Prevalence of Multiple Drug Resistance among Avian Pathogenic E. coli Isolates from Commercial Poultry

2020 ◽  
Vol 2 (4) ◽  
pp. 83-88
Author(s):  
Abdul latif Baloch
Keyword(s):  
Multiple Drug Resistance ◽  
Polymyxin B ◽  
Economic Losses ◽  
Multiple Drug ◽  
Mortality And Morbidity ◽  
E Coli ◽  
Multiple Organs ◽  
Poultry Birds ◽  
Commercial Poultry ◽  
Tract Infections

Infections associated with Avian Pathogenic E. coli are responsible for huge economic losses for poultry industry worldwide. Particularly, its association with colibacillosis, a complex syndrome which is characterized by lesions of multiple organs i.e. peritonitis, pericarditis, air Sacculitis, osteomyelitis, salpingitis and yolk sac infections is responsible for high mortality and morbidity. Moreover, it causes respiratory tract infections among poultry birds, followed by septicaemia. Liver samples were collected from commercial poultry birds from the various retail shops located in Peshawar City. Bacteria were identified by biochemical and molecular methods. Out of all the tested isolates n=85, 98% were identified as Avian Pathogenic E. coli (APEC). Identified APEC samples were further tested against 23 different antibiotics including amoxicillin (89.40%), levofloxacin (62.40%), ciprofloxacin (71.80%), tobramycin (14.10%), gentamycin (34.10%),neomycin (53.00%), streptomycin (81.00%), tigecyclines (0.00%), oxytetracyclines (96.50%), doxycycline (61.20%), nitrofurantoin (1.00%), chloramphenicol (63.50%), cefixime (7%), cefepime (4.70%), ceftazidime (8.30%), cefotaxime (8.00%), cephalothin (43.50%), trimethoprim, sulfamethoxazole (77.60%), lincomycin (100%), augmentin (4.70%), carbapenem (4%) and polymyxin B (15%). Out of all n=85 isolates 99.9% were multi-drug resistant. Furthermore, ESBL encoding TEM, OXA, SHV were detected in following percentages 53.60%, 19.50%, 9.70% respectively genes.

scholarly journals Patterns of Drug-Resistant Bacteria in a General Hospital, China, 2011–2016

2019 ◽  
Vol 68 (2) ◽  
pp. 225-232 ◽  
Author(s):  
TINGTING MAO ◽  
HUIJUAN ZHAI ◽  
GUANGCAI DUAN ◽  
HAIYAN YANG
Keyword(s):  
General Hospital ◽  
Multiple Drug Resistance ◽  
Polymyxin B ◽  
Resistance Rate ◽  
Resistant Bacteria ◽  
Multiple Drug ◽  
Drug Resistant ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Drug Resistant Bacteria

Drug-resistant bacteria has been a threat to public life and property. We described the trends and changes in antibiotic resistance of important pathogens in a general hospital in Zhengzhou, China from 2011 to 2016, to control antimicrobial-resistant bacteria in hospital and provide support to clinicians and decision-making departments. Five dominant bacteria were enrolled based on the data from the general hospital during 6 years. The results of antimicrobial susceptibility testing were interpreted according to Clinical and Laboratory Standards Institute (CLSI). From 2011 to 2016, a total of 19,260 strains of bacteria were isolated, of which Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Acinetobacter baumannii accounted for 51.98%. The resistance rate of K. pneumoniae and E. coli to carbapenem was less than 15%, but resistance of K. pneumoniae to carbapenems increased with time and resistance of E. coli to meropenem increased. The rate of extended-spectrum beta-lactamase (ESBL) production among K. pneumoniae and E. coli was decreasing. For most antibiotics, the resistance rate of ESBL-positive isolates was higher than that of ESBL-negative isolates, excluding carbapenems and cefoxitin. For S. aureus, the rate of methicillin-resistant S. aureus (MRSA) was stable. Resistance of S. aureus to mostly antibiotics decreased with time. Besides polymyxin B, P. aeruginosa and A. baumannii showed high resistance to other antibiotics. For A. baumannii, the resistance rate to mostly antibiotics was increasing. The bacteria showed high levels of resistance and multiple drug resistance. Continuous surveillance and optimizing the use of antibiotics are essential.

