scholarly journals UJI DAYA HAMBAT EKSTRAK DAUN CENGKEH (Syzygium aromaticum (L.) Merr. & L. M Perry.) TERHADAP PERTUMBUHAN BAKTERI Shigella dysenteriae

Biocelebes ◽  
2020 ◽  
Vol 14 (2) ◽  
pp. 119-129
Author(s):  
Iis Salihat ◽  
Orryani Lambui ◽  
Ramadanil Pitopang

Research about the inhibitory test of Syzygium aromaticum leaf extract on the growth of Shigella dysenteriae bacteria which aims to determine the effectiveness of S. aromaticum leaf extract in inhibited the growth of S. dysenteriae bacteria, knowing the concentration of leaf extract is effective in inhibited the growth of S. dysenteriae bacteria and the content of flavonoid compounds, saponins, tannins and alkaloids contained in leaves S. aromaticum, has been conducted from July until December 2016. Extraction method used is Maseration method. Inhibitory test extract on S. dysenteriae bacteria using disc diffusion method. This research was arranged in Completely Randomized Design with 6 treatments and 3 repetitions with extract concentration 30%, 45%, 60% and 75% and 2% Cotrimoxazole antibiotics as positive controls and Aquades as negative controls. The results showed that 75% extract concentration of S. aromaticum plant produced the largest drag zone compared to other concentrations of 17 mm. This indicates that S. aromaticum leaf extract has good inhibitory ability against S. dysenteriae bacteria.

2020 ◽  
Vol 9 (3) ◽  
pp. 262
Author(s):  
Aditya Yusril Hidayat ◽  
Agus Selamet Duniaji ◽  
Komang Ayu Nocianitri

The aim of this research to determine the ability of mulberry leaf extract (Morus alba) to inhibit the growth of Aspergillus flavus. This research used completely Randomized Design with control treatment (without extract) and treatment of mulberry leaf extract concentration of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100%. The experiment were repeated 2 times and resulting in 22 experimental units. The data were presented in descriptive statistic with tables, and figure. The results showed that the extract of mulberry leaf inhibit the growth of Aspergillus flavus with optimal concentration 80% with an average 11.95 mm and strong inhibition categories.


2020 ◽  
Vol 13 (2) ◽  
pp. 253
Author(s):  
Nonice Manikome ◽  
Morina Handayani

In terms of control, various natural chemical compounds derived from plants can be used. Like papaya and soursop leaves, the formulation of the problem is whether the combination of soursop leaf extract and papaya leaf extract is effective in controlling S. litura pests and what is the most effective extract concentration. This study used a completely randomized design (RBD) with 5 treatments and 5 replications. Observation variables included symptoms, morphological changes and mortality, the results were analyzed by Analysis of Variance (ANOVA) followed by the Least Significant Difference (LSD) 5% test. The initial symptoms that were seen one day after application of the combination were morphological changes in the test larvae. Such as damage to the body of the larva, change in color, then the larva's body shrinks, when touched the larvae easily fall. The highest mortality was found at the extract concentration of 20% with a mortality rate of 64.79% on the second day. The results showed that the high mortality rate was closely related to the high toxicity contained in the 20% extract concentration. It was concluded that the results of the effectiveness test of the combination of papaya leaf extract and soursop leaf extract were effective. Suggestions for the use of natural materials that are easily available can be used as an alternative to control pests that are environmentally friendly. Furthermore, it is suggested that if you want to increase mortality, you can try increasing the extract concentration.


2021 ◽  
Vol 10 (1) ◽  
pp. 93-101
Author(s):  
Sri Lestari Ramadhani Nasution ◽  
Sri Wahyuni Nasution ◽  
Ali Napiah Nasution

