scholarly journals Evaluation of Pyloroplasty repair by Single layer Simple Interrupted Extra Mucosal technique in Dogs

2017 ◽  
Vol 41 (1) ◽  
pp. 38-42
Author(s):  
Humam H. Nazht
Keyword(s):  
Histopathological Examination ◽  
Physiological Activity ◽  
Healing Process ◽  
Radiological Finding ◽  
Single Layer ◽  
Inflammatory Cells ◽  
Pyloric Region ◽  
Post Operation ◽  
Good Patency ◽  
Clinical Changes

    In this study a pyloroplasty technique was designed by using a single layer simple interrupted extra-mucosal suture pattern on pyloric region in dogs. Nine adult dogs were used to induce longitudinal surgical incision in the pyloric region under general anesthesia using a combination of 15mg/kg B.W. ketamine hydrochloride 5% and 5 mg/kg B.W Xylazine hydrochloride 2%. The incision was closed by pyloroplasty technique (longitudinal incision for whole layers with transverse suturing) using single layer simple interrupted extra mucosal technique. The examination achieved by daily clinical observation, radiological finding, at the end of 21st day post operation, macroscopical and histopathological examination at the period 3rd, 7th, and 21st days post operation. The results showed no significant clinical changes in body temperature and physiological activity. The radiological findings showed good patency of the pyloric canal after 21st days post operation. The macroscopic finding shows heavy amount of the omentum adhered around the site of incision. The microscopic finding showed good healing process by re-epithelization of the mucosal layer associated with granulation tissue proliferation, angiogenesis, and less infiltration of mononuclear inflammatory cells. The conclusion indicated that single layer simple interrupted extra mucosal suture pattern technique can be used successfully in repair the pyloric stenosis in dog.

scholarly journals In vivo evaluation of a recombinant N-acylhomoserine lactonase formulated in a hydrogel using a murine model infected with MDR Pseudomonas aeruginosa clinical isolate, CCASUP2

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Masarra M. Sakr ◽  
Walid F. Elkhatib ◽  
Khaled M. Aboshanab ◽  
Eman M. Mantawy ◽  
Mahmoud A. Yassien ◽  
...  
Keyword(s):  
Survival Rate ◽  
Murine Model ◽  
Histopathological Examination ◽  
Healing Process ◽  
Bacterial Count ◽  
Treated Group ◽  
Control Group ◽  
In Vivo Evaluation ◽  
Systemic Spread

AbstractFailure in the treatment of P. aeruginosa, due to its broad spectrum of resistance, has been associated with increased patient mortality. One alternative approach for infection control is quorum quenching which was found to decrease virulence of such pathogen. In this study, the efficiency of a recombinant Ahl-1 lactonase formulated as a hydrogel was investigated to control the infection of multidrug resistant (MDR) P. aeruginosa infected burn using a murine model. The recombinant N-acylhomoserine lactonase (Ahl-1) was formulated as a hydrogel. To test its ability to control the infection of MDR P. aeruginosa, a thermal injury model was used. Survival rate, and systemic spread of the infection were evaluated. Histopathological examination of the animal dorsal skin was also done for monitoring the healing and cellular changes at the site of infection. Survival rate in the treated group was 100% relative to 40% in the control group. A decrease of up to 3 logs of bacterial count in the blood samples of the treated animals relative to the control group and a decrease of up to 4 logs and 2.3 logs of bacteria in lung and liver samples, respectively were observed. Histopathological examination revealed more enhanced healing process in the treated group. Accordingly, by promoting healing of infected MDR P. aeruginosa burn and by reducing systemic spread of the infection as well as decreasing mortality rate, Ahl-1 hydrogel application is a promising strategy that can be used to combat and control P. aeruginosa burn infections.

