CHEMICAL COMPOSITION OF EXTRACTS OF GINSENG REAL ROOT (PANAX GINSENG) OBTAINED BY SEQUENTIAL EXTRACTION WITH DIFFERENT-POLAR SOLVENTS

Author(s):  
Т.В. ПЕЛИПЕНКО ◽  
Н.М. АГЕЕВА ◽  
В.И. АБАКУМОВ ◽  
А.С. ТЕСЛЕНКО ◽  
В.А. РОСТОВЦЕВА

Экстракцию корней женьшеня осуществляют водно-спиртовыми растворами или водой с последующим консервированием этиловым спиртом. При этом отмечается неполное извлечение биологически активных компонентов. Анализ химического состава корней женьшеня показал, что в них содержатся вещества различной полярности, поэтому целесообразно для лучшего извлечения экстрактивных веществ (ЭВ) проводить экстракцию этого лекарственного сырья в три этапа с использованием в качестве растворителей сжиженного диоксида углерода, этилового спирта и воды. Объектами исследования были экстракты, полученные из двух образцов измельченных корней женьшеня: из образца сырья 1 получены при последовательной экстракции СО2-экстракт из исходного сырья, спиртовой и водный экстракты; из образца сырья 2 – спиртовой экстракт из исходного сырья и водный экстракт из сырья, подвергнутого экстракции этиловым спиртом. Установлено, что последовательная экстракция сжиженным диоксидом углерода, этиловым спиртом и водой увеличивает выход ЭВ на 7% в сравнении с экстракцией только этиловым спиртом и водой. В составе СО2-экстракта корней женьшеня содержание токоферолов и других соединений фенольного ряда составляет 3,8%, каротиноидов 0,04%. Предварительная экстракция сжиженным диоксидом углерода корней женьшеня увеличивает выход органических кислот, флавоноидов и анионов солей в 5,8 раза; органических веществ, определенных методом ГЖХ, в 2,5 раза; моно-, ди- и полисахаридов в водном экстракте на 33,2% . В спиртовых экстрактах отмечено наибольшее содержание минеральных веществ: макроэлементов – магния, калия и фосфора; микроэлементов – марганца, железа, меди и йода. Общий выход сапонинов при последовательной экстракции корней женьшеня, включающей СО2-экстракцию, увеличивается на 9,7%. Результаты исследования подтверждают целесообразность разработки технологии комплексной переработки корней женьшеня, включающей применение в качестве растворителей сжиженного диоксида углерода, этилового спирта и воды. Extraction of ginseng roots is carried out with water-alcohol solutions or water, followed by preservation with ethyl alcohol. At the same time incomplete extraction of biologically active components is noted. Analysis of the chemical composition of ginseng roots shows that they contain substances of different polarities, so it is advisable to extract this medicinal raw material in three stages using liquefied carbon dioxide, ethyl alcohol and water as solvents for better extraction of extractive substances (ES). The objects of the study were extracts obtained from two samples of crushed ginseng roots: from a sample of raw materials 1, CO2-extract from the feedstock, alcohol and water extracts were obtained by sequential extraction; from a sample of raw materials 2 an alcohol extract from the feedstock and water aqueous extract from raw materials extracted with ethyl alcohol. It was found that sequential extraction with liquefied carbon dioxide, ethyl alcohol and water increases the ES yield by 7% in comparison with extraction with only ethyl alcohol and water. In the composition of the CO2-extract of ginseng roots, the content of tocopherols and other phenolic compounds is 3,8%, carotenoids 0,04%. Preliminary extraction of ginseng roots with liquefied carbon dioxide increases the yield of organic acids, flavonoids and anions of salts by 5,8 times; organic substances determined by the gas-liquid chromatography method by 2,5 times; mono-, di- and polysaccharides in an aqueous extract by 33,2%. The alcohol extracts have the highest content of minerals: macronutrients – magnesium, potassium and phosphorus; microelements – manganese, iron, copper and iodine. The total yield of saponins during sequential extraction of ginseng roots, including CO2-extraction, increases by 9,7%. The results of the study confirm the feasibility of developing a technology for complex processing of ginseng roots, including the use of liquefied carbon dioxide, ethyl alcohol and water as solvents.

