Effectiveness of Gout Therapy with Polyherbal Extract of Shallot (Allium ascalonicum L.) and Red Ginger (Zingiber officinale var rubrum) in Hyperuricemia Mice

2020 ◽  
Vol 38 (2) ◽  
pp. 135
Author(s):  
Fathur Rohman Haryadi
Keyword(s):  
Uric Acid ◽  
Zingiber Officinale ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Acute Gout ◽  
Ginger Extract ◽  
Oxidase Enzyme ◽  
Red Ginger ◽  
Potassium Oxonate

Gout can cause inflammation of acute gout arthtritis, as well as other complications. Provision of chemical drugs in the long term have side effects. Shallots (Allium ascalonicum L.) contain flavonoids which can inhibit the xanthine oxidase enzyme, so as to reduce uric acid levels. Red ginger extract (Zingiber officinale var rubrum) is rich in gingerol to reduce uric acid with anti-inflammatory effect. Polyberbal combination is expected to increase the effectiveness of therapy. The research aims to determine the effectiveness of polyherbal combinations. 25 male rats (2-3 months) were divided into five groups, namely negative control, positive control, (P1) 25% red ginger extract: 75% shallot, (P2) 50% red ginger extract: 50% shallot, ( P3) 75% red ginger extract: 25% shallot. Induction of potassium oxonate 250 mg / kg BW intraperitoneally on the 7th day. The first measurement of uric acid on the 14th day with Urid Acid Toos, the 15th to 28th days was administered herbal / chemical drugs according to the group. Measurement of second and third uric acid on days 21 and 28. Data were analyzed by one-way Anova (RAL), further significance tests using Least Significant Different (LSD). The results showed that administration of potassium oxonate and combination of herbal extracts had a significant effect (p <0.05) on uric acid levels in all groups. The most effective herbal combination doses are (P3) 75% red ginger (450 mg / 200 gr) and 25% shallot (150 mg / 200 gr BW). The conclusion of this study is the combination of red ginger extract and shallot can be recommended as a uric acid-lowering agent.

scholarly journals Pengaruh Pemberian Kombinasi Ekstrak Etanol Daun Sidaguri (Sida rhombifolia L.) dan Rimpang Jahe Merah (Zingiber officinale Rosc.) pada Mencit Jantan Hiperurisemia

2018 ◽  
Vol 6 (2) ◽  
pp. 205
Author(s):  
Kinanthi Putri Rizki ◽  
Siti Muslichah ◽  
Indah Yulia Ningsih
Keyword(s):  
Uric Acid ◽  
Zingiber Officinale ◽  
Positive Control ◽  
Negative Control ◽  
Male Mice ◽  
Treatment Groups ◽  
Ginger Rhizome ◽  
Red Ginger ◽  
Sida Rhombifolia ◽  
Potassium Oxonate

  This study aimed to determine the effect of the combination of sidaguri leaves and red ginger rhizome extracts in uric acid levels of hyperuricemic male mice. Thirty-six mice were divided into nine groups including normal group, the positive control (+), negative control (-), extracts of four treatment groups with a variety of combinations and two groups of single extract. Hyperuricemia induction was performed by administering mixture melinjo 10% of the standard feed mice for 7 days dan potassium oxonate 250 mg/kg BB intraperitoneally at 2 hours before blood sampling. The results showed that the combination dose of sidaguri leaves of 50 mg/kg BB and red ginger rhizome 400 mg/kg BB had higher antihyperuricemia activity than other combinations in lowering uric acid levels of mice. Compounds may have antihyperuricemic activity were flavonoids, alkaloids, saponins, tannins, and terpenoids.   Keywords: sidaguri leaves, red ginger rhizome, antihyperuricemic activity, uric acid  

scholarly journals Effect of Apium Graveolens (Celery) Seed Extract on Serum Uric Acid Level of Hyperuricemic Rats and its Comparison with Allopurinol

2021 ◽  
Vol 2 (2) ◽  
pp. 85-91
Author(s):  
Abdul Karim ◽  
Shabbir Ali bhatti ◽  
Mahreen Akhtar ◽  
Zartasha Safdar ◽  
Noman Johnson ◽  
...  
Keyword(s):  
Uric Acid ◽  
Serum Uric Acid ◽  
Positive Control ◽  
Negative Control ◽  
Seed Extract ◽  
Control Group ◽  
Lowering Effect ◽  
Celery Seed ◽  
Group B ◽  
Potassium Oxonate

