Effect of saline water on rumen fermentation and serum profile in Murrah male calves

2017 ◽  
Author(s):  
Preeti . ◽  
Neelam Kewalramani ◽  
S. S. Kundu ◽  
Amit Sharma
Keyword(s):  
Water Intake ◽  
Saline Water ◽  
Block Design ◽  
Experimental Period ◽  
Blood Parameters ◽  
Ammonia Nitrogen ◽  
Rumen Fermentation ◽  
Monthly Interval ◽  
Blood Profile ◽  
Randomized Block Design

Twenty growing male Murrah calves, with average body weight (kg) 220±5, were randomly assigned to five treatments of water containing total dissolved solids (TDS; ppm) viz CON=500; T1=2,000; T2=4,000; T3=6,000 and T4=8,000 at National Dairy Research Institute (NDRI), Karnal India. Animals were arranged in a randomized block design with 120-day experimental period. Feed and water intake were measured daily, rumen fermentation parameters and blood profile were evaluated at monthly interval. Water intake was apparently higher in T1 (17.46 l/day) and T2 (16.77 l/day) as compared to CON (control; 14.07 l/day) but did not differ significantly. However, water intake was lower (P less than 0.05) in T3 (10.40 l/day) and T4 (8.18 l/day) as compared to group T1, T2 and T3CON. Except ammonia nitrogen which was decreased with increasing TDS of water, all other rumen liquor parameters were found similar. In blood parameters, there was significant decrease (P less than 0.05) in plasma glucose, total protein, and albumin while there was significant increase (P less than 0.05), in AST and ALT with increasing TDS of water. Blood Urea Nitrogen, creatinine, uric acid were not affected. On the basis of above results it was inferred that water intake was decreased at higher TDS (above 4000 ppm)

PSX-B-1 Effect of exposure time and dose of effective microorganisms (EM®) on feed in vitro rumen fermentation and digestibility

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 455-457
Author(s):  
Alain Miranda-Figueroa ◽  
Carlos Gutiérrez_Aguilar ◽  
Manuel González-Ronquillo ◽  
Atmir Romero-Pérez ◽  
Claudia Cecilia Márquez-Mota ◽  
...  
Keyword(s):  
Exposure Time ◽  
Volatile Fatty Acids ◽  
Block Design ◽  
Ammonia Nitrogen ◽  
Rumen Fermentation ◽  
Effective Microorganisms ◽  
Time Interaction ◽  
Randomized Block Design ◽  
Quadratic Effects

Abstract The objective was to evaluate the effect of feed inoculation with effective microorganisms (EM®) (mainly containing Lactobacillus spp.,Rhodopseudomona palustrisand Saccharomyces cerevisiae) on rumen fermentation using in vitrogas production technique. We hypothesized that increasing doses and allowing exposure of EM® for up to 48 hours, would improve digestibility and rumen fermentation. The experimental design was a 4×4 completely randomized block design including 4 EM® levels [(0(EM0), 0.5(EM0.5), 1.0 (EM1) and 1.5 (EM1.5) mL EM® / kg DM] and 4 preincubation times [0 (T0), 12(T12), 24(T24), 48 (T48) h], with four repetitions per treatment. Treatments were evaluated using 100ml glass bottles with 0.5g of the diet (20% corn stover, 20% oat hay, 48.8% ground corn, 7% molasses, 1.2% urea, 1% soybean meal, 0.9% mineral premix,1.1% salt, dry matter basis) incubated with sheep ruminal fluid in 3 different occasions. Data were analyzed with PROC MIXED of SAS and orthogonal contrasts to determine the linear and quadratic effects of EM dose and exposure time. Interaction (P < 0.05) of EM x T was observed for in vitrodry matter digestibility (IVDMD), maximum gas volume (Vmax), total volatile fatty acids (VFA), acetate (ACE), propionate (PROP), butyrate (BUT) and ammonia-nitrogen (NH3), IVDMD was higher (P < 0.01, 4.8 and 3.72%) for T48EM1.5 than T12EM0 and T0EM0, PROP was higher (P < 0.05) for T48EM0, T48EM1 and 1.5 than T12EM0. The ACE:PROP ratio was higher (P < 0.05, 17.2%) for T12EM0 than T48EM1.5. IVDMD, PROP and NH3 linearly increased (P < 0.01) with increasing exposure time. EM levels have a quadratic effect (P < 0.01) with maximum response at EM0.5. It was concluded that the addition of 0.5 to 1.5 mL/kg DM of EM® to a sheep diet and increasing preincubation time, up to 48h, improve feed fermentation and digestibility.Project was supported by UNAM, DGAPA, PAPIIT (IT202120).

