The Mechanism Behind the Promotive Effect of Foliar Application of 5-Aminolevulinic Acid (ALA) in Tomato Plants Under Salt Stress

The effect of foliar ALA application on the internal ALA concentration in tomato plants grown in soil containing high levels of NaCl was investigated. Six week old plants were treated with 100, 50, and 25 mmol/L NaCl on a weekly basis and they were simultaneously treated with 5-ALA at a concentration of 5% by foliar spray. The effect of foliar ALA application on plant growth, chlorophyll contents and internal ALA concentration was studied. The internal ALA shoot concentrations ranged between 27.50±2.12 and 34.35±1.48 µg g-1 dry weight with no significant difference (p<0.05) recorded between plants treated with NaCl alone and those treated with both NaCl and ALA. The concentrations of chlorophyll a and b were elevated only in tomato plants treated with NaCl and ALA, whereas their levels decreased in plants treated with NaCl only. An adverse significant effect (p<0.05) of salinity stress was recorded on plants length, number of leaves, shoot and root fresh and dry weight. However, no significant difference (p<0.05) was observed in plants treated with NaCl alone with those treated with NaCl plus ALA in comparison to the control. The results of the present study suggested that foliar ALA treatment had no effect on the Na and Cl uptake, the internal ALA concentration, and had no role in adverting the effects of salinity on plant growth. The present study has proven that foliar ALA is directly used by the plant for the synthesis of chlorophyll in order to increase the photosynthetic rate and thus to help tomato plants to survive the salinity stress.

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Effect of Foliar Application of Growth Regulators on Vegetative and Physiological Growth Parameters in Ajowan (Trachyspermum ammi L. Sprague)

Current Journal of Applied Science and Technology ◽

10.9734/cjast/2020/v39i2930962 ◽

2020 ◽

pp. 100-109

Author(s):

Y. Rajasekhara Reddy ◽

G. Ramanandam ◽

P. Subbaramamma ◽

A. V. D. Dorajeerao

Keyword(s):

Leaf Area ◽

Foliar Application ◽

Growth Parameters ◽

Block Design ◽

Foliar Spray ◽

Dry Weight ◽

Area Index ◽

Chlorophyll Contents ◽

Trachyspermum Ammi ◽

Number Of Leaves

A field experiment was carried out during rabi season of 2018-2019, at college farm, College of Horticulture, Dr. Y.S.R. Horticultural University, Venkataramannagudem, West Godavari District, Andhra Pradesh. The experiment was laidout in a Randomised Block Design with eleven treatments (viz., T1- NAA @ 50 ppm, T2-NAA @ 100 ppm, T3-GA3 @ 50 ppm, T4-GA3 @ 100 ppm, T5-Thiourea @ 250 ppm, T6-Thiourea @ 500 ppm, T7-28-Homobrassinolide @ 0.1 ppm, T8-28-Homobrassinolide @ 0.2 ppm, T9-Triacontinol @ 2.5 ppm, T10-Triacontinol @ 5 ppm, T11-(Control) Water spray) and three replications. The treatments were imposed at 30 and 45 DAT in the form of foliar spray. Foliar application of GA3@ 100 ppm (T4) had recorded the maximum plant height (108.20 cm), leaf area (9.53 cm2) and leaf area index (0.74). Foliar application of thiourea @ 250 ppm (T5) had recorded the maximum values with respect to number of primary branches (15.03 plant-1), number of secondary branches (83.40 plant-1), plant spread (1793 cm2 plant-1), fresh weight (376.29 g plant-1), dry weight (103.54 g plant-1) and number of leaves plant-1((298.8). The same treatment (T5) had recorded the highest values with respect to crop growth rate (1.44 gm-2d-1), chlorophyll-a (1.40 mg g-1), chlorophyll-b (0.076 mg g-1) and total chlorophyll contents (1.48 mg g-1) in the leaves.

