PENGGUNAAN EKSTRAK DAUN SIRIH (Piper betle Linn) UNTUK MENGHAMBAT BAKTERI Aeromonas hydrophila DAN TOKSISITASNYA PADA IKAN PATIN (Pangasius hypophthalmus) (THE USE OF PIPER BETLE LINN EXTRACT ON AEROMONAS HYDROPHILA TO OBSTRUCT AND THE TOKSISITY TO PANGASIUS HYPOPHTHALMUS)

Tujuan penelitian ini untuk mengetahui daya hambat daun sirih yang paling besar terhadap bakteri Aeromonas Hydrophila, mengetahui konsentrasi minimal ekstrak daun sirih yang mampu menghambat pertumbuhan bakteri A. hydrophila dan mengetahui toksisitas konsentrasi efektif dari ekstrak daun sirih terhadap ikan patin. Rancangan percobaan yang digunakan untuk uji toksisitas adalah rancangan acak lengkap, terdiri dari 4 perlakuan yaitu A = Ikan disuntik dengan ekstrak daun sirih konsentrasi 75%, B = Ikan disuntik dengan ekstrak daun sirih konsentrasi 25%, C = Kontrol positif (ikan disuntik dengan akuades steril) dan D = Kontrol negatif (ikan tidak disuntik), diulang 3 kali. Hasil uji sensitivitas antibakteri daun sirih yang mempunyai daya hambat dan daya bunuh paling besar terhadap bakteri A. hydrophila adalah ekstrak daun sirih-metanol. Pengujian MIC menunjukkan bahwa ekstrak daun sirih-metanol memiliki daya hambat minimal 25 % terhadap aktivitas bakteri A. hydrophila. Hasil uji toksisitas yang dilakukan terhadap ikan patin dengan konsentrasi 75% dan 25% menunjukkan bahwa mortalitas yang terjadi tidak mencapai 50%. Pengamatan hematologis pada masing-masing perlakuan menunjukkan hasil yang berpengaruh tidak nyata terhadap kesehatan ikan patin. Parameter kualitas air pada penelitian ini yaitu, , kadar oksigen terlarut, pH, amoniak, CO2 dan suhu masih dapat mendukung kehidupan ikan patin.This research was aimed a finding the part of Piper betle Linn which had the biggest resistance to Aeromonas hydrophila bacteria and to know the minimal concentrate which could obstruct the growth of A. hydrophila bacteria and to know effective concentrate toxicity of P. betle Linn to Pangasius hypophthalmus. The random sampling used proportionate stratified random sampling. In toxicity test, it had be done 4 treatment, which was given to fish, those were : A = fish was injected with 25 % concentrate of extract P. betle Linn leaves, B = fish was injected with 75 % concentrate of extract P. betle Linn leaves, C = positive control (fish was injected with sterile aquadest), and D = negative control (fish wasn’t injected) and 3 trial. Sensivity result of A. hydrophila bacteria to P. betle Linn showed that the axtract of Piper betle Linn leaves-methanol had bigger activity than others. Depended on MIC test of the leaves P. betle Linn-methanol extract showed result that the extract had 25% minimal bloked concentrate to A. hydrophila bacteria. The result of toxicity test of P. betle Linn 75% and 25% leaves was no mortality 50% of P. hypophthalmus. Water quality parameter during experiment like dissolving oxygen, pH, ammonia, CO2, and temperature were still in reasonable range for Pangasius hypophthalmus.

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The Effectiveness of Turmeric Enriched Pellets in Curing the Gill of Clarias batrachus Infected with Aeromonas hydrophila

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/934/1/012006 ◽

2021 ◽

Vol 934 (1) ◽

pp. 012006

Author(s):

M Riauwaty ◽

Windarti

Keyword(s):

