scholarly journals The effect of sulfur compounds on the content of microelements in tissue organism rabbits

2019 ◽  
Vol 21 (95) ◽  
pp. 161-165
Author(s):  
A. Z. Dychok-Nedzelska ◽  
Ya. V. Lesyk ◽  
I. I. Kovalchuk

The aim of the study was to investigate the effect of nanotechnology and sodium sulfate on the content of mineral elements in the tissues of 110-day-old rabbits by feeding different amounts of nano sulfur citrate. Studies were conducted on young rabbits of the Hyla breed in the private sector. Rabbits for the study were selected at the age of 41 days on the principle of analogues, weighing 1.2–1.4 kg, were divided into six groups (control and five experimental), 6 animals (3 males and 3 females) in each. Animals were kept in rooms with adjustable microclimate and illumination in mesh cages measuring 50×120×30 cm, in accordance with modern animal health standards. The rabbits of the control group were fed without restriction a balanced granular compound feed, with free access to water. Animals of the first (I), second (II), third (III), and fourth (IV) experimental groups were fed a control group diet and fed with nano sulfur citrate for 2; 4; 8 and 12 mg S/kg body weight. A solution of nano sulfur citrate (1.0 g/dm3, pH 1.38) was obtained from the Nanomaterials and Nanotechnologies LLC, Kyiv. The young of the fifth (V) experimental group was fed with a  diet of the control group and water was given sodium sulfate (Na2SO4) in the amount of 40 mg S/kg body weight. At the 58th day from the beginning of the experiment, the animals were slaughtered, taking into account the generally accepted bioethical norms of international regulations regarding the experimental work with vertebrate animals. Blood, tissues: liver, skin and wool were selected for the study. For determination of macro and microelements, the samples were pre-mineralized by dry aching method. After ashes, acid extraction was performed. In the prepared samples, the elements were determined by atomic absorption spectrophotometry using AAS-115 C. The digital data were statistically processed using the Student's t test. Studies have found that the feeding of rabbit sulfur citrate in the smallest amount tested 2 mg/kg caused a significant increase in liver – Fe (P < 0.05) and skin – Zn (P < 0.05), whereas a larger dose of 4 mg/kg was affected by changes in blood Cr and Fe (P < 0.01), liver – Zn and Fe (P < 0.05), skin – Zn (P < 0.01), wool – Zn and Fe (P < 0.05) compared to the control group. It was noted that the use in the diet of rabbits supplements of sulfur citrate at the rate of 8 mg/kg was marked by the greatest changes in the investigated tissues of the rabbit body, in particular the higher blood levels of Cr (P<0,001), Fe (P<0,05) and Cu (P < 0.01), liver – Zn, Fe and Cu (P < 0.05), skin – Zn (P < 0.001), higher amounts of 12 mg/kg caused changes in liver content – Zn (P < 0.01) and Fe (P < 0.05), skin – Zn (P < 0.05) compared to the control, indicating the stimulating effect of the organic sulfur compound on the activation of the processes of assimilation of these elements in their body. The use of sodium sulfate in the amount of 40 mg S/kg body weight was less likely to affect the content of the studied mineral elements compared with the control group with a more pronounced effect: Zn in the liver and skin, Cr in wool. Therefore, feeding in the diet of rabbits II and III experimental groups of nano sulfur citrate in the amount of 4 and 8 S/kg body weight was more pronounced synergistic effect on the trace elements in the tissues of their body than the use of inorganic compounds and the control group.

2018 ◽  
Vol 20 (92) ◽  
pp. 203-208
Author(s):  
A. Z. Dychok-Niedzielska ◽  
Y. V. Lesyk

