scholarly journals PENGARUH PEMBERIAN AIR SEDUHAN BERAS YANG DIFERMENTASI OLEH MONASCUS PURPUREUS (ANGKAK) TERHADAP PENURUNAN KADAR KOLESTEROL SERUM PADA TIKUS PUTIH

Biomedika ◽  
2014 ◽  
Vol 6 (1) ◽  
Author(s):  
Adhimass Wicaksono ◽  
Retno Sintowati ◽  
Sa’idatul Fitriyah
Keyword(s):  
Treatment Group ◽  
Serum Cholesterol ◽  
Brown Rice ◽  
Negative Control ◽  
T Test ◽  
Control Group ◽  
Control Group Design ◽  
Cholesterol Levels ◽  
White Rats ◽  
Group A

The using of plant-based medicine is a popular approach to health care. A study presented by American Hearth Association showing that brown rice can lower cholesterol level in bood serum. To know the benefi ts of Angkak’s steeping water to decrease serum cholesterol levels in mice and determine the effects of Angkak’s steeping water to decrease serum cholesterol levels in rats compared with simvastatin. This study used an experimental research design, and using pre and post test control group design, performed in the laboratory of biomedical III FK UMS on November 1, 2012 to date of January 1, 2013. The purposive sampling technique, sampel determinaton using formula Federer by the number of sample of 30 male white rats wistar strain. After the data is collected, analyzed using the Shapiro-Wilk test, ANOVA, LSD, and the unpaired t test. The result of this study demnstrate hypothesis testing using ANOVA test with signifi cant level 95% of the negative control group, the treatment group a dose of 1, 2, and 3, it was found p<0,05 were signifi cantly different meaning. In the test dose of LSD between treatment groups 2 and 3 the value of p=0,839 (p>0,05) wich means no signifi cant difference. In the unpaired T test between the treatment group a dose of 1, 2, and 3 with positive control group were signifi cant difference. The unpaired T test between the treatment group a dose of 1, 2, and 3 with white rice also found signifi cant difference. Conclusion: Angkak’s steeping water can lower serum cholesterol levels of white rat as well as the water steepimg Angkak has the same effectiveness with simvastatin to decrease serum cholesterol levels of mice.Keyword: Cholesterol, Angkak

scholarly journals Pengaruh Kitosan Terhadap Jumlah Osteoklas dan Osteoblas Pada Tikus Galur Wistar Model Menopause

2017 ◽  
Vol 1 (2) ◽  
pp. 76-87
Author(s):  
Muhammad Nur
Keyword(s):  
Treatment Group ◽  
Total Sample ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Control Group Design ◽  
White Rats ◽  
Significant Difference

Chitosan is one of the most abundant and dispersed polymers in nature. It has a rapid healing effect for tissues and has an effect on bone remodeling. The purpose of this study was to prove that giving chitosan influenced the decrease of osteoclast number and increasing number of osteoblasts in female mice of wistar strain of ovariectomy menopause model.This study used a posttest only control group design. The total sample of 30 rats divided into three groups was randomized, osteoporosis was made with the menopausal model which was performed ovariectomy in the positive control group and the treatment group then in the treatment group was given chitosan extract of single dose of 45 mg / day via Nasogastric Tube (NGT). K1 was a negative control group (without ovariectomy and without chitosan), K2 was a positive control group (with no chitosan ovariectomy), K3 was the treatment group (ovariectomy and chitosan 45 mg / day).The results showed significant differences in the number of osteoblasts between K1 and K3, K2 with K3, and no significant differences were found in the K1 group with K2. While the number of osteoclasts showed that there were significant differences in K1 group with K2 and no significant difference was found in K1 group with K3, K2 with K3.Conclusions in this study showed that 45 mg / day of chitosan doses could significantly increase osteoblast counts in menopause female white rats and decrease the number of osteoclasts performed by ovariectomy with chitosan administration.

scholarly journals Antidiabetes Serbuk Umbi Tanaman Yakon (Smallanthus sonchifolius) pada Kadar Gula Darah Tikus Putih (Rattus norvegicus)