Aeromonas Hydrophila ◽

Curcuma Longa ◽

Histological Study ◽

Clinical Signs ◽

Positive Control ◽

Negative Control ◽

Infected Fish ◽

Clarias Batrachus ◽

Control Fish ◽

Gill Structure

Abstract Curcumin is an antibiotic agent extracted from Curcuma longa. To understand the effectiveness of curcumin in curing the gill of Clarias batrachus infected with Aeromonas hydrophila, a study was conducted from August to November 2020. There were 3 treatments applied, namely turmeric enriched pellets 0.7g/kg (P1); 0.9g/kg (P2) and 1.1g/kg (P3). Prior to the treatment, the fish was infected with A. hydrophila (0.1 m of A. hydrophila culture, 1.0x108cells/ml). The infected fish was kept for 6 days until the clinical signs of MAS disease occurred. By the 7th day, the fish was feed with turmeric enriched pellets. For negative control (Cn) the fish was not infected with A. hydrophila nor treated with turmeric, while the positive control (Cp) was infected, but no turmeric treatment. The fish was reared for 30 days and by the end of the experiment, the gill was removed and processed for histological study (5L sliced and HE stained). Result indicated that the gill of the negative control fish was normal, while that of the positive control shown abnormalities such as hemorrhages, necrotic cells, fused lamellae and enlarged cartilaginous cells. The turmeric treated fish shown less damage in the gill. The best results were obtained in C3, as the gill structure was almost normal with slightly hemorrhage. In the C1 and C2 treated fishes, the gill shown light abnormality such as fused lamella and hemorrhage. Data obtained shown that the consumption of turmeric enriched pellet was effective to cure the A. hydrophyla infected gill.

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Efikasi daun sembukan Paederia foetida untuk pencegahan infeksi bakteri Aeromonas hydrophila pada ikan nila

Jurnal Akuakultur Indonesia ◽

10.19027/jai.15.108-119 ◽

2016 ◽

Vol 15 (2) ◽

pp. 108 ◽

Cited By ~ 1

Author(s):

Dinamella Wahjuningrum ◽

Mulyati Hasanah ◽

, Rahman

Keyword(s):

Aeromonas Hydrophila ◽

Oreochromis Niloticus ◽

Preventive Treatment ◽

Positive Control ◽

Negative Control ◽

Optimum Dosage ◽

Coating Method ◽

Control And Prevention ◽

Paederia Foetida

ABSTRACT The purpose of this study was to determine the optimum dosage of skunkvine leaves Paederia foetida to prevent infection caused by bacteria Aeromonas hydrophila in tilapia Oreochromis niloticus. This study consisted of five treatments. They were negative control, positive control, and prevention treatment with the dosages of 0.8%, 1% and that 1.2% that consisted that of three replications in each treatment. Addition of skunkvine leaves on feed performed by coating method. Feed was given at satiation with a frequency of three times a day. The results of this study showed that there were significant effect (P<0.05) between the positive control (37.03%) and preventive treatment dosages of 0.8% (88.89%), 1% (74.08%), and 1.2% (74.08%). The optimum dosage for prevention of A. hydrophila infection in tilapia was 0.8%. Keywords: Aeromonas hydrophila, Paederia foetida, tilapia ABSTRAK Tujuan penelitian ini adalah untuk menentukan dosis daun sembukan Paederia foetida yang tepat dalam mencegah infeksi akibat Aeromonas hydrophila pada ikan nila Oreochromis niloticus. Penelitian ini terdiri dari lima perlakuan yaitu kontrol negatif, kontrol positif, perlakuan 0,8%, perlakuan 1%, dan perlakuan 1,2% dengan masing-masing perlakuan terdiri dari tiga ulangan. Metode penambahan sembukan pada pakan dilakukan dengan metode coating. Pakan diberikan secara at satiation dengan frekuensi pemberian sebanyak tiga kali sehari. Pemeliharaan ikan dilakukan selama 20 hari dan pada hari ke-11 dilakukan uji tantang dengan menggunakan bakteri A. hydrophila. Hasil penelitian ini menunjukan bahwa tingkat kelangsungan hidup sebelum uji tantang tidak berbeda nyata (P>0,05), sedangkan setelah uji tantang diperoleh hasil yang berbeda nyata (P<0,05) antara kontrol positif (37,03%) dengan perlakuan 0,8% (88,89%), perlakuan 1% (74,08%), dan perlakuan 1,2% (74,08%). Berdasarkan hasil penelitian disimpulkan bahwa pemberian daun sembukan melalui pakan efektif untuk pencegahan infeksi A. hydrophila pada ikan nila dengan dosis terbaik yaitu 0,8%. Kata kunci: Aeromonas hydrophila, Paederia foetida, ikan nila

