scholarly journals Experimental investigation of peculiarities for impact of a sorption action preparation silicon dioxide on infectious agents of cholangitis

2021 ◽  
Vol 88 (3-4) ◽  
pp. 80-86
Author(s):  
O. I. Dronov ◽  
I. O. Kovalska ◽  
I. L. Nastashenko ◽  
T. O. Kondratiuk ◽  
L. V. Levchenko
Keyword(s):  
Silicon Dioxide ◽  
Acute Cholangitis ◽  
Bactericidal Effect ◽  
E Coli ◽  
Combined Application ◽  
National University ◽  
Action Preparation ◽  
Biliary Ducts ◽  
The Impact ◽  
Test Cultures

Objective. Determination of impact of combination, consisted of 3% solution of silicon dioxide and antibacterial preparations, on bacterial cultures of S. aureus, P. aeruginosa, E. coli. Materials and methods. Clean cultures of bacteria, kept in Collection of Microorganisms in Department of Microbiology and Immunology of Training–Scientific Centre «Іnstitute of Biology and Medicine» of Taras Shevchenko National University: S. aureus (Rosenbach, 1884), P. aeruginosa (Schröter, 1872; Migula, 1900), E. coli (Migula, 1895; Castellani і Chalmers, 1919) were applied. Action of antibacterial preparations cefoperazon, cefoperazon–sulbactam, ampicillin–sulbactam, amicacin, as well as enterosorbent 3% solution of silicon dioxide, was investigated. In the investigation the impact of antibacterial preparations and silicon dioxide together with antibacterial preparations on test–cultures of bacteria was studied, using broth and agarised medium of Muller–Hinton. Results. The data, obtained in the investigation, trust efficacy of combined application of 3% solution of silicon dioxide, owing sorption capacity, with antibacterial preparations in reduction or elimination of subsequent growth of bacterial test–cultures of S. aureus, P. aeruginosa, E. coli. Combination of 3% solution of silicon dioxide with ampicillin–sulbactam in dilutions 64 and 32 mg/l promotes reduction of the E. coli growth on surface of agarised medium in comparison with the investigation variant without application of 3% solution of silicon dioxide. While polyresistence of P. aeruginosa revealed, 3%solution of silicon dioxide together with cefoperazon–sulbactam in concentrations 8 and 16 mg/l and ceftazidime in concentrations 8, 16, 32, 64 mg/l guarantees bactericidal effect for this microorganism. Growth of P. aeruginosa while application of 3% solution of silicon dioxide in combination with ampicillin–sulbactam in dilutions 8, 16, 32, 64 mg/l was arrested. Application of 3% solution of silicon dioxide in combination with amicacin–sulbactam 16 mg/l inhibits the S. aureus growth, what is observed, when combination of 3% solution of silicon dioxide with amicacin in dilutions 8, 16 mg/l and cefoperazon–sulbactam in dilutions 8, 16 mg/l are exploited. Growth of the microorganism do not evolve in variants of the investigation without 3% solution of silicon dioxide, as well as with him, if amikacin is applied in concentrations 64 and 32 mg/l. Conclusion. The biliary ducts drainage in obturative jaundice of tumoral genesis and subsequent presence of drainage (internal or external) constitute the main risk factors for their colonization by multiresistant microorganisms. At the same time, the biliary ducts drainage in combination with antibioticotherapy is estimated as an urgent method for control of the infection primary focus in an acute cholangitis. Establishing of procedure for application of 3% solution of silicon dioxide in combination with certain groups of antibacterial preparations for microorganisms revealed constitute important measure for bacteriobilia prophylaxis. The further investigations conduction with wider spectrum of antibacterial preparations and 3% solution of silicon dioxide for control of clinically significant species of microorganisms is necessary and perspective.

scholarly journals RESISTANCE OF S. AUREUS ATCC 25923, E. COLI 055K59 No. 3912/41 AND P. AERUGINOSA 27/99 TO THE WASH-DISINFECTANT «MILKODEZ»

2020 ◽  
Vol 1 ◽  
pp. 55-60
Author(s):  
Mykola Verkholyuk ◽  
Ruslan Peleno ◽  
Iaromyr Turko
Keyword(s):  
Optical Density ◽  
Ethyl Alcohol ◽  
Standard Test ◽  
Hot Water ◽  
E Coli ◽  
Microbial Biofilms ◽  
Planktonic Form ◽  
Petri Dishes ◽  
The Impact ◽  
Test Cultures

