PENGEMBANGAN DAN VALIDASI METODE AKRILAMIDA PADA UBI JALAR GORENG SECARA KROMATOGRAFI LAPIS TIPIS-DENSITOMETRI

2021 ◽  
Vol 3 (1) ◽  
pp. 146
Author(s):  
Ayik Rosita P
Keyword(s):  
Retardation Factor

Penelitian ini dilakukan karena sebelumnya belum ada laporan tentang metode kromatografi lapis tipis (KLT) Densitometri untuk analisis akrilamida dalam ubi jalar goreng. Hasil penelitian menggunakan lempeng KLT silika gel 254 menunjukkan bahwa retardation factor berkisar 0,4-0,5 dengan eluen metanol: benzena (2:1) dan dianalisis di panjang gelombang maksimum 200 nm. Linieritas standar akrilamida diuji dalam rentang konsentrasi 120-300 ppm dan koefisien korelasi (r) lebih besar dari 0,99, dengan batas deteksi 17,45 ppm dan batas kuantitatif 52,36 ppm. Persiapan sampel dilakukan dengan cara ekstraksi pelarut menggunakan etanol 70%. Presisi memberikan hasil 2,67% dan nilainya lebih kecil dari 16% sesuai literatur. Persen recorvery 98,18% dan sesuai dengan literature yaitu 98% -102%. Dari penelitian ini dapat disimpulkan bahwa metode KLT Densitometri pada penentuan akrilamida selektif, presisi, dan akurat dimana pada penetapan kadar akrilamida pada ubi jalar goreng mengandung akrilamida sangat sedikit dimana konsentrasinya kurang dari 230,32 ng sehingga sampel ini sangat aman untuk dikonsumsi.

Parameter Determination for Chloride and Tritium Transport in Undisturbed Lysimeters during Steady Flow

1992 ◽  
Vol 23 (2) ◽  
pp. 89-104 ◽  
Author(s):  
Ole H. Jacobsen ◽  
Feike J. Leij ◽  
Martinus Th. van Genuchten
Keyword(s):  
Experimental Data ◽  
Unsaturated Flow ◽  
Transport Model ◽  
Time Moment ◽  
Breakthrough Curves ◽  
Coarse Sand ◽  
Retardation Factor ◽  
Parameter Determination ◽  
Model Parameters ◽  
Water Fraction

Breakthrough curves of Cl and 3H2O were obtained during steady unsaturated flow in five lysimeters containing an undisturbed coarse sand (Orthic Haplohumod). The experimental data were analyzed in terms of the classical two-parameter convection-dispersion equation and a four-parameter two-region type physical nonequilibrium solute transport model. Model parameters were obtained by both curve fitting and time moment analysis. The four-parameter model provided a much better fit to the data for three soil columns, but performed only slightly better for the two remaining columns. The retardation factor for Cl was about 10 % less than for 3H2O, indicating some anion exclusion. For the four-parameter model the average immobile water fraction was 0.14 and the Peclet numbers of the mobile region varied between 50 and 200. Time moments analysis proved to be a useful tool for quantifying the break through curve (BTC) although the moments were found to be sensitive to experimental scattering in the measured data at larger times. Also, fitted parameters described the experimental data better than moment generated parameter values.

Transport of Microorganisms in the Underground – Processes, Experiments and Simulation Models

1991 ◽  
Vol 24 (2) ◽  
pp. 309-314 ◽  
Author(s):  
G. Teutsch ◽  
K. Herbold-Paschke ◽  
D. Tougianidou ◽  
T. Hahn ◽  
K. Botzenhart
Keyword(s):  
Simulation Models ◽  
Mass Conservation ◽  
Breakthrough Curves ◽  
Conservation Equation ◽  
Retardation Factor ◽  
Natural Systems ◽  
Laboratory Setup ◽  
Preliminary Model ◽  
Sand And Gravel ◽  
Mass Conservation Equation

In this paper the major processes governing the persistence and underground transport of viruses and bacteria are reviewed in respect to their importance under naturally occurring conditions. In general, the simulation of the governing processes is based on the macroscopic mass-conservation equation with the addition of some filter and/or retardation factor and a decay coefficient, representing the natural “die-off” of the microorganisms. More advanced concepts try to incorporate growth and decay coefficients together with deposition and declogging factors. At present, none of the reported concepts has been seriously validated. Due to the complexity of natural systems and the pathogenic properties of some of the microorganisms, experiments under controlled laboratory conditions are required. A laboratory setup is presented in which a great variety of natural conditions can be simulated. This comprises a set of 1 metre columns and an 8 metre stainless-steel flume with 24 sampling ports. The columns are easily filled and conditioned and therefore used to study the effects of different soil-microorganism combinations under various environmental conditions. In the artificial flume natural underground conditions are simulated using sand and gravel aquifer material from the river Neckar alluvium. A first set of results from the laboratory experiments is presented together with preliminary model simulations. The large variety of observed breakthrough curves and recovery for the bacteria and viruses under investigation demonstrates the great uncertainty encountered in microbiological risk assessment.

