scholarly journals Analisis Respons Imun Ikan Koi (Cyprinus carpio Koi) yang Divaksin dengan Whole Protein Spora Myxobolus koi sebagai Kandidat Vaksin Myxobolusis [ Immune Response Analysis of Fish Koi (Cyprinus carpio Koi) Vaccinated Myxobolus Koi Spores Whole Protein as Vaccine Candidate Myxobolusis]

2019 ◽  
Vol 7 (1) ◽  
pp. 71
Author(s):  
Mohamad Yusuf ◽  
Gunanti Mahasri ◽  
Mufasirin Mufasirin
Keyword(s):  
Immune Response ◽  
Optical Density ◽  
Cyprinus Carpio ◽  
Vaccine Development ◽  
Fish Farming ◽  
Response Analysis ◽  
Randomized Design ◽  
Density Values ◽  
Completely Randomized Design ◽  
Cyprinus Carpio Koi

Abstract Myxobolus is one of parasites on koi fish that belongs to a class myxosporea that can infect and systemic and can cause harm to the fish farming. Vaccination is an attempt to cause-specific endurance through vaccination. Observations differential leukocytes and increased optical density values can be used to determine the effectiveness of the vaccine is given. This study aims to analyze the immune response koi fish vaccinated with Myxobolus koi spores whole protein for vaccine development myxobolusis in koi fish. The method used in this study is Completely Randomized Design with 4 treatments and 5 replications. The results showed that a change in the total number and types of leukocytes that can be used as indicators of the presence of certain infectious diseases that occure in fish. The highest value of lymphocytes in treatment B, monocytes highest in treatment D, neutrophils on treatment D, eosinophils on treatment A and basophils highest in treatment A. The observation of the highest optical density value in treatment B (fish vaccinated and infected 80 M. koi spores / tail) of 0.593 at day 30, while the lowest in treatment D (fish are not vaccinated but diinveksi 80 M. koi spores / tail) of 0,064 in 30 days

scholarly journals The Effect Of Rajalele Probiotic On Survival Rate Of Fish Juvenile Pangasius pangasius, Oreochromis niloticus, Cyprinus carpio

2020 ◽  
Vol 6 (1) ◽  
pp. 88-95
Author(s):  
Marlina Doloksaribu ◽  
Robert Martua Simanjuntak ◽  
Ied Hidayani Parinduri
Keyword(s):  
Social Sciences ◽  
Experimental Study ◽  
Data Analysis ◽  
Survival Rate ◽  
Cyprinus Carpio ◽  
Oreochromis Niloticus ◽  
Fish Farming ◽  
Randomized Design ◽  
The Social ◽  
Completely Randomized Design

The use of probiotics is certainly one of the solutions in overcoming the problem of increasing seed in fish farming. One of them is the use of probiotic king catfish, in this study tested on catfish, tilapia and goldfish. This research is an experimental study. The design used in this study was a Completely Randomized Design with 4 treatments 3 replications. Data analysis used Statistical Package for the Social Sciences version 15. The results of the analysis of the highest seed survival rate were obtained on Pangasius pangasius (95%), on Oreochromis niloticus (83,33 %), and Cyprinus carpio (80%). The treatment of Rajalele probiotics has a very significant effect (very significant) Fanalysis (23.01) > Ftable 0.01 (7.59) on the graduation of Pangasius pangasius, Oreochromis niloticus, and Cyprinus carpio. The conclusion of this study shows that the survival rate the highest in Pangasius pangasius

POTENSI TUMBUHAN BANGKAL (Nauclea Orientalis) UNTUK PENGENDALIAN BAKTERI Aeromonas Hydrophila (POTENTIAL PLANT BANGKAL (Nauclea orientalis) FOR CONTROL Aeromonas Hydrophila)

2016 ◽  
Vol 2 (4) ◽  
pp. 166
Author(s):  
Siti Aisiah
Keyword(s):  
Aeromonas Hydrophila ◽  
Fish Farming ◽  
Quality Parameters ◽  
Control Fish ◽  
Toxicity Tests ◽  
Distilled Water ◽  
Normal Range ◽  
Water Solvent ◽  
Randomized Design ◽  
Completely Randomized Design

