scholarly journals CHITOSAN INHIBITION TEST AGAINST E. coli AND DIGESTIBILITY OF THE RATION IN THE IN-VITRO METHOD

2020 ◽  
Vol 11 (2) ◽  
pp. 230
Author(s):  
Eli Sahara ◽  
Sofia Sandi ◽  
Fitra Yosi
Keyword(s):  
Escherichia Coli ◽  
Crude Protein ◽  
Dry Matter ◽  
Control Treatment ◽  
Clear Zone ◽  
Bacterial Colony ◽  
E Coli ◽  
Chicken Feces ◽  
Inhibitory Power

Diarrhea and vomiting are often caused by E coli bacteria. E coli bacteria has a strain of Shigatoxigenic Escherichia coli (STEC), producing Shiga poisons or poisons such as Shiga (verotoxin) which are harmful and pollute nature. This strain of the E coli bacterium has a detrimental effect because it excludes one or both types of Shiga Like Toxin -1 (Stx -1) and Shiga Like Toxin-2 (Stx-2) toxins. This bacterial infection has the potential as a zoonotic agent because it has been found in feces and sheep meat, feces and beef meat, chicken feces and human feces. If this bacterial colony inceases in the digestive tract of poultry it will disturb the productivity of the livestock.  Therefore it must be watched out and studied more deeply. The objectives of the study are 1) to see the inhibitory power of chitosan on the growth and development of E coli bacteria in vitro 2) the test of digestibility of dry matter (BK) and crude protein (PK) ration in vitro. The treatments given in this test are: R0 = control (without chitosan), R1 = 0.5% chitosan, R2 = 1% chitosan, R3 = 1.5% chitosan, R4 = 2% chitosan, R5 = 2.5% chitosan. The parameters measured were 1) inhibition of chitosan against E. coli growth based on clear zone diameter 2) digestibility of dry matter (BK) and crude protein (PK) ration in vitro. The results showed that the higher level of chitosan administration showed greater inhibition, which was indicated by the greater diameter of the clear zone caused. The provision of 2.5% chitosan shows medium inhibition that is has a range of 10-14 mm. The addition of a dose of 1.5% chitosan in the ration was able to increase the digestibility of dry matter by 7.86% and the digestibility of crude protein 11.20% higher than the control treatment (without chitosan). The conclusion of this study is that chitosan can inhibit the growth of E coli and improve the digestibility of dry matter (BK) and crude protein (PK) for the better.AbstrakPenyakit diare dan muntah-muntah sering disebabkan oleh bakteri E coli.  Bakteri E coli memiliki strain Shigatoxigenic Escherichia coli (STEC), menghasilkan racun Shiga atau  racun seperti Shiga (verotoxin) yang  berbahaya dan mencemari  alam. Strain dari bakteri E coli  ini mempunyai efek merugikan karena mengeluarkan salah satu atau kedua jenis toxin Shiga Like Toxin -1 (Stx -1) maupun Shiga Like Toxin-2 (Stx-2).  Infeksi bakteri ini berpotensi sebagai agen zoonosis karena sudah pernah ditemukan pada feses dan daging domba, feses dan daging sapi serta feses ayam dan feses manusia.  Jika koloni bakteri ini tinggi dalam saluran pencernaan unggas akan mengganggu produktivitas ternak tersebut.  Oleh sebab itu harus diwaspadai dan dikaji lebih mendalam. Tujuan penelitian adalah 1) melihat daya hambat kitosan terhadap pertumbuhan dan perkembangan bakteri E coli secara in vitro 2) menguji kecernaan bahan kering (BK) dan protein kasar (PK) ransum secara in vitro. Perlakuan yang diberikan dalam pengujian ini adalah: R0 = kontrol (tanpa kitosan), R1 = 0,5% kitosan, R2 = 1 % kitosan, R3 = 1,5% kitosan, R4 = 2% kitosan, R5 = 2,5% kitosan.   Parameter yang diukur adalah 1) daya hambat kitosan terhadap pertumbuhan E. coli berdasarkan diameter zona bening (in vitro) 2) kecernaan bahan kering (BK) dan protein kasar (PK) ransum secara in vitro. Hasil penelitian menunjukkan bahwa semakin tinggi level pemberian kitosan menunjukkan daya hambat yang semakin besar yang ditandai oleh semakin besarnya diameter zona bening yang ditimbulkan. Pemberian 2,5% kitosan menunjukkan daya hambat sedang yaitu memiliki range 10 - 14 mm. Penambahan dosis 1,5% kitosan dalam ransum, mampu meningkatkan kecernaan bahan kering 7,86% dan kecernaan protein kasar 11,20% lebih tinggi dari perlakuan kontrol (tanpa kitosan). Kesimpulan penelitian ini adalah bahwa kitosan mampu menghambat pertumbuhan E coli dan meningkatkan kecernaan bahan kering (BK) serta protein kasar (PK) menjadi lebih baik.Kata kunci: Kitosan, daya hambat, E. coli, kecernaan ransum, in vitro

