scholarly journals DYNAMICS OF PROTEIN METABOLISM IN COWS WITH PROBIOTIC VETOM 1 BASED ON APATHOGENIC BACILLI

Author(s):  
G. A. Nozdrin ◽  
O. V. Lagoda ◽  
K. E. Surodina ◽  
A. G. Nozdrin ◽  
O. M. Gorshkova

The researchers investigated the effect of new probiotic Vetom 1 based on apathogenic bacilli on the biochemical parameters of blood serum, exactly protein, albumins and urea in lactating cows. The experiment was carried out at OOO “Uchkhoz Tulinskoe”. According to the principle of pair-analogues, the researchers arranged a control group and two experimental groups of cows; each group contained 7 cows. The cows of the 1st experimental group were fed with probiotic Vetom 1 dosed 50 mg/kg once a day in the morning before milking; They received Vetom 1 every day during 5 days, then in a day during a month. The cows from the 2nd experimental group received probiotic Vetom 1 dosed 50 mg/kg once a day in the morning before milking, every day during 30 days. The application of the specimen contributed to a decrease in the concentration of total protein in the blood serum within the physiological norm. The aftereffect caused by Vetom 1 when the specimen was applied 5 days every day, then in a day during a month, the authors observed an increase in the concentration of total protein in the blood serum above the physiological standard on the 180 day of application. The similar effect was not observed when Vetom 1 was applied daily. When using Vetom 1, the authors observed a slight increase in the concentration of albumins in the blood serum, both during the period of application and up to 180 days. The specimen contributes to less prominent increase in the concentration of urea in the blood serum in comparison with the control group. Changes in concentrations of albumins and urea occurred within the physiological norm.

Author(s):  
I. A. Pushkaryev ◽  
N. M. Kostomakhin ◽  
S. V. Burtseva ◽  
N. A. Novikov

The reproductive traits of sows are of paramount importance in the production of high-quality pork. The world experience of pig farming shows the need to solve first of all the feed problem. Diets unbalanced in the content of vitamins restrain the manifestation of genetically inherent high prolificacy of sows. The purpose of the research was to study the influence of feeding the vitamin feed additive “LipoCar” on the reproductive traits of sows and the biochemical parameters of blood serum. The results of research on the introduction of vitamin feed additive “LipoCar” into the diet of pregnant sows have been presented in the article. The experiment has been carried out in the production conditions in JSC “Linevsky pedigree farm” on sows of Large White breed. Pregnant sows have received a basic diet, balanced for all the normalized elements of nutrition. Sows of the experimental group in the composition of the basic diet fed the feed additive “LipoCar”. The dosage of the drug “Lipocar” was 2,1 g/head/day. “Lipocar” has been fed for 20 days. The total duration of the experiment was 3 months. When introducing the vitamin feed additive “LipoCar” into the main diet of sows in the second half of pregnancy the increase in the number of piglets in the litter by 1,6-6,8 % and the weight of the litter – by 5,6-56,2 % (P ≤ 0,001) have been revealed in comparison with the control group of animals. There was a higher content of carotene in the blood serum of sows of the experimental group by 50,0 % (P ≤ 0,01), vitamin A - by 48,7 % (P ≤ 0,05), and total protein – by 4,9 % (P ≤ 0,05).


PLoS ONE ◽  
2021 ◽  
Vol 16 (9) ◽  
pp. e0256661
Author(s):  
Fatima Dikhanbayeva ◽  
Elmira Zhaxybayeva ◽  
Zhuldyz Smailova ◽  
Arman Issimov ◽  
Zhechko Dimitrov ◽  
...  

This study aimed to assess potential feeding effect of camel milk curd mass and its mixes to experimental rat’s blood serum biochemical parameters, enzymatic activity and the peptide toxicity. Fifty healthy male Sprague-Dawley rats were divided into five groups (n = 10 each). Each group was fed with camel milk pure curd mass and its mixes for 16 days. At the end of the experiment, rats were sacrificed to collect the samples from the blood serum. Blood serum biochemical parameters total protein, cholesterol, glucose, albumin, triglycerides; the enzymatic activities of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase were determined on the A25 automatic analyser, and peptide toxicity analysed by the reference method. The statistical data have shown no significant differences in body weight gain in all groups. Total protein decreased in group II, IV, and V; however, it increased in group III compared to the control group. Cholesterol grew up in group II and it slightly increased in group V, dropped in groups III and IV compared to group I result. Glucose increased in groups II, III, IV compared to group I; still, group V results show a slight decrease. Albumin decreased in group IV, yet in group V it increased than the group I result. Simultaneously, groups II and III results were changed with less percentage. Triglyceride grew up in groups II, V, and it dropped significantly in groups III, IV compared to the control group. De Ritis ratio of enzymes in groups II, III, and IV fluctuated between 1.31 and 0.98 IU/L; however, group V demonstrated significant data versus group I. Diets peptide toxicity in all groups was lower than control group data. The experimental results indicated that curd mass from camel milk could be used as a pure or with additives and it did not discover the observed side effects.