Molecular Detection and Phylogenetic Analysis of Chicken Astrovirus Associated with Poultry Enteritis

2021 ◽  
Author(s):  
C. Patidar ◽  
D.K. Sharma ◽  
R. Singathia ◽  
P. Suthar ◽  
A. Saraswat ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Characterization ◽  
Histopathological Examination ◽  
Villous Atrophy ◽  
Inflammatory Cells ◽  
Liver Parenchyma ◽  
Amino Acid Sequences ◽  
Poultry Birds ◽  
Commercial Poultry ◽  
Liver Specimen

Background: Poultry enteritis is an important multifactorial disease. Chicken Astrovirus (CAstV) usually associated with enteritis. The aim of this study was to investigate the occurrence of CAstV in poultry enteritis cases, its molecular characterization, phylogenetic analysis and gross and microscopic examination of intestine and liver specimen affected with CAstV. Methods: Total 604 dead poultry birds from commercial poultry farms affected with enteritis were examined for presence of CAstV. Intestinal samples of four birds were pooled to make one biological sample. CAstV was detected by reverse transcriptase PCR (RT-PCR) using ORF-1b gene specific primers. Molecular characterization was carried out by partial gene sequencing. Result: CAstV was detected in 20.52% (31/151) of samples. Highest prevalence (49.29%) was observed in 0-1 week old chicks. The partial molecular characterization revealed high similarity of the nucleotide sequence from India (97% to 93%) and from USA, Brazil, Poland and Korea (94 to 92%). Further similarity of amino acid sequences of CAstV from India (100% to 98%) and from USA, Brazil, Poland and Korea (98 to 97%) was observed. Histopathological examination revealed villous atrophy, congestion and atrophic cystic glands in sub-mucosa of intestine. Further severe congestion and hemorrhages along with infiltration of inflammatory cells in liver parenchyma was observed.

The ethanol leaf extract of Andrographis paniculata blunts acute renal failure in cisplatin-induced injury in rats through inhibition of Kim-1 and upregulation of Nrf2 pathway

2018 ◽  
Vol 30 (2) ◽  
pp. 205-217 ◽  
Author(s):  
Bisi O. Adeoye ◽  
Ademola A. Oyagbemi ◽  
Ebunoluwa R. Asenuga ◽  
Temidayo O. Omobowale ◽  
Adeolu A. Adedapo
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Serum Creatinine ◽  
Leaf Extract ◽  
Histopathological Examination ◽  
Hematological Parameters ◽  
Kidney Tissue ◽  
Inflammatory Cells ◽  
Andrographis Paniculata ◽  
Markers Of Oxidative Stress

Abstract Background Cisplatin (CP) is a novel drug of choice in the treatment of cancer but its major limitation is nephrotoxicity, which is dose limiting. Andrographis paniculata (AP) is a common Indian dietary component. It is well known for its medicinal properties. This present study investigated the nephroprotective effect of ethanol leaf extract of Andrographis paniculata (EEAP) on CP-induced nephrotoxicity. Methods CP was used to induce nephrotoxicity in male Wistar rats to study the effect of EEAP on renal damages using hematological parameters, biochemical parameters, histology, and immunohistochemistry studies. Results The effects of EEAP were determined by CP-induced changes in different kidney tissue on antioxidant enzymes, markers of oxidative stress, serum creatinine, and urine parameters. Administration of EEAP (200 mL/kg and 400 mg/kg orally), prior to and following a single dose CP treatment (10 mg/kg i.p), significantly mitigated the CP-induced decrease in antioxidant enzymes, and increase in markers of oxidative stress, serum creatinine, and urinary protein. On histopathological examination of the kidney tissue, there was severe glomerular degeneration and infiltration of inflammatory cells in CP only treated rats, mild glomerular degeneration, and infiltration of inflammatory cells in EEAP pre-treated rats. Furthermore, EEAP activated Nrf2 and mitigated Kim-1 pathways in CP-induced nephrotoxicity. Conclusions The results showed the protective effect of EEAP against CP-induced nephrotoxicity.

scholarly journals Evaluation of acute and subacute toxicity of ethanolic extract and fraction of alkaloids from bark of Aspidosperma nitidum in mice

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Heliton Patrick Cordovil Brígido ◽  
Everton Luiz Pompeu Varela ◽  
Antônio Rafael Quadros Gomes ◽  
Mirian Letícia Carmo Bastos ◽  
Andre de Oliveira Feitosa ◽  
...  
Keyword(s):  
Biochemical Parameters ◽  
Histopathological Examination ◽  
Ethanolic Extract ◽  
Control Groups ◽  
Clinical Evaluations ◽  
Subacute Toxicity ◽  
Alkaloid Fraction ◽  
Hematological And Biochemical Parameters ◽  
Clinical Changes ◽  
Negative Controls

