The trematocide activity of Nigella sativa compared with Hapadex against paramphistomum in sheep was investigated. A total number of 30 adult sheep (1-2 years old) were used in this study. The animals were allocated into three equal groups. Hapadex treated group, Nigella sativa extract treated group and Nigella sativa powder treated group. The blood and faecal samples were collected from all animals just before 238 Assiut Vet. Med. J. Vol. 39 No. 78, July 1998. treatment and after 3,7 and

Salmonella genus is widely distributed in nature and causes a spectrum of diseases in man and animals. Salmonella enterica subsp. diarizonae serotype 61: k: 1,5, (7) (SED) is a host adapted to sheep and its presence as saprophytic bacteria in sheep has been described in different countries. Several studies performed in abattoirs reported the presence of SED in healthy sheep in the intestinal content and also in the respiratory tract. In addition, this microorganism has also been isolated from nostril and faecal samples in healthy live animals. For this reason, this microorganism is considered well adapted to sheep, behaving normally these animals as asymptomatic carriers. However, SED has also been reported causing health disorders such as chronic proliferative rhinitis in adult sheep, abortions, testicular lesions in rams or alimentary tract disorders in young animals. The zoonotic potential of this microorganism is also discussed.

Download Full-text

HEPATO-NEPHROPROTECTIVE ACTIVITY OF NIGELLA SATIVA OIL ON PARACETAMOL-INDUCED NEW ZEALAND RABBITS (ORYCTOLAGUS CUNICULUS)

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i11.21854 ◽

2017 ◽

Vol 9 (10) ◽

pp. 225

Author(s):

Nadhira M. Dinar ◽

Sheila Pratiwi ◽

Rain Kihara ◽

Nadia G. Paramita ◽

Nazilla R. Fathurrahman ◽

...

Keyword(s):

Body Weight ◽

New Zealand ◽

Oryctolagus Cuniculus ◽

Nigella Sativa ◽

Treated Group ◽

Nigella Sativa Oil ◽

Oral Group ◽

Nephroprotective Activity ◽

Per Oral ◽

New Zealand Rabbits

Objective: The objective of this work was to investigate the hepato-nephroprotective activity of Nigella sativa (Ranunculaceae) oil on paracetamol-induced New Zealand rabbits (Oryctolagus cuniculus).Methods: Hepato-nephroprotective activity of Nigella sativa oil was demonstrated on six groups of paracetamol-induced New Zealand rabbits (Oryctolagus cuniculus) aged 3-4 mo, three in each group (2 males, 1 female). Group I was normal control (water 1.0 ml/kg of body weight per oral), group II was negative control (water 1.0 ml/kg of body weight per oral), group III was positive control (silymarin 100 mg/kg of body weight per oral), group IV-VI were treated with Nigella sativa oil (NSO) dose of 0.5 mg/kg of body weight, 1.0 mg/kg of body weight, and 2.0 mg/kg of body weight per oral, respectively, for 15 d. At the 16th day, rabbits in group II-VI were induced with paracetamol at a dose of 600 mg/kg of body weight per oral. At the 23rd day the animals were measured for their clinical biochemistry parameters and histological examination.Results: Paracetamol administration dose of 600 mg/kg of BW resulted in the elevation of serum glutamic-oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT), and ureum-N levels of the animals, particularly in group II which was treated only with paracetamol. Normal histology of the liver defines the clear shape of the terminal hepatic venule (THV)/central vein (CV) and sinusoids, whereas that of the kidney defines clear shape of the Bowman capsule and glomerulus shape. Qualitative histological examination of the liver showed that the THV/CV in all groups was normal, however in the paracetamol-treated group, the sinusoids were dilated, necrosis and mass apoptosis were detected. Dilated sinusoids were observed in the silymarin group and in the lower and medium doses of NSO groups. In the highest dose of NSO group the THV/CV and sinusoids were normal, but a local apoptosis and fat degeneration were detected. Qualitative histological examination of the kidney indicated that there was no abnormality of the glomerulus shape, however, mass apoptosis and local necrosis of the kidney were found in the paracetamol-treated group and the silymarin-treated group. The lowest dose of the NSO-treated group showed a normal shape of glomerulus and Bowman capsule, normal apoptosis. No necrosis was observed in the rabbit’s kidney. Higher doses of NSO groups indicated a normal glomerulus shape and Bowman capsule, mass apoptosis and local necrosis.Conclusion: In this study, Nigella sativa oil could maintain the normality of the THV/CV and sinusoids in the liver of paracetamol-induced New Zealand rabbits (Oryctolagus cuniculus). Normal glomerulus shape and Bowman capsule were also confirmed in the kidney of paracetamol-induced animals.

