Screening of wound healing effect of Elaeis guineensis Oil, extract of Vernonia amygdalina mixed with dried egg albumin on burn wound inflicted guinea pig

Combined action of Vernonia amygdalinaone of the herbs belonging to the family Asteraceae and other pharmacognostic raw materials has been used in traditional medicine for ages. It produces quick therapeutic results in the treatment of various conditions and diseases such as malaria, diabetes, inflammation, hypertension to mention but few. Aim: This work is the“screening of wound healing effect of Elaeis guineensis oil, extract of vernonia amygdalina, mixed with egg albumin on burn wound inflicted guinea pig”. Method: Five male guinea pigs were used for the study. Burn injury was inflicted on the anaesthetized guinea pigs using hot metallic knife. The extract at different doses (low, medium and high conc.) was applied on the injury for subject A – C respectively, subject D (Negative control) was cleaned with only Normal saline and Dermazin cream (Positive control) was applied on the injury for subject E. Result: The measurement of wound contraction and epithelialization were observed and results were recorded after 7days, 14days and 21days respectively. There was initial increase in burn wound surface area in the guinea pig used as negative control before the wound contraction and epithelilization reduced a bit. There was rapid reduction in the wound size of the guinea pig treated with the medium concentration of the extract and the dermazin cream (positive control), although the dermazin healed neatly, the medium concentration of the extract healed faster. The results obtained encourage the use of the mixture of v. amygdalina, palmoil and egg albumin in wound healing.

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PENGARUH PEMBERIAN EKSTRAK PROPOLIS LEBAH TANPA SENGAT PADA PENYEMBUHAN LUKA BAKAR TIKUS PUTIH (Rattus norvegicus)

Jurnal Bioteknologi & Biosains Indonesia (JBBI) ◽

10.29122/jbbi.v8i1.4585 ◽

2021 ◽

Vol 8 (1) ◽

pp. 68-76

Author(s):

Nelky Suriawanto ◽

Evi Setyawati ◽

Narwan

Keyword(s):

Wound Healing ◽

Rattus Norvegicus ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Stingless Bee ◽

Control Group ◽

Burn Wound ◽

Propolis Extract ◽

Burn Wound Healing

The Effect of Treatment Using Stingless Bee Propolis Extract on Burn Wound Healing in Rattus norvegicus Burn would occurs as a result of direct or indirect fire burns, exposure to sunlight and chemicals. This study aims to analyze the effect of stingless bee propolis extract (Tetragonula fuscobalteata) on the burn wound healing of the back skin of Rattus norvegicus. An experimental research was carried out using the control and experimental groups. Twenty five R. norvegicus were divided into 5 groups: the negative control group was given 70% ethanol, the positive control group was given Bioplacenton® and 3 groups were treated with propolis extract with a concentration of 50%, 70% and 100%. Measurement of burn diameter was carried out every 2 days for 21 days. Data were analyzed statistically using the one way Anova method. The results of phytochemical screening showed that the stingless bee propolis extract contained flavonoids, phenolics, tannins and saponins. The test of the effectiveness of propolis extract against burn wound obtained a sig value of 0.00 (< 0.05). stingless bee propolis extract could heal burn wound in R. norvegicus. Luka bakar dapat terjadi akibat terbakar api secara langsung atau tidak langsung, paparan sinar matahari dan bahan kimia. Penelitian ini bertujuan untuk menganalisis pengaruh ekstrak propolis lebah tanpa sengat (Tetragonula fuscobalteata) terhadap penyembuhan luka bakar pada kulit punggung Rattus norvegicus. Metode yang digunakan merupakan penelitian true experimental menggunakan kelompok kontrol dan eksperimen. Dua puluh lima ekor R. norvegicus dibagi menjadi 5 kelompok: kelompok kontrol negatif diberi etanol 70%, kontrol positif diberi Bioplacenton®, dan 3 kelompok perlakuan ekstrak propolis dengan konsentrasi 50%, 70%, dan 100%. Pengukuran diameter luka bakar dilakukan setiap 2 hari selama 21 hari. Data dianalisa secara statistik menggunakan metode Anova satu arah. Hasil skrining fitokimia ekstrak propolis lebah tanpa sengat positif mengandung senyawa flavonoid, fenolik, tanin, dan saponin. Uji efektivitas ekstrak propolis terhadap luka bakar didapatkan nilai sig 0,00 (< 0,05). Ekstrak propolis lebah tanpa sengat dapat menyembuhkan luka bakar pada R. norvegicus.