The article presents results on the influence of different amounts of sulfur citrate, manufactured using nanotechnology and sodium sulfate in rabbit diet from 60 to 118 days of age on the hematological parameters of their organism. Studies have been conducted on 30 rabbits of the Hyla hybrid divided into six groups (control and five experimental), 6 animals in each. Animals were kept in regulated microclimate and illumination in mesh cages of 50×120×30 cm. Controlled rabbits fed without restriction a balanced granulated feed with free access to water. The young of the first (E-II), the second (E-II), the third (E-III) and the fourth (E-IV) experimental groups feed the diet of the control group and, during the day, dispensed sulfur citrate from the calculation of 2; 4; 8 and 12 mg S/kg body weight. Rabbits (E-V) of the experimental group fed the diet of the control group and set water with sodium sulfate (Na2SO4) in the amount of 40 mg S/kg body weight. In the preparatory period – 60 days and in the experimental period – at 91 and 118 days of life (31 and 58 days of delivery of supplements), blood samples were collected from the regional anterior vein of rabbits for hematological studies performed using an automatic hematologic analyzer (Orphee Mythic-18, Switzerland). Researches have shown that the administration of citrate sulfur in the amount of 8 mg S/kg of body weight was marked by a greater number of red blood cells and leukocytes, respectively, by 13.4 and 23.3% (P < 0.05) and with a higher level of HCT at 16.2% (P < 0.05), MCH, RDV and MCHC, respectively, 11.1; 14.5 and 2.6% (P < 0.05) on day 58 of the experiment compared with the control group. In the blood of animals in the 2nd experimental group, a higher concentration of hemoglobin (Р < 0.05) was observed at 31 days and a higher HCT (Р < 0.05) level was 58 days compared to control. Thus, the results of the study of the hematological parameters of the body of rabbits indicate their positive changes in the actions of the physiologically substantiated amount of organic sulfur, which contributed to the activation of metabolic processes.


2021 ◽  
Vol 24 (8) ◽  
pp. 9-14
Author(s):  
Yaroslav Lesyk ◽  
Anna Dychok-Nidzelska ◽  
Oleksandr Boiko ◽  
Mykhailo Bashchenko ◽  
Oleksii Honchar

The use of organic minerals in the form of nanocompounds as a substitute for mineral inorganic salts is promising, but insufficiently studied. Therefore, the purpose of the search was to determine the influence of drinking sulfur citrate and sodium sulfate 14 days before insemination and up to 20th day of lactation on the reproductive capacity of rabbits and the preservation of the offspring up to 40th day of life. The research was carried out on rabbits of the second breed of Hyla breed in “Horlytsia”. Сontrol group were fed without restriction complete ration granular feed with free access to water. 1st experimental group were fed with the ration of the control group and during the day were watered with sulfur citrate, at the rate of 8 μg S/kg of body weight. II experimental group were fed with the ration of the control group and with water was given sodium sulfate in the amount of 40 mg S/kg of body weight. Additives to rabbits were watered 14 days before insemination and for up to 20 days of lactation. It was found that on the first day of life of young rabbits their number in the I and II experimental groups was respectively higher by 8.5 and 4.2% compared to the control. The number of young rabbits at 20 and 40 days of age in the I and II experimental groups was respectively higher by 10.4 and 4.4% and 14.0 and 4.6% compared to the control group. The weight of rabbits in the nest of the II experimental group at 1, 20 and 40 days of life was respectively higher by 2.8; 6.1 and 7.0%, which is based on the average mass of one rabbit for these periods and amounted to 1.1, respectively; 2.7 and 4.3% compared with animals in the control group. The average amount of milk produced by rabbits of I and II experimental groups was respectively higher by 10.2% and 6.6% per day and for 20 days of the lactation period compared to the control. The preservation of rabbits in the I and II experimental groups was respectively higher by 6.4 and 6.4% and 3.6 and 4.4% at 20 and 40 days of life of young rabbits compared to the control group. The results of the research indicate the possibility of additional use in the ration of rabbits of the addition of sulfur citrate in the amount of 8 μg S/kg of body weight to increase metabolism and reproductive capacity during periods of increased physiological load