scholarly journals Bromatological composition and dry matter digestibility of millet cultivars subjected to nitrogen doses

2014 ◽  
Vol 66 (3) ◽  
pp. 887-893 ◽  
Author(s):  
W.H.D. Buso ◽  
A.F.S. França ◽  
E.S. Miyagi
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Block Design ◽  
Control Treatment ◽  
Average Height ◽  
Dry Matter Digestibility ◽  
Acid Detergent Fibre ◽  
N Dose ◽  
Sowing Season

The bromatological composition and in vitro dry matter digestibility of millet cultivars were assessed for different nitrogen doses and two sowing seasons in the Ceres municipality of Goiás state, Brazil. The treatments consisted of three millet cultivars (ADR-7010, ADR-500 and BRS-1501), four nitrogen (N) doses (0, 50, 100 and 200kg ha-1 of N) and two sowing seasons. Three replicates and a randomised block design with a 3 x 4 x 2 factorial scheme were used. Two cuttings were performed in each season when plants reached an average height of 0.70 cm. No significant interactions were observed between or among cultivars for N doses and dry matter (DM), crude protein (CP), neutral detergent fibre (NDF) and acid detergent fibre (ADF) contents. The DM, CP, NDF and ADF contents were significantly different between N doses. The DM and CP contents increased as the N dose increased to 100kg ha-1. The maximum DM and CP contents were 11.14 and 22.53%, respectively. The NDF and ADF contents were higher in the control treatment (60.11 and 30.01%, respectively). In addition, the lowest ADF and NDF concentrations occurred at an N dose of 50kg ha-1(56.33 and 30.23%, respectively). The DM contents were higher for the February sowing, with an average of 10.59%. The highest CP and ADF contents were found for the December sowing (22.46 and 31.58%, respectively). No significant differences were found for millet cultivars, N doses or sowing seasons. A significant interaction was found between sowing season and millet cultivar. The BRS-1501 cultivar had a higher in vitro dry matter digestibility in the December/2010 sowing (73.88%).

Reduction of Escherichia coli O157:H7 and Salmonella on Laboratory-Inoculated Alfalfa Seed with Commercial Citrus-Related Products†

2003 ◽  
Vol 66 (7) ◽  
pp. 1158-1165 ◽  
Author(s):  
WILLIAM F. FETT ◽  
PETER H. COOKE
Keyword(s):  
Escherichia Coli ◽  
Tap Water ◽  
The United States ◽  
Control Treatment ◽  
In Vitro Assays ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Alfalfa Seed ◽  
Alfalfa Sprouts

