scholarly journals Evaluation of The Effects of Different Remineralization agents on Initial Enamel Lesions by Scanning Electron Microscope and Energy-Distributed X-ray Analysis

Author(s):  
munevver kilic ◽  
Taskin Gürbüz

Aim: The present study compares the effectiveness of four different remineralization agents on the demineralized enamel of permanent human incisors. The purpose of this study is to evaluate the effectiveness of remineralization agents on the initial enamel lesion. Material- Methods: Crowns affected by demineralization were divided into two equal parts. The right halves of the teeth were subjected to no other processes after demineralization for control purposes and were kept in artificial saliva for the duration of the experiment. The left halves of the crowns were remineralized using a 5% NaF-containing fluoride varnish (GC MI Varnish GC Corp, Tokyo, Japan), a casein phosphopeptide and amorphous calcium toothpaste (GC Tooth Mousse, GC Corp, Tokyo, Japan), fluoride, hydroxyapatite, and xylitol, containing a water-soluble remineralization paste (Remin Pro™, Voco, Germany) and calcium, magnesium and phosphate-containing gel (Medical Remineralizing gel (R.O.C.S. Trading GmbH, Munich, Germany). After a 21-day remineralization process, and SEM/EDX analysis was performed, and a One Way Anova was used for statistical analysis. Results: According to EDX analysis, the R.O.C.S. medical remineralizing gel was found to have a lower Ca/P ratio than the other material groups (p =0.04). Remin Pro™, Voco, and the R.O.C.S. were higher than the control group in evaluating the Ca / P ratio (p=0.014), (p=0.025). Conclusions: The R.O.C.S group, treated with fluoride-free xylitol-containing, a remineralization agent, showed the lowest Ca/P ratio. Compared to demineralized halves of the teeth (control groups), Remin Pro™, Voco, and R.O.C.S. medical remineralizing gel may be considered as affecting on initial enamel lesions. Key Words: Remineralizing Agents, EDX, SEM, Enamel,

2020 ◽  
Author(s):  
Mariusz Konieczny ◽  
Paweł Pakosz ◽  
Mateusz Witkowski

Abstract Background: According to research, fast skating on short distances causes asymmetry in the physiology of muscle work. As has been proven in many sporting disciplines, this asymmetry can increase the risk of injury. The aim of the study was to analyze the level of right and left fatigue asymmetry of gluteus maximus muscle in elite skaters on a short track and to compare this phenomenon to a control group. The muscles were chosen deliberately, due to their influence in maintaining the right position during training on ice. Methods: The experiment compared a group of eight members of the Polish Women’s National Team in short track with a group of eight non-training people. The subjects did the Biering-Sorensen test, in which sEMG (surface electromyography) signal frequency was measured in the gluteus maximus muscles during an isometric contraction. Fatigue slopes were analyzed with a one-way ANOVA with repeated measures.. In the skaters, the fatigue differed between the right and the left gluteus maximus muscles. All the skaters had higher fatigue in the right leg. This phenomenon was not observed in the non-training subjects, who on average had similar fatigue in both legs. Results: The results suggest that professional short-track training leads to considerable asymmetry in fatigability of gluteus maximus muscles, thereby increasing the risk of injury in training and competition. Conclusions: Training should thus be planned in a way that minimizes the risk of causing muscle fatigue asymmetry in skaters despite the typically asymmetrical muscle work during training on ice and competition, thus new trainign protocols should be developed or considered to decrease that asymmetry. Trial registration: The tests were previously approved by the Bioethical Commission of the Chamber of Physicians in Opole. (Resolution No. 235 of 13 December 2016).


e-GIGI ◽  
2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Fransiska M. Pardosi ◽  
Darmawati A. Indraswari ◽  
Lusiana Batubara ◽  
Nadia Hardini

