Apoptosis Caused by Triterpenes and Phytosterols and Antioxidant Activity of an Enriched Flavonoid Extract from Passiflora mucronata

Background: P. mucronata (Pm) comes from South America, Brazil and is characterized as “Maracujá de Restinga”. It is used in folk medicine for its soothing properties and in treating insomnia. Objective: The present study for the first time analyzed the antioxidant and cytotoxicity of the hydroalcoholic leaves extract and fractions from Pm. Method: The cytotoxicity test will be evaluated by different assays (MTT and CV) against human prostate cancer (PC3) and mouse malignant melanoma (B16F10) cell lines, and the antioxidant test by DPPH method. Results: β-Amyrin, oleanolic acid, β-sitosterol and stigmasterol were isolated of the most active, hexane fraction. These substances were tested against the tumor cell lines: β-sitosterol and stigmasterol showed the most relevant activity to PC3 in CV assay and, oleanolic acid to B16F10 by the MTT assay. In addition, it was possible to indicate that the mode of cell death for stigmasterol, presumably is apoptosis. In terms of antioxidant activity, the hydroalcoholic leaves extract presented higher activity (EC50 133.3 µg/mL) compared to the flower (EC50 152.3 µg/mL) and fruit (EC50 207.9 µg/mL) extracts. By the HPLC-MS, it was possible to identify the presence of flavones in the leaf extract (isoschaftoside, schaftoside, isovitexin, vitexin, isoorientin, orientin). Conclusions: P. mucronata hexane fraction showed promising cytotoxic effect against cancer cell lines, and stigmasterol contributes to this activity, inducing apoptosis of these cells. Furthermore, as other Passiflora species, Pm extract showed antioxidant activity and flavones are its major phenolic compounds.

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Chemical characterization and bioactive properties of Geranium molle L.: from the plant to the most active extract and its phytochemicals

Food & Function ◽

10.1039/c5fo01479d ◽

2016 ◽

Vol 7 (5) ◽

pp. 2204-2212 ◽

Cited By ~ 10

Author(s):

V. C. Graça ◽

Lillian Barros ◽

Ricardo C. Calhelha ◽

Maria Inês Dias ◽

Ana Maria Carvalho ◽

...

Keyword(s):

Antioxidant Activity ◽

Cell Lines ◽

Human Cancer ◽

Chemical Characterization ◽

Human Cancer Cell ◽

Human Cancer Cell Lines ◽

Bioactive Properties ◽

First Time ◽

Geranium Molle

The phytochemical characterization, antioxidant activity and in vitro cytotoxicity against human cancer cell lines of Geranium molle L. extracts are reported for the first time.

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Antioxidant Activity of the Sea Grape (Caulerpa Racemasa) as a Lotion

Oriental Journal Of Chemistry ◽

10.13005/ojc/350427 ◽

2019 ◽

Vol 35 (4) ◽

pp. 1443-1447

Author(s):

Aryanti Susilowati ◽

Ardi Eko Mulyawan ◽

Tri Widayati Putri

Keyword(s):

Antioxidant Activity ◽

Quality Standard ◽

Caulerpa Racemosa ◽

Phenol Compounds ◽

Time Production ◽

Antioxidant Content ◽

Dpph Method ◽

Antioxidant Agent ◽

First Time

Caulerpa racemose is one type of seaweed that has the greatest potential in Indonesia. Caulerpa racemose has attracted many attentions as an antioxidant agent which is potential for cosmetic industries. The study aims to analyze the antioxidant content of Caulerpa racemose and determining its effectiveness as an anti-aging lotion. testing the antioxidant activity of Caulerpa racemosa using the DPPH method. Caulerpa racemosa contains phenol compounds that function as antioxidants. The results showed that value antioxidant activity of Caulerpa racemosa lotion is very higher, value IC50 reach 0,58 ppm (a compound is called a very strong antioxidant if the value of IC50 is <50 ppm) and the results test of Caulerpa racemose lotion was appropriate with quality standard SNI 16-4399-1996 about the standards sunscreen preparations for the skin. This paper presents the first-time production lotion from Caulerpa racemose.