Tujuan penelitian ini adalah didapatkan metode pengendalian penyakit yang disebabkan oleh bakteri Aeromonas hydrophila pada budi daya ikan yang ramah lingkungan. Dalam penelitian ini dilakukan uji sensitivitas terhadap bakteri A. hydrophila, uji minimal konsentrasi menghambat  bakteri A. hydrophila (uji MIC) dan uji toksisitas terhadap ikan nila. Rancangan  yang digunakan untuk  uji toksisitas  adalah rancangan acak lengkap (RAL), dengan 4 perlakuan yaitu A  = Ikan disuntik dengan ekstrak bangkal konsentrasi 20%, B  = Ikan disuntik dengan ekstrak bangkal konsentrasi 40%,  C  = Ikan disuntik dengan ekstrak bangkal konsentrasi 80%, dan D  = Kontrol (ikan tidak disuntik), diulang sebanyak 3 kali. Perlakuan ini didapat dari hasil uji sensitivitas antibakteri bangkal yang mempunyai daya hambat dan daya bunuh paling besar terhadap bakteri A. hydrophila yaitu ekstrak daun bangkal dengan pelarut akuades. Pengujian MIC menunjukkan bahwa ekstrak bangkal-akuades memiliki daya hambat minimal  20 % terhadap aktivitas bakteri A. hydrophila. Hasil uji toksisitas yang dilakukan terhadap ikan nila dengan konsentrasi 20%, 40% dan 80%  mortalitas ikan nila dibawah 50 %. Pengamatan hematologis yaitu eretrosit, leokosit, plasma darah, hematokrit dan leokokrit pada masing-masing perlakuan sebagian besar masih berada dalam kisaran yang normal. Parameter kualitas air yaitu, kadar oksigen terlarut,  pH, amoniak, CO2 dan suhu masih dapat mendukung kehidupan normal ikan nila.The purpose of this study was obtained method of controlling diseases caused by Aeromonas hydrophila in fish farming is environmentally friendly. In this study the sensitivity of the test  A. hydrophila, a minimum test konsentari inhibiting A. hydrophila (MIC test) and toxicity test on  tilapia. The design used for toxicity tests is completely randomized design with 4 treatments it A = Fish injected with extracts bangkal concentration of 20 %,  B = Fish injected with extracts bangkal concentration of 40 %, C = Fish injected with extracts of 80 % concentration bangkal, and D = Control ( fish not injected ), repeated 3 times. This treatment was obtained from the results of the sensitivity test antibacterial bangkal inhibition and has the power to kill most of the bacteria against A. hydrophila is bangkal leaf extract with distilled water solvent. MIC testing showed that the extract bangkal - distilled water has a minimum of 20 % inhibition of the bacterial activity A. hydrophila. Results of toxicity tests conducted on tilapia with a concentration of 20 %, 40 % and 80 % mortality of tilapia under 50 %. Haematological observation that eretrosit, leokosit, blood plasm, hematocrit and leokokrit in each treatment is still in the normal range. The water quality parameters, dissolved oxygen, pH, ammonia, CO2 and temperature can still support the normal life of tilapia.

scholarly journals Peningkatan Respon Imun Non-Spesifik Benih Kerapu Bebek, Cromileptes altivelis dengan Imunostimulan dan Bakterin terhadap Infeksi Viral Nervous Necrosis (VNN)

2006 ◽  
Vol 8 (1) ◽  
pp. 25
Author(s):  
Des Roza ◽  
Fris Johnny ◽  
Tridjoko Tridjoko
Keyword(s):  
Immune Response ◽  
Body Weight ◽  
Survival Rate ◽  
Intramuscular Injection ◽  
Survival Rates ◽  
Control Parameters ◽  
Treatment Results ◽  
Viral Nervous Necrosis ◽  
Randomized Design ◽  
Completely Randomized Design

