scholarly journals The Effects of IFN-γ and IL-4 Expression on PCNA Expression in Transplanted Bone Tumors

2021 ◽  
Author(s):  
Jiaxing Lv ◽  
Li Zhao ◽  
Leipeng Bai ◽  
Ying Xu ◽  
Yu Jin ◽  
...  
Keyword(s):  
Bone Tumor ◽  
Bone Tumors ◽  
Tumor Tissue ◽  
Tumor Model ◽  
Expression Level ◽  
Control Group ◽  
P Value ◽  
Tumor Implantation ◽  
Ifn Γ ◽  
Experimental Group

Abstract Objective: The rabbit VX2 bone tumor model is an ideal experimental animal model for studying malignant bone tumors, but few studies on cytokines and tumor proliferation. This paper is aimed to study the effect of interferon-γ (IFN-γ) and interleukin-4 (IL-4) on the expression of proliferating cell nuclear antigen (PCNA) in tumor tissue. Methods: The experiment included 30 Japanese white rabbits divided into an experimental group (n=15) and a control group (n=15) according to the random number method. Peripheral blood and tumor tissue were collected at 1 and 2 weeks after tumor implantation. We used enzyme-linked immunosorbent assay to detect the expression levels of IFN-γ and IL-4 in peripheral blood and PCNA in tumor tissue. After the rabbits were euthanasia, the tissues were taken for pathological examination. Results: After tumor implantation, the expression level of IFN-γ in the experimental group in the 2nd week was significantly lower than that in the 1st week and 2nd week in the control group (p-value<0.005). In contrast, the expression level of IL-4 in the experimental group in the 2nd week was significantly higher than that in the 1st week and 2nd week in the control group (p-value<0.005). The expression level of PCNA in the experimental group in the 2nd week was significantly higher than that in the 1st week (P<0.005), and the PCNA expression in the 1st and 2nd week in the experimental group was higher than that in the 1st and 2nd week in the control group (p-value<0.005). In the 1st and 2nd week of tumor implantation, the correlation between IFN-γ and PCNA was negatively correlated(r=－0.566, p-value=0.028; r=－0.604, p-value=0.017), while the correlation between IL-4 and PCNA was positively correlated(r=0.583, p-value=0.023; r=0.884, p-value<0.005). Conclusion: In the VX2 bone tumor model of rabbit, with the extension of time after tumor implantation, there was a negative correlation between IFN-γ and PCNA and a positive correlation between IL-4 and PCNA.

scholarly journals Effect of Iron Supplementation in Anti-Tuberculosis Drug Treatment on Interferon-γ Level in Tuberculosis Spondylitis Patients

2020 ◽  
Vol 52 (3) ◽  
pp. 154-159
Author(s):  
M. F. Nanda Perdana ◽  
Hermawan Nagar Rasyid ◽  
Ahmad Ramdan ◽  
Yoyos Dias Ismiarto
Keyword(s):  
Iron Supplementation ◽  
Defense Mechanism ◽  
Interferon Γ ◽  
Control Group ◽  
P Value ◽  
Major Health ◽  
Ifn Γ ◽  
Randomized Control ◽  
Experimental Group ◽  
Single Blind

Tuberculosis (TB) is still one of the major health problems in the community. Micronutrients, including iron, are crucial in the body's defense mechanism. Iron modulates the activation of IFN-γ, which in turn will activate the macrophages. This study explores the effect of iron supplementation given with anti-tuberculosis drug therapy on the IFN-γ level in tuberculosis spondylitis patients. This was a single-blind randomized control trial study at Dr. Hasan Sadikin General Hospital Bandung, from December 2018 to March 2019, using the consecutive sampling approach. Thirty-four respondents with category 1 TB spondylitis and an sTfR level of> 21.7 pg /µL were recruited and randomized into control and experimental groups. The control group (n=17) was treated with 2 RHZE for 2 months while the experimental group (n=17) received 2 RHZE and iron tablet for 2 months. An increase in the IFN-γ level of the experimental group was observed from an average of 21.7 pg/ml before therapy to an average of 72.39 pg/mL after therapy. Meanwhile, the IFN-γ level in the control group increases from an average of 22.5 pg/mL to an average of 35.35 pg/mL. This difference was statistically significant based on the analysis using the independent t-test (p-value <0.05). Therefore, the administration of anti-tuberculosis drugs with the addition of iron can increase the body's immune response, which is indicated by the increasing level of IFN-γ.

