scholarly journals The Effect of Green Betel Leaves (Piper betle L.) Extract on Wounding Healing in Mice (Mus musculus L.)

2021 ◽  
Vol 21 (1) ◽  
pp. 103
Author(s):  
Nurul Atika Zar’ah ◽  
Syachruddin Syachruddin ◽  
Kusmiyati Kusmiyati
Keyword(s):  
Wound Healing ◽  
Mus Musculus ◽  
Leaf Extract ◽  
Block Design ◽  
Average Diameter ◽  
Control Group ◽  
P Value ◽  
Piper Betle ◽  
Betel Leaf ◽  
The One

Green betel leaf (Piper betle L.) contains secondary metabolites, namely alkaloids, flavonoids, steroids, terpenoids, saponins, tannins. Green betel leaf (Piper betle L.) is widely used for wound healing, especially in rural Indonesians. This study aims to determine the effect of green betel leaves extract (Piper betle L.) on wound healing in mice (Mus musculus L.). The samples were determined using a randomized block design (RBD) using mice (Mus musculus L.) which consisted of three treatment groups using a concentration of 20%, 30%, 40% and one control group. The Measure of the average diameter of the wound was using a ruler on each side of the diameter. The data measurement for the average diameter of wound restriction was analyzed by using the one way. The one way Anova test showed that the sig or p-value is 0.000 <0.05 (at the alpha level of 5%) so that Ho is rejected which indicates that there is an effect of giving green betel leaf extract (Piper betel L.) on wound healing in mice (Mus musculus L.). Based on the results of the LSD or LSD test, it shows that it is not significant / the same because the sig value is 0.074> 0.05, which means that no change occurs in mice when given 30% or 40% extract. Green betel leaf extract (Piper betle L.) has an effect on wound healing in mice (Mus musculus L.).  

Efektifitas Ekstrak Daun Sirih Hijau (Piper Betle) sebagai Insektisida Nabati terhadap Mortalitas Lalat Rumah (Musca domestica)

2019 ◽  
Vol 10 (2) ◽  
pp. 44-50
Author(s):  
Rinaldi Daswito ◽  
Rima Folentia ◽  
M Yusuf MF
Keyword(s):  
Musca Domestica ◽  
Leaf Extract ◽  
Effective Concentration ◽  
P Value ◽  
Piper Betle ◽  
Microbiology Laboratory ◽  
House Flies ◽  
Betel Leaf ◽  
The One ◽  
One Way Anova

One of the diseases that can be transmitted by flies is diarrhea. Green betel leaf contains essential oils, chavicol, arecoline, phenol, and tannins which function as plant-based insecticides. This study aimed to determine the effectiveness of green betel leaf extract (Piper betel) as a plant-based insecticide on the number of mortality of house flies (Musca domestica). The research was an experimental study used After Only Design used the One Way Anova test with a 95% confidence level. The samples used were 360 ​​house flies. Each treatment of 30 house flies with 4 repetitions and used three concentrations of green betel leaf extract (25%, 50%, 75%). The study was conducted at the Chemistry and Microbiology Laboratory of Health Polytechnic Tanjungpinang, while the location of the fly collection was at the Tokojo Garbage Collection Station in Bintan Regency. The number of mortality of house flies at a concentration of 25% was 81 heads (67.5%), 50% concentrations were 93 heads (77.5%), and at a concentration of 75% were 103 heads (85.83%). There was an effect of green betel leaf extract on the mortality of house flies (p-value 0.0001 <0.05) with the most effective concentration of 75%. Further research is needed to obtain a finished product utilizing green betel leaf extract as a vegetable insecticide, especially in controlling the fly vector. Need further research on the use of green betel leaf extract as a vegetable insecticide controlling the fly vector by taking into account the amount of spraying and the age of the fly.   Keywords: Green betel leaf extract , organic insecticide, houseflies

Effects of 1% and 3% Mobe Leaf Extract Gel on Socket Wound Healing after Tooth Extraction

2021 ◽  
Vol 24 (1) ◽  
pp. 1-5
Author(s):  
Olivia Avriyanti Hanafiah ◽  
Denny Satria ◽  
Avi Syafitri
Keyword(s):  
Wound Healing ◽  
Tooth Extraction ◽  
Leaf Extract ◽  
Alveolar Bone ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
P Value ◽  
Gel Treatment