Download Full-text

Antihyperlipidemic Effect of a Polyherbal Mixture in Streptozotocin-Induced Diabetic Rats

Journal of Lipids ◽

10.1155/2013/675759 ◽

2013 ◽

Vol 2013 ◽

pp. 1-6 ◽

Cited By ~ 10

Author(s):

Ahmad Ghorbani ◽

Reza Shafiee-Nick ◽

Hassan Rakhshandeh ◽

Abasalt Borji

Keyword(s):

Blood Glucose ◽

Nigella Sativa ◽

Juglans Regia ◽

Fasting Blood Glucose ◽

Punica Granatum ◽

Treated Group ◽

Diabetic Control ◽

Diabetic Rats ◽

Control Group ◽

Beneficial Effects

The effects of a polyherbal mixture containingAllium sativum,Cinnamomum zeylanicum,Citrullus colocynthis,Juglans regia,Nigella sativa,Olea europaea,Punica granatum,Salvia officinalis,Teucrium polium,Trigonella foenum,Urtica dioica, andVaccinium arctostaphyloswere tested on biochemical parameters in diabetic rats. The animals were randomized into three groups: (1) normal control, (2) diabetic control, and (3) diabetic rats which received diet containing 15% (w/w) of this mixture for 4 weeks. Diabetes was induced by intraperitoneal injection of streptozotocin (55 mg/kg). At the end of experiment, the mixture had no significant effect on serum hepatic enzymes, aspartate aminotransferase, and alanine aminotransferase activities. However, the level of fasting blood glucose, water intake, and urine output in treated group was lower than that in diabetic control rats (P<0.01). Also, the levels of triglyceride and total cholesterol in polyherbal mixture treated rats were significantly lower than those in diabetic control group (P<0.05). Our results demonstrated that this polyherbal mixture has beneficial effects on blood glucose and lipid profile and it has the potential to be used as a dietary supplement for the management of diabetes.

Download Full-text

Evaluation of Tannin Extracts, Leonardite and Tributyrin Supplementation on Diarrhoea Incidence and Gut Microbiota of Weaned Piglets

Animals ◽

10.3390/ani11061693 ◽

2021 ◽

Vol 11 (6) ◽

pp. 1693

Author(s):

Matteo Dell’Anno ◽

Serena Reggi ◽

Valentina Caprarulo ◽

Monika Hejna ◽

Carlo Angelo Sgoifo Rossi ◽

...

Keyword(s):

Gut Microbiota ◽

Metabolic Profile ◽

Control Diet ◽

Treated Group ◽

Weaned Piglets ◽

Group 14 ◽

Large White ◽

Ctrl Group ◽

Faecal Samples ◽

Individual Body

The effects of the dietary administration of a combination of Quebracho and Chestnut tannins, leonardite and tributyrin were evaluated in weaned piglets. A total of 168 weaned piglets (Landrace × Large White) were randomly allotted to two experimental groups (6 pens/group, 14 piglets/pen). Animals were fed a basal control diet (CTRL) and a treatment diet (MIX) supplemented with 0.75% tannin extracts, 0.25% leonardite and 0.20% tributyrin for 28 days. Individual body weight and feed intake were recorded weekly. Diarrhoea incidence was recorded by a faecal scoring scale (0–3; considering diarrhoea ≥ 2). At 0 and 28 days, faecal samples were obtained from four piglets/pen for microbiological and chemical analyses of faecal microbiota, which were then assessed by V3-V4 region amplification sequencing. At 28 days, blood from two piglets/pen was sampled to evaluate the serum metabolic profile. After 28 days, a reduction in diarrhoea incidence was observed in the MIX compared to CTRL group (p < 0.05). In addition, compared to CTRL, MIX showed a higher lactobacilli:coliform ratio and increased Prevotella and Fibrobacter genera presence (p < 0.01). The serum metabolic profile showed a decreased level of low-density lipoproteins in the treated group (p < 0.05). In conclusion, a combination of tannin extract, leonardite and tributyrin could decrease diarrhoea incidence and modulate the gut microbiota.

