scholarly journals PENGARUH PEMBERIAN EKSTRAK SAMBILOTO (Andrographis Paniculata) TERHADAP GAMBARAN HISTOPATOLOGI GINJAL YANG DIINDUKSI GENTAMISIN PADA TIKUS (Rattus norvegicus)

2020 ◽  
Vol 8 (1) ◽  
pp. 29
Author(s):  
Anggi Dwi Nugrahani ◽  
Lianny Nangoi ◽  
Dewa Ketut Meles ◽  
Thomas Valentinus Widiyatno ◽  
Iwan Sahrial Hamid ◽  
...  
Keyword(s):  
Statistical Analysis ◽  
Rattus Norvegicus ◽  
Intramuscular Injection ◽  
Treated Group ◽  
Negative Control ◽  
White Rats ◽  
Renal Histopathology ◽  
Best Value ◽  
Significant Difference ◽  
Carboxy Methyl

This research was aimed to know the effect of leaves extract of sambiloto as a nephroprotector on renal histopathology features induced by gentamicin in white rats (Rattus norvegicus). The effect of leaves extract of sambiloto was tested in 25 rats. Twenty five male white rat were randomly divided into five groups; K- as negative control was given carboxy methyl cellulosa in 15 days, P0 was given carboxyl methyl cellulosa in 15 days, P1 was given leaves extract of sambiloto of 177 mg/kg bw/day/po in 15 days, P2 was given leaves extract of sambiloto of 189 mg/kg bw/day/po in 15 days, P3 was given leaves extract of sambiloto of 239,5 mg/kg bw/day/po in 15 days. On the 15 day of experimental, P0, P1, P2, P3 was given 200 mg/kg bw of gentamicin. Gentamicin solutions on P0, P1, P2 and P3 treated through intramuscular injection. After 24 hours of gentamicin induced, take the rats renal by laparotomy and then making preparations histopathology with staining Haematoxylin-Eosin (HE). Based on the result of Kruskall-Wallis statistical analysis on renal histopathology showed this research that the treatment have significant difference (p < 0,05). The leaves extract of sambiloto treated group (P3) gave the best value on the renal morfology, it gave a good effects in preventing nephrotoxicity in white rats.

scholarly journals The Effect of Ethanol Extract of Malacca Leaves (Phyllanthus emblica) on The Number of Fibroblast Cells in White Rats (Rattus norvegicus) Burns Wound

2020 ◽  
Vol 5 (2) ◽  
pp. 7-12
Author(s):  
Ajirni Ajirni ◽  
Nazaruddin Nazaruddin ◽  
Amalia Sutriana ◽  
Dian Masyitha ◽  
Muhammad Isa
Keyword(s):  
Rattus Norvegicus ◽  
Heat Exposure ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Fibroblast Cells ◽  
Phyllanthus Emblica ◽  
White Rats ◽  
Degree Burn ◽  
Significant Difference

Burns are caused by heat exposure, such as fire, radiation, electricity or chemicals that can damage the skin and affect the body's systems. The aim of this study was to find out the effect of the ethanol extract of Malacca leaves (Phyllanthus emblica) on the number of fibroblast cells in white rats (Rattus norvegicus) that have burned. This study used 24 white rats (Rattus norvegicus) were divided into 4 groups that smeared with aquadest as a negative control (P1), 5% ethanol extract gel of Malacca leaves (P2), 10% ethanol extract gel of Malacca leaves (P3), and positive control applied with bioplasenton® gel (P4). The IIA degree burn were created by placing a 2x2 cm hot iron plate on the back of the rat for 5 seconds. The euthanasia performed to all animal and the skin samples were collected after 15 days of treatment. Then histopathological preparations were made using HE staining. The number of fibroblast cells were analyzed by ANOVA test. The average number of white rats (Rattus norvegicus) fibroblast cells that suffered burns P1 (negative control) had a number of 7 ± 1.4 cells/visual. Whereas th e P2 group had a number of 4.2 ± 1.58 cells/visual. This value has a significant difference with the negative control. But the P2 and P3 values (3 ± 1.51 cells/visual) there is no significant different with the P4 value (positive control) with an average number of P4 fibroblast cells were 2 ± 0.4 cells/visual. The results of this study concluded that the ethanol extract of malacca leaves 5% and 10% had an effect againts accelerating burns healing in white rats ( Rattus norvegicus).