2021 ◽  
Vol 7 (1) ◽  
pp. 56-62
Author(s):  
Thatit Nurmawati ◽  
Yeni Kartika Sari ◽  
Fina Ayu Ningtyas
Keyword(s):  
Treatment Group ◽  
Blood Sugar ◽  
T Test ◽  
Control Group ◽  
Control Group Design ◽  
Glucose Levels ◽  
Smallanthus Sonchifolius ◽  
White Rats ◽  
Comparison Results ◽  
Sugar Levels

The routine and long-term use of oral antidiabetic drugs in DM patients can make the public's perception worried about the side effects of the drug. In the Yakon (SS) plant tuber there is Fructooligosacaraide (FOS) which can reduce blood sugar levels. The research objective was to determine the effect of Yakon plant tubers on blood sugar levels in white rats with type 2 diabetes mellitus model. This type of research was experimental with a pre-post test design with control-group design. Independent variable, administration of Yakon plant tuber powder, dependent variable, blood glucose levels. The sample was divided into 2 groups (dose 1, dose 2) and a control group, each of 6 rats. The mice were given a high carbohydrate diet for 9 weeks. Treatment dose 1 (100 mg / kgBB) for 3 days. Measurement of blood sugar levels using Gluco-Dr. Data analysis used Paired Sample T-test, Independent Sample T-test, and One Way Anova. The results showed that the rats in the treatment group dose 1 experienced a decrease in the average blood sugar level of 122.83 mg / dl (p 0.002) (p 0.009). This shows that there is an effect of using Yakon plant tuber powder on blood sugar levels. The results of this study were the control group and the treatment group dose 1 (Sig. (2-tailed) = 0.000, α = 0.05). Meanwhile, the comparison results showed that there was a difference between the control group and the treatment group at dose 1, Based on the above research, Yakon tubers can be used to reduce blood sugar levels.

scholarly journals PENGARUH PEMBERIAN INFUSA DAUN LABU SIAM (Sechium edule) TERHADAP PENURUNAN KADAR KOLETEROL DARAH TOTAL TIKUS PUTIH JANTAN (Rattus norvegicus)

PHARMACON ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 151
Author(s):  
Ausich Singal ◽  
Edwin De Queljoe ◽  
Paulina Yamlean
Keyword(s):  
Rattus Norvegicus ◽  
Dose Group ◽  
T Test ◽  
Blood Cholesterol ◽  
Control Group ◽  
Cholesterol Levels ◽  
White Rats ◽  
Sechium Edule ◽  
One Way Anova ◽  
Paired T Test

ABSTRACT This study aims to determine the effect of infusion of conjoined pumpkin leaves (Sechium edule) on reducing total blood cholesterol of male white rats (Rattus norvegicus). The subjects of this study were 15 male white rats with an average body weight of 200 grams which were divided into 5 groups, each group consisted of 3 mice. The method used is a laboratory experiment with a completely randomized design. The results were obtained from 2 measurements of blood cholesterol levels, namely measurements before and after treatment. The treatment begins with the provision of high-fat foods for 48 days. On the 49th day a blood cholesterol level was measured before treatment. Furthermore, treatment was given to each group, namely aquades in the negative control group, simvastatin in the positive control group, and squash leaves infusion with their respective doses in the dose group I (40%), the dose group II (20%), and the dose group III (10%). Measurement of cholesterol levels after treatment was carried out on day 54. Data were analyzed by Paired t-test and One Way ANOVA. The analysis showed that there were no significant differences between treatment groups. Judging from the change in average and percentage, 40% infusion dose of siamese pumpkin leaves gives the best reduction in cholesterol levels. Keywords: Cholesterol, pumpkin leaves, male white mouse infusion. ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh pemberian infusa daun labu siam (Sechium edule ) terhadap penuruan kolesterol darah total tikus putih jantan (Rattus norvegicus). Subjek penelitian ini berupa tikus putih jantan berjumlah 15 ekor dengan berat badan rata-rata 200 gram yang dibagi dalam 5 kelompok, masing-masing kelompok sebanyak 3 ekor. Metode yang digunakan yaitu eksperimen laboratorium dengan rancangan acak lengkap. Hasil penelitian diperoleh dari 2 kali pengukuran kadar koleterol darah yaitu pengukuran sebelum dan sesudah perlakuan. Perlakuan dimulai dengan pemberian makanan tinggi lemak selama 48 hari. Pada hari ke49 dilakukan pengukuran kadar kolesterol darah sebelum perlakuan. Selanjutnya diberikan perlakuan pada tiap kelompok yaitu aquades pada kelompok kontrol negatif, simvastatin pada kelompok kontrol positif, dan infusa daun labu siam dengan dosis masing-masing pada kelompok dosis I (40 %), kelompok dosis II (20 %), dan kelompok dosis III (10%). Pengukuran kadar kolesterol sesudah perlakuan dilakukan pada hari 54. Data diananlisis dengan Paired t-test dan One Way ANOVA. Hasil analisa menunjukan tidak terdapat perbedaan yang bermakna antar kelompok perlakuan. Dilihat dari perubahan rerataan dan presentase, dosis infusa  40% daun labu siam memberikan penurunan kadar koleterol terbaik. Kata kunci : Infusa daun Labu siam, kolesterol, tikus putih jantan.