Abstract: Nanofiller composite resin has small filler size which enhances its properties. However, these properties could decrease due to several factors. Acidic liquid such as coffee could reduce the hardness of composite resin. This study was aimed to analyze the effect of robusta and arabica coffee immersion on the hardness of nanofiller composite resin. A total of 27 composite resin samples were used and then were polished by using a soflex disc for 30 seconds on each roughness level. Samples were then divided into three groups, as follows: the control group with artificial saliva and the treatment groups with robusta coffee and arabica coffee. The composite resin molds were immersed in the three groups for 5 days. After five days of immersion, the hardness of the sample was tested by using a Vickers hardness tester. Artificial saliva as the control group had the highest mean hardness value of 112.98±8.67 VHN, followed by robusta coffee, and then by arabica coffee. The One Way Anova and post hoc LSD test showed that there were significant differences in all groups (p<0.05) except for the two treatment groups, namely robusta and arabica coffee groups which did not show any significant difference in the resin hardness (p>0.05). In conclusion, robusta and arabica coffee affect the hardness of the nanofiller composite resin, but there is no significant difference in the hardness between the robusta and arabica coffee groups.Keywords: composite resin hardness, nanofiller, robusta coffee, arabica coffee Abstrak: Resin komposit nanofiller memiliki ukuran filler kecil yang dapat meningkatkan fungsinya namun fungsi tersebut dapat menurun oleh beberapa hal. Cairan asam yang dikonsumsi seperti kopi dapat menurunkan kekerasan resin komposit. Penelitian ini bertujuan untuk mengetahui pengaruh perendaman kopi robusta dan arabika terhadap kekerasan resin komposit nanofiller. Sampel resin komposit yang digunakan sebanyak 27 buah dan dilanjutkan dengan pemolesan menggunakan soflex disc selama 30 detik setiap tingkat kekasarannya. Sampel kemudian dibagi dalam tiga kelompok, yaitu kelompok kontrol dengan saliva buatan dan dua kelompok perlakuan dengan kopi robusta dan arabika. Sebanyak 27 buah resin komposit direndam ke dalam tiga kelompok tersebut selama lima hari kemudian diuji kekerasannya dengan vickers hardness tester. Saliva buatan sebagai kelompok kontrol memiliki nilai rerata kekerasan tertinggi sebesar 112,98±8,67 VHN, diikuti kopi robusta, dan nilai terendah yaitu kopi arabika. Hasil uji One Way Anova dan post hoc LSD menunjukkan terdapat perbedaan bermakna pada semua kelompok (p<0,05) kecuali pada kedua kelompok perlakuan yaitu antara kopi robusta dan arabika tidak menunjukkan adanya perbedaan kekerasan yang bermakna (p>0,05). Simpulan penelitian ini ialah kopi robusta dan arabika memengaruhi kekerasan resin komposit nanofiller namun tidak terdapat perbedaan kekerasan antara keduanya.Kata kunci: kekerasan resin komposit, nanofiller, kopi robusta, kopi arabika


2014 ◽  
Vol 3 (1) ◽  
pp. 15
Author(s):  
Rini Setyowati ◽  
Sudarsono Sudarsono ◽  
Setyowati E P

<p>“Kayu kuning” (<em>Arcangelisia flava </em>L.Merr) was used when someone has a skin problem caused by <em>Candida </em>albicans<em> </em>and <em>Trichophyton mentagrophytes</em>. Scientific based medicine on this traditional knowledge was necessary be  done. Stem powderwas extracted by  distilled water.The extract was then evaporated. Qualitative and quantitative analysis of the active substance e.g., Berberin chloride by Thin Layer Chromatography (TLC)  The antifungal activity againts <em>Candida albicans</em> and <em>Trichophyton mentagrophytes</em>were tested by using agar diffusion and microdilution methods.  The absorbance from microdilution were analized by One way ANOVA. The conclusion showed that the extract contained 1.55±0.12% w/walkaloid calculated as Berberine chloride. The inhibition zone for <em>Candida albicans </em>and <em>Trichophyton mentagrophytes </em>were 16.65±4.52 and 6.55±0.05 mm respectively. The MIC vallue for both fungi was 10 mg/mL.The MBC value for <em>Candida albicans</em> was 40 mg/mL and for <em>Trichophyton mentagrophytes </em>was 50 mg/mL. From the analysis with one-way ANOVA, shows that there are significant differences between the positive control group and the test solution with the negative control group with p=0.020 for <em>Candida albicans</em> and p=0.028 for <em>Trichophyton mentagrophytes</em> (p&lt;0.050). Post hoc Tukey analysis results showed  that both inter-group and between the concentration of the test solution to the control group did not differ significantly positive because the value of p&gt;0.050.</p>