scholarly journals The Influence of Green Tea Infusion (Camellia sinensis) on Renal Histopathological Overview of Male White Rats (Rattus Norvegicus) Sprague Dawley Strain Induced by Ethanol

2020 ◽  
Vol 10 (3-s) ◽  
pp. 111-115
Author(s):  
Muhartono Muhartono ◽  
Katya Rizqita Fitriana
Keyword(s):  
Green Tea ◽  
Camellia Sinensis ◽  
Rattus Norvegicus ◽  
Positive Control ◽  
Negative Control ◽  
Sprague Dawley ◽  
Tea Infusion ◽  
White Rats ◽  
Renal Histopathology ◽  
Keywords Alcohol

Aims: This sudy aims to determine the effect infusion  of green tea (Camellia sinensis) to the renal histopathology picture of male white rats (Rattus norvegicus) Sprague dawley strain ethanol-induced. Methods: This study used 30 rats divided into 5 groups. The negative control (K-) was given distilled water 2 mL/day, the positive control (K +) was given ethanol 2 mL/day, the treatment group (P1, P2, P3) was given ethanol 2 mL and green tea infusion with respective doses of 0.375, 0.75 and 1.5 grams orally for 14 days. Then the renal retrieval is done to be examined microscopically. Results: The mean kidney damage score obtained was K- = 0.36; K + = 3.24; P1 = 2.52; P2 = 2,4; and P3 = 2.2. Data analysis with One Way Anova Test and continued with Pos Hoc and found significant differences in K- to K+, P1, P2, P3; K+ is significant to K-, P1, P2, P3; and P1, P2, P3 are significant to K- & K+. Conclusion: There is an effect of infusion of green tea (Camellia sinensis) on the renal histopathological picture of male white rats (Rattus norvegicus) ethanol-induced Sprague dawley strain. Keywords: alcohol, antioxidant, ethanol, green tea, renal histopathology

scholarly journals The Effect of Red Pitaya Peel (Hylocereus polyrhizus Extract) on Malondialdehida Levels and Histopathology Profile in Diazinon Induced Rat (Rattus norvegicus)

2019 ◽  
Vol 10 (2) ◽  
pp. 88
Author(s):  
Chanif Mahdi ◽  
Viski Fitri Hendrawan ◽  
Khoirus Viestaria
Keyword(s):  
Rattus Norvegicus ◽  
Negative Control ◽  
The Body ◽  
Control Treatment ◽  
Average Weight ◽  
Organophosphate Insecticide ◽  
White Rats ◽  
Significant Difference ◽  
Peel Extract ◽  
Hylocereus Polyrhizus

Diazinon, an organophosphate insecticide is widely used in agricultural sectors. The metabolic products of this organophosphate compound can increase Reactive Oxygen Species (ROS) in the body. It causes complications to the various organs, one of which is gastric. Red pitaya peel extract (Hylocereus polyrhizus) has high antioxidant activity in lowering ROS, in cases of diazinon poisoning. This study aims to determine the effectiveness of redpitaya peel extract in reducing the levels of Malondialdehyde (MDA) and repairing histopathological damage of rats induced by diazinon. This study used 20 tails of male white Wistar rats (Rattus norvegicus) from 8-12 weeks of age with the average weight of 150 grams . The subject was divided into 5 groups, which were negative control (-), positive control (+), treatment 1 (P1), treatment 2 (P2), and treatment 3 (P3). A dose of 40 mg/kg BW of Diazinon was given orally every day through feeding tube (sonde) for 5 consecutive days. Red pitaya peel extract was given to test groups with a dose of 150 mg/150 g BW, 200 mg/150 g BW and 250 mg/150 g BW, for 14 days. MDA levels were measured using the Thiobarbituric acid (TBA) test. Gastric histopathology features were stained with Hematoxylin-Eosin (HE) after 14 days. The MDA levels were analyzed quantitatively by ANOVA using SPSS version 22 for Windows and continued with honestly significant difference (HSD) test (α = 5%) and gastric histopathology were analyzed descriptively. The results showed that the extract of red pitaya peel with dose of 150 mg/150 g BW significantly (p<0.01) reduces MDA level in gastric dan improves the histopathology of the gastric. Red pitaya peel extract at a dose of 150 mg/150 g BW was able to significantly decrease MDA levels and improve the histopathology feature of the gastric in white rats induced by diazinon. So this can be summarised that giving red pitaya extract in rats have a very significant effect on the level of malondialdehyde production and it could repair rats stomach tissue that induced by diazinon.Keywords: diazinon, histopathology, MDA, red pitaya peel

scholarly journals PENGARUH GELATIN IKAN PATIN (PANGASIUS DJAMBAL) TERHADAP JUMLAH FIBROBLAS PADA LUKA PASCA PENCABUTAN GIGI TIKUS PUTIH (RATTUS NORVEGICUS)

