Uji Efektivitas Sediaan Sirup Antipiretik Ekstrak Flavonoid Daun Alvokad (Persea Americana Mill) pada Tikus Putih (Rattus Novergicus L) yang Diinduksi Vaksin DPT-HB.

This study aims to determine the most effective dose in reducing fever in white rats induced by DPT-HB vaccine. The research method is an experimental laboratory using the method without heating for the manufacture of syrup. The results of initial temperature measurements in all treatment groups of white rats which is the normal temperature of the test animals before the DPT-HB vaccine is obtained ranged between 35.10C-37.10C. after 2 hours of DPT-HB vaccine injection, the body temperature of the white rats was measured again and the results obtained were 36,930C-38,430C. an increase in body temperature after 2 hours of injecting this vaccine compared to the results of measuring body temperature before the vaccine is injected is a sign that the test animal has experienced a fever. The negative control group which was given syrup without extract orally, showed the body temperature of the test animals had decreased as the temperature after injecting the vaccine. On the administration of alvocate leaf flavonoid syrup, the dose ¼ showed a decrease of 0.40C. For the administration of dosage flavonoid syrup extract dosage 1, it showed that the body temperature of the test animals had a temperature drop of 1,330C. And administration of Alvocado leaf flavonoid syrup extract dose 2, showed that the body temperature of the test animals had a temperature drop of 0.6 . While the administration of Alvocado leaf flavonoid extract syrup dose 3, showed that the body temperature of the test animals experienced a temperature increase of -0.2 . From the results obtained, it can be concluded that the most effective dose of antipyretic syrup of alvocado leaf flavonoid extract is dose 1.

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UJI AKTIVITAS ANTIPIRETIK EKSTRAK DAUN SISIK NAGA(Pyrrosia piloselloides (L.) M.G. Price) TERHADAP TIKUS PUTIH (Rattus norvegicus) JANTAN GALUR WISTAR YANG DIINDUKSI PEPTON 5%

JIFFK : Jurnal Ilmu Farmasi dan Farmasi Klinik ◽

10.31942/jiffk.v15i01.2169 ◽

2018 ◽

Vol 15 (01) ◽

pp. 22

Author(s):

Ratna Widyasari ◽

Dina Yuspitasari ◽

Fadli Fadli ◽

Athiah Masykuroh ◽

Winda Tahuhiddah

Keyword(s):

Body Temperature ◽

Rattus Norvegicus ◽

Test Group ◽

Negative Control ◽

The Body ◽

Control Group ◽

Antipyretic Activity ◽

White Rats ◽

Infra Red ◽

Pyrrosia Piloselloides

INTISARI Demam adalah keadaan ketika suhu tubuh meningkat melebihi suhu tubuh normal. Demam terjadi karena pelepasan pirogen dari dalam leukosit yang sebelumnya telah terangsang oleh pirogen eksogen yang dapat berasal dari mikroorganisme atau merupakan suatu hasil reaksi imunologik yang tidak berdasarkan suatu infeksi. Sementara itu, banyak negara telah mengembangkan pengobatan menggunakan herbal. Salah satu usaha yang dilakukan adalah dengan penggunaan obat tradisional. Penelitian ini bertujuan untuk membuktikan bahwa daun Sisik Naga (Pyrrosia piloselloides (L.) M.G Price) dengan konsentrasi 0,062%, 0,125% dan 0,25% memiliki aktivitas antipiretik dengan menggunakan penginduksi demam Pepton 5%. Penelitian ini merupakan penelitian eksperimental dengan Rancang Acak Lengkap (RAL). Pengukuran suhu rektal tikus menggunakan termometer infra red. Hewan uji dibagi menjadi 5 kelompok yaitu kelompok kontrol negatif (suspensi Na-CMC 1%), kelompok kontrol positif (suspensi parasetamol) dan kelompok uji yaitu pemberian ekstrak daun sisik naga 0,062%, 0,125% dan 0,25%. Data yang diperoleh dianalisis dengan menggunakan uji anova dan uji LSD (Least Significant Different). Hasil dari penelitian ini bahwa ekstrak daun sisik naga (Pyrrosia piloselloides (L.) M.G Price) konsentrasi 0,25% mempunyai aktivitas antipiretik pada tikus putih (Rattus norvegicus) jantan galur wistar. Kata kunci: Antipiretik, Daun Sisik Naga (Pyrrosia piloselloides (L.) M.G Price), Pepton 5% ABSTRACT A fever is a condition when the body temperature rises beyond the normal body temperature. Fever occurs due to the release of pyrogytes from within leukocytes that have previously been stimulated by exogenous pyrogens that may originate from microorganisms or are a result of immunologic reactions that are not based on an infection. Meanwhile, many countries have developed treatments using herbs. One of the efforts done is with the use of traditional medicine. This study aims to prove that the leaves of the Dragon Scales (Pyrrosia piloselloides (L.) M.G Price) with concentrations of 0.062%, 0.125% and 0.25% have antipyretic activity using Pepton fever induction 5%. This research is an experimental research with Completely Random Design (RAL). Rectal temperature measurements of mice using infra red thermometer. Test animals were divided into 5 groups: negative control group (1% Na-CMC suspension), positive control group (paracetamol suspension) and the test group were 0.062%, 0.125% and 0.25%. The data obtained were analyzed using anova test and LSD (Least Significant Different) test. The results of this study showed that the extract of leaf scales (Pyrrosia piloselloides (L.) M.G Price) concentrations of 0.25% had antipyretic activity in white rats (Rattus norvegicus) male wistar strain. Keywords: Antipyretics, Leaf Scales Dragon (Pyrrosia piloselloides (L.) M.G Price), Pepton 5%