Alfalfa sprouts contaminated with the bacterial pathogens Escherichia coli O157:H7 and Salmonella have been the source of numerous outbreaks of foodborne illness in the United States and in other countries. The seed used for sprouting appears to be the primary source of these pathogens. The aim of this study was to determine whether the efficacy of commercial citrus-related products for sanitizing sprouting seed is similar to that of high levels of chlorine. Five products (Citrex, Pangermex, Citricidal, Citrobio, and Environné) were tested at concentrations of up to 20,000 ppm in sterile tap water and compared with buffered chlorine (at 16,000 ppm). Alfalfa seeds were inoculated with four-strain cocktails of Salmonella and E. coli O157:H7 to give final initial concentrations of ca. 9.0 and 7.0 CFU/g, respectively. Treatments (10 min) with Citrex, Pangermex, and Citricidal at 20,000 ppm and chlorine at 16,000 ppm produced similar log reductions for alfalfa seed inoculated with four-strain cocktails of E. coli O157:H7 and Salmonella (3.42 to 3.46 log CFU/g and 3.56 to 3.74 log CFU/g, respectively), and all four treatments were significantly (P < 0.05) more effective than the control treatment (a buffer wash). Citrobio at 20,000 ppm was as effective as the other three products and chlorine against Salmonella but not against E. coli O157:H7. Environné was not more effective (producing reductions of 2.2 to 2.9 log CFU/g) than the control treatment (which produced reductions of 2.1 to 2.3 log CFU/g) against either pathogen. None of the treatments reduced seed germination. In vitro assays, as well as transmission electron microscopy, confirmed the antibacterial nature of the products that were effective against the two pathogens and indicated that they were bactericidal. When used at 20,000 ppm, the effective citrus-related products may be viable alternatives to chlorine for the sanitization of sprouting seed pending regulatory approval.

Evaluation of soil amendments in perennial ryegrass pastures associated with white and red clover in small-scale milk production systems

2020 ◽  
Vol 13 (11) ◽  
Author(s):  
Cloe Dafne Álvarez García ◽  
Carlos Manuel Arriaga Jordán ◽  
Julieta Gertrudis Estrada Flores ◽  
Felipe López González
Keyword(s):  
Perennial Ryegrass ◽  
Crude Protein ◽  
Dry Matter ◽  
Soil Amendments ◽  
Red Clover ◽  
Metabolizable Energy ◽  
Neutral Detergent Fiber ◽  
Control Treatment ◽  
Small Scale

Objective:  evaluate the effect of the inclusion of lime and dolomite soil amendments in an established pasture of perennial ryegrass (Lolium perenne) cv. Tetragrain associated with white clover (Trifolium repens) cv. Ladino and red clover (Trifolium pratense) cv. Kendall. Methodology: Three treatments of soil amendments were implemented on a perennial ryegrass with white and red clover pasture A split plot experimental design was used. The treatments were: T1= control treatment (without soil amendment), T2= lime and T3= dolomite. The evaluated variables were: herbage mass (HM), dry matter (DM), neutral detergent fiber (NDF), acid detergent fiber (ADF), crude protein (CP), in vitro dry matter digestibility (IVDMD) and estimated metabolizable energy (eME). Results: There were significant differences in all evaluated variables, the interaction between experimental periods and treatments was not significant for any variable. The treatments did not affect herbage mass. As for the the soil, a decrease in pH is observed at the end of the experiment, and there was an increase in the total soluble calcium, magnesium and nitrogen and a decrease in aluminum. Implications: This study illustrates the use of soil amendments, which somehow modulate effects of the farmer practices on the pasture soils. Conclusions: Inclusion of soil amendments in pastures did not increase dry matter production, content of FDN, FDA or IVDMD, but increased crude protein and evaluated minerals, and decreases aluminum.

scholarly journals UJI AKTIFITAS ANTIBAKTERI EKSTRAK ETANOL KULIT BUAH MANGGIS (Gracinia mangostana L.) TERHADAP PERTUMBUHAN BAKTERI Escherichia coli

2018 ◽  
pp. 7
Author(s):  
Putri Permata ◽  
Retno Kawuri ◽  
AA Ketut Darmadi
Keyword(s):  
Escherichia Coli ◽  
Ethanol Extract ◽  
Human Colon ◽  
In Vitro Test ◽  
Clear Zone ◽  
Garcinia Mangostana ◽  
Body Tissues ◽  
Vitro Test ◽  
Inhibitory Power

