EFFECTIVENESS AND MECHANISM OF PHYSICIAN HERBS FROM KALI PUTIH BATUR BANJARNEGARA CENTRAL JAVA AGAINST DIABETIC NEPHROPATHY

Objectives: Complications in the kidneys (nephropathy) are one of the chronic complications of diabetes mellitus (DM) most common microvascular and estimated to reach 30–40% of all sufferers of DM. Until now there is no cure drug that can prevent diabetic nephropathy. Therefore, the handling of this issue should be done seriously, one of them through an exploration of drug discovery and drug material. Ristoja in 2015 in the ethnic Javanese Banyumasan successfully explores the types of plants, herb, and traditional medicine culture. One is conducted by the Kaliputih Traditional Medicine, Batur, Banjarnegara, Central Java. Based on the results of the interview, traditional medicine has herb for disease therapy kidney failure which consists of 11 species of plants.Methods: The herbs were extracted by infundation method. Sprague Dawley albino male rats were divided into 3 groups (normal, positive, and negative) and 3 sample test groups with 3 different doses (18, 36, and 54 mL/kg body weight [BW]) previously induced streptozotocin. Observations were carried on the levels blood urea nitrogen (BUN), creatinine, uric acid, and nuclear factor kappa B (NF-κB), cyclooxygenase-2 (COX-2), and transforming growth factor-beta (TGF-β) kidney immunohistochemically and histology analysis.Results: Statistical results showed a significant increase of BUN levels in all dose variation groups after being given herbs, compared to the negative control group. The result of the examination of biochemical parameters of creatinine levels statistic showed significant (p<0.05) decrease in the dose 18 and 36 mL/kg BW compare with the negative group. The result of the study on histopathology kidney organs there are are damages to each test in each organ that is necrosis. The result of NF-κB, COX-2, and TGF-β expression no significant decrease compared with the negative controls.Conclusion: The herbs are not capable of nephropathy diabetic and need more research to know that activity as nephroprotective.

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Efficacy of Isometamidiumin Combination with Verapamil, Chlorpromazine ‎or Sodium-ethylenediaminetetra-acetic Acid in Treatment of Experimental ‎Diminazene Aceturate-resistant Strain of Trypanosoma brucei brucei ‎Infection in Rats

Sahel Journal of Veterinary Sciences ◽

10.54058/saheljvs.v17i4.186 ◽

2020 ◽

Vol 17 (4) ◽

pp. 37-45

Author(s):

I. C. Chukwudi ◽

O. C. Omemgboji ◽

B. M. Anene

Keyword(s):

Acetic Acid ◽

Trypanosoma Brucei ◽

Resistant Strain ◽

Parasite Clearance ◽

Negative Control ◽

Male Rats ◽

Control Group ◽

Sprague Dawley ◽

Trypanosoma Brucei Brucei ◽

Diminazene Aceturate

This study investigated the efficacy of different chemotherapeutic regimes in the treatment of rats experimentally infected with diminazene aceturate-resistant strain Trypanosoma brucei brucei. Thirty Sprague Dawley male rats used for the study were randomly assigned to six groups of five rats eachas follows: group A-uninfected untreated (negative control), group B-infected and untreated (positive control), groups C-F were infected and treated with 1.0 mg/kg isometamidum chloride, administered intramuscularly on day 11 post-infection. However, rats in groups D, E and F received further treatments with 700 mg/kg sodium-ethylenediamine tetra-acetic acid, 0.4 mg/kg verapamil and 3 mg/kg chlorpromazine, respectively, administered orally for four days. Clearance of parasite post-treatment (PT), mortality PT, relapse parasitaemia post-clearance, body weight change, rectal temperature, packed cell volume (PCV), haemoglobin (HB) concentration and red blood cell count (RBC) were determined during the experiment. Result showed parasite clearance PT of 100% in groups D and E, 80% in group F and 20% in group C by 24 hours PT. The infection relapsed on day 35 PT in 40% of rats in group C, on day 37 PT in 20% of rats in group F and lastly 20% of rats in groups D and E on day 39 PT. Rats that received drug combination showed marginal improvement in erythrocytic parameters analysed when compared with those treatment with isometamidium alone. Combination therapy showed faster clearance of parasite from the blood and also prolonged relapse post-clearance, thus had a better promising efficacy when compared to using isometamiduim chloride alone.