Download Full-text

Hepatoprotective Effect of Nigella Sativa Linn (Kalajira) On Paracetamol-induced Liver Damage

Bangladesh Medical Journal ◽

10.3329/bmj.v40i3.18677 ◽

2014 ◽

Vol 40 (3) ◽

pp. 52-54

Author(s):

Dipali Rani Pal ◽

Shamusn Nahar ◽

Kanta Roy ◽

Shah Alam Talukder ◽

Md. Motahar Hossain ◽

...

Keyword(s):

Liver Damage ◽

Serum Bilirubin ◽

Serum Alkaline Phosphatase ◽

Nigella Sativa ◽

Treated Group ◽

Protective Role ◽

Serum Alanine Aminotransferase ◽

Nigella Sativa Oil ◽

Serum Aspartate Aminotransferase ◽

Group D

Paracetamol is the widely used non-steroidal anti-inflammatory drugs for the treatment of mild to moderate pain. It causes hepatotoxicity in therapeutic dose for prolonged time. It can induce centrilobular hepatic necrosis in large over doses. Nigella sativa (kalajira) is a medicinal plant has a protective role against hepatotoxicity. Therefore, the present study was designed to observe the protective role of Nigella sativa on paracetamol induced liver damage biochemically in Long Evans rats. The experiment was carried out in the Department of Anatomy, Dhaka Medical college, in the period of July 2003 to June 2004. Sixty matured Long Evans rats of either sex, age of 10-12 weeks and weighing between 150-200 gm were used in this study. They were divided into four equal groups. Group A was vehicle (distilled water) control group, Group B was Nigella oil treated group, Group C was paracetamol treated group and Group D was Paracetamol & Nigella sativa oil treated group. Paracetamol in a single dose of 3gm/kg body weight orally causes hepatotoxicity which was assessed bio-chemically. Nigella sativa oil at a dose of 800mg/kg body weight was administered orally for 4 weeks. It was found that significant elevation of serum alanine aminotransferase, serum aspartate aminotransferase, serum alkaline phosphatase and serum bilirubin level in paracetamol treated group. It was observed that 4 weeks oral treatment of Nigella sativa oil in Group D, decrease the level of serum alanine aminotransferase, serum aspartate aminotransferase, serum alkaline phosphatase and serum bilirubin. The result revealed that Nigella sativa oil able to give protection against paracetamol induced liver damage. However, more sophisticated biochemical studies like glutathione content and malondialdehyde level should be studied further. DOI: http://dx.doi.org/10.3329/bmj.v40i3.18677 Bangladesh Medical Journal 2011 Vol.40(3):52-54

Download Full-text

Efek Protektif Sinergis Kombinasi Minyak Jintan Hitam (Nigella sativa) Komersial dan Madu terhadap Nefrotoksisitas pada Tikus akibat Cisplatin

Jurnal Biotek Medisiana Indonesia ◽

10.22435/jbmi.v9i1.3900 ◽

2020 ◽

Vol 9 (1) ◽

pp. 67-80

Author(s):

Rita Noviana ◽

Muhammad In‟am Ilmiawan ◽

Mitra Handini

Keyword(s):

