scholarly journals Toxic Effect Of Cypermethrin On Enzymes In Freshwater Fish Oreochromis Mossambicus (Tilapia)

2021 ◽  
Vol 11 (6) ◽  
pp. 137-143
Author(s):  
. Kalpana Devi K. K ◽  
Prakash Sahaya leon J.
Keyword(s):  
Toxic Effect ◽  
Freshwater Fish ◽  
Aquatic Organisms ◽  
Oreochromis Mossambicus ◽  
Control Group ◽  
Synthetic Pyrethroids ◽  
Tissue Samples ◽  
Lethal Level ◽  
Liver And Kidney ◽  
Cellular Necrosis

In agriculture, excessive use of various pesticides had increased in developing countries. These pesticides may be highly toxic, not only to aquatic organisms like fishes but also to humans. In recent years, synthetic pyrethroids were developed for major uses in various agriculture practices and public health purposes. We aim to find the toxic Effect of Cypermethrin on Freshwater Fish Oreochromis mossambicus (Tilapia). The fish Oreochromis mossambicus were exposed for 30 days to various sub lethal concentrations (1/10, 1/20, and 1/30) of cypermethrin. After completion of a 30 days exposure, the fish Oreochromis mossambicus was sacrificed and tissue samples of muscle, liver, and kidney were analyzed. Decreased value of ALP, ACP, and Increased value of AST, ALT was observed in all the sub lethal exposure of cypermethrin on treated freshwater fish Oreochromis mossambicus at 30 days on comparing with the control group. High variation of AST and ALT were observed in the liver at 1/10th concentration of cypermethrin and elevated variation of ALP and ACP was observed in the liver at 1/10th concentration of sub lethal level for 30 days exposure. While comparing with the control group, the effects of cypermethrin on experimental fish showed increased levels of AST, ALT in the tissues of muscle, liver, and kidney of O. mossambicus, and the level of ACP and ALP were found in a decreased manner. Increased levels of ALT and AST activity observed in freshwater fishes may be due to the reduction of metabolic activity and concentration of ACP and ALP in various tissues due to cellular necrosis. In the present study, we found that the effect of cypermethrin alters the activity of various enzymes in freshwater fish Oreochromis mossambicus. It is concluded from this study that exposure to cypermethrin affects the enzyme activities of fish.

scholarly journals Hepatorenal protective effect of ocimum sanctum in chickens toxicated by gentamicin

2021 ◽  
Vol 9 (1) ◽  
pp. 50
Author(s):  
Hadeer A. M. Abdul Ghaffar ◽  
Ashraf A. A.Elkomy ◽  
Enas A.H. Farag
Keyword(s):  
Protective Effect ◽  
Toxic Effect ◽  
Aqueous Extract ◽  
Treated Group ◽  
Ocimum Sanctum ◽  
Control Group ◽  
Broiler Chicks ◽  
Tissue Samples ◽  
Liver And Kidney ◽  
Histopathological Lesion

Background: ocimum sanctum (Tulsi) “Queen of herbs” is considered as sacred and medicinal plant in a lot of countries around the world especially India. Therefore this study was carried out to investigate the heptorenal protective effect of ocimum sanctum aqueous extract against gentamicin induced hepatorenal toxicity in chickens.Materials and Methods: A total 100, one-day old unsexed broiler chicks of a commercial breed (Cobb breed) were used in this study, the chickens were subjected to different treatments blood and tissue samples were collected at 5th week of age post administration, biochemical and histopathological examinations were utilized to investigate protective effect of ocimum sanctum aqueous extract on liver and kidney.Results: gentamicin recorded significant (P<0.05)increase in serum level of aspartate aminotransferase (AST), alanine aminotransferase (ALT), uric acid and creatinine levels when compared with control group, the administration of ocimum sanctum aqueous extract with gentamicin ameliorate their toxic effect, the group treated with ocimum sanctum aqueous extract only similar to control, while gentamicin induce significant (P<0.05)decrease in serum albumin, total protein and globulin levels when compared with control group, ocimum sanctum aqueous extract with gentamicin restore their toxic effect. The group treated with ocimum sanctum aqueous extract only as control. Gentamicin induce histopathological alterations in liver and kidney tissues by comparison with control group and ocimum sanctum aqueous extract treated group as control, while the administration of ocimum sanctum aqueous extract with gentamicin showing improvement in histopathological lesion compared with toxic effect induced by gentamicin.Conclusions: The hepatotoxicity and nephrotoxicity induced by gentamicin were ameliorated by aqueous extract of ocimum sanctum especially at dose of 2ml/liter in drinking water for 12 days.   

