scholarly journals TECHNIQUES IN QUANTIFYING HORMONES IN BIOLOGICAL FLUIDS

2020 ◽  
Vol 8 (9) ◽  
pp. 830-836
Author(s):  
Nilabja Roy Chowdhury ◽  
◽  
Md Habib ◽  
Aakanksha Harit ◽  
◽  
...  
Keyword(s):  
Biological Fluids ◽  
Body Fluids ◽  
Point Of View ◽  
The Body ◽  
Enzyme Linked Immunosorbent Assay ◽  
Proper Treatment ◽  
Sample Collection ◽  
Time Resolved ◽  
Immuno Assay ◽  
Hormonal Concentration

Diagnosing a disease leads to proper treatment of a patient. Various diseases are occurred due to hormonal dis-balances within the body. Minor shift of the hormonal concentration within the body fluids, account for various physio-metabolic disruptions. This leads to formation of many diseases. So, regular or periodic diagnosis of the hormones within body fluids are very important in a clinical point of view. To quantify hormones within the body fluids there are a no. of ways to perform. The procedures may be of radioactive principles or non-radioactive ones. These may be Enzyme Linked Immunosorbent Assay (ELISA), Radio-immuno-assay (RIA), Spectroscopy etc. Modern techniques like Time resolved fluorescence, chemiluminescence etc. are also there. Besides, previously used bioassays are there for experimental usage within the laboratory. The ultimate goal is to elevate the speed of sample collection, faster diagnosis of the hormones and thus, proper treatment of the patient by the physician.

Time-resolved fluoroimmunoassay of aflatoxins.

1989 ◽  
Vol 35 (12) ◽  
pp. 2308-2310 ◽  
Author(s):  
P Degan ◽  
G Montagnoli ◽  
C P Wild
Keyword(s):  
Immunosorbent Assay ◽  
Solid Phase ◽  
Body Fluids ◽  
Enzyme Linked Immunosorbent Assay ◽  
Time Resolved

Abstract Preparation of purified and Eu-labeled antibodies specific for aflatoxins is described. Their use is illustrated by a solid-phase competitive time-resolved fluoroimmunoassay, results of which were correlated with those of an enzyme-linked immunosorbent assay based on use of the unmodified antibody to aflatoxin. This procedure is discussed as a quick, sensitive, and reliable immunoassay for use in mycotoxin screening in foodstuffs and body fluids.

scholarly journals A focus on homocysteine in autism.

2013 ◽  
Vol 60 (2) ◽  
Author(s):  
Joanna Kałużna-Czaplińska ◽  
Ewa Żurawicz ◽  
Monika Michalska ◽  
Jacek Rynkowski
Keyword(s):  
Human Physiology ◽  
Folic Acid ◽  
Dietary Habits ◽  
Biological Fluids ◽  
Body Fluids ◽  
The Body ◽  
Autistic Children ◽  
Pathological Conditions ◽  
Wide Range

Homocysteine is an amino acid, which plays several important roles in human physiology. A wide range of disorders, including neuropsychiatric disorders and autism, are associated with increased homocysteine levels in biological fluids. Various B vitamins: B6 (pyridoxine), B12 (cobalamin), and B9 (folic acid) are required as co-factors by the enzymes involved in homocysteine metabolism. Therefore, monitoring of homocysteine levels in body fluids of autistic children can provide information on genetic and physiological diseases, improper lifestyle (including dietary habits), as well as a variety of pathological conditions. This review presents information on homocysteine metabolism, determination of homocysteine in biological fluids, and shows abnormalities in the levels of homocysteine in the body fluids of autistic children.

Helmont, Glisson, and the Doctrine of the Common Reservoir in the Seventeenth-Century Revolution in Physiology

1983 ◽  
Vol 16 (3) ◽  
pp. 261-272 ◽  
Author(s):  
Jeffrey Boss
Keyword(s):  
Seventeenth Century ◽  
Body Fluids ◽  
Point Of View ◽  
The Body ◽  
Experimental Science ◽  
Unpublished Manuscript ◽  
Fluid Systems ◽  
Irreducible Components ◽  
The Common ◽  
Experimental Findings

