Protective Effects of Rheum Turkestanicum Janischagainst Diethylnitrosamine-Induced Hepatocellular Carcinoma in Rats

Abstract Aim of the study Hepatocellular carcinoma (HCC) is common cancer that causes many deaths worldwide. Recent studies have reported anti-cancer effects of R. turkestanicum against various cell lines including leukemia cervical tumor, and breast cancer. In this study, we aimed to identify the effect of R. turkestanicum against diethylnitrosamine (DEN)-induced HCC. Methods Wistar rats were divided into four groups of control, DEN, DEN + 100 mg/kg or 400 mg/kg of hydroethanolic extract of the plant roots. Results After four months, the animals in the DEN group showed HCC foci in the liver, an increase of hepatic lipid peroxidation, attenuation of hepatic antioxidant capacity, an increase of blood liver enzymes (ALT, AST, and ALP), bilirubin, albumin, creatinine, glucose, and reduction of the body weight. The plant extract could decrease the levels of liver enzymes, total bilirubin, direct bilirubin, albumin, urea, and creatinine in the blood. Also, the extract attenuated oxidative stress and improved pathological changes in the liver. Quantitative real-time PCR revealed a decrease in gene expression of Wnt/β-catenin and Akt and an increase in PTEN as the tumor suppressor gene. Conclusion The extract of R. turkestanicum reduced DEN-induced liver changes through inhibiting oxidative stress and attenuating the expression of Wnt/β-catenin and Akt and elevating PTEN expression.

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The antitoxic and protective effects of Curcuma longa and it`s active agent, Curcumin: оverview

Terapevt (General Physician) ◽

10.33920/med-12-2006-09 ◽

2020 ◽

pp. 66-72

Author(s):

A. Khisamova ◽

O. Gizinger

Keyword(s):

Curcuma Longa ◽

Physiological Activity ◽

Active Agent ◽

Modern Science ◽

Physical Exertion ◽

The Body ◽

Modern World ◽

Protective Effects ◽

Daily Stress ◽

Anti Cancer

In the modern world, where a person is exposed to daily stress, increased physical exertion, the toxic effect of various substances, including drugs. The task of modern science is to find antioxidants for the body. These can be additives obtained both synthetically and the active substances that we get daily from food. Such a striking example is turmeric, obtained from the plant Curcuma longa. Recently, it has been known that curcumin has an antioxidant, anti-inflammatory, anti-cancer effect and, thanks to these effects, plays an important role in the prevention and treatment of various diseases, in particular, from cancer to autoimmune, neurological, cardiovascular and diabetic diseases. In addition, much attention is paid to increasing the biological activity and physiological effects of curcumin on the body through the synthesis of curcumin analogues. This review discusses the chemical and physical characteristics, analogues, metabolites, the mechanisms of its physiological activity and the effect of curcumin on the body.

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Protective Effect of Hydroethanolic Extracts of Solanum scabrum and Cola verticillata Against Cyclophosphamide Induced Toxicity in Female Rats

Journal of Food Research ◽

10.5539/jfr.v3n3p18 ◽

2014 ◽

Vol 3 (3) ◽

pp. 18 ◽

Cited By ~ 1

Author(s):

Mbong Angie M-A ◽

Djiokeng Paka G. ◽

Ntentie F. R. ◽

Dimodi H. ◽

Ngondi J. L. ◽

...

Keyword(s):

