scholarly journals Uji Antibakteri Obat Kumur Ekstrak Etanol Tanaman Ciplukan (Physalis angulata l.) Terhadap Bakteri Gram Positif

2020 ◽  
Vol 13 (1) ◽  
pp. 103
Author(s):  
Teti Rostikawati
Keyword(s):  
Antimicrobial Activity ◽  
Streptococcus Mutans ◽  
Research Method ◽  
The Body ◽  
Ethanol Extraction ◽  
Power Test ◽  
Experimental Laboratory ◽  
Kill Rate ◽  
Physalis Angulata ◽  
Streptococcus Mutants

Abstrak: Mulut memiliki mikrofloranormal bakteri sebagai pertahanan tubuh. Bakteri Streptococcus mutans merupakan bakteri yang berkelompok pada permukaan gigi yang memiliki peranan dalam pembentukan karies gigi. Daun dan batang Ciplukan (Physalis angulata L.) mengandung polifenol, flavonoid, dan etanol serta antioksidan yang kuat yang diduga memiliki aktivitas antimikroba yang sangat baik. Tujuan penelitian ini yatu untuk mengetahui daya antibakteri obat kumur ekstraksisi etanol campuran daun dan batang ciplukan (P. angulata L.) terhadap bakteri Streptococcus mutans. Metode penelitian bersifat eksperimental murni laboratorium yang menggunakan kulturisasi bakteri S. mutans.�Uji daya antibakteri menggunakan cara dengan dilusi cair dan dilanjutkan dengan dilusi padat. Uji analisis statistik menggunakan uji deskriptif. Dari hasil penelitian menunjukkan bahwa pada konsentrasi 15% obat kumur ekstraksi etanol campuran daun dan batang ciplukan mempunyai kadar hambat minimal (KHM) dan kadar bunuh minimal (KBM).� �Formulasi ini bisa direkomendasikan untuk dilakukan penelitian lebih lanjut agar bisa digunakan oleh masyarakat yang tinggal dikawasan suhu dingin.Kata Kunci: Ciplukan, obat kumur, Streptoccus mutans.�Abstrak: The mouth has microfloranormal bacteria as a defense of the body. Mutant Streptococcus bacteria are bacteria that cluster on the surface of the teeth that have a role in the formation of dental caries. Ciplukan leaves and stems (Physalis angulata L.) contain polyphenols, flavonoids, and ethanol as well as powerful antioxidants that are thought to have excellent antimicrobial activity. The purpose of this study was to find out the antibacterial power of ethanol extraction drugs mixed with leaves and stems ciplukan (P. angulata L.) against bacteria Streptococcus mutants. The research method is purely experimental laboratory that uses the culture of bacteria S. mutants.�Antibacterial power test using a means with liquid dilution and continued with solid dilution. Test statistical analysis using descriptive tests. From the results of the study showed that at a concentration of 15% mouthwash ethanol extraction mixed leaves and stems ciplukan have minimal levels of bland (KHM) and minimal kill rate (KBM).� �This formulation can be recommended for further research so that it can be used by people living in cold temperatures.Keywords: �Ciplukan, mouthwash, Streptoccus mutants�

The role of intracanal medicaments in inhibition of bacteria isolated from root canals of infected primary molars

2019 ◽  
Vol 14 (1) ◽  
pp. 92
Author(s):  
Dr. Maha Abdul- Kareem Mahmood ◽  
Dr. Huda Elias Ali ◽  
Dr. Haraa Khairi Abdul-Kadher
Keyword(s):  
Antimicrobial Activity ◽  
Streptococcus Mutans ◽  
Normal Saline ◽  
Root Canal ◽  
Primary Teeth ◽  
Primary Molars ◽  
Root Canals ◽  
Etiologic Agents ◽  
Micro Organisms