scholarly journals PENGARUH PELATIHAN THREE CORNER DRILL TERHADAP PENINGKATAN KELINCAHAN DAN POWER

2018 ◽  
Vol 6 (3) ◽  
Author(s):  
I Gede Agunk Teddy Pratama ◽  
Made Budiawan ◽  
I Nyoman Sudarmada
Keyword(s):  
Treatment Group ◽  
Muscle Power ◽  
T Test ◽  
Training Effect ◽  
Control Group ◽  
Test Results ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
Quasi Experimental

Penelitian ini bertujuan untuk mengetahui pengaruh pelatihan three corner drill terhadap kelincahan dan power otot tungkai. Jenis penelitian adalah quasi experimental dengan rancangan the non-randomized pretest-posttest control group design. Subjek penelitian yang digunakan siswa putra peserta ektrakurikuler sepak bola SMPN 2 Pekutatan. Data post-test kelincahan dan power otot tungkai pada kelompok perlakuan dan kelompok kontrol dianalisis dengan uji-t independent pada taraf signifikansi (α) 0,05 dengan bantuan program SPSS 16.0. Berdasarkan hasil uji-t independent didapatkan hasil : (1) untuk variabel kelincahan, hasil perbandingan kelompok perlakuan dan kontrol didapatkan nilai thitung = -2,948 , dengan nilai signifikansi 0,009, (2) untuk variabel power, hasil perbandingan kelompok perlakuan dan kontrol didapatkan nilai thitung = 3,176, dengan nilai signifikansi 0,005. Nilai signifikansi hitung lebih kecil dari nilai α (Sig < 0,05), dengan demikian hipotesis penelitian “pelatihan three corner drill berpengaruh terhadap peningkatan kelincahan dan power otot tungkai pada siswa putra peserta ektrakurikuler sepak bola SMPN 2 Pekutatan” diterima. Dari hasil analisis data dan pembahasan disimpulkan bahwa : (1) Pelatihan three corner drill berpengaruh terhadap peningkatan kelincahan pada siswa putra peserta ektrakurikuler sepak bola SMP Negeri 2 Pekutatan (2) Pelatihan three corner drill berpengaruh terhadap peningkatan power otot tungkai pada siswa putra peserta ektrakurikuler sepak bola SMP Negeri 2 Pekutatan. Kata Kunci : kelincahan, daya ledak, three corner drill The purpose of this research was to find out the effect of three corner drill training toward the increased agility and power muscle of legs. The type of research was a quasi-experimental and the design of research was the non-randomized pretest-posttest control group design. The subjects were man’s student football extracurricular participants of SMPN 2 Pekutatan. Agility and muscle power of legs posttest to the treatment group and the control group were analyzed by independent t-test at significance 0,05 with SPSS 16.0. Based on independent t-test results were showed : (1) to the agility variable, the comparison of the treatment and the control group values obtained t = -2,948, with a significance value of 0,009, (2) to muscle power of legs variable, the comparison of the treatment and the control group values obtained t = 3,176, with a significance value of 0,005. Calculated significance value smaller than the value of 0,05 (Sig < 0.05), thus the research hypothesis "three corner drill training affect the increased agility and muscle power of legs in football extracurricular participants of SMPN 2 Pekutatan " was received. From the analyzed and discussion were concluded that : (1) three corner drill training effect the increased agility in football extracurricular participants of SMPN 2 Pekutatan, (2) three corner drill training effect the increased muscle power of legs in football extracurricular participants of SMPN 2 Pekutatan. keyword : Agility, power, three corner drill