scholarly journals Senam Irama Lagu Gizi Seimbang Meningkatkan Kebugaran Pada Anak Sekolah

2020 ◽  
Vol 5 (1) ◽  
pp. 22-29
Author(s):  
Een Juliasti ◽  
Mury Kuswari ◽  
Idrus Jus’at
Keyword(s):  
Physical Activity ◽  
Elementary School ◽  
Test Group ◽  
Wilcoxon Test ◽  
Control Group ◽  
P Value ◽  
Fitness Level ◽  
Rhythmic Gymnastics ◽  
Post Test ◽  
Experimental Group

Physical activity on students in Jakarta is the lowest if it is compared to various regions in Indonesia. The low physical activity has an impact on the declining of health and fitness so that it affects toward the risk of various non-communicable diseases. The purpose of this research is to know the influence of gymnastics rhythm of Gizi Seimbang’s song to the level of fitness on students in State Elementary School Kebon Jeruk 08 Pagi West Jakarta. The type of research uses design experimental quasi research with design of Pre test - post test group control design. This design involves two groups of subjects, one is given an experimental group and one group is not enforced (control group). The population of this study are children aged 10-12 years old with 60 respondents (30 students of gymnastic group and 30 control group students) with gymnastics 3x/week for 12 times. Data analysis use t-dependent test, wilcoxon test because the data is not normally distributed. The result of the research shows that there is the influence of rhythmic gymnastics of Gizi Seimbang’s song to the significant fitness level (p value = 0.0001). Based on the results of this study, gymnastics rhythm of Gizi Seimbang’s song increases the level of fitness compared with control group on students in State Elementary School Kebon Jeruk 08 Pagi West Jakarta.

scholarly journals Pirzi Doll”: A Method for Increasing Children’s Knowledge and Attitude in Balanced Nutrition

2019 ◽  
Vol 8 (2S9) ◽  
pp. 165-169
Keyword(s):  
Nutrition Education ◽  
Control Group ◽  
P Value ◽  
Nutritional Education ◽  
Control Groups ◽  
School Aged Children ◽  
The Mean ◽  
Knowledge And Attitude ◽  
And Control ◽  
Experimental Group

Nutrition is one of the main problems in the world, where the number of malnourished patients reaches less than 104 million children and one third of all causes of child mortality worldwide are still caused by malnutrition. Nutrition knowledge in school-aged children is one of the factors determining patterns food consumption and nutritional status. Nutrition education in school-aged children can improve children's nutritional knowledge and play a role in food selection and eating habits. Nutrition education should start from an early age. Nutrition and health education began to be directed at kindergarten and elementary school students, since this age group has a habit of attitude that is relatively easy to be formed. This study aims to determine the effect of nutrition education by story’s telling with pirzi doll media to increase knowledge and attitude of children about balanced nutrition. This study used quasi-experimental design with two group pre and post-test design sampling was carried out with a systematic random sampling. Sample of research were children enrolled in Kindergarten IT Rabbani and IT Menara Fitrah in Ogan Ilir District. There were 37 samples on control group dan 39 samples on experimental group. The results of statistical analysis using Mann-Whitney test. The mean score of knowledge after being given a nutritional education was 26.15 ± 3.10 in the experimental group and in the control group was 26.05± 3.16 (p-value=0.817). The mean score of attitudes after being given a nutritional education was 13.69 ± 1.97 in the experimental group and in the control group was 11.24 ± 3.67 (p-value=0.002). There was no difference in mean score of knowledge between the experimental and control groups after being given a nutritional education. There was a difference in mean values of attitudes between the experimental and control groups after being given a nutritional education.

scholarly journals Pengaruh Pendidikan Kesehatan Tentang Inspeksi Asam Asetat (IVA) Terhadap Motivasi Wus dalam Deteksi Kanker Serviks di Puskesmas Putri Ayu Jambi