scholarly journals Evaluating dietary acidifiers as alternatives for conventional feed-based antibiotics in nursery pig diets

2021 ◽  
Author(s):  
Payton L Dahmer ◽  
Cassandra K Jones
Keyword(s):  
Block Design ◽  
Average Daily Gain ◽  
Random Effect ◽  
Dietary Treatment ◽  
Experimental Period ◽  
Blood Parameters ◽  
Negative Control ◽  
Experimental Unit ◽  
Randomized Block Design ◽  
Nursery Pig

Abstract A total of 360 weanling pigs (DNA 200 × 400; initially 9.7 ± 0.23 kg BW) were used in a 21-d experiment with 6 pigs/pen, 10 replicate pens/treatment, and 2 separate nursery rooms, each with 30 pens. Pigs were weighed and allotted to pens based on BW in a completely randomized block design to one of 6 treatment diets: 1) Negative control (no organic acids or antibiotics) and the control with 2) 0.25% Acidifier A; 3) 0.3% Acidifier B; 4) 0.5% Acidifier C); 5) 50 g/t Carbadox; 6) 400 g/t Chlortetracycline. Upon weaning, a common diet with no antibiotics or additives was fed for 21 d (phases 1 and 2; d -21 to d 0), followed by a 21 d experimental period (phase 3; d 0 to d 21) where treatment diets were fed. Pigs and feeders were individually weighed on a weekly basis to calculate average daily gain (ADG), average daily feed intake (ADFI) and feed efficiency (G:F). Data were analyzed using the PROC GLIMMIX procedure of SAS (v 9.4, SAS Inst., Cary, NC) with pen as the experimental unit, treatment as a fixed effect and room as a random effect. Dietary treatment had a significant impact (P < 0.05) on ADG, ADFI and G:F each week and for the overall experimental period (d 0 to 21). Specifically, from d 0 to 7, pigs fed CTC had increased (P = 0.001) ADG compared to those fed Acidifier B, Acidifier C and Carbadox, while pigs fed the negative control and Acidifier A diets were intermediate. Additionally, pigs fed the CTC diet had improved (P = 0.0002) ADFI when compared to all other treatments. From d 7 to 14 and d 14 to 21, pigs fed the Carbadox diet had decreased (P < 0.0001) ADG compared to all other treatments. During the overall period (d 0 to 21), pigs fed diets containing Carbadox had reduced ADG and ADFI (P < 0.0001), while pigs fed CTC had improved (P < 0.0001) ADG compared to all other treatments. Additionally, blood parameters, fecal consistency and fecal microbial populations were analyzed on a subset of pigs (n = 5 pigs/treatment). Dietary treatment significantly impacted (P < 0.05) concentrations of protein, globulin, phosphorus, alkaline phosphatase, and sorbitol dehydrogenase in the blood. Treatment also significantly impacted (P = 0.0005) fecal score but did not affect (P = 0.59) fecal microbial growth from d 0 to 21. In summary, CTC continues to be a valuable additive to improve performance in the nursery. Further investigation surrounding the efficacy of dietary acidifiers as antibiotic alternatives is warranted given inconclusive evidence in this study.

Effect of intraruminal infusion of urea on the response in voluntary food intake by sheep

1985 ◽  
Vol 36 (3) ◽  
pp. 483 ◽  
Author(s):  
JK Egan ◽  
PT Doyle
Keyword(s):  
Organic Matter ◽  
Food Intake ◽  
Dry Matter ◽  
Block Design ◽  
Ammonia Nitrogen ◽  
Dry Matter Intake ◽  
Plant Cell Walls ◽  
Randomized Block Design ◽  
Ad Libitum ◽  
Ad Libitum Intake

Six mature Merino sheep received three treatments in a randomized block design experiment. The treatments were: chopped oaten hay diet at 90% of ad libitum intake without urea (L); the ciet offered at the same level as for L with urea infused into the rumen at 11.5 g kg-1 dry matter intake (LU); and the diet offered at 90% of the ad libitum intake achieved with urea infused at 11.5 g kg-1 dry matter intake (HU). Sheep given HU consumed 37% more (P < 0.01) organic matter (OM) than those fed L or LU, but the apparent digestibility of OM did not vary (59.2-61.8%) between treatments. The addit onal food consumption was associated with c. 20% increase (P < 0.05) in the weight of OM in the reticulorumen and significantly higher (by 10-35%; P < 0.05) fractional outflow rates of most dietary and microbial constituents of digesta. The fractional digestion rate of potentially digestible plant cell walls was not affected by urea, but the flow of microbial non-ammonia nitrogen from the abomasum was enhanced (L, 7.0; LU, 8.2; HU, 12.5 g day-1; P < 0.05). The results of this study suggest that the stimulatory effect of urea upon food intake was associated with the provision of additional microbial protein for digestion in the intestines, rather than changes in the rate or extent of organic matter fermentation in the reticulorumen.