scholarly journals PENGARUH PEMBERIAN JUS CAMPURAN BELIMBING (Averhoa carambola linn) DAN MENTIMUN (Cucumis sativus linn) TERHADAP TEKANAN DARAH PASIEN HIPERTENSI RAWAT JALAN DI RUMAH SAKIT UMUM DAERAH KOTA MATARAM

2019 ◽  
Vol 4 (1) ◽  
pp. 40
Author(s):  
Devi Rahma ◽  
Suhaema Suhaema ◽  
Fifi Luthfiyah ◽  
Made Darawati
Keyword(s):  
Blood Pressure ◽  
Treatment Group ◽  
Antihypertensive Drugs ◽  
Vegetable Consumption ◽  
Research Result ◽  
T Test ◽  
Control Group ◽  
High Potassium ◽  
Control Group Design ◽  
Average Blood Pressure

Background. Hypertension can be a risk factor for stroke, coronary heart disease, heart failure and kidney failure and is known as the thesilent killer. The prevalence of hypertension in West Nusa Tenggara Province is 24.3% (Riskesdas, 2013). Medical Record Data of Mataram City Public Hospital, hypertension became the first of the top 10 outpatient diseases in 2015. The only treatment for non-pharmacological hypertension was by increasing fruit and vegetable consumption due to high potassium content. Research Methods. The type of this research is experimentation with Randomized Control Trial (RCT) design with Pretest-Posttest with Control Group design. The number of subjects is 20 people obtained from the formula Lemeshow (1997). Both groups continued to take antihypertensive drugs, but the treatment group was given 250 ml of starfruit juice and cucumber juice for 7 days. The statistical analysis used was the paired t-test and independent t-test. Research Result. The average blood pressure before the study in the treatment group was 150/91 mmHg, in the control group it was 142/83 mmHg. The average blood pressure after the study for the treatment group was 132/81 mmHg, while the control group was 144/81 mmHg. There was a decrease in blood pressure in the treatment group after the intervention was 18 mmHg for systolic (p = 0.000) and 10 mmHg for diastolic (p = 0.004), but there was no decrease in blood pressure both systolic and diastolic in the control group. Conclusion. Giving a mixture of star fruit and cucumber juice affects the decrease in systolic and diastolic blood pressure.

scholarly journals Potensi Ekstrak Daun Kelor (Moringa Oleifera) sebagai Hepatoprotektor pada Tikus Putih (Rattus novergicus) yang Diinduksi Parasetamol Dosis toksik

2018 ◽  
Vol 5 (1) ◽  
pp. 58
Author(s):  
Noer Kumala Indahsari ◽  
Masfufatun Masfufatun ◽  
Emilia Devi D.R
Keyword(s):  
Treatment Group ◽  
Moringa Oleifera ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Flavonoid Compound ◽  
Control Group ◽  
High Dose ◽  
Control Group Design ◽  
Post Test

Moringa Oleifera is a plant that contains chemical compounds that are useful, such as flavonoids. The ability of this flavonoid compound that can capture free radicals cause damage and hepatoprotektan hepar. Purpose of study was to determined levels of Moringa leaf extract which can overcome the effects of liver damage caused by toxic doses of paracetamol through MDA, SGOT and SGPT Method used in this laboratory experimental study is a Randomized Post Test Only Control Group Design with the following stages: 1. Moringa Leaf Extraction with Ethanol 96%; Try 2.Preparasi animals, 3. Treatment of Animals Try the extract of leaves of Moringa 3 dose is: 250mg / 200BB rat (dose of A), 500mg / 200BB mice (dose B), 1000mg / 200BB mice (dose C) for 14 days in combination with paracetamol 2 g / 200BB mice, compared to the negative control group (group given just paracetamol 2 g / 200BB rat) and the positive control group (the group who were given regular feed) for 14 days.Results : turned out to be no difference in the reduction in SGOT levels are statistically significant between the negative control group with high-dose treatment group ie the dose C with =0,016 smaller than 0.05, whereas a decrease in ALT levels were significantly decreased in the treatment group high dose is the dose C with =0,009 smaller than 0.05. While MDA group treated with the negative control group experienced an overall decline for the dose A with =0,05, dose B with =0,0011 and dose C with =0,001. Conclusion of this study showed that the extract of Moringa leaves can be potentially as an antioxidant in all doses at once can be as hepatoprotektor at high doses is 1000mg / 200BB Rattus Novergicus.