Background: Plant derived medicines are widely used in traditional culture all over the world. Objectives: To determine the effect of Celery Seed Extract (CSE) on uric acid levels in hyperuricemic rats and to compare the effect of allopurinol and CSE. Methods: It was an animal experimental research study. Group A served as negative control whereas Group B served as positive control. CSE was given orally to three groups of rats (C, D, and E). One hour prior to administration of CSE; potassium oxonate was injected intraperitoneally in all groups except negative control to induce hyperuricemia. Similarly, group F was given allopurinol one hour after injection of potassium oxonate. Blood samples were collected for uric acid estimation. Results: It was found that administration of both CSE (group C, D, E) and allopurinol (group F) significantly lowered serum uric acid levels (p<0.001) as compared to positive control (group B). Serum uric acid lowering effect of both drugs CSE and allopurinol was found to be statistically significant on day 3rd and day 7th and was almost comparable. Conclusions: Celery seed extract significantly reduces serum uric acid levels in potassium oxonate-induced hyperuricemic rats and its uric acid lowering effect was comparable with that of allopurinol.

scholarly journals PENGARUH PEMBERIAN EKSTRAK JAHE MERAH (Zingiber officinale var. Rubrum) TERHADAP JUMLAH SEL MAKROFAG ULKUS TRAUMATIKUS MUKOSA MULUT AKIBAT BAHAN KIMIAWI Penelitian in vivo pada Rattus Norvegicus

2015 ◽  
Vol 2 (1) ◽  
pp. 51
Author(s):  
Fitria Hidayati ◽  
Prima Agusmawanti ◽  
Muhammad Dian Firdausy
Keyword(s):  
Oral Mucosa ◽  
Rattus Norvegicus ◽  
Zingiber Officinale ◽  
Positive Control ◽  
Positive Control Group ◽  
Control Group ◽  
Ginger Extract ◽  
Anova Test ◽  
Mucosal Ulceration ◽  
Red Ginger

Background: Traumatic ulcer is an oral mucosa ulcerative lesion caused by trauma. One of the causes is chemicals. Clinical features of traumatic ulcer including yellowish white spots with reddish halo and painful. Treatment for this ulcer are suppressing inflammation and pain thus will increase healing process. Macrophage will be decreased at the end of the inflammatory phase. Red ginger extracts (Zingiber officinale var. Rubrum) contains some compounds that can heal ulcers. Purpose: The objective of this research was to find out the effect of red ginger extract on the macrophage numbers in traumatic oral mucosal ulceration due to chemical in Rattus norvegicus. Method: This study was experimental which post test only control group design. There were five groups: negative control group without intervention, positive control group treat aloclair, and red ginger extract in 2%, 4%, 8% consentration group. The experimental animals were used 25 Rattus norvegicus. They were injured on oral mucosa using H2O2 30% two times a day. Their oral mucosa were made as hystologycal slide, and macrophage counts were observed, then analyzed by Anova test and LSD test. Result: The result indicates some significant effect of red ginger extract on the macrophage numbers in traumatic oral mucosal ulceration from Anova test 0,001 (<0,05). There are significant differences between 2% consentration of red ginger extract and positive control group.There are no significant difference beetween 4%, 8% consentration of red ginger extract and positive control group. Conclusion: It can be concluded that red ginger extract have influence on the number of macrophages in chamical traumatic oral mucosal ulceration in Rattus norvegicus.

scholarly journals PENGHAMBATAN KSANTIN OKSIDASE OLEH KOMBINASI EKSTRAK TEMPUYUNG (Sonchus Arvensis) DAN SALAM (Syzygium Polyanthum) PADA MENCIT HIPERURISEMIA

Biomedika ◽  
2012 ◽  
Vol 5 (1) ◽  
Author(s):  
Dr. Muhtadi . ◽  
Idi Retnani ◽  
Nurcahyanti Wahyuningtyas
Keyword(s):  
Uric Acid ◽  
Xanthine Oxidase ◽  
Oxidase Activity ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Xanthine Oxidase Activity ◽  
Group I ◽  
Sonchus Arvensis ◽  
Potassium Oxonate