scholarly journals The Effectiveness of Dietary Byproduct Antioxidants on Induced CYP Genes Expression and Histological Alteration in Piglets Liver and Kidney Fed with Aflatoxin B1 and Ochratoxin A

Toxins ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 148
Author(s):  
Roua Gabriela Popescu ◽  
Cristina Bulgaru ◽  
Arabela Untea ◽  
Mihaela Vlassa ◽  
Miuta Filip ◽  
...  
Keyword(s):  
Gene Expression ◽  
Ochratoxin A ◽  
Aflatoxin B1 ◽  
Basal Diet ◽  
Sea Buckthorn ◽  
Expression Level ◽  
Control Group ◽  
Tissue Samples ◽  
Perform Gene Expression ◽  
Liver And Kidney

The purpose of this study was to investigate the potential of a byproduct mixture derived from grapeseed and sea buckthorn oil industry to mitigate the harmful damage produced by ochratoxin A and aflatoxin B1 at hepatic and renal level in piglets after weaning. Forty cross-bred TOPIGS-40 hybrid piglets after weaning were assigned to three experimental groups (E1, E2, E3) and one control group (C), and fed with experimental diets for 30 days. The basal diet was served as a control and contained normal compound feed for starter piglets without mycotoxins. The experimental groups were fed as follows: E1—basal diet plus a mixture (1:1) of two byproducts (grapeseed and sea buckthorn meal); E2—the basal diet experimentally contaminated with mycotoxins (479 ppb OTA and 62ppb AFB1); and E3—basal diet containing 5% of the mixture (1:1) of grapeseed and sea buckthorn meal and contaminated with the mix of OTA and AFB1. After 4 weeks, the animals were slaughtered, and tissue samples were taken from liver and kidney in order to perform gene expression and histological analysis. The gene expression analysis showed that when weaned piglets were fed with contaminated diet, the expression of most analyzed genes was downregulated. Among the CYP450 family, CYP1A2 was the gene with the highest downregulation. According to these results, in liver, we found that mycotoxins induced histomorphological alterations in liver and kidney and had an effect on the expression level of CYP1A2, CYP2A19, CYP2E1, and CYP3A29, but we did not detect important changes in the expression level of CY4A24, MRP2 and GSTA1 genes.

scholarly journals Liquorice (glycyrrhiza glabra) hydroethanolic extracts effectively alleviate thioacetamide -induced hepatotoxicity and hematotoxicity in experimental rats through their antioxidant nature

2020 ◽  
Vol 9 (1) ◽  
pp. 10
Author(s):  
Alaa Behairy ◽  
Ashraf Elkomy ◽  
Faten Elsayed
Keyword(s):  
Toxic Effect ◽  
Radical Scavenging Activity ◽  
Economic Value ◽  
Radical Scavenging ◽  
Treated Group ◽  
Glycyrrhiza Glabra ◽  
Male Rats ◽  
Control Group ◽  
Tissue Samples ◽  
Hydroethanolic Extract

Background: Liquorice (Glycyrrhiza glabra) is a high valued plant and used in a lot of countries around the world. The liquorice (LQ) has a remarkable medicinal, nutritional and socio-economic value. Therefore this study was designed to clarify the protective effect of liquorice hydroethanolic extract against Thioacetamide (TAA) induced hepatotoxicity and hematotoxicity in rats.Materials and Methods: Seventy white Albino male rats were used in this study and after acclimatization rats were subjected to different treatments blood and tissue samples were collected after day 30 post administration, biochemical, antioxidant, hematological and histopathological examinations were utilized to investigate hepatoprotective activity of liquorice hydroethanolic extract.Results: TAA significant (P<0.05) increase the activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and hepatic malondialdehyde (MDA) compared to control group and silymarin(SL)treated group as stander, admin-istration of LQ extract restore the toxic effect of TAA , while TAA significant (P<0.05) decrease the levels of catalase(CAT) ,hepatic glutathione(GSH) hepatic superoxide dismutase (SOD) , total protein and albumin level compared to control group and silymarin(SL)treated group as stander, LQ extract ameliorate toxic effect of TAA. TAA induce hematotoxicity in rats by significant (P<0.05) decrease Hb content and RBCs, whereas WBCs count significantly (P<0.05) increased throughout the period of administration when compared to the rats in control group and silymarin(SL)treated group as stander,LQ administration protect against the hematotoxicity effect of TAA, TAA induce histopathological alteration in liver tissue by comparison with control group and silymarin(SL)treated group as stander, while LQ extract showing improvement in the histopathological lesion compared with toxic effect induced by TAA.Conclusions: The hepatotoxicity and hematotoxicity induced by TAA were ameliorated by hydroethanolic extract of LQ especially in double dose (200 mg/kg b.wt). This effect was attributed to free radical scavenging activity and potent antioxidant activity of its components (flavonoid, tannin and saponin). 

