Effect of treatment with sodium hydroxide on the feeding value of oat and rape straw for sheep

1985 ◽  
Vol 36 (4) ◽  
pp. 623 ◽  
Author(s):  
JB Coombe ◽  
JG Mulholland ◽  
RI Forrester
Keyword(s):  
Organic Matter ◽  
Fatty Acid ◽  
Volatile Fatty Acid ◽  
Rape Straw ◽  
Rumen Ph ◽  
Volatile Fatty Acid Concentration ◽  
N Utilization ◽  
Feeding Value ◽  
Naoh Treatment

Oat and rape straws were treated with NaOH (70 and 40 g kg-1, respectively) by spraying with NaOH solutions (20 or 12.5% w/w), consolidating the sprayed straw into stacks, and storing under plastic. After storage for up to 60 weeks, in vitro digestibilities had been approximately doubled, although it appeared that the reaction was complete by 4 weeks. Potential digestibility of straw organic matter estimated by a polyester bag technique, was increased by c. 0.1 by NaOH treatment but the rate of digestion in the rumen was not changed. Following storage, treated and untreated straws were dried, ground, mixed with supplements and pelleted for feeding to sheep. Mixtures of treated or untreated oat straw, minerals, c. 3% urea and 0, 14, 28 or 42% starch were fed to mature wethers. The main effects of NaOH treatment of straw were to increase water intake, digestibilities of organic matter and cellulose, and rumen volatile fatty acid levels, and decrease rumen ammonia levels. Low intakes of NaOH-straw with no added starch were associated with high rumen pH (> 7.5 , probably due to unreacted alkali (equivalent to 23 g NaOH kg-1). Inclusion of starch, but not NaOH-straw treatment, improved dietary N utilization, although mean N balances were negative (- 5.5 to - 0.1 g day-1) on all diets. Feeding treated straw increased the amounts of Na eaten, and of Na and P apparently absorbed; the extra absorption was accounted for by increased urinary excretion so that retention was not increased. When diets of rape straw supplemented with urea and minerals were fed to 2-year-old wethers, NaOH treatment of straw increased intakes of food and water, organic matter digestibility, rumen volatile fatty acid concentration and N balance.

scholarly journals USING SAGO PITH AS ADHESIVE SUBSTANCE IN VITRO DIGESTIBILITY OF COMPLETE RATION BISCUIT

2020 ◽  
Vol 4 (1) ◽  
pp. 91-101
Author(s):  
Shirley Fredriksz ◽  
Lily Joris
Keyword(s):  
Organic Matter ◽  
Fatty Acid ◽  
Rice Bran ◽  
Volatile Fatty Acid ◽  
In Vitro Digestibility ◽  
Elephant Grass ◽  
Total Volatile ◽  
Rumen Ph ◽  
Total Volatile Fatty Acid

The objective of the study was to determine in-vitro digestibility of complete ration biscuit using sago pith as adhesive substance. The experiment was arranged in Complete Randomized Design with 5 treatments and 5 replications. The treatments were P1 (50% mini elephant grass+ 20%  leucaena + 30% sago pith + 0% rice bran), P2 (50% mini elephant grass+ 20%  leucaena + 25% sago pith + 5% rice bran), P3 (50% mini elephant grass+ 20%  leucaena + 20% sago pith +10% rice bran ) and P4 (50% mini elephant grass+ 20%  leucaena + 15% sago pith + 15% rice bran). Variables measured were digestibility of dry (DM) and organic matter (OM), NH3 level, total volatile fatty acid (VFA), and rumen pH. The results show that the treatment has no significant effect on vitro digestibility of dry and organic matter. In vitro -digestibility is ranging from   90.7 – 91.4% and 87.5 – 88.4 % for DM and OM, respectively. In-vitro test on complete ration biscuit which consists of sago pith, rice bran, mini elephant grass and leucaena leucocephala has no significant  (P>0.05) on NH3 level, total volatile fatty acid and rumen pH. In conclusion, sago pith can be potentially used as adhesive substance in formulating complete ration biscuit.  

