSD, and the unpaired t test. The result of this study demnstrate hypothesis testing using ANOVA test with signifi cant level 95% of the negative control group, the treatment group a dose of 1, 2, and 3, it was found p<0,05 were signifi cantly different meaning. In the test dose of LSD between treatment groups 2 and 3 the value of p=0,839 (p>0,05) wich means no signifi cant difference. In the unpaired T test between the treatment group a dose of 1, 2, and 3 with positive control group were signifi cant difference. The unpaired T test between the treatment group a dose of 1, 2, and 3 with white rice also found signifi cant difference. Conclusion: Angkak’s steeping water can lower serum cholesterol levels of white rat as well as the water steepimg Angkak has the same effectiveness with simvastatin to decrease serum cholesterol levels of mice.Keyword: Cholesterol, Angkak


2021 ◽  
Vol 15 (5) ◽  
pp. 1305-1308
Author(s):  
Su warsi

Background: Much alternative therapy such as cupping therapy in the community as one of the cheap treatment efforts without side effects makes the challenge and the goal for nursing staff improve nursing services. The unfinished scientific verification of cupping therapy's alternative therapy makes nurses need to prove decreased blood pressure and cholesterol levels in the elderly. Objective: Scientific proof of cholesterol and blood pressure decrease after Cupping Therapy in an elderly group in Wedomartani Village, Sleman Yogyakarta Indonesia. Method: quantitative type with pseudo experimental research design. Pre-test and Post-test nonequivalent control group. The treatment group was given wet cupping therapy, while the control group was given dry cupping. Respondents were selected using a purposive sampling technique. Blood pressure measurement uses a digital tensimeter and cholesterol measurement using a digital blood cholesterol level measuring device. Results: The elderly blood cholesterol levels after the average therapy decreased 42.89 mg/dl, whereas blood cholesterol levels in the control group decreased an average of 20.95 mg/dl. The blood pressure of the elderly systole in the intervention group after the average therapy decreased 10.74 mmHg, whereas the control group's systole blood pressure decreased an average of 1.58 mmHg. Diastole blood pressure in the treatment group decreased 3,48 mmHg, in the diastole blood pressure control group increased 5,26 mmHg. Conclusions: Wet and dry cupping are both significantly associated with decreased blood cholesterol and blood pressure levels, but wet cupping lowers blood cholesterol levels by a mean difference of 21.95 mg/dl and mean systole blood pressure of 3.74 mmHg and pressure diastole blood difference of mean 9.1 mmHg. Keyword: Blood Pressure. Cupping Therapy, Cholesterol, Elderly


2015 ◽  
Vol 4 (1) ◽  
Author(s):  
Mulfa Satria Asnel ◽  
Husnil Kadri ◽  
Dessy Arisanty

AbstrakMinyak kelapa sawit banyak mengandung asam lemak jenuh yang diduga dapat menyebabkan kolesterol dan trigliserida meningkat. Tujuan penelitian ini adalah mengetahui efek suplemen bawang putih terhadap kadar kolesterol dan trigliserida tikus galur wistar yang diberi diet tinggi minyak sawit. Ini merupakan penelitian eksperimental dengan randomized post test control group design. Subjek penelitian adalah 15 ekor tikus Wistar jantan yang dibagi menjadi tiga kelompok, yaitu kelompok diet standar ad libitum, kelompok diet tinggi minyak sawit dan kelompok perlakuan (diet tinggi minyak sawit+suplemen bawang putih). Masing-masing kelompok terdiri dari lima ekor tikus. Pemberian minyak sawit dengan kadar 3 ml/15 gram pakan standar diberikan kepada kelompok diet tinggi minyak sawit dan kelompok perlakuan selama empat minggu. Pemberian suplemen bawang putih (dosis 3,6 mg/200 mgBB tikus) diberikan kepada kelompok perlakuan selama empat minggu. Kadar kolesterol pada kelompok diet tinggi minyak sawit adalah 109 ± 2,76 mg/dl dan pada kelompok perlakuan adalah 107 ± 3,09 mg/dl. Kadar trigliserida pada kelompok diet tinggi minyak sawit adalah 84 ± 4,27 mg/dl, pada kelompok perlakuan adalah 83 ± 6,02 mg/dl. Tidak terdapat perbedaan yang bermakna kadar kolesterol dan trigliserida antara kelompok diet tinggi minyak sawit dengan kelompok perlakuan. Pada kelompok diet standar ad libitum dengan kelompok diet tinggi minyak sawit maupun antara kelompok diet standar ad libitum dengan kelompok perlakuan terdapat perbedaan yang bermakna pada kadar kolesterol dan trigliserida.Kata kunci: minyak sawit, suplemen bawang putih, kolesterol, trigliseridaAbstractPalm oil contains a lot of saturated fatty acids and it is supposed to cause increased cholesterol and triglyceride levels. The objective of this study was to determine the effect of garlic supplements on cholesterol levels and triglyceride at wistar strain rats which were fed in high diet palm oil. This study is a randomized experimental study with post-test control group design. The subjects were 15 male wistar rats were divided into three groups, standard ad libitum diet group, high diet palm oil group and the treatment group (high diet palm oil+ garlic supplement). Each group consists of five rats. Provision of palm oil with high levels of standard 3 ml/15 grams of feeding given to the high diet palm oil group and the treatment group during the four weeks. Garlic supplement (dose 3.6 mg/200 mgweight rat) was given to the treatment group for four weeks. Cholesterol levels in the high diet palm oil group was 109 ± 2,76 mg/dl in the treatment group was 107 ± 3,09 mg/dl. The levels of triglycerides in the high diet palm oil group was 84 ± 4,27 mg/dl and in the treatment group was 83 ± 6,02 mg/dl. There was no significant difference the levels of cholesterol and triglycerides between the high diet palm oil group with the treatment group. Between the standard ad libitum diet group with the high diet palm oil group and between the standard ad libitum diet group with the treatment group show there were significant differences in the levels of cholesterol and triglycerides.Keywords: palm oil, garlic supplement, cholesterol, triglyceride