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PHYTOCHEMISTRY AND ANTIOXIDANT ACTIVITY OF SOURSOP (ANNONA MURICATA) LEAVES

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2019.v11s6.33524 ◽

2019 ◽

pp. 1-6

Author(s):

FONA QORINA ◽

ADE ARSIANTI ◽

QOTRUNNADA FITHROTUNNISA ◽

NADZILA ANINDYA TEJAPUTRI

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Folk Medicine ◽

Parasitic Infection ◽

Ethanolic Extract ◽

Positive Control ◽

Annona Muricata ◽

Tropical Plant ◽

Ic50 Value ◽

Dpph Method

Objective: Soursop (Annona muricata) is a tropical plant which has been utilized as a folk medicine to treat many diseases including cancer, inflammation and parasitic infection. In this study, we investigated its phytochemistry properties and antioxidant activity against free radicals. Methods: Annona muricata leaves were extracted in three different solvents: ethanol, ethyl acetate and n-hexane. Afterwards, a phytochemistry test and the thin layer chromatography (TLC) method were used to evaluate bioactive compounds contained in the three different extracts. Antioxidant activity from the semi-polar (ethyl acetate) and polar (ethanol) solvents were evaluated by the DPPH (1,1-diphenyl-2-picrylhydrazyl) method, and the antioxidant activity is expressed by IC50 value. The results were compared to ascorbic acid as a positive control. Results: The phytochemistry test showed that the extracts were positive for flavonoids, steroids, alkaloids, glycosides and tannins. Moreover, TLC analysis revealed that there were three chemical compounds contained in the extracts. The results from the DPPH method were that ethanolic extract was shown to have the most potent antioxidant activity with an IC50 value of 35.51 ppm. Conclusion: The ethanolic extract of Annona muricata could be developed as the next promising natural antioxidant source.

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Antioxidant potential study of some synthesized N-heterocycles

Bangladesh Medical Research Council Bulletin ◽

10.3329/bmrcb.v35i2.2564 ◽

2009 ◽

Vol 35 (2) ◽

pp. 49-52 ◽

Cited By ~ 10

Author(s):

Mohammad Mamun Hossain ◽

Sukanta Kumar Shaha ◽

Foysal Aziz

Keyword(s):

Antioxidant Activity ◽

Ascorbic Acid ◽

Anticancer Activity ◽

Heterocyclic Compounds ◽

Antioxidant Properties ◽

Biological Properties ◽

Antioxidant Potential ◽

Heating Method ◽

Dpph Method ◽

First Time

Nitrogen containing heterocyclic compounds such as oxindoles especially isatins and their derivatives have excellent biological properties such as anti-inflammatory, antimicrobial, anticancer activity. In addition, this is first time ever we would like to report the antioxidant properties of the said class of compounds. To determine such an important bioactivity a number of halogenated oxindoles 1-3, their diaza-biphenyl derivatives (4-6), ring extended biphenyl (7) and bis-amide (8) were synthesized by classical heating method. The antioxidant activity of all the synthesized compounds was screened by DPPH method with respect to ascorbic acid. In our present investigation some of the synthesized compounds (1, 2, 3, 5 and 6) were found to be active.Keywords: Antioxidant; Isatin; OxindoleOnline 12 August 2009DOI: 10.3329/bmrcb.v35i2.2564Bangladesh Med Res Counc Bull 2009; 35: 49-52

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Anticancer Activity, Antioxidant Activity, and Phenolic and Flavonoids Content of Wild Tragopogon porrifolius Plant Extracts

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2016/9612490 ◽

2016 ◽

Vol 2016 ◽

pp. 1-7 ◽

Cited By ~ 18

Author(s):

Fuad Al-Rimawi ◽

Suzi Rishmawi ◽

Sharehan H. Ariqat ◽

Mahmoud F. Khalid ◽

Ismail Warad ◽

...