Mangosteen rind has a benefical compounds liked xanthone which consisting of mangostin, mangosterol, mangostinon A and B, trapezifolixanthone, tovophyllin B, alpha and beta mangostin, garcinon B, mangostanol, gartanin, and flavonoid epikatekin that known contain an antibacterial and antidiare agent. Escherichia coliis an opportunistic bacterium that is commonly found in the human colon as a normal flora. Its unique because it can cause primary infection in the intestine such as diarrhea in children, as well as its ability to cause infection in other body tissues outside the intestine.This research was done from December 2016 to March 2017. The methods that used in this research were well diffusion test to know the inhibitory power by measuring the clear zone formed. measure the clear zone of mangosteen rind against Escherichia coli. Then to testing extract compounds, there was used phytochemicals screening test. Data there has been found will be analyzed through analysis of variance (ANOVA). In the inhibitory test of ethanol extract from mangosteen rind (G. mangostana L.), it was so effective to inhibit the growth of Escherichia coli through in-vitro test with a resistivity of 1,58 cm and categorized as a strong inhibitory, which MIC from mangosteen rind against Escherichia coli was 3,9% and the compounds that contain in ethanol extract from mangosteen rind (G. mangostana L.) were alkaloid, phenolik, flavonoid, saponin and terpenoid. Key word : antibacterial activity, Garcinia mangostana L., Escherichia coli.

Substituted 4-Formyl-2H-chromen-2-ones: Their Reaction with N-(2,3,4,6-Tetra-Oacetyl- β-D-galactopyranosyl)thiosemicarbazide, Antibacterial and Antifungal Activity of Their Thiosemicarbazone Products

2020 ◽  
Vol 24 (19) ◽  
pp. 2272-2282
Author(s):  
Vu Ngoc Toan ◽  
Nguyen Minh Tri ◽  
Nguyen Dinh Thanh
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Selenium Dioxide ◽  
Antibacterial And Antifungal Activity ◽  
E Coli ◽  
Antibacterial And Antifungal Activities ◽  
Alkyl Ethers ◽  
Antibacterial And Antifungal

Several 6- and 7-alkoxy-2-oxo-2H-chromene-4-carbaldehydes were prepared from corresponding alkyl ethers of 6- and 7-hydroxy-4-methyl-2-oxo-2H-chromen-2-ones by oxidation using selenium dioxide. 6- and 7-Alkoxy-4-methyl-2H-chromenes were obtained with yields of 57-85%. Corresponding 4-carbaldehyde derivatives were prepared with yields of 41-67%. Thiosemicarbazones of these aldehydes with D-galactose moiety were synthesized by reaction of these aldehydes with N-(2,3,4,6-tetra-O-acetyl-β-Dgalactopyranosyl) thiosemicarbazide with yields of 62-74%. These thiosemicarbazones were screened for their antibacterial and antifungal activities in vitro against bacteria, such as Staphylococcus aureus, Escherichia coli, and fungi, such as Aspergillus niger, Candida albicans. Several compounds exhibited strong inhibitory activity with MIC values of 0.78- 1.56 μM, including 8a (against S. aureus, E. coli, and C. albicans), 8d (against E. coli and A. niger), 9a (against S. aureus), and 9c (against S. aureus and C. albicans).

scholarly journals Evaluation of the Nutrient Composition, In Vitro Fermentation Characteristics, and In Situ Degradability of Amaranthus caudatus, Amaranthus cruentus, and Amaranthus hypochondriacus in Cattle

Animals ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 18
Author(s):  
Kim Margarette C. Nogoy ◽  
Jia Yu ◽  
Young Gyu Song ◽  
Shida Li ◽  
Jong-Wook Chung ◽  
...  
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Gas Production ◽  
Nutrient Composition ◽  
Neutral Detergent Fiber ◽  
Amaranthus Cruentus ◽  
Amaranthus Caudatus ◽  
Amaranthus Hypochondriacus