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Efektivitas Frekuensi dan Volume Penyemprotan Daun dengan Agens Hayati Filosfer dalam Meningkatkan Pertumbuhan Tanaman dan Hasil Padi

Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) ◽

10.24831/jai.v44i2.13480 ◽

2016 ◽

Vol 44 (2) ◽

pp. 141 ◽

Cited By ~ 1

Author(s):

Samsi Abdul Khodar ◽

Satriyas Ilyas ◽

Dan Candra Budiman

Keyword(s):

Foliar Application ◽

Control Volume ◽

Block Design ◽

Foliar Spray ◽

Dry Weight ◽

Biological Agents ◽

Xanthomonas Oryzae ◽

Rice Seed ◽

Significant Difference ◽

Two Factors

ABSTRACT Increased of plant productivity in field determined by the use of quality seed from improved variety. The purpose of this research was to study the effect of frequency and volume of phyllosphere biological agents by foliar spray on rice plant infected by Xanthomonas oryzae pv. oryzae (Xoo) in improving plant growth, production of rice seed, and controlling bacterial leaf blight (BLB). This research was conducted at Seed Health Laboratory, Department of Agronomy and Horticulture, Bogor Agricultural University, and Muara Experimental Field, Rice Research Institute, March through September 2014. The experiment was conducted using a randomized complete block design with two factors, and replicates as blocks. The first factor was five levels of frequency of spraying with biological agents: P0 = control, P1 = Xoo seed inoculated then invigorated with biomatriconditioning, P2 = Xoo inoculated seed, then biomatriconditioned, and the plants were sprayed with phyllosphere biological agents F112 once, P3 = as P2, and the plants were sprayed with F112 twice, and P4 = as P2, and the plants were sprayed with F112 three times. The second factor was three levels of spray volume: i.e., 300, 400, and 500 L ha-1. Result of this experiment revealed that biomatriconditioning with rhizobacteria P. diminuta A6 and B. subtilis 5/B improved field emergence and seedling dry weight. Seeds applied with biomatriconditioning then followed by foliar spray with phyllosphere bacteria F112 at 40, 60, and 80 days after transplanting effectively reduced severity of BLB, increased plant dry weight and seed yield. All treatments increased yield as compared to untreated control. Volume of foliar application showed no significant difference, therefore, the lowest volume of 300 L ha-1 was recommended. Keywords: Bacillus subtilis 5/B, biomatriconditioning, Pseudomonas diminuta A6, Xanthomonas oryzae pv. oryzae

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Effects of 6-Benzyladenine, γ-Aminobutyric Acid, and Nitric Oxide on Plant Growth, Photochemical Efficiency, and Ion Accumulation of Perennial Ryegrass Cultivars to Salinity Stress

HortScience ◽

10.21273/hortsci14067-19 ◽

2019 ◽

Vol 54 (8) ◽

pp. 1418-1422 ◽

Cited By ~ 1

Author(s):

Zhongjie Ji ◽

James J. Camberato ◽

Cankui Zhang ◽

Yiwei Jiang

Keyword(s):

Nitric Oxide ◽

Plant Growth ◽

Salinity Stress ◽

Perennial Ryegrass ◽

Photochemical Efficiency ◽

Foliar Spray ◽

Leaf Tissue ◽

Dry Weight ◽

Aminobutyric Acid ◽

Higher Plant

Plant growth regulators (PGRs) can mediate plant response to salinity stress. Perennial ryegrass (Lolium perenne) cultivars of BrightStar SLT, Catalina, Inspire, and SR4660ST were exposed to 0, 100, or 200 mm NaCl for 14 d. 6-benzyladenine (6-BA, 10 µm), γ-aminobutyric acid (GABA, 500 µm), nitric oxide (NO, 200 µm), and H2O were applied to the foliage every day for 3 days before stress and then every 2 days during salinity stress. Averaged across the four cultivars, a foliar spray of NO increased leaf fresh weight (FW) and dry weight (DW) at 0 mm NaCl, whereas application of 6-BA increased DW and GABA reduced Na+ concentration at 100 mm NaCl, compared with H2O application. Plants treated with 6-BA, GABA, and NO had less chlorotic and necrotic leaf tissue than plants treated with H2O at 200 mm NaCl. Spray of 6-BA and NO increased FW and DW, but application of all three PGRs maintained higher leaf photochemical efficiency and lower leaf Na+ concentration compared with H2O treatment at 200 mm NaCl. Across salinity and PGR treatments, ‘Catalina’ exhibited higher plant height than the ‘Inspire’ and SR4660ST, and SR4660ST had relatively higher Na+ concentration than ‘Catalina’ but not ‘BrightStar SLT’ and ‘Inspire’. The results demonstrate that 6-BA, GABA, and NO ameliorated salinity tolerance of perennial ryegrass by improving growth and photochemical efficiency or reducing Na+ accumulation.