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MANFAAT EKSTRAK ETANOL DAUN REMEK DAGING (Hemigraphis colorata W. Bull) TERHADAP LUKA BAKAR PADA TIKUS

Jurnal Tumbuhan Obat Indonesia ◽

10.22435/jtoi.v13i1.2823 ◽

2020 ◽

Vol 13 (1) ◽

pp. 32-41

Author(s):

Lusi Putri Dwita ◽

Vera Ladeska ◽

Aisyah Ramadhani ◽

Dwi Rahma Augusta ◽

Retno Tri Saufia

Keyword(s):

Wound Healing ◽

Topical Application ◽

Ethanolic Extract ◽

Positive Control ◽

Negative Control ◽

Silver Sulfadiazine ◽

Burn Wound ◽

Burn Wounds ◽

Wound Induction ◽

Collagen Density

ABSTRACT Remek daging (Hemigraphis colorata W.Bull) have been studied and used traditionally for wound healing. This study aimed to determine the effect of topical application of remek daging leaves ethanolic extract 70% on the burn wound. The animals used for this study were 30 rats, divided into five groups, namely 20, 10, 5% remek daging extract ointment, negative control (vaseline flavum), and positive control (silver sulfadiazine 1%). Histology observations were held on days 3, 7, and 14 after burn wound induction. Histological observations showed an increase number of macrophages, fibroblasts, collagen density, and re-epithelialization in the extract ointment group significantly compare to the negative control (p <0.05). The application of ointment extract 20% to the rats showed comparable results to silver sulfadiazine 1% (p> 0.05). It can be concluded that remek daging ointment extract can accelerate the healing of burn wounds with the best results at a concentration of 20%. Keywords: Hemigraphis colorata, burns, macrophages, fibroblasts, collagen. ABSTRAK Remek daging (Hemigraphis colorata W.Bull) telah diteliti dan digunakan untuk penyembuhan luka secara tradisional. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak etanol 70% daun remek daging secara topikal pada luka bakar tikus putih. Hewan yang digunakan adalah 30 ekor tikus yang dibagi menjadi 5 kelompok yaitu kelompok salep ekstrak daun remek daging 20, 10, 5% (%b/b), kontrol negatif (vaselin flavum) dan kontrol positif (silver sulfadiazine 1% (%b/b)). Pengamatan secara histologi dilakukan pada hari ke 3, 7 dan 14 setelah induksi luka bakar. Pengamatan histologi menunjukkan peningkatan jumlah makrofag, jumlah fibroblas, kepadatan kolagen dan ketebalan re-epitelisasi pada kelompok salep ekstrak daun remek daging secara signifikan dibandingkan kontrol negatif (p<0,05). Tikus yang diberikan perlakuan salep ekstrak 20% menunjukkan hasil sebanding dengan silver sulfadiazin 1% (p>0,05). Dapat disimpulkan bahwa salep ekstrak daun remek daging dapat mempercepat penyembuhan luka bakar dengan hasil terbaik pada konsentrasi 20%. Kata kunci: Hemigraphis colorata, luka bakar, makrofag, fibroblas, kolagen.