The aim of the work – the article presents the results of determining of the resistance of S. aureus ATCC 25923, E. coli 055K59 No. 3912/4 and P. aeruginosa 27/99 test cultures in planktonic form and in biofilm to our developed «Milkodez» acid detergent. Materials and methods. Microbial biofilms were grown on MPB in 5 cm disposable plastic Petri dishes. To determine the effect of disinfectants on microbial biofilms, 3 Petri dishes with biofilms of each of the test cultures were used. One of the Petri dishes served as control and she had for 15 minutes made 5 cm3 of saline NaCl solution, in the second – 5 cm3 of hot water (t=70±5 °C), and in the third – 5 cm3 of acidic detergent «Milkodez». Microbial biofilms were fixed for 10 min. 96º with ethyl alcohol for 10 min. were stained with a 0.1 % solution of crystalline violet, and the remnants of the unabsorbed paint were removed with phosphate buffer. The biofilm dye was extracted with 96º of ethyl alcohol, which was photocolometrically investigated at 570 nm to establish the density of the formed biofilms. The density of the formed microbial biofilms was considered low in optical density of the extract up to 0.5 units, average – from 0.5 to 1.0 units; and high – over 1.0 units The resistance of planktonic forms of test cultures of microorganisms to disinfectants was determined in sterile tubes, which made 10 cm3 (t=70±5 °C) of 0.5 % of their working solutions and 0.1 cm3 (1 billion microbial bodies) of the standard test – cultures. The culture was maintained for 15 min. and made ten – fold plantings on IPA in Petri dishes. Incubation of mesophilic microorganisms was carried out in a thermostat at a temperature of 30 °C, and psychrophilic – 20 °C. After 48 hours the calculation of the growing colonies were carried out. The results were expressed in colony forming units (CFU). Results. Due to the impact on microbial biofilms formed by the test cultures of S. aureus ATCC 25923, E. coli 055K59 No. 3912/41 and P. aeruginosa 27/99 for 15 min. 0.5 % solution of acid detergent «Milkodez» the optical density of the solutions was respectively 0.64, 0.72, 0.45 units. The results obtained indicate that the melkodez caused a decrease in the biofilm–forming ability of S. aureus ATCC 25923 3.2 times, in E. coli 055K59 No. 3912/41 – 1,7 times and in P. aeruginosa 27/99 – 2.8 times, compared to control. However, the density of one – day microbial biofilms formed by S. aureus ATCC 25923 and E. coli 055K59 No. 3912/41 was medium, and P. aeruginosa 27/99 was low. It has been proven that the «Milkodez» acid detergent developed is more effective than the prototype «Hypracid», since it caused the death of 100 % of planktonic test cultures and the number of S. aureus ATCC 25923, E. coli 055K59 No. 3912/41 and P. aeruginosa 27/99 formed in the biofilm that survived after its application was 2.7, 3.2 and 1.4 times lower, respectively. Conclusions. It was found that the test cultures were able to form high – density biofilms, since the optical density of the extract in the control was in the range from 1.28 to 2.05 units, which is greater than 1.0 units. Acid wash detergent «Milkodez» for 15 minutes of exposure causes the formation of S. aureus ATCC 25923, E. coli 055K59 No. 3912/41 and P. aeruginosa 27/99 biofilms of low and medium density and reduces their biofilm capacity by 3.2, 1.7 and 2.8 times, respectively. Its use provides the death of 100 % of the planktonic forms of the test cultures under study and reduces their number in the biofilm by 2.7, 3.2 and 1,4 times more, respectively, compared to «Hypracid» detergent.

scholarly journals Diagnostic and Therapeutic Value of ERCP in Acute Cholangitis

2013 ◽  
Vol 2013 ◽  
pp. 1-4 ◽  
Author(s):  
Kenan Buyukasik ◽  
Ahmet Burak Toros ◽  
Hasan Bektas ◽  
Aziz Ari ◽  
Mehmet Mehdi Deniz
Keyword(s):  
Bile Ducts ◽  
Acute Cholangitis ◽  
Complication Rates ◽  
Endoscopic Retrograde ◽  
Therapeutic Success ◽  
Mortality And Morbidity ◽  
E Coli ◽  
Therapeutic Value ◽  
Ascending Cholangitis ◽  
Biliary Ducts