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ETHANOLIC EXTRACTS OF HEDYOTIS CORYMBOSA L. IMPROVES MONOSODIUM IODOACETATE-INDUCE OSTEOARTHRITIS IN RAT

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i3.16558 ◽

2017 ◽

Vol 10 (3) ◽

pp. 473

Author(s):

Anton Bahtiar ◽

Fitri Arum Sari ◽

Mega Audina Putri ◽

Natasha Linsie Corona Datunsolang ◽

Ade Arsianti

Keyword(s):

Immune System ◽

Ethanolic Extract ◽

Daily Practice ◽

Negative Control Group ◽

Negative Control ◽

Male Rats ◽

Knee Joints ◽

Control Group ◽

Sprague Dawley ◽

Monosodium Iodoacetate

ABSTRACTObjective: The objective of this study is to explore the effects of the 70% ethanolic extract of pearl grass on the immune system of the osteoarthritismodel rat, characterized by the number of leukocytes and lymphocytes, and the histology of the joint. Osteoarthritis is a degenerative diseasecharacterized by chronic inflammation in the joints. Based on the daily practice of herbal medicine in some community in Indonesia, pearl grassusually used for anti-inflammation but not a lot of data to support it.Methods: We used 36 male rats Sprague-Dawley strain divided into 6 groups. Normal group was given 0.5% of CMC, the negative control group was given0.025 ml of sodium iodoacetate in 0.9% saline, the positive groups control group was given a suspension of glucosamine-chondroitin 135 mg/200 g bb,three were given pearls grass extract in various dose 5.625 mg, 11.25 mg, and 22.5 mg, respectively. 28 days after sodium iodoacetate induction, the extractswere given orally once daily for 21 days. Measurement of inflammation of knee joint and the number of leukocytes and lymphocytes were counted on day14th, 28th, and 49th after sodium iodoacetate induction. After treatment, all rats were sacrified and all knee joints were collected to subject for histology.Results: The results showed that the extract of pearl grass in all doses was able to decrease the number of leukocytes and lymphocytes significantlyand prevent proteoglycan degradation. The results showed that the extract of pearl grass with a given dose variations have antiinflammation effectand been able to protect proteoglycan significantly.Conclusion: Doses 3 (22.5 mg/200 g BW) is the best result. These results indicate that pearl grass can be further investigated as a treatment for osteoarthritis.Keyword: Osteoarthritis, Sodium iodoacetate, Pearl grass, Hedyotis corymbosa L. Lamk., Immune system.

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Renoprotective Effect of Platelet-Rich Plasma on Cisplatin-Induced Nephrotoxicity in Rats

Oxidative Medicine and Cellular Longevity ◽

10.1155/2018/9658230 ◽

2018 ◽

Vol 2018 ◽

pp. 1-10 ◽

Cited By ~ 12

Author(s):

Neveen Salem ◽

Nawal Helmi ◽

Naglaa Assaf

Keyword(s):