An experiment to evaluate the effectiveness immunostimulant and bacterin on humpback grouper have been conducted at the Disease Laboratory of Research Institute for Mariculture, Gondol, Bali. The experiment was designed in completely randomized design with four treatments in duplicates. Two hundred of humpback grouper juveniles (15-18 cm of total length, 55-65 g of body weight) were injected intraperitoneally with (A) bacterine at 107 cfu/kg body weight (BW), (B) peptidoglycan at 100 mg/kg BW, and (C) immuno star at 100 ml/kg BW, (D) control. The fish were then challenged with VNN by intramuscular injection at 10 days post treatment. Results showed that survival rates of juvenile after challenged with VNN were 60.00% (B & C), 53.34% (A), and 1.67% (control). Parameters of non-spesific immune respons showed that phagocytic activities were 17.56% (B), 17.55% (C), 13.11% (A), and 9.33% (control). In addition, lisozyme activities were 1.64 cm (B), 1.58 cm (C), 1.55 cm (A), and 1.46 cm (control). Immunostimulant and bacterin stimulated non-specific immune response, and increased survival rate of humpback grouper juvenile.

scholarly journals Effect Of Commercial Biofertilizer Product Of Seaweed (Euchema Cottonii) Towards The Growth Of Pakcoy Mustard (Brassica Rapa L) and Dumbo Catfish (Clarias Gariepinus) in Aquaponic System

2020 ◽  
Vol 9 (2) ◽  
pp. 86
Author(s):  
Oktaviana Elsa Dewi ◽  
Sapto Andriyono ◽  
Mochammad Amin Alamsjah
Keyword(s):  
Survival Rate ◽  
Clarias Gariepinus ◽  
Fish Farming ◽  
African Catfish ◽  
Optimal Dosage ◽  
Vegetable Production ◽  
Randomized Design ◽  
Growing Medium ◽  
Completely Randomized Design ◽  
Biomass Plant

Aquaponics is a technology that combines the cultivation of vegetable production and fish farming. The aquaponics technology also to fertilization to give nutrients to the plant to support of survival. In facts, fertilization done to plants is located on land. Fertilizing the plants that live in the waters are still very rare, because the waters as a growing medium that is considered to always provide sufficient nutrients for plant growth. In this research, using biofertilizer Euchema cottonii of seaweed. The advantages of biofertilizer seaweed is to have the content of macro and micro nutrients are complete. It also contains substances hyper Grow (PGR) plant (auxin, cytokinin, gibberellin, abisat acid and ethylene).In this research to determine the effect of biofertilizer products of seaweed (E. cottonii) commercial on the growth of dumbo catfish (C. gariepinus) and pakcoy mustard (B. rapa L) on aquaponics system. The design of experiments in this research using a completely randomized design (CRD) with 5 treatments and 4 replications. The treatment used is fertilizer "AB mix®" 1% (A), without fertilizer (B) and the provision of products biofertilizer seaweed (E. cottonii) commercial 0,16% (C), 0,18% (D) and 0,20% (E). The main parameters measured were heavy growth of biomass, plant height pakcoy mustard (B. rapa L), growth SGR, length and survival rate of fish of dumbo catfish (C. gariepinus).The results showed that the dose of different commercial product biofertilizer seaweed (E. cottonii) gives a significantly different effect on the height growth of pakcoy mustard (B. rapa L) and the survival rate of dumbo catfish (C. gariepinus). The optimal dosage of commercial seaweed (E. cottonii) biofertilizer product in increasing the growth of pakcoy mustard (B. rapa L.) and African catfish (C. gariepinus) in the aquaponic system, namely treatment E at a dose of 0.20%.

scholarly journals PENGARUH PENCAMPURAN KOTORAN TERNAK SEBAGAI MEDIA KULTUR TERHADAP PERTAMBAHAN POPULASI CACING TANAH (Lumbricus rubellus)

2019 ◽  
Vol 34 (1) ◽  
pp. 75-80
Author(s):  
Satria Edi Putra ◽  
Iskandar Johan ◽  
Muhammad Hasby
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Fish Farming ◽  
Lumbricus Rubellus ◽  
Cattle Dung ◽  
Cow Dung ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Buffalo Dung ◽  
Growing Population

