Conventional and Molecular Based Studies on Trichomonas Vaginalis in Quetta, Balochistan

2020 ◽  
Vol 2 (3) ◽  
pp. 52-55
Author(s):  
Shaista Nasir Khan ◽  
Saadullah Jan ◽  
Abdul Samad ◽  
Irfan Shahzad Sheikh ◽  
Ashik Hussain ◽  
...  

This study was designed to investigate the prevailing status of Trichomonas vaginalis, a protozoan parasite causing urogenital tract infections in human beings, in Quetta, Balochistan, Pakistan. As no documented study of this kind has been conducted so far, it was designed to obtain the overall prevalence of T.vaginalis infection. Through conventional wet mount technique, its morphological identification by staining techniques and with PCR confirmation. In addition to investigation of age-wise and gender-wise prevalence of T.v, its correlation with reproductive disorders was also investigated through a questionnaire proforma while taking urine samples. For this purpose six hundred (600) urine samples (300 samples from each gender) were collected from the public sector hospitals located in Quetta city. The study was designed in three age groups as G1: 21-30, G2: 31-40, and G3: 41-50 years of each gender. A total of four samples were found positive microscopically only in female patients, whereas three of these positive samples were confirmed as T. vaginalis by PCR using BTUB9/2 set of primers. The fourth positive sample might be the specie other than T.vaginalis. It is concluded that the overall prevalence of T.v amongst females was observed as 0.1%, while the samples from males were found negative for T.v. so the overall percentage in total samples found as 0.5%.

2020 ◽  
Vol 5 (1) ◽  
pp. 1
Author(s):  
Serajum Munira ◽  
Amzad Hossain ◽  
Meherun Nessa

The present study aims at exploring the extent of linguistic varieties which are issues of gender disparity in our society. Language is the most advanced form of communication possessed only by human beings. Linguistic elements direct our expressions, feelings and thoughts, and languages even help us transmit knowledge, ideas and beliefs from one generation to another. In fact, language is systematic as it follows rules. However, it is believed that men and women have a strong natural tendency to use different patterns of a language. Relationship between language and gender attracted many researchers’ attention, but some critics of mid-twentieth century considered such studies to be purely feministic. With the passage of time, our social structure met considerable changes which influenced our languages. The objective of this project is to give reasonable answers to the questions regarding the gender based speech differences in today’s world. Survey method was applied in order to maintain high standards of research ethics and maintain the research quality. This study engages 384 respondents; among them 51% were male and 49% were female. According to the survey data, 68% of the participants reported that women always tend to use exaggerated expressions and words that can assist in building rapport. Further, it finds out whether there is any difference between speech patterns of different age groups coming from the same gender. By delving into both historic and contemporary documents, this experiment will lead us to widen the scope of research on various ways of communication.


2020 ◽  
Vol 65 (7-8) ◽  
pp. 23-26
Author(s):  
S. Suadkia ◽  
I. V. Podoprigora ◽  
N. V. Yashina ◽  
L. E. Sarukhanova ◽  
E. G. Kravtsov

Uropathogenic Escherichia coli (UPEC) is a serious health problem worldwide. UPEC's multiple drug resistance combined with virulence factors is a cause of serious concern. In childhood, urinary tract infections are of particular importance, since they can occur against the background of long-term unrecognized congenital anomalies of the kidneys and urinary tract. Of the 106 UPEC clinical isolates, 63.2% of cultures were isolated from girls' urine samples and 36.8% from boys' urine samples, which corresponds to a 1.7: 1 ratio. The antibiotic resistance of the isolated UPEC cultures was assessed in relation to 12 antimicrobial drugs. Among the tested cultures, 49% were multidrug-resistant and 20.75% were found to be resistant to imipenem. Phenotypic analysis of antibiotic susceptibility spectrum of uropathogenic E.coli (n=106) indicates a high percentage of occurrence of multi-resistant UPEC strains (49%) and imipenem-resistant UPEC strains (20.75%) among children of all age groups.


2020 ◽  
Vol 21 (16) ◽  
pp. 1672-1686
Author(s):  
Graziela Vargas Rigo ◽  
Tiana Tasca

Female genital tract infections have a high incidence among different age groups and represent an important impact on public health. Among them, vaginitis refers to inflammation of the vulva and/or vagina due to the presence of pathogens that cause trichomoniasis, bacterial vaginosis, and vulvovaginal candidiasis. Several discomforts are associated with these infections, as well as pregnancy complications and the facilitation of HIV transmission and acquisition. The increasing resistance of microorganisms to drugs used in therapy is remarkable, since women report the recurrence of these infections and associated comorbidities. Different resistant mechanisms already described for the drugs used in the therapy against Trichomonas vaginalis, Candida spp., and Gardnerella vaginalis, as well as aspects related to pathogenesis and treatment, are discussed in this review. This study aims to contribute to drug design, avoiding therapy ineffectiveness due to drug resistance. Effective alternative therapies to treat vaginitis will reduce the recurrence of infections and, consequently, the high costs generated in the health system, improving women’s well-being.