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Halotolerant Microbial Consortia for Sustainable Mitigation of Salinity Stress, Growth Promotion, and Mineral Uptake in Tomato Plants and Soil Nutrient Enrichment

Sustainability ◽

10.3390/su13158369 ◽

2021 ◽

Vol 13 (15) ◽

pp. 8369

Author(s):

Chintan Kapadia ◽

R. Z. Sayyed ◽

Hesham Ali El Enshasy ◽

Harihar Vaidya ◽

Deepshika Sharma ◽

...

Keyword(s):

Salt Stress ◽

Salinity Stress ◽

Dry Weight ◽

Stress Factors ◽

Microbial Consortia ◽

Soil Conditions ◽

Mineral Uptake ◽

Shoot Height ◽

Tomato Plants ◽

Secondary Roots

Salinity significantly impacts the growth, development, and reproductive biology of various crops such as vegetables. The cultivable area is reduced due to the accumulation of salts and chemicals currently in use and is not amenable to a large extent to avoid such abiotic stress factors. The addition of microbes enriches the soil without any adverse effects. The effects of microbial consortia comprising Bacillus sp., Delftia sp., Enterobacter sp., Achromobacter sp., was evaluated on the growth and mineral uptake in tomatoes (Solanum Lycopersicum L.) under salt stress and normal soil conditions. Salinity treatments comprising Ec 0, 2, 5, and 8 dS/m were established by mixing soil with seawater until the desired Ec was achieved. The seedlings were transplanted in the pots of the respective pH and were inoculated with microbial consortia. After sufficient growth, these seedlings were transplanted in soil seedling trays. The measurement of soil minerals such as Na, K, Ca, Mg, Cu, Mn, and pH and the Ec were evaluated and compared with the control 0 days, 15 days, and 35 days after inoculation. The results were found to be non-significant for the soil parameters. In the uninoculated seedlings’ (control) seedling trays, salt treatment significantly affected leaf, shoot, root dry weight, shoot height, number of secondary roots, chlorophyll, and mineral contents. While bacterized seedlings sown under saline soil significantly increased leaf (105.17%), shoot (105.62%), root (109.06%) dry weight, leaf number (75.68%), shoot length (92.95%), root length (146.14%), secondary roots (91.23%), and chlorophyll content (−61.49%) as compared to the control (without consortia). The Na and K intake were higher even in the presence of the microbes, but the beneficial effect of the microbe helps plants sustain in the saline environment. The inoculation of microbial consortia produced more secondary roots, which accumulate more minerals and transport substances to the different parts of the plant; thus, it produced higher biomass and growth. Results of the present study revealed that the treatment with microbial consortia could alleviate the deleterious effects of salinity stress and improve the growth of tomato plants under salinity stress. Microbial consortia appear to be the best alternative and cost-effective and sustainable approach for managing soil salinity and improving plant growth under salt stress conditions.

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Acetyl salicylic acid alleviates chilling-induced damage in muskmelon seedlings

Canadian Journal of Plant Science ◽

10.4141/cjps06035 ◽

2007 ◽

Vol 87 (3) ◽

pp. 581-585 ◽

Cited By ~ 11

Author(s):

Ahmet Korkmaz ◽

Murat Uzunlu ◽

Ali Riza Demirkiran

Keyword(s):

