scholarly journals KEMAMPUAN DAYA HAMBAT EKSTRAK DAUN MURBEI (Morus alba) TERHADAP PERTUMBUHAN Aspergillus flavus

2020 ◽  
Vol 9 (3) ◽  
pp. 262
Author(s):  
Aditya Yusril Hidayat ◽  
Agus Selamet Duniaji ◽  
Komang Ayu Nocianitri

The aim of this research to determine the ability of mulberry leaf extract (Morus alba) to inhibit the growth of Aspergillus flavus. This research used completely Randomized Design with control treatment (without extract) and treatment of mulberry leaf extract concentration of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100%. The experiment were repeated 2 times and resulting in 22 experimental units. The data were presented in descriptive statistic with tables, and figure. The results showed that the extract of mulberry leaf inhibit the growth of Aspergillus flavus with optimal concentration 80% with an average 11.95 mm and strong inhibition categories.

2017 ◽  
Vol 3 (1) ◽  
pp. 43
Author(s):  
Nurul Arfiyanti Yusuf ◽  
Aisyah Fatmawaty

The research has conducted research on the effectiveness of isopropyl myristat as a penetration enhancer on the diffusion rate of whitening cream mulberry leaf extract (Morus alba L) in vitro. This study aims to determine the effect of the use of isopropyl myristat. Mulberry leaf extract cream made with varying concentrations respectively 3%, 4%, 5% Isopropyl myristat as penetration enhancers made into 3 formulas (F1-F4) with the F1 without penetration enhancers. Evaluation of stability before and after accelerated storage includes observation of the organoleptic, emulsion type determination, measurement of pH, and viscosity. The evaluation results indicate four physically stable formula. In vitro diffusion studies conducted by Franz diffusion cells and footage is measured at a wavelength of 367.4 nm. The results of diffusion studies show that formula with the highest diffusion rate of 0.024 µg/minute on F4 (5% isopropyl myristat).


Biocelebes ◽  
2020 ◽  
Vol 14 (2) ◽  
pp. 119-129
Author(s):  
Iis Salihat ◽  
Orryani Lambui ◽  
Ramadanil Pitopang

Research about the inhibitory test of Syzygium aromaticum leaf extract on the growth of Shigella dysenteriae bacteria which aims to determine the effectiveness of S. aromaticum leaf extract in inhibited the growth of S. dysenteriae bacteria, knowing the concentration of leaf extract is effective in inhibited the growth of S. dysenteriae bacteria and the content of flavonoid compounds, saponins, tannins and alkaloids contained in leaves S. aromaticum, has been conducted from July until December 2016. Extraction method used is Maseration method. Inhibitory test extract on S. dysenteriae bacteria using disc diffusion method. This research was arranged in Completely Randomized Design with 6 treatments and 3 repetitions with extract concentration 30%, 45%, 60% and 75% and 2% Cotrimoxazole antibiotics as positive controls and Aquades as negative controls. The results showed that 75% extract concentration of S. aromaticum plant produced the largest drag zone compared to other concentrations of 17 mm. This indicates that S. aromaticum leaf extract has good inhibitory ability against S. dysenteriae bacteria.


2021 ◽  
Vol 7 (1) ◽  
pp. 1
Author(s):  
Gama Wisnu Sanjaya ◽  
Cicih Komariah ◽  
Dini Agustina

Conjunctivitis is inflammation in the conjunctiva, with clinical signs of exudation, cellular infiltration, and vascular dilatation. In adults, Staphylococcus species are the most common pathogenic bacteria resulting in bacterial conjunctivitis. Previous studies in vitro, explaining that mulberry leaf extract can inhibit the growth of S. aureus bacteria. The purpose of this study was to investigate the effect of mulberry leaf ethanol extract (Morus alba L.) on wistar mice conjunctivitis model by S. aureus. This type of research is true experimental with pretest-posttest control group design. The 24-wistar mice were adapted for 7 days. On day 8, a conjunctivitis induction was performed with 1.5 x 108 CFU / ml of S. aureus at 1 drop every 10 minutes for 1 hour (left 3 days) until conjunctivitis . On day 12 until day 19 (7 days), given mulberry leaf extract 45%, 55%, 65% and 75%. The results of TPC S. aureus logarithm on MSA medium of the 11th (pretest) day conjunctival swab and the 17th day (posttest) of rat adaptation were K (-) (5.70 and 4.07); K (+) (5.78 and 3.31); P1 (5.65 and 3.78); P2 (5.63 and 3.82); P3 (5.84 and 3.74); and P4 (5.69 and 3.63), with the posttest group there was improvement in conjunctivitis symptoms rather than the pretest group. The result of One Way Way Anova data analysis showed significant result at pretest p = 0,002 and posttest p = 0,046 (p <0,05) and paired t-test got significant result p = 0,000 (p <0,05). From this study it can be concluded that mulberry leaf extract (Morus alba L.) has the effect of decreasing the number of colonies of S. aureus bacteria and improving symptoms of conjunctivitis. Keywords: mulberry leaf extract, conjunctivitis, S. aureus