scholarly journals Physiological Parameters of Pigs Raised with and without Environmental Enrichment

2017 ◽  
Vol 9 (4) ◽  
pp. 176
Author(s):  
Carlos Syllas Monteiro Luz ◽  
Leonardo Atta Farias ◽  
José Luiz Leonardo Araujo Pimenta ◽  
Joisiane Da Silva Santos ◽  
Richard Átila De Sousa ◽  
...  
Keyword(s):  
Weight Gain ◽  
Environmental Enrichment ◽  
Block Design ◽  
Environmental Parameters ◽  
Well Being ◽  
Blood Parameters ◽  
Physiological Parameters ◽  
Pig Farming ◽  
Randomized Block Design ◽  
Morning Shift

Ethical and commercial issues are associated with animal welfare in pig farming, in that sense, the objective of this work was to evaluate physiological and blood parameters in addition to weight gain in pigs raised with and without sunlight and environmental enrichment (EE), as well as to correlate physiological and environmental parameters as welfare indicators. Twenty-four pigs distributed in a randomized block design with a 2 × 2 factorial arrangement were used. Blocks were represented by shifts (morning and afternoon), and factors by the presence or absence of EE and sunlight. Blood and environmental parameters of thermal comfort, in addition to weight gain, were registered. Physiological parameters showed to be shift dependent (p < 0.05), with the afternoon shift being superior when compared to the morning shift, thus indicating climate influence. Weight gain showed significant interactions (p < 0.05) in the presence and absence of sunlight and EE in the stalls. Temperature correlated positively with the physiological parameters. Moisture showed negative correlations with physiological parameters. The packed cell volume was lower (p < 0.05) in the presence of EE. The EE provides physiological well-being and weight gain for pigs in the nursery stage, especially when sunlight was absent in the stalls.

scholarly journals Physiological responses of beet plants irrigated with saline water and silicon application

2020 ◽  
Vol 11 ◽  
pp. E3113
Author(s):  
José Sebastião de Melo Filho ◽  
Toshik Iarley da Silva ◽  
Anderson Carlos de Melo Gonçalves ◽  
Leonardo Vieira de Sousa ◽  
Mario Leno Martins Véras ◽  
...  
Keyword(s):  
Stomatal Conductance ◽  
Gas Exchange ◽  
Carbon Concentration ◽  
Saline Water ◽  
Block Design ◽  
Essential Element ◽  
Net Photosynthesis ◽  
Crop Productivity ◽  
Experimental Unit ◽  
Randomized Block Design

Although not considered an essential element, silicon can be used to increase crop productivity, especially under stress conditions. In this sense, the objective was to evaluate the gas exchange of beet plants irrigated with saline water depending on the application of silicon. The experiment was conducted in a randomized block design, in a 5 x 5 factorial, referring to five levels of electrical conductivity of irrigation water (ECw): (0.5; 1.3; 3.25; 5.2 and 6.0 dS m-1) and five doses of silicon (0.00; 2.64; 9.08; 15.52 and 18.16 mL L-1), with six beet plants as an experimental unit. The effect of treatments on beet culture was evaluated at 30 and 60 days after irrigation with saline water from measurements of internal carbon concentration, stomatal conductance, net photosynthesis rate, instantaneous water use efficiency and instantaneous carboxylation efficiency using the LCpro+Sistem infrared gas analyzer (IRGA). Irrigation with saline water reduced the gas exchange of beet plants at 60 days after irrigation, but at 30 days after irrigation, the use of saline water increased stomatal conductance, transpiration rate and internal carbon concentration. The application of silicon decreased stomatal conductance, internal carbon concentration and efficiency in the use of water, but increased the rate of net photosynthesis, the rate of transpiration and instantaneous efficiency of carboxylation at 30 and 60 days after irrigation.

scholarly journals The Response of Fordhook Giant / Swisschard (Beta vulgaris var Cicla) and Mustard (Brassica Juncea) Spinach Vegetables to Irrigation with Saline Water