The change in the physiological conception of body fluid during the Seventeenth Century exemplifies the beginning of changes in chemical ideas away from humours as irreducible components of fluid systems towards water as the common solvent of an indefinitely large range of solutions. Against the Galenic humoral view of body fluids J.-B. van Helmont (1579–1644) postulated ‘latex’, a humour distributed through the body and common to several body fluids. The theory of latex explained experimental findings and provided a basis for Helmont's introduction of diuretics into the treatment of dropsy. Francis Glisson (1597–1677) adopted the theory of latex. In this paper it is shown that Helmont contradicted, in part at least, the distinctness of humours by a doctrine of a common reservoir from which various body fluids are drawn. It is further argued that, on the available evidence, Helmont is the originator of this idea of a common reservoir. Through hitherto unremarked and unpublished manuscript evidence, it is shown that Glisson, in adopting the theory of latex, and its therapeutic application, modifies and extends it. In the manuscripts Glisson expresses himself in the language of Helmontian philosophy. Given Glisson's known influence (in, for example, spreading Harvey's doctrine of the circulation), the question arises what part, if any, is attributable to him in the transmission of the doctrine of the common reservoir. From the point of view of experimental science, that doctrine makes a break with the past no less radical than does, say, that of the circulation. It appears that we have here yet another major contribution of Helmont to the scientific revolution with, through Glisson, a possible channel of transmission of this contribution.

Determination of p11 multifunctional protein in human body fluids by enzyme-linked immunosorbent assay

2017 ◽  
Vol 41 (S1) ◽  
pp. S423-S423
Author(s):  
H. Jiang ◽  
R. Quan ◽  
Y. Yuan
Keyword(s):  
Cerebrospinal Fluid ◽  
Spinal Anesthesia ◽  
Body Fluids ◽  
The Body ◽  
Enzyme Linked Immunosorbent Assay ◽  
Minimum Detectable Concentration ◽  
Elisa Kit ◽  
Potential Biomarker ◽  
Urine Levels ◽  
Urine Serum

ObjectivesThe diagnosis of major depressive disorder (MDD) is symptom based due to the lack of biological biomarker. p11 protein was recently found to be an important factor mediating depression-like states and antidepressant responses. The aim of the study was to assess whether p11 protein in urine can serve as a potential biomarker for major depression, and the relationship of its levels among urine, serum and cerebrospinal fluid (CSF).MethodsWe obtained urine samples from 13 drug-free MDD patients and 13 age- and gender-matched healthy controls. We also collected urine, serum and cerebrospinal fluid samples from 13 of fracture patients or cesarean section patients in the spinal anesthesia. The concentrations of p11 protein were measured using ELISA.ResultsIn MDD patients, urine levels of p11 protein were all less than the minimum detectable concentration of the ELISA kit. The urine levels of p11 were detectable only in one healthy control. In the spinal anesthesia patients, we can detect p11 concentrations in both serum and urine in only two patients. Besides, levels of p11 were detectable in the serum of one patient and urine of another patient. We were unable to measure CSF levels of p11 in all patients.ConclusionsConcentrations of p11 protein in the body fluids are very low and unstable. The sensitivity of the current p11 ELISA kit is currently unsatisfactory, requiring the development of an ELISA kit of higher sensitivity to determine whether p11 in body fluids can serve as biomarker for depression.Disclosure of interestThe authors have not supplied their declaration of competing interest.

IMPLEMENTATION OF FATWA ULAMA COUNCIL ACEH CONCERNING MAINTAINING FARDHU KIFAYAH FOR THE MUSLIM’S CORPSE INFECTED BY COVID 19 IN BANDA ACEH

2020 ◽  
Author(s):  
Andri Nirwana
Keyword(s):  
Health Workers ◽  
Descriptive Analysis ◽  
Body Fluids ◽  
The Body ◽  
Analysis Approach ◽  
Data Sets ◽  
Infected People ◽  
The Public ◽  
The People ◽  
Banda Aceh

