scholarly journals The Antioxcidant, Antibacterial Activity and Toxicity of Kesambi (Schleichera Oleosa (Lour) Oken) Brak Extracts

2020 ◽  
Vol 4 (3) ◽  
Author(s):  
Puspita Sari
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Toxicity Test ◽  
Ethyl Acetate Extract ◽  
Hexane Extract ◽  
Diffusion Method ◽  
Toxicity Tests ◽  
Agar Diffusion ◽  
Schleichera Oleosa

Abstract This study aimed to compare antioxidant, antibacterial and toxicity tests of various types of kesambi tree bark extract (Schleichera oleosa (Lour) Oken). The research stages included extraction of kesambi stem skin from Mancak Serang sub-district with multilevel maceration techniques using 3 solvents: Methanol, n-hexane, and ethyl acetate. The extract was then tested for antioxidant activity, antibacterial, and toxicity. The results of antioxidant testing using the DPPH method, antibacterial with agar diffusion method, and toxicity test with BSLT method, the results showed that ethyl acetate extract had better antioxidant activity (IC50 7,723 ppm.) compared to extract of methanol (IC50 7,801 ppm), and n-hexane extract (IC50 8,568 ppm). Antibacterial activity showed the ability to inhibit the growth of Streptococcus aureus compared to Escherichia coli at a concentration of 10000 ppm, and the results of the toxicity test showed that ethyl acetate extract had better activity (LC50 305,17 ppm) than n-hexane extract (LC50 374, 96 ppm) and methanol extract (LC50 431,26 ppm).Keywords: DPPH, BSLT, Agar Diffusion, Schleichera oleosa (Lour) Oken

scholarly journals ANTIOXIDANT AND ANTIBACTERIAL ACTIVITY OF MANGROVE Brugueira gymnorrhiza STEM EXTRACTS AGAINST PATHOGENIC BACTERIA Vibrio cholerae

2021 ◽  
Vol 3 (2) ◽  
pp. 53-61
Author(s):  
Angga Crystal Loasana Yami ◽  
Irmanida Batubara ◽  
Kholis Abdurachim Audah
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Vibrio Cholerae ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Ic50 Value ◽  
Dpph Method

Background : The treatment of some diseases caused by free radicals and pathogenic bacteria usually by using antioxidants and antibiotics. Due to excessive use of antibiotics and other environmental cues, some bacteria are now resistant to certain antibiotics or even to multiple antibiotics. Some Vibrio cholerae bacterial strains are multiresistant to many antibiotics.Objective : The antioxidant and antibacterial activities of Brugueira gymnorrhiza stem extracts against pathogenic bacteria V. cholerae.Method : The B. gymnorrhiza stem was extracted by gradient maceration method. The DPPH method was used to determine the antioxidant activity and the disc diffusion method was used to determine the antibacterial activities. The column chromatography method was used to fractionate the selective extract with the best activity. The LC-MS/MS method was used to identify the compound obtained from the fraction with the best antioxidant and antibacterial activity.Result : Ethyl acetate extract of B. gymnorrhiza stem had the best antibacterial activity with MIC and MBC values of 62.50 mg/L. Ethyl acetate extract also showed the best value of antioxidant activity as indicated by an IC50 value of 255.03 mg/L. The results of fractions test showed that fraction 3 had the best antibacterial and the best antioxidant activities with both the MIC and MBC values of 7.90 mg/L and IC50 value of 348.91 mg/L, respectively.Conclusion : Ethyl acetate extract of B. gymnorrhiza stem has good potential as antioxidant and antibacterial. The compound which is thought as antioxidant and antibacterial from Ethyl acetate extract is 2-Ethyl-4-methyl-1H-imidazole.

scholarly journals Phytochemical analysis of Ardisia silvestris leaf extracts and their antioxidant and antibacterial activities

2020 ◽  
Vol 19 (04) ◽  
pp. 28-35
Author(s):  
Biet V. Huynh
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antibacterial Properties ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Agar Diffusion ◽  
Dpph Method ◽  
Agar Diffusion Method ◽  
Ethanol Extracts