ABSTRAKDaun salam (Syzygium polyanthum) banyak digunakan sebagai obat tradisional untuk mengobati berbagai macam penyakit, salah satunya sebagai antimikroba. Penelitian ini bertujuan untuk mengetahui aktivitas antimikroba dan konsentrasi efektif dari ekstrak daun salam (S. polyanthum) terhadap Pityrosporum ovale. Pengujian menggunakan rancangan acak lengkap faktorial dan metode pengujian antijamur dengan metode disc diffusion. Data diolah dengan SPPS versi 23. Konsentrasi ekstrak daun salam (S. polyanthum) yang digunakan yakni 25%, 50%, 75%, dan 100%. Hasil penelitian menunjukkan adanya potensi antimikroba pada semua konsentrasi ekstrak daun salam (S. polyanthum) yang ditunjukkan dengan zona bening di sekitar paper disk. Zona bening terbesar adalah 18,2 mm dan 15,03 mm diperoleh dari ekstrak daun salam (S. polyanthum) dengan konsentrasi 100%. Kata kunci: antifungi; disc diffusion; Pityrosporum ovale; Syzygium polyanthum ABSTRACTEffectiveness of salam leaves (Syzygium polyanthum) extract against Pityrosporum ovaleBayleaf (Syzygium polyanthum) is a plant that is used as a traditional medicine to treat various diseases. One of them is as an antimicrobial. This study aims to determine the antimicrobial activity and effective concentration of bayleaf extract against P. ovale. This research use a factorial completely randomized design and the antifungal disc diffusion method. The data were processed using SPPS version 23. The bayleaf (S. polyanthum) extract concentrations are 25%, 50%, 75%, and 100%. The results showed the presence of antimicrobial potential in all concentration of bayleaf (S. polyanthum) extracts indicated by the clear zone around the paper disk. The largest clear zones were 18.2 mm and 15.03 mm obtained 100% bayleaf (S. polyanthum) extracts. Keywords: antifungus; disc diffusion; Pityrosporum ovale; Syzygium polyanthum


2021 ◽  
Vol 26 (1) ◽  
pp. 62
Author(s):  
Mariana Lumban Tungkup ◽  
Henni Syawal ◽  
Morina Riauwaty

This research has been carried out from November to December 2019 at the Laboratory of Parasites and Fish Diseases at the Fisheries and Marine Faculty of Riau University. The aim of the study was to obtain the best dose of mango leaf solution for the treatment of striped catfish (P. hypophthalmus) infected with E. tarda, seen from the erythrocyte picture. The method used was an experiment with a Completely Randomized Design (CRD) of a factor of five treatment levels and replications three times. Test fish used were striped catfish measuring 8-12 cm, with a total of 150 fish. The treatments used for treatment were 1000 ppm, 1200 ppm, 1400 ppm, negative controls (not infected and not treated), and positive controls (infected and not treated). Parameters measured and observed among others; total erythrocytes, hemoglobin levels, hematocrit values, erythrocyte cell morphology and survival rate. The results showed that apple mango leaf solution (M. indica) was able to treat conjoined striped catfish infected with E. tarda by Emmersion method. The best treatment of 1200 ppm is characterized by an average total erythrocyte, mean total erythrocytes 149.33 x 104 cells / mm3, hemoglobin level 9.03 g / dL, hematocrit value 35.67% and the survival of the test fish after treatment is 86.67%. The results of water quality measurements during the study, namely temperature 27.00-27.46 0C, pH 6.1-6.77, dissolved oxygen 3.53-3.87 mg/L, and ammonia 0.02 mg/L.


2019 ◽  
Vol 19 (2) ◽  
pp. 99
Author(s):  
Finka Bella Palit ◽  
Henny Lieke Rampe ◽  
Marhaenus Rumondor

