scholarly journals The Antibacterial Effect of Ethanol Edamame Seeds (Glycine Max (L) Merril) Extract to E.coli Bacteria

2020 ◽  
Vol 6 (3) ◽  
pp. 137
Author(s):  
Diayu Putri Akhita ◽  
Edy Junaidi ◽  
Septa Surya Wahyudi
Keyword(s):  
Average Diameter ◽  
Inhibition Zone ◽  
Negative Control ◽  
The Body ◽  
Control Group ◽  
Control Group Design ◽  
Significant Difference ◽  
Post Test ◽  
The Times

Abstract Infectious diseases can occur in all parts of the body. One of the causes infection in humans is Eschericiae coli bacteria. Eschericiae coli is a rod-shaped bacteria, a gram negative bacteria, facultative aerobics and classified family member of Enterobacteriaceae from the Gammaproteobacteria class. Along the times, E.coli bacteria have resistent to some antibiotics. So we need a new alternative. There is a antibacterial substance in the isoflavon group contained in edamame. Genistein is a main isoflavon in edamame that have antiinflammation, antibacterial, and antioxidant effects. The purpose of this study was to determine is there any antibacterial effects in ethanol edamame seeds extract to E.coli bacteria. This study used a true experimental research design in vitro with a post test only control group design.  The average diameter results of the inhibition zone were analyzed using the Kruskal-Wallis method and obtained p = 0.001 which means there are significant differences in at least two groups. After that, the Mann Whitney post hoc test was conducted and a significant difference was found in the positive and negative control groups for all groups but there was no difference in the treatment group, both groups K1, K2 and K3 for all groups. Keywords : Edamame, Antibacterial, E.coli

scholarly journals The Difference of Antibacterial Power Between Cocoa Peel (Theobroma cacao) Extract 6% Compared to NaOCL 5% Againts Lactobacillus acidophilus

2021 ◽  
Vol 11 (2) ◽  
pp. 67
Author(s):  
Tamara Yuanita ◽  
Dian Agustin Wahjuningrum ◽  
Mega Selvia
Keyword(s):  
Lactobacillus Acidophilus ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Control Group ◽  
Control Group Design ◽  
Post Test ◽  
The Difference ◽  
Peel Extract

Background: Lactobacillus acidophilus is a gram-positive rod-shaped bacteria which mostly causes chronic caries lesions. In the process of cavity preparation it is reported that it is not possible to remove all microorganisms and even bacteria can survive even after removal of the necrotic tissue. Efforts to stop the growth of these bacteria are by chemical cavity cleanser using a 5% NaOCl, but this material have disadvantages including irritating periradicular tissue, unpleasant odor, and toxicity. Due to its disadvantages, it is hoped that natural ingredients can be used as alternatives. Cocoa peel extract has active compounds of tannins, flavonoids, alkaloids, terpenoids, and saponins which have antibacterial ability with a concentration of 6% according to the non-toxic minimum kill concentration of Lactobacillus acidophilus. Purpose: To analyze the difference in antibacterial power between the extract of cocoa bark with a concentration of 6% compared to 5% NaOCl against Lactobacillus acidophilus. Methods: This study was a laboratory experimental in vitro with a post test-only control group design. Using diffusion method against Lactobacillus acidophilus grown in tubes containing BHIB, then cultured in a petridish which contains a nutrient agar and is divided into 2 parts extract cocoa peel extract 6% and NaOCl 5%, then each petridish given a paper disc that has been poured 10μl by each material, then incubated inthe incubator for 2x24 hours at 37oC and observe the diameter of the inhibition zone formed using a caliper. Results: The average diameter of the inhibition zone formed in the 6% cocoa peel extract was 11.8375 mm and NaOCl 5% was 26.0344 mm against Lactobacillus acidophilus. Conclusion: There is a difference in antibacterial power between 6% cocoa peel extract and 5% NaOCl against Lactobacillus acidophilus.

scholarly journals THE EFFECTIVENESS OF SOURSOP LEAF EXTRACT AGAINST GROWTH OF AGGREGATIBACTER ACTINOMYCETEMCOMITANS ATCC® 6514™ IN VITRO