scholarly journals The Effect of Multistage Ammoniation on Fiber Fraction and Digestibility of Maize Stover in vitro

2016 ◽  
Vol 5 (1) ◽  
Author(s):  
K. Dakaew ◽  
A. Abrar ◽  
A. Cherdthong
Keyword(s):  
Organic Matter ◽  
Fatty Acid ◽  
Volatile Fatty Acid ◽  
Dry Matter ◽  
Total Volatile ◽  
Maize Stover ◽  
Dry Matter Digestibility ◽  
Fiber Fraction ◽  
Total Volatile Fatty Acid

The main objective of this experiment was to assess the effect of multistage ammoniation on fiber fraction and digestibility of maize stover in vitro. Maize stover was treated by a multistage technique of different urea connect ration (8%, 4%, and 2%) and incubation for 14 days at room temperature.Digestibility of NDF, ADF, ADL, hemicellulose, and cellulose, in vitro dry matter digestibility (DMD), organic matter digestibility (OMD), NH3-N, total volatile fatty acid (VFA) and pH concentration were observed. However treatment maize stover and multistage ammoniation maize stover were composed by using T-test student as the statistic. There were not significantly different on fiber fraction and digestibility of maize stover. However, multistage ammoniation decreased ADF fraction by 56.67% to45.39%. The DM digestibility of multistage ammoniation on fiber fraction and digestibility of maize stover also increased by 35.18 to 45.91. The total VFA and N-ammonia of multistage ammoniation on fiber fraction and digestibility of maize stover also higher than control.Keywords: Ammoniation, Digestibility, in vitro, Maize stover

Essential oils from Lippia turbinata and Tagetes minuta persistently reduce in vitro ruminal methane production in a continuous-culture system

2019 ◽  
Vol 59 (4) ◽  
pp. 709 ◽  
Author(s):  
F. Garcia ◽  
P. E. Vercoe ◽  
M. J. Martínez ◽  
Z. Durmic ◽  
M. A. Brunetti ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Essential Oils ◽  
Acid Concentration ◽  
Methane Production ◽  
Volatile Fatty Acid ◽  
Crude Protein ◽  
Gas Production ◽  
Volatile Fatty Acid Concentration ◽  
Tagetes Minuta

The aim of the present study was to evaluate the impact of essential oils (EO) from Lippia turbinata (LT) and Tagetes minuta (TM) as well as the rotation of both EO on fermentation parameters in vitro. Daily addition of LT, TM, or a 3-day rotation between them (TM/LT), as well as a control (without EO), was evaluated using the rumen simulation technique (Rusitec). The experiment lasted 19 days, with a 7-day adaptation period, followed by 12 days of treatment (Days 0–12). The EO were dissolved in ethanol (70% vol/vol) to be added daily to fermenters (300 μL/L) from Day 0. Daily measurements included methane concentration, total gas production, apparent DM disappearance and pH, which started 2 days before the addition of treatments. On Days 0, 4, 8 and 12 apparent crude protein disappearance and neutral detergent fibre disappearance, ammonia and volatile fatty acid concentration and composition were determined. Methane production was significantly inhibited shortly after addition of both EO added individually, and persisted over time with no apparent adaptation to EO addition. The TM/LT treatment showed a similar effect on methane production, suggesting that rotating the EO did not bring further improvements in reduction or persistency compared with the inclusion of the EO individually. Gas production, total volatile fatty acid concentration and composition and apparent crude protein disappearance were not affected by EO addition. Compared with the control, a 5% reduction of apparent DM disappearance and a 15% reduction of neutral detergent fibre disappearance were observed with the addition of EO. Only TM and TM/LT reduced ammonia concentration. Given the significant and persistent antimethanogenic activity of both EO, and the potential of T. minuta to modify nitrogen metabolism, EO from these plant species are of interest for developing new feed additives with potential application in ruminant nutrition that are also likely to be acceptable to consumers.