Author(s):  
Yurii Boiko ◽  
Mohammed Ayat ◽  
Irina Boiko ◽  
Alexey Shandra

The search for new anti-inflammatory substances is an important area of pharmacology. Plant carotenoids have many positive biological effects and are of interest as potential anti-inflammatory agents. The aim. To study the anti-inflammatory properties of extractive carotenoids from fruits of Capsicum annuum L. on the model of adjuvant-induced inflammation. Materials and methods. The fruits of Capsicum annuum L. were used as the plant raw material. Carotenoids were obtained by the extractive method. The model of adjuvant-induced inflammation was reproduced in young female rats. The dynamics of changes in the volume of the inflammatory edema and the total number of blood leukocytes were studied. Results and discussion. A decrease in edema in nonspecific inflammation was observed on day 15 during the therapy with carotenoid extracts (Δ1.01±0.08 in treatment group; Δ1.28±0.08 in control group). For specific inflammation, edema reduction when using carotenoids occurred on day 30 (Δ0.047±0.015 in treatment group; Δ0.073±0.012 in control group). A decrease in leukocytosis occurred on days 20 and 30 of the treatment for non-specific and specific inflammation, respectively. The mechanisms of the anti-inflammatory action of carotenoids are presumably associated with the antioxidant effect and the ability to affect the production of pro-inflammatory cytokines. Conclusions. Extractive carotenoids from the fruits of Capsicum annuum L. have the anti-inflammatory activity in the case of adjuvant-induced inflammation. Treatment with Capsicum annuum L. extract reduced inflammatory edema and total leukocyte count


1970 ◽  
Vol 6 (1) ◽  
pp. 99-102 ◽  
Author(s):  
M Aktaruzzaman ◽  
J Alam ◽  
A Rahman ◽  
MM Hossain

The present study was performed in the Department of Medicine and in Veterinary clinic of Bangladesh Agricultural University (BAU), Mymensingh from January to June 2002. Myositis was produced experimentally by injecting oil of turpentine in the gluteal muscles of 4 goats of which 2 goats were given treatment with Diclofenac sodium @1mg/kg body weight (treatment group) for consecutive 3 days and remaining 2 goats were reared as control without giving treatment (control group). All the goats were observed for 7 days. Myositis was characterized by reduced appetite, increased body temperature (1-2°F), lameness, local swelling and cellular changes in blood level. The appetite in both groups reduced sharply from 1st day of myositis and improved from day 2 and became normal on 3rd day after treatment. Body temperature reduced to normal level from 2nd day after administration of diclofenac sodium but in control group, it came down in the same condition from 4th day onward. Moderate lameness was observed in all the goats of both groups. In treatment group lameness reduced completely in 100% goats after 2 consecutive days of treatment but in control group similar result was observed on day 6. Moderate local swelling was reduced to normal level in treatment group 2 days post treatment remain moderate in control group. Total leukocyte count (TLC) was increased significantly (p < 0.05) in both groups in myositic condition than in healthy condition and it came down to normal level in treatment group 3 days post treatment and in control group it remained higher. Similar changes were observed in Neutrophil count. The number of lymphocyte was reduced significantly (p < 0.05) in myositic condition than in healthy ones which again reached to normal level within 3 days of treatment. Key words: Myositis, goat, Non-steroidal anti-inflammatory drug (NSAID) DOI = 10.3329/bjvm.v6i1.1345 Bangl.  J. Vet. Med. (2008). 6 (1): 99-102