2020 ◽  
pp. 20-26
Author(s):  
M. V. Dlamini ◽  
M. T. Masarirambi
Keyword(s):  
Plant Height ◽  
Irrigation Water ◽  
Saline Water ◽  
Block Design ◽  
Water Source ◽  
Saline Irrigation ◽  
Randomized Block Design ◽  
Salinity Levels ◽  
Number Of Leaves ◽  
The University

Saline irrigation water is becoming an important water source as fresh water is fast becoming a scarce resource in many areas of the world, including Eswatini, especially in arid and semi-arid regions.  A study to test the response of two varieties of spinach (fordhook giant and mustard) to salinity was conducted in a field pot experiment at the Faculty of Agriculture at the Luyengo Campus of the University of Eswatini.  The treatments were laid in a randomized block design (RCBD).  The experiment consisted of four treatments, each replicated twelve times.  Treatments were salinity levels of 0.0 dS/m, 1.5 dS/m, 2.0 dS/m and 3.5 dS/m.  All the treatments were subjected to similar agronomic practices. Spinach was grown and observed for a period of five weeks.  Plant height was measured and the number of leaves counted weekly throughout the experiment. Significant differences (P < 0.05) between salinity treatments were obtained for plant height beginning in week 2 but were more pronounced in week 3, 4 and week 5.  No significant differences were obtained for the number of leaves.  There were however, clear significant differences between spinach irrigated with none saline irrigation water compared to saline irrigation water.   It was concluded that irrigating spinach with saline water of more than 2.0 dS/m drastically reduce plant growth but not the number of leaves under the conditions of the experiment.

scholarly journals GAS EXCHANGE, CHLOROPLAST PIGMENTS AND GROWTH OF PASSION FRUIT CULTIVATED WITH SALINE WATER AND POTASSIUM FERTILIZATION 1

2020 ◽  
Vol 33 (1) ◽  
pp. 184-194
Author(s):  
GEOVANI SOARES DE LIMA ◽  
COSMO GUSTAVO JACOME FERNANDES ◽  
LAURIANE ALMEIDA DOS ANJOS SOARES ◽  
HANS RAJ GHEYI ◽  
PEDRO DANTAS FERNANDES
Keyword(s):  
Gas Exchange ◽  
Saline Water ◽  
Block Design ◽  
Co2 Assimilation ◽  
Passion Fruit ◽  
Water Salinity ◽  
Chloroplast Pigments ◽  
Potassium Fertilization ◽  
Randomized Block Design ◽  
Fruit Plants

ABSTRACT The objective of this study was to evaluate the gas exchange, chloroplast pigments and growth of ‘BRS Rubi do Cerrado’ passion fruit as a function of irrigation with saline water and potassium fertilization in the seedling formation stage. The experiment was conducted under greenhouse conditions in the municipality of Pombal-PB, Brazil. A randomized block design was used in 5 x 2 factorial scheme, corresponding to five levels of water electrical conductivity - ECw (0.3, 1.1, 1.9, 2.7 and 3.5 dS m-1) and two doses of potassium - KD (50 and 100% of the recommendation), with four replicates and two plants per plot. Water salinity from 0.3 dS m-1 reduced the stomatal opening, transpiration, CO2 assimilation and inhibited the growth of ‘BRS Rubi do Cerrado’ passion fruit plants, at 40 days after sowing. There was no CO2 restriction in the substomatal cavity of passion fruit plants grown under water salinity from 0.3 dS m-1. Potassium dose of 150 mg kg-1 of soil, corresponding to 100%, intensified the effect of salt stress on the assimilation rate and instantaneous carboxylation efficiency in 'BRS Rubi do Cerrado' passion fruit. There was interaction between water salinity levels and potassium doses for the chlorophyll a and b contents of 'BRS Rubi do Cerrado' passion fruit.

scholarly journals Organic fertilization to attenuate water salinity effect on papaya growth

2019 ◽  
Vol 23 (2) ◽  
pp. 79-83
Author(s):  
Mônica S. da S. Sousa ◽  
Vera L. A. de Lima ◽  
Marcos E. B. Brito ◽  
Luderlândio de A. Silva ◽  
Rômulo C. L. Moreira ◽  
...  
Keyword(s):  
Irrigation Water ◽  
Saline Water ◽  
Block Design ◽  
Fruit Trees ◽  
Water Salinity ◽  
Organic Fertilization ◽  
Randomized Block Design ◽  
Irrigation Water Salinity ◽  
Set Up ◽  
Management Alternatives