2018 ◽  
Vol 11 (12) ◽  
pp. 411
Author(s):  
Ameta Primasari ◽  
Minasari Nasution ◽  
Nurul Hidayati Arbi ◽  
Dini Permata Sari ◽  
Mohammad Basyuni
Keyword(s):  
Leaf Extract ◽  
Brain Heart Infusion ◽  
Aggregatibacter Actinomycetemcomitans ◽  
Negative Control ◽  
Plate Count ◽  
Control Group ◽  
Control Group Design ◽  
Significant Difference ◽  
Mueller Hinton Agar

Objective: The objective of this study was to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) antibacterial power of soursop leaf extract on Aggregatibacter actinomycetemcomitans (Aa) ATCC® 6514™ growth.Methods: This study was experimental laboratory with post-test only control group design and consists of 8 treatment groups that were soursop leaf extract group with concentration 50%, 25%, 12.5%, 6.25%, 3.125%, and 1.5625% as well as negative control groups were brain heart infusion broth (BHIB) media and chlorhexidine as positive controls. Each treatment was done 3 repetitions. Testing the effectiveness of soursop leaf extract using dilution methods on BHIB and subculture media on Mueller Hinton Agar (MHA) media. The number of Aa ATCC® 6514 ™ colonies was calculated manually using the total plate count method on the MHA media. Data were analyzed using Kruskal–Wallis test (p<0.05) followed by least significance different (LSD) test to see the significant mean difference between treatment groups.Results: Concentration of MIC from soursop leaf extract on Aa ATCC® 6514™ growth was 1.5625% and MBC was 6.25%. LSD assay results showed significant difference effect (p<0.05) Aa ATCC® 6514™ from each treatment group.Conclusion: Soursop leaf extract has antibacterial effectivity against Aa ATCC® 6514 ™.

scholarly journals Porositas Lempeng Resin Akrilik Pasca Perendaman Rebusan Daun Sirih dan Kayu Siwak

2018 ◽  
Vol 4 (1) ◽  
pp. 39
Author(s):  
Yustin Nur Khoiriyah
Keyword(s):  
Acrylic Resin ◽  
Negative Control ◽  
Control Group ◽  
Control Group Design ◽  
Betel Leaf ◽  
Significant Difference ◽  
Antimicrobial Function ◽  
Post Test ◽  
Boiled Water ◽  
One Way Anova

Abstract: Porosity Of Acrylic Resin After Immersion In The Boiled Water Of Betel Leaves And Siwak Wood. Denture acrylic resin can be a collection point for stain, tar, and plaque and this will adversely affect the oral health of the denture wearer. One way to clean the oral cavity of denture users is to use mouthwash and soak the teeth with a cleaning solution/denture cleanser. Mouthwash solutions and chemical-based cleaning solutions at relatively high prices, and affect the porosity of dentures. Therefore, the need for alternative materials that are safe, cheap, natural as well as having antimicrobial function without affecting the level of porosity of denture. This study was to determine the effect of the combination of boiled water of betel leaves and siwak wood to the porosity of the acrylic resin plate. This study was an experimental study, posttest only design with the control group has been done at the integrated laboratory of Poltekkes Tanjungkarang, July – December 2015. Data were analyzed by One Way ANOVA and continued with Least Significant Difference test. The results showed that the combination of betel leaves and siwak wood does not affect the porosity of the acrylic resin plate. The highest concentration of the combination of betel leaf water and siwak wood that did not differ significantly with negative control (aquades) was 75% with longest immersion period was 59 days. Abstrak: Porositas Lempeng Resin Akrilik Pasca Perendaman Rebusan Daun Sirih Dan Kayu Siwak. Gigi tiruan resin akrilik dapat menjadi tempat pengumpulan stain, tar, dan plak dan hal ini akan berpengaruh jelek terhadap kesehatan mulut pemakai gigi tiruan. Salah satu cara menjaga kebersihan rongga mulut pengguna gigi tiruan adalah dengan menggunakan obat kumur dan merendam gigi-tiruan tersebut dengan larutan pembersih/denture cleanser. Larutan obat kumur dan larutan pembersih berbahan dasar dari bahan kimia dengan harga yang relatif mahal, serta mempengaruhi porositas gigi tiruan. Oleh karena itu, perlu adanya bahan alternatif yang aman, murah, alami sekaligus memiliki fungsi antimikroba dengan tanpa mempengaruhi tingkat porositas gigi tiruan. Tujuan penelitian ini adalah untuk mengetahui pengaruh kombinasi air rebusan daun sirih dan kayu siwak terhadap porositas lempeng resin akrilik. Penelitian ini adalah penelitian eksperimental, rancangan post test only with control group design, dilaksanakan di Laboratorium Terpadu Poltekkes Tanjungkarang, Juli-Desember 2015. Analisis data dengan uji One Way ANOVA dan dilanjutkan uji Least Significant Difference. Hasil penelitian menunjukkan bahwa kombinasi air rebusan daun sirih dan kayu siwak tidak mempengaruhi porositas lempeng resin akrilik. Konsentrasi tertinggi dari kombinasi air rebusan daun sirih dan kayu siwak yang tidak berbeda signifikan dengan kontrol negatif (aquades) adalah 75% dengan lama perendaman terpanjang yaitu 59 hari.