Tissue metabolic changes for effects of pirfenidone in rats of acute paraquat poisoning by GC-MS

2017 ◽  
Vol 33 (12) ◽  
pp. 887-900 ◽  
Author(s):  
Jianshe Ma ◽  
Fa Sun ◽  
Bingbao Chen ◽  
Xiaoting Tu ◽  
Xiufa Peng ◽  
...  
Keyword(s):  
Treatment Group ◽  
Gas Chromatography Mass Spectrometry ◽  
Control Group ◽  
Rat Tissues ◽  
Tissue Samples ◽  
Metabolic Alterations ◽  
Treatment Groups ◽  
Liver And Kidney ◽  
Rat Tissue ◽  
And Control

We developed a metabolomic method to evaluate the effect of pirfenidone on rats with acute paraquat (PQ) poisoning, through the analysis of various tissues (lung, liver, kidney, and heart), by gas chromatography–mass spectrometry (GC-MS). Thirty-eight rats were randomly divided into a control group, an acute PQ (20 mg kg−1) poisoning group, a pirfenidone (20 mg kg−1) treatment group, and a pirfenidone (40 mg kg−1) treatment group. Partial least squares-discriminate analysis (PLS-DA) revealed metabolic alterations in rat tissue samples from the two pirfenidone treatment groups after acute PQ poisoning. The PLS-DA 3D score chart showed that the rats in the acute PQ poisoning group were clearly distinguished from the rats in the control group. Also, the two pirfenidone treatment groups were distinguished from the acute PQ poisoning group and control group. Additionally, the pirfenidone (40 mg kg−1) treatment group was separated farther than the pirfenidone (20 mg kg−1) treatment group from the acute PQ poisoning group. Evaluation of the pathological changes in the rat tissues revealed that treatment with pirfenidone appeared to decrease pulmonary fibrosis in the acute PQ poisoning rats. The results indicate that pirfenidone induced beneficial metabolic alterations in the tissues of rats with acute PQ poisoning. Rats with acute PQ poisoning exhibited a certain reduction in biochemical indicators after treatment with pirfenidone, indicating that pirfenidone could protect liver and kidney function. Accordingly, the developed metabolomic approach proved to be useful to elucidate the effect of pirfenidone in rats of acute PQ poisoning.

Effects of Monocrotophos on Some Biochemical Parameters in Fresh Water Fish Oreochromis Mossambicus (Tilapia)

GIS Business ◽  
2019 ◽  
Vol 14 (6) ◽  
pp. 224-229
Author(s):  
A. Solaiappan ◽  
J. Prakash Sahaya Leon
Keyword(s):  
Amino Acid ◽  
Biochemical Parameters ◽  
Amino Acid Level ◽  
Exposure Period ◽  
Oreochromis Mossambicus ◽  
Sublethal Concentration ◽  
Control Group ◽  
Fresh Water Fish ◽  
Tissue Samples ◽  
Water Fish

The toxic effect of Monocrotophos on some biochemical parameters such as Protein and Amino acid in freshwater fish Oreochromis mossambicus were studied. The fishes exposed to 1/10th, 1/20th, and 1/30th sublethal concentration of Monocrotophos for 30 days. The tissue samples of gill, liver were analysed at the end of the 30th day exposure period. A decreased value of Protein and Amino acid were observed in 1/10th, 1/20th, and 1/30th sub lethal concentration of Monocrotophos at the exposure period of 30 days while comparing with the control group. The high variation observed in the 1/10th sublethal level of 30thday exposure. While compare with control group the effects of Monocrotophos on fish found during this experiment showed that the level of protein and amino acid level in various tissues (gill and liver) of Oreochromis mossambicus decreased.

scholarly journals EFFECTS OF FEEDING GENETICALLY MODIFIED (GM) MAIZE ON OXIDATIVE STRESS PARAMETERS IN NEW ZEALAND RABBIT