Oxidative Stress ◽

Intraperitoneal Administration ◽

Female Rats ◽

Protective Effects ◽

Oxidative Stress Markers ◽

Stress Markers ◽

Significant Toxicity ◽

Cyclophosphamide Treatment ◽

Hydroethanolic Extract ◽

Antioxidant Mechanisms

The aim of this study was to evaluate the protective effects of hydroethanolic extracts of S. scabrum and C. verticillata against cyclophosphamide induced toxicity. In this light, female albino wistar rats were treated by intraperitoneal administration of 100 mg/kg BW of cyclophosphamide or distilled water every other day for 7 days associated with oral gavage using hydroethanolic extract of C. verticillata/S. scabrum at a dose of 200 or 400 mg/kg BW or not every day for the same 7 days. On the 8th day, blood and organs (liver, heart and kidney) were collected for analyses of toxicity-related and oxidative stress markers. Cyclophosphamide treatment induced significant toxicity as shown by liver enzymes, urea and creatinine levels. The administration of extracts helped reduce the levels of these markers. The antioxidant effect of these extracts also helped or not to ameliorate oxidative stress markers (MDA, NO, hydroperoxides, catalase, thiols, GPx) depending on the extract and on the dose administered. These results suggest that administration of hydroethanolic extracts of S. scabrum and C. verticillata can help prevent or reduce toxicity that is brought about by treatment with cyclophosphamide due to their ability to upregulate antioxidant mechanisms.

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Anti-Inflammatory and Antioxidative Effects of Sumatriptan Against Doxorubicin-Induced Cardiotoxicity in Rat

ACTA MEDICA IRANICA ◽

10.18502/acta.v59i7.7020 ◽

2021 ◽

Author(s):

Mohammad Sheibani ◽

Hedyeh Faghir-Ghanesefat ◽

Yaser Azizi ◽

Tahmineh Mokhtari ◽

Hasan Yousefi‐Manesh ◽

...

Keyword(s):

Oxidative Stress ◽

Body Weight ◽

Mortality Rate ◽

Cardiac Tissue ◽

The Body ◽

Male Rats ◽

Protective Effects ◽

Cardiac Damage ◽

Necrosis Factor Alpha ◽

Antioxidative Effects

The clinical use of doxorubicin as a potent chemotherapeutic agent is limited due to its dose-dependent cardiotoxicity. Oxidative stress and inflammatory pathways have a pivotal role in doxorubicin-induced cardiotoxicity. Sumatriptan, a 5-hydroxytryptamine (5-HT)1B/1D agonist that is mainly used to relieve migraine pain, has suggested exerting protective effects in numerous pathological conditions through antiinflammatory properties. The aim of the present study was to investigate the effects of sumatriptan on doxorubicin-induced cardiotoxicity and the contribution of anti-inflammation and antioxidative responses. Cardiotoxicity was induced by the administration of doxorubicin three times a week (2.5 mg/kg i.p) for two consecutive weeks on male rats. The animals were divided into four groups, including Control, Sumatriptan (0.1 mg/kg) received group, doxorubicin received group, and Doxorubicin+Sumatriptan (0.1 mg/kg) received group. Sumatriptan was administered 30 min before every injection of doxorubicin. On the last day of the second week, the body weight, mortality rate, electrocardiogram (ECG) and histopathological changes, cardiac inotropic study, and biochemical factors were evaluated. The loss of body weight, mortality rate, ECG parameters, reduction of papillary muscle contractility force as well as histopathological scores following administration of doxorubicin indicated severe cardiac damage. However, treatment with sumatriptan inhibited the functional and structural impairment induced by doxorubicin. In addition, sumatriptan could significantly reduce cardiac tissue levels of malondialdehyde (MDA) and tumor necrosis factor-alpha (TNF-α), which were increased in the doxorubicin-treated rats. This study illustrated the protective effects of sumatriptan on decreasing doxorubicin-induced cardiac toxicity and mortality rate in part through inhibition of inflammatory and oxidative stress pathways.

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Oxidative Stress and Inflammation Induced by Waterpipe Tobacco Smoking Despite Possible Protective Effects of Exercise Training: A Review of the Literature

Antioxidants ◽

10.3390/antiox9090777 ◽

2020 ◽

Vol 9 (9) ◽

pp. 777 ◽

Cited By ~ 1

Author(s):

Behzad Taati ◽

Hamid Arazi ◽

Katsuhiko Suzuki

Keyword(s):