Penelitian ini bertujuan untuk mengevaluasi intensitas serangan akibat hama pemakan daun setelah aplikasi ekstrak daun kirinyuh pada tanaman sawi. Penelitian ini dilaksanakan di lahan percobaan di Kelurahan Lansot Kecamatan Tomohon Selatan Kota Tomohon pada bulan Desember 2018 – Februari 2019. Penelitian ini menggunakan rancangan acak lengkap (RAL) yang terdiri dari 4 perlakuan konsentrasi ekstrak daun kirinyuh yaitu K0 (kontrol) = 0 g/L air, K1  = 100 g/L air, K2 = 200 g/L air, dan K3 = 300 g/L air. Intensitas serangan diamati berdasarkan luas area daun yang dirusak oleh hama pemakan daun pada daun sawi setelah aplikasi ekstrak daun krinyuh. Hasil penelitian menunjukkan bahwa pemberian ekstrak daun kirinyuh berpengaruh nyata terhadap intensitas serangan pada tanaman sawi dan konsentrasi ekstrak daun kirinyuh yang dapat menekan serangan hama pemakan daun yaitu yang tertinggi pada perlakuan K3 (300 g/L air).Kata kunci: Tanaman sawi (Brassica juncea L.), intensitas serangan, tumbuhan kirinyuh (Chromolaena odorata) THE ATTACK INTENSITY DUE TO LEAF-EATING PESTS AFTER APPLICATION OF KIRINYUH (Chromolaena odorata L.) LEAF EXTRACT ON MUSTARD GREEN  (Brassica juncea L.) ABSTRACTThis study aimed to evaluate the intensity of the attack due to leaf-eating pests after the application of kirinyuh leaf extract on mustard greens. This research was carried out on experimental fiel in Lansot Sub-District, South Tomohon  District, Tomohon City in December 2018-February 2019. This study was used a completely randomized design consisting of 4 treatments of kirinyuh leaf extract concentration, namely K0 (control) = 0 g / L water, K1 = 100 g / L water, K2 = 200 g / L water, and K3 = 300 g / L water. The intensity of the attack was observed based on the area of leaf damaged by pest. The results showed that the application of kirinyuh leaf extract significantly affected the intensity of attacks on mustard greens and the concentration of kirinyuh leaf extract which was able to suppress leaf-eating pests which was the highest in the K3 treatment (300 g/L water).Keywords: Mustard greens (Brassica juncea L.), intensity of attack, Kirinyuh  (Chromolaena odorata)


2019 ◽  
Vol 2 (1) ◽  
Author(s):  
Anti Uni Mahanani ◽  
Lemira Kogova

ABSTRACT Vegetables are one of the agricultural products that are needed and this annual crop is a plant that is generally relatively little time to plant. Of the various types of vegetables, including lettuce. To get good results, both in quality and quantity for lettuce, one of the efforts that can be taken apart from the provision of Growth Regulatory Substances (ZPT). With a good ZPT, good lettuce plants will be produced both in terms of quality and quantity. One of these ZPT is Moringa leaf extract. The purpose of this study was to determine the effect of the treatment of Moringa leaf extract concentration on the growth and yield of lettuce plants and to determine the concentration of Moringa leaf extract which is best used to increase the growth and yield of lettuce plants. The study was conducted at the STIPER Experimental Garden Petra Baliem Wamena Jayawijaya Regency at an altitude of 1664 meters above sea level. The study was conducted in June to September 2017. Materials used were: Lettuce Seeds, Moringa Leaves, Polybags, Soil. The tools used are: Digital Scales, Stationery, Blenders, Strainer, Measuring Cup. The study used a Completely Randomized Design (CRD) consisting of 4 levels of concentration of Moringa leaf extract treatment and 4 replications. The level of treatment of Moringa leaf extract concentration are: K0: 0% concentration (300 ml water without Moringa leaf extract); K1: 25% concentration (75 ml of Moringa leaf extract + 225 ml of water); K2: 50% concentration (150 ml moringa leaf extract + 150 ml water); K3: 75% concentration (225 ml moringa leaf extract + 75 ml water). In this study there were 4 (four) treatments and 4 (four) replications so that there were 16 plots / units of the experiment. The results of this study are: 1) The concentration of Moringa leaf extract can affect lettuce height at 1 week, 2 weeks and 4 weeks; number of leaves of lettuce at 2 weeks, 3 weeks and 4 weeks; and fresh weight of lettuce. and 2) The best concentration of Moringa leaf extract for growth and yield of lettuce is K2 concentration (50% concentration).Keywords: concentration, moringa leaf extract, lettuce ABSTRAK Sayuran merupakan salah satu produk pertanian yang sangat dibutuhkan dan  tanaman semusim ini merupakan tanaman yang pada umumnya relatif sedikit waktu tanamnya. Dari berbagai jenis sayuran itu, termasuk didalamnya adalah sayur selada. Untuk memperoleh hasil yang baik, baik secara kualitas maupun kuantitas bagi tanaman selada, salah satu usaha yang dapat ditempuh selain dengan pemberian Zat Pengatur Tumbuh (ZPT). Dengan ZPT yang baik, maka akan dihasilkan tanaman selada yang bagus baik dari segi kualitas maupun kuantitasnya. Salah satu ZPT tersebut adalah ekstrak daun kelor. Tujuan penelitian ini adalah Untuk mengetahui pengaruh perlakuan konsentrasi ekstrak daun kelor terhadap pertumbuhan dan hasil tanaman selada dan Untuk mengetahui konsentrasi ekstrak daun kelor yang paling baik digunakan untuk meningkatkan pertumbuhan dan hasil tanaman selada. Penelitian dilaksanakan di Kebun Percobaan STIPER Petra Baliem Wamena Kabupaten Jayawijaya pada ketinggian tempat 1664 mdpl. Penelitian dilaksanakan pada bulan Juni s/d September 2017. Bahan yang digunakan adalah : Benih Selada, Daun Kelor, Polybag, Tanah. Alat yang digunakan adalah : Timbangan Digital, Alat Tulis, Blender, Saringan, Gelas Ukur. Penelitian menggunakan Rancangan Acak Lengkap (RAL) yang terdiri atas 4 aras perlakuan konsentrasi ekstrak daun kelor dan 4 ulangan. Adapun aras perlakuan konsentrasi ekstrak daun kelor adalah : K0: Konsentrasi 0% ( 300 ml air tanpa ekstrak daun kelor ); K1:Konsentrasi 25% ( 75 ml ekstrak daun kelor + 225 ml air ); K2: Konsentrasi 50% ( 150 ml ekstrak daun kelor + 150 ml air ); K3: Konsentrasi 75% ( 225 ml ekstrak daun kelor + 75 ml air ). Pada penelitian ini ada 4 (empat) perlakuan dan 4 (empat) ulangan sehingga terdapat 16 petak/unit percobaan. Hasil dari penelitian ini adalah : 1) Konsentrasi ekstrak daun kelor dapat mempengaruhi tinggi tanaman selada umur 1 minggu, 2 minggu, dan 4 minggu;  jumlah daun tanaman selada umur 2 minggu, 3 minggu dan 4 minggu; dan bobot segar tanaman selada. dan 2) Konsentrasi ekstrak daun kelor yang terbaik bagi pertumbuhan dan hasil tanaman selada adalah konsentrasi K2 (konsentrasi 50%).Kata kunci : konsentrasi, ekstrak daun kelor, selada