Experimental study and modeling of uranium (VI) transport through ferrous olivine rock columns

2000 ◽  
Vol 88 (9-11) ◽  
Author(s):  
M. Rovira ◽  
F.Z. El Aamrani ◽  
L. Duro ◽  
Ignasi Casas ◽  
Joan de Pablo ◽  
...  
Keyword(s):  
Nuclear Waste ◽  
Reactive Transport ◽  
Calculated Data ◽  
Buffering Capacity ◽  
Retardation Factor ◽  
Reactive Transport Modeling ◽  
Redox Active ◽  
Different Types ◽  
Free Media ◽  
Waste Repositories

The Lovasjärvi intrusion (SE-Finland) contents a high percentage of ferrous olivine (> 65%). This material has been suggested as a redox-active backfill-additive in deep nuclear waste repositories, due to the large Fe(II) proportion in its mineral composition. In order to understand the processes involved in the redox buffering capacity of this material the transport of uranium (VI) through olivine columns was studied. The results showed considerable retardation factor for the U(VI), particularly in carbonate-free media. The experimental data were simulated by means of reactive transport modeling. The best agreement between the experimental and calculated data was obtained considering that the interaction of U(VI) with the olivine surface occurred at two different types of sorption sites. One type accounts for the sorption capacity of the olivine mineral, and a second type accounts for the sorption on amorphous Fe(OH)

Thrombin activatable fibrinolysis inhibitor (TAFI) affects fibrinolysis in a plasminogen activator concentration-dependent manner

2004 ◽  
Vol 91 (03) ◽  
pp. 473-479 ◽  
Author(s):  
Ana Guimarães ◽  
Dingeman Rijken
Keyword(s):  
Plasminogen Activator ◽  
In Vitro Study ◽  
Inhibitory Effect ◽  
Retardation Factor ◽  
Clot Lysis ◽  
Dependent Manner ◽  
Plasma Clot ◽  
Concentration Dependent Manner ◽  
Plasmin Inhibitor

SummaryTAFIa was shown to attenuate fibrinolysis. In our in vitro study, we investigated how the inhibitory effect of TAFIa depended on the type and concentration of the plasminogen activator (PA). We measured PA-mediated lysis times of plasma clots under conditions of maximal TAFI activation by thrombin-thrombomodulin in the absence and presence of potato carboxypeptidase inhibitor. Seven different PAs were compared comprising both tPA-related (tPA, TNK-tPA, DSPA), bacterial PA-related (staphylokinase and APSAC) and urokinase-related (tcu-PA and k2tu-PA) PAs. The lysis times and the retardation factor were plotted against the PA concentration. The retardation factor plots were bell-shaped. At low PA concentrations, the retardation factor was low, probably due to the limited stability of TAFIa. At intermediate PA concentrations the retardation factor was maximal (3-6 depending on the PA), with TNK-tPA, APSAC and DSPA exhibiting the strongest effect. At high PA concentrations, the retardation factor was again low, possibly due to inactivation of TAFIa by plasmin or to a complete conversion of glu-plasminogen into lys-plasminogen. Using individual plasmas with a reduced plasmin inhibitor activity (plasmin inhibitor Enschede) the bell-shaped curve of the retardation factor shifted towards lower tPA and DSPA concentrations, but the height did not decrease. In conclusion, TAFIa delays the lysis of plasma clots mediated by all the plasminogen activators tested. This delay is dependent on the type and concentration of the plasminogen activator, but not on the fibrin specificity of the plasminogen activator. Furthermore, plasmin inhibitor does not play a significant role in the inhibition of plasma clot lysis by TAFI.

scholarly journals Temperature effect in potassium and nitrate ions in soil transport

2008 ◽  
Vol 28 (3) ◽  
pp. 438-447 ◽  
Author(s):  
Adriano D. M. A. Gonçalves ◽  
Jarbas H. Miranda ◽  
Paulo Rossi ◽  
José F. G. Sabadin ◽  
Marcos Y. Kamogawa
Keyword(s):  
Porous Medium ◽  
Dispersion Coefficient ◽  
Potassium Nitrate ◽  
Positive Influence ◽  
Retardation Factor ◽  
Nitrate Transport ◽  
Transport Parameters ◽  
Medium Temperature ◽  
Soil Transport ◽  
Solute Dynamics

When doing researches on solute dynamics in porous medium, the knowledge of medium characteristics and percolating liquids, as well as of external factors is very important. An important external factor is temperature and, in this sense, our purpose was determining potassium and nitrate transport parameters for different values of temperature, in miscible displacement experiments. Evaluated parameters were retardation factor (R), diffusion/dispersion coefficient (D) and dispersivity, at ambient temperature (25 up to 28 ºC), 40 ºC and 50 ºC. Salts used were potassium nitrate and potassium chlorate, prepared in a solution made up of 5 ppm nitrate and 2.000 ppm potassium, with Red-Yellow Latosol porous medium. Temperature exhibited a positive influence upon porous medium solution and upon dispersion coefficient.