Oxidative Stress ◽

Exercise Training ◽

Tobacco Smoking ◽

Relaxation Method ◽

The Body ◽

Protective Effects ◽

Respiratory Problems ◽

Risk Of Mortality ◽

Waterpipe Tobacco Smoking ◽

The Impact

The prevalence of waterpipe tobacco smoking (WTS), which is also known as ghalyan, shisha or hookah, is increasing rapidly around the world, especially among youth. Growing interest in this form of tobacco smoking can be traced, in part, to the use of flavored tobacco products, social acceptability as a safer option than cigarettes, and its consideration as a relaxation method or entertainment. However, there is a well-established association between WTS and oxidative stress that causes irreversible chronic pathological conditions such as cardiovascular and respiratory problems, as well as different types of cancers, and thus increases the risk of mortality. Clearly, induction of inflammation status through increased reactive oxygen species (ROS), which in turn leads to oxidative stress and harm to lipids, DNA, and proteins, is the most plausible mechanism to explain the potential harmful effects of WTS. Unlike WTS, well-designed exercise training programs increase ROS to the extent that it is beneficial to the body. In this study, we aimed to review available evidence on the impact of exercise training on oxidative stress and inflammation status. We also summarize the effect of acute and chronic WTS on different exercise capacities.

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The Protective Effects of Turmeric on Liver Enzymes of Metronidazole-Treated Adult Male Wistar Rats

International Journal of Advances in Scientific Research ◽

10.7439/ijasr.v1i6.2214 ◽

2015 ◽

Vol 1 (6) ◽

pp. 255

Author(s):

Akudike C. J. ◽

Chukwu V. O. ◽

Ezejindu D. N. ◽

Ihim A. C.

Keyword(s):

Adult Male ◽

Wistar Rats ◽

Liver Enzymes ◽

Antimicrobial Effect ◽

The Body ◽

Protective Effects ◽

Negative Effects ◽

Serum Samples ◽

Male Wistar Rats ◽

Anti Oxidant

Over the years, research works on the different liver enzymes has proven to be very helpful to man. This study evaluates the protective effects of turmeric on the liver enzymes of metronidazole-treated adult male wistar rats. With previous researches, metronidazole has been found to exert some negative effects on some organs of the body like the testis, kidney etc but the turmeric on its own part, has been found to have no side effects with a host of beneficial functions such as its anti-oxidant and antimicrobial effect amongst a host of other functions. In this study, twenty wistar rats with weight range of between 165-180g were assigned into four groups A, B, C and D of 5 each. The experimental groups A, B and C were orally administered 200mg of metronidazole, 400mg of metronidazole and 400mg of metronidazole as well as 400mg of turmeric respectively for duration of twenty-eight days while the group D served as control and were orally administered water and feed only. Twenty four hours after the last administration, the animals were anaesthetized under chloroform inhalation and dissected for organ collection. Blood for serum preparation was collected into sterile plain tubes and stored in the refrigerator for analysis. Serum samples from the bloods were analyzed for liver enzymes activities using randox kit method. The study revealed that turmeric helped reduced the effects of metronidazole on the serum activities of ALT, AST and ALP on the liver. This present study suggests that metronidazole could have negative effects on the liver and so advises that turmeric be added when on metronidazole administration.

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Oxidative Stress Management in Chronic Liver Diseases and Hepatocellular Carcinoma

Nutrients ◽

10.3390/nu12061576 ◽

2020 ◽

Vol 12 (6) ◽

pp. 1576 ◽

Cited By ~ 1

Author(s):

Daisuke Uchida ◽

Akinobu Takaki ◽

Atsushi Oyama ◽

Takuya Adachi ◽

Nozomu Wada ◽

...

Keyword(s):

Oxidative Stress ◽

Hepatocellular Carcinoma ◽

Liver Disease ◽

Viral Hepatitis ◽

Liver Diseases ◽

Chronic Viral Hepatitis ◽

Chronic Liver ◽

Chronic Liver Diseases ◽

Anti Cancer ◽

The Impact

Chronic viral hepatitis B and C and non-alcoholic fatty liver disease (NAFLD) have been widely acknowledged to be the leading causes of liver cirrhosis and hepatocellular carcinoma. As anti-viral treatment progresses, the impact of NAFLD is increasing. NAFLD can coexist with chronic viral hepatitis and exacerbate its progression. Oxidative stress has been recognized as a chronic liver disease progression-related and cancer-initiating stress response. However, there are still many unresolved issues concerning oxidative stress, such as the correlation between the natural history of the disease and promising treatment protocols. Recent findings indicate that oxidative stress is also an anti-cancer response that is necessary to kill cancer cells. Oxidative stress might therefore be a cancer-initiating response that should be down regulated in the pre-cancerous stage in patients with risk factors for cancer, while it is an anti-cancer cell response that should not be down regulated in the post-cancerous stage, especially in patients using anti-cancer agents. Antioxidant nutrients should be administered carefully according to the patients’ disease status. In this review, we will highlight these paradoxical effects of oxidative stress in chronic liver diseases, pre- and post-carcinogenesis.

