USE OF ANALYSIS TECHNIQUE FOR IMMOBILIZED YEAST SACCHAROMYCES CEREVISIAE IN THE BIOTECHNOLOGICAL INDUSTRY

2021 ◽  
pp. 94-109
Author(s):  
I. Bielykh ◽  
S. Samoilenko ◽  
A. Belinska ◽  
O. Varankina ◽  
O. Blyzniuk ◽  
...  
Keyword(s):  
Sodium Alginate ◽  
Immobilized Cells ◽  
Experimental Results ◽  
Biologically Active ◽  
Yeast Cells ◽  
Prolonged Storage ◽  
Dmso Solution ◽  
Cooling Mode ◽  
Slow Cooling ◽  
Alginate Solution

Article is devoted to the current state and problems of microbial cells immobilization and also prolonged storage of immobilized cells systems for the aims of biotechnological industry. In the experimental part immobilization conditions for the cells S. cerevisiae in alginate gel and vitality test, which had given high reproducibility of experimental results, were developed. Experimental results showed that viability of immobilized cells was higher than that of free yeast cells. It is possible that gel matrix has a protective effect on yeast cells during freezing. Comprehensive effect of cooling modes and preservation protective mediums, which contain sodium alginate, on viability of yeasts has been investigated. Advantage of yeast cells storage in immobilized state was shown experimentally. It was found that cooling mode and composition of preservation medium affect on the viability of S. cerevisiae cells during cryopreservation. In all freezing medium, both without protective components and with addition of a cryoprotective agent, the best results were obtained with cooling at a rate of 1°C/min. Viability indices in the samples were: 73.1 % – in distilled water; 90.8 % – in 1 % sodium alginate solution; 87.1 % – in 5 % DMSO solution and 86.1 % – in 1 % sodium alginate solution with the addition of 5 % DMSO. When cells were frozen in a 5 % DMSO solution and in a 1 % sodium alginate solution with the addition of 5 % DMSO, number of viable cells also decreased as cooling rate increased, but, probably, did not differ from the cell viability index in those samples that were frozen in 1 % sodium alginate solution. The highest results of viability for S. cerevisiae yeast cells were obtained during slow cooling for all cryoprotective mediums. For the first time, high cryoprotective properties of sodium alginate solution, were shown. Obtained results are enable to recommend the sodium alginate as a carrier for cryopreserved immobilized cells when using it in biotechnological processing for biologically active substances production.

scholarly journals Optimization of alcoholic fermentation using immobilized yeast cells in calcium alginate gel

2015 ◽  
pp. 207-218
Author(s):  
Jovana Djuran ◽  
Zorana Roncevic ◽  
Bojana Bajic ◽  
Sinisa Dodic ◽  
Jovana Grahovac ◽  
...  
Keyword(s):  
Sodium Alginate ◽  
Yeast Extract ◽  
Fossil Fuels ◽  
Alcoholic Fermentation ◽  
Immobilized Cells ◽  
Alginate Beads ◽  
Yeast Cells ◽  
Glucose Content ◽  
Alginate Concentration ◽  
Economic Analyses

Ethanol is an important industrial chemical with emerging potential as a biofuel to replace fossil fuels. In order to enhance the efficiency and yield of alcoholic fermentation, combined techniques such as cells immobilization and media optimization have been used. The aim of this study was the optimization of sodium alginate concentration and glucose and yeast extract content in the media for ethanol production with immobilized cells of Saccharomyces cerevisiae. Optimization of these parameters was attempted by using a Box-Behnken design using the response surface methodology. The obtained model predicts that the maximum ethanol content of 7.21% (v/v) is produced when the optimal values of sodium alginate concentration and initial content of glucose and yeast extract in the medium are 22.84 g/L, 196.42 g/L and 3.77 g/L, respectively. To minimize the number of yeast cells "eluted" from the alginate beads and residual glucose content in fermented media, additional two sets of optimization were made. The obtained results can be used for further techno-economic analyses of the process to select the optimum conditions of the fermentation process for industrial application.

scholarly journals Mead Production Using Immobilized Cells of Saccharomyces cerevisiae: Reuse of Sodium Alginate Beads