The phytochemical analysis and antibacterial and antioxidant activity of Ardisia silvestris extracts were carried out. The bioactive compounds of Ardisia silvestris leaves were extracted with petroleum ether, ethyl acetate, ethanol and water by immersion and microwave method. The DPPH method was used to determine the antioxidant activity of Ardisia silvestris extracts. The antibacterial properties of Ardisia silvestris tested against of Staphylococcu saureus, Samonella sp., and Escherichia coli were determined by using agar diffusion method. The agar diffusion method was used to determine the antibacterial effects of both plant extracts on the test organisms. The results showed that Ardisia silvestris leaves contained compounds such as essential oils, fats, alkaloids, flavonoids, coumarins, tannins, anthocyanoids, carotenoids, organic acids, reducing agents, proanthocyanidins, saponins and anthraquinones. Ardisia silvestris leaves had a total polyphenol content of 0.26% dry matter, tannin of 8.8%, and a total flavonoid of 1.44 mg/g. The ethyl acetate extract and water extract of the leaves had the antioxidant activity and were 4.2 and 4.4 times lower than ascorbic acid, respectively. The ethyl acetate extract of Ardisia silvestris had the highest oxidative activity. The zone of inhibition of the plant extract diameters at the concentration of 100 µl/ml ranged between 9.67mm and 20.67mm for ethyl acetate and ethanol extracts, respectively on E. coli. Similarly, the zones of inhibitionof ethyl acetate and ethanol extracts diameters were 14.67 and 15.33 mm, respectively on Samonella sp., however, it was not shown for Staphylococus aureus.

scholarly journals Formulation and Evaluation of Antioxidant Peel-Off Face Mask Containing Red Dragon Fruit Rind Extract (Hylocereus polyrhizus Haw.)

2020 ◽  
Vol 20 (3) ◽  
pp. 438
Author(s):  
Trasna Arman Jani ◽  
Aliefman Hakim ◽  
Yohanes Juliantoni
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Face Mask ◽  
Hexane Extract ◽  
Product Evaluations ◽  
Drying Time ◽  
Dragon Fruit ◽  
Dispersive Power

Peel-Off face mask is one of the cosmetics that is used to treat skin from free radicals. Peel-off face mask can minimize the effects of free radicals because it contains antioxidant. One of the antioxidant sources is red dragon fruit’s rind (Hylocereuspolyrhizus Haw.). The aim of this study is to obtain Peel-Off face mask of red dragon fruit’s rind extract that contains anthocyanin. Red dragon fruit’s rind was macerated by ethanol 96%, ethyl acetate and n-hexane added citric acid (4:1) solvents. TLC test used Chloroform: ethyl acetate: n-butanol (5:4:1) eluent and sprayed by AlCl3. Antioxidant activity of extract was tested using DPPH (1,1-Diphenyl-2-Picryhidrazyl) method. Peel-Off face mask was made using PVA, HPMC, methyl paraben, propylparaben, propylenglycol, extract, ethanol 96% and aquadest. The rendemen of ethanol 96% extract was 9,476%, ethyl acetate extract was 0,783% and n-hexane extract was 0,631%. The results of TLC test showed yellow spots on the ethyl acetate and ethanol 96% extracts which indicated that extract contained flavonoids (anthocyanin). The results of antioxidant activity test showed that IC50 value of ethanol 96% extract was 189,7422 (AAI=0,2087), ethyl acetate extract was 196,9398 (AAI=0,2011), and n-hexane extract was 385,3664 (AAI=0,1027). The result of the product evaluations showed that all the formulas complied the product requirements such as the organoleptic, homogenity, pH, dispersive power and drying time. Peel-off face mask already meet the product requirements but further research is needed to test the product stability and activity.

scholarly journals KLT BIOAUTOGRAFI HASIL PARTISI EKSTRAK ETANOL HERBA BANDOTAN (Ageratum conyzoides L.) TERHADAP Shigella dysentriae

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Surya Sumantri Abdullah ◽  
Natsir Djide ◽  
Sartini Natsir
Keyword(s):  
Ethyl Acetate ◽  
Incubation Time ◽  
Ethyl Acetate Extract ◽  
Ethanol Extract ◽  
Hexane Extract ◽  
Diffusion Method ◽  
Diameter Measurement ◽  
Steroid Compound ◽  
Ageratum Conyzoides ◽  
Bioassay Method