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Blueberry Polyphenol Extracts Enhance the Intestinal Antioxidant Capacity in Weaned Rats by Modulating the Nrf2–Keap1 Signal Pathway

Frontiers in Physiology ◽

10.3389/fphys.2021.640737 ◽

2021 ◽

Vol 12 ◽

Author(s):

Fangfang Zhao ◽

Shen Yan ◽

Mengliang Tian

Keyword(s):

Oxidative Stress ◽

Antioxidant Capacity ◽

Gene Transcription ◽

Interleukin 1 ◽

Antioxidant Properties ◽

The Body ◽

Initiation Factor ◽

Standard Diet ◽

Related Factor ◽

Protective Effects

Weaning causes the generation of excessive reactive oxygen species in the body, which could lead to oxidative stress. Polyphenols, for which blueberries are an important dietary source, are known for various health benefits including antioxidant properties. Here, we sought to elucidate the effects of blueberry polyphenol extracts (BPE) on intestinal antioxidant capacity and possible underlying mechanisms in weaned rats. Ninety-six rats were assigned to two groups and fed either a standard diet or a standard diet supplemented with BPE (200 mg/kg). The results showed that BPE supplementation increased (P < 0.05) catalase and superoxide dismutase activities and decreased (P < 0.05) interleukin-1 and interferon-γ contents in the jejunum and ileum. The abundances of mammalian target of rapamycin, ribosomal p70 S6 kinase and eukaryotic initiation factor 4E-binding protein 1 mRNA were elevated in the jejunum and ileum (P < 0.05) after BPE supplementation. Additionally, BPE supplementation decreased (P < 0.05) Kelch-like ECH-associated protein 1 (Keap1) gene transcription and enhanced (P < 0.05) NF-E2-related factor 2 (Nrf2) gene transcription in the jejunum and ileum. According to our results, BPE-induced protective effects against oxidative stress appear through the promotion of the jejunal and ileal antioxidant defense system in weaned rats, which was associated with the Nrf2–Keap1 signaling pathway.

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Quercetin and curcumin effects in experimental pleural inflammation

Medicine and Pharmacy Reports ◽

10.15386/mpr-1484 ◽

2020 ◽

Author(s):

Cristina Bidian ◽

Daniela-Rodica Mitrea ◽

Olivia Gabriela Vasile ◽

Adriana Filip ◽

Adriana Florinela Cătoi ◽

...

Keyword(s):

Oxidative Stress ◽

Experimental Studies ◽

The Body ◽

Male Rats ◽

Protective Effects ◽

Control Groups ◽

Beneficial Effects ◽

Pulmonary Pathology ◽

Anti Inflammatory ◽

Antioxidant Effects

Background. The inflammatory mechanisms occur with the highest prevalence in pulmonary pathology. In pharmaceutical industry, carrageenan is used as a pro-inflammatory agent when the activity of anti-inflammatory agents is tested. The oxidative stress represents the imbalance between pro-oxidants and antioxidants which can lead to the activation of the oxidative mechanisms with noxius potential to the body. In experimental studies, quercetin is the most active flavonoid, having the highest anti-inflammatory and antioxidant effects. Curcumin has antioxidant effects that are similar to those of the standard antioxidants and exerts direct anti-inflammatory activity. Aims. The aim of this study is to determine the antioxidant effects of quercetin and curcumin on a carrageenan-induced pleural inflammation. Methods. Eight groups of adult male rats were used: Ia and Ib -control groups, IIa and IIb -with carrageenan administration, IIIa and IIIb -with curcumin and carrageenan, IVa and IVb -with quercetin and carrageenan administration. Blood and lung samples were taken at 4 hours (Ia, IIa, IIIa, IVa groups) and at 24 hours (Ib, IIb, IIIb, IVb groups) after carrageenan administration. Results. In serum, at 4 and at 24 hours, curcumin and quercetin showed protective effects, reducing the oxidative stress (malondialdehyde significantly decreased) and stimulating the antioxidant protection (ceruloplasmin and glutathione significantly increased) in rats with administration of these substances, in comparison to the group that received only carrageenan. In the lungs, at 4 hours, the oxidative stress was significantly reduced only in the rats that received quercetin (malondialdehyde significantly decreased), modifications that were not observed at 24 hours. Conclusions. In serum, curcumin presented higher antioxidant effects, compared to quercetin. In lungs, quercetin administration showed superior beneficial effects, but only temporarily.