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Hemostatic Effect of Ethanol Extract of Piper betle, Linn Leaves to Male Mice

Molekul ◽

10.20884/1.jm.2017.12.1.264 ◽

2017 ◽

Vol 12 (1) ◽

pp. 23

Author(s):

Sadakata Sinulingga ◽

Subandrate Subandrate ◽

Bebbi Arisya Kesumaputri ◽

Galuh Anggraini

Keyword(s):

Bleeding Time ◽

Ethanol Extract ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Piper Betle ◽

Control Group Design ◽

Minimum Concentration ◽

Group Design ◽

Hemostatic Effect

Hemorrhage occurs in most of the dental care. Untreated hemorrhage could cause excessive blood loss, hypotension, and cyanosis. A Natural resource that reported has an hemostatic effect is ethanol extract of betel leaves (Piper betel, Linn).The aim of this study is to find the minimum concentration of ethanol extract of betel leaves which capable of shortening the bleeding time in mice. The experimental study used pretest-posttest with control group design was conducted on 35 mice that divided into 7 group which are negative control, positive control (feracrylum 1%), the ethanol extract of betel leaves 1%, 5%, 10%, 15%, and 20%. All mice were injected heparin intravenously. Miceâs tail was cut at diameter 3 mm and pretest bleeding time was counted. Miceâs tail was recut at diameter 4 mm, given treatment for 5 seconds and posttest bleeding time was counted. Results of paired t-test showed that reduction of bleeding time between pretest and posttest was significant (p<0,050). The enhancement of ethanol extract of betel leaves concentration leads to better hemostatic effect. Results of ANOVA test showed that comparison of posttest bleeding time among groups was significant (p<0,050). The minimum concentration of ethanol extract of betel leaves which capable of shortening the bleeding time in mice is 5%.

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Phytochemical Constituents and Allelopathic Potential of Parthenium hysterophorus L. in Comparison to Commercial Herbicides to Control Weeds

Plants ◽

10.3390/plants10071445 ◽

2021 ◽

Vol 10 (7) ◽

pp. 1445

Author(s):

Mst. Motmainna ◽

Abdul Shukor Juraimi ◽

Md. Kamal Uddin ◽

Norhayu Binti Asib ◽

A. K. M. Mominul Islam ◽

...

Keyword(s):

Methanol Extract ◽

Positive Control ◽

Negative Control ◽

Parthenium Hysterophorus ◽

Allelopathic Effect ◽

Allelopathic Potential ◽

Glufosinate Ammonium ◽

Significant Difference ◽

Phytochemical Constituents ◽

Herbicide Glyphosate

The allelopathic effect of various concentrations (0, 6.25, 12.5, 50 and 100 g L−1) of Parthenium hysterophorus methanol extract on Cyperus iria was investigated under laboratory and glasshouse conditions. No seed germination was recorded in the laboratory when P. hysterophorus extract was applied at 50 g L−1. In the glasshouse, C. iria was mostly injured by P. hysterophorus extract at 100 g L−1. The phytochemical constituents of the methanol extract of P. hysterophorus were analyzed by LC-ESI-QTOF-MS=MS. The results indicated the presence of phenolic compounds, terpenoids, alkaloids, amino acids, fatty acids, piperazines, benzofuran, indole, amines, azoles, sulfonic acid and other unknown compounds in P. hysterophorus methanol extract. A comparative study was also conducted between P. hysterophorus extract (20, 40 and 80 g L−1) with a synthetic herbicide (glyphosate and glufosinate ammonium at 2 L ha−1) as a positive control and no treatment (negative control) on Ageratumconyzoides, Oryzasativa and C. iria. The growth and biomass of test weeds were remarkably inhibited by P. hysterophorus extract. Nevertheless, no significant difference was obtained when P. hysterophorus extract (80 g L−1) and synthetic herbicides (glyphosate and glufosinate ammonium) were applied on A.conyzoides.