Author(s):  
S. M. Medvid

The article presents data on the influence of the complex of micro elements (Fe, Cu, Zn, Co, Mn) in the form of aquacitrate on the indices of non-specific and cellular immunity in chicken broilers. The poultry received a fodder, balanced by nutrients, according to its type, age and productivity. Control group of chickens were added standard mineral premix (SP) from inorganic compounds of bioelements. Broilers of experimental groups were given water with a complex mineral additive of aquacitrates. In particular, the chickens of the first experimental group – in the amount corresponding to their content in the standard premix (SP), and the 2nd, 3rd, 4th and 5th experimental groups – in the amount equal to 3/4, 1/2, 1/4 and 1/10 of the content of mineral elements in the standard premix. It was found that bactericidal and lysozyme activity of blood serum (BASB and LASB) were the highest in broiler chickens of the third and fourth experimental groups and exceeded the control values by 5.7 and 5.3%, respectively. In the bird of the second and fifth groups, the studied indicators were closer to the ones in control. However, in the chickens of the group D1, giving them the highest studied dose of aquacitrate of micor elements, the values of BASB and LASB tended to decrease. Concerning the level of CIC in serum, it was the highest in the chickens of the second, third and fourth groups, the lowest in the group D1. A similar pattern is also characteristic for phagocytic activity of pseudoiesinophils (PhA). The bite of half and even the quaternary amount of citrates of micro elements, compared with the amount of bioelements in inorganic form, provided an increase, compared to control, of PhA. At such a dose of micro elements in the nanoform in the blood of broilers, the number of T-total and T-active lymphocytes increased, including T-heeler cells and decreased the relative number of T-suppressors. The bird of the first experimental group was characterized by a decrease in the percentage of T-heeler against the background of a slight increase of T-suppressors. Thus, the results of studies of indices of nonspecific and cellular immunity suggest that micor elements in the nanodisperse form, due to better bioavailability and high bioactivity, have a positive effect on the formation and development of the immune system in broiler chickens at a dose corresponding to 25 and 50%, from recommended quantity of biometals in the standard premix.


2021 ◽  
Vol 42 (4) ◽  
pp. 2471-2488
Author(s):  
Giovanna Bobato Pontarolo ◽  
◽  
Mikael Neumann ◽  
Fernando Braga Cristo ◽  
Edelmir Silvio Stadler Júnior ◽  
...  

Functional additives, such as autolyzed yeasts, have been used to achieve greater production efficiency and animal health. These compounds are also alternatives to the use of performance-enhancing antimicrobials. The objective was to evaluate the productive performance, ingestive behavior, apparent digestibility of feed DM and the carcass characteristics of beef steers finished in feedlot receiving autolyzed yeasts in the diet. The experimental design was randomized blocks, consisting of three treatments and six repetitions, where each repetition was represented by a stall with two animals. 36 bulls, ½ Angus × ½ Nelore blood, from the same herd, with an average age of 11 months and an average body weight of ± 330 kg were used.The treatments were as follows: CON - yeast-free diet; Y4 - yeast diet (4 g animal day-1) and Y7 - yeast diet (7 g animal day-1). The product used is a functional ingredient containing the yeast Saccharomyces cerevisiae. The inclusion of autolyzed yeasts resulted in greater daily body weight gain and better feed conversion in the initial feedlot phase. The apparent digestibility of DM of diets containing autolyzed yeast was superior to the control diet, and its use did not interfere with the animal ingestive behavior. Supplementation with autolyzed yeasts at the inclusion level of 4 g day-1 promoted better results in the finishing of feedlot steers, and that the supplemented groups (4 g animal day-1 and 7 g animal day-1) achieved the same degree of carcass finishing, higher than the control group.


Atmosphere ◽  
2019 ◽  
Vol 10 (12) ◽  
pp. 751 ◽  
Author(s):  
Remigiusz Gałęcki ◽  
Michał Dąbrowski ◽  
Tadeusz Bakuła ◽  
Kazimierz Obremski ◽  
Adriana Nowak ◽  
...  

The aim of this study was to evaluate the influence of the biopreparation Deodoric® on ammonia (NH3) concentration, performance, and hygiene standards in laying hen (ROSS-308) production. Statistically significant differences in NH3 concentration and the body weight of laying hens were observed between the control group (C) and the experimental group (E) where Deodoric® was applied at the set dose. In the control group, an increase in NH3 concentration could have contributed to the decrease in the body weight of laying hens, egg production, and % hen day egg production, whereas no such correlations were observed in the experimental group. A moderate linear correlation between NH3 concentration vs. humidity (r = 0.68), air flow (r = 0.48) and weakly linear correlation between NH3 concentration and age of birds (r = 0.27) was noted in group C. In group E, NH3 concentration vs. temperature (r = 0.27) and humidity (r = 0.14) were weakly correlated. Statistical analysis of changes in the microbial counts isolated from manure revealed a significant decrease of mesophilic microorganisms on day 28 decrease of Campylobacter spp. days 14 and 84 in group E. However, for the entire experimental model no statistically significant changes in the number of Campylobacter spp. and mesophilic bacteria were found. The tested preparation did not cause changes in the microbial composition of tissue swabs. Deodoric® contributes to animal welfare by reducing the ammonia concentrations in poultry houses. It is also recommended for use in poultry farms to improve animal health and performance and to generate benefits for producers.