Salicylic Acid ◽

Cucumis Melo ◽

Chilling Stress ◽

Foliar Spray ◽

Dry Weight ◽

Acetyl Salicylic Acid ◽

Signal Molecule ◽

Stress Indicator ◽

Shoot Dry Weight ◽

Significant Difference

Salicylic acid (SA) is a common plant-produced signal molecule that is responsible for inducing tolerance to a number of biotic and abiotic stresses. An experiment was, therefore, conducted to test whether acetyl salicylic acid (ASA) application at various concentrations through seed immersion or foliar spray would protect muskmelon [Cucumis melo L. (Reticulatus Group)] seedlings subjected to chilling stress. Twenty-one-day-old plants pre-treated with ASA (0, 0.1, 0.25, 0.50 or 1.0 mM) were subjected to chilling stress for 72 h at 3 ± 0.5°C. ASA, applied either through seed immersion or foliar spray, was effective within the range of 0.1 to 1 mM in inducing tolerance to chilling stress in muskmelon seedlings; however, there was no significant difference between application methods. ASA significantly and curvilinearly affected all seedling growth and stress indicator variables tested except shoot dry weight. The best protection was obtained from seedlings pre-treated with 0.5 mM ASA. The highest ASA concentration used was slightly less effective in providing chilling stress protection. Even though both methods provided similar means of protection, due to its simplicity and practicality, immersion of muskmelon seeds prior to sowing in 0.5 mM ASA would be a more desirable method to induce tolerance to chilling stress. Key words: Cucumis melo, aspirin, chilling stress tolerance, gas exchange, electrolyte leakage

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Effect of different levels of humic acids on the nutrient content, plant growth, and soil properties under conditions of salinity

Soil and Water Research ◽

10.17221/4/2010-swr ◽

2011 ◽

Vol 6 (No. 1) ◽

pp. 21-29 ◽

Cited By ~ 65

Author(s):

H. Khaled ◽

H.A. Fawy

Keyword(s):

Plant Growth ◽

Humic Substances ◽

Humic Acids ◽

Foliar Application ◽

N Uptake ◽

Seedling Emergence ◽

Dry Weight ◽

Nutrient Content ◽

Nutrient Elements ◽

A Minor

In this study, the effects were investigated of salinity, foliar and soil applications of humic substances on the growth and mineral nutrients uptake of Corn (Hagein, Fardy10), and the comparison was carried out of the soil and foliar applications of humic acid treatments at different NaCl levels. Soil organic contents are one of the most important parts that they directly affect the soil fertility and textures with their complex and heterogenous structures although they occupy a minor percentage of the soil weight. Humic acids are an important soil component that can improve nutrient availability and impact on other important chemical, biological, and physical properties of soils. The effects of foliar and soil applications of humic substances on the plant growth and some nutrient elements uptake of Corn (Hagein, Fardy10) grown at various salt concentrations were examined. Sodium chloride was added to the soil to obtain 20 and 60mM saline conditions. Solid humus was applied to the soil one month before planting and liquid humic acids were sprayed on the leaves twice on 20th and 40th day after seedling emergence. The application doses of solid humus were 0, 2 and 4 g/kg and those of liquid humic acids were 0, 0.1 and 0.2%. Salinity negatively affected the growth of corn; it also decreased the dry weight and the uptake of nutrient elements except for Na and Mn. Soil application of humus increased the N uptake of corn while foliar application of humic acids increased the uptake of P, K, Mg,Na,Cu and Zn. Although the effect of interaction between salt and soil humus application was found statistically significant, the interaction effect between salt and foliar humic acids treatment was not found significant. Under salt stress, the first doses of both soil and foliar application of humic substances increased the uptake of nutrients.

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Improvement of antioxidant activities and yield of spring maize through seed priming and foliar application of plant growth regulators under heat stress conditions

Semina Ciências Agrárias ◽

10.5433/1679-0359.2017v38n1p47 ◽

2017 ◽

Vol 38 (1) ◽

pp. 47 ◽

Cited By ~ 2

Author(s):

Ijaz Ahmad ◽

Shehzad Maqsood Ahmed Basra ◽

Muhammad Akram ◽

Allah Wasaya ◽

Muhammad Ansar ◽

...