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PEMANFAATAN PESTISIDA NABATI PADA PENGENDALIAN HAMA PLUTELLA XYLOSTELLA TANAMAN SAWI (Brassica juncea L.) MENUJU PERTANIAN RAMAH LINGKUNGAN

Jurnal Sains Dasar ◽

10.21831/jsd.v6i1.12998 ◽

2017 ◽

Vol 6 (1) ◽

pp. 36

Author(s):

Suhartini Suhartini ◽

Putu Suryadarma ◽

Budiwati Budiwati

Keyword(s):

Nicotiana Tabacum ◽

Brassica Juncea ◽

Plutella Xylostella ◽

Positive Control ◽

Negative Control ◽

Leaf Damage ◽

Piper Betle ◽

Randomized Design ◽

Biological Pesticide ◽

Chemical Pesticides

Abstrak Penggunaan pestisida kimia telah banyak memberikan dampak negatif pada lingkungan, sehingga diperlukan penggunaan pestisida nabati untuk menuju pertanian yang ramah lingkungan. Di desa ditemui banyak jenis daun yang dapat digunakan sebagai pestisida hayati, maka dari itu penelitian ini bertujuan untuk mengetahui efektivitas dari ekstrak pestisida nabati yaitu daun tembakau, tapak liman, daun kayu kuning dan daun sirih hijau terhadap mortalitas hama Plutella Xylostella pada tanaman sawi (Brassica juncea L.) berat basah sawi dan kerusakan daun sawi.Penelitian ini menggunakan Rancangan Acak Lengkap dengan perlakuan jenis tanaman sebagai pestisida Nabati . Perlakuan yang digunakan ada 6 macam (taraf) yaitu kontrol negatif (P0), daun tembakau (P1), tapak liman (P2), daun kayu kuning (P3), daun sirih hijau (P4) dan pestisida kimia sebagai kontrol positif (P5) dengan masing-masing kadar 10 %. Parameter yang diamati adalah mortalitas hama, berat basah sawi dan tingkat kerusakan daun sawi. Analisis dilakukan dengan anova satu arah.Hasil penelitian menunjukkan ekstrak daun tembakau ((Nicotiana tabacum), tapak liman ((Elephantopus sp.), daun kayu kuning (Arcangelisia flava L.) dan daun sirih hijau (Piper betle Linn.) dengan konsentrasi 10 persen dari ekstrak belum memberikan pengaruh nyata pada mortalitas hama Plutella xylostella, berat basah sawi ((brassica juncea l.) dan tingkat kerusakan daun sawi (brassica juncea l.). Ekstrak daun yang paling berpengaruh pada mortalitas Plutella xylostella secara berurutan adalah tembakau, daun sirih, daun kayu kuning dan tapak liman. Sedangkan pada berat basah sawi (brassica juncea l.) secara berurutan daun kayu kuning, daun sirih hijau, daun tapak liman dan daun tembakau. Sementara terhadap tingkat kerusakan daun sawi mulai yang paling kecil rusaknya secara berurutan adalah daun sirih hijau, daun tembakau, tapak liman dan daun kayu kuning. Kata Kunci: Pestisida Nabati, ekstrak, Plutella xylostella, mortalitas, sawi (Brassica juncea) ABSTRACTThe use of chemical pesticides has many negative impacts on the environment, it is necessary for the use of biological pesticide towards sustainable agriculture or environmentally friendly agriculture. In the village were encountered many types of leaves that can be used as a biological pesticide, and therefore this study aims to determine the effectiveness of pesticide plant extracts of the leaves of some plants covering the leaves of tobacco, elephantopus, yellow wood and green betel on mortality of Plutella xylostella pests in plants mustard greens (Brassica juncea L), heavy wet mustard and mustard leaf damage This study uses a completely randomized design with the treatment of various crops as a pesticide vegetable. The treatments used 6 kinds (degree) ie negative control (P0), the leaves of tobacco (P1), the leaves of elephantopus (P2), the leaves of yellow wood (P3), the leaves of greens betel (P4) and chemical pesticides as a positive control (P5) with each grade of 10 %. Parameters measured were mortality pests, heavy wet mustard greens and mustard greens leaf damage rate. The analysis was performed by one-way ANOVA. The results showed that the extract from the leaves of tobacco (Nicotiana tabacum), the leaves of elephantopus, the leaves of yellow wood (Arcangelisia flava L.) and the leaves of green betel (Piper betle Linn.) With a 10 percent concentration of the extract on a variety of leaves are not yet significant effect on mortality pests Plutella xylostella, heavy wet of mustard greens (brassica juncea l.) and severity of leaf mustard greens (brassica juncea l.). The leaf extract the most influence on mortality Plutella xylostella sequential is an extract of the leaves tobacco, the leaves of greens betel, the leaves of yellow wood and the leaves of elephantopus. While the effect on weight of wet mustard greens (Brassica juncea l.) in order are as follows: the leaves of yellow wood, the leaves of green betel, the leaves of elephantopus, and the leaves of tobacco. Meanwhile the level of damage to the mustard greens leaves are starting from the smallest breakdown in order are the leaves of green betel, the leaves of tobacco, the leaves of elephantopus and the leaves of yellow wood. Keywords: Pesticides Vegetable, extract, Plutella xylostella, mortality, Brassica juncea