Characterization of novel ybjG and dacC variants in Escherichia coli

2013 ◽  
Vol 62 (11) ◽  
pp. 1728-1734 ◽  
Author(s):  
Dongguo Wang ◽  
Enping Hu ◽  
Jiayu Chen ◽  
Xiulin Tao ◽  
Katelyn Gutierrez ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multiple Drug Resistance ◽  
Multiple Drug ◽  
The Novel ◽  
Conventional Pcr ◽  
E Coli ◽  
Novel Variants ◽  
Restriction Sites ◽  
Genetic Structures

A total of 69 strains of Escherichia coli from patients in the Taizhou Municipal Hospital, China, were isolated, and 11 strains were identified that were resistant to bacitracin, chloramphenicol, tetracycline and erythromycin. These strains were PCR positive for at least two out of three genes, ybjG, dacC and mdfA, by gene mapping with conventional PCR detection. Conjugation experiments demonstrated that these genes existed in plasmids that conferred resistance. Novel ybjG and dacC variants were isolated from E. coli strains EC2163 and EC2347, which were obtained from the sputum of intensive care unit patients. Genetic mapping showed that the genes were located on 8200 kb plasmid regions flanked by EcoRI restriction sites. Three distinct genetic structures were identified among the 11 PCR-positive strains of E. coli, and two contained the novel ybjG and dacC variants. The putative amino acid differences in the ybjG and dacC gene variants were characterized. These results provide evidence for novel variants of ybjG and dacC, and suggest that multiple drug resistance in hospital strains of E. coli depends on the synergistic function of ybjG, dacC and mdfA within three distinct genetic structures in conjugative plasmids.

scholarly journals Epidemiology of Carbapenem-Resistant Enterobacteriaceae Infections: Report from the China CRE Network

2017 ◽  
Vol 62 (2) ◽  
Author(s):  
Yawei Zhang ◽  
Qi Wang ◽  
Yuyao Yin ◽  
Hongbin Chen ◽  
Longyang Jin ◽  
...  
Keyword(s):  
Polymyxin B ◽  
Content Type ◽  
E Coli ◽  
Carbapenem Resistant ◽  
Carbapenemase Gene ◽  
Tertiary Hospitals ◽  
Enterobacteriaceae Infections ◽  
Intra Abdominal Infection ◽  
Tract Infections ◽  
Susceptibility Rate

ABSTRACT Carbapenem-resistant Enterobacteriaceae (CRE) infection is highly endemic in China, but estimates of the infection burden are lacking. We established the incidence of CRE infection from a multicenter study that covered 25 tertiary hospitals in 14 provinces. CRE cases defined as carbapenem-nonsusceptible Citrobacter freundii, Escherichia coli, Enterobacter cloacae, or Klebsiella pneumoniae infections during January to December 2015 were collected and reviewed from medical records. Antimicrobial susceptibility testing and carbapenemase gene identification were performed. Among 664 CRE cases, most were caused by K. pneumoniae (73.9%), followed by E. coli (16.6%) and E. cloacae (7.1%). The overall CRE infection incidence per 10,000 discharges was 4.0 and differed significantly by region, with the highest in Jiangsu (14.97) and the lowest in Qinghai (0.34). Underlying comorbidities were found in 83.8% of patients; the median patient age was 62 years (range, 45 to 74 years), and 450 (67.8%) patients were male. Lower respiratory tract infections (65.4%) were the most common, followed by urinary tract infection (16.6%), intra-abdominal infection (7.7%), and bacteremia (7.7%). The overall hospital mortality rate was 33.5%. All isolates showed nonsusceptibility to carbapenems and cephalosporins. The susceptibility rate of polymyxin B was >90%. Tigecycline demonstrated a higher susceptibility rate against E. coli than against K. pneumoniae (90.9% versus 40.2%). Of 155 clinical isolates analyzed, 89% produced carbapenemases, with a majority of isolates producing KPC (50%) or NDM (33.5%)-type beta-lactamases among K. pneumoniae and E. coli. The incidence of CRE infection in China was 4.0 per 10,000 discharges. The patient-based disease burden in tertiary hospitals in China is severe, suggesting an urgent need to enhance infection control.

scholarly journals Relationship of indole production and antibiotic susceptibility in the Klebsiella bacillus

1975 ◽  
Vol 2 (5) ◽  
pp. 425-429
Author(s):  
D Klein ◽  
J A Spindler ◽  
J M Matsen
Keyword(s):  
Multiple Drug Resistance ◽  
Polymyxin B ◽  
Biochemical Properties ◽  
Negative Control ◽  
Multiple Drug ◽  
University Of Minnesota ◽  
Biochemical Tests ◽  
The University ◽  
Relationship Of ◽  
Indole Production

