scholarly journals Invivo Effect Analysis of Rhodomyrtus tomentosa Leaf Ethanol Extract Against Escherichia coli

2021 ◽  
Vol 7 (1) ◽  
pp. 83
Author(s):  
Doris Noviani ◽  
Adisty Dwi Treasa ◽  
Ade Zakiya Tasman Munaf ◽  
Sri Winarsih ◽  
Dwi Yuni Nur Hidayati ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Ethanol Extract ◽  
The Body ◽  
Infection Model ◽  
Mice Model ◽  
Toxic Dose ◽  
Puerperal Infection ◽  
Increased Risk ◽  
Escherichia Coli Bacteria ◽  
Rhodomyrtus Tomentosa

Puerperium infection contributed 11 percent of 48.17 percent of maternal mortality during the puerperium period; one of the causes of increased risk of puerperium infection was bacteria Gram-negative Escherichia coli. Puerperium infection occurred because bacteria enter through the vagina, wounds on the insertion of the placenta, and other parts spread to blood throughout the body and damage the spleen, liver, and uterus. This research aimed to determine the effect of Rhodomyrtus tomentosa leaves ethanol extract (Rtl-EE) as an antibacterial on the spleen, liver, and uterus of Escherichia coli -induced puerperal infection model mice. Mice in the postnatal treatment group were induced by Escherichia coli bacteria intravaginally and given different doses of ethanol extract of Rhodomyrtus tomentosa leaves. Examination of bacterial colonies growth used pour plate method with EMBA media. The research results showed that the administration of Rtl-EE decreased the growth of bacterial colonies respectively in the liver 1280, 924, 330 CFU/mL; the uterus 1806, 1180, 874 CFU/mL; and the spleen 712, 112, 774 CFU/mL. Colony growth started to decrease at dose 1 of Rtl-EE (100mg/kg BW) of mice model of Escherichia coli -induced puerperal infection. Rhodomyrtus tomentosa leaves contain active compounds - phenols, flavonoids, saponins, tannins, alkaloids, and triterpenoids as antibacterial. Rtl-EE had an antibacterial effect by reducing the number of bacteria on the spleen, liver, and uterus of Escherichia coli -induced puerperal infection model mice. Further research needs to conduct to examine the toxic dose of Rhodomyrtus tomentosa leaves against Escherichia coli bacteria.

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL BAWANG DAYAK (Eleutherine polmifolia (L.) Merr) TERHADAP Salmonella typhi dan Escherichia coli

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Nofita Nofita ◽  
Keyword(s):  
Escherichia Coli ◽  
Inhibitory Concentration ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Salmonella Typhi ◽  
Skin Infections ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Agar Diffusion ◽  
Escherichia Coli Bacteria

Dayak onion (Eleutherine polmifolia (L.) Merr) was a typical plant of Kalimantan. Empirically dayak onion (Eleutherine polmifolia (L.) Merr) used by the comunity to treat skin infections. The purpose of this study was to determine the antibacterial activity of ethanol extract of dayak onion power against Salmonella typhi and Escherichia coli bacteria. The highest concentration used was 100%, and the lowest concentration was 0,5%. As a control of cloramfenikol for Salmonella typhi and ciprofloxacin for Escherichia coli bacteria. This research method used agar diffusion. The results showed the ethanol extract of dayak onion (Eleutherine polmifolia (L.) Merr) contained flavonoids, alkaloids, tannins and phenols with the highest levels was by tannins at 7,45%. Dayak onion (Eleutherine polmifolia (L.) Merr) are inhibiting the growth of Salmonella typhi bacteria at a minimum inhibitory concentration (MIC)of 1% with a inhibition zone diameter of 6,24 mm and 0,9% with a inhibition zone diameter of 6,20 mm at Escherichia coli bacteria.Keywords : Eleutherine polmifolia (L.) Merr, Salmonella typhi, Escherichia coli, Agar diffusion, MIC

scholarly journals The Effect of Various Concentrations of Ethanol Extract of the Leaves of Paederia foetida L. on the Growth of Escherichia Coli Bacteria