Growth Factor ◽

Transforming Growth Factor Beta ◽

Transforming Growth Factor ◽

Platelet Rich Plasma ◽

Male Rats ◽

Intercellular Adhesion Molecule ◽

Control Group ◽

Protective Effects ◽

Intercellular Adhesion Molecule 1 ◽

Histopathological Investigation

Platelet-rich plasma (PRP) has grown as an attractive biologic instrument in regenerative medicine for its powerful healing properties. It is considered as a source of growth factors that may induce tissue repairing and improve fibrosis. This product has proven its efficacy in multiple studies, but its effect on cisplatin-induced nephrotoxicity has not yet been elucidated. The present investigation was performed to estimate the protective impact of platelet-rich plasma against cisplatin- (CP-) evoked nephrotoxicity in male rats. Nephrotoxicity was induced in male Wistar rats by right uninephrectomy followed by CP administration. Uninephrectomized rats were assigned into four groups: (1) control group, (2) PRP group, (3) CP group, and (4) CP + PRP group. PRP was administered by subcapsular renal injection. Renal function, inflammatory cytokines, and growth factor level as well as histopathological investigation were carried out. Treatment with PRP attenuated the severity of CP-induced nephrotoxicity as evidenced by suppressed creatinine, blood urea nitrogen (BUN), and N-acetyl glucosaminidase (NAG) levels. Moreover, PRP depressed intercellular adhesion molecule-1 (ICAM-1), kidney injury molecule-1 (KIM-1), caspase-3, and transforming growth factor-beta 1 (TGF-β1) levels, while enhanced the epidermal growth factor (EGF) level. These biochemical results were reinforced by the histopathological investigation, which revealed restoration of normal renal tissue architectures. These findings highlight evidence for the possible protective effects of PRP in a rat model of CP-induced nephrotoxicity, suggesting a new avenue for using PRP to improve the therapeutic index of cisplatin.

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A Polymeric Diet Rich in Transforming Growth Factor Beta 2 Does Not Reduce Inflammation in Chronic 2,4,6-trinitrobenzene Sulfonic Acid Colitis in Pre-pubertal Rats

10.21203/rs.3.rs-80098/v1 ◽

2020 ◽

Author(s):

Claire Dupont-Lucas ◽

Rachel Marion-Letellier ◽

Mathilde Pala ◽

Charlène Guérin ◽

Asma Amamou ◽

...

Keyword(s):

Transforming Growth Factor Beta ◽

Transforming Growth Factor ◽

Intestinal Inflammation ◽

Growth Failure ◽

Therapeutic Interventions ◽

Male Rats ◽

Trinitrobenzene Sulfonic Acid ◽

Sprague Dawley ◽

Chronic Colitis ◽

Colon Wall

Abstract Background: Pediatric Crohn’s disease is characterized by a higher incidence of complicated phenotypes. Murine models help to better understand the dynamic process of intestinal fibrosis and test therapeutic interventions. Pre-pubertal models are lacking. We aimed to adapt a model of chronic colitis to pre-pubertal rats and test if a polymeric diet rich in TGF-β2 could reduce TNBS-induced intestinal inflammation and fibrosis. Methods: Colitis was induced in 20 five-week-old Sprague-Dawley male rats by weekly rectal injections of increasing doses of TNBS (90 mg/kg, 140 mg/kg and 180 mg/kg) for 3 weeks, while 10 controls received phosphate-buffered saline (PBS). Rats were anesthetized using ketamine and chlorpromazine. After first administration of TNBS, 10 rats were fed exclusively MODULEN IBD® powder, while remaining rats were fed breeding chow. Colitis was assessed one week after last dose of TNBS by histopathology and magnetic resonance colonography (MRC). Results: Histological inflammation and fibrosis scores were higher in TNBS group than controls (p<0.05 for both). MRC showed increased colon wall thickness in TNBS group compared to controls (p<0.01), and increased prevalence of strictures and target sign (p<0.05). Colon expression of COL1A1, CTGF, α-SMA and COX-2 did not differ between TNBS rats and controls. TNBS colitis was not associated with growth failure. Treatment with MODULEN IBD® was associated with growth failure, increased colon weight/length ratio (p<0.01), but did not affect histological scores or MRI characteristics. Colon expression of α-SMA was significantly lower in the MODULEN group vs. controls (p=0.005).Conclusion: Features of chronic colitis were confirmed in this model, based on MRC and histopathology. Treatment with MODULEN did not reverse inflammation or fibrosis.