Microbes are considered as the primary etiologic agents in endodontic diseases.Disinfection of the root canal is obtained by the combined effect of biomechanicalpreparation, irrigation and intra canal medicament. The aim of the present study wasto assess the antimicrobial activity of intracanal medicaments (formocresol andEndosepton) against two micro organisms (Streptococcus mutans and staphylococcusaureus) isolated from 15 necrotic pulps of primary molars indicated for pulpectomyprocedure. The samples were cultured, and purified using microbiological evaluation.Broth dilution test was performed in our study by preparing test tubes containing10 ml of BHI broth (pH. 7) which then inoculated with strains of the tested bacteriaand incubated at 37 C° for 24 h. After over night incubaction, ten fold dilution weremade in test tubes containing 9 ml of normal saline by adding 1 ml of the inoculum tothe first tube . Then from dilution 10-1 , 0.1 ml of cell suspension was added to 9.9 mlof formocresol and endosepton, then 0.1 ml was taken and spread on duplicates ofBHI agar plates at different intervals and incubated aerobically for 24 h. at 37 C°.Colonies on the plates were counted after incubation and CFU/mL (colony formingunit) was calculated. Our results indicating that there were no significant differencesbetween the intracanal medicaments, but there were high significant differencesbetween the intervals time of the study. We concluded that both materials had greatantibacterial effect against the pathogens commonly isolated from necrotic pulpaltissue of primary teeth.

scholarly journals Viable and Heat-Killed Probiotic Strains Improve Oral Immunity by Elevating the IgA Concentration in the Oral Mucosa

2021 ◽  
Author(s):  
Wen-Yang Lin ◽  
Yi-Wei Kuo ◽  
Ching-Wei Chen ◽  
Yu-Fen Huang ◽  
Chen-Hung Hsu ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
The Body ◽  
Effective Dosage ◽  
Tgf Beta ◽  
Oral Tablet ◽  
Beneficial Effects ◽  
Bifidobacterium Animalis ◽  
Probiotic Strains ◽  
Oral Pathogens

AbstractOral-nasal mucosal immunity plays a crucial role in protecting the body against bacterial and viral invasion. Safe probiotic products have been used to enhance human immunity and oral health. In this study, we verified the beneficial effects of mixed viable probiotic tablets, consisting of Lactobacillus salivarius subsp. salicinius AP-32, Bifidobacterium animalis subsp. lactis CP-9, and Lactobacillus paracasei ET-66, and heat-killed probiotic tablets, consisting of L. salivarius subsp. salicinius AP-32 and L. paracasei ET-66, on oral immunity among 45 healthy participants. Participants were randomly divided into viable probiotic, heat-killed probiotic, and placebo groups. The administration of treatment lasted for 4 weeks. Saliva samples were collected at Weeks 0, 2, 4, and 6, and Lactobacillus, Bifidobacterium and Streptococcus mutans populations and IgA concentration were measured. IgA concentrations, levels of TGF-beta and IL-10 in PBMCs cells were quantified by ELISA method. Results showed that salivary IgA levels were significantly increased on administration of both the viable (119.30 ± 12.63%, ***P < 0.001) and heat-killed (116.78 ± 12.28%, ***P < 0.001) probiotics for 4 weeks. Among three probiotic strains, AP-32 would effectively increase the levels of TGF-beta and IL-10 in PBMCs. The oral pathogen Streptococcus mutans was significantly reduced on viable probiotic tablet administration (49.60 ± 31.01%, ***P < 0.001). The in vitro antibacterial test confirmed that viable probiotics effectively limited the survival rate of oral pathogens. Thus, this clinical pilot study demonstrated that oral probiotic tablets both in viable form or heat-killed form could exert beneficial effects on oral immunity via IL-10, TGB-beta mediated IgA secretion. The effective dosage of viable probiotic content in the oral tablet was 109 CFUs/g and the heat-killed oral tablet was 1 × 1010 cells/g.

Evaluation of Antibacterial and Antifungal Properties of ZnO and Silver Nanoparticles against Candida Albicans and Streptococcus Mutans in Acrylic Resin

2021 ◽  
Author(s):  
Amin Khaleghi ◽  
Amin Archin ◽  
Mojtaba Azarian Borujeni
Keyword(s):  
Candida Albicans ◽  
Streptococcus Mutans ◽  
Zno Nanoparticles ◽  
Acrylic Resin ◽  
Spherical Particles ◽  
Significance Level ◽  
Ultrasonic Apparatus ◽  
Experimental Laboratory ◽  
Antibacterial And Antifungal ◽  
Post Hoc