Processes ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 724
Author(s):  
Miguel L. Sousa-Dias ◽  
Vanessa Branco Paula ◽  
Luís G. Dias ◽  
Letícia M. Estevinho
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Sodium Alginate ◽  
Fermentation Process ◽  
Immobilized Cells ◽  
Alginate Beads ◽  
Production Medium ◽  
Alcohol Content ◽  
Volatile Acidity ◽  
The Matrix ◽  
Second Category

This work studied the production of mead using second category honey and the immobilized cells of Saccharomyces cerevisiae in sodium alginate, with concentrations of 2% and 4%, and their reuse in five successive fermentations. The immobilized cells with 4% alginate beads were mechanically more stable and able to allow a greater number of reuses, making the process more economical. The fermentation’s consumption of sugars with free cells (control) and immobilized cells showed a similar profile, being completed close to 72 h, while the first use of immobilized cells finished at 96 h. The immobilized cells did not significantly influence some oenological parameters, such as the yield of the consumed sugars/ethanol, the alcohol content, the pH and the total acidity. There was a slight increase in the volatile acidity and a decrease in the production of SO2. The alginate concentrations did not significantly influence either the parameters used to monitor the fermentation process or the characteristics of the mead. Mead fermentations with immobilized cells showed the release of cells into the wort due to the disintegration of the beads, indicating that the matrix used for the yeast’s immobilization should be optimized, considering the mead production medium.

scholarly journals Evaluation of Dynamic Properties of Sodium-Alginate-Reinforced Soil Using A Resonant-Column Test

Materials ◽  
2021 ◽  
Vol 14 (11) ◽  
pp. 2743
Author(s):  
Seongnoh Ahn ◽  
Jae-Eun Ryou ◽  
Kwangkuk Ahn ◽  
Changho Lee ◽  
Jun-Dae Lee ◽  
...  
Keyword(s):  
Shear Modulus ◽  
Sodium Alginate ◽  
Shear Failure ◽  
Dynamic Properties ◽  
Damping Ratio ◽  
Reinforced Soil ◽  
Resonant Column ◽  
Column Test ◽  
Alginate Solution ◽  
Resonant Column Test

Ground reinforcement is a method used to reduce the damage caused by earthquakes. Usually, cement-based reinforcement methods are used because they are inexpensive and show excellent performance. Recently, however, reinforcement methods using eco-friendly materials have been proposed due to environmental issues. In this study, the cement reinforcement method and the biopolymer reinforcement method using sodium alginate were compared. The dynamic properties of the reinforced ground, including shear modulus and damping ratio, were measured through a resonant-column test. Also, the viscosity of sodium alginate solution, which is a non-Newtonian fluid, was also explored and found to increase with concentration. The maximum shear modulus and minimum damping ratio increased, and the linear range of the shear modulus curve decreased, when cement and sodium alginate solution were mixed. Addition of biopolymer showed similar reinforcing effect in a lesser amount of additive compared to the cement-reinforced ground, but the effect decreased above a certain viscosity because the biopolymer solution was not homogeneously distributed. This was examined through a shear-failure-mode test.

Natural Stilbene Polyphenols as Yarrowia lipolytica Cell Cytoprotectors at Heat Stress

2021 ◽  
Vol 37 (6) ◽  
pp. 14-24
Author(s):  
N.N. Gessler ◽  
E.P. Isakova ◽  
Yu.I. Deryabina
Keyword(s):  
Heat Stress ◽  
Heat Shock ◽  
Yarrowia Lipolytica ◽  
Respiration Rate ◽  
Catalase Activity ◽  
Thermal Exposure ◽  
Biologically Active ◽  
Yeast Cells ◽  
Protective Properties ◽  
Cyanide Resistance