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PENDAYAGUNAAN DUA JENIS ZINGIBERACEAE [C. mangga (temu mangga) dan K. angustifolia (kunci menir)] SEBAGAI SUMBER BAHAN IMUNOMODULATOR SECARA IN VITRO

Jurnal Teknologi Lingkungan ◽

10.29122/jtl.v11i3.1189 ◽

2016 ◽

Vol 11 (3) ◽

pp. 435

Author(s):

Praptiwi . ◽

Chairul .

Keyword(s):

Ethyl Acetate ◽

Methanol Extract ◽

Ethyl Acetate Fraction ◽

Positive Control ◽

Negative Control ◽

Phyllanthus Niruri ◽

Vitro Assay ◽

Macrophage Cells ◽

Almost All

Observation of imunomodulation properties of methanol extract derive (hexane,ethyl acetate, methanol/water) of Curcuma mangga (temu mangga) and Kaempferia angustifolia (kunci menir) had been carried out by in vitro assay. It was done by determining the phagocytised activity and capacity of macrophage cells of mice (Mus musculus) peritonium inducted with Staphyllococcus epidermidis. The result showed that each fraction had different phagocytised activity and capacity. Almost all of the fractions tested improved the activity (50–96 %) and capacity macrophage cells phagocytised compared to negative control (52 dan 502) and positive control (97 dan 1076). The positive control was 1000 ug Stimuno (Phyllanthus niruri extract), while the negative control was aquadest. The ethyl acetate fraction of Z. cassumunar (bangle) had highestphagocytised activity and capacity followed by C. mangga (temu mangga) and K. Angustifolia (kunci menir). It can be concluded that there were significant differences of phagocytised activity and capacity among fraction tested.Keywords : Zingiberaceae, Curcuma mangga, Kaempferia rotunda, Zingibercassumunar, immunomodulator, phagocytised, macrophage cells.

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INHIBITION TEST OF METHANOL EXTRACT FROM SOURSOP LEAF (Annona muricata Linn.) AGAINST Streptococcus mutans BACTERIA*

Jurnal Natural ◽

10.24815/jn.v17i1.6823 ◽

2017 ◽

Vol 17 (1) ◽

pp. 23 ◽

Cited By ~ 2

Author(s):

Raudhatul Jannah ◽

Muhammad Ali Husni ◽

Risa Nursanty

Keyword(s):

Dental Caries ◽

Streptococcus Mutans ◽

Methanol Extract ◽

Positive Control ◽

Negative Control ◽

Diffusion Method ◽

Annona Muricata ◽

High Prevalence ◽

Chemical Content ◽

And Mathematics

Abstract. Dental caries is a disease with a high prevalence of caries in Indonesia is caused by the bacterium Streptococcus mutans. The leaves of the soursop (Annona muricata Linn.) is one of the herbs that can remove dental plaque. This study aims to determine the chemical content and measure the inhibitory antibacterial soursop leaft methanol extractin inhibiting the growth of Streptococcus mutans bacteria. Phytochemical screening stated that the leaf of the soursop contains flavonoids, alkaloids, tannins, steroids, glycosides and saponins. Test of inhibition were measured using paper disc diffusion method with variation concentrations of 5, 10, 15 20, and 25%, positive control is tetrasilkin 30 µg, and a negative control is methanol. Antibacterial test results showed that the methanol extract of soursop leaves have antibacterial activity against Streptococcus mutans at concentrations of 5, 10, 15, 20 and 25% with inhibitory diameter of 9,1; 10,57; 11,53; 12,01 and 13,75 mm respectively. Keywords: Soursop leaves, Dental caries, Streptococcus mutans, Methanol.*Judul ini telah dipresentasikan pada Seminar Nasional: Indonesian Students Conference on Science and Mathematics (ISCSM) 11-12 November 2015, Banda Aceh Indonesia

