scholarly journals Assessment of antimicrobial activity in Diplostephium phylicoides and Diplostephium revolutum extracts by plates and wells method

2019 ◽  
pp. 219-223
Author(s):  
Janeth Arias Palacios
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Ethyl Acetate ◽  
Penicillium Chrysogenum ◽  
Ethyl Acetate Extract ◽  
Salmonella Typhi ◽  
Inhibitory Effect ◽  
The One

Abstract. In the present work, the evaluation of the antimicrobial activity of Diplostephium phylicoides and Diplostephium revolutum on different microorganisms was carried out on using bacteria such as Escherichia coli (CMPUJ:034), Staphylococcus aureus (CMPUJ:370), Salmonella typhi (CMPUJ:045) and Pseudomonas aeruginosa (CMPUJ:065), yeasts such as Saccharomyces cerevisiae (CMPUJ:H042) and Candida albicans (CMPUJ:H022), and filamentous fungi such as Penicillium chrysogenum (CMPUJ:H061) and Aspergillus niger (CMPUJ:H002). This assessment was made by the method of plates and wells using extracts from the leaves of the previously mentioned plants. The extracts were made with different solvents, ethanol, ethyl acetate, dichloromethane and petroleum ether. The results showed that the ethyl acetate extract of Diplostephium phylicoides has antimicrobial activity against Staphylococcus aureus and Candida albicans; furthermore, the dichloromethane extract showed an inhibitory effect against Saccharomyces cerevisiae. When comparing the extracts of the two plants, under the evaluated conditions, the extracts presented antimicrobial activity, and the ethyl acetate extract of Diplostephium revolutum the one that showed better activity against all the microorganisms.

scholarly journals Phytochemical Screening and in vitro Antimicrobial Activity of Cyperus rotundus Leaf Extracts

2021 ◽  
Vol 4 (1) ◽  
pp. 43-52
Author(s):  
EO Agbo ◽  
JO Ogenyi ◽  
CA Adah ◽  
CT Agber
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Ethyl Acetate ◽  
Plant Extracts ◽  
Methanol Extract ◽  
Cyperus Rotundus ◽  
Diffusion Method ◽  
Methanol Extracts ◽  
Study Results

Cyperus rotundus, a member of family Cyperaceae is a perennial, stoloniferous herb. The study investigated the secondary metabolites present in the leaf of Cyperus rotundus and antimicrobial activities of the hexane, ethyl acetate and methanol extracts. Phytochemical analysis revealed the presence of saponins, alkaloids, phenolic compounds, tannins, steroids, flavonoids, cardiac glycosides and terpenoids. The qualitative test of the extracts show the presence of steroid only in the hexane extract; flavonoid, cardiac glycoside in both the ethyl acetate and methanol extracts; saponin and tannin and phenolics in just the methanol extract. While alkaloid was present in all the extracts, terpenoid was absent in all. Antimicrobial activity of plant extracts were monitored using the agar disk-diffusion method against strains gram-positive bacterial: Staphylococcus aureus, Pseudomonas aeruginosa; and gram-negative bacteria: Escherichia coli, Salmonela typhi, and strains of fungi: Candida albicans and Aspergillus niger. The antimicrobial activity of the extracts was monitored at different concentrations of 50, 25, 12.5 and 6.25 mg/mL prepared from the stock concentration (100mg/mL) by serial dilution; with standard drugs: ciprofloxacin and fluconazole. The inhibition of the test organisms were concentration dependent. The methanol extract showed significantly higher Inhibition Zone (IZ) and Activity Index (AI) against the microbes at all concentrations compared to the ethyl acetate and hexane extracts due to the much phytochemicals (saponins, tanins, phenols, flavonoids, cardiac glycocides and alkaloids) found present in its extract. The overall study results signify the potential of Cyperus rotundus as a source of therapeutic agents. The methanol extract showed the best activity against both bacterial and fungal pathogens at the highest concentration of 100 mg/mL, especially the most susceptible bacteria: Staphylococcus aureus (IZ of 25.0±1.5; AI of 0.96) and Candida albicans (IZ of 25.5±0.5; AI of 0.91). At MIC, MBC and MFC of 6.25 mg/mL and 12.5 mg/mL, the plant extracts showed effective bactericidal and fungicidal effect against the test microbes.

scholarly journals Phytochemical Screening and Antimicrobial Activity from Sonneratia caseolaris Fruit Extract