2021 ◽  
Vol 11 (6) ◽  
pp. 61-67
Author(s):  
Hertina Silaban
Keyword(s):  
Escherichia Coli ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Rare Plants ◽  
E Coli ◽  
Paederia Foetida ◽  
Escherichia Coli Bacteria ◽  
The One

Bacterial infection of Escherichia coli (E. coli) as the cause of gastrointestinal disorders in humans has increased their prevalence. Treatment using natural ingredients can be a choice of therapy because of the minimal side effects. One of the rare plants believed by the community as an antibacterial is stinking vin’e known as the ‘leaf fart’. The purpose of this research is for knowing the activity of the ethanol extract of Paederia foetida L can affect the growth of E.coli. The serial diffusion disc method is being used as the antibacterial activity test. The concentration  of this extract are 10%, 20%, 40%, 80%, 100% with positive control (ciprofloxacin) and negative control (aqua dest). The inhibition zone diameter characterized the effect of Extract on bacterial growth were 6.16 mm of the concentration 10%, 6.667 mm of the concentration 20%, 7.10 mm of the concentration 40 %, 7.78 mm of the concentration 80%, and 10.03 mm of the concentration 100%. As for the negative control has no effect. The study stated that the higher concentration of antibacterial agent used, the greater the inhibition zone formed. Based on the result of the analysis of the data by using the One-Way ANOVA Test showed a probability value (p) = 0.000 or value (p) < 0.05, that H0 is rejected and H1 is accepted. The conclusion is that the Extract of stinking vin’e has an antibacterial effect on the growth of E.coli. Keywords: Antibacterial, E.coli, Extract of  Sembukan leaf

scholarly journals UJI AKTIVITAS ANTIBAKTERI DAUN JERUK KALAMANSI (Citrofortunella microcarpa) TERHADAP BAKTERI Staphylococcus aureus DAN Escherichia coli

Alotrop ◽  
2019 ◽  
Vol 3 (2) ◽  
Author(s):  
Deza Oktasila ◽  
Nurhamidah Nurhamidah ◽  
Dewi Handayani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Raw Material ◽  
Diffusion Method ◽  
Citrus Leaves ◽  
Escherichia Coli Bacteria

This study aims to examine the antibacterial activity of ethanol extract and essential oil of Kalamansi citrus leaves (Citrofortunella microcarpa) against Staphylococcus aureus and Escherichia coli bacteria. The raw material of Kalamansi citrus leaves is obtained from the Village of Pondok Kubang, Bengkulu Tengah (3.70060S, 102.35780E). Ethanol extract from Kalamansi citrus leaves was obtained by maceration using ethanol 96%, then ethanol extract was made dilution concentration 40 ; 20; , 10; and 5%. The essential oil of Kalamansi citrus leaves is obtained by water-vapor distillation, then made variations of concentration 20;, 15;, 10;  and 5%. The method used to test the antibacterial activity is the paper disc diffusion method , the antibacterial activity is shown by the diameter of the inhibiting  zone formed. The data of antibacterial test result were analyzed by using One Way Anova test which showed the effect of treatment on the growth of test bacteria seen from the value (P <0,01) and continued by Duncan test to know the effect of the treatment. The results showed that ethanol extract of Kalamansi citrus leaves had antibacterial activity against S. aureus and E. coli bacteria with moderate inhibitory diameter is  7.20 and 5.73 mm at concentration 40%, while antibacterial activity on essential oil of Kalamansi citrus leaves is categorized as strong with inhibition zone diameter is 14.83 and 13.00 mm at concentration 20%.

scholarly journals PENENTUAN AKTIVITAS EKSTRAK ETANOL CABAI MERAH DALAM MENGHAMBAT PERTUMBUHAN BAKTERI

2021 ◽  
Vol 26 (1) ◽  
pp. 64-73
Author(s):  
Alfi Sapitri ◽  
Eva Diansari Marbun ◽  
Ulfayani Mayasari
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Clear Zone ◽  
Capsicum Annum ◽  
Activity Test ◽  
Mueller Hinton ◽  
Mueller Hinton Agar ◽  
Escherichia Coli Bacteria