scholarly journals Pengaruh Pemberian Ekstrak Rumput Laut Cokelat (Sargassum duplicatum) terhadap Peningkatan Kadar Hematokrit pada Darah Tikus Putih Jantan Galur Wistar (Rattus norvegicus) Anemia yang di Induksi NaNO2

2020 ◽  
Vol 3 (2) ◽  
pp. 38
Author(s):  
Ayu Mayang A. P. ◽  
Liliawanti Liliawanti ◽  
Wachjudi Kurnia
Keyword(s):  
Rattus Norvegicus ◽  
White Male ◽  
Brown Seaweed ◽  
Negative Control ◽  
Laboratory Method ◽  
The Body ◽  
Male Rats ◽  
Control Group ◽  
Control Group Design ◽  
Significant Difference

<p>Anemia is still a high prevalence disease in Indonesia, caused by a lack of iron in the body. The components from brown seaweed such as cobalamin (vitamin B12), pyridoxine (vitamin B6), chlorophyll and iron (Fe) can increase red blood cells in the body. The purpose of this research was to determine the effect of brown seaweed (<em>Sargassum duplicatum</em>) which can increase hematocrit levels in the blood of white male wistar strain rat (<em>Rattus norvegicus</em>) suffered from anemia in induction NaNO<sub>2</sub>.</p><p>            This research uses an experimental laboratory method with a post test only control group design. The samples were 30 male rats divided into 3 treatment groups, the negative control group (K<sub>0</sub>) without treatment, the positive control (K<sub>1</sub>) be treated anemia given NaNO<sub>2</sub> for 18 days without <em>Sargassum duplicatum </em>extract and treatment group (K<sub>2</sub>) treated with anemia given NaNO<sub>2</sub> for 18 days without <em>Sargassum duplicatum </em>extract at a dose of 2,45gr / kgBB. On the 40<sup>th</sup> day, a blood sample was taken, then measured the hematocrit level.</p><p>            The results showed that the mean number of blood hematocrit levels in K<sub>2</sub> was higher than K<sub>1</sub>, it showed a significant difference descriptively. Based on the Kruskal-Wallis test, a value of 0.260 was obtained that p &gt; 0,05 showed that there were no statistically significant differences between groups of experimental animals.</p><p>            From the results of this research, the conclusion is the administration of brown seaweed extract (<em>Sargassum duplicatum</em>) descriptively increased, but not statistically significant.</p><p> </p><p><strong>Keywords :</strong> Anemia, hematocrit, <em>Sargassum duplicatum</em>, NaNO<sub>2</sub></p>

scholarly journals Pengaruh Latihan Renang Teratur dan Latihan Renang Tidak Teratur Terhadap Kadar Trigliserida Plasma pada Rattus norvegicus