2015 ◽  
Vol 20 (2) ◽  
pp. 156
Author(s):  
Prasetyo Adi ◽  
Ambar Puspitasari ◽  
Murni Ukhuwah I

Saliva adalah sekelompok cairan oral yang kompleks. Salah satu mikroorganisme yang menyebabkan pH saliva menjadi asam adalah Streptococcus mutans. Rebusan kelopak bunga Rossella (Habiscus sabdariffa L) mengandung flavonoid dan antosianin yang dapat mengganggu aktivitas sel dan pertumbuhan Streptococcus mutans. Tujuan penelitian ini mengetahui pengaruh konsentrasi rebusan kelopak bunga Rossella terhadap pH dan absorbansi saliva buatan yang diinduksi Streptococcus mutans secara in vitro. Penelitian ini menggunakan True Experimental Design yaitu Posttest Control Group Design. Konsentrasi yang digunakan dalam penelitian ini adalah 5%, 15%, 25%, dan 35%. Hasil penelitian menunjukkan ada perbedaan nilai pH dan absorbansi saliva buatan yang telah diinduksi Streptococcus mutans dan ditambahkan dengan rebusan kelopak bunga Rossella terhadap kelompok kontrol secara in vitro. Analisa data menggunakan uji Korelasi dan Regresi menunjukkan pengaruh sebesar 99,2% pada pemberian rebusan kelopak bunga Rossella terhadap nilai pH dan absorbansi saliva buatan. Uji One-Way ANOVA menunjukkan nilai signifikansi 0,000 (p< 0,05). Disimpulkan terdapat perbedaan nilai pH dan absorbansi saliva buatan yang signifikan antara kelompok kontrol dan kelompok perlakuan.The Effect of Rossella Flower Petals Stew to Artificial Saliva pH. Saliva is a complex oral fluids. One of the microorganisms that cause the saliva’s pH becomes acid is Streptococcus mutans. Water stew of Rossella flower petals (Habiscus sabdariffa L) contains flavonoids and anthocyanins that interfere metabolism of the cells and growth of Streptococcus mutans. The purpose of this experimental was to know the effect of water stew Rossella flower petals concentration in pH scale and absorbance score of artificial saliva which induced with Streptococcus mutans by in vitro. The method which applied here was True Experimental Design, post test control group design. The concentrations that used in this study were 5%, 15%, 25% and 35%. The results described the differences in pH scale and absorbance score of artificial saliva which induced with Streptococcus mutans between treatment group (added by water stew Rossella petals) and control group. Data analysis using correlation and regression test showed the effect of water stew of Rossella flower petals in pH scale of artificial saliva was 99.2%. The conclusion of this experimental explained the increase of pH scale after added with the water stew of Rossella flower petals, also showed a significant differences of pH scale of artificial saliva between treatment group and control group based on the One-Way ANOVA test, and the water stew of Rossella flower petals was effective to maintain pH scale of artificial saliva approach normal pH scale


2002 ◽  
Vol 16 (2) ◽  
pp. 137-143 ◽  
Author(s):  
Aline de Almeida Neves ◽  
Rodolfo de Almeida Castro ◽  
Eduardo Tavares Coutinho ◽  
Laura Guimarães Primo