Cholangitis, with a clinical spectrum between acute ascending cholangitis and acute fulminant cholangitis, the mildest and the most severe forms, respectively, is the infection of bile ducts with a potential of serious mortality and morbidity. Obstruction of the bile ducts followed by infection, with E. coli being the most commonly isolated agent, is common to all forms of cholangitis. Biliary obstruction is caused by choledocholithiasis mostly. “Choledochal pressure” is the most important factor, determining morbidity. If the pressure exceeds 25 cm H2O, which is the critical value, immune dysfunction ensues. Sepsis is common if the infection of biliary ducts is suppurative. Mortality and morbidity are inevitable if left untreated or drained late. The objective of this study is, in the stand point of the current literature, to analyse the diagnostic, therapeutic success and complication rates of ERCP (Endoscopic retrograde cholangiopancreatography) in patients with a diagnosis of acute purulent cholangitis with no response to medical treatment.

scholarly journals Use of ozone for dіcrease of microbal load

2020 ◽  
pp. 50-55
Author(s):  
M. Savcheniuk ◽  
B. Yarchuk ◽  
L. Korniienko ◽  
T. Tsarenko ◽  
D. Okhrimenko ◽  
...  
Keyword(s):  
Corona Discharge ◽  
Petri Dish ◽  
Bactericidal Effect ◽  
Operating Costs ◽  
Process Equipment ◽  
Main Method ◽  
E Coli ◽  
Pulsed Mode ◽  
Impact On Survival ◽  
Test Cultures

Ozone enters into chemical reaction with many organic compounds. Ozone is a very strong oxidant. It oxidizes most of the elements to higher oxides. In the oxidation reaction by ozone activity second only to fluorine, its oxide and free radicals. It is formed from oxygen by absorbing heat in this case, and, conversely, when the expansion goes into oxygen, giving off heat. The main method of obtaining ozone for practical purposes is electrosynthesis. In industrial conditions for using ozone and corona discharge barrier. Ozonizers corona discharge can significantly increase the efficiency of ozone by reducing capital and operating costs for the process equipment and ozonation. Research shows that ozone air ozonator pulsed mode increases the resistance of animals to the action of microorganisms factor. In animals and poultry in industrial production often develop diseases caused by pathogens factor. These diseases mainly occurring symptom of lesions of the respiratory and digestive systems. In many countries today conducted research using ozone, and the results of these studies are published in the course of scientific-metric database. For normal growing animals, especially young animals, premises should be thoroughly disinfected. Disinfection is required for all technological facilities as livestock and poultry. Experimentally found that ozone has a bactericidal effect (for example, test cultures E. coli) on different surfaces (wood, Petri dish) considering its concentration and exposure. The results of influence of different concentrations of ozone in ozonair mixture is fed into the container of the Petri dish, and time impact on survival of microorganisms provides a fairly accurate prediction of the results of impact parameters ozonation. Key words: ozon, ozone therapy, Escherichia coli, disinfection.

scholarly journals Activity of washing-disinfecting means “San-active” for sanitary treatment of equipment of meat processing enterprises in laboratory and manufacturing conditions

2018 ◽  
Vol 1 (1) ◽  
pp. 10-16
Author(s):  
V. Salata ◽  
M. Kukhtyn ◽  
Yu. Pekriy ◽  
Yu. Horiuk ◽  
V. Horiuk
Keyword(s):  
Pathogenic Bacteria ◽  
Active Agent ◽  
Bactericidal Effect ◽  
Hydrogen Ions ◽  
Meat Processing ◽  
Alkaline Ph ◽  
E Coli ◽  
Working Solution ◽  
Sanitary Treatment ◽  
Test Cultures