Author(s):  
Ahmad Irsyad Aliah ◽  
Wahyuni Wahyuni ◽  
Nurjannah Bachri

A research on the inhibition of formula gel test ethanol extract of leaves of mulberry (Morus alba L.) as an anti-acne against bacteria Propionibacterium acne. This study uses a gel formulation made using mulberry leaf extract (Morus alba L.), which aims to determine the inhibition of antibacterial gel mulberry leaf extract (Morus alba L.) against the bacteria Propionibacterium acne. Gel made three dosage concentrations of 2%, 4% and 6%. Testing inhibition of antibacterial gel agar diffusion method. Test Antibacterial inhibition obtained by looking at the clear zone in the medium and measured using calipers. The results showed that the inhibition of antibacterial gel formulation with a concentration of 2%, 4% and 6% showing that inhibiting bacterial activity.


2020 ◽  
Vol 4 (2) ◽  
pp. 48-53
Author(s):  
Muhammad Nur Hidayat ◽  
Amina Hajah Thaha ◽  
Reny Mayanti

This study aims to determine the effect of noni leaf (Morinda citrifolia lignosae) extract as a natural disinfectant on the percentage of hatchability and the hatchability weight of quail (Coturnix-coturnix japonica). This study used 1,500 quail hatch eggs with a male and female parent ratio of 1: 4. This study used a Completely Randomized Design (CRD) method, with five treatments and three replications each repetition consisting of 100 quail eggs with 10-gram weight. The treatments used were: T0 (control treatment), T1 (commercial disinfectant), T2 (noni leaf extract 10%), T3 (noni leaf extract 20%) and T4 (noni leaf extract 30%). The results of variance showed the average percentage of hatchability produced during the study, namely T0 (85%), T1 (83%), T2 (86%), T3 (84%), and T4 (93%). Furthermore, the average weight of hatchability produced, namely T0 (6.67 grams), T1 (6.76 grams, T2 (6.89 grams), T3 (6.84 grams) and T4 (6.89 grams). Overall administration of noni leaf extract had no significant effect (P> 0.05) on the percentage of hatchability and DOQ hatching weight of the quail.


PLoS ONE ◽  
2021 ◽  
Vol 16 (12) ◽  
pp. e0260030
Author(s):  
Agnieszka Bilska

Mulberry (Morus alba L.), and above all the extract from the leaves of this plant, is a natural medicine that has been used in traditional medicine for hundreds of years. Mulberry leaves contains polyphenol compounds: flavonoids, coumarins, numerous phenolic acids, as well as terpenes and steroids. The antioxidant effect of these compounds may be beneficial to the fat fraction of meat products, thereby increasing their functional qualities. The aim of the study was to evaluate the effectiveness of the use of mulberry water leaf extract, as an additive limiting adverse fat changes and affecting the functionality in model liver pâtés. Pork pâtés were prepared by replacing 20% of animal fat with rapeseed oil (RO), and water extract of mulberry leaves was added in the proportion of 0.2%, 0.6% and 1.0%. It has been shown that the addition of mulberry leaf extract delayed the appearance of primary and secondary fat oxidation products. The most effective antioxidant effect during 15-day storage was observed in the sample with the addition of 0.6% and 1.0% water mulberry leaf extract. These samples also showed inhibiting activity against angiotensin-converting enzymes and cholinesterase’s. During storage, the tested pâtés had a high sensory quality with unchanged microbiological quality. Mulberry leaf extract can be an interesting addition to the production of fat meat products, delaying adverse changes in the lipid fraction and increasing the functionality of products.


2020 ◽  
Vol 13 (2) ◽  
pp. 253
Author(s):  
Nonice Manikome ◽  
Morina Handayani

In terms of control, various natural chemical compounds derived from plants can be used. Like papaya and soursop leaves, the formulation of the problem is whether the combination of soursop leaf extract and papaya leaf extract is effective in controlling S. litura pests and what is the most effective extract concentration. This study used a completely randomized design (RBD) with 5 treatments and 5 replications. Observation variables included symptoms, morphological changes and mortality, the results were analyzed by Analysis of Variance (ANOVA) followed by the Least Significant Difference (LSD) 5% test. The initial symptoms that were seen one day after application of the combination were morphological changes in the test larvae. Such as damage to the body of the larva, change in color, then the larva's body shrinks, when touched the larvae easily fall. The highest mortality was found at the extract concentration of 20% with a mortality rate of 64.79% on the second day. The results showed that the high mortality rate was closely related to the high toxicity contained in the 20% extract concentration. It was concluded that the results of the effectiveness test of the combination of papaya leaf extract and soursop leaf extract were effective. Suggestions for the use of natural materials that are easily available can be used as an alternative to control pests that are environmentally friendly. Furthermore, it is suggested that if you want to increase mortality, you can try increasing the extract concentration.