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Protection Effectiveness Of Bit (Beta vulgaris) Fruit Extract In Maintaining Spermatogenesis Ability In White Rats (Rattus novergicus) Induced Carbon Tetracloride (CCl4)

Journal of Basic Medical Veterinary ◽

10.20473/jbmv.v10i2.31812 ◽

2021 ◽

Vol 10 (2) ◽

pp. 36

Author(s):

Alvyan Lantang Anugrah ◽

Hana Eliyani ◽

Budi Utomo ◽

Suherni Susilowati ◽

Maslichah Mafruchati ◽

...

Keyword(s):

Beta Vulgaris ◽

Sertoli Cells ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Male Rats ◽

Control Group ◽

Root Extract ◽

Beet Root ◽

White Rats

The aim of this research was to know whether beetroot (Beta Vulgaris) extract could protect spermatogenesis by maintaining spermatogenic and sertoli cell count in rats (Rattus novergicus) induced with CCl4. Rats were given beetroot extract daily, for 14 days and 3ml/kg BW CCl4 intraperitoneally one hour after last treatment. This study used twenty rats which were devided equally into 5 groups. K(-), the negative control group was not induced with CCL4 and only given 1% CMC-Na suspension. K(+), the positive control group was induced with CCl4 and given 1% CMC-Na suspension. P1, P2 and P3 were given beet root extract with doses of 200, 400, and 800 mg/kg BW daily before feeding. All of the beetroot treatment were given orally (2 ml). After 24 hours CCl4 induction, rats were sacrificed and testis were collected to make histology slides. The observations showed significantly different (p<0,05) in all of variables. Result showed significant differences in spermatogenic and sertoli cells between K(-) and K(+) groups, K(+) with P2 and P3 group, and showed insignificant difference between P2 and P3 group in spermatogenic and sertoli cells. The result of this research showed that beetroot extract could protect the spermatogenic and sertoli cells in male rats induced with CCl4.

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The Effect of Ethanol Extract of Guava Leaf on Hypercholesterolemia-Diabetes of White Rats Male Induced by High Fat Feed and Streptozotocin

Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) ◽

10.22487/j24428744.2021.v7.i2.15533 ◽

2021 ◽

Vol 7 (2) ◽

pp. 177-187

Author(s):

Joni Tandi ◽

Franciska Diana Pratiwi ◽

Surisna Surisna ◽

Niluh Puspita Dewi ◽

Magfirah Magfirah

Keyword(s):

Blood Glucose ◽

Effective Dose ◽

Ethanol Extract ◽

Negative Control ◽

Control Group ◽

Glucose Levels ◽

White Rats ◽

Blood Glucose Levels ◽

Group I ◽

Guava Leaf

This study aims to determine the effect of guava leaf extract (Psidiium guajava L) on hypercholesterolemia-diabetes and the effective dose for reducing cholesterol and blood glucose levels. This study is a laboratory experimental study using 30 rats divided into 6 test animals. the treatment group, each group consisted of 5 rats. Group I was normal control, group II negative control was given Na-CMC suspension, group III positive control was given simvastatin group IV dose 150 mg / kg BW, group V dose 250 mg / kg BW and group VI dose 350 mg / kg BW. The results showed that the ethanol extract of guava leaves had an effect on reducing blood glucose levels of male white rats with an effective dose of 250mg / kg BW with an average reduction of 119 mg/dL and effectively reduce cholesterol levels at a dose of 150 mg /kg BW with an average reduction of 28,33 mg/dL.