2019 ◽  
Vol 967 ◽  
pp. 28-33
Author(s):  
Pagarra Halifah ◽  
Hartati ◽  
Rachmawaty ◽  
Hala Yusminah ◽  
A. Rahman Roshanida
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Candida Albicans ◽  
Ethyl Acetate ◽  
Scavenging Activity ◽  
Phytochemical Screening ◽  
Fruit Extract ◽  
Mangrove Plant ◽  
Sonneratia Caseolaris

A study out to investigate the phytochemical screening and antimicrobial activity of Sonneratia caseolaris fruit, a mangrove plant from Maros Regency, South Sulawesi, Indonesia. The Sonneratia caseolaris extract was prepared by two different solvent: ethyl acetate and ethanol 70%. We tested the extract of Sonneratia caseolaris for antioxidant activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH). The phytochemicals were extracted separately with ethyl acetate and ethanol 70% by maceration. The evaluation of antibacterial activity for different solvent with different concentration of Sonneratia caseolaris fruit was measurements of inhibitory power are measured by measuring the diameter of the clear zone formed. In phytochemical test Sonneratia caseolaris fruits extracted by ethyl acetate solvent confirmed the presence of flavanoids, saponin, tannin and phenolic and extracted by ethanol 70% solvent confirmed the presence of alkaloid, saponin and phenolic. The antioxidant activity as indicated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity of the Sonneratia caseolaris fruits extract from the species was found to be quite appreciable. The antioxidant scavenging activity of Sonneratia caseolaris fruit extract by ethyl acetate solvent was 0.38% and by ethanol solvent was 0.08%. Antimicrobial activity of Sonnerati caseolaris fruits ethyl acetate extract obtained significant was from P<0.05 of Escherechia.coli and Candida albicans but not significant of Staphylococcus aureus. The Sonneratia caseolaris extracted by ethanol 70% solvents was highly promising among Escherichia coli, Staphylococcus aureus and Candida albicans with level of significance was from P <0.05. The study suggests Sonneratia caseolaris fruits as a potential source of bioactive compounds with stable antioxidative and antimicrobial properties and can be used as natural antimicrobial/antioxidative agents in clinical, pharmaceutical and food processing industries.

scholarly journals UJI AKTIVITAS ANTIMIKROBA DARI JAMUR LAUT YANG BERASOSIASI DENGAN ORGANISME LAUT SPONS Callyspongia aerizusa

PHARMACON ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 1007
Author(s):  
Fransisca Macpal ◽  
Adithya Yudistira ◽  
Defny S. Wewengkang
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Coral Reef ◽  
Ethyl Acetate ◽  
Marine Fungi ◽  
North Sulawesi ◽  
Liquid Fractionation ◽  
Bioactive Potential

ABSTRACTSponge is the lowest level hollow animal without a spine. Sponges are one of the components of coral reef biota that have bioactive potential that has not been widely used. This study aims to determine whether there is antimicrobial activity of marine fungi associated with sponge Callyspongia aerizusa obtained from Southeast Minahasa Regency, North Sulawesi, against the growth of microbes such as Staphylococcus aureus, Escherichia coli and Candida albicans. Samples were obtained then extracted by maceration using acetone and fractionated using liquid-liquid fractionation with ethyl acetate solvents. The results of this study indicate that the extract from the fungus associated with the Callyspongia aerizusa sponge has activity against Escherichia coli and Candida albicans. Keywords: Antimicrobials, Callyspongia aerizusa, Candida albicans, Escherichia coli, Marine fungi, Staphylococcus aureus.  ABSTRAKSponge (spons laut) adalah hewan berongga tanpa tulang belakang yang paling rendah tingkatannya. Spons merupakan salah satu komponen biota penyusun terumbu karang yang mempunyai potensi bioaktif yang belum banyak dimanfaatkan. Penelitian ini bertujuan untuk mengetahui apakah terdapat aktivitas antimikroba dari jamur laut yang berasosiasi dengan spons Callyspongia aerizusa yang di peroleh dari Kabupaten Minahasa Tenggara, Sulawesi Utara, terhadap pertumbuhan mikroba seperti Staphylococcus aureus, Escherichia coli dan Candida albicans. Sampel diperoleh kemudian diekstraksi secara maserasi dengan aseton dan difraksinasi dengan menggunakan fraksinasi cair-cair menggunakan pelarut etil asetat. Hasil penelitian ini menunjukkan bahwa ekstrak dari jamur yang berasosiasi dengan spons Callyspongia aerizusa memiliki aktivitas terhadap bakteri Escherichia coli dan bakteri Candida albicans. Kata kunci:  Antimikroba, Callyspongia aerizusa, Candida albicans Escherichia  coli,.Jamur laut,  Staphylococcus aureus