The aim of this study was to investigate, in vitro, the morphological characteristics of demineralized primary enamel subjected to brushing with a dentifrice with or without fluoride. In order to do so, 32 enamel blocks were divided in 4 different groups containing 8 blocks each. They were separately immersed in artificial saliva for 15 days. The experimental groups were: C - control; E - submitted to etching with 37% phosphoric acid gel (30 s); EB - submitted to etching and brushing 3 times a day with a non-fluoridated dentifrice; EBF = submitted to etching and brushing 3 times a day with a fluoridated dentifrice. The toothbrushing force was standardized at 0.2 kgf and 15 double strokes were performed on each block. After the experimental period, the samples were prepared and examined under SEM. The control group (C) showed a smooth surface, presenting scratches caused by habitual toothbrushing. The etched samples (E) exhibited different degrees of surface disintegration, but the pattern of acid etching was predominantly the type II dissolution. The brushed surfaces were smooth, with elevations which corresponded to the exposure of Tomes’ process pits and depressions which corresponded to interrod enamel. Particles resembling calcium carbonate were found in the most protected parts of the grooves. No morphological differences were observed between brushing with fluoridated (EBF) and non-fluoridated (EB) dentifrice. The results suggest that the mechanical abrasion caused by brushing demineralized enamel with dentifrice smoothes the rough etched surface, and the presence of fluoride does not cause morphological modifications in this pattern.


Author(s):  
Ugonna D. Nwankpa ◽  
Henry N. Ogbonna ◽  
Chinedu E. Udekwu ◽  
Chibuzo V. Nwokafor

Aim: To evaluate the nephrotoxic and hepatotoxic potentials of artesunate in humans. Place and Duration of Study: Department of Biochemistry, Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria, between November and December 2019. Methodology: 70 blood samples were collected from 35 normal individuals (control group), and 35 malaria patients treated with parenteral artesunate (treatment group). These were analyzed for biochemical parameters, including blood urea nitrogen (BUN), creatinine, total bilirubin, aspartate transaminase (AST), alanine transaminase (ALT), and alkaline phosphatase (ALP). The treatment group was further regrouped according to gender (19 males and 16 females), age (20 patients aged 20-29 and 15 patients aged 30-40 years) and duration of treatment (29 patients on 3rd or 4th day and 6 patients on 5th or 6th day of treatment). Biochemical tests were carried out using standard Randox test kits. One-way ANOVA was done on the parameters using statistical package for social sciences (SPSS), and comparisons were made. Results: Compared to control group, the treatment group showed significant increases (p<0.05) in BUN (15.89+1.30 against 11.69+0.62), Creatinine (0.96+0.62 against 0.82+0.03) and AST (22.14+2.45 against 16.66+0.85), a non-significant increase (p 0.05) in ALT (26.57+3.18 against 21.66+2.56) and ALP (85.31+4.06 against 77.54+3.09) and a non-significant decrease (p 0.05) in total bilirubin (0.59+0.06 against 0.65+0.06). However, all parameters examined were within the normal ranges. There was no significant relationship found in any parameter in a comparison of gender, age and duration of treatment. Conclusion: Since all parameters examined were in the normal ranges, administration of artesunate in the recommended dosage and the right duration may not have any significant toxic effect on the kidney and liver. However, further studies may be necessary to ascertain if the observed elevations could be attributed wholly to artesunate or other medications taken by the malaria patients.


2017 ◽  
Vol 18 (7) ◽  
pp. 583-586 ◽  
Author(s):  
En HR Haghgou ◽  
Roza Haghgoo ◽  
Mohamad R Roholahi ◽  
Zahra Ghorbani