Author(s):  
Laxmi Kumari Yadav ◽  
Ram Lochan Yadav

Background: Urinary tract infections (UTI) during pregnancy issued by improper laboratory investigations situate women at risk of several detrimental complications. Untreated asymptomatic UTI (AUTI) may lead to pyelonephritis preterm birth, low birth weight, etc. Urine culture is used to confirm the diagnosis of UTI. Aim of this research was to carry out to find the prevalence of asymptomatic UTI and to establish antimicrobial drug susceptibility of the pathogens resulting asymptomatic UTI in pregnant women attending at tertiary care, Nepal.Methods: The urine samples were collected by mid-stream clean catch method from 600 pregnant women of age 15-45 years (mean age – 28.84 years) visiting for antenatal check up in the Department of Gynaecology and Obstetrics, Chitwan Medical College (CMC), a tertiary care of Nepal. All the urine samples were subjected to bio-physical, biochemical, microscopic, and microbiological analysis. The prevalence of UTI in three trimesters and in different age groups was obtained together with antimicrobial drug susceptibility tests.Results: On overall l51.83% of urine samples revealed with significant bacteriuria considered as the AUTI. On One- way ANOVA test, there was significant (P< 0.045) prevalence of AUTI when compared among different trimesters, though higher prevalence being in the first (40%) and second (45.33%) trimesters. The prevalence of AUTI was significant (P<0.004) among different age groups of the pregnancy. However, the most affectecd age groups were between 21-29 years. Escherichia coli were the most implicated organism (67.8%) followed by Klebsiella pneumonia (21.6%). The gram-negative bacteria (E. coli, K. pneumonia, and K. oxytoca) were more sensitive against Amikacin, Gentamycin, Meropenem, and Nitrofurantointhan. Other drugs whereas; gram positive bacteria (E. feacalis) had high susceptibility with Vancomycin.Conclusions: There was high incidence of AUTI among the pregnant ladies in the study region; therefore, microbial screening of urine of all pregnant women are must and should be included in the routine antenatal checkups to detect the AUTI to cut down and prevent the maternal and obstetric detrimental complications in them.


2003 ◽  
Vol 8 (3) ◽  
pp. 131-147 ◽  
Author(s):  
Gian Vittorio Caprara ◽  
Mariagiovanna Caprara ◽  
Patrizia Steca

Three cross-sectional studies examined stability and change in personality over the course of life by measuring the relations linking age to personality traits, self-efficacy beliefs, values, and well-being in large samples of Italian male and female participants. In each study, relations between personality and age were examined across several age groups ranging from young adulthood to old age. In each study, personality constructs were first examined in terms of mean group differences accrued by age and gender and then in terms of their correlations with age across gender and age groups. Furthermore, personality-age correlations were also calculated, controlling for the demographic effects accrued by marital status, education, and health. Findings strongly indicated that personality functioning does not necessarily decline in the later years of life, and that decline is more pronounced in males than it is in females across several personality dimensions ranging from personality traits, such as emotional stability, to self-efficacy beliefs, such as efficacy in dealing with negative affect. Findings are discussed in terms of their implications for personality theory and social policy.


2020 ◽  
Author(s):  
A. Asgari ◽  
A.A. Parach ◽  
F. Bouzarjomehri ◽  
F. Shirani-Takabi ◽  
A.H. Mehrparvar ◽  
...  

Introduction: Computer Tomography (CT) scans can deliver a relatively high radiation dose to the patient, therefore radiation protection for this modality is paramount. The present study determined the frequency of no abnormality detected (NAD) brain CT scans and probability of cancer induction in different age groups and genders. Methods: In this study, brain CT reports were used to identify any findings as abnormality detected (AD) and others as NAD. Then probability of future leukemia and brain cancer was estimated for different age and gender groups. Results: On average, in 65% of the cases the results were NAD (56% and 76% among males and females, respectively). Among children, 79% of the reports were NAD. The total number of projected brain cancers was 1.8 and 1.3 for males and females, respectively. The number of projected leukemia cases was 0.75 and 0.7 for males and females, respectively. For pediatric patients, brain CT scans can lead to leukemia cases about 4.5 times more often than adults. Conclusion: Brain CT scans can lead to additional cases of brain cancer and leukemia. A significant fraction of brain CTs were NAD (non-pathologic) and could practically be replaced by other radiation-free imaging modalities, especially in pediatric and young patients.