Tempuyung (Sonchus arvensis) and salam (Syzygium polyanthum) leaves can lower uric acid levels were studied. The purpose of this research was to determine mechanism of combined o f tempuyung and salam extracts to reduce uric acid levels by inhibiting xanthine oxidase in hyperuricemic mice. This research used completely randomized design using mice. Fifteen mice were made hiperuricemic by 250 mg/kgBW potassium oxonate p.o then the mice were divided into 3 groups. Group I induced 10 mg/kgBW allopurinol p.o as positive control, group II induced tempuyung-salam p.o each 100 mg/kgBW as the treatment group, group th III induced 0.5 mL/20 gBW distilled water p.o as negative control. Liver was taken 4 day after 2 hours induced potassium oxonate. Data inhibition xanthine oxidase activity was tested with Mann-Whitney. The result showed that the combination of tempuyung-salam extract each 100 mg/kgBW could inhibit xanthine oxidase activity of 51,16%±2,76%, while allopurinol 90,20%±0,16% (p = 0.000).Keyword: Hyperuricemia, Xanthine Oxidase, Sonchus arvensis, Syzygium polyanthum

scholarly journals Combination of Propolis Extract (Trigona Sp) and Ginger Rhizome (Zingiber Officinale Roscoe) as Alternative Antibiotics against Escherichia Coli In Vitro

2019 ◽  
Vol 6 (3) ◽  
pp. 112
Author(s):  
Abdul Wahid Jamaluddin ◽  
Muhammad F. Mursalim ◽  
Andi M. S. Apada
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Inhibitory Activity ◽  
Zingiber Officinale ◽  
Positive Control ◽  
Negative Control ◽  
Ginger Extract ◽  
Propolis Extract ◽  
Escherichia Coli Bacteria

Escherichia coli is a gram negative bacterium which is a normal flora in the digestive tract. In birds, this bacterium causes a disease known as colibasillosis. Antibiotics are generally used, but excessive use will cause residues and antibiotic resistance. To avoid resistance or residue, an alternative treatment is needed. The combination of propolis and ginger extract is very promising to develop because both have a synergistic effect as antimicrobials. The research aims to determine the effect of the combination extract on Escherichia coli bacteria in vitro. We used 8 groups which contain combinations of propolis and ginger extract. We used amoxicillin disk as a positive control, 1% Na CMC as a negative control. The results showed a combination of ginger and propolis extract showed good inhibitory activity against Escherichia coli in all groups> 6mm., and the highest inhibitory activity was K3 (5% propolis combination and 15% ginger extract) with 8.7 mm. The combination of propolis and ginger extract has the potential to be used as an alternative antibiotic to prevent antibiotic resistance from synthetic antibiotics.Keywords: alternative antibiotics, combination extracts, Escherichia coli, ginger, in vitro, propolis

scholarly journals UJI AKTIVITAS PENURUNAN KADAR ASAM URAT EKSTRAK ETANOL BUAH PINANG YAKI (Areca vestiaria) PADA TIKUS PUTIH GALUR WISTAR (Rattus norvegicus) YANG DIINDUKSI KALIUM OKSONAT

PHARMACON ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 465
Author(s):  
Christani Rambi ◽  
Edwin De Queljoe ◽  
Herny E. I. Simbala
Keyword(s):  
Uric Acid ◽  
Serum Uric Acid ◽  
Positive Control ◽  
Statistical Test ◽  
Negative Control ◽  
Randomized Design ◽  
Unhealthy Diet ◽  
Group 2 ◽  
Ethanol Extracts ◽  
Potassium Oxonate