AbstractThis study investigated the acute and subacute toxicity of the ethanolic extract (EE) and alkaloid fraction (FA) from A. nitidum. The EE was obtained from trunk bark with ethanol, FA was obtained from the fractionation of EE. To test the acute toxicity, mice were divided into four groups, and the negative controls received water or aqueous solution of dimethyl sulfoxide, whereas the others received EE or FA (2000 mg/kg, orally, single dose). The same controls were used in the subacute trial. However, the animals were treated for 28 days, and the dose used was 1000 mg/kg per day of EE and FA. Daily clinical evaluations of the animals were performed. At the end of the experiment, hematological, biochemical, and histopathological assessments (liver, lung, heart, and kidney) were performed. In the acute and subacute toxicity studies, mice treated with EE and FA did not show any clinical changes, there were no changes in weight gain, hematological and biochemical parameters compared to the control groups (p > 0.05). In the histopathological examination, there was no abnormality in the organs of the treated animals. Therefore, EE and FA did not produce toxic effects in mice after acute and subacute treatment.

scholarly journals Evaluation of effect of local exogenous application of Myrrh oil on healing of wound incisions of facial skin (Histochemical, Histological and Histomorphometrical study in rabbits)

2019 ◽  
Vol 31 (4) ◽  
Author(s):  
Nawar Bahjet Kamil ◽  
Nada M. Al-Ghaban
Keyword(s):  
Healing Process ◽  
Inflammatory Cells ◽  
Control Group ◽  
Facial Skin ◽  
Histological Findings ◽  
Control Groups ◽  
Mean Values ◽  
Significant Difference ◽  
The Right ◽  
New Zealand Rabbits

Aim of the study: Is to evaluate the effect of myrrh oil local application on the healing process of skin wounds histologically , histomorphometrically and , histochemically. Materials and methods:Twenty male white New Zealand rabbits were used in this study. An incisional wounds with full thickness depth and of 2 cm length were done on both sides of the cheek skin of each rabbit. The left sided incisions (the control group) were irrigated with distilled water (10µL). The right sided incisions (the experimental groups) were treated with myrrh oil (10µL). Each group was subdivided into 4 subgroups according to the healing interval into 1,3,7 and 14 days(5 rabbits for each group). Results: Histological findings of our current study showed a highly significant difference between the experimental and the control groups in count of the inflammatory cells which showed that mean values increased with time for the control and the experimental groups. The histomorphometrical findings had shown that the thickness of the epithelium was nearly completed at about 7 days for the experimental groups and at about 14 days for the control. The blood vessels count was recorded to have a highly significant difference between the groups at days 1 and 3 only. The histochemical findings had shown that the collagen fibers remodeling had recorded a highly significant difference between the control and the experimental groups at days 7 and 14. Conclusion: The current study had revealed that myrrh oil accelerates wounds healing in rabbits. Key word:Wound healing , Myrrh oil, Masson’s Trichrome staining.

scholarly journals EFEKTIFITAS ANTARA PERAWATAN LUKA DENGAN MENGGUNAKAN NaCl 0,9% DAN BETADIN TERHADAP PROSES PENYEMBUHAN LUKA POST OPERASI

2020 ◽  
Vol 7 (1) ◽  
pp. 782-788
Author(s):  
Sri Lestari ◽  
Kunidah Kunidah
Keyword(s):  
Wound Healing ◽  
Wound Care ◽  
Healing Process ◽  
Care Patient ◽  
Sectio Caesarea ◽  
Wound Healing Process ◽  
P Value ◽  
Purposive Sampling ◽  
Healing Wound ◽  
Post Operation