scholarly journals UJI AKTIVITAS ANTIMIKROBA EKSTRAK DAN FRAKSI KARANG LUNAK (Sarcophyton sp.) DARI PERAIRAN PULAU BANGKA LIKUPANG TERHADAP PERTUMBUHAN MIKROBA Staphylococcus aureus, Salmonella tyhpimurium, DAN Candida albicans

PHARMACON ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 470
Author(s):  
Mochamad I. Eda ◽  
Defny S. Wewengkang ◽  
Surya Sumantri
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Salmonella Typhimurium ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Chloroform Fraction ◽  
Disc Diffusion ◽  
Bioactive Materials ◽  
Agar Disc

ABSTRACTSarcophyton sp. is able to produce bioactive materials, the bioactive material contained in Sarcophyton sp., is sarcophine. The purpose of this study is to find out whether the extracts from soft coral Sarcophyton sp., in Bangka Island waters Likupang performed antimicrobial activity against several pathogenic microbes, such as Staphylococcus aureus, Salmonella typhimurium, and Candida albicans. Samples were extracted by maceration method with ethanol solvent and the fractionation method used was liquid-liquid.The antimicrobial activity testing method used in this study is the Kirby and Bauer disc diffusion method. The results obtained from the antimicrobial activity test on Staphylococcus aureus, fractions of n-hexane with result 8.27mm and fractions of chloroform 8.41mm. In Candida albicans , the n-hexane fraction has an 8.18mm and the chloroform fraction 8.06. While for Salmonella typhimurium has no activity. The conclusion of this study it was found that only some fractions have antimicrobial activity and categorized as moderate, such as the fractions of n-hexane and chloroform that only have moderate inhibiting activity against Stapphylococcus aureus and Candida albicans while for Salmonella typhimurium, the extracts and fractions of Sarcophyton sp., has no activity to the way to inhibit these bacteria. Keywords: Sarcophyton sp., Antimicrobial, Staphylococcus aureus, Salmonella tyhpimurium, Candida albicans ABSTRAK Sarcophyton sp. mampu menghasilkan bahan bioaktif, bahan bioaktif yang terdapat pada Sarcophyton sp adalah sarcophine. Tujuan dari penelitian ini yaitu untuk mengetahui apakah ekstrak dari Karang Lunak Sarcophyton sp.,di Perairan Pulau Bangka Likupang memiliki aktivitas antimikroba terhadap beberapa mikroba patogen Staphylococcus aureus, Salmonella typhi, dan Candida albicans. Sampel diekstraksi dengan metode maserasi dengan pelarut etanol dan metode fraksinasi yang digunakan yaitu fraksinasi cair – cair. Metode pengujian aktivitas antimikroba yang digunakan dalam penelitian ini yaitu metode difusi agar (disc diffusion Kirby and Bauer). Hasil yang didapat dari uji aktivitas antimikroba pada bakteri Staphylococcus aureus, fraksi n-heksan mendapatkan hasil 8.27mm dan pada fraksi kloroform 8.41mm. Pada jamur Candida albicans, fraksi n-heksan mendapatkan hasil 8.18mm dan pada fraksi kloroform 8.06mm, sedangkan pada bakteri Salmonella typhi tidak memiliki aktivitas. Kesimpulan dari penelitian ini, hanya sebagian fraksi yang memiliki aktivitas untuk penghambat antimikroba dengan dikategorikan sedang, seperti pada fraksi n-heksan dan klorofom pada bakteri Stapphylococcus aureus dan jamur Candida albicans, sementara untuk bakteri Salmonella tyhpi ekstrak dan fraksi sampel Karang Lunak Sarcophyton sp. tidak memiliki aktivitas untuk menghambat bakteri tersebut. Kata Kunci : Sarcophyton sp., antimikroba, Staphylococcus aureus, Salmonella tyhpimurium, Candida albicans

KHẢO SÁT HOẠT TÍNH KHÁNG VI SINH VẬT KIỂM ĐỊNH CỦA LOÀI KÉ HOA ĐÀO (URENA LOBATA L.)