This research aims to determine the effect of mixing cattle dung as a culture medium to the growing population of earthworms. The research was conducted for 42 days starting from 18 October to 28 November 2017 at the fish farming school hall of Agriculture Faculty of Islamic University Riau Pekanbaru. The worm tree used in this study was as many as 960 tail or 80 tail for each container. Worms are maintained for 42 days with different mixtures of medium, i,e, 100% P1 dung treatment, P2 cow-dung treatment 50% + 50% buffalo dung, 50% cow dung + 50% goat dung, 50% cow dung + 50% chicken droppings. The research design was Completely Randomized Design with 4 treatments and 3 replications. The result of this research showed that the increase of population of earthworm with the mixing of cattle dung as culture media which conducted for 42 days is the best treatment of P2 as much as 4266 head/container, then the second one used was in treatment 3 as much as 1855 head/container, and the lowest increase of the population was at treatment P1 as much as 403 head/container, and followed by treatment P4 as much as 575 head/ container.

scholarly journals Biochemical and immune response in red tilapia (Oreochromis mossambicus × O. niloticus) with dietary chitosan supplementation

2021 ◽  
Vol 38 (4) ◽  
pp. 1016-1034
Author(s):  
Yuniel Méndez-Martínez ◽  
Ginger Pacheco-Morales ◽  
Karla Del Barco-Ibarra ◽  
Yenny Torres-Navarrete ◽  
Martha Hernández-Vergara
Keyword(s):  
Immune Response ◽  
Dry Matter ◽  
Oreochromis Mossambicus ◽  
Economic Losses ◽  
Red Tilapia ◽  
Promising Strategy ◽  
Fish Diets ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Different Levels

The use of biostimulants in fish diets is a promising strategy to reduce the use of antibiotics, enhance the biochemical and immune response, which contributes to improving productive yields and reducing economic losses. The biochemical and immune response was evaluated in juvenile red tilapia (Oreochromis mossambicus × O. niloticus), with different levels: 0 (control), 1, 2, 3, 4 and 5% chitosan in diet. 270 animals (7.53 ± 0.50 g of initial weight), distributed in a completely randomized design of 6 treatments with 3 replicates and 45 tilapia.treatment-1, were sown in 18 tanks with 90 L of water in a closed system. The juveniles were fed for 55 days with the experimental diets and at the end of the bioassay the tissue and blood plasma samples were taken. Differences were found (p<0.05) in the variables evaluated responses, where the content of lipids, triglycerides, cholesterol, carbohydrates and glucose showed higher levels (p<0.05) at lower chitosan concentrations. However, for protein content, a better response (p<0.05) was found at higher levels of chitosan in the diet. Regarding dry matter and ash, no differences were found (p˃0.05). While superoxide dismutase and leukocytes were found in higher concentration (p<0.05) when supplemented with chitosan by 3 and 4%. It was concluded that the juvenile biochemical and immune response of red tilapia was favored with dietary chitosan supplementation.

scholarly journals BIOKONTROL JENTIK NYAMUK Aedes aegypti DENGAN PREDATOR IKAN PEMAKAN JENTIK SEBAGAI PENDUKUNG MATERI AJAR INSEKTA

2012 ◽  
Vol 3 (1) ◽  
Author(s):  
Suharno Zen
Keyword(s):  
Aedes Aegypti ◽  
Dengue Fever ◽  
Cyprinus Carpio ◽  
Health Department ◽  
Mosquito Larvae ◽  
Mosquito Vector ◽  
Physical Chemical ◽  
Randomized Design ◽  
Natural Predator ◽  
Completely Randomized Design

Dengue fever is an infectious disease caused by a virus and is transmitted by the Aedes aegypti mosquito. Dengue fever has not yet found a cure and the only prevention is through mosquito vector  control. Health Department policy to prevent and eradicate dengue fever today is to make eradication by means of mechanical, physical, chemical or biological. Biological is one of alternative  control of mosquito vectors that are safe for humans and the environment but still effective in suppressing the mosquito vector. One attempt to do that is by using animals to fight other creatures, or better known as biological control. In the present research was performed using fish of Betta spp, Cyprinus carpio and Oreochormis niloticus as a natural predator of the Aedes aegypti mosquito. The design of this research is  Completely Randomized Design (CRD) with three treatments are 3 types of fish with 5 times repeated. Data were analyzed with analysis of the range and tested further with the Smallest Real Differences Test (LSD) on the real level 5%. The result of this research is the ability Betta spp eating is higher than the Oreochromis niloticus and Cyprinus carpio by the number of larvae that consumed between 34.6 to 36.9 mosquito larvae tail. The ability to eat at various types of fish against mosquito larvae, influenced by several factors: the agressive, espesialisasi fish to food, the active duration and the amount of time the fish is active in 24 hours.

scholarly journals EVALUASI PERTUMBUHAN BENIH PATIN (Pangasius hypophthalmus) YANG DIBERI PAKAN TAMBAHAN CACING SUTRA (Tubifex sp.)

