scholarly journals PENGARUH BERBAGAI EKSTRAK AKAR DALAM STIMULASI INFEKSI CENDAWAN MIKORIZA ARBUSKULA (CMA) PADA Tithonia diversifolia

Jurnal Solum ◽  
2012 ◽  
Vol 9 (1) ◽  
pp. 36
Author(s):  
Agustian Agustian ◽  
Arwie Delfi ◽  
Lusi Maira

In the first stage of arbuscular-mycorrhiza (AMF) fungi development on plant roots showed that root exudates, is a substance that determines the success of the establishment of mycorrhizal symbiosis. This secondary metabolites produced is intended to keep mold growth during the phase preinfection. Therefore, it is interesting to know whether to add the root extract of the plant could enhance CMA fungal infection in its host plant. The purpose of this study was to determine the type of root extract can stimulate CMA infection at the root and its influence on the growth of Tithonia diversifolia. This experiment used Completely Randomized Design (CRD) with 4 treatments and 3 replications, and continued with advanced test DNMRT at level 5%. The treatments assayed were: control, Tris-HCl buffer pH 7 (A), root extract of weed Imperata cylindrica (B); root extract of taro (C), and root extract of cassava (D). Base on the results obtained we concluded that the extract of the roots of weeds, taro and cassava does not give effect to the increased growth of Tithonia. However, the roots extract stimulated and had influenced on the percentage and intensity of AMF infection at the root of Tithonia and the number of spores obtained in the rhizosphere of Tithonia. In this case the root extract of taro showed the best stimulatory effect of infection with the percentage and intensity of infection are 99.3% and 30.59% consecutively with the number of spores found reach 113 spores/ 100 g soil.Key words: Stimulation, arbuscular-mycorrhiza, root extract, Tithonia diversifolia

Author(s):  
Rinawati Rinawati ◽  
E Suharyanto ◽  
Nastiti Wijayanti

Tithonia diversifolia is a plant that has the potential to reduce blood glucose levels that is crucial in Diabetes Mellitus therapy. This study aims to determine the active compounds contained in the extract of T. diversifolia leaves decoction, the effect of decoction extract on the reduction of blood glucose, and the most effective decoction extract to reduce blood glucose levels as well. The study used a Completely Randomized Design (CRD) with the research treatment groups, namely: Kn = normal control (normal mice and not given leaves decoction extract), Ka = STZ control (DM mice, which are normal mice induced by 65mg/Kg of STZ), Kp = treatment control (normal mice given leaf decoction extract) , P1 = DM + mice were given young leaf decoction extract, P2 = DM + mice were given a mixture of young leaf and adult leaf decoction extract, P3 = DM + mice were given adult leaf decoction extract. The leaves used for decoction were 1-6 leaves from the shoots. The analysis of T. diversifolia leaves decoction compounds is using the visible spectrophotometers for tannins, phenols and flavonoids and GC-MS for terpenoid analysis. The result found that Tithonia diversifolia leaves decoction extracts contain tannins, flavonoids and phenols, while terpenoids are not detected. The leaves decoction extracts of T. diversifolia influenced the decreases in blood glucose levels of DM mice, especially the decoction extract of adult leaves which reduced blood glucose levels to 71.16%.


Weed Science ◽  
1973 ◽  
Vol 21 (4) ◽  
pp. 330-335 ◽  
Author(s):  
Allan S. Hamill ◽  
Donald Penner

The combination of the herbicide 2-chloro-2′,6′ diethyl-N-(methoxymethyl) acetanilide (alachlor) with the insecticide 2,2-dimethyl-2,3-dihydrobenzofuranyl-7-N-methyl-carbamate (carbofuran) interacted synergistically to reduce barley [Hordeum vulgareL. ‘Larker’] but not corn [Zea maysL. ‘Michigan 400’] growth. Alachlor alone increased the respiration rate of barley. Accumulation of14C from14C-alachlor was greater in the plant roots than shoots. The increased accumulation of alachlor in the roots was accentuated by a reduced rate of alachlor metabolism. The basis for the observed interaction appeared to be greater alachlor uptake by barley plants grown from seed treated with carbofuran. A statistical procedure for pesticide combinations in experiments with a completely randomized design was developed for the calculation of interaction significance.


2019 ◽  
Vol 11 (2) ◽  
pp. 99-106
Author(s):  
Muhammad Rasyid Ridha ◽  
Abdullah Fadilly ◽  
Budi Hairani ◽  
Wulan RSG Sembiring ◽  
Gusti Meliyanie