Penelitian ini bertujuan untuk melakukan uji fitokimia terhadap ekstrak etanol Cabai merah (Capsicum annum L.) dan uji aktivitasnya terhadap pertumbuhan bakteri Staphylococcus aureus dan Escherichia coli. Pengujian aktivitas antibakteri ini menggunakan metode difusi agar dengan menggunakan kertas cakram dengan tiga kali pengulangan. Ekstrak etanol cabai merah dibuat dengan konsentrasi 45, 60, 75, dan 90%. Uji aktivitas antibakteri dilakukan dengan metode Kirby Bauer. Media yang digunakan sebagai uji antibakteri pada penelitian ini yaitu Mueller-Hinton Agar (MHA). Hasil skrining fitokimia terhadap ekstrak etanol cabai merah menunjukkan adanya senyawa metabolit sekunder. Senyawa metabolit sekunder tersebut yaitu alkaloid, flavonoid, tanin, saponin, dan Steroid/triterpenoid. Steroid berfungsi sebagai antimikroba. Hasil penelitian ini juga mengungkapkan adanya kandungan senyawa capsaicin yang terkenal sebagai senyawa aktif antimikroba. Ekstrak etanol cabai merah memiliki aktivitas antibakteri terhadap bakteri Staphylococcus aureus dan Escherichia coli zona bening terendah pada konsentrasi 45% dan zona bening tertinggi pada konsentrasi 90%. Dapat disimpulkan bahwa ekstrak cabai merah memiliki aktivitas antibakteri terhadap penghambatan pertumbuhan bakteri.THE ABILITY OF RED CHILI ETHANOL EXTRACT IN INHIBITHING THE GROWTH OF BACTERIAThis study aimed to perform a phytochemical test on the ethanolic extract of red chili (Capsicum annum L.) and test its activity against the growth of Staphylococcus aureus and Escherichia coli bacteria. This antibacterial activity test used the agar diffusion method using disc paper with three repetitions. Red chili ethanol extract was made with concentrations of 45%, 60%, 75% and 90%. The antibacterial activity test was carried out using the Kirby Bauer method. The medium used as an antibacterial test in this study was Mueller-Hinton Agar (MHA). The results of phytochemical screening of red chili ethanol extract showed the presence of secondary metabolites. The secondary metabolites are alkaloids, flavonoids, tannins, saponins, and steroids/triterpenoids. Steroids serve as  antimicrobials. The results of this study also revealed the presence of capsaicin compounds which are known as active antimicrobial compounds. Red chili ethanol extract had antibacterial activity against Staphylococcus aureus and Escherichia coli bacteria in the lowest clear zone at a concentration of 45% and the highest clear zone at a concentration of 90%. It can be concluded that red chili extract has antibacterial activity against bacterial growth inhibition.

UJI AKTIVITAS ANTIBAKTERI DARI EKSTRAK ETANOL DAUN PERIA LAUT (Colubrina Asiatica L.) TERHADAP BAKTERI STAPHYLOCOCCUS AUREUS DAN ESCHERICHIA COLI

2019 ◽  
Vol 2 (1) ◽  
pp. 14-18
Author(s):  
Firdaus Fahdi ◽  
Harwitavia Harwitavia ◽  
Herviani Sari
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Antibiotic Drugs ◽  
Escherichia Coli Bacteria