Keyword(s):

Heat Stress ◽

Grain Yield ◽

Foliar Application ◽

Antioxidant Activities ◽

Foliar Spray ◽

Seed Priming ◽

Chlorophyll Contents ◽

Exogenous Application ◽

Hybrid Maize

Heat stress during reproductive and grain filling phases adversely affects the growth of cereals through reduction in grain’s number and size. However, exogenous application of antioxidants, plant growth regulators and osmoprotectants may be helpful to minimize these heat induced yield losses in cereals. This two year study was conducted to evaluate the role of exogenous application of ascorbic acid (AsA), salicylic acid (SA) and hydrogen peroxide (H2O2) applied through seed priming or foliar spray on biochemical, physiological, morphological and yield related traits, grain yield and quality of late spring sown hybrid maize. The experiment was conducted in the spring season of 2007 and 2008. We observed that application of AsA, SA and H2O2 applied through seed priming or foliar spray improved the physiological, biochemical, morphological and yield related traits, grain yield and grain quality of late spring sown maize in both years. In both years, we observed higher superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) activity in the plants where AsA, SA and H2O2were applied through seed priming or foliar spray than control. Membrane stability index (MSI), relative water contents (RWC), chlorophyll contents, grain yield and grain oil contents were also improved by exogenous application of AsA, SA and H2O2 in both years. Seed priming of AsA, SA and H2O2was equally effective as the foliar application. In conclusion, seed priming with AsA, SA and H2O2 may be opted to lessen the heat induced yield losses in late sown spring hybrid maize. Heat tolerance induced by ASA, SA and H2O2 may be attributed to increase in antioxidant activities and MSI which maintained RWC and chlorophyll contents in maize resulting in better grain yield in heat stress conditions.

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Impact of Exogenous Plant Growth Regulators on Physiological Traits, Tendril and Flowering in Horsegram (Macrotyloma uniflorum Lam) under Kharif Season

Legume Research - An International Journal ◽

10.18805/lr-4771 ◽

2021 ◽

Author(s):

R. Sivakumar

Keyword(s):

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Foliar Application ◽

Leaf Temperature ◽

Physiological Traits ◽

Mepiquat Chloride ◽

Kharif Season ◽

Significant Difference ◽

The Impact

Background: Horsegram is an important pulse crop grown as Rabi crop in Tamil Nadu. Kharif sown crop could not flowers may be because of more number of tendrils produced as compare to Rabi sown crop. Hence, an attempt was made to reduce the number of tendrils and induce flowering in horsegram during Kharif season by plant growth regulators. Methods: A field experiment was conducted to study the impact of plant growth regulators viz., salicylic acid (100 ppm), chlormequat chloride (CCC - 250 ppm), mepiquat chloride (250 ppm), tri iodo benzoic acid (TIBA - 200 ppm) and nitrobenzene (0.2%) on physiological traits and tendril growth associated with flowering in horsegram during Kharif. Different treatments were applied through foliar application at 25 Days after sowing. Result: Among the PGRs, foliar application of TIBA registered the lowest number of tendrils (2.3) followed by CCC (3.0) compared to other treatments. Higher root length of 16.8 cm and RWC of 82.3% was found in CCC treatment. CCC also registered the highest photosynthetic rate (27.15 µmol m-2 s-1), transpiration rate (18.06 mmol m-2 s-1) and lowest leaf temperature (26°C) compared to other treatments. The highest soluble protein content of 13.1 mg g-1 was also estimated in CCC treatment followed by mepiquat chloride (12.51 mg g-1). Sucrose phosphate synthase (SPS) activity did not show any significant difference between the treatments. Number of flowers buds formed per plant was zero and hence the flowering did not take place in any treatments. Among the plant growth regulators used, TIBA and CCC registered its positive action on reduced the number of tendrils and leaf temperature, but not enough to induce flowering under Kharif season.

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Relief Role of Lysine Chelated Zinc (Zn) on 6-Week-Old Maize Plants under Tannery Wastewater Irrigation Stress

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph17145161 ◽

2020 ◽

Vol 17 (14) ◽

pp. 5161

Author(s):

Rehan Ahmad ◽

Wajid Ishaque ◽

Mumtaz Khan ◽

Umair Ashraf ◽

Muhammad Atif Riaz ◽

...