2019 ◽  
Vol 19 (1) ◽  
pp. 42
Author(s):  
Hesty Hesty ◽  
Rahmah Rahmah ◽  
Nurfitriani Nurfitriani
Keyword(s):  
Cervical Cancer ◽  
Health Education ◽  
Early Stage ◽  
Sampling Technique ◽  
World Health ◽  
Control Group ◽  
P Value ◽  
Childbearing Age ◽  
Control Group Design ◽  
Experimental Group

World Health Organization (WHO), cervical cancer is the number two cause of death in the world for women from all existing cancer. Every two minutes a woman dies of this disease. This cancer attack at an early stage does not cause any symptoms. That is why, why does this type of cancer fall into the category of the silent killer. One of the promotive and preventive efforts in women of childbearing age as primary prevention can be through the provision of health education about cervical cancer using the Acetic Acid Inspection (IVA) method. The purpose of this study is to find out whether there is an influence of health education about IVA on WUS motivation in cervical cancer detection in health centers. Putri Ayu Jambi. This type of quantitative research with non randomized pretest-posttest with control group design, with purposive sampling technique totaling 22 WUS, 11 people as controls and 11 experimental people. The instrument in the form of questionnaires was analyzed by univariate and bivariate Paired Test t-test, which was conducted from April to July 2018.  The results of the study revealed that of the 22 WUSs studied, the average motivation of the pretest in the experimental group was 41.1818 and the control group was 39.9091, while the results of the posttest of the experimental group after being given counseling and leaflets increased by 42.9091 with p-value 0.034. There is an influence of health education about IVA tests on WUS motivation in detecting cervical cancer. It is suggested that the Puskesmas can improve activities in planning disease control programs, especially carrying out counseling and counseling both in the puskesmas building and in the working area of the puskesmas by involving cadres in their environment so that WUS is moved to take the IVA examination.

Effects of Garcinia Cambogia on the Weights of Normal Albino Mice

2021 ◽  
Vol 15 (11) ◽  
pp. 2956-2957
Author(s):  
Gul Afshan ◽  
Ponum Mirani ◽  
Imtiaz Aslam ◽  
Sobia Ibrahim
Keyword(s):  
Control Group ◽  
P Value ◽  
Distilled Water ◽  
Garcinia Cambogia ◽  
Group Iii ◽  
Smart Drugs ◽  
Group I ◽  
Final Weight ◽  
Albino Mice ◽  
Experimental Group

Aim: Effects of available weight reducing drugs on the weights of a normal albino mice. Methodology: In this study, total 39 adult albino mice were used and were divided in three groups containing 13 animals in each. Group I served as control and was given 1 ml of distilled water once a day for 8 weeks. Group II and Group III served as experimental group and mice in these groups were given 0.5 mg of Slim Smart and Ultra Slim Plus drugs dissolved in 1 ml of distilled water respectively once a day for 8 weeks. To support these results weights of kidneys were also measured and relative tissue width index was calculated and compared with control group. Results: The weight of the animals increased in the experimental groups as compared to the control group. The overall difference for final weight among three groups was highly significant with p-value <0.01. When final weight compared group wise, the experimental groups had significantly higher weight as compared to control with p-values <0.01and 0.028 Conclusion: Both Ultra Slim Plus and Slim Smart drugs cause weight to increase in the individuals who are not obese and have BMI in normal range Keywords: Weight loss, Garcinia Cambodia, kidney, Albino mice

scholarly journals KOMBINASI ULTRASOUND DAN DRY NEEDLING LEBIH MENURUNKAN DISABILITAS LEHER DARI PADA KOMBINASI ULTRASOUND DAN HOLD RELAX PADA MYOFASCIAL PAIN SYNDROM OTOT UPPER TRAPEZIUS

2018 ◽  
Author(s):  
Iman Santoso ◽  
Bagus Komang Satriyasa ◽  
Muthiah Munawaroh ◽  
I Nengah Sandi ◽  
Made Muliarta ◽  
...  
Keyword(s):  
Myofascial Pain ◽  
Neck Disability Index ◽  
Control Group ◽  
P Value ◽  
Dry Needling ◽  
Upper Trapezius ◽  
Post Test ◽  
Neck Disability ◽  
Experimental Group ◽  
Better Than