The effect of barley supplements on the voluntary intake and digestion of low quality roughages by sheep

1979 ◽  
Vol 92 (1) ◽  
pp. 235-241 ◽  
Author(s):  
C. S. Lamb ◽  
J. Eadie
Keyword(s):  
Organic Matter ◽  
Volatile Fatty Acid ◽  
Latin Square ◽  
Molar Proportion ◽  
Rumen Ph ◽  
Volatile Fatty Acid Concentration ◽  
Acid Detergent Fibre ◽  
Oat Straw ◽  
Ad Libitum ◽  
Voluntary Intake

SummaryOat straw, timothy straw and two field-cured hays (43·8, 47·3, 52·0 and 56·0% organic matter digestibility (OMD) and 0·56, 0·75, 1·00 and 1·41% N respectively) were fed ad libitum together with 0, 235, 470 and 705 g D.M. of rolled barley to 5-year-old North Country Cheviot wethers in four 4x4 Latin square designs (Expt 1). In an attendant study (Expt 2) of conditions with in the rumen four rumen fistulated wethers were offered daily 0, 200, 400 and 600 g D.M. of rolled barley with ad libitum hay (54·3% OMD, 1·45% N), also following a Latin square design.The voluntary intake of two of the hays (54·3 and 56·0% OMD) decreased progressively as the barley increased. Intakes of the other roughages were slightly increased when the lowest amount of barley was fed. For each roughage total organic-matter intake and the OMD of the ration increased progressively with increasing amount of barley consumed. No associative effects of barley on the OMD of the roughages were demonstrated. The acid-detergent fibre (ADF) digestibility of diets comprising the highest amount of barley with the oat straw and the field-cured hay of lowest N content were significantly lower than that of the respective all-roughage feeds. In Expt 2 the intake of increasing amounts of barley significantly reduced rumen pH, the molar proportion of acetic acid and the disappearance of hay and hay ADF from terylene bags within the rumen, while volatile fatty acid concentration and the molar proportion of butyric acid increased.Multiple regressions were obtained relating the change in roughage intake to the amount of barley fed and the digestibility and chemical composition of the roughages. The equation with the least error, explaining 88% of the variation, was Y= 30·561 — 0·615C — 21·453N±3·69, where Y is the change in roughage intake as a percentage of the intake of roughage fed alone, Cis barley intake as g OM/W0·73 and N is the nitrogen content of the roughage.

scholarly journals Pengaruh Imbangan Jerami Padi, Dedak Padi dan Onggok Terfermentasi terhadap Kecernaan dan Produk Fermentasi Rumen Secara In Vitro

2010 ◽  
Vol 10 (2) ◽  
pp. 59-63
Author(s):  
S.N.O. Suwandyastuti ◽  
Efka Aris Rimbawanto ◽  
Ning Iriyanti
Keyword(s):  
Organic Matter ◽  
Fatty Acid ◽  
Rice Straw ◽  
Rice Bran ◽  
Volatile Fatty Acid ◽  
Dry Matter ◽  
Industrial Wastes ◽  
Optimal Ratio ◽  
Dry Matter Digestibility