2020 ◽  
Author(s):  
Oleg Spichak

The monograph covers issues related to the use of bee products and their standardized substances in medicine, pharmacy and cosmetology. The publication describes the products of beekeeping, in particular (honey, propolis, flower pollen (bee pollen), bee venom, perga) as natural raw materials for production based on them in industrial conditions and in the conditions of pharmaceutical manufacture of pharmaceuticals and cosmetics. in order to provide the population with effective domestic natural medicines as an alternative method of treatment of various diseases. Characteristics, chemical composition and features of use of biologically active standardized substances on the basis of beekeeping products developed in Ukraine are presented: propolis phenolic hydrophobic drug, aqueous extract of propolis, honey, powdered honey, liphophilic extract of bee stalk, fermentation, substances of bee venom, perga, big bee larva biomass. The technology of production of extemporaneous medicinal apipreparations of different direction of action in the conditions of pharmacies is given. The publication is intended for a wide range of specialists of pharmacies in Ukraine, scientists and practitioners of pharmacy, medicine, apitherapy, beekeeping and cosmetology.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Rong Yang ◽  
Yu Zhang ◽  
Sailesh Ranjitkar ◽  
Mingxiang Li ◽  
Yongjie Guo ◽  
...  

AbstractHumans have dyed textiles and leather, colored food, and paint body parts using natural dyes throughout history. Natural dyes have suffered drastically due to recent socioeconomic changes and replacement with synthetic dye in the textile industry. Hence, it is urgent to study indigenous dye plants and dyeing craft in local communities to protect these resources' potential ecological, economic, and cultural values. In 11 Monpa villages of Mêdog County, South-east Tibet, China, we conducted field research to record the indigenous method of cloth dyeing using madder dye. An aqueous extract of the root of Rubia wallichiana is a traditional madder dye. In this study, we used traditional dye and ethyl alcohol extract of the residue of aqueous extract. Two fabrics were dyed with the extractions in the presence of one of the metallic mordants or biomordants. Pigment compounds from aqueous extraction of madder and ethyl alcohol extraction of recycled madder were evaluated using Phytochemical, UV–visible spectroscopy, and FTIR test. We carried out One-way ANOVA and Duncan's new multiple range method to analyze different dying approaches and conditions. The dyed fabrics were evaluated by indicators of color strength and fastness, including washing, rubbing, and perspiration. The findings revealed the potentiality of biomordants to improve the dyeing properties of madder. The dyeing properties of recycled madder were marginally better than traditional madder. The results revealed the feasibility of enhancing the dyeing property and reuse of the residue from madder dyeing. The improved dyeing and reuse of residue can improve local ecological, economic benefits, and cultural heritage while applying research findings for the subsequent commercialization of plant dyes.


2021 ◽  
Vol 2 (446) ◽  
pp. 6-13
Author(s):  
G.E. Azimbayeva ◽  
A.K. Kamysbayeva ◽  
Abdigali Bakibaev

In recent years, serious attention has been paid around the world to the chemical and biotechnological processing of biomass of easily renewable plant raw materials. One of the most mass-produced aliphatic alcohols is ethyl alcohol. The aim of this work was gas chromatographic analysis of the chemical composition of ethyl alcohol obtained from plants of the genus Asteraceae (dahlia and jerusalem artichoke tubers, chicory and big burdock roots) growing on the territory of Kazakhstan. The article discusses the methods of obtaining and application of ethyl alcohol. The results of the analysis of the chemical composition of ethyl alcohol obtained from plants of the genus Asteraceae are presented. As a result of the study, alcohols were obtained in different concentrations. The chemical composition of ethyl alcohol was determined by gas chromatography. The impurity composition of ethyl alcohol obtained from various types of raw materials is also shown. The composition of impurities in various samples of ethanol and alcohol-containing products is considered in detail. The results of the study were discussed, and the peculiarities of using the gas chromatography method were shown.