The article presents the results of the research of the new detergent agent “San-active” for meat processing enterprises. It was established that “San-active” in the concentration from 0.3 to 2.0% is moderately alkaline (concentration of hydrogen ions is 11.44–12.7), at a concentration of 2.5% and above, with very alkaline pH ≥ 13.11 units. In the “San-active” detergent, at the concentration from 0.3 to 2.5%, the surface tension is 34.97–28.24 mN/m. The absorbability of the parts of the technological equipment with the solutions of the “San-active” means sharply increases with increasing concentration. At the temperature of solutions of the medium 19.0 ± 1.0 °С the angle of wetting decreases from 69.8 degrees. at a concentration of 0.3% to 50.5 degrees. at a concentration of 2.5% (in 1.4 times). It has been established that “San-active” in 0.5% concentration provides the bactericidal effect on test cultures of conditionally pathogenic bacteria, spore-forming microorganisms and fungi. The “San-active” agent at 0.5% concentration is bactericidal to S. aureus and E. faecalis cells that are in a biofilm in 10 minutes of exposition. For the inactivation of E. coli and P. aeruginosa cells in a biofilm, it is necessary that the “San-active” acts in a concentration not lower than 0.5% and not less than 30 minutes. The agent shows a washing effect on the evaluation of “good” at 0.5% concentration, and 1.0% and above the concentration on the score “excellent”. “San-active” in the concentration from 1.0 to 2.0% shows very weak corrosion activity on stainless steel. The use of “San-active” detergent for the sanitary treatment of equipment surfaces in the intestinal workshop at the concentration of the working solution 1.0–2.0% and the temperature 60 ± 5 °C for 20 minutes provides 99.9–100% efficiency of sanitary treatment.

ПРАГМАТИЧНИЙ ПОТЕНЦІАЛ НАТИВНОЇ РЕКЛАМИ: ФОРМИ, ТРЕНДИ (НА ПРИКЛАДІ НАТИВНОГО КОНТЕНТУ В СОЦІАЛЬНІЙ МЕРЕЖІ FACEBOOK ЩОДО БРЕНДА ФАКУЛЬТЕТУ ЖУРНАЛІСТИКИ ЗНУ)

2020 ◽  
pp. 113
Author(s):  
V. Kovpak ◽  
N. Trotsenko
Keyword(s):  
Social Network ◽  
Professional Training ◽  
Educational Process ◽  
Communication Strategy ◽  
Student Centered ◽  
Teacher Student ◽  
The Social ◽  
National University ◽  
High Level ◽  
The Impact

<div><p><em>The article analyzes the peculiarities of the format of native advertising in the media space, its pragmatic potential (in particular, on the example of native content in the social network Facebook by the brand of the journalism department of ZNU), highlights the types and trends of native advertising. The following research methods were used to achieve the purpose of intelligence: descriptive (content content, including various examples), comparative (content presentation options) and typological (types, trends of native advertising, in particular, cross-media as an opportunity to submit content in different formats (video, audio, photos, text, infographics, etc.)), content analysis method using Internet services (using Popsters service). And the native code for analytics was the page of the journalism department of Zaporizhzhya National University on the social network Facebook. After all, the brand of the journalism department of Zaporozhye National University in 2019 celebrates its 15th anniversary. The brand vector is its value component and professional training with balanced distribution of theoretical and practical blocks (seven practices), student-centered (democratic interaction and high-level teacher-student dialogue) and integration into Ukrainian and world educational process (participation in grant programs).</em></p></div><p><em>And advertising on social networks is also a kind of native content, which does not appear in special blocks, and is organically inscribed on one page or another and unobtrusively offers, just remembering the product as if «to the word». Popsters service functionality, which evaluates an account (or linked accounts of one person) for 35 parameters, but the main three areas: reach or influence, or how many users evaluate, comment on the recording; true reach – the number of people affected; network score – an assessment of the audience’s response to the impact, or how far the network information diverges (how many share information on this page).</em></p><p><strong><em>Key words:</em></strong><em> nativeness, native advertising, branded content, special project, communication strategy.</em></p>

INNOVATIVE ARCHITECTURAL IDEAS OF THE FUTURE DWELLINGS

2020 ◽  
Vol 2 (2) ◽  
pp. 67-75
Author(s):  
Dzhyhil Yu. ◽  
Keyword(s):  
Personal Responsibility ◽  
Traffic Noise ◽  
Modern World ◽  
Future Research ◽  
Housing Design ◽  
Residential Architecture ◽  
Planning Decisions ◽  
National University ◽  
Methodological Principles ◽  
The Impact