ABSTRACT Aim This study aims to investigate the effect of casein phosphopeptide-amorphous calcium phosphate and three calcium phosphate (CPP-ACP and TCP) on increasing the microhardness of human enamel after induction of erosion. Materials and methods A total of 26 healthy human-impacted third molar teeth were chosen, and their hardness measured using a microhardness testing machine. The samples were immersed in Coca Cola (pH = 4.7) for 8 minutes. Then, microhardness was measured again, and these samples were randomly divided into four groups (two control groups and two experimental groups). (1) Negative control group: Artificial saliva was used for 10 minutes, (2) positive control group: Fluoride gel was used for 10 minutes, (3) β-TCP group: TCP was used for 10 minutes, (4) CCP-ACP group: CCP-ACP was used for 10 minutes. The final microhardness of those samples was measured, and the changes in microhardness of teeth within group and between groups were analyzed using the paired and analysis of variance tests respectively. Results were considered statistically significant at a level of p < 0.05. Results No significant difference was observed in microhardness between CPP-ACP group and TCP group (p = 0.368) during the time microhardness significantly dropped after soaking in soda. Conclusion Casein phosphopeptide-amorphous calcium phosphate and TCP increased the microhardness of teeth. The increase in hardness in the TCP group was higher than in the CPP-ACP group, but this difference was not significant (p = 0.36). Clinical significance Casein phosphopeptide-amorphous calcium phosphate and TCP can affect the remineralization of erosive lesions. How to cite this article Haghgou EHR, Haghgoo R, Roholahi MR, Ghorbani Z. Effect of Casein Phosphopeptide-Amorphous Calcium Phosphate and Three Calcium Phosphate on Enamel Microhardness. J Contemp Dent Pract 2017;18(7):583-586.


2016 ◽  
Vol 2 (3) ◽  
pp. 141 ◽  
Author(s):  
Miftah Wiryani ◽  
Billy Sujatmiko ◽  
Rini Bikarindrasari

The effect of application time of CPP-ACPF on enamel hardness. Remineralization process can increase the hardness of enamel due to demineralization process. CPP-ACPF is a material used for enhancing remineralization. However, the application time of CPP-ACPF remain controversial among previous studies. This study was aimed to investigate the effect of various application times of CPP-ACPF on enamel hardness. Thirty premolar teeth were mounted on self cure acrylic resin, and were divided into 5 groups. Demineralization process was performed, and enamel hardness (pre-est) was measured by Vickers Hardness Tester. Remineralization was performed using CPP-ACPF in various application times: 3, 15, 30, 60 minutes, and the control group was only immersed in artificial saliva for 60 minutes, then enamel hardness was measured (posttest). Data were analyzed using paired t-test, one-way ANOVA, and post-hoc Bonferroni. The result of paired t-test showed that all the groups, except the control group, have an increasing enamel hardness that was statistically significant. One-way ANOVA results showed no statistically significant difference among the groups at pretest, but one-way ANOVA results showed statistically significant difference at posttest. Post hoc Bonferroni showed that the significantly difference at posttest occurred between all the treatment groups against the control group, but there were no significant differences between the 3 minutes group to 15 minutes group, between 15 minutes group to 30 minutes group, and between 30 minutes group to 60 minutes group. It was concluded that various application times of CPP-ACPF had an effect on increasing enamel hardness. ABSTRAKProses remineralisasi dapat meningkatkan kekerasan email yang menurun akibat demineralisasi. Bahan remineralisasi yang ideal adalah CPP-ACPF. Terdapat perbedaan lama aplikasi CPP-ACPF dalam berbagai penelitian, selain itu total lama aplikasi yang dibutuhkan CPP-ACPF dalam mekanisme remineralisasi belum diketahui. Penelitian ini dilakukan untuk mengetahui pengaruh lama aplikasi CPP-ACPF terhadap kekerasan email. Tiga puluh mahkota gigi premolar yang ditanam dalam resin akrilik self cure dibagi menjadi lima kelompok, kemudian dilakukan proses demineralisasi. Kekerasan email kemudian diukur menggunakan alat Vickers Hardness Tester. Proses remineralisasi menggunakan CPP-ACPF dilakukan pada masing-masing kelompok dalam berbagai lama aplikasi yaitu 3 menit, 15 menit, 30 menit, 60 menit, serta perendaman dalam saliva buatan selama 60 menit (kontrol). Kekerasan email kemudian diukur kembali (posttest). Data diuji secara statistik menggunakan t-test berpasangan, one-way ANOVA dan post hoc Bonferroni. Hasil paired t-test menunjukkan bahwa seluruh kelompok, kecuali kelompok kontrol, mengalami peningkatan rata-rata kekerasan email secara signifikan. Hasil uji one-way ANOVA pada pretest menunjukkan tidak ada perbedaan kekerasan email yang signifikan. Hasil uji one-way ANOVA pada posttest menunjukkan terdapat perbedaan kekerasan email yang signifikan. Hasil uji post Hoc Bonferroni menunjukkan bahwa terdapat perbedaan kekerasan email yang signifikan pada seluruh kelompok perlakuan terhadap kelompok kontrol, tetapi perbedaan kekerasan email antara kelompok lama aplikasi 3 menit dengan 15 menit, antara lama aplikasi 15 menit dengan 30 menit, serta antara lama aplikasi 30 menit dengan 60 menit tidak menunjukkan perbedaan kekerasan email yang signikan. Kesimpulan penelitian ini adalah berbagai lama aplikasi CPP-ACPF berpengaruh terhadap peningkatan kekerasan email.