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PENGARUH INFUSA DAUN KEMBANG BULAN (Tithonia difersifolia) PADA KECEPATAN PENYEMBUHAN LUKA BAKAR

Jurnal IPTEKS Terapan ◽

10.22216/jit.2018.v12i3.583 ◽

2018 ◽

Vol 12 (3) ◽

pp. 230

Author(s):

Dwi Mulyani

Keyword(s):

Wound Healing ◽

Positive Control ◽

Negative Control ◽

Full Thickness ◽

Sprague Dawley ◽

Burn Wound ◽

Rat Models ◽

Sprague Dawley Rats ◽

Healing Wound ◽

Heated Metal

The aim of this investigation was a clinical evaluation of the accelerating effect of Tithonia difersifolia extract on the burn <a title="Find more articles at http://www.scialert.net/asci/result.php?searchin=Keywords&cat=&ascicat=ALL&Submit=Search&keyword=wound+healing (wound healing)" href="http://www.scialert.net/asci/result.php?searchin=Keywords&cat=&ascicat=ALL&Submit=Search&keyword=wound+healing" target="_blank">wound healing</a> in male Sprague-Dawley rats weighted 200-350. The sample then extracted using wet rendering (infusa) method using aqua-dest as extractor. The subjects were nine male Sprague-Dawley rats devided in to three group (negative control, positive control and experimental). Every group consist of three rats. Heated metal plat (d=2cm) used to get full thickness burn wound on dorsal rat part. Then, Tithonia difersifolia folium extract was given to wound healing(plannimetri method) for 14 days, negative control(aqua dest) 26 days and positive control (Bioplasenton) 20 days. The results with analysis Anova showed that Tithonia difersifolia folium extract have significant effect (p < 0,05) to wound healing of rat models.

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The Superior Outcome of Collagen Nanosilver in Increasing Macrophage and Collagen on Deep Dermal Burn Wound Healing

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2022.7779 ◽

2022 ◽

Vol 10 (B) ◽

pp. 31-35

Author(s):

Anggun Ari Mukti ◽

Awal Prasetyo ◽

Yan Wisnu Prajoko ◽

Najatullah Najatullah ◽

Neni Susilaningsih

Keyword(s):

Wound Healing ◽

Positive Control ◽

Negative Control ◽

Group Iv ◽

Sprague Dawley ◽

Burn Wound ◽

Group Iii ◽

Burn Wound Healing ◽

Significant Difference ◽

Collagen Density

AIM: This study aims to prove the superior outcome of collagen nanosilver in increasing macrophage and collagen on deep dermal burn wound healing. METHODS: This study was a randomized control trial design. All animals used were male Sprague Dawley rats aged 12–14 weeks old and weighed about 200–250 g. Each rat gets four site injuries and is given treatments. The treatment group includes: I (negative control using NaCl 0.9%), II (positive control using silver sulfadiazine cream), III (treatment with nanosilver collagen cream 1%), and IV (treatment with collagen nanosilver cream 2%). Macrophages counts were assessed using IHC F40/80 and collagen density was assessed using Masson’s trichrome staining. RESULTS: Macrophage on day 6 showed a significant difference as seen in group III and group IV. A significant difference was also obtained between macrophage on day 3 and day 6 from group IV, likewise between collagen between day 3 and day 6 from group III and IV. Collagen nanosilver acts as an antimicrobial and anti-inflammatory agent by increasing the mechanism of activation and migration of macrophages to the site of injury. Moreover, this formula modulated the collagen deposition, which forms the tissue fibers which ultimately provide a conducive environment for wound healing. CONCLUSION: Collagen nanosilver has a superior outcome in the increasing macrophage cell count and collagen density of deep dermal burn wound healing.