2021 ◽  
Vol 1 (2) ◽  
pp. 89-94
Author(s):  
Vivin Diana Putri ◽  
Etik M Achadian

The white fly explosion might be occurred on the flooded fields and lack of nitrogen fertilizer. Some control methods have to be conducted to prevent further losses. The use of biopesticide was known to be easier, cheaper and environmental friendly control methods. Therefore the aim of this experiment was determining the effect of white champa leaf extract (Plumeria acuminata) as a biopesticide on sugarcane white fly eclosion (Aleurolobus barodensis). Experiment was conducted in the laboratory, using a completely randomized design with 6 treatments of P. acuminata leaf extract concentrations, that were 0% (control), 10%, 20%, 30%, 40% and 50%, and was replicated 4 times. The results showed that P. acuminata leaf extract could be used to control sugarcane white fly. The effective leaf extract concentration was 40%. The results also proved that P. acuminata leaf extract killed the white fly pupae instead of delaying their eclosion.


2021 ◽  
Vol 3 (2) ◽  
pp. 85
Author(s):  
Sri Sukaryani ◽  
Engkus Ainul Yakin ◽  
Hardika Eka Rhamadanu

 Duck eggs are one type of protein source food that is much favored by the people of Indonesia.  However, currently there are not many ways of preservation to maintain the quality of duck eggs in storage.  With preservation by immersion with noni leaf extract, it can be an alternative in maintaining the quality of duck eggs in storage for 15 days.  This is because noni leaves contain elements of chemical compounds such as amino acids, phenolic compounds, ursulic acid, alkaloids, phenols, and glycosides which are antimicrobial, antibacterial and anti-inflammatory.  This study aims to determine the effect of soaking duck eggs in noni leaf extract on the interior quality of duck eggs.  This study used a completely randomized design (CRD) with a concentration of 45% noni leaf extract with immersion time of 0, 12, 24, 36 and 48 hours with 3 replications.  To determine whether there is a difference in treatment using Duncan Multiple Range Test (DMRT) at the level of = 0.05.  The research variables are Albumen Insex, Yolk Index and Haugh Index.  Data were analyzed by SPSS.  The results of the study concluded that soaking duck eggs with noni leaf extract for 12-48 hours with an extract concentration of 45% had no significant effect on egg quality in this case the yolk index, albumen index and Haugh index.