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Effect of cyclophosphamide on hematological and physiological and possible protective role of Berberis vulgaris in mice

10.36811/ijbm.2021.110022 ◽

2021 ◽

pp. 1-7

Author(s):

Idriss H. Mohamed

Keyword(s):

Oxidative Stress ◽

White Blood Cells ◽

Protective Role ◽

Physiological Parameters ◽

Protective Effects ◽

Distribution Width ◽

Lung Cancers ◽

Berberis Vulgaris ◽

Anti Cancer ◽

Hematological And Biochemical Parameters

Aim of study to investigate the effect of certain natural products from medicinal plants as adjuvant systems to reduce toxicity of chemotherapy. cyclophosphamide (CTX) is anti-cancer DNA alkylating chemotherapeutic agent to act against a variety of tumors in head and neck, testicular, ovarian, bladder, small-cell lung cancers, this study performed to investigate the effect of Berberis vulgaris in combination with CTX on hematological and physiological parameters induced by CTX in mice. In this study we evaluate the possible protective effects of Berberis vulgaris on hematological and physiological parameters in mice chronically treated with CTX. Four groups of mice were examined: a control (I), mice treated with CTX (II), mice treated with CTX + Berberis vulgaris (III), and mice treated with Berberis vulgaris (IV). All animals were treated for successively 5 days and killed one week after the last treatment, The results show significant decreases in levels of red blood cell distribution width (RDW), white blood cells (WBCs), neutrophils and lymphocytes counts with increases in levels of monocytes. Also, hepatocytes oxidative stress which characterized by significant increases in the serum activities aspartate aminotransferase (AST), alanine aminotransferase (ALT). Berberis vulgaris combined with CTX or Berberis vulgaris alone successfully normalized the hematological and biochemical parameters in form returning (RDW), (WBCs), monocytes and lymphocytes count to normal level. Hepatocytes oxidative stress, which characterized by a significant decrease in the serum activities of (AST) and (ALT). Keywords: Cyclophosphamide; Berberis vulgaris; Lymphocytes; Hepatocytes; Significant

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Antioxidant Properties of Resveratrol and its Protective Effects in Neurodegenerative Diseases

Advances in Cell Biology ◽

10.2478/acb-2014-0006 ◽

2014 ◽

Vol 4 (2) ◽

pp. 97-117 ◽

Cited By ~ 26

Author(s):

Joanna Gerszon ◽

Aleksandra Rodacka ◽

Mieczysław Puchała

Keyword(s):

Oxidative Stress ◽

Insulin Resistance ◽

Organic Compound ◽

Neurodegenerative Diseases ◽

Antioxidant Properties ◽

Cardiovascular Effects ◽

Protective Effects ◽

Therapeutic Drugs ◽

Anti Cancer ◽

Natural Organic Compound

Summary Resveratrol is a natural organic compound, polyphenol, produced naturally by some plants in response to several harmful factors such as attack by pathogens, UV radiation, or increased oxidative stress. Many experiments suggest that it triggers mechanisms that counteract aging-related effects and plays a role in insulin resistance as well. It also possesses beneficial properties such as anti-cancer, anti-inflammatory, blood-sugar-lowering and cardiovascular effects. It is supposed to exhibit an interesting activity in neuroprotection - mainly through activation of sirtuins and counteraction in forming peptide aggregates. Still research is needed to evaluate exactly how resveratrol protects neurons, and to develop new, potential, therapeutic drugs

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