Abstract. Aedes albopictus can act as a primary or secondary vector of dengue hemorrhagic fever andchikungunya. Its population needs to be controlled. One of the environmentally-friendly control methods isthe use of ovitrap. Ovitrap will be more optimal if it added by attractant substances from easily obtainedingredients in the surrounding environment. This study aims to determine the effectiveness of attractants inthe form of water-soaked paddy (Oryza sativa), cogongrass (Imperata cylindrica) and Indian goosegrass(Eleusine indica). An experimental study with a completely randomized design (CRD) was carried out at theEntomology Laboratory of Balai Litbangkes Tanah Bumbu in January - April 2018. The population of thisstudy is the 10th filial of Ae. albopictus laboratory colonization and gravid female mosquito as samples.Material combination in the study was paddy straw soaking water (ARJP), Indian goosegrass immersionwater, cogongrass soaking water, Ae albopictus used eggs laying water (ABT), and distilled water as control(K). Repetition was done five times. Effectiveness of attractants analyzed by ANOVA and LSD tests. Datanormality tested by Kolmogorov-Smirnov test, if it was not fulfilled, the Kruskal Wallis test was used. Theresults showed that the use of attractants of water-soaked paddy, Indian goosegrass and cogongrass provedto have a different effect on the number of Aedes albopictus mosquito eggs compared to aquades and eggslaying water, but there was no influence between the three types of attractants.


2019 ◽  
Vol 7 (1) ◽  
pp. 1
Author(s):  
Danie Indra Yama ◽  
Jojon Soesatrijo ◽  
Rusnadi Santiko

The aim of this research is to knowtime of death, mortality and changes in behavior of Oxya chinensis in the treatment of botanical insecticides root of the tuba. The research was conducted at Laboratory Citra Widya Edukasi Palm Oil Polytechnic in Bekasi, the experimental design was the Completely Randomized Design, the treatment consisted of A0 = deltamethrin 0.3 cc/100 ml, A1 = tuba root extract 3g/100ml, A2 = tuba root extract 4g/100ml , A3 = tuba root extract 5g/100ml. Each treatment was repeated three times and each replication contained of 3 samples. Data were analyzed using variance with 5% level, if there is a real effect then tested further with the test of the Least Significance Different. Making extracts by means of tuba roots chopped until smooth, mixed with 100 ml of water, saved for 3 days, then filtered and taken water and the application is sprayed. Pest control using chemical insecticides is more effective than botanical insecticides root of the tubabut 4g/100ml botanical insecticides toba root are also potentially in Oxya chinensis death at 39,51 minutes after the application. The application of chemical and botanical insecticides made from tubaroots causes Oxya chinensis to decrease activity than dies.


ZOOTEC ◽  
2016 ◽  
Vol 37 (1) ◽  
pp. 15
Author(s):  
Novalia Lumban Gaol ◽  
Ch. L. Kaunang ◽  
Rustandi . ◽  
F. Dompas

INFLUENCE OF CONCENTRATION AND SOAKING TIME OF A. pintoi IN THE COW URINE ON PLANT GROWTH. Cow urine has an auksin a, auksin b, and IAA (hetero auksin) that can stimulate plant roots. The present study was conducted to determine the influence of concentration and soaking time in the cow urine on Arachis pintoi (A. pinto) growth. This study used Completely Randomized Design (CRD) in Factorial arrangement with 2 factors A and B. Factor A= urine concentration: A1 = urine 75% + water 25% and A2; urine 50% + water 50%; and factor B = soaking time: B1 = 1 hour; B2 = 2 hours; Dan, B3 = 3 hours. Treatments were replicated 4 times. Parameters measured were: leaves amount (sheets), plant length (cm), and root weight (g). Research results showed that mean leaves amount ranged from 41,65 to 46,77 sheets, plant length 35,97-36,67 cm, and root weight 1,95-1,72 g. Utilization of cow urine with a different concentration and soaking time gave a non significant different (P>0.05) on leaves amount, plant length, and root weight. It can be concluded that cow urine concentration and soaking time do not effect leaves amount, plant length, and root weight of Arachis pintoi (A. pinto). Key words:  Cow urine concentration, Soaking time,  A. pintoi.


2018 ◽  
Vol 5 (1) ◽  
pp. 1-12
Author(s):  
Asrul . Ferdian ◽  
Muslim . ◽  
Mirna Fitrani

ABSTRACT The use of extract root ginseng in the process of Betta fish masculinization is expected to increase the testosterone. The purpose of this research is to determine the effect ginseng root extract to the percentage of the male betta fish by masculinization. This study has been conducted at the Basic Aquaculture Laboratory, Faculty of Agriculture, Sriwijaya University. This study use a completely randomized design with 4 treatments and 3 replications. The treatments in this research was betta fish larvae aged 7 days that submersed on ginseng root extract media with different concentration for 8 hours. The treatment were the addition of ginseng root extract as much as 1 mg/L (P1), 2 mg/L (P2), 3 mg/L (P3), 4 mg/L (P4) and without ginseng root extract addition as control (P0). Parameters observed consist of percentage of betta fish male, survival rate and quality of water. The result of this research shows that the addition of extract panax ginseng with difference concentration has significant effect to male betta fish percentage, but there is no a significant effect on survival rate. Treatment P3 with concentration 3 mg/L is the best result, it produced 95.05% of male betta fish after immersion for 8 hours. Keywords : Betta fish, Ginseng root extract, Immersion, Masculinization