2019 ◽  
Vol 6 (1) ◽  
pp. 1
Author(s):  
Fredy Mardiyantoro ◽  
Fidya Fidya ◽  
Dena Savira Andriani
Keyword(s):  
Rattus Norvegicus ◽  
Tooth Extraction ◽  
Control Group ◽  
Fish Gelatin ◽  
Tukey Test ◽  
White Rats ◽  
Significant Difference ◽  
Post Hoc ◽  
Hematoxylin Eosin ◽  
Test Result

Background: Gelatin has been used as a hemostatic agent to stop bleeding and it can be absorbed by human body within 6 hours. The patin fish (Pangasius djambal) gelatin has a high amount of amino acid, especially glutamine which has an important role in increasing the proliferation of fibroblast in wounds. The purpose of this study was to determine the effect of patin fish gelatin to the number of fibroblast in wound after tooth extraction.Method: Rat’s tooth extraction is done on the lower left incisive tooth. Thirty white rats were divided into two groups randomly with 3 time series. Control group was the rats without given of patin fish gelatin and treatment group was the rats given 1cc of patin fish gelatin with pipette. Socket taken for histologically processed by Hematoxylin Eosin staining.Result: It showed there were significant differences in the number of fibroblasts between groups. The Post Hoc Tukey test result showed there was a significant difference between the control group and the treatment group.Conclusion: Patin fish gelatin has effect increasing the number of fibroblast inwound after white rat’s (Rattus norvegicus) tooth extraction

scholarly journals HEPATOPROTECTIVE ACTIVITY OF VERNONIA AMYGDALINA LEAF ETHANOLIC EXTRACT IN WHITE RATS INDUCED BY PARACETAMOL

2018 ◽  
Vol 11 (10) ◽  
pp. 562
Author(s):  
Panal Sitorus ◽  
Nerdy Nerdy
Keyword(s):  
Body Weight ◽  
Methyl Cellulose ◽  
Ethanolic Extract ◽  
Scientific Data ◽  
Control Group ◽  
Carboxy Methyl Cellulose ◽  
Vernonia Amygdalina ◽  
White Rats ◽  
Significant Difference ◽  
Carboxy Methyl

Objectives: A study on the hepatoprotective effect of Vernonia amygdalina leaves ethanolic extract on white rats induced by paracetamol was aimed to obtaining scientific data and evidence the hepatoprotective ability of the extract.Methods: The research was a complete randomized design, using 36 white rats which were divided into 6 groups. The normal control group was given 1% carboxy methyl cellulose for 7 days and followed by distilled water 8 hours after treatment. The positive control group was given 100 mg curcumin per kg body weight of rats for 7 days and followed by 2.5 g paracetamol per kg body weight after treatment. The negative control group was given 1% carboxy methyl cellulose for 7 days and followed by 2.5 g paracetamol per kg body weight after treatment. The test group was given 50, 100 and 200 mg extract per kg body weight of rats for 7 days and followed by 2.5 g paracetamol per kg body weight after treatment. Blood sampling was carried out 3 times (before treatment, after treatment and after induction).Results: With doses of 50, 100, and 200 mg extract per kg body weight of rats give significant difference on the decrease of Serum Glutamic Oxaloacetic Transaminase (SGOT) and Serum Glutamic Pyruvic Transaminase (SGPT) levels after paracetamol administration.Conclusions: It can be concluded that Vernonia amygdalina leaves ethanolic extract were potentially hepatoprotective.

scholarly journals HISTOPATHOLOGICAL STRUCTURE OF WHITE RATS LIVER AFTER GIVING ANT NEST EXTRACT DUE TO INDUCED PARACETAMOL TOXIC DOSE

2019 ◽  
pp. 14
Author(s):  
I Made Merdana ◽  
I Made Kardena ◽  
Ketut Budiasa ◽  
I Made Dodi Gunawan
Keyword(s):  
Rat Liver ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Toxic Dose ◽  
White Rats ◽  
Significant Difference ◽  
Ant Nest ◽  
Histopathologic Changes