scholarly journals PELATIHAN PUKULAN BAYANGAN LEBIH BAIK DARI PADA PUKULAN BIASA DALAM MENINGKATKAN VO2MAX DAN DAYA TAHAN OTOT LENGAN PADA ATLET TINJU AMATIR KABUPATEN LOMBOK TENGAH (NTB)

2019 ◽  
Author(s):  
Khaerul Anam ◽  
I P G Adiatmika ◽  
I Putu Adiartha Griadhi ◽  
I Made Muliarta ◽  
Luh Putu Ratna Sundari ◽  
...  
Keyword(s):  
Treatment Group ◽  
Anaerobic Capacity ◽  
T Test ◽  
Control Group ◽  
Research Subjects ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
Arm Muscles ◽  
Test Control

Boxing is the basic gesture for all martial arts, because all of them definitely use the shadow shot and common shot. These two types of shots are related each other because anaerobic capacity must go along with aerobic capacity.To attain the proper knowledge about the method of shadow shot and common shot in increasing VO2max for the endurance of arm muscles for boxer athletes in Central Lombok Regency. Research was experimental with pre-test and post-test control group design, this research wasconducted in Pertina Lombok Tengah Regency. The research subjects involved 15 people divided into two groups, 7 and 8 samples respectively. The control group given only shadow shot while treatment group given common shot. This training held 3 times a week in six weeks for both groups. The analyses data with independent t-test. Researcher found The endurance of arm muscles (p<0,05) that training in twu group his’not significant to VO2max (p>0,05).Keywords: Shadow blows, regular blows, Boxing, VO2max and Endurance of arm Muscl

scholarly journals Efficacy of Topical Coffee Paste to Collagen Density in Wistar Rats Incision Wound

2018 ◽  
Vol 2 (1) ◽  
pp. 12-15
Author(s):  
Debby Handayati Harahap ◽  
Fifa Argentina ◽  
Yuli Kurniawati ◽  
Citra Tresna Murti ◽  
Yudha Dwi Satrio
Keyword(s):  
Vitamin C ◽  
Design Research ◽  
Negative Control Group ◽  
Negative Control ◽  
T Test ◽  
Control Group ◽  
Average Difference ◽  
White Rats ◽  
Collagen Density ◽  
Animal House

Background :Collagen plays a very important role in humans at every stage of wound healing. Coffee contains many antioxidants that stimulate the formation of collagen dermis by increasing the production of Tissue Inhibitor of Matrix Metalloproteinas-1 in the dermis that serves to inhibit the breakdown of collagen. This study aims to determine the efficacy of topical pasta coffee to collagen density. Methods: Experimental Laboratories Study with pre and post test controlled group design research was undertaken from September to November 2017 at Animal House and Dyatnitalis Laboratory, Palembang. 30 male white rats of wistar strain were divided into 5 groups then performed 2 cm dermal incision and giving various concentration of coffee paste. On the day 0 and 5th after coffee paste, the percentage of collagen density is checked. The efficacy of coffee paste was analyzed by Paired T Test/Wilcoxon while the comparison of efficacy  was analyzed by Independent T Test / Mann Whitney then continued with One Way ANOVA Test and Tukey test. Data analysis using SPSS version 18.0. Results: There was an average difference of percentage level of collagen density before and after vitamin C (p = 0,003), coffee paste 20% (p = 0,043), coffee paste 40% (p = 0,045) and coffee paste 80% (p = 0,011) . In addition, there was an average difference of percentage of collagen density between negative control group with positive control group and 40% coffee paste. From the results of Post Hoc test results showed that there was a difference of percentage of collagen density of negative control group with coffee paste group 40%. Conclusion: There is efficacy of topical coffee paste on collagen density in various concentrations where the highest increase of collagen density is 246,96% after coffee paste 40%. Keyword: Coffee Pasta, Collagen, Injured Incision, Vitamin C