Combination Index ◽

Nigella Sativa ◽

Thiobarbituric Acid ◽

Treated Group ◽

Male Rats ◽

Thiobarbituric Acid Reactive Substance ◽

Dose Treatment ◽

Treatment Groups ◽

Nigella Sativa Oil ◽

Ad Libitum

Abstrak Cisplatin (CP) merupakan agen kemoterapi yang banyak digunakan dan menyebabkan nefrotoksisitas. Minyak jintan hitam (MJH) dan madu (M) diketahui memiliki efek nefroprotektif. Tujuan: Mengetahui efek dan sinergitas kombinasi MJH dan madu pada kadar malondialdehid (MDA) ginjal tikus yang diberi pajanan CP. Metode: Penelitian ini menggunakan 30 ekor tikus jantan yang dibagi menjadi 10 kelompok: Kontrol Normal (KN) (diet ad libitum), MJH1 (1 mL/kgBB MJH), MJH2 (2 mL/kgBB MJH), M1 (3,7 mL/kgBB M), M2 (7,4 mL/kgBB M), MJH1-M1 (1 mL/kgBB MJH dan 3,7 mL/kgBB M), MJH1-M2 (1 mL/kgBB MJH dan 7,4 mL/kgBB M), MJH2-M1 (2 mL/kgBB MJH dan 3,7 mL/kgBB M), MJH2-M2 (2 mL/kgBB MJH dan 7,4 mL/kgBB M), dan CP (diet ad libitum). MJH dan madu diberikan secara oral selama 21 hari. Pada hari ke-18, KN diberikan NaCl 0,9% 1 ml/kgBB sedangkan sisanya diberikan CP dosis tunggal 8 mg/kgBB secara intraperitoneal. Kadar MDA ginjal diukur menggunakan metode thiobarbituric acid reactive substances (TBARS). Didapatkan kadar MDA ginjal yang lebih rendah pada semua kelompok dosis tunggal dan kombinasi dibandingkan dengan kelompok CP (p=0,000). Semua kelompok dosis kombinasi memiliki indeks kombinasi <1. Kombinasi MJH dan madu memberikan efek protektif sinergis terhadap kerusakan oksidatif jaringan ginjal tikus akibat pajanan CP. Kata kunci: Minyak jintan hitam, madu, indeks kombinasi, cisplatin, nefrotoksisitas Abstract Cisplatin (CP) is a widely used chemotherapeutic agent that induces nephrotoxicity. Nigella sativa oil (NSO) and honey (H) have demonsrated nephroprotective effect. To investigate the effect and synergy of NSO and honey combination on kidney malondialdehyde (MDA) levels in CP-induced rats. Thirty male rats were divided into ten groups: Normal Control (NC), NSO1, NSO2, H1, H2, NSO1-H1, NSO1-H2, NSO2-H1, NSO2-H2, and CP-treated group which received ad libitum diet, 1 mL/kgbwt. NSO, 2 mL/ kgbwt. NSO, 3.7 mL/kgbwt. H, 7.4 mL/kgbwt. H, 1 mL/kgbwt. NSO and 3.7 mL/kgbwt. H, 1 mL/kgbwt. NSO and 7.4 mL/kgbwt. H, 2 mL/kgbwt. NSO and 3.7 mL/kgbwt. H, 2 mL/kgbwt. NSO and 7.4 mL/kgbwt. H, ad libitum diet, respectively, for 21 days. On the 18th day, NC group received 1 ml/kgbwt. NaCl 0.9% while the rest received 8 mg/kgbwt. single dose CP intraperitoneally. Kidney MDA levels was measured using thiobarbituric acid reactive substance (TBARS) method. Kidney MDA levels was significantly lower in all single and combination dose treatment groups compared to CP-treated group (p=0,000). Combination index of all combination dose treatment groups is <1. NSO and honey combination provided synergistic protective effect against CP-induced oxidative damage in kidney tissue in rats. Keywords: Nigella sativa oil, honey, combination index, cisplatin, nephrotoxicity

Download Full-text

Clinicopathological effects of Nigella sativa (kalonji) seeds in Escherichia coli infected Psittaculakrameri.

10.21203/rs.3.rs-157950/v1 ◽

2021 ◽

Author(s):

Muhammad Waqar ◽

Farhan Anwar Khan ◽

Mehboob Ali ◽

Faiz ur Rehman ◽

Muhammad Sohaib Ikram ◽

...