This study aim was to determine the influence ant nest plant extract (Myrmecodia pendans) on histopathological changeof white rat liver (Rattus novergicus) due to induced with paracetamol toxic dose. This study used 24 male white rats, divided into four groups, negative control group (P0) given placebo, positive control group (P1) given paracetamol dose 250 mg / kg bw for 10 days, P2 given ant nest extract 250 mg / kg bw and paracetamol dose 250 mg / kg bw for 10 days, P3 given ants nest extract 250 mg / kg bw for seven days, then continued by giving paracetamol and ants nest extract with dose 250 mg / kg bw for ten days. After the treatment done, all the rats were dinecropsed. Liver organs were taken and processed for making histopathology preparations. Parameters examined included hemorrhage, congestion, degeneration and necrosis. The data obtained were analyzed statistically by using Kruskal Wallis test followed by Mann Whitney test. Mann Whitney test results for all categories of histopathologic changes in  hemorrhagic, congestion, degeneration, and necrosis between negative control group (P0) and positive control group (P1) were significantly different (P <0.05), between negative control (P0) with P2 and P3 there was no significant difference (P> 0,05). Afterward, between the positive control (P1) and P2 with P3 there was a significant difference (P <0.05). I can be concludedthat the administration of paracetamol dose 250 mg/kg bw for 10 days affects the histopathologic changes of white rat liver. The administration of ant nest plant extracts can reduce the side effects of toxic doses of paracetamol.

scholarly journals MAT, a Novel Polyherbal Aphrodisiac Formulation, Enhances Sexual Function and Nrf2/HO-1 Pathway While Reducing Oxidative Damage in Male Rats

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Kazim Sahin ◽  
Mehmet Tuzcu ◽  
Cemal Orhan ◽  
Hasan Gencoglu ◽  
Nurhan Sahin ◽  
...  
Keyword(s):  
Sexual Function ◽  
Treated Group ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Mucuna Pruriens ◽  
Tribulus Terrestris ◽  
Sexual Hormones ◽  
Significant Difference ◽  
Group 5

Mucuna pruriens, Ashwagandha, and Tribulus terrestris are known as the enhancers for sexual health, functional activities, vitality, and longevity. These herbs had been widely used in the Ayurveda medicine as aphrodisiacs through the ages, and their efficacy was also verified separately in our previous publication. Therefore, the aim of this study was to determine the effects of Mucuna, Ashwagandha, and Tribulus complexes on sexual function in rats. Twenty-eight male rats allocated to four groups as follows: (i) negative control (C); (ii) positive control or sildenafil citrate treated group (5 mg/kg) (S); (iii) MAT1 (combination of 10 mg Mucuna (M) + 10 mg Ashwagandha (A) + 10 mg Tribulus (T)/kg BW); (iv) MAT 2 (20 mg Mucuna + 20 mg Ashwagandha + 20 mg Tribulus/kg BW). There was no significant difference found between the MAT1 and MAT2 groups while they showed significantly increased testosterone, follicle-stimulating hormone (FSH), and luteinizing hormone (LH) levels when compared to the negative control. Significant increases in Nrf2/HO1 levels and decreases in NF-κB were detected in MAT groups similar to the decrease in serum and testis malondialdehyde (MDA) levels as compared to both controls. The sperm motility, count, and rate also significantly improved in both MAT groups, while ALT, AST, creatinine, ALP, and urea levels did not change in any of the groups. Oral consumption of MATs combination in male rats resulted in inhibition of NF-κB and MDA and also increased sex hormones with Nrf2-mediated HO-1 induction. MAT combinations may improve sexual functions by increasing levels of sexual hormones and regulation of NF-κB and Nrf2/HO-1 signaling pathways.

scholarly journals Pengaruh Kitosan Terhadap Jumlah Osteoklas dan Osteoblas Pada Tikus Galur Wistar Model Menopause

2017 ◽  
Vol 1 (2) ◽  
pp. 76-87
Author(s):  
Muhammad Nur
Keyword(s):  
Treatment Group ◽  
Total Sample ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Control Group Design ◽  
White Rats ◽  
Significant Difference