Introduction : Upper trapezius myofascial pain syndrom (MPS) is characterized by presence of trigger points in upper trapezius muscle. MPS can cause disability and also has negative economic effect. Purpose : The reserach’s goal is to improve that combination of ultrasound and dry needling was better than combination of ultrasound and hold relax to decrease neck disability index. Methods : This research used experimental methods to study with pre-test and post-test control group design. Number of samples of the experimental group was 17 subjects given three times treatment of combination of ultrasound and dry needling, while in the control group were 17 subjects given three time treatment of combination of ultrasound and hold relax. NDI was used as out come measure. Shaphiro-wilk test was used to test the normality and levene’s test was used to test the homogenity. wilcoxon signed ranked test was used for hipothesis I, t-test related was used for hipothesis II and t-test independent wa used for hipothesis III. Results : The research showed that: (1) There was significant decrease of NDI in the experimental group . values of mean for pre test were 42,04 + 7,33 % and post test were 10,18 + 3,78 %. with p value = 0.000 (p <0.05) (2) there was significant decrease of NDI in the control group. values of mean for pre test were 45,29 + 6,03 % and post test were 22,24 + 5,42 %, with p value = 0.000 (p <0.05) (3) There were significant differences between experimental group and control group comparing with the differences of post test values between the group. The mean of post test values in experimental group showed 10,18 + 3,78 % meanwhile 22,24 + 5,42% in control group, with p value = 0.000 (p <0.05). Conclution : It was concluded that combination of ultrasound and dry needling and combination of ultrasound and hold relax can decrease the neck disability index. Combination of ultrasound and dry needling was better than combination of ultrasound and hold relax to decrease neck disability index in subject with upper trapezius myofascial pain syndrom.

scholarly journals Early path nursing on neurological function recovery of cerebral infarction

2019 ◽  
Vol 10 (1) ◽  
pp. 160-163
Author(s):  
Ling Chen ◽  
Zhena Han ◽  
Junjie Gu
Keyword(s):  
Cerebral Infarction ◽  
Conventional Treatment ◽  
Neurological Function ◽  
Early Rehabilitation ◽  
Control Group ◽  
P Value ◽  
Treatment Methods ◽  
Rehabilitation Care ◽  
Function Recovery ◽  
Experimental Group

Abstract Purpose to study the application of path type early rehabilitation nursing in the nursing of patients with cerebral infarction and to explore its impact on the recovery of neurological function. Methods Patients with acute cerebral infarction in our hospital were randomly divided into two groups. The control group used conventional treatment methods. The experimental group used path type early rehabilitation care based on conventional treatment methods and observed the curative effect. ResultsThe NIHSS scores in the experimental group were significantly lower than those in the control group, and the P value was less than 0.05, which was statistically significant. Conclusion Path type early rehabilitation nursing has a positive effect on the treatment of patients with cerebral infarction, which contributes to the recovery of neurological function of patients and is worthy of promotion in treatment.

scholarly journals Parental Attachment Patterns in Mothers of Children with Anxiety Disorder

Children ◽  
2020 ◽  
Vol 7 (5) ◽  
pp. 46
Author(s):  
Şaban Karayağız ◽  
Timuçin Aktan ◽  
Lider Zeynep Karayağız
Keyword(s):  
Anxiety Disorder ◽  
Attachment Style ◽  
Parental Attachment ◽  
Pearson Correlation ◽  
Descriptive Analysis ◽  
Study Data ◽  
Control Group ◽  
P Value ◽  
City Hospital ◽  
Experimental Group