Tooth extraction is a process of removing teeth from the alveolar bone. In wound healing, fibroblast are very important cells. The main purpose of this study was to determine the effect of mobe leaf 1% and 3% extract gel (Artocarpus lakoocha) on fibroblast proliferation in post extraction tooth socket wound healing. This research used 16 samples of wistar rats, divided into 4 groups, a positive control group, a negative control group and a 1% and 3% mobe leaf extract gel group. The left mandibular incisors were extracted, then 1% and 3% gels of mobe leaf extract were applied on day 1 to day 7. Data analysis was calculated using the Kruskal-Wallis test on clinical data and one way ANOVA test for microscopic. The result of the socket wound healing activity test for a good concentration of mobe leaf extract gel was 3%. This research shows significant resultith p-value of 0.018 (< 0.05) on the closure of the socket wound clinically which means the closure of the wound accelerates because of the mobe leaf 3% extract gel treatment. The distance of fibroblast on microscopically shows significant resultith a p-value of 0.002 (< 0.05), which means that there was an enlargement of the distance fibroblast at the socket wound closure with application of mobe leaf 3% extract gel. From the results of the study it can be concluded that mobe leaf 3% extract gel has the best ability to show acceleration the closure of the socket wound either clinically or microscopically.

scholarly journals COMPARISON OF THE EFFECTIVENESS OF GIVING BINAHONG LEAF (Anredera cordifolia (Ten) Steenis) AND PAPAYA LEAF (Carica papaya) ON SKIN WOUND HEALING IN WHITE RAT (Rattus novergicus)

2021 ◽  
Vol 3 (2) ◽  
pp. 78-85
Author(s):  
Givenchy Winarjo ◽  
◽  
Fransiscus Arifin ◽  
Dave Oenarta ◽  
◽  
...  
Keyword(s):  
Wound Healing ◽  
Leaf Extract ◽  
Healing Process ◽  
Control Group ◽  
Wound Healing Process ◽  
P Value ◽  
World Population ◽  
White Rats ◽  
Significant Difference ◽  
The World

Introduction: The incidence of acute and chronic injuries increases every year. Most injuries in the world population, as many as 48%, are caused by trauma or surgery. the highest incidence of wounds in the world based on the etiology was from surgery, which was 110.30 million cases. One of the handlings of wounds on the skin can be done by giving herbal medicines, which can be easily accessed by the public, such as binahong leaves and papaya leaves. Purpose: To analyze the effectiveness of giving 40% binahong leaf extract cream and 40% papaya leaf extract cream on the wound distance in the white rat wound healing process. Method: this study is an experimental study with post-test only control group design approach. The sampling technique used in this study was a random sampling method where the number of samples was calculated using the Federer formula, with a total sample of 28 white rats. The distance of the wound was measured using a micrometer. Results: Twenty-eight wound distance on rats were measured as subjects of the study and then treated with binahong leaf extract cream and papaya leaf extract cream, then the wound distance of each treatment was measured using a micrometer. According to the results, 40% binahong leaf extract cream and 40% papaya leaf extract cream showed better effectiveness in reducing the wound distance of white rat incision than the negative control group (cream base) and positive control group (gentamicin ointment). According to the Mann Whitney test comparing the P1 group (40% binahong leaf extract cream) and P2 (40% papaya leaf extract cream), p-value >0.05, which is 0.543, which means that P1 has no significant mean difference with P2. Conclusion: There was no significant difference in effectiveness between the treatment of 40% binahong leaf extract cream and 40% papaya leaf extract cream.

scholarly journals The Influence of Bioinsecticide Granules of Srikaya (A. Squamosa) and Betel Leaves (P. Beetle) Extract Extracts on Kidney Histopathology in Mice (Mus musculus)

2020 ◽  
Vol 3 (1) ◽  
pp. 17
Author(s):  
Wike Rosalini ◽  
Sri Hernawati ◽  
Dwi Wahyuni
Keyword(s):  
Mus Musculus ◽  
Simple Random Sampling ◽  
Control Group ◽  
P Value ◽  
Safety Testing ◽  
Betel Leaf ◽  
Preliminary Examination ◽  
Post Test ◽  
Leaf P