Keyword(s):

Nigella Sativa ◽

Clinical Signs ◽

Feed Consumption ◽

Pack Cell Volume ◽

Mortality And Morbidity ◽

E Coli ◽

Reactive Protein ◽

Faecal Samples ◽

Group B

Abstract The clinicopathological effects of E. coli infected Psittaculakrameri (ring necked parrots) supplemented with N. sativa seeds in feed were evaluated. Faecal samples from “psittacine” species parrots were collected and E. coli was isolated for cultural, morphological, biochemical and in-vitro studies. A total of 27 parrots were selected and divided into 03 groups (A= control, B= no treatment and C= treatment+ infection) having 09 parrots in each and were fed with N. Sativa seeds @100mg/kg from 3rd day. The parrots of group B and C were challenged with 0.1ml pathogenic E. coli @106CFU/ml/bird subcutaneously on day 9th. Clinical signs, mortality and morbidity rates were observed during the trial. For haematology, histopathology and CRP in sera, three birds were slaughtered weekly from each group. Significant decreased in feed consumption, haematological values like red blood cells, haemoglobin and pack cell volume while significant increase in clinical signs, morbidity, mortality, liver, heart, kidneys weights and white blood cell were recorded in group B. C-Reactive protein values was significantly decreased in group C as compared to group B while degenerative changes were seen in liver, heart and kidneys of group B.

Download Full-text

Prevention of Brain Hypoperfusion-Induced Neurodegeneration in Rat’s Hippocampus by Black Cumin Fixed Oil Treatment

IIUM Medical Journal Malaysia ◽

10.31436/imjm.v17i1.309 ◽

2020 ◽

Vol 17 (1) ◽

Author(s):

Marwan Saad Azzubaidi ◽

Imad Matloub Al-Ani ◽

Anil Kumar Saxena ◽

Ghasak Ghazi Faisal

Keyword(s):

Nigella Sativa ◽

Pyramidal Cells ◽

Treated Group ◽

Neuroprotective Activity ◽

Protective Effects ◽

Sham Control ◽

Vessel Occlusion ◽

Black Cumin ◽

Age Related ◽

Hippocampal Pyramidal Cells

Introduction: The oil extract of black cumin seeds Nigella sativa (NSO) demonstrated considerable preservation of spatial cognitive functions in rats subjected to chronic brain hypoperfusion (CBH). The hippocampal CA1 region pyramidal cells are the earliest neurons suffering neurodegeneration following CBH. Objective: The current study was devoted to assess the protective effects of Nigella sativa (NSO) treatment on CA1 hippocampal pyramidal cells of rats subjected to chronic brain hypoperfusion (CBH) that was achieved through permanent two vessel occlusion (2VO) procedure. Methods: Twenty four rats were equally divided into three groups; sham control, untreated 2VO and NSO treated group (2VO with daily oral NSO treatment. After the 10th postoperative week coronal sections of the hippocampus were collected for histopathological and electron microscopical examinations. Results: The number of viable pyramidal cells within CA1 hippocampal region in sham control and NSO treated groups was significantly higher than that of untreated 2VO group, while the difference was not significant when comparing the viable pyramidal cells number of sham control with NSO treated groups. Furthermore, 2VO group showed marked intracellular ultrastructural distortions that were less pronounced in NSO treated group. Conclusion: NSO displayed a robust potential to protect hippocampal pyramidal cells from CBH induced neurodegeneration putting forward its prospective neuroprotective activity against age related cognitive decline of Alzheimer’s disease and vascular dementia.

Download Full-text

Protective Effects of Thymoquinone, an Active Compound of Nigella sativa, on Rats with Benzo(a)pyrene-Induced Lung Injury through Regulation of Oxidative Stress and Inflammation

Molecules ◽

10.3390/molecules26113218 ◽

2021 ◽

Vol 26 (11) ◽

pp. 3218

Author(s):

Mohammad A. Alzohairy ◽

Amjad Ali Khan ◽

Mohammed A. Alsahli ◽

Saleh A. Almatroodi ◽

Arshad Husain Rahmani

Keyword(s):