2004 ◽  
Vol 50 (5) ◽  
pp. 27-32
Author(s):  
I. V. Dvoryashina ◽  
N. T. Starkova ◽  
A. B. Antonov ◽  
Yu. Yu. Monogarova

Hormonal and metabolic disorders underlie the development of coronary heart disease (CHD). Insulin resistance, hyperinsulinemia, dyslipdemia and frequently obesity play a particular role in the pathogenesis of this disease. The purpose of this study was to characterize the basic parameters of the metabolic insulin resistance syndrome in males with CHD and an ideal body weight. A hundred and fifty-eight males were examined. They were divided into 3 groups: 1) patients with CHD and an ideal body weight; 2) those with CHD and obesity; and 3) males without CHD and obesity (a control group). The patients underwent athropometric studies; computed tomography was performed to determine the volume of abdominal fatty tissue; the blood levels of glucose, insulin, and lipids were measured. Dietary food load tests were used to study the functional activity of the lipid-transport system. Group 1 patients were found to have the major signs of the metabolic insulin-resistance syndrome: basal and stimulated hyperinsulinemia, dyslipidemia, the increased volume of visceral fatty tissue, and impaired fat tolerance.


2012 ◽  
Vol 3 (2) ◽  
pp. 100-104 ◽  
Author(s):  
SM Kamruzzaman ◽  
SML Kabir ◽  
MM Rahman ◽  
MW IslaM ◽  
MA Reza

The study was carried out to know the effects of probiotics (Proetxin® Boost, Novartis Bangladesh Ltd.) and antibiotic (oxytetracycline-Renamycin®, Renata Animal Health) on growth performances and haemato-biochemical parameters of "Shaver Star Bro" broiler chickens during September and October 2003. A total of 20, day old broilers were randomly selected and assigned into four equal groups n = 5) as A, B, C and D. Group A was considered as control group fed with commercial ration while groups B, C and D were fed with commercial ration with the addition of 200 mg probiotics / liter drinking water, 100 mg probiotics and 50 mg antibiotic (oxytetracycline- Renamycin®) / liter drinking water and 100 mg antibiotic (oxytetracycline- Renamycin®) / liter drinking water respectively up to 35 days of age. The results showed that the body weight gains differed significantly (p < 0.05) at the 2nd, 4th and 5th weeks of age in different treatment groups. The meat yield not differed significantly (p > 0.05). The drumstick, wing differed significantly (p < 0.01) and spleen weight differed at p < 0.05 among different groups. The mean haemato-biochemical values of Hb, ESR, PCV, heterophil, eosinophil, basophil, triglyceride, HDL, LDL, SGPT and SGOT were differed significantly (p < 0.01) in different groups. The present findings suggest that supplementation of probiotics has significant effect on growth performance and certain haemato-biochemical parameters of broiler chickens as compared to antibiotic supplementation.


Author(s):  
Н. Ю Коропецька ◽  
Д. Д Остапів ◽  
І. О. Нєктєгаєв ◽  
Р. Б Лесик ◽  
О. Р Піняжко