The use of chemical insecticides in the long term and high frequency will suppress insects (mosquitoes) and vectors to be tolerant until resistant. Reports of resistance from various species of mosquitoes found in multiple countries even occur in Indonesia in Surabaya. This study aimed to analyze the effect of granules bioinsecticides on the mixture of srikaya seeds (A. squamosa) and betel leaf (P. beetle) on the histopathology of mice (M. musculus) using true-experimental (the post-test only group design). Sample taken by simple random sampling and grouped into each control group and the treatment group totaling 25 mice. Data were analyzed using simple linear regression test. The results showed an overall p-value >0.05, meaning that there was no bioinsecticide effect of a mixture of betel leaf granules (Piper beetle) and srikaya seeds (Annona squamosa) on the histopathology of the kidney of mice (Mus musculus). Required safety testing of target animals in addition to mice and a preliminary examination is needed before the research is conducted so that the results are valid Keywords: bioinsecticide; histopatology; Mus musculus

scholarly journals PENGARUH LARUTAN EKSTRAK DAUN SIRIH (Piper betle L.) 50% TERHADAP PELEPASAN ION METAL (Ni, Cr dan Fe) PADA BREKET ORTODONTIK

2017 ◽  
Vol 4 (1) ◽  
pp. 32
Author(s):  
Marzuki Akbar J. Dundu ◽  
Grahita Aditya ◽  
Eko Hadianto
Keyword(s):  
Inductively Coupled Plasma ◽  
Metal Ion ◽  
Leaf Extract ◽  
Artificial Saliva ◽  
Negative Control ◽  
Control Group ◽  
Piper Betle ◽  
Orthodontic Bracket ◽  
Betel Leaf ◽  
Significant Difference

Background: Orthodontic bracket is one of the components in a fixed orthodontic treatment. The influence of the oral cavity of an orthodontic bracket is a type IV hypersensitivity reaction due to corrosion on the bracket. The solution of extracts of betel leaf (Piper betle L.) of 50% which could affect corrosion. This study aimed to know the effect of betel leaf extract solution (piper betle L.) 50% against metal ion release.Method: The research method was “The Post Test Control Group Desain”, consists of four large groups respectively soaked with 6 units of brackets, consisting of a solution of betel leaf extract (piper betle L.) 50% (treatment), aquabidest (negative control), artificial saliva (negative control) and Naf (positive control) incubated for 7 days with a temperature of 370 C. Then ion measurement using the tool inductively coupled plasma – optical emission spectrophotometry (ICP-OES).Results: this study showed that the average release of ion the largest was on the solution of betel leaf extract (0.157167) then aquabides (0.086967), artificial saliva (0,08833) and sodium flouride (0.005533). Test results of Mann-Withney showed the presence of significant difference (p<0.05) release of ion Ni, Cr and Fe on four groups except among artificial saliva groups and sodium fluoride but the group that was soaked in a solution of betel leaf extract (piper betle L.) 50% occured a release of ion Ni, Cr and Fe higher. This is because the samples werestored in solution of betel leaf extract (Piper betle L.) of 50% which is a liquid electrolyte that can cause ion release.Conclusion: Betel leaf extract (piper betle L.) 50% is not effective to be used as an alternative to slow corrosive to metal ion (Ni, Cr and Fe) on a metal bracket.

scholarly journals Green Antibiotic Daun Sirih (Piper betle l.) Sebagai Pengganti Antibiotik Komersial untuk Penanganan Mastitis

2015 ◽  
Vol 15 (1) ◽  
pp. 28-32 ◽  
Author(s):  
Gabby Lutviandhitarani ◽  
Dian Wahyu Harjanti ◽  
Fajar Wahyono
Keyword(s):  
Block Design ◽  
Water Extract ◽  
Positive Control ◽  
Negative Control ◽  
Leaf Water ◽  
Control Group ◽  
Piper Betle ◽  
Gram Positive ◽  
Microscopic Appearance ◽  
Betel Leaf