2018 ◽  
Vol 2 (1) ◽  
pp. 24-40
Author(s):  
Adi Wijayanto
Keyword(s):  
Rattus Norvegicus ◽  
Exercise Program ◽  
The Body ◽  
Control Group ◽  
Regular Exercise ◽  
Control Group Design ◽  
Group Design ◽  
Significant Difference ◽  
Post Test ◽  
And Control

Triglycerides are source of energy in the body, but triglyceride deposits that exceed the threshold will cause health problems, so triglycerides should be lowered to normal. The reduction of triglycerides can be done through physical activity, therefore this study aims to reveal the effect of regular swimming exercises and irregular exercises against triglyceride levels. This research is an experimental research using The Post Test Only Control Group Design design. Subjects of the study were male of Rattus Norvegicus Wistar, 39 subjects was divided into 3 groups with Matched Subject Design technique, which consisted of 2 groups of treatment that is regular and irregular group, and control group. The exercise program was given a swimming with a 3% weight of body weight and was tied 5 cm from the tip of the tail which was performed 3 times per week consistently for regular exercise and 0 to 5 times per week inconsistently for irregular exercise for 12 weeks, While the control group was not given training. In the last week before the blood was taken, the first three groups were given swimming treatment exercises. Based on the results of the anava test, there was a significant difference in the reduction of triglyceride levels among white mice who were given regular exercise with irregular exercise. These results suggest that regular exercise has a better impact on triglyceride reduction than irregular exercise, due to the higher adaptation process of regular exercise.

scholarly journals Efektivitas perasan daun seledri (Apium graveolens Linn.) sebagai pembersih gigi tiruan terhadap pertumbuhan Candida albicans pada basis gigi tiruan nilon termoplastikThe effectiveness of celery leaf juice (Apium graveolens Linn.) as a denture cleaner against the growth of Candida albicans on thermoplastic nylon denture base

2020 ◽  
Vol 32 (3) ◽  
pp. 184
Author(s):  
Dhilan Purna Aji ◽  
Achmad Gunadi ◽  
Tantin Ermawati
Keyword(s):  
Candida Albicans ◽  
Positive Control ◽  
Negative Control ◽  
Apium Graveolens ◽  
Control Group ◽  
Control Group Design ◽  
Denture Base ◽  
Significant Difference ◽  
Post Test ◽  
Leaf Juice