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Potential Antiseptic of Rhodomyrtus tomentosa leaves extract on Healing wound in Male Wistar rats

Research Journal of Pharmacy and Technology ◽

10.52711/0974-360x.2021.00548 ◽

2021 ◽

pp. 3143-3149

Author(s):

Endang Sri Purwanti Ningsih ◽

Noorlaila Noorlaila ◽

Ikhwan Rizki Muhammad ◽

Windy Yuliana Budianto

Keyword(s):

Wound Healing ◽

Wistar Rats ◽

Leaf Extract ◽

Wound Care ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Significant Difference ◽

Male Wistar Rats ◽

Rhodomyrtus Tomentosa

Background: The process of wound healing is influenced by various factors such as age, hormones, and wound care. Wound care is done to accelerate wound healing which can be done by various methods, one of them is traditional care. Traditional wound care can use medicinal plants. Rhodomyrtus tomentosa is a medicinal plant that has an antioxidant, anti-inflammatory, antitumor and antibacterial content. Thus this study aims to evaluate the effectiveness of the antiseptic solution of the Rodhomyrtus tomentosa leaf extract on wound healing in male Wistar rats. Method: this research is pure experimental research with post test only control group design. Thirty male white rats were divided into five groups, namely negative control, positive control, Rhodomyrtus tomentosa leaf extract 15%, 30%, and 60%. Rhodomyrtus tomentosa leaf extraction was carried out by maceration method with 70% ethano solvent. The extraction results are divided into 3 concentrations (15%, 30% and 60%). The wound healing process was evaluated by measuring the length of the wound manually from 0 to 10 days in each group. Meanwhile, the number of fibroblast cells was calculated through hematoxylin eosin (HE) staining and observed using an Olympus CX41 microscope with a 10x magnification and objective lens magnification in 3 fields. Result: There was a significant difference in the reduction in wound length (p =< 0,000) between the five experimental groups (Rhodomyrtus tomentosa leaf extract solution 15%, 30% and 60%, negative control and positive control. Solution of rhodomyrtus tomentosa leaf extract accelerated the increase in the number of fibroblasts compared to the negative control group (p = 0.003), but did not make a difference (p = 0.403) with the positive control group. Rhodomyrtus tomentosa leaf extraction solution had the same microscopic effect on the number of fibroblasts with a positive control group given 0.9% NaCl solution. Conclusion: There was a significant difference in the number of fibroblasts between all groups, but no difference in wound healing length.

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Effects of 1% and 3% Mobe Leaf Extract Gel on Socket Wound Healing after Tooth Extraction

Dentika Dental Journal ◽

10.32734/dentika.v24i1.5327 ◽

2021 ◽

Vol 24 (1) ◽

pp. 1-5

Author(s):

Olivia Avriyanti Hanafiah ◽

Denny Satria ◽

Avi Syafitri

Keyword(s):

Wound Healing ◽

Tooth Extraction ◽

Leaf Extract ◽

Alveolar Bone ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

P Value ◽

Gel Treatment

Tooth extraction is a process of removing teeth from the alveolar bone. In wound healing, fibroblast are very important cells. The main purpose of this study was to determine the effect of mobe leaf 1% and 3% extract gel (Artocarpus lakoocha) on fibroblast proliferation in post extraction tooth socket wound healing. This research used 16 samples of wistar rats, divided into 4 groups, a positive control group, a negative control group and a 1% and 3% mobe leaf extract gel group. The left mandibular incisors were extracted, then 1% and 3% gels of mobe leaf extract were applied on day 1 to day 7. Data analysis was calculated using the Kruskal-Wallis test on clinical data and one way ANOVA test for microscopic. The result of the socket wound healing activity test for a good concentration of mobe leaf extract gel was 3%. This research shows significant resultith p-value of 0.018 (< 0.05) on the closure of the socket wound clinically which means the closure of the wound accelerates because of the mobe leaf 3% extract gel treatment. The distance of fibroblast on microscopically shows significant resultith a p-value of 0.002 (< 0.05), which means that there was an enlargement of the distance fibroblast at the socket wound closure with application of mobe leaf 3% extract gel. From the results of the study it can be concluded that mobe leaf 3% extract gel has the best ability to show acceleration the closure of the socket wound either clinically or microscopically.