scholarly journals Efek Suplemen Bawang Putih terhadap Kadar Kolesterol dan Trigliserida pada Tikus Wistar yang Diberi Diet Tinggi Minyak Sawit

2015 ◽  
Vol 4 (1) ◽  
Author(s):  
Mulfa Satria Asnel ◽  
Husnil Kadri ◽  
Dessy Arisanty
Keyword(s):  
Treatment Group ◽  
Palm Oil ◽  
Diet Group ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Cholesterol Levels ◽  
Post Test ◽  
Ad Libitum ◽  
Test Control

AbstrakMinyak kelapa sawit banyak mengandung asam lemak jenuh yang diduga dapat menyebabkan kolesterol dan trigliserida meningkat. Tujuan penelitian ini adalah mengetahui efek suplemen bawang putih terhadap kadar kolesterol dan trigliserida tikus galur wistar yang diberi diet tinggi minyak sawit. Ini merupakan penelitian eksperimental dengan randomized post test control group design. Subjek penelitian adalah 15 ekor tikus Wistar jantan yang dibagi menjadi tiga kelompok, yaitu kelompok diet standar ad libitum, kelompok diet tinggi minyak sawit dan kelompok perlakuan (diet tinggi minyak sawit+suplemen bawang putih). Masing-masing kelompok terdiri dari lima ekor tikus. Pemberian minyak sawit dengan kadar 3 ml/15 gram pakan standar diberikan kepada kelompok diet tinggi minyak sawit dan kelompok perlakuan selama empat minggu. Pemberian suplemen bawang putih (dosis 3,6 mg/200 mgBB tikus) diberikan kepada kelompok perlakuan selama empat minggu. Kadar kolesterol pada kelompok diet tinggi minyak sawit adalah 109 ± 2,76 mg/dl dan pada kelompok perlakuan adalah 107 ± 3,09 mg/dl. Kadar trigliserida pada kelompok diet tinggi minyak sawit adalah 84 ± 4,27 mg/dl, pada kelompok perlakuan adalah 83 ± 6,02 mg/dl. Tidak terdapat perbedaan yang bermakna kadar kolesterol dan trigliserida antara kelompok diet tinggi minyak sawit dengan kelompok perlakuan. Pada kelompok diet standar ad libitum dengan kelompok diet tinggi minyak sawit maupun antara kelompok diet standar ad libitum dengan kelompok perlakuan terdapat perbedaan yang bermakna pada kadar kolesterol dan trigliserida.Kata kunci: minyak sawit, suplemen bawang putih, kolesterol, trigliseridaAbstractPalm oil contains a lot of saturated fatty acids and it is supposed to cause increased cholesterol and triglyceride levels. The objective of this study was to determine the effect of garlic supplements on cholesterol levels and triglyceride at wistar strain rats which were fed in high diet palm oil. This study is a randomized experimental study with post-test control group design. The subjects were 15 male wistar rats were divided into three groups, standard ad libitum diet group, high diet palm oil group and the treatment group (high diet palm oil+ garlic supplement). Each group consists of five rats. Provision of palm oil with high levels of standard 3 ml/15 grams of feeding given to the high diet palm oil group and the treatment group during the four weeks. Garlic supplement (dose 3.6 mg/200 mgweight rat) was given to the treatment group for four weeks. Cholesterol levels in the high diet palm oil group was 109 ± 2,76 mg/dl in the treatment group was 107 ± 3,09 mg/dl. The levels of triglycerides in the high diet palm oil group was 84 ± 4,27 mg/dl and in the treatment group was 83 ± 6,02 mg/dl. There was no significant difference the levels of cholesterol and triglycerides between the high diet palm oil group with the treatment group. Between the standard ad libitum diet group with the high diet palm oil group and between the standard ad libitum diet group with the treatment group show there were significant differences in the levels of cholesterol and triglycerides.Keywords: palm oil, garlic supplement, cholesterol, triglyceride

scholarly journals Pengaruh Pemberian Susu Coklat Terhadap Kadar F2Isoprostan pada Siswa di Pusat Pendidikan dan Latihan Olahraga Pelajar (PPLP) Sumatera Barat