Keyword(s):

Antioxidant Activity ◽

Anticancer Activity ◽

Cell Lines ◽

Plant Extracts ◽

Folk Medicine ◽

Reducing Power ◽

Total Phenolic ◽

Osteosarcoma Cell ◽

Anticancer Activities ◽

Antioxidant Power

Tragopogon porrifolius, commonly referred to as white salsify, is an edible herb used in folk medicine to treat cancer. Samples of Tragopogon porrifolius plant grown wild in Palestine were extracted with different solvents: water, 80% ethanol, and 100% ethanol. The extracts were analyzed for their total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity (AA). Four different antioxidant assays were used to evaluate AA of the extracts: two measures the reducing power of the extracts (ferric reducing antioxidant power (FRAP) and cupric reducing antioxidant power (CUPRAC)), while two other assays measure the scavenging ability of the extracts (2,2-azino-di-(3-ethylbenzothialozine-sulphonic acid (ABTS)) and 2,2-diphenyl-1-picrylhydrazyl (DPPH)). Anticancer activity of the plant extracts were also tested on HOS and KHOS osteosarcoma cell lines. The results revealed that the polarity of the extraction solvent affects the TPC, TFC, and AA. It was found that both TPC and AA are highest for plant extracted with 80% ethanol, followed by water, and finally with 100% ethanol. TFC however was the highest in the following order: 80% ethanol > 100% ethanol > water. The plant extracts showed anticancer activities against KHOS cancer cell lines; they reduced total cell count and induced cell death in a drastic manner.

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Biotinylated xanthohumol: synthesis and in vitro biological evaluation for anticancer therapy

10.20944/preprints201807.0039.v1 ◽

2018 ◽

Author(s):

Monika Stompor ◽

Rafał Podgórski ◽

Marta Świtalska ◽

Joanna Wietrzyk

Keyword(s):

Breast Cancer ◽

Antioxidant Activity ◽

Cancer Cells ◽

Cell Lines ◽

Biological Evaluation ◽

Breast Cancer Cell Lines ◽

Dpph Method ◽

Ic50 Values ◽

Mcf 7

Two biotinylated derivatives of the main hop chalcone xanthohumol (1) were prepared by a one-step synthesis via esterification using biotin and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC HCl) and 4-dimethylaminopyridine (DMAP) as coupling reagents. The products were characterized spectroscopically and their antiproliferative activity toward MCF-7, MCF-10A, HepG2, MDA-MB-231, 4T1 and Balb/3T3 cell lines was investigated using the SRB assay. For all three tested compounds the best activity was noted in the case of human (MCF-7) and mice (4T1) breast cancer cell lines (IC50 values < 9 μM). Both biotinylated derivatives showed higher anticancer activity than xanthohumol (1) towards all types of tested breast cancer cells. Double biotinylated xanthohumol (3) proved to be the most active in inhibiting cell growth, with IC50 values equal to 5.35 ± 1.5 μM for 4T1 and 8.03 ± 0.53 µM for MCF-7 cell lines. Compound 3 was also more active than 1 and 2 against liver cancer cells HepG2 (IC50 = 17.37 ± 5.1 μM), while the IC50 values for 1 and 2 were equal to 21.5 ± 2.7 and 22.1 ± 3.9 µM, respectively. 4‑O‑biotinylxanthohumol (2) was the second most active growth inhibitor, particularly with respect to MCF-7 (IC50 = 6.19 ± 1.7 μM) and 4T1 (IC50 = 6.64 ± 0.4 μM) cell lines. The antioxidant activity was evaluated using the 1.1-diphenyl-2-picrylhydrazyl radical (DPPH) method. All tested compounds (1-3) have antioxidant activity between 2.73 and 3.38 mM. It was reported for the first time that new prenylated chalcones containing the biotin moiety effectively inhibited proliferation of cancer cells in vitro.