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Short Communication: Hepatoprotective effect of methanol extract of sambiloto leaves (Andrographis paniculata) against malondialdehyde levels in liver tissues of paracetamol-induced Wistar rat

Nusantara Bioscience ◽

10.13057/nusbiosci/n100204 ◽

2018 ◽

Vol 10 (2) ◽

pp. 87-90

Author(s):

ANDRIANI ANDRIANI ◽

VIRHAN NOVRIANY ◽

GUNTUR SUSENO ◽

EFFIANA EFFIANA ◽

IIT FITRIANINGRUM

Keyword(s):

Normal Control ◽

Short Communication ◽

Methanol Extract ◽

Wistar Rat ◽

Positive Control ◽

Negative Control ◽

Andrographis Paniculata ◽

Hepatoprotective Effect ◽

Control Group ◽

Liver Tissues

Andriani, Suseno G, Effiana, Novriany V, Fitrianingrum I. 2018. Short Communication: Hepatoprotective effect of methanol extract of sambiloto leaves (Andrographis paniculata) against malondialdehyde levels in liver tissues of paracetamol-induced Wistar rat. Nusantara Bioscience 10: 87-90. Andrographis paniculata contains andrographolide and flavonoids, which have a hepatoprotective effect. This study was aimed to examine hepatoprotective effect of Andrographis paniculata extract against liver tissues malondialdehyde (MDA) levels of paracetamol induced Wistar rat (Rattus novegicus). The study was designed with posttest-only control group conducted at Tanjungpura University, Indonesia in June 2015. A total of 30 rats at age of three weeks were divided into 6 groups. They were given toxic dose paracetamol, curcumin and A. paniculata extract in different dose. Liver tissues were tested by Wills’s method. The data was analyzed using One-way ANOVA and LSD Post-hoc test. MDA levels of liver tissues were i.e, normal control (0.34 nmol mL-1), positive control (0.34 nmol mL-1), dose 1 (0.45 nmol mL-1), dose 2 (0.55 nmol mL-1), dose 3 (0.63 nmol mL- 1 ), negative control (1.32 nmol mL-1). Result showed that MDA levels of liver tissues of negative control group were significantly different with other groups (ANOVA, p≤ 0.05). There were no significant differences between MDA levels of liver tissues of Dose 1 with normal control and positive control (ANOVA, p>0.05). The methanol extract of A. paniculata has a hepatoprotective effect in (MDA) levels of paracetamol-induced Rattus novegicus with an effective dose of 500 mg kg-1.

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Potensi Antijamur Ekstrak Metanol Daun Mangrove Rhizopora Mucronata Terhadap Jamur Candida Albicans Dan Aspergillus Niger

JURNAL KIMIA MULAWARMAN ◽

10.30872/jkm.v15i1.583 ◽

2017 ◽

Vol 15 (1) ◽

pp. 29

Author(s):

Vega Julia Putri Adi ◽

Usman Usman

Keyword(s):

Candida Albicans ◽

Aspergillus Niger ◽

Methanol Extract ◽

Positive Control ◽

Negative Control ◽

Diffusion Method ◽

Rhizophora Mucronata ◽

Test Results ◽

Chemical Test ◽

X 24

Candida Albicans and Aspergillus Niger are some of the infectious fungi. Empirically bark, flower, root and mangrove leaves Rhizophora mucronata can be a hepatitis drug. This study aims to determine the antifungal potential of methanol extract of Rhizophora mucronata leaves to Candida Albicans and Aspergillus Niger fungus. Chemical test results that have been done previously are alkaloids, Flavanoid , triterpenoids, saponins, tannins and hydroquinone phenols. The antifungal test was performed by agar diffusion method on Potatos Dextrose Agar (PDA) medium and paper disc.Paper disc soaked in methanolic extract of Rhizophora mucronatapada leaf concentration 1000 ppm, 800 ppm, 600 ppm, 400 ppm and 200 ppm. The positive control solution used ketokenazole drug and negative control using aquades with incubation period of 3 x 24 hours. The results did not prove that Rhizophora mucronata leaf methanol extract was effective to inhibit antifungal activity against Candida Albicans and Aspergillus Niger fungi.