Residential architecture has one of the most conservative styles based on its specifics. However, today this type of architecture seeks to actively respond to changes in the modern world. These changes are caused by multiple factors, among them are: technological and information progress; lack of resources and environmental pollution; military conflicts and population migration etc. The purpose of this article is to summarize the experience of the Department of Architectural Environment of Lviv Polytechnic National University over housing issues and outline the methodological principles of innovation in designing both individual houses and the architectural environment of residential formations. While writing this article, we analyzed the 15-year experience of Architectural Environment's Department diploma projects. The unique feature of these projects is the priority on the design of the environment. The evolution of diploma project topics is revealed on multiple distinctive examples such as the reconstruction of old buildings in Lviv and the construction of prefabricated homes and container-type housing. Futuristic settlements are represented in projects such as “The Ocean Settlements” and “Prykarpatsk - the City of Future”. Research projects were developed to study current trends in housing design and the impact of innovative technologies on planning decisions. These projects discoursed the protection of housing from traffic noise and issues related to environmental preservation. Innovative architectural ideas of future housing, developed by students in these projects, are based on a combination of aesthetic, technical, and social components of architecture. The conclusion indicates that when educating future architects, it is important to teach them the basics of the craft, as well as the ability to defend their innovative ideas. The emphasis is placed on the importance of educating the architect's personal responsibility for their own design solutions. It is stated that professional motivation and the ability to constantly renew previously acquired knowledge and skills will be among the main tasks in training future architects.

Factors Influencing Academic Stress on Students of University of Economics and Business - Vietnam National University

2020 ◽  
Vol 36 (3) ◽  
Author(s):  
Nham Phong Tuan ◽  
Nguyen Ngoc Quy ◽  
Nguyen Thi Thanh Huyen ◽  
Hong Tra My ◽  
Tran Nhu Phu
Keyword(s):  
Academic Stress ◽  
Stress Factors ◽  
Negative Effects ◽  
Cross Sectional ◽  
Stress Scale ◽  
Perceptions And Attitudes ◽  
National University ◽  
Questionnaire Surveys ◽  
The University ◽  
The Impact

The objective of this study is to investigate the impact of seven factors causing academic stress on students of University of Economics and Business - Vietnam National University: Lack of leisure time, Academic performance, Fear of failure, Academic overload, Finances, Competition between students, Relationships with university faculty. Based on the results of a practical survey of 185 students who are attending any courses at the University of Economics and Business - Vietnam National University, the study assesses the impact of stress factors on students. The thesis focuses on clarifying the concept of "stress" and the stress level of students, while pointing out its negative effects on students. This study includes two cross-sectional questionnaire surveys. The first survey uses a set of 16 questions to assess students’ perceptions and attitudes based on an instrument to measure academic stress - Educational Stress Scale for Adolescents (ESSA). The second survey aims to test internal consistency, the robustness of the previously established 7-factor structure. Henceforth, the model was brought back and used qualitatively, combined with Cronbach’s Alpha measurement test and EFA discovery factor analysis. This study was conducted from October 2019 to December 2019. From these practical analyzes, several proposals were made for the society, the school and the students themselves.

Impact of Raw Materials and Processing Techniques on The Microbiological Quality of Egyptian Domiati Cheese

2020 ◽  
Keyword(s):  
Raw Materials ◽  
Microbiological Quality ◽  
Raw Milk ◽  
Plate Count ◽  
E Coli ◽  
Fresh Cheese ◽  
Processing Techniques ◽  
The Impact ◽  
Total Yeast