2011 ◽  
Vol 27 (4) ◽  
pp. 1463-1468
Author(s):  
B. Jasovic ◽  
J. Stojkovic ◽  
B. Milosevic ◽  
M. Milenkovic ◽  
Z. Spasic ◽  
...  

This work has an aim to explore the effects of the product based on the natural zeolit during the fattening of the lambs and also the effects on contents of calcium, phosphorus and magnesium in blood serum. The experiment lasted 15 days in the closed area of the Agricultural school in Kraljevo, and the lambs were divided into three groups of 15 lambs (Control group -C, Experimental group E1 and E2). They were fed with sheep?s milk, concentrate for lambs? fattening and meadow hay. The lambs in the experimental groups, in contrast to Control group, were fed with the different concentration of the preparation based on the natural zeolit (O1=1%, O2=1.5%), so that the manifested differences would be treated as the resault of the different concentration of the zeolit that was added to the lambs? food.At the end of the experiment the differences in the parameters of the lambs? blood serum were distinguished and especially in the concentration of calcium, phosphorus and magnesium.


2021 ◽  
Vol 22 (3) ◽  
pp. 428-435
Author(s):  
A. A. Ivanovsky ◽  
N. A. Latushkina ◽  
E. Yu. Timkina

The purpose of the research was to study the effect of a phytocomplex containing herbal extracts of R. carthamoides, S. coronata, F. ulmaria on blood biochemistry of milking sows, the development and viability of piglets born by the time of weaning. For the experiment, the animals were divided into an experimental (Phytoadditive) and a control (SPK-2 compound feed) groups, 10 animals each. Phytoadditive in dry form was introduced into the diet of pigs of the experimental group during the sucking period (30 days) daily, once per head per day - 10 grams (individually). Before the beginning and at the end of the experiment, the blood of sows was examined for biochemical parameters. The multiplicity, the quality of the piglets born, the weight of the nest and one pig after birth and before weaning, as well as viability were determined. As the result, it was found that the concentration of the investigated extractives in the Phytoadditive was 13.5 g/kg, of which 10.5 g were ecdysteroids and 3.0 g of flavonoid rutin. Significant changes in the total protein content in the blood compared with the beginning of the experiment were noted in experimental pigs from 60.2±0.5 to 67.3±0.2 g/l (P <0.05) and in the control group from 61.0±0.2 to 66.1±0.1 g/l (P <0.05), the "Ca" content from 2.1±0.01 to 2.7±0.02 mmol/l (P <0.05) in the experimental group and from 2.0±0.01 to 2.5±0.02 mmol/l (P <0.05) in the control groups, while being within the normal range. The amount of albumin significantly increased only in the experimental group from 40.8±1.2 to 49.3±2.8 g/l (P <0.05), in the control from 42.2±2.1 to 46.0±1.5 g/l (P> 0.05). The number of piglets with low viability in the experimental group was 2 times less than in the control group. The viability of piglets in the experimental group by the time of weaning was 92.6 %, in the control ‒ 83.2 %.


Author(s):  
K. V. Kireeva ◽  
N. M. Kostomakhin ◽  
I. A. Pushkaryev ◽  
T. V. Kureninova