(Green antibiotic betel leaf (Piper betle l.) as a substitute  for commercial antibiotic in mastitis treatment) ABSTRACT. The present study was carried out to investigate the possible antibacterial activity of betel leaf on the amount and microscopic appearance of mastitis-causing bacteria. The randomized block design with 5 treatment groups were : K group (mastitis milk only as negative control), Ab group (mastitis milk + antibiotic penicillin-dihydrostreptomycin as positive control),  S1 group (mastitis milk + 1,25 ml betel leaf water extract), S2 group (mastitis milk+ 2,5 ml betel leaf water extract), and S3 group (mastitis milk + 5 ml betel leaf water extract). The result showed that the amount of bacteria in the betel leaves groups (S1, S2, and S3 groups) were lower (P 0,05) than that of the control group (K group). Nonetheless the different concentration of betel leaf water extract in the S1, S2 and S3 groups did not affect to the amount of bacteria (P 0,05). Moreover, this research showed that betel leaf water extract had the same effectiveness with commercial antibiotic penicillin-dihydrostreptomycin to inhibit the growth of bacteria as indicated by the same amount of bacteria (P0,05) among the betel leaf groups (S1, S2, and S3 groups) and the Ab group. Gram-positive and negative bacteria were seen in the K group. However, only Gram-negative bacteria were visible in the betel leaf groups (S1, S2, and S3 groups) and in the Ab groups, indicating that betel leaf had the same effectiveness as penicillin-dihydrostreptomycin to inhibit the growth of Gram-positive bacteria.

PENGARUH EKSTRAK DAUN SIRIH MERAH (Piper crocatum Ruiz & Pav.) TERHADAP GLUKOSA DARAH MENCIT (Mus musculus L.) JANTAN YANG DIINDUKSI SUKROSA

BioScience ◽  
2018 ◽  
Vol 2 (1) ◽  
pp. 61
Author(s):  
Elsa Yuniarti
Keyword(s):  
Blood Glucose ◽  
Mus Musculus ◽  
Leaf Extract ◽  
Negative Control ◽  
State University ◽  
Betel Leaf ◽  
Randomized Design ◽  
The Subject ◽  
Effective Drugs ◽  
Zoology Department

Patients withdiabetes mellitus (DM) continues to grow because prosperity and people's lifestyles.Treatment of diabetes often usei njections of insulin and oral antidiabetic drugs. Thetreatment has no side effects. Therefore, it is necessary to find effective drugs using plants thatred betel leaf (Piper crocatum Ruiz & Pav.). Red betel leaf contains flavonoids which are antioxidants. This study aims to determine the effect and dose of extract of red betel leaf (Piper crocatum Ruiz & Pav.) The most effective agains blood glucose in mice(Mus musculusL.) male induced sucrose.This study was an experimental study. The research was conducted in October 2015 in the Division of Laboratory Animal and Zoology Department of Biology, State University of Padang. The subject of research in the form of mice (Mus musculus L.) males totaled 24 tails. The design used was completely randomized design (CRD) with 6 treatments and 4 repetitions. The treatment is given as follows: treatment I: the diabetes control without any treatment given, treatment II: as a negative control (sucrose 3 g/kg bw), treatment III: sucrose+suspension of red betel leaf extract (dosage 0,7 g/kg bw), treatment IV: sucrose+suspension of red betel leaf extract (dosage 1,4 g/kg bw), treatment V: sucrose+suspension of red betel leaf extract (dosage of 2,1 g/kg bw) and treatment VI: sucrose+suspension extracts red betel leaf (dosage 2,8 g/kg bw).The results showed that the extract of red betel leaf (Piper crocatum Ruiz & Pav.) at a dose of 0,8 g/kg bw 1,4 g/kg bw 2,1 g/kg bw and 2,8 g/kg bw can lowers blood glucose in mice. However, the most appropriate dose in lowering blood glucose in mice (Mus musculus L.) at 2,8 g/kg bw in mice.

PENGGUNAAN SELAPUT AMNION SEGAR PADA INSISI LUKA OPERASI SEKSIO SESAREA

2019 ◽  
Vol 1 (2) ◽  
pp. 47-55
Author(s):  
Syahredi Syaiful Adnani ◽  
Hafni Bachtiar
Keyword(s):  
Wound Healing ◽  
Caesarean Section ◽  
Amniotic Membrane ◽  
Healing Time ◽  
Cost Of Care ◽  
Control Group ◽  
P Value ◽  
Control Groups ◽  
And Control ◽  
Wound Healing Time