ABSTRACTAn unhealthy diet in a high society protein, especially animal protein that contains a lot of purine, causes hyperuricemia (excess uric acid) to increase. The use of allopurinol that is too frequent or excessive  can cause dangerous side effects. Based on these things, it is necessary to do research on antihyperuricemia substances that have high effectiveness and guaranted safety. This study aims to determine the potential for decreasing uric acid levels of areca nut fruit. This type of research as laboratory experiments using a Completely Randomized Design (CRD), with 5 types of treatment each treatment with 3 replications. Group 1 as a negative control was given induction of potassium oxonate, Group 2 as a positive control was given induction of potassium oxonate and allopurinol. Group 3-5 was trated with betel nut extract with different doses of 3,6 mg; 7,2 mg and 14,4 mg. Based on the results of the ANOVA statistical test, serum uric acid levels were significantly different from p = 0,002 (< 0,05). The results of the ethanol extracts of pinangyaki fruit at doses of 3,6 mg / 200gr BW, 7.2mg / 200gr BW and 14,4mg / 200gr BW can reduce serum uric acid levels by anova statistical test with a confidence level of 95%. Keywords: Yaki Areca Fruit, Anthihiperurisemia, Anova ABSTRAKPola makan yang tidak sehat dalam masyarakat yang berprotein tinggi,terutama protein hewani yang banyak mengandung purin, menyebabkan penyakit hiperurisemia (kelebihan asam urat) semakin meningkat. Penggunaan allopurinol yang terlalu sering atau berlebihan dapat menimbulkan efek samping yang berbahaya. Berdasarkan hal-hal tersebut, perlu dilakukan penelitian mengenai zat antihiperurisemia yang memiliki efektivitas tinggi dan terjamin keamanannya . Penelitian ini bertujuan untuk mengetahui potensi aktivitas penurunan kadar asam urat dari Buah Pinang Yaki. Jenis penelitian ini ialah eksperimen laboratorium menggunakan Rancangan Acak Lengkap (RAL), dengan 5 macam perlakuan masing-masing perlakuan dengan 3 ulangan. Kelompok 1 sebagai kontrol negatif diberi induksi kalium oksonat), Kelompok 2 sebagai kontrol positif diberi induksi kalium oksonat dan Allopurinol. Kelompok3-5 diberi perlakuan ekstrak buah pinang yaki dengan dosis yang berbeda-beda yaitu 3,6 mg; 7,2 mg dan 14,4 mg. Berdasarkan hasil uji statistik  Anova didapatkan kadar asam urat serum berbeda secara bermakna dengan p = 0,002 (<0,05). Hasil dari ekstrak etanol buah pinang yaki di dosis 3,6mg/200grBB, 7,2mg/200grBB dan 14,4mg/200grBB dapat menurunkan kadar asam urat serum dengan uji statistik Anova dengan tingkat kepercayaan 95% Kata kunci : Buah Pinang Yaki, Antihiperurisemia, Anova

scholarly journals Antihyperuricemia Activity of the Ethyl Acetate Fraction of Bilimbi Leaves (Averrhoa bilimbi L.)

2021 ◽  
pp. 168
Author(s):  
Fitra Fauziah ◽  
Ifora Ifora ◽  
Silvi Karlila Sari
Keyword(s):  
Uric Acid ◽  
Ethyl Acetate ◽  
Biological Marker ◽  
Ethyl Acetate Fraction ◽  
Positive Control ◽  
Negative Control ◽  
The Body ◽  
Flavonoid Compounds ◽  
Traditional Medicinal Plants ◽  
Potassium Oxonate

Hyperuricemia is a condition where there is an increase in uric acid levels above normal. An increase in the number of leukocytes is also a biological marker of hyperuricemia. The leaves of bilimbi (Averrhoa bilimbi L.) are one of the traditional medicinal plants which contain alkaloids, tannins, steroids, and flavonoid compounds. Flavonoid compounds are thought to inhibit the formation of uric acid in the body. This study investigates the antihyperuricemia activity of the ethyl acetate fraction of bilimbi leaves. The animals were male white mice grouped into six groups: the normal group, negative control, positive control (allopurinol), and the group was given the ethyl acetate fraction of bilimbi leaves at doses of 100, 200, and 400 mg/kg BW. Hyperuricemia induction is by giving high-purine diets and potassium oxonate. The results showed that the variation of the ethyl acetate fraction of bilimbi leaves significantly reduce uric acid levels and the number of leukocyte cells (sig <0.05). Giving ethyl acetate fraction at a dose of 400 mg/kg BW showed the best reduction in uric acid and leukocyte levels.

scholarly journals Effect of Red Ginger (Zingiber officinale Rosc.) Juice on Changes in the Anatomical Pathology of Gills and Amount of Blood Erythrocytes of Koi Fish (Cyprinus carpio koi) Infected by Myxobolus koi

2020 ◽  
Vol 9 (1) ◽  
pp. 1
Author(s):  
Nurul Kumalasari ◽  
Gunanti Mahasri ◽  
Sri Subekti
Keyword(s):  
Design Method ◽  
Zingiber Officinale ◽  
Positive Control ◽  
Negative Control ◽  
Total Blood ◽  
Anatomic Pathology ◽  
Randomized Design ◽  
Sample Test ◽  
Red Ginger ◽  
Cyprinus Carpio Koi