2017 ◽  
Vol 3 (3) ◽  
pp. 328-333
Author(s):  
Safia Mehmood ◽  
Sheema Zia ◽  
Nida Aziz ◽  
Omama Sajid

The AIDS is one of the most complicated health problems of the 21st century and it threatens the world population. AIDS spread at an alarming rate. This was a descriptive study to determine the knowledge, awareness, and practice about HIV among different age groups. The study carried out in Karachi, Pakistan during the period of January 2016-april 2016, in which 82 peoples are participated; 65 peoples having age in between 18-30, people’s lies in 31-40 years, while only 4 peoples having age above 40. From which 91% peoples thought HIV is dangerous virus and 51% peoples having misconception that it cause AIDS on initial stage and does not have any effect on CD4+. Majority of peoples aware about transmission of AIDS through intercourse, and during pregnancy. But the study show the high level of misbelieve also in mode of transmission that HIV transmit through sweat, saliva etc. Majority of the peoples have knowledge that HIV-1 is the most common type found worldwide. Also large number of peoples have knowledge that HIV weak immune system by destroying CD4+ and require long exposure to progress AIDS. Less than 15% of peoples strongly agree that AIDS is common in women. This misconception reflex the lack of knowledge and awareness in the HIV transmission and gender relation. Also majority of peoples thought that lack of knowledge is major barrier in the proper cure of disease. So increasing knowledge by awareness programs; sexual prevention; by starting national testing resources and curing poverty will cured AIDS in Pakistan. Newly drugs are introduced which can stop the progression of AIDS.


2019 ◽  
Vol 3 (2) ◽  

Radiographic Mandibular Indices serve as easy and relatively cheap tools for evaluating bone mineralization. Objectives: To examine the effect of age and gender on three mandibular indices: the panoramic mandibular index (PMI), the mandibular ratio (MR) and the mandibular cortical index (MCI), among Libyan population. Methods: The three indices were measured on 317 digital (OPGs) of adult humans (155 males, 162 females). The sample was divided into six age groups (from 18-25 years through 56-65 years). The measurements were analyzed for interactions with age and sex, using SPSS (Statistical Package for Social Studies) software version no. 22. The tests employed were two way ANOVA, the unpaired T-test and chi-square test. Results: The mean PMI fluctuated between 0.37 s.d. 0.012 and 0.38 s.d. 0.012. among the sixth age groups. One-way ANOVA statistical test revealed no significant of age on PMI. On the other hand gender variation has effect on PMI, since independent sample t-test disclosed that the difference between the male and female PMI means statistically significant. ANOVA test showed that the means of MR among age groups showed a negative correlation i.e. MR mean declined from 3.01 in 18-25 age groups to 2.7 in 55-65 age groups. In contrary, the gender showed no effect on MR according two sample t-test at p> 0.05. In regards with MCI, statistical analysis showed that it affected by age that is C1 was decreasing by age while C2 and C3 were increased by age. Using chi square test the result indicated that there is a significant difference among the different age group and the two genders in MCI readings. Conclusion: PMI was influenced significantly by age but minimally by the gender. MR is not affected by gender but has a negative correlation with age. MCI is affected by both age and gender


2019 ◽  
Vol 98 (5) ◽  
pp. 200-206

ntroduction: Detection and examination of proper number of lymph nodes in patients after rectal resection is important for next treatment and management of patients with rectal carcinoma. There are no clear guideliness for minimal count of lymph nodes, variant recommendations agree on the number of 12 (10−14) nodes. There are situations, when is not easy to reach this count, mainly in older age groups and in patients after neoadjuvant, especially radiation therapy. As a modality for improvement of lymph nodes harvesting seems to be establishing of defined protocols originally designed for mesorectal excision quality evaluation. Methods: The investigation group was formed by patients examined in 2 three-years intervals before and after implementation of the protocol. Elevation in count of harvested lymph nodes was rated generaly and in relation to age groups and gender. Results: The average count of lymph nodes increased from 10 to 15 nodes, in subset of patients whose received neoadjuvant therapy from 7 to al- most 14 nodes. The recommended number of lymph nodes was obtained in all investigated age groups. By the increased number of lymph nodes, rises also possibility of positive nodes found, that can lead to upstaging of the disease, in subset of patients whose received neoadjuvant therapy it is more than 4%. Conclusion: Our conclusions show, that forming of multidisciplinary cooperative groups (chiefly surgeon-pathologist), implementation of defined protocol of surgery, specimen manipulation and investigation by detached specialists lead to benefit consequences for further management and treatment of the patients with colorectal cancer.


2018 ◽  
Vol 6 (1) ◽  
pp. 8
Author(s):  
Mohammad Ahashan ◽  
Dr. Sapna Tiwari

Man has always tried  to determine  and tamper the image of woman and especially her identity is manipulated and orchestrated. Whenever a woman is spoken of, it is always in the relation to man; she is presented as a wife , mother, daughter and even as a lover but never as a woman  a human being- a separate entity. Her entire life is idealized and her fundamental rights and especially her behaviour is engineered by the adherents of patriarchal society. Commenting  on the Man-woman relationship in a marital bond Simone de Beauvoir wrote in her epoch-making book entitled The Second Sex(1949): "It has been said that marriage diminishes man,  which is often true , but almost always it annihilates women". Feminist movement advocates the equal rights and equal opportunities for women. The true spirit of feminism is into look at women and men as human beings. There should not be gender bias or discrimination in familial and social life. To secure gender justice and gender equity is the key aspects of feminist movement. In India, women writers have come forward to voice their feminist approach to life and the patriarchal family set up. They believe that the very notion of gender is not only biotic and biologic episode but it has a social construction.


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