Using the extremophilic yeast of Yarrowia lipolytica, a new model has been proposed to study the protective properties of stilbene polyphenols, namely resveratrol and pinosylvin, under heat shock. It was shown that a short-term thermal exposure of yeast cells (55 C, 25 min) led to a 40% decrease in the colony-forming ability of the population, a fivefold decrease in the respiration rate, and a growth of cyanide resistance and catalase activity, which indicated the adaptive yeast response to heat stress. Under these conditions, natural biologically active stilbenes, resveratrol and pinosylvin, at a concentration of 10 μM each increased yeast survival by 28% and 13%, respectively. In heat shock, resveratrol additionally raised catalase activity, while pinosylvin increased the cell respiration rate and decreased cyanide resistance and catalase activity. The results obtained indicate that resveratrol acts as a mild pro-oxidant inducing antioxidant protection during the adaptive response of the yeast to heat shock. Unlike resveratrol, pinosylvin increases cell survival stabilizing mitochondrial function and preserving the ATP-generating component of respiration. Yarrowia lipolytica yeast, polyphenols, stilbenoids, resveratrol, pinosylvin, cellular respiratory activity, heat shock, superoxide dismutase, catalase

Stability of Anthocyanin from Mulberry Extracts in Alginate Microspheres at High Temperature

2012 ◽  
Vol 506 ◽  
pp. 587-590 ◽  
Author(s):  
R. Yamdech ◽  
Pornanong Aramwit ◽  
Sorada Kanokpanont
Keyword(s):  
High Temperature ◽  
Sodium Alginate ◽  
Dry Weight ◽  
Flow Rates ◽  
Alginate Solution ◽  
Water Extracts ◽  
External Gelation ◽  
Mulberry Fruit ◽  
Total Anthocyanins ◽  
Mulberry Fruits

The aim of this study was to investigate stabilities of anthocyanins from mulberry fruits extracts in alginate microspheres. The total anthocyanins contents measured from water extracts of mulberry fruits (Chiangmai/Jul cultivars) were at 10.46±0.51 mg/g DW and 15.31±0.86 mg/g DW (Chiangmai/Korat cultivars). At high temperature the extracts lost their anthocyanin contents to 52%wt (at 100°C for 5 hour) and 73%wt (at 121°C for 20 min). Alginate microspheres were prepared using external gelation methods. Sodium alginate solution (2.5%w/v) was sprayed into CaCl2solution (0.1M) through a nozzle at N2flow rates of 20 L/min. The average bead sizes were at 342.03±3.21µm. Anthocyanins loading on alginate microspheres were at 2.283±0.15 mg/g dry weight. At 80(10h), 100(5h) and 121°C(20min), Anthocyanins retention were at 91%wt, 82%wt and 89%wt, respectively. Adsorption of anthocyanins from mulberry fruit extracts on alginate microspheres resulted in a greater stability at high temperature than that of the free anthocyanins.

scholarly journals Structural and mechanical characteristics of some lead-free Cu-Sn based solder alloys

2008 ◽  
Vol 62 (3) ◽  
pp. 160-163 ◽  
Author(s):  
Aleksandra Mitovski ◽  
Ljubisa Balanovic ◽  
Dragana Zivkovic ◽  
Sasa Marjanovic ◽  
Bata Marjanovic ◽  
...  
Keyword(s):  
Mechanical Characteristics ◽  
Dendritic Structure ◽  
Production Method ◽  
Experimental Results ◽  
Lead Free ◽  
Solder Alloys ◽  
Slow Cooling ◽  
Mechanical And Electrical Properties ◽  
Additional Thermal Treatment ◽  
Lead Free Solders

The results of structural and mechanical characteristics of lead-free Cu-Sn based solder alloys, produced in Company "11. mart" AD Srebrenica (Republic of Srpska), are presented in this paper. The results of investigation of samples - alloys CuSnl4, CuSnlFelAlO.5, CuSnlOFelAllMnO.5 and CuA110Fe3Mn produced by different processing methods, include the data obtained by optical microscopy and measurements of hardness, micro hardness and electroconductivity, in order to characterize mentioned alloys and define the influence of processing method applied on their structural and mechanical properties. Microstructural experimental results of samples produced by casting in a metal mould with fast water cooling showed clearly sharp dendritic structure. Samples obtained by casting in a sand mould, displayed structure with big crystals, higher amount of segregation and inclusions on the grain boundaries, as a result of the slow cooling process. Hardness and microhardness tests showed increasing values as the amount of tin raised. Sample 3 showed the lowest value, as a result of the crystallization process and lackness of additional thermal treatment. Experimental results of the electroconductivity test showed that mentioned sample has got the highest value, which can be also explained by its production method. Results presented in this paper can contribute to investigations of copper-tin lead-free alloys, having in mind that various potential lead-free solders still haven't been completely investigated from the aspects of their structural, mechanical and electrical properties.