In the last few decades, the incidence of caesarean section is increasing in the world, especially in Indonesia. One of the way to treat tissue scar is through biologic and synthetic dressing where nowadays, amnion has been used as biologic dressing frequently. This study was conducted to determine the effect of the use of fresh amniotic membrane on wound incision Caesarean section compared with Caesarean section incision wound covered using regular gauze bandages and fixated with plaster in RS. Dr. Reksodiwiryo Padang. The design of this study is an experimen-tal study with Post test design with control group design. Sampling was done using a formula consecutive sampling two different test samples obtained an average of 72 people for each group. The analysis used include univariate and bivariate analyzes. The average wound healing time the difference was statistically significant (p value <0.05) in the treatment and control groups. There was highly significant difference in the proportion of local infection on day 3 between the treatment and control groups (p value <0.05). There were very significant differences in the proportion of local allergic reactions at day 3, and 5 between the treatment and control group (p <0.05). There are significant differences in terms of the cost of care per day between treatment and control groups (p <0.05). From this study, the average wound healing time has a very significant difference.Keywords: Fresh Amniotic Membrane, Wound Cesarean Section, Wound Healing

Potential Antiseptic of Rhodomyrtus tomentosa leaves extract on Healing wound in Male Wistar rats

2021 ◽  
pp. 3143-3149
Author(s):  
Endang Sri Purwanti Ningsih ◽  
Noorlaila Noorlaila ◽  
Ikhwan Rizki Muhammad ◽  
Windy Yuliana Budianto
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Wound Care ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Rhodomyrtus Tomentosa

Background: The process of wound healing is influenced by various factors such as age, hormones, and wound care. Wound care is done to accelerate wound healing which can be done by various methods, one of them is traditional care. Traditional wound care can use medicinal plants. Rhodomyrtus tomentosa is a medicinal plant that has an antioxidant, anti-inflammatory, antitumor and antibacterial content. Thus this study aims to evaluate the effectiveness of the antiseptic solution of the Rodhomyrtus tomentosa leaf extract on wound healing in male Wistar rats. Method: this research is pure experimental research with post test only control group design. Thirty male white rats were divided into five groups, namely negative control, positive control, Rhodomyrtus tomentosa leaf extract 15%, 30%, and 60%. Rhodomyrtus tomentosa leaf extraction was carried out by maceration method with 70% ethano solvent. The extraction results are divided into 3 concentrations (15%, 30% and 60%). The wound healing process was evaluated by measuring the length of the wound manually from 0 to 10 days in each group. Meanwhile, the number of fibroblast cells was calculated through hematoxylin eosin (HE) staining and observed using an Olympus CX41 microscope with a 10x magnification and objective lens magnification in 3 fields. Result: There was a significant difference in the reduction in wound length (p =< 0,000) between the five experimental groups (Rhodomyrtus tomentosa leaf extract solution 15%, 30% and 60%, negative control and positive control. Solution of rhodomyrtus tomentosa leaf extract accelerated the increase in the number of fibroblasts compared to the negative control group (p = 0.003), but did not make a difference (p = 0.403) with the positive control group. Rhodomyrtus tomentosa leaf extraction solution had the same microscopic effect on the number of fibroblasts with a positive control group given 0.9% NaCl solution. Conclusion: There was a significant difference in the number of fibroblasts between all groups, but no difference in wound healing length.

scholarly journals In vivo antidiarrheal study of ethanolic extracts of Mikania cordata and Litsea monopetala leaves

2015 ◽  
Vol 10 (3) ◽  
pp. 562 ◽  
Author(s):  
Fatema Nasrin ◽  
Md. Lukman Hakim
Keyword(s):  
Body Weight ◽  
Castor Oil ◽  
Leaf Extract ◽  
Positive Control ◽  
Control Group ◽  
P Value ◽  
Leaf Extracts ◽  
Ethanolic Extracts ◽  
Antidiarrheal Activity

<p class="Abstract">In this study the antidiarrheal activity of ethanolic extracts of the leaves of <em>Mikania cordata</em> and <em>Litsea monopetala</em> was evaluated. Diarrhea was induced in mice by oral administration of castor oil (0.5 mL) 30 min after the administration of the extracts. During a 4 hour study the number of diarrheal feces and percentage inhibition of the extracts (200 and 400 mg/kg body weight) was determined. Loperamide (3 mg/kg body weight) served as standard and belonged to the positive control group. The extracts exhibited potent antidiarrheal activity as well as achieved statistically significant p value (p&lt;0.01 and p&lt;0.05) compared to control group. Among the extracts the highest percentage inhibition of defecation (60%) was recorded for leaf extract (400 mg/kg body weight) of <em>L. monopetala</em>. So, the study corroborates the significant antidiarrheal activity of <em>M. cordata</em> and <em>L. monopetala</em> leaf extracts and raises the demand of further sophisticated investigation.</p><p> </p>

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