The discovery of new antibiotic drugs is getting more and more reactive. The plant of the peria laut leaf is one of the drugs that is often used as a traditional medicine and contains bioactive compounds of polyphenols, flavonoids, and saponins, which can inhibit antibacterial growth. Purpose of this study was to determine the antibacterial activity of ethanol extract of peria laut leaves (Colubrina asiatica L.) against Staphylococcus aureus and Escherichia coli bacteria. Method the experimental, of the sample used was concentrated marine peria laut leaf extract of 25mg/ml, 50mg/ml, 75mg/ml, 100mg/ml, 200mg/ml, 300mg/ml, 400mg/ml, and 500mg/ml, positive control of amoxicillin tablet 500 mg, negative control of dimethylsulfoxide with the method of disc diffusion testing using media Nutrient Agar. Results the showed thet the peria laut leaf extract positively contained bioactive alkaloid compounds, flavonoids, saponins, steroids, and tannins, and had inhibitory effects on Staphylococcus aureus and Escgerichia coli bacteria with various concentrations which had been tested on the average area of the highest inhibition zone of 18,6 mm in Staphylococcus aureus bacteria, and 10,2 mm in Escherichia coli bacteria. Conclusion peria laut leaf extract (Colubrina asiatica L.) has antibacterial activity against Staphylococcus aureus and Escherichia coli bacteria in the most inhibitory zone at a concentration of 500mg/ml with a diameter of 18,6 in Staphylococcus aureus bacteria and 10,2 in Escherichia coli.

POTENT ANTIBACTERIAL OF GEL KAWANG FRUITS (Litocarpus celebicus, (Miq) Rehder) ETHANOL EXTRACT ORIGIN FROM PAPUA INDONESIA

2017 ◽  
Vol 10 (14) ◽  
pp. 122
Author(s):  
Soraya Ratnawulan Mita ◽  
Sri Agung Fitri Kusuma ◽  
Nuraini Insiyah
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Ethanol Extract ◽  
The Body ◽  
Diffusion Method ◽  
Test Results ◽  
Native Plant ◽  
Inhibition Concentration ◽  
Gel Preparation ◽  
Better Than

Objective: Kawang fruit (Lithocarpus celebicus (Miq.) Rehder) is native plant of Digul-Bouven, Papua, Indonesia have flavonoids which has antibacterial activity. Antiseptic is a substance used to inhibit the growth or destroy microorganisms that live on the surface of the body. Their resistance to antiseptics on the market, it needed a new antiseptic-based natural ingredients that are relatively safer. The purpose of this study to make an antiseptic gel with extract ethanol of kawang fruit and determine its effectiveness against Staphylococcus aureus ATCC 29 213 and Escherichia coli ATCC 25922.Methods: extraction, phytochemical screening, testing activities with the agar diffusion method, determination Minimum Inhibition Concentration (MIC) and Minimum Bactericidal Concentration (MBC), antiseptic gel formulation, and test preparation gel. The test results showed the kawang fruit has activity against bacteria with value MIC and MBC on Staphylococcus aureus ATCC 29213 is 5% (b/b) and 10% (b/b), and on Escherichia coli ATCC 25922  is 10% and 20%.The extraction is formulated in the form of a gel with a concentration of 5%, 10%, and 20%. Formulations made using 2% HPMC.Results: The test results show the effectiveness of antiseptic gel extracts have inhibitory better on Staphylococcus aureus ATCC 29213 than Escherichia coli ATCC 25922.Conclusion: Antiseptic gel preparation of kawang fruit ethanol extract at a concentration of 5% have shown their antibacterial effectivity. Its inhibition to Staphylococcus aureus ATCC 29213 was better than Escherichia coli ATCC 25 922.

scholarly journals UJI AKTIVITAS ANTIMIKROBA KARANG LUNAK Dendronephtya sp., YANG DIKOLEKSI DARI DESA TUMBAK KECAMATAN PUSOMAEN, KABUPATEN MINAHASA TENGGARA TERHADAP Escherichia coli, Staphylococcus aureus, dan Candida albicans

PHARMACON ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 63
Author(s):  
Gloria Ekaputri Silap ◽  
Defny Wewengkang ◽  
Henki Rotinsulu
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Soft Coral ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Solvent Fractionation ◽  
Escherichia Coli Bacteria ◽  
Partition Method