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Promotion of Dental Pulp Wound Healing in New Zealand White Rabbits’ Teeth by Thai Propolis Product

Journal of Veterinary Dentistry ◽

10.1177/0898756418818891 ◽

2018 ◽

Vol 36 (1) ◽

pp. 17-24 ◽

Cited By ~ 2

Author(s):

Nattriya Likitpongpipat ◽

Somboon Sangmaneedet ◽

Poramaporn Klanrit ◽

Rajda Noisombut ◽

Suttichai Krisanaprakornkit ◽

...

Keyword(s):

Wound Healing ◽

New Zealand ◽

Calcium Hydroxide ◽

Median Number ◽

Positive Control ◽

Negative Control ◽

Histological Evaluation ◽

Dentinal Tubules ◽

Pulp Capping ◽

New Zealand White Rabbits

This study examined and compared wound healing between Thai propolis product and calcium hydroxide paste as pulp-capping agents after partial pulpotomy in New Zealand white rabbits. Forty incisor teeth from 10 rabbits were treated. Thirty-six teeth received class V cavity preparations with partial pulpotomy and application of either propolis or calcium hydroxide paste. Similar cavity preparations were performed in 2 teeth without any capping material as a positive control, whereas 2 teeth without the cavity preparation served as a negative control. Histological evaluation showed that both groups had dentin bridge formation. Dentinal tubules in the dentin bridge were more orderly arranged in the Thai propolis group than in the calcium hydroxide group. Wound healing and the median number of hyperemic blood vessels were not statistically significant different between the 2 groups. Thai propolis product may be used as a pulp-capping agent.

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Development and Characterization of Squalene-Loaded Topical Agar-Based Emulgel Scaffold: Wound Healing Potential in Full-Thickness Burn Model

The International Journal of Lower Extremity Wounds ◽

10.1177/1534734620921629 ◽

2020 ◽

pp. 153473462092162

Author(s):

T. S. Shanmugarajan ◽

N. Kalai Selvan ◽

Varuna Naga Venkata Arjun Uppuluri

Keyword(s):

Wound Healing ◽

Tissue Regeneration ◽

Macrophage Polarization ◽

Negative Control ◽

Skin Tissue ◽

Self Healing ◽

Full Thickness ◽

Burn Wound ◽

Burn Wound Healing ◽

Tissue Defects

Full-thickness burns pose a major challenge for clinicians to handle because of their restricted self-healing ability. Even though several approaches have been implemented for repairing these burnt skin tissue defects, all of them had unsatisfactory outcomes. Moreover, during recent years, skin tissue engineering techniques have emerged as a promising approach to improve skin tissue regeneration and overcome the shortcomings of the traditional approaches. Although previous literatures report the wound healing effects of the squalene oil, in the current study, for the first time, we developed a squalene-loaded emulgel-based scaffold as a novel approach for potential skin regeneration. This squalene-loaded agar-based emulgel scaffold was fabricated by using physical cross-linking technique using lecithin as an emulsifier. Characterization studies such as X-ray diffraction, Fourier-transform infrared spectroscopy, and field emission scanning electron microscopy revealed the amorphous nature, chemical interactions, and cross-linked capabilities of the developed emulgel scaffold. The squalene-loaded emulgel scaffold showed excellent wound contraction when compared with the agar gel and negative control. In case of the histopathology and recent immunohistochemistry findings, it was clearly evidenced that squalene-loaded emulgel promoted faster rate of the revascularization and macrophage polarization in order to enhance the burn wound healing. Moreover, the findings also revealed that the incorporation of squalene oil into the formulation enhances collagen deposition and accelerates the burnt skin tissue regeneration process. Finally, we conclude that the squalene-loaded emulgel scaffold could be an effective formulation used in the treatment of the burnt skin tissue defects.