The influence of the ratio of fermented rice straw, rice bran and cassava solid waste upon the In vitro digestibility and rumen fermentation productABSTRACT. Chemical and physical treatments have been attempted to improve the utilization of agro industrial wastes, but the result is not efficient and caused pollution. Besides, biological treatments using the microbes have been used to improve the nutritive value and utilization of agro industrial wastes. The current experiment was conducted to find out the optimal ratio of fermented rice straw, fermented rice bran and fermented tapioca waste. There were five kinds of ratio of fermented rice straw, fermented rice bran and fermented tapioca waste, namely: K1 (70% fermented rice straw + 15% fermented rice bran + fermented tapioca waste), K2 (60% fermented rice straw + 20% fermented rice bran + 20% fermented tapioca waste), K3 (50% fermented rice straw + 25% fermented rice bran + 25% fermented tapioca waste), K4 (40% fermented rice straw + 30% fermented rice bran + 30% fermented tapioca waste) and K5 (30% fermented rice straw + 35% fermented rice bran + 35% fermented tapioca waste) An in vitro technique, using completely Randomize Block Design was applied and each treatment was repeated four times. Variables measured were Dry matter and Organic matter digestibility and rumen fermentation products (volatile fatty acid and N-NH3 concentration). The dry matter digestibility of K1, K2, K3, k4 and K5 was 29.39, 31.27, 32.33, 33.71 and 34.82%, respectively. The organic matter digestibility of K1, K2, K3, K4 and 5 was 30.82, 31.27, 32.73, 34.94, and 34.92, respectively. Volatile fatty acid concentrations of K1, K2, K3, K4 and K5 were 95.19, 91.77, 87.21, 104.31, 106.59 mM/l, respectively. N-NH3 concentrations of K1, K2, K3, K4 and K5 were 0.97, 0.93, 0.93, 1.00, 1.04 mM/l, respectively. Significant difference (P0.01) was only found in dry matter digestibility among treatments, while others variables were not significantly different among treatments. It was indicated that the optimal ratio was K4.

scholarly journals KAJIAN IN VITRO SUBTITUSI KONSENTRAT DENGAN PENGGUNAAN LIMBAH PERKEBUNAN SINGKONG YANG DISUPLEMENTASI KOBALT (Co) DAN SENG (Zn) DALAM RANSUM DOMBA

2015 ◽  
Vol 39 (2) ◽  
pp. 71
Author(s):  
Iman Hernaman ◽  
Atun Budiman ◽  
Siti Nurachman ◽  
Kundrat Hidrajat
Keyword(s):  
Organic Matter ◽  
Fatty Acid ◽  
Volatile Fatty Acid ◽  
In Vitro Study ◽  
Normal Range ◽  
Native Grass ◽  
Concentrate Supplementation ◽  
Randomized Design ◽  
Completely Randomized Design

<p>This research was aimed to investigate the effect of concentrate substitution with cassava plantation waste supplemented with cobalt and zinc in sheep ration. In vitro study was used in this experiment. Collected data were analyzed by Duncan’s test from Completely Randomized Design with four treatments and four replications. The experiment rations were R1 = 50% native grass + 50% concentrate, R2 = 50% native grass + 50% concentrate + 5 ppm cobalt + 30 ppm zinc, R3 = 50% native grass + 50% cassava plantation waste, R4 = 50% native grass + 50% cassava plantation waste + 5 ppm cobalt + 30 ppm zinc. The results showed that utilization of concentrate increased volatile fatty acid and N-NH3 concentration, and digestibility of dry and organic matter compared with cassava plantation waste. Volatile fatty acid and N-NH3 in sheep ration containing cassava plantation waste were still in normal range with dry and organic matter digestibility were up to 50%, but supplementation of cobalt and zinc had the same effect (P&gt;0.05). It is concluded that cassava plantation waste can be used as sheep feed, but did not substitute concentrate. Supplementation of cobalt and zinc was not effective to improve fermentability and digestibility of cassava plantation waste.</p><p><br />(Key words: Cassava plantation waste, Digestibility, In vitro, N-NH3, Sheep, Volatile fatty acid)</p>

Determining the nutritive value of some commercial pulse screenings using in sacco and in vitro methods

2020 ◽  
Vol 60 (3) ◽  
pp. 379
Author(s):  
Ghodrat Mohammadi ◽  
Mostafa Malecky ◽  
Jamal Seifdavati
Keyword(s):  
Fatty Acid ◽  
Volatile Fatty Acid ◽  
Nutritive Value ◽  
Gas Production ◽  
Total Volatile ◽  
Volatile Fatty Acid Concentration ◽  
Ruminal Degradability ◽  
Total Volatile Fatty Acid ◽  
In Sacco