The amaranth plants showed high potential feed value as forage for ruminants. An in-depth study of this plant, particularly in cattle, will help extend its utilization as an alternative protein and fiber feed source in cattle feeding. In this study, the nutrient compositions of three different species of amaranth, Amaranthus caudatus L., Amaranthus cruentus L., and Amaranthus hypochondriacus L.—two varieties for each species, A.ca 74, A.ca 91, A.cu 62, A.cu 66, A. hy 30, and A. hy 48—were evaluated. The in vitro technique was used to evaluate the fermentation characteristics such as total gas production, total volatile fatty acids (VFA) concentration, pH, and ammonia concentration of the rumen fluid. Moreover, the effective degradabilities of dry matter (EDDM) and crude protein (EDCP) of the amaranth forages were determined through in situ bag technique. The amaranth forages: A. caudatus, A. cruentus, and A. hypochondriacus showed better nutritive value than the locally produced forages in Chungcheong province of Korea. The CP of the amaranth ranged from 11.95% to 14.19%, and the neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents ranged from 45.53% to 70.88% and 34.17% to 49.83%, respectively. Among the amaranth varieties, A. hypochondriacus 48 showed the most excellent ruminant feed nutrient quality (CP, 14.19%; NDF, 45.53%; and ADF, 34.17%). The effective degradabilities of dry matter (EDDM; 33–56%) and crude protein EDCP (27–59%) of the amaranth were lower compared to other studies, which could be due to the maturity stage at which the forages were harvested. Nonetheless, A. hypochondriacus 48 showed the highest EDDM (56.73%) and EDCP (59.09%). The different amaranth species did not differ greatly in terms of total VFA concentration or molar proportions, total gas production, or ammonia-N concentration. The high nutrient composition, and highly effective degradability of dry matter and crude protein, coupled with the favorable fermentation characteristics, suggest that the amaranth forages showed good to excellent feed quality for cattle.

scholarly journals In vitro activity of antimicrobial peptide CDP-B11 alone and in combination with colistin against colistin-resistant and multidrug-resistant Escherichia coli

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kaitlin S. Witherell ◽  
Jason Price ◽  
Ashok D. Bandaranayake ◽  
James Olson ◽  
Douglas R. Call
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Peptide ◽  
Membrane Integrity ◽  
Antibiotic Resistance Genes ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Multidrug Resistant Bacteria ◽  
E Coli ◽  
Minimal Media

AbstractMultidrug-resistant bacteria are a growing global concern, and with increasingly prevalent resistance to last line antibiotics such as colistin, it is imperative that alternative treatment options are identified. Herein we investigated the mechanism of action of a novel antimicrobial peptide (CDP-B11) and its effectiveness against multidrug-resistant bacteria including Escherichia coli #0346, which harbors multiple antibiotic-resistance genes, including mobilized colistin resistance gene (mcr-1). Bacterial membrane potential and membrane integrity assays, measured by flow cytometry, were used to test membrane disruption. Bacterial growth inhibition assays and time to kill assays measured the effectiveness of CDP-B11 alone and in combination with colistin against E. coli #0346 and other bacteria. Hemolysis assays were used to quantify the hemolytic effects of CDP-B11 alone and in combination with colistin. Findings show CDP-B11 disrupts the outer membrane of E. coli #0346. CDP-B11 with colistin inhibits the growth of E. coli #0346 at ≥ 10× lower colistin concentrations compared to colistin alone in Mueller–Hinton media and M9 media. Growth is significantly inhibited in other clinically relevant strains, such as Acinetobacter baumannii, Pseudomonas aeruginosa, and Klebsiella pneumoniae. In rich media and minimal media, the drug combination kills bacteria at a lower colistin concentration (1.25 μg/mL) compared to colistin alone (2.5 μg/mL). In minimal media, the combination is bactericidal with killing accelerated by up to 2 h compared to colistin alone. Importantly, no significant red blood hemolysis is evident for CDP-B11 alone or in combination with colistin. The characteristics of CDP-B11 presented here indicate that it can be used as a potential monotherapy or as combination therapy with colistin for the treatment of multidrug-resistant infections, including colistin-resistant infections.

scholarly journals Lysyl-tRNA synthetase from Escherichia coli K12. Chromatographic heterogeneity and the lysU-gene product