2019 ◽  
Vol 6 (2) ◽  
pp. 77
Author(s):  
Dini Islama ◽  
Nurul Najmi
Keyword(s):  
Growth Rate ◽  
Survival Rate ◽  
Stocking Density ◽  
Fish Farming ◽  
Conversion Ratio ◽  
Feed Conversion Ratio ◽  
Culture Period ◽  
Feed Conversion ◽  
Randomized Design ◽  
Completely Randomized Design

This study aimed to evaluate of patin seed (Pangasius hypothalamus) that is given Tubifex sp. as additional feed. The experiment design was used  completely randomized design with four treatments and four replications. The treatments studied were 100 % pellet without the addition of Tubifex sp. (A), 95 % pellet with the addition of  Tubifex sp. 5 % (B), 90 % pellet with the addition of  Tubifex sp. 10 % (C) and 85 % pellet with the addition of  Tubifex sp. 15 % (D). Patin seed stocking density was 1 individu/l with the length of  3-4 cm and weight of  0,15-0,28 g. The culture period for one cycle of fish farming was 40 days. The ANOVA test showed commercial feeding with the addition of Tubifex sp. as additional feed gave the significant effect on the specific of growth rate, length and feed conversion ratio, but did not give the significant effect on survival rate of patin seed. The best dose to increase the growth of catfish seeds is 85% commercial feeding with the addition of Tubifex sp. 15%.

scholarly journals The Effectiveness of Seaweeds as Biofilter for Reducing Wastewater Nutrient and Preventing Water Pollution from Hybrid Grouper Culture

2021 ◽  
Vol 13 (2) ◽  
pp. 133
Author(s):  
Ratih Ida Adharini ◽  
Murwantoko Murwantoko ◽  
Namastra Probosunu ◽  
Riza Yuliratno Setiawan ◽  
Tony Budi Satriyo
Keyword(s):  
Aquatic Environment ◽  
Fish Farming ◽  
Treatment Technology ◽  
Randomized Design ◽  
Hybrid Grouper ◽  
Significant Difference ◽  
Improve Water Quality ◽  
Completely Randomized Design ◽  
Marine Fish Farming ◽  
Kjeldahl Nitrogen

Highlight ResearchWastewater in hybrid grouper culture should be maintain before resirculate and dispose to aquatic environment to avoid eutrophication.Seaweed that is used for biofilter must be observed for its suitability, performance and effectivity.Ulva sp. has the best effectiveness to reduce nutrient in wastewater of hybrid grouper aquaculture by absorbing then stored into thallus.Ulva sp. is proven has the best SGR and adaptability in wastewater of hybrid grouper culture.AbstractWastewater generated from hybrid grouper culture needs to be managed to improve water quality before being recirculated, or discharged in the aquatic environment. Seaweed biofilter has been proposed in wastewater treatment technology for marine fish farming. This study aimed at comparing which of these species i.e. Ulva sp., Sargassum sp., Gelidium sp., and Dictyota sp.  work best to absorb nutrient wastewater hybrid grouper culture. This research utilized these seaweed as treatments and controls with three replications using Completely Randomized Design. A statistical analysis was conducted to investigate the significant differences in the nutrient absorptions among various seaweed species by using ANOVA and least significant difference. Non-parametric tests namely Kruskal-Walis, Mann-Whitney, and t-test were used with confidence interval of 95%. The results revealed that Ulva sp. has the best ability to reduce the Nitrogen 80%, while Dictyota able to reduce  Phosphor 88% by Dictyota sp. generated from wastewater of hybrid grouper culture. While the highest absorbtion of Total Kjeldahl Nitrogen (104%) and phosphate (182%) that stored in thallus were performed by Ulva sp. Ulva sp. had the best performance and highest growth rate (1.9% d-1) as biofilter in hybrid grouper cultivation than other species.

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