2021 ◽  
Vol 3 (3(59)) ◽  
pp. 46-49
Author(s):  
Kateryna Kasabova ◽  
Nataliа Shmatchenko ◽  
Aleksey Zagorulko ◽  
Andreii Zahorulko

The object of research in this work is the technology of pastille with the addition of multicomponent fruit and berry paste. Pastille are becoming more and more popular in the world for all segments of the population. Usually, the main ingredients for their manufacture are fruit raw materials, a structurant and egg white, therefore, the products contain practically no fat and have a low calorie content. It is proposed to replace 75 % of traditional applesauce in the recipe with a multicomponent fruit and berry paste made from apples, cranberries, hawthorn. It was found that such a composition makes it possible to obtain products with an increased content of pectin substances, ascorbic acid, polyphenols, including anthocyanins, catechins and flavonols. The work is aimed at studying the application of the principles of qualimetry to determine a complex indicator of the quality of pastille For this, the organoleptic and physicochemical indicators of the quality of the developed pastille were taken from previous studies and the chemical composition was determined and the content of biologically active substances was calculated relative to the average daily human need. The content of pectin substances in new products increases 1.7 times and satisfies the average daily human need by 18.2 %. The content of ascorbic acid is 4.2 times higher, which is 20.5 % of the average daily human need, and polyphenols 3.2 times higher – 579.0 %. The data obtained make it possible to consider the developed product functional. The next step was to determine the complex indicator of products by the qualimetric method. For a more complete disclosure of product properties, a structure of pastille quality indicators has been developed – a «tree of properties». Thus, the comprehensive assessment of the quality of the control sample corresponds to the indicator «good» (0.66), while the integrated assessment of pastille with multicomponent paste corresponds to the indicator «very good» (0.98). On the basis of organoleptic, physicochemical indicators and chemical composition of products, it was established that the indicator of a comprehensive assessment of the quality of a pastille with a multicomponent paste, taking into account all group indicators, is 48 % higher compared to the control.


2020 ◽  
pp. 35-46
Author(s):  
Konstantin Grigor'yevich Bogolitsin ◽  
Anastasiya Eduardovna Parshina ◽  
Anna Sergeyevna Druzhinina ◽  
Elena Valer'yevna Shulgina

Brown algae are a valuable source of a variety of biologically active compounds. Their accumulation is influenced by many environmental factors in which macrophytes grow. The purpose of this study is to carry out comparative studies of the general chemical composition of brown algae in the White and Yellow Seas to substantiate the possibility of their use as raw materials for obtaining new pharmaceutical substances. Using the proposed scheme, which involves sequential supercritical fluid, acid, alkaline and water extraction, it was possible to isolate various components of brown algae, including a protein-polysaccharide complex, which in the case of the species Laminaria digitata and Laminaria saccharina is more than 80% cellulose and squirrel. The data obtained by FTIR spectroscopy confirm the qualitative composition of the complexes and also indicate its purity. Significant differences were revealed in the chemical elemental and component composition, which are affected by the growing conditions of macrophytes. The resulting chemical compounds from brown algae biomass, in particular the protein-polysaccharide complex, have a high potential for obtaining new pharmacological preparations based on them for health protection purposes. Based on the previously obtained data, it can be assumed that this complex has a double activity – enterosorption and immunomodulatory.