Oxidative Stress ◽

Lung Injury ◽

Inflammatory Markers ◽

Nigella Sativa ◽

Treated Group ◽

Treated Animal ◽

Therapeutic Effects ◽

Metabolic Complications ◽

Animal Group ◽

Environmental Carcinogen

Benzopyrene [B(a)P] is a well-recognized environmental carcinogen, which promotes oxidative stress, inflammation, and other metabolic complications. In the current study, the therapeutic effects of thymoquinone (TQ) against B(a)P-induced lung injury in experimental rats were examined. B(a)P used at 50 mg/kg b.w. induced lung injury that was investigated via the evaluation of lipid profile, inflammatory markers, nitric oxide (NO), and malondialdehyde (MDA) levels. B(a)P also led to a decrease in superoxide dismutase (SOD) (34.3 vs. 58.5 U/mg protein), glutathione peroxidase (GPx) (42.4 vs. 72.8 U/mg protein), catalase (CAT) (21.2 vs. 30.5 U/mg protein), and total antioxidant capacity compared to normal animals. Treatment with TQ, used at 50 mg/kg b.w., led to a significant reduction in triglycerides (TG) (196.2 vs. 233.7 mg/dL), total cholesterol (TC) (107.2 vs. 129.3 mg/dL), and inflammatory markers and increased the antioxidant enzyme level in comparison with the group that was administered B(a)P only (p < 0.05). B(a)P administration led to the thickening of lung epithelium, increased inflammatory cell infiltration, damaged lung tissue architecture, and led to accumulation of collagen fibres as studied through haematoxylin and eosin (H&E), Sirius red, and Masson’s trichrome staining. Moreover, the recognition of apoptotic nuclei and expression pattern of NF-κB were evaluated through the TUNEL assay and immunohistochemistry, respectively. The histopathological changes were found to be considerably low in the TQ-treated animal group. The TUNEL-positive cells increased significantly in the B(a)P-induced group, whereas the TQ-treated group showed a decreased apoptosis rate. Significantly high cytoplasmic expression of NF-κB in the B(a)P-induced group was seen, and this expression was prominently reduced in the TQ-treated group. Our results suggest that TQ can be used in the protection against benzopyrene-caused lung injury.

Download Full-text

Effect of Nigella sativa and Fenugreek on the Pharmacokinetics and Pharmacodynamics of Amlodipine in Hypertensive Rats

Current Drug Metabolism ◽

10.2174/1389200221666200514121501 ◽

2020 ◽

Vol 21 (4) ◽

pp. 318-325

Author(s):

Mohd Aftab Alam ◽

Yousef Abdullah Bin Jardan ◽

Mohammad Raish ◽

Abdullah Mohammad Al-Mohizea ◽

Abdul Ahad ◽

...

Keyword(s):

Blood Pressure ◽

Nigella Sativa ◽

Treated Group ◽

Pharmacokinetic Parameters ◽

Hypertensive Rats ◽

Remarkable Change ◽

Remarkable Effect ◽

Group I

Background: The present article is related to in-vitro and in-vivo herb-drug interaction studies. Objectives: This study aimed to investigate the effect of Nigella sativa and fenugreek on the pharmacodynamics and pharmacokinetics of amlodipine. Method: Hypertensive rats of group-I were treated with amlodipine and rats of group-II and III were treated with N. sativa, and N. sativa + amlodipine and fenugreek, and fenugreek + amlodipine, respectively. Systolic blood pressure (SBP), diastolic blood pressure (DBP) and mean blood pressure (MBP) of group-I, II and III rats were measured by the “tail-cuff system”. Results: N. sativa, as well as fenugreek, reduced the SBP, DBP and MBP. Simultaneously, administration of fenugreek + amlodipine or N. sativa + amlodipine showed better control of BP. Individually, fenugreek, as well as N. sativa, showed a surprising reduction in the heart rate. There was no remarkable effect of any of these two herbs on Cmax, AUC0-t, Kel, and terminal elimination half-life of amlodipine, but fenugreek altered the Tmax of amlodipine significantly, from 2 ± 1.2h in control to 7.2 ± 1.7h in fenugreek treated group, probably by delaying the absorption. Conclusion: Results of pharmacodynamics and pharmacokinetics studies suggested that simultaneous administration of fenugreek or N. sativa with amlodipine improved the pharmacological response of amlodipine in hypertensive rats, though there was no remarkable change in pharmacokinetic parameters (Cmax, Kel, elimination t1/2, and AUC0-t).

Download Full-text