2021 ◽  
Vol 7 (21) ◽  
pp. 183-189
Author(s):  
Thu Hương Trương Thị ◽  
Hoài Vân Trần Thị ◽  
Thùy Dương Trần Thị ◽  
Mai Chu Quỳnh ◽  
Nguyệt Nguyễn Thị
Keyword(s):  
Escherichia Coli ◽  
Saccharomyces Cerevisiae ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Aspergillus Niger ◽  
Ethyl Acetate ◽  
Fusarium Oxysporum

Các cặn chiết ethanol tổng, n-hexane, ethyl acetate và 2 hợp chất 5-hydroxy-6,7-dimetoxyflavanon và 3β-hydroxy-urs-12-en-28-oic phân lập được từ loài Ké hoa đào (Urena lobata) thể hiện hoạt tính kháng tốt đối với 2 chủng vi khuẩn Gram (–) (Escherichia coli và Pseudomonas aeruginosa), 2 chủng Gram (+) (Bacillus subtillis và Staphylococcus aureus), 02 chủng nấm sợi (Aspergillus niger và Fusarium oxysporum), 2 chủng nấm men (Candida albicans và Saccharomyces cerevisiae) với đường kính vòng kháng khuẩn từ 8 đến 28 mm.

scholarly journals Antimicrobial Activity of Allium chinense G. Don.

2017 ◽  
Vol 2 (3) ◽  
pp. 129-138
Author(s):  
Frans Grovy Naibaho ◽  
Maria Bintang ◽  
Fachriyan Hasmi Pasaribu
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Microbial Growth ◽  
Inhibitory Concentration ◽  
Ethyl Acetate Extract ◽  
Salmonella Typhi ◽  
Antimicrobial Activities ◽  
Biologically Active ◽  
E Coli ◽  
Allium Chinense

This study aims to analyze antimicrobial activity of Allium chinense G. Don extract against Eschericia coli, Salmonella typhi, Staphylococcus aureus, Bacillus subtilis and Candida albicans, and to examine the active compounds. Allium chinense G. Don was extracted using maseration method and treated with ethanol 70% (v/v), ethanol 96% (v/v), ethyl acetate, n-hexane, and aquadest. Antimicrobial activity assay was conducted using agar difusion method and compounds analysis using Gas chromatography-mass­spectrometry (GC-MS). Antimicrobial activities assay showed that all extracts could inhibit microbial growth. Ethyl acetate extract has the highest antimicrobial activity against all the microbial test. Minimum inhibitory concentration (MIC) of ethyl acetate extract against C. albicans, B. subtilis, E. coli, S. aureus, S. typhi was 25, 100, 250, 250, 1000 mg ml-1 respectively. As many as 25 compounds were derived from GC-MS analysis and most of them were known as the antimicrobial compounds. This study revealed that Allium chinense G. Don contains biologically active compunds as antimicrobial agent particularly anti Candida.

scholarly journals Antimicrobial activity from endophytic fungi Arthrinium state of Apiospora montagnei Sacc. and Papulaspora immersa

2010 ◽  
Vol 53 (3) ◽  
pp. 629-632 ◽  
Author(s):  
Henrique Pereira Ramos ◽  
Glaucia Hollander Braun ◽  
Mônica Tallarico Pupo ◽  
Suraia Said
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Endophytic Fungi ◽  
Ethyl Acetate Extract ◽  
Smallanthus Sonchifolius ◽  
Crude Extracts ◽  
First Time ◽  
Kocuria Rhizophila

Papulaspora immersa and Arthrinium state of Apiospora montagnei Sacc. were isolated from the roots of Smallanthus sonchifolius (yacón). The crude extracts from their cultures inhibited the growth of Staphylococcus aureus, Kocuria rhizophila, Pseudomonas aeruginosa and Escherichia coli. The more relevant results were observed in the ethyl acetate extract from P immersa against P aeruginosa (90 µg/mL) and ethyl acetate extract from Arthrinium state of A montagnei Sacc. against P aeruginosa (160 µg/mL). The two endophytic fungi isolated from yacón roots as well as their antimicrobial activity detected in the crude extracts cultures were being reported for the first time.