Penyembuhan luka merupakan suatu proses yang kompleks karena berbagai kegiatan bio-seluler, bio-kimia terjadi berkesinambungan. Sifat penyembuhan pada semua luka sama, dengan variasinya bergantung pada lokasi, keparahan, dan luasnya cedera. Tujuan penelitian ini untuk mengetahui efektifitas antara perawatan luka menggunakan NaCl 0,9% dengan betadin terhadap proses penyembuhan luka post operasi pada pasien sectio caesarea. Penelitian ini merupakan jenis penelitian Quaisy Experimen. Dengan populasi seluruh pasien perawatan luka post operasi sectio caesarea yaitu 26, dan menggunakan teknik sampel purposive sampling dengan jumlah 13 responden setiap kelompok. Penelitian ini dilakukan di Rumah Sakit Ciremai Kota Cirebon. Hasil penelitian ini adalah perawatan luka post operasi sectio caesarea menggunakan NaCl 0,9% sebanyak 13 responden dengan proses penyembuhan luka ≤ 3 hari di dapatkan rata-rata 7,07. Dan hasil bivariate menunjukkan bahwa ada perbedaan antara efektifitas proses penyembuhan luka pada perawatan luka pasien post operasi sectio caesarea menggunakan NaCl 0,9% dengan nilai p value = 0,000 < (a=0,05).Kata kunci     : Proses penyembuhan luka, perawatan luka, NaCl 0,9%, Betadin ABSTRACTWound healing is a complex process because of the various activies of bio-chemical, bio-cell occurs continuosly. Wound healing properties on the all the same, with variations depending on the location, saverity, and the extent of the injury. The purpose of this reseach was to determine the effectiveness of wound care using NaCl 0,9 % with using betadin to wound healing process post operation on patients sectio caesarea. This research is a kind of the quaisy experimental. With a population of whole patients wound care post operatition sectio caesarea which is 26, and using the tecnique of purposive sampling with a total of 13 respondents per-group. The research was conducted at the ciremai hospital of cirebon city.The results of this research are wound care post operation sectio caesarea using NaCl 0,9% as much 13 respondents with the healing process of wound ≤ 3 days obtained of average 7,07. Bivariate and result showed the there is between the effectiveness of the process of wound healing in wound care patient post operation sectio caesarea using NaCl 0,9% with a value p = 0,000 < (a=0,05).Keywords      :     The process of wound healing, wound care, NaCl 0,9%, betadin

scholarly journals 16. Healing Process Of Burns (Vulnus combustion) Degrees IIB Using Mixed Leaf (Spondias dulcis F.) Fresh And Dry With Vaselin In Rats (Rattus Norvegicus)

2019 ◽  
Vol 13 (1) ◽  
pp. 114-124
Author(s):  
Ummu Balqis ◽  
Mirna Safrani Fauzi ◽  
Zuhrawati NA ◽  
Nazaruddin Nazaruddin ◽  
Razali Daud ◽  
...  
Keyword(s):  
Rattus Norvegicus ◽  
Inflammatory Cell ◽  
Healing Process ◽  
Inflammatory Cells ◽  
Inflammatory Cell Infiltration ◽  
Cell Infiltration ◽  
Treatment Groups ◽  
White Rats ◽  
Grade Ii ◽  
Hematoxylin And Eosin

           The aims of this research was looked to determine the healing process of burns  Grade II B using a mixture of fresh and dried leaf kedondong with vaseline white rats (Rattus norvegicus). Animals used were 18 rats. This study was designed using three treatment groups namely (KI) vaseline, (KII) mixture of crushed leaves and fresh kedondong (KIII) mixture of crushed dried leaves kedondong and each two replications. Burns made on the backs of mice and the treatments are done twice a day for 21 days.Observation research parameter is the description of macroscopic and microscopic observed at day 7, 14, and 21 in the skin tissue with hematoxylin and eosin staining. The observation of macroscopic studies showed that the formation of a reddish color, presence of edema, and loss of the fastest consecutive scab is KI, KIII, and KII, while the formation of a scab fastest respectively KIII, KI and KII. The observation of histopathology showed on day 7, KI, KII and KIII found inflammatory cell infiltration, hemorrhage and edema with the spread of many.on the 14th day of KI and KII infiltration of inflammatory cells and their udema much, whereas KIII inflammatory cell infiltration decreased, and hyperemia increase, on the 21st day KI, KII and KII inflammatory cell infiltration, hyperemia and hemorrhage spread with a little, but KI and KII oedemanya still being spread. Based on the results of this study concluded that KIII accelerate the healing process of burns on rats

scholarly journals Influence of nicotine on healing of vesical sutures in rabbits