Pendahuluan: Basis gigi tiruan nilon termoplastik merupakan alternatif pengganti basis gigi tiruan resin akrilik. Pembersihan gigi tiruan diperlukan untuk menjaga basis gigi tiruan agar tetap bersih dari kontaminasi mikroorganisme. Daun seledri merupakan bahan alam yang memiliki kandungan flavonoid, saponin, tanin, dan minyak atsiri yang sudah terbukti bersifat antijamur. Penelitian sebelumnya hanya melihat zona hambat saja. Tujuan penelitian adalah menganalisis efektivitas perasan daun seledri sebagai pembersih gigi tiruan terhadap pertumbuhan Candida albicans pada basis gigi tiruan nilon termoplastik. Metode: Eksperimental laboratoris dengan rancangan penelitian post-test only control group design. Sampel nilon termoplastik berbentuk cakram dengan diameter 10 mm dan tebal 2 mm  sebanyak 25 dibagi menjadi 5 kelompok. Sampel dikontaminasi dengan C. albicans setelah direndam dalam kontrol negatif (akuades) selama 6 jam, kontrol positif (tablet effervescent) selama 15 menit, dan perasan daun seledri konsentrasi 25, 50, dan 100% selama 6 jam. Sampel selanjutnya diletakkan dalam media sabouraud dextrose broth (SDB). Pengujian efektivitas perasan daun seledri dilakukan dengan menentukan nilai absorbansi kekeruhan media SDB menggunakan spektrofotometer. Data dianalisis menggunakan one-way ANOVA dan Least Significant Difference (LSD). Hasil: Nilai konsentrasi C. albicans pada kelompok kontrol negatif mendapatkan hasil 0,380 x 108 CFU/mL, kontrol positif mendapatkan hasil 0,310 x 108 CFU/mL, perasan daun seledri konsentrasi 25% mendapatkan hasil 0,243 x 108 CFU/mL, konsentrasi 50% mendapatkan hasil 0,160 x 108 CFU/mL, dan konsentrasi 100% mendapatkan hasil 0,236 x 108 CFU/mL. Simpulan: Perasan daun seledri efektif sebagai pembersih gigi tiruan untuk menghambat  pertumbuhan C. albicans, dengan konsentrasi yang efektif sebesar 50%.Kata kunci: Nilon termoplastik, perasan daun seledri, Candida albicans. ABSTRACTIntroduction: Thermoplastic nylon denture base is an alternative to the acrylic resin denture base. Denture cleaning is needed to keep the denture base clean from contamination by microorganisms. Celery leaves are natural ingredients that contain flavonoids, saponins, tannins, and essential oils that have been proven to have antifungal properties. Previous research only observed the inhibitory zone. This research was aimed to analyse the effectiveness of celery leaf juice as a denture cleaner against the growth of Candida albicans on thermoplastic nylon denture base. Methods: Experimental laboratory with a post-test only control group design was conducted towards 25 disc-shaped thermoplastic nylon samples with a diameter of 10 mm and a thickness of 2 mm, and divided into five groups. Samples were contaminated with C. albicans after immersion in the negative control (aquadest) for 6 hours, positive control (effervescent tablets) for 15 minutes, and celery leaf juice with the concentrations of 25, 50, and 100% for 6 hours. The sample was then placed in the Sabouraud-dextrose broth (SDB) medium. The effectiveness of celery leaf juice was tested by determining the absorbance value of SDB media turbidity using a spectrophotometer. Data were analysed using one-way ANOVA and Least Significant Difference (LSD). Results: The concentration value of C. albicans in the negative control group was 0.380 x 108 CFU / mL, the positive control was 0.310 x 108 CFU / mL, the juice of celery leaves with the concentration of 25% was 0.243 x 108 CFU / mL, the concentration of 50% was 0.160 x 108 CFU / mL, and the concentration of 100% was 0.236 x 108 CFU / mL. Conclusion: Celery leaf juice is effective as a denture cleaner to inhibit the growth of C. albicans, with an effective concentration of 50%.Keywords: Thermoplastic nylon, celery leaf juice, Candida albicans.

scholarly journals Pengaruh Pemberian Glutamin pada Kemampuan Fagositosis Makrofag terhadap Pseudomonas Aeruginosa

2015 ◽  
Vol 4 (3) ◽  
Author(s):  
Daslina Daslina ◽  
Eryati Darwin ◽  
Aziz Djamal
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Immune System ◽  
Amino Acid ◽  
T Test ◽  
The Body ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Significant Difference ◽  
Post Test