Introduction: This study aimed to evaluate the antibacterial and antifungal effect of Ag and zinc oxide nanoparticles to complete denture acrylic resin. Materials & Methods: In this experimental laboratory study, 144 samples were evaluated. The powder of acrylic resin (PMMA Ivoclar Vivadent, Lichenstein SR Triplex Hot) was mixed homogeneously in the ultrasonic apparatus with Ag (purity: 99.99%, size: 20nm, spherical) and ZnO (purity: 99%, size: 10-30 nm, nearly spherical) particles with mass fraction 0.5, 1 ,2 and 5. The microorganisms of this study were Candida albicans and Streptococcus mutans. The growth rate of microorganisms was measured by spectrophotometer based on turbidity and readings at 600 nm. Data were analyzed by ANOVA and Tukey post hoc tests. The significance level was set at 0.05. Results: Increasing Ag and ZnO nanoparticles to acrylic resin reduced the growth of Candida Albicans and Streptococcus Mutans. Even the concentration of 0.5% significantly reduced the growth of microorganisms. Complete growth inhibition was in the concentration of 5% after 24 and 48 hours. Conclusion: Increasing the Ag and ZnO nanoparticles to acrylic resin reduced the growth of microorganisms.

scholarly journals FORMULASI DAN AKTIVITAS ANTIBAKTERI SEDIAAN OBAT KUMUR DARI EKSTRAK ETANOL 96% DAUN CIPLUKAN (Physalis angulata L.) TERHADAP BAKTERI Streptococcus mutans

2020 ◽  
Vol 5 (2) ◽  
pp. 70-82
Author(s):  
Nadhiffa Anisa
Keyword(s):  
Streptococcus Mutans ◽  
Chlorhexidine Gluconate ◽  
Plate Technique ◽  
Physalis Angulata

Telah dilakukan penelitian tentang aktivitas antibakteri sediaan obat kumur dari ekstrak etanol 96% daun ciplukan (Physalis angulata L.) terhadap bakteri Streptococcus mutans dengan metode cup plate technique dan disertai uji stabilitas fisik sediaan obat kumur. Secara empiris, daun ciplukan digunakan untuk penyembuhan luka, radang hati, malaria, penyakit kelamin, rematik, sakit telinga, dan menghambat pertumbuhan kanker terutama kanker usus besar. Ekstrak daun ciplukan  diketahui mengandung polifenol, alkaloid, dan flavonoid. Penelitian ini bertujuan untuk mengetahui besarnya konsentrasi ekstrak etanol 96% daun ciplukan yang sudah memiliki aktivitas antibakteri serta mencari konsentrasi terbaik ekstrak etanol 96% daun ciplukan dalam bentuk sediaan obat kumur untuk uji aktivitas antibakteri terhadap bakteri Streptococcus mutans. Penelitian ini menggunakan 7 kelompok perlakuan dengan konsentrasi ekstrak 10%, 20%, 30%, 40%, 50%, kontrol positif (chlorhexidine gluconate 0,2%), serta kontrol negatif (aquadest steril). Uji aktivitas antibakteri ekstrak daun ciplukan terhadap bakteri Streptococcus mutans dilakukan sebagai uji pendahuluan dan hasil yang didapatkan Konsenterasi Hambat Minimum (KHM) terhadap  pertumbuhan  bakteri  Streptococcus  mutans  ada  pada  konsenterasi ekstrak 10% dengan rata-rata zona hambatan sebesar 7,57 mm. Sedangkan pada uji aktivitas antibakteri obat kumur daun ciplukan didapatkan hasil Konsentrasi Hambat Minimum (KHM) pada konsentrasi obat kumur 10% dengan rata-rata zona hambat sebesar  8,53 mm. Formulasi obat kumur yang terbaik ada pada  konsentrasi 50% dengan rata-rata  zona hambat berintensitas kuat yaitu sebesar 10,15 mm. Evaluasi stabilitas dari sediaan obat kumur meliputi pemeriksaan organoleptis, homogenitas dan pH yang dilakukan  selama tujuh minggu  penyimpanan  pada suhu  ruang  menunjukkan hasil yang stabil.Kata kunci : Ekstrak daun ciplukan (Physalis angulata L.); Antibakteri;  Streptococcus mutans; difusi cup plate technique. 