The present study aimed at determining in sacco and in vitro the nutritive value of lentil screenings (LS), common vetch screenings (CVS) and bitter vetch screenings (BVS). For this purpose, three experiments (Exp.) were conducted. Ruminal degradation characteristics of the screenings were determined in Exp. 1 by the nylon bag technique using three ruminally fistulated rams. Gas production kinetics, and ruminal degradability and fermentation indices of the screenings were determined using 144 and 24-h incubations, respectively in Exp. 2. In Exp. 3, a basal growing ration for lambs (control) was supplemented by 175 or 350 g/kg of either LS, CVS or BVS (LS175, LS350, CVS175, CVS350, BVS175 and BVS350 respectively), and ruminal degradability and fermentation of the rations were evaluated in vitro. Soluble and potentially degradable fractions of protein were 0.321 and 0.719, 0.362 and 0.688, and 0.333 and 0.707, for LS, BVS and CVS respectively. Metabolisable energy was 11.1, 11.6 and 12.1 MJ/kg DM for LS, BVS and CVS respectively. The screenings had comparable gas production profiles, ruminal digestibility and total volatile fatty acid concentration. However, CVS and BVS had a higher propionate and a lower acetate:propionate ratio than LS (P &lt; 0.05). Including BVS and LS in the mixed ration increased total volatile fatty acid at both inclusion levels, but only BVS enhanced propionate proportion and lowered the acetate:propionate ratio (P &lt; 0.05). These results revealed that these screenings have a good nutritional value and can be used in ruminant diets without adversely affecting the rumen fermentation.

scholarly journals In Vitro Rumen Simulations Show a Reduced Disappearance of Deoxynivalenol, Nivalenol and Enniatin B at Conditions of Rumen Acidosis and Lower Microbial Activity

Toxins ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 101 ◽  
Author(s):  
Sandra Debevere ◽  
An Cools ◽  
Siegrid De Baere ◽  
Geert Haesaert ◽  
Michael Rychlik ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Acid Production ◽  
Volatile Fatty Acid ◽  
Rumen Fluid ◽  
Fatty Acid Production ◽  
Lactating Cows ◽  
Rumen Ph ◽  
Rumen Acidosis ◽  
Better Than

Ruminants are generally considered to be less susceptible to the effects of mycotoxins than monogastric animals as the rumen microbiota are capable of detoxifying some of these toxins. Despite this potential degradation, mycotoxin-associated subclinical health problems are seen in dairy cows. In this research, the disappearance of several mycotoxins was determined in an in vitro rumen model and the effect of realistic concentrations of those mycotoxins on fermentation was assessed by volatile fatty acid production. In addition, two hypotheses were tested: (1) a lower rumen pH leads to a decreased degradation of mycotoxins and (2) rumen fluid of lactating cows degrade mycotoxins better than rumen fluid of non-lactating cows. Maize silage was spiked with a mixture of deoxynivalenol (DON), nivalenol (NIV), enniatin B (ENN B), mycophenolic acid (MPA), roquefortine C (ROQ-C) and zearalenone (ZEN). Fresh rumen fluid of two lactating cows (L) and two non-lactating cows (N) was added to a buffer of normal pH (6.8) and low pH (5.8), leading to four combinations (L6.8, L5.8, N6.8, N5.8), which were added to the spiked maize substrate. In this study, mycotoxins had no effect on volatile fatty acid production. However, not all mycotoxins fully disappeared during incubation. ENN B and ROQ-C disappeared only partially, whereas MPA showed almost no disappearance. The disappearance of DON, NIV, and ENN B was hampered when pH was low, especially when the inoculum of non-lactating cows was used. For ZEN, a limited transformation of ZEN to α-ZEL and β-ZEL was observed, but only at pH 6.8. In conclusion, based on the type of mycotoxin and the ruminal conditions, mycotoxins can stay intact in the rumen.

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