Penelitian uji daya hambat dan analisis KLT bioautografi hasil partisi ekstrak etanol herba bandotan (Ageratum conyzoides L.) terhadap Shigella dysentriae  telah dilakukan. Penelitian ini bertujuan untuk mengetahui kemampuan ekstrak tersebut dalam menghambat pertumbuhan S.dysentriae dan membandingkan daya hambat ekstrak dengan tingkat kepolaran yang berbeda yaitu pada ekstrak n-heksana, larut etil asetat, dan tidak larut etil asetat berdasarkan  pengukurandiameter hambatan yang terbentuk. Herba tersebut diekstraksi dengan etanol menggunakan metode maserasi, lalu dipartisi dengan pelarut n-heksana dan etil asetat. Pengukuran dilakukan dengan menggunakan metode difusi pada medium Miller Hinton Agar (MHA) dengan waktu inkubasi 24 jam pada suhu 37oC dan memberikan diameter daerah hambatan terbesar pada ekstrak tidak larut etil asetat herba bandotan yaitu 9,3 dan 10,3 mm. Pemisahan secara KLT pada ekstrak n-heksana, ekstrak etil asetat, dan ekstrak tidak larut etil asetat dengan cairan pengelusi berturut-turut, n-heksana:etil asetat (3:1), n-heksana:etil asetat (1:1), dan etil asetat:etanol (10:1) dengan jumlah bercak noda berturut-turut 4, 5, dan 2. Nilai Rf pada ekstrak n-heksana 0.25, 0.41, 0.52, dan 0.71 sedangkan pada ekstrak etil asetat 0.34, 0.53, 0.65, 0.81, dan 0.92 pada ekstrak tidak larut etil asetat 0.33 dan 0.64. Hasil KLT bioautografi diperoleh komponen antibakteri yang diidentifikasi ekstrak n-heksana adalah golongan steroid dan pada tidak larut etil asetat golongan polifenol ABSTRACTInhibition test research and TLC bioautographic bioassay method of the partition results of the ethanol extract of bandotan (Ageratum conyzoides L.) herb against Shigella dysentriae have been conducted. This study aims to determine the ability of these extracts to inhibit S.dysentriae growth and to compare the inhibition of extracts with different polarity levels, n-hexane soluble, ethyl acetate soluble, and ethyl acetate insoluble extracts based on the diameter measurement of the formed resistance. The herbs were extracted with ethanol using the maceration method, then partitioned with n-hexane and ethyl acetate solvents. Measurements were carried out using the diffusion method on Miller Hinton Agar (MHA) medium with an incubation time of 24 hours at 37oC and gave the largest diameter area of resistance to the ethyl acetate insoluble extract of bandotan herb, value 9.3 and 10.3 mm. Separation by TLC on n-hexane extract, ethyl acetate extract, and ethyl acetate insoluble extract with elusive liquid respectively, n-hexane: ethyl acetate (3: 1), n-hexane: ethyl acetate (1: 1), and ethyl acetate: ethanol (10: 1) with the number of stains 4, 5, and 2, respectively. 0.81, and 0.92 in ethyl acetate insoluble extracts 0.33 and 0.64. The results of the bioautography TLC bioassay method showed that the antibacterial component identified in the n-hexane extract was a steroid compound and ethyl acetate insoluble was a polyphenol compound.

scholarly journals Study of Biological Activity and Chemical Constituent of Annona Reticulata

2016 ◽  
Vol 21 (1) ◽  
pp. 157-163 ◽  
Author(s):  
Bimala Subba ◽  
Pramod Aryal
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Column Chromatography ◽  
Authentic Sample ◽  
Chemical Constituent ◽  
Hexane Extract ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Ic50 Value ◽  
Annona Reticulata

The methanol and hexane extract of leaf of Annona reticulata, vernacularly known as ‘Sareefa’ were subjected to biological assay and column chromatography respectively. Column chromatography of the hexane extract of the leaf of A. reticulata resulted in the isolation of one pure compound PF1. The compound PF1 was suggested as β-sitosterol by comparing TLC, IR and melting point with authentic sample. The antibacterial activity against two bacteria, Staphylococcus aureus (gram positive) and Escherichia coli (gram negative) was done by agar well diffusion method and Zone of Inhibition (ZOI) was observed. The ZOI obtained ranged from 8 to 12 mm. The methanol extract of leaf showed moderate antibacterial activity on comparison with standard antibiotic streptomycin. The Antioxidant activity of the extract was tested using scavenging activity of DPPH (1, 1-Diphenyl-2- Picrylhydrazyl) radical method. The IC50 value of A. reticulata was found (41 µg/ml). The standard compound has shown IC50 value as 120 µg/ml. The antimicrobial and antioxidant activity of the extract was positively associated with the total phenolic and flavonoid contents of the extract. This study revealed that methanolic leaf extract of A. reticulata comprise effective potential source of natural antioxidant, which might be helpful in preventing the progress of various oxidative stresses.Journal of Institute of Science and TechnologyVolume 21, Issue 1, August 2016, page: 157-163

scholarly journals Penyarian Konstituen Organik Daging Buah Gaharu (Aquilaria malaccensis Lamk.) dengan Metode Maserasi Berbantu Microwave dan Uji Aktivitas Sebagai Antioksidan