2021 ◽  
pp. 57-63
Author(s):  
И.Ю. Постраш

Трава зверобоя является ценным лекарственным растением, так как содержит большое число разнообразных биологически активных соединений, важнейшими из которых являются флавоноиды (1,5–5%), нафтодиантроны, дубильные вещества (10–13%), эфирное масло (0,1–0,33%) и др. Препараты, содержащие зверобой, комплексно воздействуют на организм, они оказывают противовоспалительное, антимикробное, антидепрессивное, противовирусное, кровоостанавливающее действие. Трава зверобоя применяется в виде чаёв, отваров, водных настоев, спиртовых и масляных настоек, мазей, сухих экстрактов. В ветеринарной медицине широко применяют настои и отвары травы зверобоя при заболеваниях ЖКТ: диспепсиях, гастроэнтеритах, острых и хронических колитах (КРС, овцы, козы, свиньи, собаки и др.), а также при лечении смешанных инвазий. Настои и настойку применяют также наружно при стоматитах, гингивитах, фарингитах. Несмотря на перечисленный выше ассортимент лекарственных форм зверобоя, активно ведутся научные исследования по оптимизации способов извлечения биологически активных веществ (БАВ) из травы зверобоя продырявленного. В ходе экспериментов исследователи варьируют различные параметры процесса экстракции: химический состав экстрагентов (монофазная и двухфазная экстракция), время и температуру экстракционного процесса, количественное соотношение сырья и экстрагентов, степень измельчения сырья, последовательность технологических операций, возможность применения излучения и др. Разработки новых ресурсосберегающих технологий переработки зверобоя продырявленного будут способствовать снижению себестоимости фармацевтической продукции и улучшению её фармакологических свойств. Hypericum perforatum grass is a valuable medicinal plant, since it contains a large number of various biologically active compounds, the most important of which are flavonoids (1.5–5%), naphthodianthrones, tannins (10–13%), aethereal oil (0.1–0.33%), etc. Preparations containing Hypericum perforatum have a complex effect on the body, they have an anti-inflammatory, antimicrobial, antidepressant, antiviral, hemostatic effect. Hypericum perforatum grass is used in the form of teas, apozems, water infusions, alcohol and oil tinctures, ointments, dry extracts. In veterinary medicine, infusions and decoctions of Hypericum perforatum grass are widely used in diseases of the gastrointestinal tract: dyspeptic disorder, gastroenteritis, acute and chronic colitis (cattle, sheep, goats, pigs, dogs, etc.), as well as in the treatment of mixed invasions. Infusions and tincture are also used externally for stomatitis, gingivitis, blennisthmia. Despite the above range of dosage forms of Hypericum perforatum, researches are actively conducted to optimize methods of extracting biologically active substances (BAS) from Hypericum perforatum grass. During the experiments, researchers vary various parameters of the extraction process: the chemical composition of extragents (monophasic and two-phase extraction), the time and temperature of the extractive process, the quantitative relation of raw materials to extragents, the dispersity of raw materials, the sequence of technological operations, the possibility of using radiation, etc. The development of new resource-saving technologies for processing Hypericum perforatum will help reduce the cost of pharmaceutical products and improve its pharmacological properties.


2019 ◽  
pp. 95-101
Author(s):  
Anatoliy Valeriyevich Velikorodov ◽  
Vladimir Nikolayevich Pilipenko ◽  
Tat'yana Alekseyevna Pilipenko ◽  
Sergey Viktorovich Malyi

The chemical composition of Prangos odontalgica (Pall.) Herrnst. et. Heyn essential oil, which grows wild in the Astrakhan region, has not been practically studied, unlike other species of the genus Prangos, growing in Iran, Turkey and Uzbekistan. To obtain the essential oil, all parts of the plant (roots, stems, flowers, leaves, fruits) are used, as well as various methods of isolation (hydrodistillation, supercritical fluid extraction, solid-phase microextraction, and others). Essential oil from the fruit of Prangos odontalgica we obtained by the method of hydrodistillation. The duration of the hydrodistillation process, established experimentally on the basis of a study of the dynamics of change in the yield of essential oil over time, was 3 hours. The yield of essential oil in mass% by volume in terms of absolutely dry raw materials according to the results of a threefold determination is 0.07 ± 0.01%. The method of gas-liquid chromatography carried out a quantitative analysis of the main components of the essential oil Prangos odontalgica. The quantitative content of the components of the essential oil was calculated by the areas of gas chromatographic peaks without the use of correction factors. Qualitative analysis was performed by comparing linear retention indices. In the Prangos odontalgica essential oil, 38 substances were identified (88.4%). It contains sesquiterpenes (31.48%), terpenoids (19.2%), hydrocarbons (15.39%), carboxylic acids and their esters (10.86%), alcohols, phenols (8.84%). Among sesquiterpenes, the predominant components are γ-elemen (9.84%), bisabolol (9.41%), and among terpenoids – trans-nerolidol (3.90%) and linalyl isobutyrate (3.41%).


Author(s):  
A. K. Kamysbaevа ◽  
◽  
G. E. Azimbaeva ◽  

Currently, 40% of drugs used in modern medicine are obtained from plant raw materials. Among the wild flora there are medicinal plant species containing a variety of chemical composition, including biologically active substances with antimicrobial activity and phytoncidal properties. This article presents biologically active substances of some species of the genus Asteraceae . The chemical composition of plants belonging to the Asteraceae family is represented by anthocyanins, inulins, pectins, flavonoids, proteins, cloves, fatty oils, coumarins, carotenes and polyphenols. The plant of the Asteraceae family is used in folk medicine as an anti-inflammatory, antitumor and cardiotonic agent.