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Plant Growth ◽

Foliar Application ◽

Antioxidant Activities ◽

Foliar Spray ◽

Tannery Wastewater ◽

Organic Load ◽

Maize Plants

Tannery wastewater mainly comes from leather industries. It has high organic load, high salinity, and many other pollutants, including chromium (Cr). Tannery wastewater is generally used for crop irrigation in some areas of Pakistan and worldwide, due to the low availability of good quality of irrigation water. As tannery wastewater has many nutrients in it, its lower concentration benefits the plant growth, but at a higher concentration, it damages the plants. Chromium in tannery wastewater accumulates in plants, and causes stress at physiological and biochemical levels. In recent times, the role of micronutrient-amino acid chelated compounds has been found to be helpful in reducing abiotic stress in plants. In our present study, we used lysine chelated zinc (Zn-lys) as foliar application on maize (Zea mays L.), growing in different concentrations of tannery wastewater. Zinc (Zn) is required by plants for growth, and lysine is an essential amino acid. Maize plants were grown in tannery wastewater in four concentrations (0, 25%, 50%, and 100%) and Zn-lys was applied as a foliar spray in three concentrations (0 mM, 12.5 mM, and 25 mM) during plant growth. Plants were cautiously harvested right after 6 weeks of treatment. Foliar spray of Zn-lys on maize increased the biomass and improved the plant growth. Photosynthetic pigments such as total chlorophyll, chlorophyll a, chlorophyll b and contents of carotenoids also increased with Zn-lys application. In contrast to control plants, the hydrogen peroxide (H2O2) contents were increased up to 12%, 50%, and 68% in leaves, as well as 16%, 51% and 89% in roots at 25%, 50%, and 100% tannery water application, respectively, without Zn-lys treatments. Zn-lys significantly reduced the damages caused by oxidative stress in maize plant by decreasing the overproduction of H2O2 and malondialdehyde (MDA) in maize that were produced, due to the application of high amount of tannery wastewater alone. The total free amino acids and soluble protein decreased by 10%, 31% and 64% and 18%, 61% and 122% at 25%, 50% and 100% tannery water treatment. Zn-lys application increased the amino acids production and antioxidant activities in maize plants. Zn contents increased, and Cr contents decreased, in different parts of plants with Zn-lys application. Overall, a high concentration of tannery wastewater adversely affected the plant growth, but the supplementation of Zn-lys assertively affected the plant growth and enhanced the nutritional quality, by enhancing Zn and decreasing Cr levels in plants simultaneously irrigated with tannery wastewater.

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Improving Tolerance of Faba Bean during Early Growth Stages to Salinity through Micronutrients Foliar Spray

Notulae Scientia Biologicae ◽

10.15835/nsb223701 ◽

2010 ◽

Vol 2 (2) ◽

pp. 98-102 ◽

Cited By ~ 7

Author(s):

Mohamed M. EL FOULY ◽

Zeinab M. MOBARAK ◽

Zeinab A. SALAMA

Keyword(s):

Faba Bean ◽

Irrigation Water ◽

Foliar Application ◽

Nutrient Balance ◽

Foliar Spray ◽

Dry Weight ◽

Growth Stages ◽

Plant Tolerance ◽

Negative Effect ◽

Tolerance To Salinity

Salinity, either of soil or of irrigation water, causes disturbances in plant growth and nutrient balance. Previous work indicates that applying nutrients by foliar application increases tolerance to salinity. A pot experiment with three replicates was carried out in the green house of NRC, Cairo, Egypt, to study the effect of micronutrients foliar application on salt tolerance of faba bean. Two concentrations of a micronutrient compound (0.1% and 0.15%) were sprayed in two different treatments prior to or after the salinity treatments. Levels of NaCl (0.00-1000-2000-5000 ppm) were supplied to irrigation water. Results indicated that 2000 and 5000 ppm NaCl inhibited growth and nutrient uptake. Spraying micronutrients could restore the negative effect of salinity on dry weight and nutrients uptake, when sprayed either before or after the salinity treatments. It is suggested that micronutrient foliar sprays could be used to improve plant tolerance to salinity.

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