Abstrak Pseudomonas aeruginosa adalah bakteri penyebab infeksi terbanyak yang resisten terhadap antibiotik. Glutamin adalah asam amino yang terdapat dalam tubuh yang salah satu fungsinya dapat memodulasi imunitas tubuh. Tujuan penelitian ini adalah menentukan potensi glutamin dalam meningkatkan kemampuan sistem imun terhadap infeksi P. aeruginosa. Penelitian eksperimental dengan post test only control group design telah dilakukan terhadap 24 ekor mencit usia 6-8 minggu dengan berat 30 gr. Mencit dibagi ke dalam dua kelompok, yaitu kontrol (K) danperlakuan (P) yang diberi glutamin dengan dosis 30 mg/kg/hari selama 14 hari. Isolasi makrofag peritoneum mencit dilakukan pada hari ke-15 dan dilakukan uji fagositosis menggunakan latex dan bakteri P. aeruginosa. Pengamatan dilakukan terhadap persentase makrofag aktif terhadap latex dan P. aeruginosa. Hasil pengamatan dan analisisstatistik menggunakan metode t-test menunjukkan adanya perbedaan yang signifikan antara kontrol dan perlakuan (p<0.05). Persentase makrofag aktif terhadap latex adalah 0,63 ± 0,058 (K) dan 0,84 ± 0,04 (P), sedangkan terhadap P. aeruginosa adalah 0,56± 0,07 (K) dan 0,80± 0,03 (P). Terlihat bahwa angka persentase fagositosis terhadap P.aeruginosa lebih kecil karena adanya kemampuan bakteri untuk menghadapi makrofag dibandingkan latex. Kesimpulan yang diperoleh dari penelitian ini adalah bahwa asam amino non-esensial glutamin memiliki pengaruh untuk meningkatkan kemampuan sistem imun tubuh. Kata kunci: glutamin, fagositosis makrofag, Pseudomonas aeruginosa Abstract Pseudomonas aeruginosa is the most common cause of bacterial infections that are resistant to antibiotics. Glutamine is an amino acid  in the body that able to modulate the body's immune function. The objective of this study was to determine the potential of glutamine in enhancing the ability of the immune system against infection of P.aeruginosa. Experimental research with post test only control group design was conducted on 24 male minutes 6-8 weeks of age weighing 30 grams. Mice were divided into two groups: control (K) and treatment (P) are given glutamine at a dose of 30 mg / kg / day for 14 days. Isolation of peritoneal macrophages of mice performed on day-15 and testedusing latex and bacterial phagocytosis of P. aeruginosa. Observations were made of the percentage of activated macrophages toward latex and P. aeruginosa. The observation result and statistical analysis using t -test showed a significant difference between the control and treatment (p <0.05). The percentage of active macrophages to latex was0.63 ± 0.058 (K) and 0.84 ± 0.04 (P), while to P. aeruginosa is 0.56 ± 0.07 (K) and 0.80 ± 0.03 (P). That seein it percentage of phagocytosis against P. aeruginosa smaller than compared to latex. Because of the ability of bacteria to confront macrophages. The conclusion from this study is a non-essensial amino acid glutamine has the effect to increase the ability of the body's immune system. Keywords: glutamin, immunity phagocytosis macrophage, Pseudomonas aeruginosa 

scholarly journals Effect of putri malu (Mimosa pudica) extract on Ascaris suum mortality in vitro

2011 ◽  
Vol 9 (2) ◽  
pp. 33-37
Author(s):  
MUHAMMAD ARIF NUR SYAHID ◽  
CR. SITI UTARI ◽  
SUTARMIADJI DJUMARGA
Keyword(s):  
Ascaris Suum ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Mimosa Pudica ◽  
Control Group Design ◽  
Significance Level ◽  
Death Time ◽  
Significant Difference

Syahid MAN, Utari CRS, Djumarga S. 2011. Effect of putri malu extract (Mimosa pudica) on Ascaris suum mortality in vitro. Biofarmasi 9: 33-37. This study was to determine the influence of Mimosa pudica extract in Ascaris suum mortality. This research was a laboratory experiment, with a post-test only with control group design by using 140 adult A. suum, divided into seven groups. This research used NaCl 0.9% for a negative control, pirantel pamoat 5 mg/mL solution for a positive control, and five intervention by using 20%, 40%, 60%, 80% and 100% concentration of M. pudica extract. The observation was conducted in every two hours until worm death and it was started to be counted after all worm death. Data were analyzed with one-way ANOVA test continued with Least Significance Difference (LSD) by using SPP for Window Release 17 with a significance level p<0.05. The results showed that all A. suum death in 96 hours at negative control, 2 hours at positive control, 29.5 hours at 20% M. pudica extract, 24.5 hours at 40% M. pudica extract, 16 hours at 60% M. pudica extract, 12 hours at 80% M. pudica extract and 4 hours at 100% M. pudica extract. There was a significant difference in the death time of A. suum in all research groups. From the result of research, it could be concluded that the extract of putri malu had an effect on accelerating A. suum mortality time.

scholarly journals POTENTIAL ETHANOL OF Carica papaya L. EXTRACT AS IMMUNOMODULATORY THROUGH HISTOLOGY OBSERVATION AT MICE BALB/C SPLEEN