2015 ◽  
Vol 4 (3) ◽  
Author(s):  
Dezi Ilham ◽  
Afriwardi Afriwardi ◽  
Eti Yerizel
Keyword(s):  
Free Radicals ◽  
Treatment Group ◽  
Physical Exercise ◽  
T Test ◽  
The Body ◽  
Control Group ◽  
Milk Chocolate ◽  
Control Group Design ◽  
Paired Sample ◽  
Before And After

Abstrak Latihan fisik yang berlebihan dapat meningkatkan produksi radikal bebas di dalam tubuh. Peningkatan radikalbebas ini kalau tidak dinetralisasi oleh antioksidan di dalam tubuh, maka terjadilah stres oksidatif yang dapatmenyebabkan terjadinya peroksidasi lipid, sehingga menghasilkan produk senyawa F2-isoprostan yang dapatberdampak terhadap berbagai masalah kesehatan. Tujuan penelitian ini adalah untuk menentukan pengaruhpemberian susu coklat terhadap kadar F2-isoprostan. Penelitian ini merupakan eksperimental pre and post test controlgrup design terhadap 36 siswa atlet, yang memenuhi kriteria inklusi dan ekslusi. Subjek dibagi dua kelompok yaituyang diberikan perlakuan susu coklat dan kontrol susu putih. Susu diminum setelah latihan fisik rutin atlet sebanyaksatu kali sehari selama 15 hari. Kadar F2-isoprostan dinilai sebelum dan sesudah secara ELISA. Data dianalisisdengan paired sample t-Test, bermakna bila p<0,05. Hasil penelitian didapatkan rerata kadar F2-isoprostan sebelumperlakuan pada kelompok susu coklat adalah 65,98±14,61 pg/ml dan sesudah perlakuan adalah 45,55±13,74 pg/ml.Rerata kadar F2-isoprostan sebelum perlakuan pada kelompok kontrol susu putih adalah 54,24±20,44 pg/ml dansesudah perlakuan adalah 48,80±20,23 pg/ml. Kesimpulan yang diperoleh ada pengaruh yang bermakna dari reratakadar F2-isoprostan sebelum dan sesudah perlakuan baik pada kelompok perlakuan susu coklat dan kelompok kontrolsusu putih.Kata kunci: latihan fisik, radikal bebas, F2-isoprostan, susu coklatAbstract Excessive physical exercise can increase the production of free radicals in the body. The increase in freeradicals if not neutralized by antioxidants in the body and it caused oxidative stress which can cause lipid peroxidation,resulting in F2-isoprostane product compounds that can affect a variety of health problems. The objective of this studywas to determine the influence of milk chocolate on F2-isoprostane levels. This study was an experimental pre andpost test control group design to the 36 student athletes, who meet the inclusion and exclusion criteria. The subjectswere divided in two groups: the treatment given milk chocolate and white milk is given control, drunk after a routinephysical exercise athlete, once a day for 15 days. F2-isoprostane levels were assessed before (pre) and after (post) byELISA, the data were analyzed by Paired Sample t-Test, significant if p <0.05. The results showed average levels ofF2-isoprostane before treatment group was 65.98 ± chocolate milk 14.61 pg / ml and after treatment was 45.55 ±13.74 pg / ml. Average F2-isoprostane levels before treatment in the control group was 54.24 ± white milk 20.44 pg /ml and after treatment was 48.80 ± 20.23 pg / ml. There is a significant influence on the mean levels of F2-isoprostaneboth before and after treatment in the treatment group and the control group of milk chocolate and white milkKeywords: physical exercise, free radicals, f2-isoprostane, milk chocolate

Sign in / Sign up

Export Citation Format

Share Document