scholarly journals Processes of heat and mass transfer during drying of red beetroot

2020 ◽  
Vol 6 (2) ◽  
pp. 81-87
Author(s):  
Zhanna Petrova ◽  
◽  
Kateryna Samoilenko ◽  
Vitaly Vishnevsky
Keyword(s):  
Mass Transfer ◽  
Energy Consumption ◽  
Heat And Mass Transfer ◽  
Raw Materials ◽  
Antioxidant Properties ◽  
Calculation Model ◽  
Experimental Results ◽  
Biologically Active ◽  
Raw Material ◽  
Drying Process

Red beetroot is the main raw material which has a high content of betanine with antioxidant properties. An important emphasis in the processing of antioxidant raw materials by drying is to reduce energy consumption for the dehydration process, the maximum preservation of biologically active substances, and to reduce the cost of the final product. Drying is a complex and energy-intensive process. Therefore, to optimize energy consumption during drying and selection of rational modes of dehydration, it is necessary to apply the calculated analysis of heat and mass transfer on the basis of adequate mathematical models. Calculated and experimental results are compared. In general, the comparison of the results of numerical modeling of convection drying processes of the red beetroot sample with the experimental results showed their rather satisfactory qualitative agreement. The calculation model can be used to approximate the characteristics of the drying process of red beetroot, in particular the time required for drying. The obtained results of calorimetric studies allow stating that with correctly selected compositions, not only the components of native raw materials are stabilized, but also the drying process is intensified with the reduction of energy consumption to process.

Study on the Calamine/Sodium Alginate Modified Viscose Fiber

2011 ◽  
Vol 418-420 ◽  
pp. 192-195
Author(s):  
Dong Qi Liu ◽  
Ying Liu ◽  
Shu Fa Han ◽  
Yu Feng Zhang ◽  
Cui Yu Yin
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Sodium Alginate ◽  
Mechanical Performance ◽  
Viscose Fiber ◽  
Alginate Solution ◽  
Fiber Properties ◽  
Injection Methods ◽  
The Stability ◽  
Scanning Electron

In this article we successfully prepared calamine / sodium alginate viscose fiber. Good dispersion and stability of the modified solution was prepared by dispersing calamine in alkaline solution of sodium alginate, and then mixed it with viscose spinning solution by spinning injection methods. Moreover, the stability of calamine / sodium alginate solution, the effect of concentration of calamine on the fiber properties is studied in this paper. Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and physical mechanical performance are test to characterize the structure and the performance of the calamine / sodium alginate viscose fiber.

Sodium Alginate Solution Transferred by Laser Induced Forward Transfer

2019 ◽  
Vol 48 (1) ◽  
pp. 114002
Author(s):  
王文兵 WANG Wen-bing ◽  
郭钟宁 GUO Zhong-ning ◽  
于兆勤 YU Zhao-qin ◽  
朱紫红 ZHU Zi-hong ◽  
邓宇 DENG Yu
Keyword(s):  
Sodium Alginate ◽  
Alginate Solution ◽  
Forward Transfer

Experimental Study on the Adsorption of MB on Novel Photocatalyst Yeast/ZnS Hybrid Microspheres

2014 ◽  
Vol 924 ◽  
pp. 129-133
Author(s):  
Hai Gang Gou ◽  
Wei Sheng Guan ◽  
Li Yang ◽  
Zhao Qi Wang ◽  
Yang Zhi Zhu
Keyword(s):  
Experimental Study ◽  
Methylene Blue ◽  
Adsorption Capacity ◽  
Experimental Results ◽  
Yeast Cells ◽  
Isotherm Modeling ◽  
Adsorption Performance

Employing yeast cells as carriers,a novel cell-loaded photocatalyst yeast/ZnS (YC/ZnS) was successfully synthesized. The adsorption performance of methylene blue on the YC/ZnS microspheres was studied. Experimental results showed that the optimized adsorption conditions were 23°C,pH7 and the YC/ZnS dosage 4.5g/L. Under these conditions,the adsorption capacity of YC/ZnS could reach 471.2μg/g. Isotherm modeling revealed that Freundlich equations described well the adsorption of methylene blue onto YC/ZnS.

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