scholarly journals THE REDESIGN STRATEGY AT REGION GENERAL HOSPITAL TYPE B IN MAGELANG CITY

Arsitektura ◽  
2018 ◽  
Vol 16 (1) ◽  
pp. 15
Author(s):  
Prananda Fadhlul Husna ◽  
Sri Yuliani ◽  
Ahmad Farkhan
Keyword(s):  
Health Care ◽  
General Hospital ◽  
Research Method ◽  
The Body ◽  
Type B ◽  
Hospital Type ◽  
Analysis Study ◽  
Planning And Design ◽  
Hospital Building ◽  
Medical Equipments

<p class="Abstract"><em></em><em><em>Health is the prosperous state of the body, soul and social that must always be maintained by way of periodic checks to the hospital. The hospital is health care institutions that provides patient care with medical personsl, specialized nurses and medical equipments that need to be accredited to improve public services. The purpose of this redesain strategy is to formulate the concept of planning and design of Tidar Hospital of Magelang City that meet the criteria of the future. Redesign of RSUD Tidar in Magelang was conducted based on Hospital Building Technical Guidelines in 2012. </em><em>The research method is divided into three levels, programming, planning and design. Based on the results of the analysis study in field, the redesign has been obtained from the application of the Hospital Building Technical Guidelines. It should be designed on the outpatient installation can make the waiting area wider and the circulation of patients with the officers can be separated. The parking area is placed on the basement area so it is wider. For Emergency Installation is placed close to the main door so that the patient can go directly to this area and the circulation separate from the syringe to the inpatient and service area. Most of room programmes are designed based on universal accessibity.</em></em></p><p class="Abstract"><em>                                               </em></p>

scholarly journals Collagen drinks for functional nutrition

2018 ◽  
Vol 80 (3) ◽  
pp. 97-103
Author(s):  
L. V. Antipova ◽  
S. A. Storublevtsev ◽  
A. A. Getmanova
Keyword(s):  
Connective Tissue ◽  
Main Part ◽  
Jerusalem Artichoke ◽  
The Body ◽  
Whole Body ◽  
Energy Functions ◽  
Preventive Actions ◽  
Prevention And Treatment ◽  
Experimental Laboratory

In the process of life of the body continuously consumed nutrients that perform plastic and energy functions. The source of nutrients is a variety of foods, consisting of a complex of proteins, fats and carbohydrates, which in the process of digestion are converted into digestible substances. Collagen is the basis of connective tissue and binds the cells in the tissues, creates the frame of the whole body. The gastrointestinal tract, as a system of organs, is no exception and is designed process and extract nutrients from food. Most organs consist of connective tissue, accounting for 60–90% of their mass, which confirms its importance and the role of collagen in this regard can not be estimated. Collagen functions in the body are diverse, one of the main - part in digestion, the violation of which is the cause of diseases such as gastritis and ulcers. For the prevention and treatment of such diseases are very useful liquid collagen-containing food in the form of functional drinks. Developed and obtained in the experimental laboratory a variety of drinks on a collagen basis, with the use of additional broth with sea buckthorn pulp, tincture of dried chicory root powder and broth with the flesh of Jerusalem artichoke. An invaluable contribution to the therapeutic and preventive actions of all these components is proved not only scientifically, but also time-tested.

scholarly journals Antimicrobial activity of Cymbopogon citratus (Poaceae) essential oil on Streptococcus mutans biofilm and cytotoxic effect on keratinocytes and fibroblasts

2018 ◽  
Vol 66 (4) ◽  
pp. 1519
Author(s):  
Mailen Ortega Cuadros ◽  
Adriana Patricia Tofiño Rivera ◽  
Luciano Jose Merini ◽  
Maria Cecilia Martinez Pabon
Keyword(s):  
Antimicrobial Activity ◽  
Dental Caries ◽  
Essential Oil ◽  
Streptococcus Mutans ◽  
Human Fibroblasts ◽  
Positive Control ◽  
Negative Control ◽  
Cymbopogon Citratus ◽  
Significant Difference ◽  
Efficient Alternative