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SYNTHESIS, CHARACTERISATION, ANTIOXIDANT AND ANTICANCER EVALUATION OF NOVEL FLAVONE-4-OXIMES

INDIAN DRUGS ◽

10.53879/id.54.11.11170 ◽

2017 ◽

Vol 54 (11) ◽

pp. 7-14

Author(s):

B. S Jayashree ◽

◽

Gurushyam Sri ◽

A Pai

Keyword(s):

Antioxidant Activity ◽

Cell Lines ◽

Scavenging Activity ◽

Hep G2 ◽

Dpph Method ◽

Anti Cancer ◽

Aldehyde Derivative ◽

Cancer Activity ◽

Better Than ◽

Mcf 7

A series of novel flavone-4-oximes were synthesized by the oximation of substituted flavones. The synthesized compounds were characterized by various spectrochemical methods including IR, MS and NMR spectroscopy. Out of the 14 test compounds screened for their antioxidant activity, compounds such as JGS-VI (a N,N dimethyl benzaldehyde derivative) and JGS-VII (a 3,4 dimethoxy benzaldehyde derivative) exhibited antioxidant activity comparable to that of ascorbic acid and quercetin as standards following DPPH method. Compounds such as JGS-II (a p-fluoro benzaldehyde derivative), JGS-IV (a p-methyl benzaldehyde derivative) and JGS-V (a thiophene-2-aldehyde derivative) exhibited antioxidant activity among all the test compounds screened against ABTS. However, none of them showed any significant scavenging activity against nitric oxide scavenging assay in the concentration range of 200 μM-25μM. Further, anti-cancer potency for all the test compounds were evaluated by MTT assay against two different cell lines namely MCF-7 and Hep-G2. Compounds such as JGS-I (a p-chloro benzaldehyde derivative), JGS-II (a p-fluoro benzaldehyde derivative), JGS-IV, JGS-V (a thiophene -2-aldehyde derivative), JGS-VI and JGS-IX (a 3,4 chloro benzaldehyde derivative) exhibited activity better than the rest of the test compounds tested against MCF-7 cell lines. Compounds such as JGS-VI, JGS-VII, JGS-VIII (a p-bromo benzaldehyde derivative) and JGS-IX exhibited anti-cancer activity better than other test compounds tested against Hep-G2 cell lines. Thus, a few of the synthesized test compounds could become promising anti-cancer agents.

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AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAN FRAKSI AKAR TABAR KEDAYAN (Aristolochia foveolata Merr.) DENGAN METODE DPPH (2,2 DIPHENYL-1-PICRILHYDRAZIL)

JFL : Jurnal Farmasi Lampung ◽

10.37090/jfl.v8i1.81 ◽

2019 ◽

pp. 8-14

Author(s):

Risa Supringrum ◽

Siti Jubaidah

Keyword(s):

Antioxidant Activity ◽

Free Radicals ◽

Ethyl Acetate ◽

Ethanol Extract ◽

Ethyl Acetate Fraction ◽

The Body ◽

Hexane Fraction ◽

High Dose ◽

Water Fraction ◽

Dpph Method

Antioxidants are substances that can neutralize free radicals, thus protecting the body from various diseases by binding to free radicals and highly reactive molecules that can damage cells. The use of high-dose synthetic antioxidants is reported to be toxic and carcinogenic. Tabar Kedayan root (Aristolochia foveolata Merr) is one of the native plants from North Kalimantan, which is empirically used as an anti-poison, containing secondary metabolites including alkaloids, tannins, flavonoids. Flavonoids are powerful antioxidants. Some studies report that the function of flavonoids can be to prevent and treat cancer. This study aims to determine the antioxidant activity of ethanol extract and n-hexane fraction, the ethyl acetate fraction of Tabar Kedayan root with 2.2 Diphenyl- 1-Picrilhydrazil (DPPH) method as a free radical compound. The results of the study obtained IC50 values for each sample, at ethyl acetate fraction 267.48 ppm, ethanol extract at 603.80 ppm, ethanol-water fraction 705.43 ppm, n- hexane fraction 1500 ppm. The antioxidant activity tests indicate that ethyl acetate fraction is categorized as weak antioxidant, while the ethanol-water fraction and n-hexane fraction exhibit no antioxidant activity. Keywords : Tabar Kedayan, Antioxidants, 2.2 Diphenyl-1-Picrilhydrazil

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Evaluation of in vitro Antioxidant and Cytotoxic Effect of Methanol Extract from aerial parts of Myristica beddomei King ssp. ustulata W.J. de Wilde