Migration of Anionic Species of Radioactive Cobalt through Soil

1989 ◽  
Vol 176 ◽  
Author(s):  
Toshihiko Ohnuki ◽  
David E. Robertson
Keyword(s):  
Concentration Distribution ◽  
Correlation Coefficients ◽  
Ionic Species ◽  
Average Concentration ◽  
Disposal Site ◽  
Retardation Factor ◽  
Laboratory Measurements ◽  
Waste Disposal Site ◽  
Anionic Species ◽  
Cross Correlations

ABSTRACTThe migration of an anionic species of 60Co through soil has been examined utilizing data on the migration of radionuclides leached from an aqueous waste disposal site. Correlation coefficients between concentrations of the anionic species of 60Co and those of the particulate, cationic and non-ionic species of 60Co reveal that the anionic species of 60Co was not interconverted from the other species during migration. The cross correlations of changes in the concentrations of the anionic species of 60Co with time between three different down gradient positions give a calculated retardation factor of the anionic species of 60Co of approximately 19, being 1200 times lower than the results of laboratory measurements. The average concentration distribution of the anionic species of 60Co suggests that the migration of the anionic species of 60Co consists of two migration fractions which were driven by different migration mechanisms.

scholarly journals Dynamics of Ions in Soils Irrigated with Saline Reject

2017 ◽  
Vol 9 (11) ◽  
pp. 190
Author(s):  
Andler Milton Paiva de Oliveira ◽  
Cezar Augusto Medeiros Rebouças ◽  
Nildo Da Silva Dias ◽  
Francisco Souto de Sousa Júnior ◽  
Francisco Vanies da Silva Sá ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Diffusion Coefficient ◽  
Soil Profile ◽  
Water Contamination ◽  
Dispersion Coefficient ◽  
Retardation Factor ◽  
Well Water ◽  
Pollution Potential ◽  
Ground Water Contamination ◽  
Lower Mobility

The objective of this work was to evaluate the variation in the electrical conductivity and the mobilization of ions present in reject brine from desalination plant of brackish well water in three types of soil. The mobilization of the contaminant ions in the reject brine was studied in glass percolation columns, which were filled with soil of contrasting textures (eutrophic CAMBISOL, typic dystrophic Red OXISOL, ENTISOL Quartzipsamment). Experiments ware repeated three times each, and the initial and final concentrations of the ion contaminants were analyzed. The pollution potential of this residue was determined by the retardation factor and dispersion-diffusion coefficient of Ca2+ and Mg2+, besides the variation of electrical conductivity along the profile of each soil studied. In the Red Oxisol, Ca2+ and Mg2+ ions move with greater ease resulting in possibility largest of potential of ground water contamination. In Entisol Quartzipsamment presented higher Mg2+ (R) ion advancement speed, that is, higher subsurface contamination power for these ions. The eutrophic Cambisol presented low diffusion-dispersion coefficient in all the evaluated ions and, therefore, lower mobility of the ions in the soil profile, and consequently, a greater possibility of contamination when irrigated with reject brine.

scholarly journals BENTONITE-QUARTZ SAND AS THE BACKFILL MATERIALS ON THE RADIOACTIVE WASTE REPOSITORY

2010 ◽  
Vol 5 (2) ◽  
pp. 115-120
Author(s):  
Raharjo Raharjo
Keyword(s):  
Radioactive Waste ◽  
Quartz Sand ◽  
Nitrate Solution ◽  
Fixed Bed ◽  
Weight Percent ◽  
Retardation Factor ◽  
Backfill Materials ◽  
Uranium Migration ◽  
Water Saturated ◽  
Bentonite Particle

An investigation of the contribution of quartz sand in the bentonite mixture as the backfill materials on the shallow land burial of radioactive waste has been done. The experiment objective is to determine the effect of quartz sand in a bentonite mixture with bentonite particle sizes of -20+40, -40+60, and -60+80 mesh on the retardation factor and the uranium dispersion in the simulation of uranium migration in the backfill materials. The experiment was carried out by the fixed bed method in the column filled by the bentonite mixture with a bentonite-to-quartz sand weight percent ratio of 0/100, 25/75, 50/50, 75/25, and 100/0 on the water saturated condition flown by uranyl nitrate solution at concentration (Co) of 500 ppm. The concentration of uranium in the effluents in interval 15 minutes represented as Ct was analyzed by spectrophotometer, then using Co and Ct, retardation factor (R) and dispersivity () were determined. The experiment data showed that the bentonite of -60+80 mesh and the quartz sand of -20+40 mesh on bentonite-to-quartz sand with weight percent ratio of 50/50 gave the highest retardation factor and dispersivity of 18.37 and 0.0363 cm, respectively.   Keywords: bentonite, quartz sand, backfill materials, radioactive waste  

Sign in / Sign up

Export Citation Format

Share Document