ВПЛИВ РЕТАБОЛІЛУ, РЕЧОВИНИ LES-2222*, ТЕСТОСТЕРОНУ ПРОПІОНАТУ НА МАСУ ТІЛА ТА ВНУТРІШНІХ ОРГАНІВ ЩУРІВ ПРИ ХАРЧОВІЙ ДЕПРИВАЦІЇ - Метою дослідження було вивчення рівня анаболічної дії вказаних речовин на масу тіла і внутрішніх органів щурів за умов харчової депривації. Було сформовано 5 груп тварин по 6 особин у кожній: контрольна І отримувала повноцінний раціон, контрольна ІІ піддавалась харчовій депривації; тваринам дослідних груп вводили щоденно досліджувані речовини. Дослід тривав 9 діб з вільним доступом тварин до води. Статистичну вірогідність визначали за Н. А. Плохін- ським [1]. Результати досліджень показали позитивний вплив застосованих речовин на зміни маси тіла і внутрішніх органів щурів, вірогідність яких підтверджується силою кореляційного відношення між величиною показників щурів контрольних і дослідних груп до та після проведення експерименту, яка зростала від слабкого (т|2=0,115) до сильного (т|2=0,718) для маси тіла, т|2=0,554 для печінки, п2=0,377 для нирки, п2=0,308 для сім’яників; для маси селезінки і серця сила кореляційного відношення не перевищувалат|2=0,209. При цьому, найменше зниження маси тіла тварин виявлено при введенні речовини LES-2222-14,8 %, проти 19,3 та 20,3 % за дії ретаболілу і тестостерону пропіонату, маси печінки -10,1; 8,7 та 4,3 %, нирки - 4,6; 13,7 і 18,2 %, сім’яника - 7,9; 9,9 та 11,8 % відповідно. Таким чином, введення досліджуваних речовин в організм щурів, які піддавались харчовій депривації, призводить до вірогідного зменшення величини показника зниження маси тіла та внутрішніх органів тварин. Максимально високу анаболічну активність забезпечує речовина LES-2222. Ймовірно, досліджувані засоби гальмують процеси катаболізму, що проявляється меншими втратами як маси тіла тварин в цілому, так і внутрішніх органів зокрема.<br />ВЛИЯНИЕ РЕТАБОЛИЛА, ВЕЩЕСТВА LES-2222 *, ТЕСТОСТЕРОНА ПРОПИОНАТА НА МАССУ ТЕЛА И ВНУТРЕННИХ ОРГАНОВ КРЫС ПРИ ПИЩЕВОЙ ДЕПРИВАЦИИ - Целью исследования было изучение уровня анаболического действия указанных веществ на массу тела и внутренних органов крыс при пищевой депривации. Было сформировано 5 групп животных по 6 особей в каждой: контрольная I получала полноценный рацион, контрольная II подвергалась пищевой депривации; животным исследовательских групп вводили ежедневно исследуемые вещества. Опыт длился 9 дней со свободным доступом животных к воде. Статистическую вероятность определяли по Н. А. Плохинс- кому [1]. Результаты исследований показали положительное влияние применяемых веществ на изменения массы тела и внутренних органов крыс, вероятность которых подтверждается силой корреляционного отношения между величиной показателей крыс контрольных и опытных групп до и после проведения эксперимента, которая росла от слабого (п2=0,115) до сильного (п2=0,718) для массы тела,п2=0,554 для печени, п2=0,377 для почки, п2=0,308 для семенников; для массы селезенки и сердца сила корреляционного отношения не превышалап2=0,209. При этом наименьшее снижение массы тела животных обнаружено при введении вещества LES-2222-14,8 %, против 19,3 и 20,3 % за действия рета- болила и тестостерона пропионата, массы печени-10,1; 8,7 и 4,3 %, почки 4,6; 13,7 и 18,2 %, семенника - 7,9; 9,9 и 11,8 % соответственно. Таким образом, введение исследуемых веществ в организм крыс, подвергавшихся пищевой депривации, приводит к достоверному уменьшению величины показателя снижения массы тела и внутренних органов животных. Максимально высокую анаболическую активность обеспечивает вещество LES-2222. Вероятно, исследуемые вещества тормозят процессы катаболизма, что проявляется меньшими потерями как массы тела животных в целом, так и внутренних органов в частности.<br />EFFECT OF RETABOLIL, LES-2222* SUBSTANCE, TESTOSTERONE PROPIONATE ON BODY WEIGHT AND INTERNAL ORGANS<br />OF RATS UNDER FOOD DEPRIVATION - The aim of study was to investigate the anabolic action of these substances on body weight and internal organs of rats under conditions of food deprivation. It was formed 5 groups of animals, 6 heads in each: I control one received a full-diet, II control group-subjected to food deprivation; animals were injected daily the research substances. The experiment lasted for 9 days with free access of animals to water. Statistical significance was determined by N. A. Plohinskyi [1]. The results showed a positive effect of applied substances on weight change and internal organs of rats, the probability of which is confirmed by the strength of the correlation ratio between the size indices rat control and experimental groups before and after the experiment, which grew from weak (n2 = 0.115) to severe (n2 = 0.718) for body weight, n2 = 0.554 for the liver, n2 = 0.377 for the kidneys, n2 =<br />0.308 for the testes; for the mass of the spleen and heart strength of the correlation ratio did not exceed n2 = 0.209. The least weight loss was observed when administered to animals the LES-2222 substance - 14.8 %, against 19.3 and 20.3 % for the actions of retabolil and testosterone propionate, liver weight, 10.1; 8.7 and 4.3 %, kidney-4.6; 13.7 and 18.2 %, testis-7.9; 9.9 and 11.8%, respectively. Thus, the introduction of these substances in the body of rats under food deprivation, leads to a possible decrease in the index of weight loss and internal organs of animals. Maximum high anabolic activity provides LES- 2222 substance. Probably the research substances inhibit catabolic processes, shown smaller loosses in body weight of animals in general and in internal organs in particular.<br />Ключові слова: анаболічна дія, харчова депривація, ретаболіл, тестостерону пропіонат, речовина LES-2222.<br />Ключевые слова: анаболическое действие, пищевая депривация, ретаболил, тестостерона пропионат, вещество LES-2222.<br />Key words: anabolic action, food deprivation, retabolil, testosterone propionate, LES-2222 substance.