Of 2,442 Klebsiella strains isolated from clinical specimens at the University of Minnesota hospitals, 18.3% were found to be indole positive. A randomly selected equal number of indole-positive and indole-negative control isolates, characterized by 27 biochemical tests and by serotyping against 72 antisera, were tested against 14 antibiotics. The results indicated a greater incidence of multiple drug resistance among the indole-negative strains among those that produced indole. The organisms in the former group, in comparison to their indole-positive counterparts, were significantly more resistant to nitrofurantoin, tetracycline, chloramphenicol, neomycin, streptomycin, nalidixic acid, and kanamycin. Both groups of organisms were similar in their degree of resistance to ampicillin, carbenicillin, cephalothin, sulfisoxazole, colistimethate, polymyxin B, and gentamicin. The biochemical properties of the two indole groups were essentially identical. Correlation between serotype and multidrug resistance was inapparent.

scholarly journals Biotyping of Multidrug Resistant Campylobacter Jejuni from Poultry and Humans in Northern Region of Ghana

2020 ◽  
Author(s):  
Akosua Bonsu Karikari ◽  
Courage Kosi Setsoafia Saba ◽  
Stephen Wilson Kpordze
Keyword(s):  
Campylobacter Jejuni ◽  
Multiple Drug Resistance ◽  
Multidrug Resistant ◽  
Northern Region ◽  
Phenotypic Correlation ◽  
Multiple Drug ◽  
Domestic Birds ◽  
Commercial Poultry ◽  
Asymptomatic Individuals ◽  
Zoonotic Bacteria

Abstract BackgroundThermophilic Campylobacters are zoonotic bacteria which are universally famous for causing enteritis in humans. They are normally found as commensals in the digestive tract of food animals with poultry being a major reservoir of the pathogenic species. This study evaluated the presence of Campylobacter in poultry (commercial and domestic) and humans (patients and asymptomatic individuals) and characterized strains by biotyping and susceptibility test in the Northern region of Ghana. ResultsOf the 346 poultry and 741 human samples analyzed, 43.1% and 12.9% Campylobacter species were recovered. Isolation frequency from commercial birds was significantly more (50.5%) than domestic birds (33.8%), as incidence in patients and asymptomatic individuals was 15.4% and 9% with a p=0.001. Campylobacter jejuni were recovered from more than eighty percent (84%) of commercial birds and 64% of domestic birds and in humans significantly less strains were observed in patients than asymptomatic individuals (p <0.05). Distribution of biotypes in domestic birds, patients and asymptomatic individuals were comparable with C. jejuni biotype I prevalence and Bioytype II largely in commercial poultry. All strains were resistant to tetracycline; and against erythromycin, 69.3% of jejuni strains were resistant but no resistance was recorded among the non-jejuni strains. Most resistance was noted among patient strains. Imipenem and the aminoglycosides were relatively effective as resistance of 10% and below 20% were obtained. Multidrug resistance was between 94-100% with 60% of strains being resistant to 4 or 5 classes of antibiotics. ConclusionThis study found phenotypic correlation between poultry and human strains coupled with copious isolation of C. jejuni biotypes I and II from commercial and domestic poultry which may well be possible source of threat to public health. None of the endorsed treatment drugs (erythromycin, ciprofloxacin, and tetracycline) can be admitted in this region due to common resistance found among strains against these agents but imipenem and aminoglycosides proved to be reliable as strains showed ample susceptibility. The multiple drug resistance (94-100%) observed in our study and other related investigations in the country call for rapid implementation of the policy on antimicrobial use and resistance which has been launched in Ghana.

scholarly journals Microbiological Evaluation and Antibiotic Susceptibility Pattern of Bacterial Isolates Associated with Some Contaminated Edible Fruits and Vegetables

2021 ◽  
Author(s):  
Mallikarjun Gundappa ◽  
C. Prabhurajeshwar ◽  
Sarfaraz Ahmed ◽  
H.M. Navya ◽  
M. Vijayasarathy ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Pathogenic Bacteria ◽  
Fruits And Vegetables ◽  
Multiple Drug Resistance ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Multiple Drug ◽  
Bacterial Isolates ◽  
Microbial Identification ◽  
E Coli