Anxiety disorder on of the most common illnesses in the context of psychiatry. Potential causes include genetic and environmental factors, as well as the parental attachment of the individuals. The purpose of this study is to examine the relationship between parental attachment style and anxiety disorders for a group of children and their parents. Study data were collected from the mothers (N = 40) of children with an anxiety disorder who visited a child psychiatry outpatient clinic at a city hospital and a private institution in Kayseri (Turkey) in 2018. For the control group, 40 mothers of children without any mental illness were also included in the study. The purposive sampling method was used in the selection of the participants for both groups (experimental and control). Sociodemographic data sheet and parental bonding instrument (PBI) were utilized as the data collection instruments. Then, data were analyzed based on the descriptive analysis methodology that included mean scores, standard deviation, p-value, t-experimental, two-way ANOVA and Pearson correlation experiments by using SPSS v.22. The findings revealed that the mothers of the participants with a college degree in the experimental group had fewer perceptions of protection (t = 2.38, p < 0.01), but more perception of care from their mothers than fathers (t =−2.28, p < 0.05). In addition, although the perceived care of parents was found lower than the participants in the control group, the participants in both groups evaluated their parents analogously for overprotection. Findings showed that the mothers in the experimental group predominantly described their parents as neglecting.

scholarly journals The Effectiveness Of Mindfulness Training to Improve Fast Boat Crew Self-Control in Bali Indonesia

2020 ◽  
Vol 10 (3) ◽  
Author(s):  
Putu Ugram Swadharma ◽  
Alimatus Sahrah ◽  
Nur Fachmi Budi Setyawan
Keyword(s):  
Mindfulness Training ◽  
Self Control ◽  
Average Score ◽  
Control Group ◽  
P Value ◽  
Not Given ◽  
Average Value ◽  
Collection Data ◽  
Experimental Subjects ◽  
Experimental Group

This research aims to empirically examine the effectiveness of mindfulness training to improve the fast boat ship crews’ self-control in X company Bali, Indonesia. The experimental subjects in this research were 22, 12 as the experimental group and 10 as the control group. Collection data was done by using likert scale of 26 items with reliability of  0.889. The data processed using Mann-Whitney tests, the average value of the experimental group is (16.50) is higher than the average value of the control group (5.5), This means the average score of self-control boat crews receiving treatment is higher than whose not given treatment. The result of reaserch is Mann-Whitney U  = 0,000, Z = -3,974, p (value) or Asymp Sig < 0.05 which means there are statistically significant is differences beetwen two group.  

scholarly journals Effect of Vitamin D on the Expression Level of the IL-10 and IL-4 in Asthmatic Mice

2020 ◽  
Vol 10 (6) ◽  
pp. 7077-7084
Keyword(s):  
Vitamin D ◽  
Inflammatory Cytokine ◽  
Rna Extraction ◽  
Vitamin D Supplementation ◽  
Expression Level ◽  
Control Group ◽  
P Value ◽  
Asthmatic Patients ◽  
Vitamin D Levels ◽  
Anti Inflammatory

Asthma is a chronic disease that affects the airways in the lungs and is common in many countries. Studies have shown that vitamin D levels are associated with the onset and exacerbation of asthma symptoms. Given the role of vitamin D in regulating immune responses and the importance of the two major cytokines IL-4 (an inflammatory cytokine) and IL-10 (an anti-inflammatory cytokine) in immunological processes, in this study, the relationship between vitamin D intake and the level of expression of these ILs in asthmatic mice was investigated. In the case-control study performed in three groups of mice, 10 experimental asthma mice, 10 asthmatic vitamin D-treated mice, and 10 healthy mice as the control group were studied. For analyses, after RNA extraction and cDNA synthesis, the expression level of two IL-4 and IL-10 genes was evaluated by real-time PCR. Finally, the results were analyzed by SPSS software. The results showed that in the control group, the expression level of IL-4 and IL-10 genes was 0.01 and 0.02, respectively. Accordingly, the expression level of the IL-4 gene in asthmatic mice and asthmatic mice treated with vitamin D was 0.6 and 0.2, respectively, while the expression level of the IL-10 in these groups was 1.2 and 2.8, respectively, which showed significant changes (p-value < 0.05) in treated mice compared to the asthmatic mice without treatment. Based on results, in asthmatic mice treated with vitamin D, a significant increase in IL-10 expression was observed (1.2 to 2.8), while IL-4 expression was decreased from 0.6 to 0.2. It appears that vitamin D supplementation in asthmatic patients by affecting T-cell maturation and increasing anti-inflammatory IL-10 and suppressing IL-4 may play a role in reducing the symptoms of asthma.

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