2018 ◽  
Vol 20 (1) ◽  
pp. 173-176 ◽  
Keyword(s):  
New Zealand ◽  
Genetically Modified ◽  
Kidney Tissue ◽  
Experimental Period ◽  
Control Group ◽  
Tissue Samples ◽  
Treatment Groups ◽  
Gm Maize ◽  
Liver And Kidney ◽  
New Zealand Rabbits

In this study, we investigated the effects of Genetically Modified (GM) Maize on liver and kidney tissue weights, malondialdehyde (MDA) and glutathione (GSH) levels of liver and kidney tissues in New Zealand Rabbits. For this purpose, rabbits were divided into three groups: parental, first-generation and second-generation. Each group was divided into two sub-groups i.e. control group and the treatment group. Rabbits on the control group were fed with conventional maize (non-GM), whereas rabbits which are in the treatment groups were fed with GM maize. Six females and six male New Zealand rabbits were used per group. At the end of the experimental period, tissue samples were taken and tissue weights, MDA and GSH levels were determined. Overall, as the generations progressed, a statistically significant increase in liver weights and a decrease in kidney weights were observed. There was no statistically significant change as regards MDA and GSH levels of liver and kidney tissues of groups without GM maize inclusion in the diet. However in all three-generation of GM maize treated groups, there was a significant decrease in GSH levels and a significant increase in MDA levels were observed.

The effect of Paracetamol exposure on hepatic and renal tissues during statin usage

2019 ◽  
Vol 44 (1) ◽  
pp. 113-120
Author(s):  
Saliha Aksun ◽  
Alpaslan Gökcimen ◽  
Fadime Kahyaoglu ◽  
Buket Demirci
Keyword(s):  
Drug Interaction ◽  
Combination Therapy ◽  
Male Rats ◽  
Control Group ◽  
Tissue Samples ◽  
Liver And Kidney ◽  
Statin Usage ◽  
Paracetamol Interaction ◽  
Oxide Synthase ◽  
Important Drug

Abstract Objective Statins and Paracetamol have widespread use in clinic and both drugs possess similar side effects; therefore, we investigated if drug-interaction occurs when the combination of these two drugs is used during therapy. Materials and methods A total of 32 (12–15 months old) grown-up male rats were divided into four groups: Control group, RSV group (10 mg/kg Rosuvastatin/daily), APAP group (50 mg/kg Paracetamol/5 days/weekly), RSV+APAP (10 mg/kg Rosuvastatin/daily+50 mg/kg Paracetamol/5 days/weekly). At the end of 8 weeks of chronic treatment, the blood and tissue samples were taken under the Ketamine and Xylasine anesthesia (50 mg/kg and 5 mg/kg, respectively). Results In the liver, sinusoidal dilatations, pyknotic nuclei and hemorrhagic foci are more frequently seen in the group receiving combination therapy; although serum liver functions among groups were not significantly different. Kidney histopathologic alterations in APAP and RSV+APAP groups were found more distinct than in RSV alone group. Inducible nitric oxide synthase activity was highly increased with combination therapy in liver and kidney tissues. Conclusion RSV-Paracetamol interaction may occur as an important drug interaction histopathologically even before it is manifested biochemically in the clinic.

Histopathological Effect of Pesticide Cypermethrin Toxicity on Liver and Gill of Freshwater Fish, Oreochromis Mossambicus (Tilapia)

GIS Business ◽  
2019 ◽  
Vol 14 (6) ◽  
pp. 163-169
Author(s):  
K. K. Kalpana Devi ◽  
J. Prakash Sahaya Leon
Keyword(s):  
Toxic Effect ◽  
Freshwater Fish ◽  
Cell Damage ◽  
Oreochromis Mossambicus ◽  
Sublethal Concentration ◽  
Pesticide Contamination ◽  
L Value ◽  
Gill Lamellae ◽  
Excessive Secretion ◽  
Length Reduction