KOVALEN ◽  
2021 ◽  
Vol 7 (1) ◽  
pp. 42-50
Author(s):  
Imran ◽  
Nurlian ◽  
L.A. Kadir ◽  
La Agusu ◽  
Ruslan
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Hexane Extract ◽  
Fruit Pulp ◽  
Microwave Assisted ◽  
Organic Constituents ◽  
Aquilaria Malaccensis ◽  
Ic50 Value

This study was conducted to determine the organic constituents and to test the antioxidant activity and toxicity of gaharu fruit pulp extract (Aquilaria malaccensis Lamk.). Gaharu fruit pulp extract was obtained through Microwave Assisted Extraction (MAE) method and then extracted with a solvent with different polarity, namely ethyl acetate and n-hexane. The obtained yield of ethyl acetate extract of the gaharu fruit pulp was 40.827% w/v, whereas 0% w/v in n-hexane extract, therefore, no further test for n-hexane extract, so that the n-hexane extract was not tested for the next stage. The results of the organic constituents of the ethyl acetate extract of gaharu fruit pulp showed flavonoids, saponins, glycosides, phenols and tannins. Antioxidant activity testing was carried out using the DPPH method with vitamin C as a positive control. The results of the antioxidant activity test showed that IC50 of ethyl acetate extract of gaharu fruit pulp and vitamin C were 143.789ppm and 13.797ppm, respectively. Extracts from microwave-assisted maceration that were partitioned with ethyl acetate solvent were categorized into moderate antioxidants because the IC50 value was between 101-150ppm, while vitamin C was categorized as a strong antioxidant because the IC50 value was between 0-100ppm. Toxicity testing was also carried out on ethyl acetate extract of gaharu fruit pulp using the BSLT method. The results of the toxicity test of the ethyl acetate extract of gaharu fruit pulp showed activity with an LC50 value of 11.282ppm. Based on this research, the ethyl acetate extract of gaharu fruit pulp is considered to have an anticancer potential.

scholarly journals Antibacterial Activity of Liverworts of Dumortiera hirsute (Sw.) Nees Ethyl Acetate Extract Against Pathogenic Bacteria

2021 ◽  
Vol 9 (2) ◽  
pp. 75
Author(s):  
Luthfiah Luthfiah ◽  
Dwi Setyati ◽  
Sattya Arimurti
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Antibacterial Properties ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
E Coli ◽  
Agar Well Diffusion Method

Dumortiera hirsuta is one of the liverworts that can be used as a medicinal to prevent infection by pathogenic bacteria. The content of secondary metabolites of D. hirsuta has potential as antibacterial properties includes flavonoids, alkaloids and steroids. This research is to analyze the antibacterial activity of moss D. hirsuta against pathogenic bacteria that will be beneficial to humans. Liverworts of D. hirsuta were extracted using ethyl acetate solvent and tested against three types of pathogenic bacteria using the agar well-diffusion method. The results of this study indicated that the ethyl acetate extract of D. hirsuta at 100% concentration could inhibit the growth of Escherichia coli, Staphylococcus aureus, and Salmonella typhi bacteria. The range of antibacterial activity categories of the ethyl acetate extract of D. hirsuta to E. coli, S. aureus, and S. typhi between weak to moderate.

scholarly journals In VitroAntioxidant and Cytotoxic Activities of Some Marine Sponges Collected off Misamis Oriental Coast, Philippines

2012 ◽  
Vol 9 (1) ◽  
pp. 354-358 ◽  
Author(s):  
A. P. Rivera ◽  
M. M. Uy
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Antioxidant Capacity ◽  
Ethyl Acetate Extract ◽  
Marine Sponges ◽  
Hexane Extract ◽  
Sponge Species ◽  
Cytotoxic Activities ◽  
Total Antioxidant ◽  
Unidentified Species