ABSTRACT The salinity of water and soil reduces the growth and production of crops, especially the fruit trees, such as papaya. Thus, it is necessary to obtain management alternatives for cultivation under these conditions. Therefore, the objective of this study was to evaluate the growth and phytomass of papaya cultivated under irrigation with saline water and organic fertilization. An experiment was set up using a randomized block design, with the treatments distributed in a 5 x 2 factorial scheme, consisting of five levels of salinity of irrigation water (0.6, 1.2, 1.8, 2.4 and 3.0 dS m-1) and two levels of organic fertilization (10 and 20 L of bovine manure per plant), with three replications, totaling thirty experimental plots. Growth variables of papaya were evaluated. Papaya plants were negatively affected by irrigation water salinity, with a greater effect on the number of leaves and on dry phytomass of leaves, with no effect of bovine manure levels.

scholarly journals Initial growth and tolerance of melon cultivars under salt stress

2016 ◽  
Vol 11 (2) ◽  
pp. 462
Author(s):  
Erbia Bressia Gonçalves Araujo ◽  
Francisco Vanies da Silva Sá ◽  
Fernanda Andrade de Oliveira ◽  
Lauter Silva Souto ◽  
Emanoela Pereira de Paiva ◽  
...  
Keyword(s):  
Saline Water ◽  
Block Design ◽  
Salt Content ◽  
Poor Quality ◽  
Tolerance Index ◽  
Initial Growth ◽  
Randomized Block Design ◽  
Initial Stage ◽  
Quality Water ◽  
Poor Quality Water

The melon crop is normally developed in semiarid regions, where water resources are limited. This scarcity of water is a strong stressor on the crops, and requires the supplementation of existing water supplies with poor quality water, especially saline water. This can impede the growth and production of plants; however, the use of tolerant genotypes may minimize this problem. Thus, a greenhouse experiment was developed at the Federal University of Campina Grande - UFCG, Pombal Campus, Paraiba State, Brazil, in order to study the emergence, initial growth, and tolerance of melon cultivars irrigated with waters of different salt content. We studied three melon cultivars (Gaúcho Redondo, Gaúcho Casca de Carvalho and Halles Best Jumbo) irrigated with five levels of saline water (0.6; 1.2; 1.8; 2.4; and 3.0 dS m-1), arranged in a 3 x 5 factorial scheme, with the treatments distributed in a randomized block design with four replications. The plants seeds were monitored for 30 days after sowing, and at 30 days the growth and salinity tolerance index was evaluated. Cultivar Halles Best Jumbo was the most tolerant to saline water during initial stage of growth, while the Gaucho Redondo was more sensitive to salinity. It was found that saline waters up to 1.8 dS m-1 were suitable for irrigation of melon plants round Gaucho and waters up to 2.4 dS m-1 could be used for irrigation of Gaucho Casca de Carvalho and Halles Best Jumbo crops during the initial growth phase.

A comparative study of non-protein nitrogen supplements for sheep

1976 ◽  
Vol 87 (3) ◽  
pp. 567-572 ◽  
Author(s):  
N. W. Offer ◽  
R. A. Evans ◽  
R. F. E. Axford
Keyword(s):  
Ammonium Acetate ◽  
Block Design ◽  
Basal Diet ◽  
Ammonia Nitrogen ◽  
Energetic Efficiency ◽  
Microbial Protein ◽  
Protein Nitrogen ◽  
Randomized Block Design ◽  
Ammonium Lactate ◽  
Gross Energy

SummaryA basal diet, or the basal diet supplemented with equi-nitrogenous amounts of either ammonium lactate, ammonium acetate or urea was fed to four re-entrant cannulated sheep in a randomized block design.Collections of duodenal digesta were carried out automatically for a continuous period of 4 days following a 10-day equilibration period for each sheep on each diet.Supplementation with non-protein nitrogen increased the passage of total amino acids to the duodenum and increased the proportion attributed to microbial protein. It improved pre-duodenal digestibility of modified acid-detergent fibre and increased post-abomasal digestibility of non-ammonia nitrogen, dry matter and gross energy.Supplementation with non-protein nitrogen increased the energetic efficiency of microbial protein production in the rumen.Ammonium lactate and acetate were safe and effective sources of non-protein nitrogen for dietary supplementation.Supplementation with ammonium lactate led to a greater passage of microbial protein to the duodenum than did equi-nitrogenous supplements of urea or ammonium acetate.

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