Evaluation of the Antimicrobial Activity of Saponins Extract of Trianthema portulacastrum

2009 ◽  
Vol 2 (3) ◽  
pp. 667-670
Author(s):  
Gani Sharmila Banu ◽  
Ganesan Kumar ◽  
Palaniappan Umamagesh ◽  
Subramanian Karthikeyan
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Inhibitory Effect ◽  
Bacterial Activity ◽  
Gram Positive ◽  
Gram Negative ◽  
Trianthema Portulacastrum ◽  
Pathogenic Microbes

The saponin extract of Trianthema portulacastrum were screened for anti-bacterial activity against three pathogenic microbes; Escherichia coli, Staphylococcus aureus and Candida albicans. The extract inhibited the growth of the S. aureus. It was concluded that the saponins have inhibitory effect on gram-positive organism but not on gram negative organism and the fungi.

scholarly journals ANTIMICROBIAL ACTIVITY OF HYMENAEA COURBARIL L. FRUIT

2020 ◽  
pp. 200-203
Author(s):  
MARIANA NARVAEZ CORREA ◽  
OSCAR EDUARDO RODRÍGUEZ AGUIRRE ◽  
JANETH DEL CARMEN ARIAS PALACIOS
Keyword(s):  
Escherichia Coli ◽  
Saccharomyces Cerevisiae ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Candida Albicans ◽  
Aspergillus Niger ◽  
Hymenaea Courbaril ◽  
Test Conditions

Objective: The assessment of the antimicrobial activity of Hymenaea courbaril L. on different microorganisms was realized with four bacteria, Escherichia coli, Staphylococcus aureus, Bacillus subtilis, and Pseudomonas aeruginosa, two yeasts Candida albicans and Saccharomyces cerevisiae, and finally two filamentous fungi Aspergillus niger. Methods: The method of plates and wells was used, using extracts from the fruit of the plant mentioned above. These extracts were made with different solvents such as hexane, dichloromethane, ethanol, and aqueous. Results: It was determined that the dichloromethane extract of H. courbaril L. has antimicrobial activity against the bacterium S. aureus showing a percentage of inhibition of 1.47%. Conclusions: In comparison to bacteria, fungi do not represent a significant inhibitory capacity which represents that when comparing these extracts of this plant, under the test conditions evaluated, it was presented that they obtained antimicrobial activity against S. aureus.

scholarly journals Antimicrobial and in vitro cytotoxic studies of Acampe praemorsa and Aeridis odarata of Orchidaceae

2018 ◽  
Vol 7 (2) ◽  
pp. 2088
Author(s):  
Jhansi Katta ◽  
Khasim S.M.
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Eastern Ghats ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Mcf 7 ◽  
Ethyl Acetate Extracts

This paper aims at studying antimicrobial efficacy and in vitro cytoxic activity of epiphytic orchids namely Acampe praemorsa, Aeridis odorata distributed in Eastern Ghats of Visakhapatnam district. Plants were collected, identified following literature, shade dried and Methanol, ethyl acetate extracts were prepared for the systematic investigation of antimicrobial activity of plant extracts. Antibacterial activity against three gram positive bacteria Bacillus megaterium, Lactobacillus acidophillus and Enterococcus faecalis, three gram negative bacteria Proteus vulgaris, Klebsiella pneumoniae and Escherichia coli was done using Agar well diffusion method, antifungal activity was carried against Candida albicans, Aspergillus flavus by Cpzapak dox agar media, MIC by broth dilution method, zones of inhibition were recorded. Ethyl acetate extracts showed maximum antimicrobial activity against all bacteria and fungi, Aeridis odarata ethyl acetate extract showed highest zone of inhibition 17mm against Lactobacillus acidophilus in bacteria, Ethyl acetate extract of Acampe praemorsa showed highest zone of inhibition 17mm against Candida albicans in fungi, the leaf extracts were tested for its inhibitory effect on HeLa and MCF-7 cell lines were evaluated by the MTT assay and methanolic extract of Aeridis odarata has significant cytotoxicity effect on MCF-7 cell line in concentration range between 5 to 100µg/ml, with IC 50 (µg/ml) value is 26.2. These plants have good antimicrobial activity, further investigation on the phytochemistry of bioactive compounds of these plants would result in discovery of new drugs and further pharmacological investigation of anti-cancer activity of Aeridis odarata should be done.

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