International Journal of Pharmaceutical Sciences and Drug Research ◽

10.25004/ijpsdr.2020.120204 ◽

2020 ◽

pp. 115-121

Author(s):

Neethu Joy ◽

Mahesh Mohan

Keyword(s):

Antioxidant Activity ◽

Anticancer Activity ◽

Cytotoxic Effect ◽

Total Phenolic ◽

Ovarian Cancer Cells ◽

Cytotoxicity Test ◽

Antioxidant Power ◽

Ic50 Value ◽

Dpph Method

Plants are traditionally used for pharmacological activities because of its ability to produce bioactive compounds. Myristica beddomei King ssp. ustulata W.J. de Wilde is an ethnomedicinal plant and it is seen in South Western Ghats of Kerala, India. The present study assessed the phenolic content, flavonoid concentration, in vitro antioxidant and cytotoxic effect of different parts of Myristica beddomei King. The total phenolic contents in the extracts ranged from 96.29 (pericarp) to 314.47 (bark) mg g-1 gallic acid equivalent. The concentration of flavonoids in different plant part extracts ranged from 1.81 to 2.76 mg g-1 equivalent to quercetin. All the parts exhibited potential antioxidant activity with an IC50 value of 2.87 to 9.67 μg ml-1 when compared to the standard ascorbic acid with an IC50 value of 2 μg ml-1 in 1,1-diphenyl-2- picryl-hidrasil (DPPH) method. Bark showed highest activity in terms of DPPH radical scavenging (IC50 value of 2.87 µg ml-1), phosphomolybdenum test (2261.33 ± 1.65 mg g-1 trolox equivalent) and ferric ion reducing antioxidant power (FRAP) (113.1 ± 0.28 µmol Fe2+ µg-1) while pericarp showed low antioxidant activity. The in vitro screening results revealed that the seeds exhibited promising anticancer activity compared to PA1 (Ovarian Cancer) cells (50 % inhibition) were observed at a concentration 100.68 µg ml-1. In cytotoxicity test L929 (Fibroblast) cell line compared to the other parts pericarp, mace and seed needed higher concentration (>240 µg ml-1) for LC50 value. It is a promising plant for further development of antioxidant agent as it got high content of phenolic compounds and potential antioxidant and anticancer activity.

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Phytochemical Screening and Antioxidant Activity of Date(Phoenix Dactylifera) Seed Extracts

International Journal of Recent Technology and Engineering - 2 ◽

10.35940/ijrte.b1018.0782s719 ◽

2019 ◽

Vol 8 (2S7) ◽

pp. 87-89

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Methanol Extract ◽

Ethyl Acetate Fraction ◽

Hexane Fraction ◽

Phytochemical Screening ◽

Water Fraction ◽

Ic50 Value ◽

Dpph Method

Dates consisting of 90% flesh have been known to have various benefits, but the remaining 10% of them in the form of seeds has not been utilized optimally. The date palm contains a fairly high polyphenol compound, which can act as an antioxidant. This study aims to determine the antioxidant activity of methanol extract and fractionation of the extract of dates using the in vitro method of DPPH (1,1-diphenyl-2-picrilhidrazil). The date seeds are made into simplicia, macerated with methanol, and then the extracted methanol is gradually fractionated by n-hexane, ethyl acetate, n-butanol, and water. Each fraction and extract is phytochemically screened using the standard methods and its antioxidant activity is determined by DPPH method. Methanol extract, ethyl acetate, n-butanol, and water fractions contain tannins, flavonoids, alkaloids, and saponin, while the n-hexane fraction not contain phytochemical compound based on the phytochemical screening. The ethyl acetate fraction has the strongest antioxidant activity with the lowest IC50 value, that is equal to 3.72 ± 0.44 μg/mL, followed by methanol extract of 4.71 ± 0.64 μg/mL, n-butanol fraction of 6.62 ± 0.08 μg/mL, water fraction of 53.00 ± 18.96 μg/mL, and n-hexane fraction of 250.19 ± 9.69 μg/ mL, while the IC50 value of vitamin C as the positive control is 4.29 ± 0.74 μg/mL. Therefore, date seeds can be a source of natural antioxidants

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