Author(s):  
A. K. Chaturvedani ◽  
Sudhir Kumar Jaiswal ◽  
Meesam Raza

The present study was conducted to assess the effects of thermal stress on the serum biochemical and haematological parameters in broiler chicken. Forty CARIBRO- Vishal broiler chicken were divided into two groups with twenty birds each. The control group was kept at room temperature and treatment group was exposed to thermal stress under 40±1 °C for 4 hours at 35 day of age. The exposure of thermal stress significantly increased (P less than 0.05) the value of serum biochemical parameters (glucose, cholesterol and triglyceride), heterophills, H/L ratios and basophills, however the value of total leukocyte, haemoglobin, haematocrit value, and monocytes decreased significantly (P less than 0.05).


2015 ◽  
Vol 18 (4) ◽  
pp. 865-872 ◽  
Author(s):  
E. Jakimiuk ◽  
J. Radwińska ◽  
A. Pomianowski ◽  
M. Woźny ◽  
K. Obremski ◽  
...  

Abstract Zearalenone (ZEN) widely contaminates animal feed of plant origin. The recommended safe concentrations of ZEN in feeds for various animal species are set mainly based on the mycotoxin’s hormonal properties (NOEL). Our growing knowledge about biologically active concentrations of ZEN, molecular mechanisms and cells/tissues targeted by ZEN indicates that the harmful effects exerted by this mycotoxin on animals may be far greater than previously believed. This experiment was performed on pre-pubertal gilts divided into a control group (n=9) and an experimental group (ZEN, n=9). The control group received placebo, whereas the experimental group was administered ZEN at a dose of 0.1 mg/kg feed (equivalent to 5 μg/kg BW/day) for 42 days. On days 14, 28 and 42 blood samples were collected from the animals to determine the concentrations of selected zearalenols, serum biochemical and haematological parameters. Conjugated ZEN was found in the blood serum of the experimental gilts. Changes in the analysed biochemical parameters included a transient increase in albumin and cholesterol levels. A statistically significant increase in the concentrations of neutrophilic and acidophilic granulocytes was observed in the white blood cell system. The results indicate that long-term per os exposure of pre-pubertal gilts to low doses of ZEN (below NOEL) has a modulatory effect on liver function and white blood cells.


2015 ◽  
Vol 3 (1) ◽  
pp. 46 ◽  
Author(s):  
Naim Alam ◽  
Md Islam ◽  
Matiar Howlader ◽  
Nasrin Lucky

<p>The effects of Oxyclozanide (Tremacid®) on some clinical (body weight) and haematological parameters (TEC, Hb, PCV, ESR and TLC) were determined in this study. Among 55 cattle, 10 were selected, which were suffering from fascioliasis and divided randomly into two groups. Each group was consisting of five cattle. One was treated with Tremacid® @ (15 mg/kg body weight). Other was kept as an infected control group. Before trials with Tremacid® initial body weight, EPG of liver fluke and hematological parameters were examined. During the experimental period the faecal samples, clinical and hematological parameters were examined on 7th, 14th, 21st and 28th days for the determination of effects of Tremacid®. A significant reduction of EPG count was found on 7th, 14th, 21st and 28th day of Tremacid® (61.87%, 71.22%, 76.98% and 84.53%) in a treated cattle group. The EPG of an untreated control group was significantly (p&lt;0.01) increased about 3.97%, 7.64%, 11.04% and 64.89% respectively. Total TEC was increased after treatment with Tremacid® and decreased in an untreated control group significantly (p&lt;0.01), Likewise, after treatment with Tremacid® Hb content and PCV were increased, and ESR was decreased. Total leukocyte count (TLC) was decreased and the body weight was increased after Tremacid® treatment in the group A. On the other hand, Hb content PCV and body weight was decreased significantly (p&lt;0.01) in the group B.</p>


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