2015 ◽  
Vol 6 (3) ◽  
pp. 174-177
Author(s):  
S Srilatha ◽  
Zeeshan Hasanali Ladhani ◽  
Sayli Dargad ◽  
Vighnesh Dixit

ABSTRACT Background To evaluate microhardness of enamel surface after microabrasion of enamel using casein phosphopeptideamorphous calcium phosphate (CPP-ACP) and casein phosphopeptide-amorphous calcium phosphate with fluoride (CPP-ACPF). Materials and methods Ten freshly extracted anterior teeth were selected. Teeth were treated with abrasive slurry. This prepared slurry was applied to the labial aspects of teeth, using a rotating rubber cup, for 50 seconds. Each tooth was divided into four parts and treated accordingly with the topical application of the demineralizing agent stored in artificial saliva. The teeth were divided into four groups, as follows: – Group A: Control group – Group B: Microabrasion done but not treated – Group C: Microabrasion done and CPP-ACP paste applied – Group D: Microabrasion done and CPP-ACPF (i.e. with fluoride) applied. The microhardness was evaluated using the Vicker's microhardness test. Results Microhardness values were highest for the control group followed by group D, group C and finally group B. Microabraded group of teeth with no topical application showed least microhardness values of all. Conclusion The CPP-ACPF increased the microabrasion significantly as compared to CPP-ACP and microabrasion group. How to cite this article Ladhani ZH, Dargad S, Dixit V, Srilatha S, Hegde V. Effect of Application of Remineralizing Agents on the Microhardness of Microabraded Teeth. World J Dent 2015;6(3):174-177.


2015 ◽  
Vol 12 (1) ◽  
pp. 16-20 ◽  
Author(s):  
N Sathe ◽  
RVS Raju Chakradhar ◽  
V Chandrasekhar

Background Caries process is not a static one, but is dynamic with interspersed periods of demineralization and remineralization of enamel, intimately related and occurs episodically based upon the presence of cariogenic bacteria in dental plaque and the availability of refined carbohydrates for fermentation to organic acids.Objectives Early enamel caries could be reversed with avoidance of frank cavitation. The main objective of this study is to check whether enamel demineralization can be prevented by using the remineralizing agents.Method Forty freshly extracted human central incisors were selected and stored in saline at normal temperature. A window of 3 X 3mm enamel was created and all the specimens were then randomly divided into 4 groups of 10 each. Group I- teeth received no treatment, Group II- teeth treated with Acidulated Phosphate Flouride gel, Group III- teeth treated with Tooth Mousse Plus, Group IV- teeth treated with Remin+. Samples in all the groups were kept in artificial saliva for 24 hours and subjected to modified Ten Cate’s solution at an acidic pH of 3.5 for 10 days. The samples were sectioned and subjected to SEM evaluation.Result Scanning Electron Microscope(SEM) images showed decrease in pore volume of the enamel in all the treatment groups compared to the control group indicating increase in resistance to demineralization in acidic pH.Conclusion The three groups of remineralizing agents, Acidulated Phosphate Fluoride gel, Tooth Mousse Plus and Remin + showed significant increase in fluoride content and negligible increase in calcium content indicating there is remineraliztion.Kathmandu University Medical Journal Vol.12(1) 2014: 16-20


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