2016 ◽  
Vol 6 (2) ◽  
Author(s):  
Midy San Lebang ◽  
Dantje Taroreh ◽  
Jimmy Rimbing

Abstrak Penelitian ini bertujuan menguji efektifitas ekstrak daun sirsak dan daun gamal sebagai pengendali hama walang sangit  pada tanaman padi. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 2 perlakuan yaitu jenis ekstrak dan konsentrasi ekstrak. Perlakuan 1 yaitu jenis ekstrak daun (A): sirsak (A1) dan gamal (A2), perlakuan 2 yaitu konsentrasi ekstrak (K): Kontrol (K0), 5% (K1), 10% (K2), 15% (K3), dan 20% (K4). Hasil penelitian menunjukkan bahwa ekstrak daun sirsak efektif terhadap mortalitas walang sangit tertinggi pada konsentrasi 20% (83%) dan yang terendah pada konsentrasi 0% (0 %). Ekstrak daun gamal juga efektif terhadap mortalitas walang sangit tertinggi pada konsentrasi 20% (74%) dan yang terendah pada konsentrasi 0% (0%). Waktu kematian pada hari pertama setelah aplikasi dengan rata-rata mortalitas tertinggi berada pada perlakuan ekstrak daun sirsak 20% (30%) dan daun gamal 20% (28%) dan terendah berada pada perlakuan ekstrak daun sirsak 0% (0%) dan daun gamal 0 % (0%). Kata kunci: daun gamal, daun sirsak, tanaman padi, walang sangit Abstract This study aimed to evaluate the effectiveness of soursop leaf extract and gliricidia leaves as pest control of walang rice (L. acuta) in the rice plants. This study used a completely randomized design (CRD) with 2 treatments, i.e. extract type and extract concentration. The treatment of extract type (A) were soursop (A1) and Gamal (A2), whereas the treatment  of extract concentration (K) were control (K0), 5% (K1), 10% (K2), 15% (K3), and 20% (K4). The results showed that the soursop leaf extract was effective to control sangit walang with the highest mortality was in the concentration of 20% (83%) and the lowest was in the concentration of 0% (0%). Gliricidia leaf extract was also effective to control sangit walang with the highest mortality in the concentration of 20% (74%) and the lowest was in the concentration of 0% (0%). The mortality time was on the first day of the application with the average highest mortality was in the treatment of 20% soursop leaf extract  (30%)and 20% gliricidia leaves (28%) respectively. The lowest mortality was in the treatment of 0% soursop leaf extract (0%) and 0% gliricidia leaves (0%).Keywords: gliricidia leaves, rice plant, soursop leaves, walang rice pest


AGRICA ◽  
2019 ◽  
Vol 1 (2) ◽  
pp. 32-37
Author(s):  
Yustina M.S.W Puu ◽  
Hildegardis Nalti Nansi

Callosobruchus Chinensis is a pest that damages mung bean seeds in storage and causes damage to both the quality and quantity of seeds. This study aims to determine the effectiveness of neem leaf extracts in suppressing the development of the Callosobruchus Chinensis pest as one of the postharvest pests in the commodity green beans. This research conducted at the Laboratory of the Faculty of Agriculture, University of Flores, by using a Completely Randomized Design (CRD) with five treatments and three replications. The concentration of neem leaf extract treatment is 0 ml / l, 25 ml / l, 30 ml / l, 35 ml / l, and 40 ml / l. The results showed that the concentration of neem leaf extract 40 ml / l caused mortality of C. Chinensis imago as contact poison by 99% and nerve poison by 47%. While the effectiveness of the limb leaf extract on spawning activity was 86%.


Sign in / Sign up

Export Citation Format

Share Document