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New Zealand green-lipped mussels (Perna canaliculus) enhance non-haem iron absorptionin vitro

British Journal Of Nutrition ◽

10.1017/s0007114511006490 ◽

2012 ◽

Vol 108 (6) ◽

pp. 949-952 ◽

Cited By ~ 1

Author(s):

Robin J. C. Stewart ◽

Jane Coad ◽

Gordon W. Reynolds ◽

Kevin C. Pedley

Keyword(s):

Ascorbic Acid ◽

Positive Control ◽

Negative Control ◽

Nutritional Composition ◽

Egg Albumin ◽

Cell Monolayers ◽

Perna Canaliculus ◽

Fe Uptake ◽

Haem Iron

Fe bioavailability can be manipulated by the nutritional composition of a meal. Ascorbic acid and unidentified components of meat, fish and poultry, but particularly beef, all appear to enhance the absorption of non-haem Fe. The aim of the present study is to identify whether extracts of green-lipped mussels (GLM;Perna canaliculus) enhance non-haem Fe absorption in Caco-2 cells and to compare the effect with that of beef. Raw GLM and raw beef homogenates were digestedin vitrowith pepsin at pH 2, and pancreatin and bile salts at pH 7. Tracer55Fe was used to measure cellular Fe uptake. Ascorbic acid was used as a positive control and egg albumin, exposed to the samein vitrodigestion process, was used as a negative control. Caco-2 cell monolayers were incubated with treatments for 60 min. All values were standardised per μg of GLM, egg albumin, beef or ascorbic acid. The results showed that ascorbic acid enhanced non-haem Fe absorption to the highest degree. Beef and GLM digestates both significantly enhanced Fe absorption compared with egg albumin. In conclusion, GLM digestate significantly enhances non-haem Fe uptake in Caco-2 cells with a similar magnitude to that of beef.

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Pharmacological Investigation of the Wound Healing Activity of Cestrum nocturnum (L.) Ointment in Wistar Albino Rats

Journal of Pharmaceutics ◽

10.1155/2016/9249040 ◽

2016 ◽

Vol 2016 ◽

pp. 1-8 ◽

Cited By ~ 6

Author(s):

Hemant Kumar Nagar ◽

Amit Kumar Srivastava ◽

Rajnish Srivastava ◽

Madan Lal Kurmi ◽

Harinarayan Singh Chandel ◽

...

Keyword(s):

Wound Healing ◽

Ethanolic Extract ◽

Negative Control Group ◽

Negative Control ◽

Control Group ◽

Albino Rats ◽

Healing Effect ◽

Wound Model ◽

Wound Healing Effect ◽

Wistar Albino Rats

Objectives. The present study was aimed at investigating the wound healing effect of ethanolic extract of Cestrum nocturnum (L.) leaves (EECN) using excision and incision wound model. Methods. Wistar albino rats were divided into five groups each consisting of six animals; group I (left untreated) considered as control, group II (ointment base treated) considered as negative control, group III treated with 5% (w/w) povidone iodine ointment (Intadine USP), which served as standard, group IV treated with EECN 2% (w/w) ointment, and group V treated with EECN 5% (w/w) ointment were considered as test groups. All the treatments were given once daily. The wound healing effect was assessed by percentage wound contraction, epithelialization period, and histoarchitecture studies in excision wound model while breaking strength and hydroxyproline content in the incision wound model. Result. Different concentration of EECN (2% and 5% w/w) ointment promoted the wound healing activity significantly in both the models studied. The high rate of wound contraction (P<0.001), decrease in the period for epithelialization (P<0.01), high skin breaking strength (P<0.001), and elevated hydroxyproline content were observed in animal treated with EECN ointments when compared to the control and negative control group of animals. Histopathological studies of the EECN ointments treated groups also revealed the effectiveness in improved wound healing. Conclusions. Ethanolic extract of Cestrum nocturnum (EECN) leaves possesses a concentration dependent wound healing effect.

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