Chitosan is one of the most abundant and dispersed polymers in nature. It has a rapid healing effect for tissues and has an effect on bone remodeling. The purpose of this study was to prove that giving chitosan influenced the decrease of osteoclast number and increasing number of osteoblasts in female mice of wistar strain of ovariectomy menopause model.This study used a posttest only control group design. The total sample of 30 rats divided into three groups was randomized, osteoporosis was made with the menopausal model which was performed ovariectomy in the positive control group and the treatment group then in the treatment group was given chitosan extract of single dose of 45 mg / day via Nasogastric Tube (NGT). K1 was a negative control group (without ovariectomy and without chitosan), K2 was a positive control group (with no chitosan ovariectomy), K3 was the treatment group (ovariectomy and chitosan 45 mg / day).The results showed significant differences in the number of osteoblasts between K1 and K3, K2 with K3, and no significant differences were found in the K1 group with K2. While the number of osteoclasts showed that there were significant differences in K1 group with K2 and no significant difference was found in K1 group with K3, K2 with K3.Conclusions in this study showed that 45 mg / day of chitosan doses could significantly increase osteoblast counts in menopause female white rats and decrease the number of osteoclasts performed by ovariectomy with chitosan administration.

scholarly journals Effect of Giving Mahkota Dewa Fruits (Phaleria Macrocarpa) Extract to Epithelialization In Incision Wound of White Rats (Rattus Norvegicus)

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Idola Perdana Sulistyoning Suharto
Keyword(s):  
Treatment Group ◽  
Rattus Norvegicus ◽  
Male Rats ◽  
Control Group ◽  
P Value ◽  
Control Group Design ◽  
White Rats ◽  
Significant Difference ◽  
Phaleria Macrocarpa ◽  
Incision Wound

The research purpose was to analysis effect of giving mahkota dewa fruits (Phaleria macrocarpa) extract to epithelialization in incision wound of white rats (Rattus norvegicus). The method was randomized posted-only control group design. There were 30 male rats (Rattus norvegicus) grouped on control and treatment group. Control group divided into three groups (KK1, KK2, KK3) and also treatment group divided into three groups (KP1, KP2, KP3). Control group just given CMC 1% peroral without mahkota dewa fruits extract, the treatment group given mahkota dewa fruits extract 22.5 mg/kg body weight. The data was analyzed by Kruskall Wallis. Based on Kruskall Wallis test, obtained result that there was a significant difference (p<0.05) epithelialization variable with p value p = 0.000 between control and treatment group. And based on One-way Anova test, obtained result that there was a significant difference (p<0.05) with p value p =0.000 between control and treatment group. The conclusion of this research was giving mahkota dewa fruits (Phaleria macrocarpa) extract can increase epithelialization in incision wound of white rats (Rattus norvegicus). Keywords : Mahkota Dewa Fruits (Phaleria Macrocarpa) Extract, Epithelialization, Incision Wound 

scholarly journals Skrining Fitokimia dan Potensi Antidiabetes Ekstrak Etanol Herba Ciplukan (Physalis Angulata L) pada Tikus Putih (Rattus Novergicus) yang Diinduksi Aloksan

2019 ◽  
Vol 4 (3) ◽  
pp. 98-107
Author(s):  
Hendra Pratama Maliangkay ◽  
Rolef Rumondor ◽  
Mynia Kantohe
Keyword(s):  
Blood Glucose ◽  
Rattus Norvegicus ◽  
Ethanol Extract ◽  
Negative Control ◽  
Glucose Measurement ◽  
Percentage Decrease ◽  
Glucose Levels ◽  
White Rats ◽  
Blood Glucose Levels ◽  
Physalis Angulata

The purpose of this study to determine the anti-diabetic potential of ciplukan herbal ethanol extract (Physalis angulata L) in white rats (Rattus norvegicus) induced alloxan given intraperitoneally and comparing its effectiveness with glibenclamide. This study uses 5 groups namely giving aquadest as normal control, giving alloxan as a negative control, giving alloxan and glibenclamide as a positive control and the ethanol extract of ciplukan herbal with a dose of 150 mg/kg BB and 300 mg/kg BB. Blood glucose measurement performed on day 0 (three days after induction), day 7, and day 14. The results showed that ethanol extract of ciplukan herbal (Physalis angulata L) can lower blood glucose levekls. A dose of 150 mg/kg BB have percentage decrease in blood glucose levels on day 7 and day 14 respectively by 14.1%and 70.5%. dose of 300 mg/kg BB have a percentage decrease in blood glucose levels on day 7 and day 14 respectively by 70.5%.and 69.5%. a dose of 150 mg/kg BB is more effective as an anti-diabetic compared with a dose of 300 mg/kg BB. Ethanol extract of ciplukan herbal (Physalis angulata L) also has an activity to repair damaged pancreatic-β cells in rats (Rattus norvegicus) induced by alloxan.

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