Primary changes in the physiological status of animals can be traced in the deviation from the norm of indicators of a number of substances in biological fl uids, since biochemical indicators are a kind of indicator of processes occurring in the body. Having knowledge of hematological changes in the body of cattle, managers and specialists of livestock farms can manage the productivity and health of highly productive animals. The purpose of the research was to determine and analyze the biochemical parameters of the blood of cows in the dry period and at the increasing the milk yield when wet crushed corn grain was introduced into their ration. The research has been carried out on cows of Black-and-White breed in LLC “Agrofi rma “Urozhai” in the Zonal district in the Altai Territory. During the research the prevalence of productivity indicators of cows of the experimental group over analogues from the control group was proved. Thus average daily milk yield for the accounting period (60 days) it was 30,9 kg, which was 5,2 kg more than the control herdmates (P > 0,95). The total productivity of one head was 1541,57 kg of milk in the control group and 1854,69 kg in the experimental group, which was 313,12 kg more (P > 0,95). It has been found that most of the studied biochemical parameters corresponded to the physiological norm. The exception was the increased content of total protein in the blood of lactating cows of the experimental group 5 g/l above the upper limit of the norm (P > 0,999). A low albumin content in the blood of animals of the experimental group has been noted, and this indicator was signifi cantly lower than that in the control group by 3,45 g/l (P > 0,99). An excess of the physiological norm of globulin in the blood of animals of both groups has been found, the indicators of the experimental group signifi cantly exceeded the control group by 9,5 g/l (P > 0,99). There was a low level of chlorides after the increasing the milk yield 88,1 mmol/l in the control group and 89,2 mmol/l in the experimental group, then excess of manganese after the increasing the milk yield was 4,50 mmol/l in the control group and 8,14 mmol/l in the experimental group, which could be associated with the increased content of this element in feed and soil.


2019 ◽  
Author(s):  
O K Gogaev ◽  
A A Abaev ◽  
A R Demurova ◽  
E T Choniashvili

The aim of this research is to study the influence of ozone on the efficiency of incubating quail eggs. The study had been conducted in the period from 2014 to 2018 under the SIE LLC “EcoDom”. In the course of the experiment the Estonian quail egg were served as an object of research. To carry out the experiment a household ozonizer ”Groza” was used. The Eggs of the first “control” group were disinfected with formaldehyde vapors according to the standard method – 35 ml of 37 % formalin solution + 20 ml of tap water + 20 g of potassium permanganate per 1 cubic metre of volume in a special chamber. The other three groups were ozonized. The eggs of the 2nd experimental group were treated for 10 minutes exposure time, the third – 20 minutes and the fourth – 30 minutes, the ozone concentration in all experimental groups was the same and was 10 mg/m3. After treatment, the eggs were placed for brooding. Biochemical parameters of blood serum were determined by the analyzer ”Microlab-300”. Ozonation of hatching eggs contributes to increase crude protein in the quail’s blood serum by 9.37, 9.65 and 8.57 % compared to the control group. Hatching conditional quails was increased by 43.35 % in comparison with the control group.


2021 ◽  
Vol 291 ◽  
pp. 02007
Author(s):  
Elena Ulrikh ◽  
Olga Babich ◽  
Stanislav Sukhikh

During the study, it was found that an increase in the dose of sweet yellow clover extract from 0.5 to 2.5 mg/kg of live weight per day contributed to an increase in the number of: hemoglobin by 1.83 g/l (1.79%), leukocytes by 0.14 thousand 109/l (2.34%), total protein in blood serum of sheep by 0.43 g/l (0.7%), glucose in blood of sheep by 0.18 mmol/l (4.19%), calcium in blood of sheep by 0.14 mmol/l (5.32%), phosphorus in blood of sheep by only 0.08 mg/100 ml (1.6%), contributed to an increase in the live weight of sheep by 220 g (2.18%). In the control group, two sheep out of six got bronchitis. The percentage of sheep with bronchitis was 33.33%. After feed to the sheep different doses of sweet yellow clover extract, the sheep in groups I and II of the experimental groups had one sheep each (16.67%), while in groups III, IV and V there were no sheep with bronchitis. Thus, increasing the sweet yellow clover dosage in sheep’ diet reinforced their immunity. In the control group and in the first experimental group, one sheep out of six came down with dyspepsia. The percentage of sheep with dyspepsia was 16.67%. After feed to the sheep doses of sweet yellow clover extract, there were no dyspeptic diseases in sheep.