Dental caries is a pathology of multifactorial origin and currently natural products are an efficient alternative treatment; The work sought to evaluate the antimicrobial activity of the Cymbopogon citratus essential oil and the citral and myrcene components against Streptococcus mutans ATCC UA159, as well as their cytotoxicity on keratinocytes and human fibroblasts. The viability effect against Streptococcus mutans on biofilms was evaluated through exposure to the three substances by using the MBEC technique-high-throughput at concentrations of 1, 0.1, and 0.01 µg/mL and chlorhexidine as positive control. The cytotoxicity of the compounds was evaluated on keratinocytes and fibroblasts through the MTT reduction technique, using 0.5 mM H2O2 as cell-death control (negative control) and ethanol 1% as vehicle control (positive control). The three substances evaluated had effects on the viability of Streptococcus mutans with mortality between 74% and 96%, without significant difference among them (p > 0.393); additionally, no cytotoxicity was evident on keratinocytes and fibroblasts in a 24-h treatment. The substances evaluated showed significant antimicrobial effects; hence, these should be studied further as potential co-adjuvants to prevent dental caries that cause minor adverse effects

scholarly journals The influence of a relaxing massage on stress levels in women

2021 ◽  
Vol 10 (1) ◽  
pp. 19-22
Author(s):  
Marta Majewska-Pulsakowska ◽  
◽  
Martyna Mączka ◽  
Keyword(s):  
Perceived Stress ◽  
Research Group ◽  
Research Method ◽  
Negative Symptoms ◽  
Subjective Assessment ◽  
The Body ◽  
Control Group ◽  
Stress Scale ◽  
Stress Levels ◽  
Lower Education

One of the factors that negatively affect the human body is susceptibility to stress. The inability to react to negative emotions and reduce tensions in the body causes many negative symptoms that affect people every day. A relaxing massage is one of the ways to reduce stress levels. The aim of the study was to attempt to assess the effect of a relaxing massage on the level of stress in women aged 25-45 years. The research group consisted of 60 women. The research group underwent a proprietary relaxing massage, while the control group used manicures or pedicures. As the research method an original questionnaire and the PSS-10 (Perceived Stress Scale) was used. The subjective assessment of the level of stress based on the proprietary survey and the PSS-10 questionnaire among the respondents after the relaxing massage was statistically signifi cantly lower. How ever, no statistically signifi cant differences in the decrease in stress levels were found between the two groups. On the other hand, a statistically signifi cant relationship between education and the level of stress was proved. Subjects with lower education were characterized by a higher level of stress.

scholarly journals MERINTANGI PENGUBURAN JENAZAH COVID-19 DITINJAU BERDASARKAN PASAL 178 KITAB UNDANG-UNDANG HUKUM PIDANA

LEGALITAS ◽  
2021 ◽  
Vol 5 (2) ◽  
pp. 104
Author(s):  
Muhammad Rezky Rinaldy Dan Syamsudin
Keyword(s):  
Law Enforcement ◽  
Research Method ◽  
Criminal Code ◽  
The Body ◽  
Legal Research ◽  
Corona Virus ◽  
The World ◽  
Law Enforcement Officials ◽  
The Impact

Indonesia and even the world now feel the impact of the Corona virus outbreak (covid-19), in connection with it hindering the burial of the bodies of victims who died. The phenomenon of corpse rejection of corona virus patients (covid-19) continues to occur in various regions. In fact, the body must be buried immediately no later than 4 hours after being declared dead. The main reason people are reluctant to accept the bodies of patients co-19 because of fear of contracting. While the medical ensure that the body will not transmit the virus. The body in the coffin has been wrapped and declared sterile. The type of research used in this study is the type of normative legal research, which is a legal research method that uses a statutory approachThe results of the study showed that obstructing officers who will carry out official burials could indeed be convicted. Law enforcement officials can use Article 178 of the Criminal Code. not a complaint offense. Law enforcement officials can immediately take action without anyone complaining. "If the incident fulfills the elements contained in Article 178 of the Criminal Code, the perpetrators can be charged. However, it must look at intentions and actions as a condition for imposing a crime on someone.

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