1987 ◽  
Vol 248 (1) ◽  
pp. 43-51 ◽  
Author(s):  
J Charlier ◽  
R Sanchez
Keyword(s):  
Escherichia Coli ◽  
Gene Product ◽  
Activity Ratio ◽  
Deae Cellulose ◽  
Trna Synthetase ◽  
Trna Synthetases ◽  
E Coli ◽  
Aminoacyl Trna Synthetases

In contrast with most aminoacyl-tRNA synthetases, the lysyl-tRNA synthetase of Escherichia coli is coded for by two genes, the normal lysS gene and the inducible lysU gene. During its purification from E. coli K12, lysyl-tRNA synthetase was monitored by its aminoacylation and adenosine(5′)tetraphospho(5′)adenosine (Ap4A) synthesis activities. Ap4A synthesis was measured by a new assay using DEAE-cellulose filters. The heterogeneity of lysyl-tRNA synthetase (LysRS) was revealed on hydroxyapatite; we focused on the first peak, LysRS1, because of its higher Ap4A/lysyl-tRNA activity ratio at that stage. Additional differences between LysRS1 and LysRS2 (major peak on hydroxyapatite) were collected. LysRS1 was eluted from phosphocellulose in the presence of the substrates, whereas LysRS2 was not. Phosphocellulose chromatography was used to show the increase of LysRS1 in cells submitted to heat shock. Also, the Mg2+ optimum in the Ap4A-synthesis reaction is much higher for LysRS1. LysRS1 showed a higher thermostability, which was specifically enhanced by Zn2+. These results in vivo and in vitro strongly suggest that LysRS1 is the heat-inducible lysU-gene product.

scholarly journals A Rhodobacter sphaeroides Protein Mechanistically Similar to Escherichia coli DksA Regulates Photosynthetic Growth

mBio ◽  
2014 ◽  
Vol 5 (3) ◽  
Author(s):  
Christopher W. Lennon ◽  
Kimberly C. Lemmer ◽  
Jessica L. Irons ◽  
Max I. Sellman ◽  
Timothy J. Donohue ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Structure Function ◽  
Rhodobacter Sphaeroides ◽  
Fatty Acid Content ◽  
Regulatory Protein ◽  
Growth Conditions ◽  
Globular Domain ◽  
Content Type ◽  
E Coli

ABSTRACTDksA is a global regulatory protein that, together with the alarmone ppGpp, is required for the “stringent response” to nutrient starvation in the gammaproteobacteriumEscherichia coliand for more moderate shifts between growth conditions. DksA modulates the expression of hundreds of genes, directly or indirectly. Mutants lacking a DksA homolog exhibit pleiotropic phenotypes in other gammaproteobacteria as well. Here we analyzed the DksA homolog RSP2654 in the more distantly relatedRhodobacter sphaeroides, an alphaproteobacterium. RSP2654 is 42% identical and similar in length toE. coliDksA but lacks the Zn finger motif of theE. coliDksA globular domain. Deletion of the RSP2654 gene results in defects in photosynthetic growth, impaired utilization of amino acids, and an increase in fatty acid content. RSP2654 complements the growth and regulatory defects of anE. colistrain lacking thedksAgene and modulates transcriptionin vitrowithE. coliRNA polymerase (RNAP) similarly toE. coliDksA. RSP2654 reduces RNAP-promoter complex stabilityin vitrowith RNAPs fromE. coliorR. sphaeroides, alone and synergistically with ppGpp, suggesting that even though it has limited sequence identity toE. coliDksA (DksAEc), it functions in a mechanistically similar manner. We therefore designate the RSP2654 protein DksARsp. Our work suggests that DksARsphas distinct and important physiological roles in alphaproteobacteria and will be useful for understanding structure-function relationships in DksA and the mechanism of synergy between DksA and ppGpp.IMPORTANCEThe role of DksA has been analyzed primarily in the gammaproteobacteria, in which it is best understood for its role in control of the synthesis of the translation apparatus and amino acid biosynthesis. Our work suggests that DksA plays distinct and important physiological roles in alphaproteobacteria, including the control of photosynthesis inRhodobacter sphaeroides. The study of DksARsp, should be useful for understanding structure-function relationships in the protein, including those that play a role in the little-understood synergy between DksA and ppGpp.

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