The phosphomolybdenum method for total antioxidant activity determination showed that the hexane, dichloromethane and ethyl acetate extracts of five marine sponge species collected off misamis oriental coast-Aaptos suberitoides, Dactylospongia elegans, Stylissa massa, Haliclonasp. and an unidentified species coded as KL-05, have varying degrees of antioxidant capacity. Expressed as ascorbic acid equivalents in μg/mL of extract, the hexane extract ofDactylospongia elegans(DeH) and the ethyl acetate extract ofAaptos suberitoides(AsE) showed the highest antioxidant capacity. Although the hexane extract of KL-05 (KL-05H) has considerable antioxidant activity, the ethyl acetate extract (KL-05E) showed no antioxidant activity. The brine shrimp assay for cytotoxicity indicated high bioactivity, withHaliclonasp.,Dactylospongia elegans, Aaptos suberitoidesandStylissa massaexhibiting high % mortality and low LC50values. The antioxidant and cytotoxic activities of the marine sponges may be attributed to the zoochemicals present. All sponge species contain alkaloids, saponins, tannins, and flavonoids. Terpenoids are present only inHaliclonasp. and the cardiac glycosides, only inAaptos suberitoidesandHaliclonasp.

scholarly journals Antioxidant Activity of n-Hexane, Ethyl Acetate and Ethanol Extract from Lakoocha Leaves (Artocarpus lacucha Buch.-Ham) using DPPH Method

2018 ◽  
Vol 1 (2) ◽  
pp. 41-47
Author(s):  
Poppy Anjelisa Zaitun Hasibuan ◽  
Mardiana
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Positive Control ◽  
Hexane Extract ◽  
Phytochemical Screening ◽  
Ic50 Value ◽  
Dpph Method

This study aimed to investigate phytochemical screening and antioxidant activity of n–hexane, ethyl acetate and ethanol extract from lakoocha leaves. The powdered simplicia was macerated with n–hexane, ethyl acetate and ethanol 96% successively, filtered, then concentrated using rotary evaporator to obtain n–hexane extract, ethyl acetate extract and ethanol extract. Phytochemical screening and antioxidant activity was performed against these extracts. Antioxidant activity was determined by DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging method using ultraviolet-visible spectrophotometer at wavelength of 516 nm after incubated for 60 minutes in dark place. Quercetin was used as positive control. The result of phytochemical screening showed n-hexane extract contains steroid, ethyl acetate extract contain steroid, tannin, glycoside, flavonoid and saponin, whereas ethanol extract contain tannin, glycoside, flavonoid and saponin. The IC50 value of n–hexane, ethyl acetate and ethanol extract was 1062.03±1.42 ppm, 323.18±0.02 ppm and 99.23±0.07 ppm respectively, whereas for quercetin was 2.32±0.01 ppm. This study showed that ethanol extract had antioxidant activity with strong category whereas n-hexane extract and ethyl acetate extract had inactive antioxidant activity with very weak categories.       Keyword: Antioxidant Activity, DPPH, Lakoocha leaf

scholarly journals UJI AKTIVITAS ANTIOKSIDAN EKSTRAK DAUN DARUJU (Acanthus ilicifolius L.) DENGAN METODE PEREDAMAN RADIKAL BEBAS 1,1-DIPHENYIL-2-PICRYLHIDRAZIL (DPPH)

2018 ◽  
Vol 5 (2) ◽  
pp. 299-308
Author(s):  
Selpida Handayani ◽  
Ahmad Najib ◽  
Nurul Purnama Wati
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Extraction Method ◽  
Leaf Extract ◽  
Ethyl Acetate Extract ◽  
Ethanol Extract ◽  
Hexane Extract ◽  
Acanthus Ilicifolius ◽  
Ic50 Value

Sea holly leaves (Acanthus ilicifolius L.) belongs to Acanthaceae family, contain flavonoid compounds, alkaloids, an phenols. This research aimed to determine the antioxidant activity of sea holly leaf extract by free radical damping method 1,1-Diphenyl-2-Picrylhydrazil (DPPH). The extraction method multilevel maseration using n-hexane extract, ethyl acetate extract, and ethanol extract is 1,55%, 0,65% and 4,97% respectively. On each extract, the antioxidant activity was assayed by DPPH free radical inhibition method by measuring is absorbance at the maximum wavelength of 515nm using UV-VIS spectrophometer with quercetin compound as comparator. The result of antioxidant assay showed that IC50 value, ethanol extract is 34,659 μg/mL (strong antioxidant), ethyl acetate extract is 162,512 μg/mL (weak antioxidant), n-hexane extract is361,730 μg/mL (not active as antioxidant).

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