2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Eugeniusz R. Grela ◽  
D. Gugała ◽  
Marian Flis

AbstractThe aim of the study was to determine the effect of these substitutions on body weight and slaughtering performance, post-mortem changes, sensory characteristics and chemical composition of selected muscle groups of male and female pheasants. The tests were carried out on pheasants during 5–16 weeks of rearing in enclosed aviaries. Four groups of 8 females and 8 males were formed, and the experimental factor was the supplementation of calcium, iron, zinc and copper salts with glycinates of these elements. The control group received a feed blend containing 100% of the mineral need as salts. In the E-25 group, 25% of the salts were replaced by glycinates, in the E-50 group 50% of the salts were replaced, and in the E-75 group only glycinates comprising 75% of the total mineral content were used and no salts were included, with vitamin D3 replaced by calcitriol. For the duration of the study, feeding was at will and the birds had free access to water. In addition, pheasants were weighed at the age of 4, 8, 12 and 16 weeks. Daily feed intake was monitored. At the end of the experiment, when all the pheasants were 16 weeks old, they were weighed and decapitated. The breast, thighs and drumsticks and the heart, liver and gizzard were removed from the chilled carcasses. Supplementation of calcium, iron, zinc and copper salts with 50% glycinates in feed mixtures for pheasants over a period of 5–16 weeks resulted in a slight improvement in the slaughter weight of birds and a significant increase in crude ash, calcium and zinc, while at 75% supplementation and when calcitriol was used instead of vitamin D3, increased phosphorus levels were found in the pheasant muscles analyzed. Males had higher body weight, body weight gain, carcass, liver and gizzard weights, and their muscles had more magnesium and potassium and less calcium, sodium and iron than females.


2019 ◽  
Author(s):  
M. N. Hasan ◽  
M. S. Jahan ◽  
M. S. Kamal ◽  
M. F. Islam ◽  
S. Ahmed ◽  
...  

Background: Vitamin and mineral supplementation is common practice in both human and animal health, But indiscriminate, overdose and long term used may cause toxicity. The research work was designed to evaluate the effect of iron (Fe) &vitamin B12 (Vit-B12) on body weight, hematological parameters: Total Erythrocyte Count (TEC), Hemoglobin (Hb) and Total Leukocyte Count (TLC), and biochemical parameters: serum creatinine (SC), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels in mice. Methods: One-month old 20 female Swiss Albino mice (Rattus norvegicus) having 19.52±0.54 g body weight were divided into four equal groups (n=5). After acclimatization, the mice of group A was treated with Fe@ 0.2 mg/kg body weight (bwt) in pellet, group B was treated with Vit-B12 @ 5 mg/kg bwt, group C was treated with combination of Fe and Vit-B12 and group D was fed with standard pellet and was considered as control. Results: The result showed significant (P<0.05) increase in body weight of treatment groups compared to the control group. The values of TEC, Hb and TLC in the treated groups were increased significantly (P<0.05). ALT, AST and SC values were decreased significantly (P<0.05) in the treated groups compared to the control group. Conclusions: It is concluded that administration of Fe and Vit-B12 significantly (P<0.05) increased body weight and hematological (TEC, Hb, and TLC) parameters however biochemical parameters (ALT and AST) were decreased significantly (P<0.05). In addition, combination of Fe&Vit-B12 showed better effects compared to their single uses.


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