Abstract: The phenomenon of the people who forcibly took covid's corpse 19 from the hospital to be taken care of by Fardhu Kifayah by his family and the community, became a conclusion that there was community doubt about the management of Tajhiz Mayat conducted by the hospital. Coupled with the circulation of the video of the Ruku movement 'in the corpse prayer conducted by unscrupulous parties at the Hospital, became added doubts from the public against the hospital. To solve this problem, this research uses a Descriptive Analysis approach, namely by formulating a question, namely How to arrange Covid 19's body in Banda Aceh and this question will be answered with several theories and data sets from the field. So it was concluded in a conclusion that answered the formulation of the problems mentioned. Theoretically the spread of covid 19 is very fast, the size of the virus is only 0.1 micrometer and is in body fluids, especially nasopharyngeal fluid and oropharyngeal fluids of infected people, fluids in the body of covid 19 bodies can get out through every gap of the body such as mouth, nose, eye and rectum, because it requires special techniques in its management. Fardhu kifayah to covid 19 bodies should be carried out by trained Ustad and trained health workers, so that the spread stopped. The results of this study concluded that the management of the Moslem bodies died at Zainal Abidin Hospital in Banda Aceh was in accordance with the Fatwa of the Aceh Ulama Council (MPU) and the bodies were handled by trained Ustad and health workers.

Integrating Focusing and Differentiation: A Therapeutic Path for Couples in Intimate Relationships

2018 ◽  
Vol 3 ◽  
Author(s):  
Evi Zohar
Keyword(s):  
Couples Therapy ◽  
Therapeutic Potential ◽  
Well Being ◽  
Point Of View ◽  
The Body ◽  
Self Awareness ◽  
Emotional Healing ◽  
Felt Sense ◽  
Inner World

Continuing the workshop I've given in the WPC Paris (2017), this article elaborates my discussion of the way I interlace Focusing with Differentiation Based Couples Therapy (Megged, 2017) under the systemic view, in order to facilitate processes of change and healing in working with intimate couples. This article presents the theory and rationale of integrating Differentiation (Bowen, 1978; Schnarch, 2009; Megged, 2017) and Focusing (Gendlin, 1981) approaches, and its therapeutic potential in couple's therapy. It is written from the point of view of a practicing professional in order to illustrate the experiential nature and dynamics of the suggested therapeutic path. Differentiation is a key to mutuality. It offers a solution to the central struggle of any long term intimate relationship: balancing two basic life forces - the drive for individuality and the drive for togetherness (Schnarch, 2009). Focusing is a body-oriented process of self-awareness and emotional healing, in which one learns to pay attention to the body and the ‘Felt Sense’, in order to unfold the implicit, keep it in motion at the precise pace it needs for carrying the next step forward (Gendlin, 1996). Combining Focusing and Differentiation perspectives can cultivate the kind of relationship where a conflict can be constructively and successfully held in the inner world of each partner, while taking into consideration the others' well-being. This creates the possibility for two people to build a mutual emotional field, open to changes, permeable and resilient.

Presentation

2014 ◽  
Vol 2 (3) ◽  
Author(s):  
Redacción CEIICH
Keyword(s):  
Cultural History ◽  
Social Reality ◽  
Point Of View ◽  
The Body ◽  
Literary Studies ◽  
The Third ◽  
The Social ◽  
Feminist Theories ◽  
History Of

<p class="p1">The third number of <span class="s1"><strong>INTER</strong></span><span class="s2"><strong>disciplina </strong></span>underscores this generic reference of <em>Bodies </em>as an approach to a key issue in the understanding of social reality from a humanistic perspective, and to understand, from the social point of view, the contributions of the research in philosophy of the body, cultural history of the anatomy, as well as the approximations queer, feminist theories and the psychoanalytical, and literary studies.</p>

scholarly journals Transition from Emotional to Rational Advertising for Food Products on the Romanian Market

2017 ◽  
Vol 50 (1) ◽  
pp. 101-108
Author(s):  
A.F. Jităreanu ◽  
Elena Leonte ◽  
A. Chiran ◽  
Benedicta Drobotă
Keyword(s):  
Urban Areas ◽  
Eating Habits ◽  
Well Being ◽  
Point Of View ◽  
The Body ◽  
Positive Effects ◽  
New Methods ◽  
Brand Advertising ◽  
Tangible Evidence ◽  
Health And Well Being

Abstract Advertising helps to establish a set of assumptions that the consumer will bring to all other aspects of their engagement with a given brand. Advertising provides tangible evidence of the financial credibility and competitive presence of an organization. Persuasion is becoming more important in advertising. In marketing, persuasive advertising acts to establish wants/motivations and beliefs/attitudes by helping to formulate a conception of the brand as being one which people like those in the target audience would or should prefer. Considering the changes in lifestyle and eating habits of a significant part of the population in urban areas in Romania, the paper aims to analyse how brands manage to differentiate themselves from competitors, to reposition themselves on the market and influence consumers, meeting their increasingly varied needs. Food brands on the Romanian market are trying, lately, to identify new methods of differentiation and new benefits for their buyers. Given that more and more consumers are becoming increasingly concerned about what they eat and the products’ health effects, brands struggle to highlight the fact that their products offer real benefits for the body. The advertisements have become more diversified and underline the positive effects, from the health and well - being point of view, that those foods offer (no additives and preservatives, use of natural ingredients, various vitamins and minerals or the fact that they are dietary). Advertising messages’ diversification is obvious on the Romanian market, in the context of an increasing concern of the population for the growing level of information of some major consumer segments.