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UJI AKTIVITAS ANTELMINTIK EKSTRAK ETANOL DAUN SIRIH (Piper betle L.) TERHADAP CACING GELANG (Ascaris lumbricoides) SECARA IN VITRO

PHARMACON ◽

10.35799/pha.10.2021.34038 ◽

2021 ◽

Vol 10 (2) ◽

pp. 873

Author(s):

Priskila Feicy Sumual ◽

Widdhi Bodhi ◽

Julianri Sari Lebang

Keyword(s):

Ascaris Lumbricoides ◽

Anthelmintic Activity ◽

Ethanol Extract ◽

Positive Control ◽

Negative Control ◽

Piper Betle ◽

Group Design ◽

Significant Difference ◽

Post Test

ABSTRACTGreen betel leaves are one of the native plants in Indonesia which are widespread in Manado, North Sulawesi. In green betel leaves plants, there are tannin compounds that can inhibit enzymes and interfere with the digestive metabolic processes of worms which can cause the death of worms. This study aims to determine the effect of green betel leaves ethanol extract on Ascaris lumbricoides worms. This research is a laboratory experiment with The post-test only with controlled group design. The tests used the betel leaves ethanol extract with concentrations of 5%, 10%, and 15% respectively. Worms was observed for 12 hours with intervals of 3 hours. The number of worm deaths was recorded every 3 hours and further, it was analysed with using the Kruskal Wallis test and it was continued with using the Mann Whitney test. Result showed that extract at concentration of 5% the number of worm deaths was 4 worms, a concentration of 10% was 7 worms, and at a concentration of 15% 9 worms. The statistical results showed that there was no significant difference between the number of worm deaths in the treatment group and negative control at p <0.05. The concentrations of 10 and 15 showed no significant difference with the positive control. It can be concluded that concentrations of 10% and 15% have the same anthelmintic activity but the best concentration is at a concentration of 10%. Keywords: Anthelmintic, Piper betle L., Ascaris lumbricoides ABSTRAKDaun sirih hijau merupakan salah satu tanaman asli di Indonesia yang tersebar luas di kota Manado, Sulawesi Utara. Pada tumbuhan daun sirih hijau terdapat senyawa tanin yang mampu menghambat kerja enzim dan mengganggu proses metabolisme pencernaan pada cacing yang dapat menyebabkan kematian cacing. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak etanol daun sirih hijau terhadap cacing Ascaris lumbricoides. Penelitian ini ialah eksperimental laboratorium dengan rancangan penelitian The post-test only with controlled group design. Pengujian dilakukan menggunakan ekstrak etanol daun sirih dengan konsentrasi 5%, 10%, dan 15%. Aktivitas cacing diamati selama 12 jam dengan interval waktu 3 jam. Jumlah cacing yang mati dicatat setiap 3 jam dan selanjutnya dianalisis menggunakan uji Kruskal Wallis dan dilanjutkan dengan uji Mann Whitney. Hasil pengujian menunjukkan pada konsentrasi 5% jumlah kematian cacing sebanyak 4 ekor, konsentrasi 10% sebanyak 7 ekor dan pada konsentrasi 15% sebanyak 9 ekor. Hasil statistik menunjukkan tidak ada perbedaan signifikan antara jumlah kematian cacing pada kelompok perlakuan dengan kontrol negatif pada p<0.05. Konsentrasi 10 dan 15 menunjukkan tidak ada perbedaan signifikan dengan kontrol positif. Dapat disimpulkan bahwa pada konsentrasi 10% dan 15% memiliki aktivitas antelmintik yang sama namun konsentrasi yang paling baik terdapat pada konsentrasi 10%. Kata kunci: Antelmintik, Piper betle L., Ascaris lumbricoides

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