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The Antidiabetic Effect of Low Doses ofMoringa oleiferaLam. Seeds on Streptozotocin Induced Diabetes and Diabetic Nephropathy in Male Rats

BioMed Research International ◽

10.1155/2015/381040 ◽

2015 ◽

Vol 2015 ◽

pp. 1-13 ◽

Cited By ~ 74

Author(s):

Abdulrahman L. Al-Malki ◽

Haddad A. El Rabey

Keyword(s):

Body Weight ◽

Diabetic Nephropathy ◽

Glycosylated Hemoglobin ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Male Rats ◽

Tissue Homogenate ◽

Control Group ◽

Low Doses

The antidiabetic activity of two low doses ofMoringaseed powder (50 and 100 mg/kg body weight, in the diet) on streptozotocin (STZ) induced diabetes male rats was investigated. Forty rats were divided into four groups. The diabetic positive control (STZ treated) group showed increased lipid peroxide, increased IL-6, and decreased antioxidant enzyme in the serum and kidney tissue homogenate compared with that of the negative control group. Immunoglobulins (IgA, IgG), fasting blood sugar, and glycosylated hemoglobin(HbA1c)were also increased as a result of diabetes in G2 rats. Moreover albumin was decreased, and liver enzymes andα-amylase were not affected. In addition, the renal functions and potassium and sodium levels in G2 were increased as a sign of diabetic nephropathy. Urine analysis showed also glucosuria and increased potassium, sodium, creatinine, uric acid, and albumin levels. Kidney and pancreas tissues showed also pathological alteration compared to the negative control group. Treating the diabetic rats with 50 or 100 mgMoringaseeds powder/kg body weight in G3 and G4, respectively, ameliorated the levels of all these parameters approaching the negative control values and restored the normal histology of both kidney and pancreas compared with that of the diabetic positive control group.

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Thymoquinone-PLGA-PVA Nanoparticles Ameliorate Bleomycin-Induced Pulmonary Fibrosis in Rats via Regulation of Inflammatory Cytokines and iNOS Signaling

Animals ◽

10.3390/ani9110951 ◽

2019 ◽

Vol 9 (11) ◽

pp. 951 ◽

Cited By ~ 1

Author(s):

Sultan A. M. Saghir ◽

Naif A. Al-Gabri ◽

Asmaa F. Khafaga ◽

Nahla H. El-shaer ◽

Khaled A. Alhumaidh ◽

...

Keyword(s):

Pulmonary Fibrosis ◽

Inflammatory Cytokines ◽

Transforming Growth Factor Beta ◽

Water Solubility ◽

Transforming Growth Factor ◽

Male Rats ◽

Control Group ◽

Albino Rats ◽

The Third ◽

Alveolar Septa

Pulmonary fibrosis is considered one of the most chronic interstitial illnesses which are not easily treated. thymoquinone’s (TQ) benefits are still partly problematic due to poor water solubility; therefore, it was loaded onto PLGA-PVA carriers. This study aimed to evaluate the potential effect of TQ-PLGA-PVA nanoparticles (TQ-PLGA-PVA-NPs) on pulmonary fibrosis induced by bleomycin in albino rats. Forty male rats were randomized into four groups. The first group served as the control group; the second and the third groups received bleomycin intratracheally, whereas the third group received TQ-PLGA-PVA-NPs after 4 weeks from bleomycin administration. The fourth group was administrated TQ-PLGA-PVA-NPs alone. The designed nanoparticles appeared around 20 nm size (10–30 nm), had a spherical shape, and had 80% encapsulation efficiency. The histological examination of rats simultaneously treated with TQ-PLGA-PVA-NPs and bleomycin revealed reduction in the thickness of the alveolar septa and improvement of the other lung structures, with the presence of lymphocytes admixed with exfoliated epithelium in a few lumina remaining. Ultrastructural findings revealed marked collagenolysis and the release of nanoparticles from ruptured pneumocytes within the alveolar septa after 14 days from TQ-PLGA-PVA-NPs administration. Very active pneumocyte types II were seen in the TQ-PLGA-PVANP group. Additionally, immunohistochemical expression of inducible nitric oxide (iNOS) and estimation of inflammatory cytokines in lung tissues including interleukin 10 (IL 10) and transforming growth factor-beta (TGF-β1) confirmed the antioxidant and anti-inflammatory effects of TQ-PLGA-PVANPs. The study concluded that TQ-PLGA-PVA-NPs could attenuate the bleomycin-induced pulmonary fibrosis, through the inhibition of lung inflammation and the suppression of bleomycin- induced oxidative stress.