scholarly journals Combined Method to Remove Endotoxins from Protein Nanocages for Drug Delivery Applications: The Case of Human Ferritin

Pharmaceutics ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 229
Author(s):  
Filippo Silva ◽  
Leopoldo Sitia ◽  
Raffaele Allevi ◽  
Arianna Bonizzi ◽  
Marta Sevieri ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Biological Fluids ◽  
Affinity Purification ◽  
Delivery Systems ◽  
Point Of View ◽  
Uniform Structure ◽  
Structure Stability ◽  
Target Cells ◽  
Clinical Phase ◽  
Main Challenge

Protein nanocages represent an emerging candidate among nanoscaled delivery systems. Indeed, they display unique features that proved to be very interesting from the nanotechnological point of view such as uniform structure, stability in biological fluids, suitability for surface modification to insert targeting moieties and loading with different drugs and dyes. However, one of the main concerns regards the production as recombinant proteins in E. coli, which leads to a product with high endotoxin contamination, resulting in nanocage immunogenicity and pyrogenicity. Indeed, a main challenge in the development of protein-based nanoparticles is finding effective procedures to remove endotoxins without affecting protein stability, since every intravenous injectable formulation that should be assessed in preclinical and clinical phase studies should display endotoxins concentration below the admitted limit of 5 EU/kg. Different strategies could be employed to achieve such a result, either by using affinity chromatography or detergents. However, these strategies are not applicable to protein nanocages as such and require implementations. Here we propose a combined protocol to remove bacterial endotoxins from nanocages of human H-ferritin, which is one of the most studied and most promising protein-based drug delivery systems. This protocol couples the affinity purification with the Endotrap HD resin to a treatment with Triton X-114. Exploiting this protocol, we were able to obtain excellent levels of purity maintaining good protein recovery rates, without affecting nanocage interactions with target cells. Indeed, binding assay and confocal microscopy experiments confirm that purified H-ferritin retains its capability to specifically recognize cancer cells. This procedure allowed to obtain injectable formulations, which is preliminary to move to a clinical trial.

scholarly journals Analysis of the ex-vivo transformation of semen, saliva and urine as they dry out using ATR-FTIR spectroscopy and chemometric approach

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Tanurup Das ◽  
Abhimanyu Harshey ◽  
Ankit Srivastava ◽  
Kriti Nigam ◽  
Vijay Kumar Yadav ◽  
...  
Keyword(s):  
Phase I ◽  
Phase Ii ◽  
Ex Vivo ◽  
Body Fluids ◽  
Biochemical Changes ◽  
Partial Least Square ◽  
The Body ◽  
Slow Phase ◽  
Time Since Deposition ◽  
Dry Out

AbstractThe ex-vivo biochemical changes of different body fluids also referred as aging of fluids are potential marker for the estimation of Time since deposition. Infrared spectroscopy has great potential to reveal the biochemical changes in these fluids as previously reported by several researchers. The present study is focused to analyze the spectral changes in the ATR-FTIR spectra of three body fluids, commonly encountered in violent crimes i.e., semen, saliva, and urine as they dry out. The whole analytical timeline is divided into relatively slow phase I due to the major contribution of water and faster Phase II due to significant evaporation of water. Two spectral regions i.e., 3200–3400 cm−1 and 1600–1000 cm−1 are the major contributors to the spectra of these fluids. Several peaks in the spectral region between 1600 and 1000 cm−1 showed highly significant regression equation with a higher coefficient of determination values in Phase II in contrary to the slow passing Phase I. Principal component and Partial Least Square Regression analysis are the two chemometric tool used to estimate the time since deposition of the aforesaid fluids as they dry out. Additionally, this study potentially estimates the time since deposition of an offense from the aging of the body fluids at the early stages after its occurrence as well as works as the precursor for further studies on an extended timeframe.

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