Domiati cheese is the most popular brand of cheese ripened in brine in the Middle East in terms of consumed quantities. This study was performed to investigate the impact of the microbiological quality of the used raw materials, the applied traditional processing techniques and ripening period on the quality and safety of the produced cheese. Three hundred random composite samples were collected from three factories at Fayoum Governorate, Egypt. Collected samples represent twenty-five each of: raw milk, table salt, calf rennet, microbial rennet, water, environmental air, whey, fresh cheese, ripened cheese & swabs from: worker hands; cheese molds and utensils; tanks. All samples were examined microbiologically for Standard Plate Count (SPC), coliforms count, Staphylococcus aureus (S. aureus) count, total yeast & mould count, presence of E. coli, Salmonellae and Listeria monocytogenes (L. monocytogenes). The mean value of SPC, coliforms, S. aureus and total yeast & mould counts ranged from (79×102 CFU/m3 for air to 13×108 CFU/g for fresh cheese), (7×102 MPN/ cm2 for tank swabs to 80×106 MPN/ml for raw milk), (9×102 CFU/g for salt to 69×106 CFU/g for fresh cheese) and (2×102 CFU/cm2 for hand swabs to 60×104 CFU/g for fresh cheese), respectively. Whereas, E. coli, Salmonella and L. monocytogenes failed to be detected in all examined samples. There were significant differences in all determined microbiological parameters (p ≤0.05) between fresh and ripened cheese which may be attributed to different adverse conditions such as water activity, pH, salt content and temperature carried out to improve the quality of the product.

Antibacterial silver-diatomite nanocomposite ceramic with low silver release

2019 ◽  
Vol 20 (2) ◽  
pp. 633-643
Author(s):  
Xiaopeng Qi ◽  
Junwei Chen ◽  
Qian Li ◽  
Hui Yang ◽  
Honghui Jiang ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Water Treatment ◽  
Photoelectron Spectroscopy ◽  
Bactericidal Effect ◽  
Ceramic Filter ◽  
Ceramic Filters ◽  
X Ray ◽  
E Coli ◽  
Point Of Use ◽  
Silver Release

Abstract There is an urgent need for an effective and long-lasting ceramic filter for point-of-use water treatment. In this study, silver-diatomite nanocomposite ceramic filters were developed by an easy and effective method. The ceramic filters have a three-dimensional interconnected pore structure and porosity of 50.85%. Characterizations of the silver-diatomite nanocomposite ceramic filters were performed using scanning electron microscopy, transmission electron microscopy, X-ray diffraction, and X-ray photoelectron spectroscopy. Silver nanoparticles were confirmed to be formed in situ in the ceramic filter. The highest silver concentration in water was 0.24 μg/L and 2.1 μg/L in short- and long-term experiments, indicating very low silver-release properties of silver-diatomite nanocomposite ceramic filter. The nanocomposite ceramics show strong bactericidal activity. When contact time with Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) of 105 colony forming units (CFU)/mL exceeded 3 h, the bactericidal rates of the four different silver content ceramics against E. coli and S. aureus were all 100%. Strong bactericidal effect against E. coli with initial concentration of 109 CFU/mL were also observed in ceramic newly obtained and ceramic immersed in water for 270 days, demonstrating its high stability. The silver-diatomite nanocomposite ceramic filters could be a promising candidate for point-of-use water treatment.

scholarly journals KUALITAS AIR SUNGAI DI KAWASAN WISATA PANTAI PETITENGET, KEROBOKAN KABUPATEN BADUNG BALI

2018 ◽  
Vol 12 (2) ◽  
pp. 226
Author(s):  
Wayan Budiarsa Suyasa ◽  
Sri Kunti Pancadewi G. A ◽  
Iryanti E. Suprihatin ◽  
Dwi Adi Suastuti G. A.
Keyword(s):  
Water Quality ◽  
Structural Changes ◽  
Pollution Index ◽  
Basic Structure ◽  
Physical Damage ◽  
E Coli ◽  
Pollutant Source ◽  
Quality Category ◽  
Index Value ◽  
The Impact

In order to maintain the environmental carrying capacity of coastal tourism, this research was conducted to determine the condition of river water environmental pollution in the Petitenget beach area and pollutant source activities. Determination of water quality is carried out by analyzing the water quality taken at several sampling points in the four rivers that lead to the Petitenget beach. Determined the pollution index value (IP) of the physical chemical and biological pollution parameters. The results showed that the four rivers that flow into the Petitenget Beach area had been contaminated with indications of pH, BOD, COD, ammonia, Coliform and E. coli which exceeded water quality category III class quality (PerGub Bali No 16 Year 2016). The four rivers are included in the criteria of severe contamination. The four rivers have experienced physical damage or structural changes that have very high discharge fluctuations both in quantity and quality. Slimy basic structure, smelly and slum aesthetic waters. While the indication of the impact of pollution is waste water which is directly discharged into the river from hotels, restaurants, homestays, commercial centers and settlements.

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