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Wound Healing Effects of Prunus yedoensis Matsumura Bark in Scalded Rats

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2017/7812598 ◽

2017 ◽

Vol 2017 ◽

pp. 1-7 ◽

Cited By ~ 3

Author(s):

Jin-Ho Lee ◽

Kyungjin Lee ◽

Mi-Hwa Lee ◽

Bumjung Kim ◽

Khanita Suman Chinannai ◽

...

Keyword(s):

Wound Healing ◽

Growth Factor ◽

Tissue Regeneration ◽

Transforming Growth Factor Beta ◽

Transforming Growth Factor ◽

Control Group ◽

Sprague Dawley ◽

Tissue Proliferation ◽

Inflammatory Phase ◽

Prunus Yedoensis

Pruni Cortex has been used to treat asthma, measles, cough, urticaria, pruritus, and dermatitis in traditional Korean medicine. The objective of this study was to investigate the effects of Prunus yedoensis Matsumura bark methanol extract (PYE) on scald-induced dorsal skin wounds in rats. Scalds were produced in Sprague-Dawley rats with 100°C water and treated with 5% and 20% PYE (using Vaseline as a base), silver sulfadiazine (SSD), and Vaseline once a day for 21 days, beginning 24 hours after scald by treatment group allocation. The PYE-treated groups showed accelerated healing from 12 days after scald, demonstrated by rapid eschar exfoliation compared to the control and SSD groups. PYE-treated groups showed higher wound contraction rates and better tissue regeneration in comparison with the control group. Serum analysis showed that transforming growth factor beta 1 and vascular endothelial growth factor levels remained high or gradually increased up to day 14 in both PYE groups and then showed a sharp decline by day 21, implying successful completion of the inflammatory phase and initiation of tissue regeneration. These findings suggested that PYE is effective in promoting scald wound healing in the inflammation and tissue proliferation stages.

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Angiotensin blockade attenuates diabetic nephropathy in hypogonadal adult male rats

Canadian Journal of Physiology and Pharmacology ◽

10.1139/cjpp-2018-0572 ◽

2019 ◽

Vol 97 (8) ◽

pp. 708-720

Author(s):

Samy Makary ◽

Mohamed Abdo ◽

Wael Abdo Hassan ◽

Mona K. Tawfik

Keyword(s):

Oxidative Stress ◽

Diabetic Nephropathy ◽

Angiotensin Ii ◽

Transforming Growth Factor Beta ◽

Transforming Growth Factor ◽

Male Rats ◽

Glomerular Hypertrophy ◽

Necrosis Factor Alpha ◽

Angiotensin Blockade ◽

And Oxidative Stress

This study examined the effect of the aromatase inhibitor letrozole (0.5 mg/kg) alone or in combination with the angiotensin-receptor blocker valsartan (30 mg/kg) against streptozocin-induced diabetic nephropathy (DN) in hypogonadal (HG) rats for 12 weeks. First, we tested the HG effect on hormone levels, inflammatory cytokines, and oxidative stress in nondiabetic (ND) and diabetic (D) rats. HG was induced with the luteinizing hormone-releasing hormone antagonist cetrorelix (0.71 mg/kg). Diabetes enhanced hormonal hypogonadism and increased inflammation and oxidative stress. Next, experiments examined the effect of early letrozole and valsartan intervention on DN in HG rats. HG-ND and HG-D rats were treated with letrozole alone or in combination with valsartan. HG-D rats developed proteinuria and had increased blood urea nitrogen and creatinine, and histopathological evidence of renal injury, including glomerular hypertrophy and mesangial expansion. Valsartan alone or in combination with letrozole reduced proteinuria, improved renal functions, and reduced diabetes-induced renal angiotensin II. Both agents ameliorated nuclear factor kappa light chain enhancer of activated B cells, interleukin 1β, interleukin 6, and tumor necrosis factor alpha levels. The combination decreased superoxide dismutase, malondialdehyde, and glutathione peroxidase levels, and prevented glomerular hypertrophy. In HG-D rats, valsartan reduced renal collagen IV and transforming growth factor-beta 1, especially when the testosterone level was corrected by letrozole. Thus, normalizing testosterone and inhibiting renal angiotensin II have a renoprotective effect against DN in HG male rats.