ABSTRACTSoft coral Dendronephtya sp., living in the waters of coral reefs that are rather deep, at depths below 10 meters, grow attached to a hard substrate, protected under lumps of living coral or dead coral. This study aims to determine the antimicrobial activity of soft coral Dendronephtya sp., collected from the waters of Tumbak Village, Pusomaen District, Southeast Minahasa Regency, against Escherichia coli, Staphylococcus aureus and Candida albicans. Dendronephtya sp., extracted by maceration method using 96% ethanol solvent, fractionation using liquid-liquid partition method with n-hexane, chloroform and methanol solvent, and the testing of antimicrobial activity using the Kirby Bauer diffusion method. The results showed that ethanol extract of soft corals (Dendronephtya sp.), n-hexane fraction had inhibition of 8 mm against Escherichia coli bacteria, 7 mm against Staphylococcus aureus while Candida albicans had very good activity in n-hexane fractions of n-hexane. 9.3 mm, so it can be recommended as an antimicrobial. Keywords : Antimicrobial, Candida albicans, Escherichia coli, Soft Coral (Dendronephtya sp.), Staphylococcus aureus. ABSTRAKKarang lunak Dendronephtya sp., hidup di perairan terumbu karang yang agak dalam, pada kedalaman di bawah 10 meter, tumbuh melekat di dasar yang keras, terlindung di bawah bongkahan karang hidup atau karang mati. Penelitian ini bertujuan untuk mengetahui adanya aktivitas antimikroba dari karang lunak Dendronephtya sp., yang dikoleksi dari perairan Desa Tumbak Kecamatan Pusomaen Kabupaten Minahasa Tenggara terhadap Escherichia coli, Staphylococcus aureus dan Candida albicans. Dendronephtya sp., diekstraksi dengan metode maserasi menggunakan pelarut etanol 96%, fraksinasi menggunakan metode partisi cair-cair dengan pelarut n-heksan, kloroform dan metanol, dan pengujian aktivitas antimikroba menggunakan metode difusi agar Kirby Bauer. Hasil penelitian menunjukkan bahwa ekstrak etanol karang lunak (Dendronephtya sp.) Fraksi karang n-heksan memiliki daya hambat sebesar 8 mm  terhadap bakteri Escherichia coli, 7 mm pada Staphylococcus aureus  sedangkan  Candida albicans aktivitas  yang sangat baik terjadi pada fraksi dan ekstrak n-heksan sebesar 9,3 mm, sehingga dapat direkomendasikan sebagai antimikroba. Kata Kunci : Antimikroba, Candida albicans, Escherichia coli,  Karang Lunak (Dendronephtya sp.), dan Staphylococcus aureus

scholarly journals AKTIVITAS ANTIMIKROBA EKSTRAK DAN FRAKSI ALGA (Halimeda opuntia) TERHADAP Escherichia coli, Staphylococcus aureus dan Candida albicans

PHARMACON ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 178
Author(s):  
Shinta Jolanda ◽  
Defny S Wewengkang ◽  
Imam Jayanto
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Green Algae ◽  
Bioactive Compounds ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Agar Diffusion Method ◽  
Escherichia Coli Bacteria