2007 ◽  
Vol 22 (6) ◽  
pp. 479-484 ◽  
Author(s):  
Christiano Machado ◽  
Eduardo Wei Kin Chin ◽  
Sérgio Ossamu Ioshii ◽  
Renato Tâmbara Filho ◽  
Aline Von Bathen
Keyword(s):  
Tensile Strength ◽  
Statistical Difference ◽  
Saline Solution ◽  
Healing Process ◽  
Bladder Wall ◽  
Inflammatory Cells ◽  
Strength Test ◽  
Control Group ◽  
Time Intervals ◽  
Tensile Strength Test

PURPOSE: To study the effects of nicotine on inflammatory cells, deposition of collagen and its interference on the strength of tissue in vesical sutures in rabbits. METHODS: Twenty New Zealand rabbits were used, randomized into two groups: group N, consisting of 10 animals, to which nicotine was administered in the dose of 2mg/kg of weight as a subcutaneous injection, diluted in 1ml of saline solution at 0.9% in a daily administration during the 28 days prior to the surgery; and group C, consisting of 10 animals, to which saline solution at 0.9% was administered in the same conditions and time intervals of the nicotine group. All the animals underwent cystotomy and suture of the bladder wall 28 days after the administration of nicotine or saline solution. The measurements were performed on the fourth and seventh day in each group after cystectomy and euthanasia of the animals. A fragment of bladder was removed and sent for a tensile strength test to evaluate the tissue strength and another fragment underwent a histological analysis of inflammatory process and deposition of collagen. RESULTS: There was a decrease of neutrophils on the fourth postoperative day (p=0.079) and an increase of plasmocytes on the seventh postoperative day (p=0.053) in the animals that were given nicotine, without statistical difference in relation to the control group. In the analysis of the proliferation of fibroblasts, deposition of collagen and tensile strength test, there was no statistical difference in relation to the control group. CONCLUSION: The administration of nicotine in rabbits did not influence the healing process of vesical suture in relation to the inflammatory cells, deposit of collagen and tissue strength of the suture.

A Comparison of the Healing Process of Two Single-Layer Suture Techniques for Experimental Small Bowel Anastomosis

1992 ◽  
Vol 9 (4) ◽  
pp. 212-216 ◽  
Author(s):  
J. Waninger ◽  
G. Nöldge ◽  
L.A. Shah ◽  
M. Gorenflo
Keyword(s):  
Small Bowel ◽  
Healing Process ◽  
Single Layer ◽  
Suture Techniques ◽  
Bowel Anastomosis

scholarly journals Lung Histopathology of Laying Hens Infected by Colibacillosis in the Animal Cages Experiments of the Disease Investigation Center 6, Denpasar, Bali

2019 ◽  
Vol 3 (2) ◽  
pp. 25
Author(s):  
Kadek Ayu Trisna Yanti ◽  
Iriani Setyawati ◽  
Ni Putu Adriani Astiti
Keyword(s):  
Laying Hens ◽  
Histopathological Examination ◽  
Inflammatory Cells ◽  
Gallus Gallus Domesticus ◽  
Directorate General ◽  
Lung Histopathology ◽  
Live Stock ◽  
Hematoxylin Eosin ◽  
Paraffin Method ◽  
Neutral Buffer Formalin

This study aimed to determine the lungs histopathology of laying hens (Gallus gallus domesticus) at the Animal Cage Experiments in the Disease Investigation Center 6, Directorate General of Live Stock (DIC-6 DGLS), Denpasar, Bali, which died from colibacillosis infection. Sample of lungs were cut transversely then put into 10% of Neutral Buffer Formalin, then processed histologically by paraffin method and stained with Hematoxylin-Eosin. Observation under microscope (magnification 100x and 400x) was done for histopathological examination. Laying hens died from colibacillosis infection showed that their lungs were infected by colibacillosis, and there were found 62.50% of necrosis, 75% of inflammatory cells infiltration and 80% of hemorrhage in the lungs.

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