Pollution is now a day has become a threat to living beings. Pollutants in the form of pesticide inhabit a variety of environments which affect the normal vital activities of organisms living. They reach the aquatic environment as a washout from agriculture fields as well as from industrial areas. Pesticide contamination in water may accumulate in the bodies of animals and affect the vital organs which alter the usual activities. Cypermethrin is a synthetic pyrethrum insecticide used in agriculture to control pest in crops such as lettuce and cotton and household pest such as cockroach. To understand the toxic effect of pesticides, it creates a need for histopathological observation of tissues. Present work was carried to evaluate the toxic effect of cypermethrin (25% EC) in liver and gills of tilapia (O.mossambicus). The fish experimented to different sublethal concentration of 1/10th,1/20th and 1/30th level of LC50 (4.5 mg/l) value of cypermethrin for 30 days. Gill exhibited epithelial cell damage, Fusion , Length reduction , Curling and Repturing of secondary gill lamellae, primary gill lamellae Enlargement, Necrosis, Cytoplasmic vacuolization, Damaged red blood corpuscle, Excessive secretion of mucus, whereas liver showed increased vacuolization, Necrosis, Hyperplasia, disintegration of hepatocytes and necrotic nuclei. Aim of the present study is to identify the effect of cypermethrin on organs tissues ( liver, kidney) of   freshwater fish, Oreochromis mossambicus. Keywords: Cypermethrin, Oreochromis mossambicus, Gill, Liver

Acetaminophen: Neonatal hepatotoxicity due to late pregnancy exposure

1987 ◽  
Vol 45 ◽  
pp. 718-719
Author(s):  
G.A. Miranda ◽  
M.A. Arroyo ◽  
C.A. Lucio ◽  
M. Mongeotti ◽  
S.S. Poolsawat
Keyword(s):  
Low Dose ◽  
Fetal Liver ◽  
Late Pregnancy ◽  
Toxic Chemicals ◽  
Control Group ◽  
High Dose ◽  
Over The Counter ◽  
Liver And Kidney ◽  
Neonatal Liver ◽  
Childbearing Women

Exposure to drugs and toxic chemicals, during late pregnancy, is a common occurrence in childbearing women. Some studies have reported that more than 90% of pregnant women use at least 1 prescription; of this, 60% used more than one. Another study indicated that 80% of the consumed drugs were not prescribed, and of this figure, 95% were “over-the-counter” drugs. Acetaminophen, the safest of all over-the-counter drugs, has been reported to induce fetal liver necrosis in man and animals and to have abortifacient and embryocidal action in mice. This study examines the degree to which acetaminophen affects the neonatal liver and kidney, when a fatty diet is simultaneously fed to the mother during late pregnancy.Timed Swiss Webster female mice were gavaged during late pregnancy (days 16-19) with fat suspended acetaminophen at a high dose, HD = 84.50 mg/kg, and a low dose, LD = 42.25 mg/kg; a control group received fat alone.

CLINICAL SIGNIFICANCE OF SERUM CAVEOLIN-1 LEVELS IN GASTRIC CANCER PATIENTS

2018 ◽  
Vol 40 (4) ◽  
pp. 323-327 ◽  
Author(s):  
F Tas ◽  
S Karabulut ◽  
K Erturk ◽  
D Duranyildiz
Keyword(s):  
Gastric Cancer ◽  
Cancer Patients ◽  
Histological Grade ◽  
Clinical Importance ◽  
Disease Stage ◽  
Control Group ◽  
Tissue Samples ◽  
Caveolin 1 ◽  
Study Results ◽  
Gastric Cancer Patients

Aim: Caveolin-1 plays a significant role in the pathogenesis of various carcinomas and its expression affects the survival of cancer patients. However, the molecular function of caveolin-1 and its possible clinical importance has remained uncertain in gastric cancer. No clinical trial has examined serum caveolin-1 levels in gastric cancer patients so far, instead all available results were provided from studies conducted on tissue samples. In the current study, we analyzed the soluble serum caveolin-1 levels in gastric cancer patients, and specified its associations with the clinical factors and prognosis. Material and Methods: Sixty-three patients with pathologically confirmed gastric cancer were enrolled into the trial. Serum caveolin-1 concentrations were detected by ELISA method. Thirty healthy subjects were also included in the study. Results: The median age of patients was 62 years, ranging from 28 to 82 years. The serum caveolin-1 levels in gastric cancer patients were significantly higher than those in control group (p < 0.001). The common clinical parameters including patient age, sex, lesion localization, histopathology, histological grade, disease stage, and various serum tumor markers (e.g. LDH, CEA, and CA 19.9) were not found to be associated with serum caveolin-1 levels (p > 0.05). Similarly, no correlation existed between serum caveolin-1 concentration and chemotherapy responsiveness (p = 0.93). Furthermore, serum caveolin-1 level was not found to have a prognostic role (p = 0.16). Conclusion: Even though it is neither predictive nor prognostic, serum caveolin-1 level may be a valuable diagnostic indicator in patients with gastric cancer. Key

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