ABSTRACT Algae Halimeda opuntia is one of the green algae type that contains bioactive compounds which can be used for the treatment of various diseases. This study aimed to determine the antimicrobial activity of extracts and fractions of algae Halimeda opuntia obtained from the Lembeh Bitung Strait against Escherichia coli, Staphylococcus aureus and Candida albicans. The samples were extracted by maceration using ethanol solvent and fractionation using methanol, chloroform, and n-hexane solvents. Antimicrobial activity was carried out by the agar diffusion method of paper discs. The results showed that ethanol extract was able to inhibit the growth of Staphylococcus aureus and Escherichia coli bacteria in the medium inhibitory category. While the methanol and chloroform fractions were only able to inhibit the growth of Staphylococcus aureus bacteria. The extract and all fractions did not show activity inhibiting the growth of Candida albicans fungi. Keywords: Algae, Halimeda opuntia, antimicrobial activity, Escherichia coli, Staphylococcus aureus, Candida albicans ABSTRAKAlga Halimeda opuntia merupakan salah satu jenis alga hijau yang memiliki kandungan senyawa bioaktif yang dapat digunakan untuk pengobatan berbagai penyakit. Penelitian ini bertujuan untuk mengetahui adanya aktivitas antimikroba dari ekstrak dan fraksi alga Halimeda opuntia yang diperoleh dari Selat Lembeh Bitung terhadap Escherichia coli, Staphylococcus aureus dan Candida albicans. Sampel diekstraksi dengan metode maserasi menggunakan pelarut etanol dan fraksinasi menggunakan pelarut metanol, kloroform, dan n-heksan. Aktivitas antimikroba dilakukan dengan  metode difusi agar cakram kertas. Hasil penelitian menunjukkan bahwa ekstrak etanol, mampu menghambat pertumbuhan bakteri  Staphylococcus aureus dan Escherichia coli dengan kategori daya hambat sedang. Sedangkan  fraksi metanol dan fraksi kloroform hanya mampu menghambat pertumbuhan bakteri Staphylococcus aureus. Ekstrak dan semua fraksi tidak menunjukkan aktivitas menghambat pertumbuhan jamur Candida albicans. Kata kunci: Alga, Halimeda opuntia, aktivitas antimikroba, Escherichia coli, Staphylococcus aureus, Candida albicans

scholarly journals Aktivitas Antibakteri Ekstrak Daun Widuri Terhadap Escherichia Coli Dan Staphylococcus Aureus

2020 ◽  
Vol 2 (1) ◽  
pp. 1-9
Author(s):  
Siti Zamilatul Azkiyah
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bacterial Infections ◽  
Leaf Extract ◽  
Antibacterial Properties ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
The Body ◽  
And Staphylococcus Aureus

Antibiotics have a very important role in fighting bacterial infections in the body. However, the results given so far have not been optimal in overcoming the problem of bacterial infection. An alternative solution is to develop and study plants that have medicinal potential, for example thistle leaves (Calotrophis gigantean). The purpose of this study was to determine the antibacterial activity of thistle leaf extract against Escherichia coli and Staphylococcus aureus. This research is a laboratory-scale experimental research that is to test the activity of thistle leaf extract against Escherichia coli and Staphylococcus aureus including sample identification, extraction, activity testing by observing the inhibition zone of the two tested bacteria. Based on the results of the study stated that the ethanol extract of 70% Widuri leaves (Calotrophis gigantea) had antibacterial activity and could inhibit the growth of Escherichia coli and Staphylococcus aureus bacteria. The active compounds that are thought to have antibacterial properties in the ethanol extract of Widuri leaves (Calotrophis gigantea) are flavonoids, alkaloids and terpenoids.   Keywords: Widuri leaves, Escherichia coli, Staphylococcus aureus, Antibacterial ABSTRAK   Pentingnya penanggulangan infeksi bakteri menggunakan antibiotik adalah hal yang lazim dalam terapi farmakologi. Dampak dari pemberian antibiotik masih belum maksimal dalam penanggulangannya terhadap infeksi bakteri. Pemilihan alternatif bahan alam dengan kandungan kimia yang memiliki potensi sebagai antibiotik dapat menjadi pilihan lain dalam mengurangi penggunaan antibiotik sintetik, misalnya tanaman widuri (Calotrophis gigantean). Tujuan dari penelitian ini yaitu untuk mengetahui aktivitas antibakteri ekstrak daun widuri terhadap Escherichia coli dan Staphylococcus aureus. Desain penelitian meliputi identifikasi sampel, ekstraksi, hingga uji aktivitas dengan mengamati zona hambat dari kedua bakteri uji. Hasil penelitian menunjukkan bahwa ekstrak etanol 70% daun Widuri memiliki aktivitas antibakteri dan dapat menghambat pertumbuhan bakteri Escherichia coli dan Staphylococcus aureus. Senyawa aktif yang diduga bersifat antibakteri pada ekstrak etanol daun Widuri adalah senyawa golongan flavonoid, alkaloid dan terpenoid. Kata Kunci: Daun Widuri, Escherichia coli, Staphylococcus aureus, Antibakteri

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