Background: The present study was undertaken to study the percentage of pathogenic bacteria present in different fruit and vegetable samples available in the market in and around Kalaburagi, Karnataka, South India. Methods: A total of 940 different samples were collected from Kalaburagi, out of which 390 (23.84%) were fruits and 550 (35.27%) were vegetables. The pathogenic bacteria were isolated, by enrichment culture method using peptone water. The bacterial isolates were identified by convention microbial identification procedures. Result: Antibiotic resistant testing by disc diffusion method performed for E.coli, Salmonella and Shigella. Among the pathogens, E. coli (86.50%) of the isolates were resistant to Nalidixic acid while Imipenem and Trimethoprim-Sulfamethoxazole has the lowest resistance (19.84%), Salmonella (86.66%) isolates were resistant to Ciprofloxacin while Norfloxacin has the lowest resistance (4.76%) and Shigella (80.35%) isolates were resistant to Vancomycin while Amoxicillin has the lowest resistant (3.57%). Multiple drug resistance (MDR) was seen in E. coli at (38.88%), Salmonella at (26.66%) and Shigella at (10.71%) accordingly. The study therefore shown that E.coli, Salmonella and Shigella occur in Fruits and vegetables which collected from market place in Kalaburagi, Karnataka India, As per the results, adequate precaution should be taken while handles these fruits and vegetables. The antimicrobial resistance pattern shown by the isolates is an indication that adequate measurement needs to be taken to regulate the drug use in both humans and animals in order to minimize the risk of increasing antimicrobial resistance.

scholarly journals Advanced molecular characterization of enteropathogenic Escherichia coli isolated from diarrheic camel neonates in Egypt

2021 ◽  
Vol 14 (1) ◽  
pp. 85-91
Author(s):  
Momtaz A. Shahein ◽  
Amany N. Dapgh ◽  
Essam Kamel ◽  
Samah F. Ali ◽  
Eman A. Khairy ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Drug Resistance ◽  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Multiple Drug Resistance ◽  
Microbial Pathogens ◽  
Multiple Drug ◽  
Gastrointestinal Infections ◽  
Fecal Samples ◽  
E Coli

Background and Aim: Camels are important livestock in Egypt on cultural and economic bases, but studies of etiological agents of camelid diseases are limited. The enteropathogen Escherichia coli is a cause of broad spectrum gastrointestinal infections among humans and animals, especially in developing countries. Severe infections can lead to death. The current study aimed to identify pathogenic E. coli strains that cause diarrhea in camel calves and characterize their virulence and drug resistance at a molecular level. Materials and Methods: Seventy fecal samples were collected from diarrheic neonatal camel calves in Giza Governorate during 2018-2019. Samples were cultured on a selective medium for E. coli, and positive colonies were confirmed biochemically, serotyped, and tested for antibiotic susceptibility. E. coli isolates were further confirmed through detection of the housekeeping gene, yaiO, and examined for the presence of virulence genes; traT and fimH and for genes responsible for antibiotic resistance, ampC, aadB, and mphA. The isolates in the important isolated serotype, E. coli O26, were examined for toxigenic genes and sequenced. Results: The bacteriological and biochemical examination identified 12 E. coli isolates from 70 fecal samples (17.1%). Serotyping of these isolates showed four types: O26, four isolates, 33.3%; O103, O111, three isolates each, 25%; and O45, two isolates, 16.7%. The isolates showed resistance to vancomycin (75%) and ampicillin (66.6%), but were highly susceptible to ciprofloxacin, norfloxacin, and tetracycline (100%). The structural gene, yaiO (115 bp), was amplified from all 12 E. coli isolates and traT and fimH genes were amplified from 10 and 8 isolates, respectively. Antibiotic resistance genes, ampC, mphA, and aadB, were harbored in 9 (75%), 8 (66.6%), and 5 (41.7%), respectively. Seven isolates (58.3%) were MDR. Real-time-polymerase chain reaction of the O26 isolates identified one isolate harboring vt1, two with vt2, and one isolate with neither gene. Sequencing of the isolates revealed similarities to E. coli O157 strains. Conclusion: Camels and other livestock suffer various diseases, including diarrhea often caused by microbial pathogens. Enteropathogenic E. coli serotypes were isolated from diarrheic neonatal camel calves. These isolates exhibited virulence and multiple drug resistance genes.

scholarly journals Diversity, virulence and antibiogram traits of Escherichia coli recovered from potable water sources in Gharbia, Egypt

2020 ◽  
Vol 18 (3) ◽  
pp. 430-438
Author(s):  
Walid Elmonir ◽  
Etab Mohamed Abo Remela ◽  
Yasmine Alwakil
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Water Samples ◽  
Potable Water ◽  
Multiple Drug Resistance ◽  
Tap Water ◽  
Water Sources ◽  
Multiple Drug ◽  
Well Water ◽  
E Coli