2017 ◽  
Vol 13 (2) ◽  
pp. 205
Author(s):  
Sri Tasminatun ◽  
Ria Pravitasari ◽  
SN. Nurul Makiyah
Keyword(s):  
Treatment Group ◽  
Carica Papaya ◽  
Negative Control Group ◽  
Negative Control ◽  
The Body ◽  
Control Group ◽  
Control Group Design ◽  
Post Test ◽  
One Way Anova

Abstract: The diameter of the alba pulp of the spleen will be enlarged, when the body responds to allergens by increasing the activity of the immune system. Papaya fruits (Carica papaya L.) containing flavonoid that can be as an immunomodulator. This study aims to determine the effect of C. papaya extract to the diameter size alba pulp of the spleen of Ovalbumin (OVA) induced Balb/C mice. The research design was experimental in vivo animal test with post-test only control group design. Thirty male Balb/C mice, ±20grams weight, 8 weeks old, divided into six groups consists of a normal control, negative control, Methylprednisolone, and C. papaya extract treatment group (175mg/kg, 350mg/kg and 700mg/kg dose per day). The extract and Methylprednisolone are given for 28 days, while OVA is given on 15thand 22ndday (intraperitoneal,) and 23rd until 28thday (peroral). On the 29thday the mice are sacrificed and the duodenum was made into histological preparation with HE staining. The immunomodulator effect is assessed by the average of the diameter size alba pulp of the spleen under a light microscope. The data were analyzed using SPSS15 with one way ANOVA continued with Tukey test. The average of the diameter size alba pulp of the spleen for control group is 10,165 ± 0,195; negative control group is 30,357 ± 0,826; methylprednisolone group is 10,220 ± 0,346; 175mg/kgbb dose extract is 14,956 ± 1,057; 350mg/kgbb dose extract is 14,030 ± 0,667; and 700mg/kgbb dose extract is 12,504 ± 0,729. The conclusion of this study is Carica papaya L. extract treatment group (175mg/kg, 350mg/kg and 700mg/kg dose per day) has an effect to the average of the diameter size alba pulp of the spleen in OVA-induced Balb/C mice significantly. 700mg/kg dose per day has the same effect with the methylprednisolone group. Keywords: immunomodulator, Carica papaya L., alba pulp of the spleen, ovalbumin

scholarly journals PERBEDAAN ZONA HAMBAT PERTUMBUHAN Staphylococcus aureus PADA BERBAGAI KONSENTRASI REBUSAN DAUN SALAM (Syzygium polyanthum) SECARA IN VITRO

2018 ◽  
Vol 5 (2) ◽  
Author(s):  
Nyoman Mastra
Keyword(s):  
Staphylococcus Aureus ◽  
Growth Inhibition ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Negative Control ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Growth Inhibition Zone ◽  
Significant Difference

AbstractBackground Staphylococcus aureus is one of causes infection and this bacteria have been resistence for many antibiotic. Bay leaf have antibacterials substance, which stew leaves can be treat infection caused Staphylococcus aureus. The purpose of this study was to determine differences in growth inhibition zone of Staphylococcus aureus at various concentrations of water stew of bay leaf.Method The method of this study is true experiment with posstest only control design, and used Kirby Bauer disk diffusion method with various concentrations of water stewed of bay leaf (20%, 40%, 60%, 80%,100%), positive control (chloramfenicol 30 μg) and negative control (sterile distilled water). Result The result showed that the average diameter of inhibition zone in concentration 20%, 40%, 60%, 80%, and 100% is 7 mm, 8,4 mm, 9,6 mm, 10,5 mm and 11,5 mm. Based on statistical analysis using oneway ANOVA available the value of p (0,000) α (0,05), so the inhibition zone is significant difference of growth inhibition zone of Staphylococcus aureus at various concentrations of stewed water bay leaf. Conclusion Water stew of bay leaf can inhibit the growth of Staphylococcus aureus, and there are differences in growth inhibition zone of Staphylococcus aureus at various concentrations of water stew of bay leaf. Keywords: stew of bay leaf; Staphylococcus aureus; inhibition zone 

Sign in / Sign up

Export Citation Format

Share Document