Author(s):  
Alireza Yadavi ◽  
Parvin Pireh ◽  
Hamidreza Balouchi

A greenhouse experiment was conducted to study the combined effects of cadmium toxicity, application of vermicompost and inoculation of arbuscular mycorrhiza on soybean. The experiment was laid out in a completely randomized design (factorial) and replicated thrice. The treatments included arbuscular mycorrhiza in two levels (inoculation and non- inoculation in soil), vermicompost in two levels (application @ 5 % of soil weight and non-application) and cadmium chloride in five levels (0, 20, 40, 80 and 160 mg kg-1 of soil). The result showed that increasing concentrations of cadmium chloride significantly increased electrolyte leakage and leaf transpiration, and decreased photosynthetic rate, Fv/Fm, root weight, pod number per plant and grain weight per plant. Application of vermicompost and mycorrhiza decreased the toxic effects of cadmium chloride. Application of vermicompost increased Fv/Fm, grain weight per plant, number pods per plant and seed oil percent, and application of mycorrhiza increased root weight and photosynthetic rate.


BioScience ◽  
2019 ◽  
Vol 3 (1) ◽  
pp. 69
Author(s):  
Yenni Fitri ◽  
Elsa Yuniarti

Traditional medicine is one of the drugs used by the community to be one of the efforts to treat diseases. One of them is Diabetes Mellitus (DM). DM is a metabolic disease characterized by hyperglycemia which results in an increase in free radicals in the cell. DM treatment is quite expensive so an alternative drug is needed. One of them is Tithonia diversifolia A. Gray. This study used a completely randomized design, consisting of 1 control, 4 treatments (P1: Only alloxan induced), P2: Alloxan 65 mg / kg BB, P3: Metformin 65 mg / kg BB, P4: Boiled leaves of moon leaves 24.6 mg / 10 ml and P4: Moon flower leaves boiled water 49.1 mg / 10 ml. The parameters observed were blood sugar in male mice and the number of cells in the islands of Langerhans before and after being given boiled leaves of moon flowers and metformin. Data were analyzed using ANOVA then continued with DMRT test with a significant difference of 5%. The results showed that administration of boiled kembang bulan leaves and metformin for 7 days could reduce blood sugar levels in mice and accelerate the regeneration of pancreatic cells. The most significant impact is the treatment with the highest dose, namely P4 (Moon leaf leaves boiled water 49.1 mg / 10 ml).


2021 ◽  
Vol 52 (2) ◽  
pp. 29-38
Author(s):  
A.M. Oyeleke ◽  
O.A. Adeyemi ◽  
L.T. Egbeyale ◽  
R.A. Sobayo ◽  
R.O. Olaifa

Abstract This study investigated the response of laying hens to aqueous extracts of Petiveria alliacea root and leaf. A total of 288 eighteen-week-old Isa brown pullets were used for the 25-week study. The pullets were arranged in a 2 × 4 factorial experimental layout in a completely randomized design. The pullets were distributed into two groups administered root extract or leaf extract. Pullets in each group were allotted to four subgroups administered aqueous extracts of Petiveria alliacea at 15, 30 and 45 g l–1 concentration levels making eight treatments in total. Each treatment was replicated three times with twelve pullets per replicate. Eimeria oocyst counts and intestinal bacteria counts were lower (P < 0.0001 and P = 0.0028, respectively) in hens administered 15, 30 and 45 g l–1 of Petiveria alliacea extracts than the control. The highest (P < 0.0001) antibody titre against Newcastle disease vaccine was recorded in hens administered 30 and 45 g l–1 concentrations of root (9.06 and 9.10 log2, respectively) and leaf (9.08 and 9.18 log2, respectively) extracts. The liver sections of hens in all treatments appeared normal. In conclusion, aqueous extract of Petiveria alliacea root and leaf at 30 and 45 g l–1 concentrations performed best as antimicrobial and immune stimulating agent without impairing liver health.


2019 ◽  
Vol 2 (1) ◽  
pp. 399-412
Author(s):  
Tri Bagus Wicaksono ◽  
Saifuddin Hasjim ◽  
Nanang Tri Haryadi

Golden snail (Pomacea canaliculata L.) is one of the main pests that attack young paddy plants. Golden snail pest attack on paddy plants has the potential to reduce yields and even crop failure. Kipahit plants (T. diversifolia) have the potential as bio molluscides because they contain toxic compounds against golden snails. The purpose of this research was to study the effect of bio molluscicide concentration of kipahit leaf extract (T.diversifolia) on mortality of golden snails at two phases of age and damage intensity of paddy  plants. The. The research was conducted in December 2017 until May 2018. The research was carried out in Lampeji Village, Mumbulsari District, Jember Regency. The researh used a Completely Randomized Design (CRD) with 6 concentrations of kipahit leaf extract, tested on 2 different age phases of golden snail and 3 times repeated. The variables observed were golden snail mortality, crop damage intensity (DI), Lethal Concentration 50 (LC50) and Lethal Time 50 (LT50). The data obtained were then analyzed by probit analysis and Duncan test with a confidence level of 95%. The results showed that the concentration of 60 g/L was the most efficient and effective concentration in controlling golden snails at various age phases as well as suppressing the intensity of damage to paddy plants. Keywords: golden snail, kipahit leaves (T. diversifolia), molluscicide, mortality


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