Abstract This study aimed to assess the public health risk of coliforms and Escherichia coli contamination of potable water sources in Egypt. A total of 150 water samples (100 tap and 50 well) were collected from five districts in Gharbia governorate, Egypt. High rates of coliforms contamination were recorded in 52 and 76% of examined tap and well water samples, respectively. E. coli strains were detected in 16% of the water samples (15% tap water and 18% well water; 23.7% rural and 8.1% urban). Rural water sources were 3.5 times more likely to be contaminated than urban sources (P = 0.01). Eight (33.3%) E. coli isolates were Shiga toxin-producing E. coli (STEC). Multiple drug resistance (MDR) was observed for 62.5% of the isolates. Seven (29.2%) E. coli isolates harboured at least one of the extended-spectrum beta-lactamase (ESBL) genes. The majority (87.5%) of the STEC isolates were MDRs and harboured ESBL genes. STEC isolates were significantly more likely to resist six classes of antibiotics than non-STEC isolates. This is the first report of potable water contamination with MDR-STEC in Egypt. This study highlights an alarming public health threat that necessitates preventive interventions for public and environmental safety.

scholarly journals Contamination of Hospital Water Supplies in Gilan, Iran, withLegionella pneumophila, Escherichia coli,andPseudomonas aeruginosa

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Masoumeh Ahmadi Jalali Moghadam ◽  
Hamidreza Honarmand ◽  
Sajad Asfaram Meshginshahr
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Legionella Pneumophila ◽  
Water Samples ◽  
Bacterial Contamination ◽  
Multiple Drug Resistance ◽  
Multiple Drug ◽  
Water Supplies ◽  
E Coli ◽  
Contamination Degree ◽  
Commercial Kit

This study is designed to determine the contamination degree of hospital water supplies withPseudomonas aeruginosa, Legionella pneumophila, andE. coliin Gilan, Iran. Samples were collected directly into sterile containers and concentrated by centrifuge. Half part of any sample transferred to yeast extract broth and the second part transferred to Trypticase Soy Broth and incubated for 3 days. DNA was extracted by using commercial kit. Four rounds of PCR were performed as follows: multiplex PCR for detectingPseudomonas aeruginosa, Integron 1, and Metallo-β-lactamases gene; PCR for detectingLegionella pneumophilaandmipgene separately; PCR for detectingE. coli; and another PCR for detecting whole bacterial presence. Contamination rates of cold, warm, and incubator water samples withP. aeruginosa, were 16.6%, 37.5%, and 6.8% consequently. Degrees of contamination withL. pneumophilawere 3.3%, 9.3%, and 10.9% and withE. coliwere zero, 6.2%, and zero. Total bacterial contamination of cold, warm, and incubator water samples was 93.3%, 84.4%, and 89.0% consequently. Metallo-β-lactamases gene was found in 20.0% of all samples. Contamination degree withP. aeruginosawas considerable and withL. pneumophilawas moderate. Metallo-β-lactamases gene was found frequently indicating widespread multiple drug resistance bacteria. We suggest using new decontamination method based on nanotechnology.

Tentative epidemiologic cut-off value and resistant characteristic detection of apramycin against Escherichia coli from chickens

2019 ◽  
Vol 366 (16) ◽  
Author(s):  
Erjie Tian ◽  
Ishfaq Muhammad ◽  
Wanjun Hu ◽  
Zhiyong Wu ◽  
Rui Li ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Drug Resistance ◽  
Multiple Drug Resistance ◽  
Aminoglycoside Antibiotic ◽  
Multiple Drug ◽  
Broth Microdilution ◽  
E Coli ◽  
Microdilution Method ◽  
Broth Microdilution Method ◽  
Resistant Strains

ABSTRACT Escherichia coli are important foodborne zoonotic pathogens. Apramycin is a key aminoglycoside antibiotic used by veterinarians against E. coli. This study was conducted to establish the epidemiological cut-off value (ECV) and resistant characteristics of apramycin against E. coli. In this study, 1412 clinical isolates of E. coli from chickens in China were characterized. Minimum inhibitory concentrations (MICs) of apramycin were assessed by broth microdilution method. MIC50 and MIC90 for apramycin against E. coli (0.5–256 µg/mL) were 8 and 16 µg/mL, respectively. In this study, the tentative ECV was determined to be 16 µg/mL by the statistical method and 32 µg/mL by ECOFFinder software. Besides, the percentages of aac(3)-IV positive strains ascended with the increase of MIC values of apramycin, and the gene npmA was detected in strains with higher MICs. Sixteen apramycin highly resistant strains displayed multiple drug resistance (100%) to amoxicillin, ampicillin, gentamicin, doxycycline, tetracycline, trimethoprim and florfenicol, while most of them were susceptible to amikacin and spectinomycin. In summary, the tentative ECV of apramycin against E. coli was recommended to be 16 µg/mL.

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