Author(s):  
Gennady Ivanovich Kasyanov ◽  
Arthur Magomedovich Magomedov ◽  
Svetlana Vasilievna Zolotokopova

The article presents the results of technological and merchandising research on the development of formulations of frozen semi-finished products from sweet pepper stuffed with minced fish, bulgur, quinoa and couscous, enriched by CO2-extracts with spices and smoking preparation. Feasibility of making frozen semi-finished products from fish is confirmed by an increase in consumer demand for these products, besides, shock freezing of semi-finished products with liquid nitrogen helps to preserve the most valuable components of the original animal and plant raw materials. The technology of cooking stuffed pepper from zoned raw materials is presented: sweet pepper sorts “Pride of Russia”, “Yellow Bull”, “Topolyn” grown in the open ground of the environmentally friendly Novopokrovsky district of the Krasnodar region by experts of the company “Vegan Line”, and muscle tissue of grass carp migrated from the Far East, acclimatized in the water bodies of Kuban and the Astrakhan region. For the first time in technological practice there the wheat grain modified products - bulgur and couscous with biologically active substances have been added to minced meat. A detailed analysis of chemical composition of pepper sorts mixture and grass carp caught in the Anapa district of the Krasnodar region was performed. In the course of studying the chemical composition of bulgur, quinoa and couscous there was stated a high content of alible proteins, vitamins, macro- and microelements. There have been formulated three recipes of stuffed sweet pepper of yellow, green and red colors: with bulgur, couscous and quinoa. A hardware-technological scheme has been developed for producing semi-finished sweet pepper stuffed with minced fish, cereals and CO2-extracts of spices and smoke preparation. There has been analyzed the chemical composition of the finished semi-finished products, provides information about the nutritional and biological value of the product, indicating a higher content of basic food and biologically active substances.


2018 ◽  
Vol 14 (1-2) ◽  
pp. 18-23 ◽  
Author(s):  
L.V. Natrus ◽  
O.I. Kryvosheyeva ◽  
G.R. Lamazyan ◽  
T.S. Bruzgina

Relevance. The search for new forms of herbal medicine is an important area of modern biotechnology, pharmacology and pharmacy. More and more attention is paid to the development of extracts from plant materials, since the extraction products provide the maximum content of biologically active substances, which increases the rationality of their use, due to the accuracy of dosing, the therapeutic effect is increased, the period of use increases and storage conditions are simplified. The extraction of the fruit mixture of Citrullus сolocynthis in the Soxhlet apparatus was modified by reducing the procedure time 5-10 times and improving the processing of raw materials by using a press at the same time and increasing the temperature, or even by modifying the apparatus. Objective: to study the composition of FA in an aqueous solution of the dry extract of Citrullus сolocynthis when modifying the extraction mixture in the Soxhlet apparatus by increasing the temperature and technological reduction of processing time. Materials and methods. We carried out the determination and comparison of the composition of FA’s by gas-liquid chromatography of all parts of the fruit of Citrullus сolocynthis and in an aqueous solution of its dry extract with various concentrations of the substance. Results. Chromatographic analysis showed that all parts of the fetus Citrullus colocynthis are promising sources of saturated and unsaturated FA. In all parts of the fruit of Citrullus colocynthis, linoleic acid prevails in quantitative content. The modification of the extraction of the mixture in the Soxhlet apparatus by increasing the temperature and technological reduction in the processing time of the raw materials led to the production of a substance with a new composition and FA content. The ratio of unsaturated / saturated FA in the resulting dry extract is 5 times greater than the similar ratio separately in seeds, pulp and shell. At the same time, the distribution of separate unsaturated and polyunsaturated FA’s in the dry extract solution is fairly uniform. The relative amount of linoleic FA in the dry extract was smaller, and the linolenic FA was greater than in the seeds and pulp of the fruit. Conclusions. Modifying the extraction of the mixture in the Soxhlet apparatus by increasing the temperature and technological reduction of the processing time did not lead to complete degreasing of the substrate and allowed to obtain a dry extract of Citrullus solvent with content of microdoses of the FA, their balanced (uniform) in amount, and predominance of 5 times the amount of unsaturated polyunsaturated FA over the amount of saturated. We assume that such a redistribution of FA, and especially their micro doses, can be the basis for creating medicines with more effective effects on the body.


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