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Antioxidant and Anti Inflammation Effect of Snack Bars from Black Glutinous Rice and Pumpkin Powder

Indonesian Journal of Agricultural Research ◽

10.32734/injar.v2i3.2841 ◽

2019 ◽

Vol 2 (3) ◽

pp. 77-88

Author(s):

Akhmad Mustofa ◽

Nanik Suhartatik ◽

Ega Sulistiyo Ningrum

Keyword(s):

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Male Rats ◽

Control Group ◽

Sprague Dawley ◽

Serum Glutamic Oxaloacetic Transaminase ◽

Natural Medicine ◽

Glutinous Rice ◽

Pumpkin Powder

The study was design to evaluate the antioxidant and anti-inflammatory effect of snack bars that made of black glutinous rice and pumpkin powder and study on the male Sprague Dawley rats. Twenty-five male rats in between the age of 2 and 3 months were selected and randomly divided into 5 groups, which were positive control, negative control, group with sylimarin treatment, snack bars A and snack bars B. Carbon tetrachloride was used to induce hepatic damage. The results showed that group with CCl4 treatment has 3.04 ± 0.16 μmol/L MDA while group with Sylimarin, snack bars A, snack bars B were 0.49 ± 0.05; 0.54 ± 0.05; and 1.15 ± 0.16 μmol/L MDA, respectively. The snack bars had the capacity to decline the damage in liver as much as Sylimarin (standardized natural medicine for hepatotoxicity). The SGPT (Serum Glutamin Pyruvic Transaminase) of the two treatment was 22.24 ± 0.80 U/I for Sylimarin and 22.33 ± 1.03 U/I for snack bars while SGOT (Serum Glutamic Oxaloacetic Transaminase) was 40.78 ± 0.77 U/I for Sylimarin and 40.88 ± 1.25 U/I for snack bars A. Snack bars B has a significantly different value on final SGOT and SGPT.

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Pistacia Terebinthus Coffee Protects against Thioacetamide-Induced Liver Injury in Rats

Acta Medica (Hradec Kralove Czech Republic) ◽

10.14712/18059694.2015.94 ◽

2015 ◽

Vol 58 (2) ◽

pp. 56-61 ◽

Cited By ~ 4

Author(s):

Ibrahim Halil Bahçecıoğlu ◽

Murat Ispiroglu ◽

Mehmet Tuzcu ◽

Cemal Orhan ◽

Mustafa Ulas ◽

...

Keyword(s):

Liver Injury ◽

Transforming Growth Factor Beta ◽

Unsaturated Fatty Acids ◽

Transforming Growth Factor ◽

Tap Water ◽

Control Group ◽

Protective Effects ◽

Sprague Dawley ◽

Necrosis Factor Alpha ◽

Pistacia Terebinthus

Aim/background: Pistacia terebinthus is used as a coffee substitute in the East and Southern Anatolia regions of Turkey. It contains unsaturated fatty acids, tocopherols, polyphenols and carotenoids. P. terebinthus has anti-inflammatory and potential antioxidant activity. In this study we evaluated the protective effects of P. terebinthus coffee (PTC) on thioacetamide (TAA)-induced liver injury in rats. Materials and methods: Twenty-eight male Sprague-Dawley rats were equally randomized into four groups. Chronic liver injury was induced with TAA (100 mg/kg i.p. three times weekly). The first group of rats served as control and received only tap water (G1), and the remaining groups of rats received PTC, p.o (G2); TAA (G3); TAA plus PTC, p.o (G4), respectively. Results: After 8 weeks, PTC intake significantly reduced fibrosis/inflammation scores (p < 0.05) in the livers of TAA-treated group. Compared to control group, PTC intake reduced transforming growth factor beta (TGF-β) concentrations in the liver (p < 0.05). Compared to the TAA group, TGF-β, nuclear factor kappa B (NF)-κB (p < 0.05), tumor necrosis factor alpha (TNF-α) concentrations in the liver tissue were reduced by PTC intake. Discussion and conclusion: PTC intake provided beneficial effects against TAA-induced liver injury in rats. PTC probably suppresses the proinflammatory cytokines through NF-κB signaling pathway.

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