2019 ◽  
Vol 19 (2) ◽  
pp. 99
Author(s):  
Finka Bella Palit ◽  
Henny Lieke Rampe ◽  
Marhaenus Rumondor

Penelitian ini bertujuan untuk mengevaluasi intensitas serangan akibat hama pemakan daun setelah aplikasi ekstrak daun kirinyuh pada tanaman sawi. Penelitian ini dilaksanakan di lahan percobaan di Kelurahan Lansot Kecamatan Tomohon Selatan Kota Tomohon pada bulan Desember 2018 – Februari 2019. Penelitian ini menggunakan rancangan acak lengkap (RAL) yang terdiri dari 4 perlakuan konsentrasi ekstrak daun kirinyuh yaitu K0 (kontrol) = 0 g/L air, K1  = 100 g/L air, K2 = 200 g/L air, dan K3 = 300 g/L air. Intensitas serangan diamati berdasarkan luas area daun yang dirusak oleh hama pemakan daun pada daun sawi setelah aplikasi ekstrak daun krinyuh. Hasil penelitian menunjukkan bahwa pemberian ekstrak daun kirinyuh berpengaruh nyata terhadap intensitas serangan pada tanaman sawi dan konsentrasi ekstrak daun kirinyuh yang dapat menekan serangan hama pemakan daun yaitu yang tertinggi pada perlakuan K3 (300 g/L air).Kata kunci: Tanaman sawi (Brassica juncea L.), intensitas serangan, tumbuhan kirinyuh (Chromolaena odorata) THE ATTACK INTENSITY DUE TO LEAF-EATING PESTS AFTER APPLICATION OF KIRINYUH (Chromolaena odorata L.) LEAF EXTRACT ON MUSTARD GREEN  (Brassica juncea L.) ABSTRACTThis study aimed to evaluate the intensity of the attack due to leaf-eating pests after the application of kirinyuh leaf extract on mustard greens. This research was carried out on experimental fiel in Lansot Sub-District, South Tomohon  District, Tomohon City in December 2018-February 2019. This study was used a completely randomized design consisting of 4 treatments of kirinyuh leaf extract concentration, namely K0 (control) = 0 g / L water, K1 = 100 g / L water, K2 = 200 g / L water, and K3 = 300 g / L water. The intensity of the attack was observed based on the area of leaf damaged by pest. The results showed that the application of kirinyuh leaf extract significantly affected the intensity of attacks on mustard greens and the concentration of kirinyuh leaf extract which was able to suppress leaf-eating pests which was the highest in the K3 treatment (300 g/L water).Keywords: Mustard greens (Brassica juncea L.), intensity of attack, Kirinyuh  (Chromolaena odorata)


2018 ◽  
Vol 4 (3) ◽  
pp. 153
Author(s):  
Ema Fawziyah Ulfah ◽  
Cicih Komariah ◽  
Ulfa Elfiah

Cataract is a degenerative disease and one of the leading causes of blindness in Indonesia and in the world. Free radical formation leads to pathological reactions in the lens that can induce lipid peroxidation that produces malondialdehyde (MDA). Mulberry leaf (Morus alba L.) is one of the plants rich in antioxidants. The purpose of this study was to determine the effect and effective dose of mulberry leaf extract on MDA content of eye lens in rats cataract model. This study is true experimental with posttest only control group design. This study used 7 groups, each 5 tails. Normal control groups were not treated. The negative control group induced sodium selenite 25 μmol / kgBW and was given 0.1% HPMC eye drops. The treatment group induced sodium selenite 25 μmol / kgBB and given 0.05%, 0.1%, 0.2%, 0.3% and 0.4% mulberry leaf extract for 14 days. Measurement of MDA lens concentration are using MDA-TBA method. Data analyzed by using one way Anova test showed significant result with value p = 0,000 (p <0,05). Based on the results of this study it can be concluded that mulberry leaf extract has an effect on decreasing MDA lens in rats cataract model. Among the concentrations used, the concentrations that showed the lowest decrease in MDA concentration were 0.4%.Keywords: cataract, sodium selenite, malondialdehid, Morus alba L


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