Author(s):  
M. V. Chornyi

The paper presents the results of studies on the effect of regrouping of piglets at different ages on the resistance of their organisms, immunological status, productive qualities and safety of young pigs. The work was performed at “Stas” LLC on large white breed pigs and their crossbreeds. For the experiment, three groups of piglets were formed from suckling sows — analogues. In the experiment 60 animals from birth up to two months of age were used, 20 animals in each experimental group. The control group of piglets was raised from birth up to 60 days of age in nests, and then they were moved to the rearing workshop; Experimental 1 group was kept up to 10 days old in nests, and then a one-time rearrangement was performed according to live weight. Piglets from experimental group 2 were regrouped according to live weight at 5, 10, 15, 21, 30 days of age. To assess the natural resistance of piglets due to the above groups, hematological, biochemical (total protein, protein fractions) methods, immunological (immunoglobulins of classes IgA, IgM, IgG), and natural resistance (bactericidal activity of blood serum) were used, serum lysozyme activity (LASK), phagocytic neutrophil activity (FAN), phagocytic index (FI), ethological, zootechnical, mathematical. To assess the natural resistance of piglets the following research methods were used: generally accepted zoo veterinary methods (live weight, morbidity, safety), hygienic (microclimate conditions, sanitary regime), hematological (morphological blood composition), biochemical (total protein, protein fractions), immunological (immunoglobulins of classes IgA, IgM, IgG), natural resistance (bactericidal activity of blood serum), ethological and mathematical methods. Results of work. It was found that when growing piglets, nesting, without moving, caused their growth and development without any noticeable physiological disturbances, both from the blood and ethology. In animals (Experimental-1 groups) which underwent a single regrouping in a 10 day reward, their lag in live weight by 12.65% was recorded, compared with the control, with two and three-time movement (Experimental-2) — by 16.10%. Their SSPs were 28% less and 14.7% lower than in the control piglets. Patients with symptoms of diarrhea were identified in the control: in 3–4 daily reimbursement — 1%, in Experimental-1 — 5%, Experimental-2 — 10–15%. The resistance of young animals to gastrointestinal diseases according to the Melenberg coefficient in the Experimental-2 group was 6.13–7.48, in control group it was 0.34, and the safety did not exceed 80.2%. According to the level of immune status, animals from Experimental-2 group were inferior: by BASK — by 25.15% (28 days of age), by LASK — by 7.56%, by FAN — by 10.7% compared with peers from Experimental-1 groups. By the number of eosinophils (Experimental-2 group), characterizing the stress state of piglets, their decrease was observed within 10–12 days, not more than 5 days — in animals from Experimental-1 group and up to 3 days — from control


2019 ◽  
pp. 62-67
Author(s):  
K. A. Kidun ◽  
A. N. Litvinenko ◽  
T. S. Ugolnik ◽  
N. M. Golubykh ◽  
E. K. Solodova

Objective: to assess the effect of chronic nonspecific stress on changes of the biochemical parameters of the blood serum in male Wistar rats.Material and methods. The experimental study was performed on sexually mature male Wistar rats. The experimental group (n = 71) was exposed to chronic nonspecific stress according to the Ortiz method. The control group of the animals included intact animals (n = 31).Results. The study has revealed changes in the biochemical composition of the blood serum of the rats: increased activity of ALT, LDH, ALP, urea, uric acid, and cholesterol, a decreased level of triglycerides.Conclusion. These changes may indicate the alteration of the level of the intensity of the physiological processes of energy supply in the conditions of chronic stress.


Author(s):  
І. І. Юрченко

Висвітлені результати лабораторних дослідженькрові (сироватки) за низкою гематологічних і біохі-мічних показників від корів, хворих на гнійно-запальніпроцеси в дистальному відділі кінцівок, до та післявикористання інтраваскулярного лазерного опромі-нення крові. Встановлено, що використання ІЛОК ілокально порошку сульфату міді сприяє нормалізаціїзапального процесу. До того ж у сироватці крові ре-єструється вірогідне зниження активності аспара-гінової трансамінази та збільшення (в контрольнійгрупі) лужної фосфатази. Крім того у дослідній групівідмічали зниження, а в контрольній збільшення від-сотка лімфоцитів, а також збільшення гемоглобіну вдослідній та зменшення його в контролі. Laboratory results of blood (serum) for a range of hematological and biochemical parameters of cows sick on inflammatory processes in the distal extremities before and after use of intravascular laser irradiation of blood have been lit. It has been established that the use of locally ILOK and copper sulfate powder contributes to the normalization of the inflammatory process. In the blood serum is achieved a significant reduction of the activity of aspartic trans a minase and alkaline phosphatase is increased (in the control group).Also in the experimental group we saw a decreasing of the percentage of lymphocytes, and in control one - increasing the percentage of lymphocytes, as well as an increasing of hemoglobin in the experimental group and in control group – its reduction.


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