Koi fish commodities have business prospects that can be developed through aquaculture activities in ponds, but in aquaculture, there are often obstacles that are encountered and difficult to control. One obstacle that is difficult to control in the presence of disease attacks caused by parasites. One type of parasite that often attacks koi fish is Myxobolus. Zingiber officinale Rosc. or commonly referred to as red ginger has several properties for the treatment of parasites such as Myxobolus koi, which infects the gills of koi fish. The purpose of this study was to determine the effect of giving red ginger juice (Zingiber officinale Rosc.) To changes in anatomic pathology and the amount of erythrocyte blood of koi fish infected with Myxobolus koi. This study uses a completely randomized design method with six treatments and four replications. The treatments used were different concentrations of red ginger juice that were negative control P1 (healthy fish), positive control P2 (without red ginger juice), P3 (0.1%), P4 (0.2%), P5 (0.3 %) and P6 (0.4%). Pathology scoring data obtained were analyzed using Kruskal Wallis, then followed by an Independent Two-Sample Test, data on the number of erythrocytes obtained were analyzed using ANOVA and then followed by Duncan's Multiple Range Test. The results showed that the administration of red ginger juice gave effect to changes in anatomic pathology and total blood erythrocytes of koi fish infected with Myxobolus. Based on these data, the highest anatomic pathology changes were found in the administration of red ginger concentration of 0.4% with a ranking of 11 and based the on scoring of 2 and the highest average number of erythrocytes found in the treatment of red ginger concentration of 0.4% with an average 14.9 x 105 cells / mm3.

scholarly journals EFEK PEMBERIAN SEDUHAN SERBUK HERBA SELEDRI (Apium graviolens L.) TERHADAP KADAR ASAM URAT SERUM DARAH AYAM LEGHORN JANTAN HIPERURIKEMIA

2021 ◽  
Vol 16 (1) ◽  
pp. 1652-1657
Author(s):  
Ani Kristiyani
Keyword(s):  
Uric Acid ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Oxidase Enzyme ◽  
Effective Doses ◽  
Group 3 ◽  
Group 2 ◽  
Control Group Group

Apium graviolens L. is a medicinal plant that is useful for treating gout. Flavonoids are components of chemical compounds found in celery because they can work as antioxidants and inhibit the action of the xanthine oxidase enzyme to form uric acid. This study aims to determine the effect of giving celery herb powder on the blood serum uric acid levels of hyperuricemia male leghorn chickens. This study used twenty-five male leghorn chickens which were randomly divided into 5 groups. All groups were made hyperuricemia by being given 100% chicken liver juice 5 mL/l kg BW once a day orally during treatment. Group 1 was given 0.5% CMC suspension solution as a negative control group. Group 2 was treated with allopurinol 14 mg/1.5 kg BW in CMC 0.5% as a positive control. Groups 3, 4, 5 received infusion of celery herb powder in the order of doses (0.6 g/1.5 kg BW), (1.2 g/1.5 kg BW), (1.8 g/1.5 kg BW). Examination of uric acid levels was carried out on days 0, 4, 8, 12. The results showed that steeping celery could reduce uric acid levels in proportion to the dose. The greater the dose given, the greater the decrease in uric acid levels. The dose of celery herb group 3 (1.8 g/1.5 kg BW) was the most effective doses because it had the same value as the positive control.

scholarly journals Antibacterial Activity Test of Red Ginger Extract (Zingiber officinale var. Rubrum) Against Streptococcus pyogenes In vitro

2020 ◽  
Vol 3 (1) ◽  
pp. 24
Author(s):  
Samira Assegaf ◽  
Arthur Pohan Kawilarang ◽  
Retno Handajani
Keyword(s):  
Antibacterial Activity ◽  
Streptococcus Pyogenes ◽  
Zingiber Officinale ◽  
Positive Control ◽  
Bacterial Suspension ◽  
Ginger Extract ◽  
Mueller Hinton ◽  
Red Ginger ◽  
Mueller Hinton Broth

Introduction: Red ginger (Zingiber officinale var. rubrum) is known as an antibacterial agent. Streptococcus pyogenes is the most common bacterial cause of pharyngitis in children and adults. Over the years, antibiotic resistance case is increasing. Therefore, further research of other substances to find an bactericidal or bacteriostatic agent is needed. The aim of this study is to determine the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of Z. officinale var. rubrum against S. pyogenes. Methods: Serial dilution test of red ginger extract in Mueller hinton broth (8 concentrations of red ginger extract : 80%, 40%, 20%, 10%, 5%, 2,5%, 1,25%, and 0,625%) and positive control (1 ml bacterial suspension in Mueller hinton broth) was used to determine MIC. MBC was determined by culturing solutions from the previous dilution test into blood agar plate.lResults: MIC of red ginger extract against S. pyogenes could not be determined because the color of red ginger extract affected the turbidity of the dilution test result. MBC of red ginger extract against S. pyogenes was at concentration 20% of red ginger extract.Conclusion: Z. officinale var. rubrum showed antibacterial activity against S. pyogenes.

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