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HISTOPATHOLOGICAL STRUCTURE OF WHITE RATS LIVER AFTER GIVING ANT NEST EXTRACT DUE TO INDUCED PARACETAMOL TOXIC DOSE

Buletin Veteriner Udayana ◽

10.24843/bulvet.2019.v11.i01.p03 ◽

2019 ◽

pp. 14

Author(s):

I Made Merdana ◽

I Made Kardena ◽

Ketut Budiasa ◽

I Made Dodi Gunawan

Keyword(s):

Rat Liver ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Toxic Dose ◽

White Rats ◽

Significant Difference ◽

Ant Nest ◽

Histopathologic Changes

This study aim was to determine the influence ant nest plant extract (Myrmecodia pendans) on histopathological changeof white rat liver (Rattus novergicus) due to induced with paracetamol toxic dose. This study used 24 male white rats, divided into four groups, negative control group (P0) given placebo, positive control group (P1) given paracetamol dose 250 mg / kg bw for 10 days, P2 given ant nest extract 250 mg / kg bw and paracetamol dose 250 mg / kg bw for 10 days, P3 given ants nest extract 250 mg / kg bw for seven days, then continued by giving paracetamol and ants nest extract with dose 250 mg / kg bw for ten days. After the treatment done, all the rats were dinecropsed. Liver organs were taken and processed for making histopathology preparations. Parameters examined included hemorrhage, congestion, degeneration and necrosis. The data obtained were analyzed statistically by using Kruskal Wallis test followed by Mann Whitney test. Mann Whitney test results for all categories of histopathologic changes in hemorrhagic, congestion, degeneration, and necrosis between negative control group (P0) and positive control group (P1) were significantly different (P <0.05), between negative control (P0) with P2 and P3 there was no significant difference (P> 0,05). Afterward, between the positive control (P1) and P2 with P3 there was a significant difference (P <0.05). I can be concludedthat the administration of paracetamol dose 250 mg/kg bw for 10 days affects the histopathologic changes of white rat liver. The administration of ant nest plant extracts can reduce the side effects of toxic doses of paracetamol.

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Pengaruh Kitosan Terhadap Jumlah Osteoklas dan Osteoblas Pada Tikus Galur Wistar Model Menopause

Journal of Islamic Medicine ◽

10.18860/jim.v1i2.4456 ◽

2017 ◽

Vol 1 (2) ◽

pp. 76-87

Author(s):

Muhammad Nur

Keyword(s):

Treatment Group ◽

Total Sample ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Positive Control Group ◽

Control Group ◽

Control Group Design ◽

White Rats ◽

Significant Difference

Chitosan is one of the most abundant and dispersed polymers in nature. It has a rapid healing effect for tissues and has an effect on bone remodeling. The purpose of this study was to prove that giving chitosan influenced the decrease of osteoclast number and increasing number of osteoblasts in female mice of wistar strain of ovariectomy menopause model.This study used a posttest only control group design. The total sample of 30 rats divided into three groups was randomized, osteoporosis was made with the menopausal model which was performed ovariectomy in the positive control group and the treatment group then in the treatment group was given chitosan extract of single dose of 45 mg / day via Nasogastric Tube (NGT). K1 was a negative control group (without ovariectomy and without chitosan), K2 was a positive control group (with no chitosan ovariectomy), K3 was the treatment group (ovariectomy and chitosan 45 mg / day).The results showed significant differences in the number of osteoblasts between K1 and K3, K2 with K3, and no significant differences were found in the K1 group with K2. While the number of osteoclasts showed that there were significant differences in K1 group with K2 and no significant difference was found in K1 group with K3, K2 with K3.Conclusions in this study showed that 45 mg / day of chitosan doses could significantly increase osteoblast counts in menopause female white rats and decrease the number of osteoclasts performed by ovariectomy with chitosan administration.

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Efficacy of Topical Coffee Paste to Collagen Density in Wistar Rats Incision Wound

BioScientia Medicina ◽

10.32539/bsm.v2i1.14 ◽

2018 ◽

Vol 2 (1) ◽

pp. 12-15

Author(s):

Debby Handayati Harahap ◽

Fifa Argentina ◽

Yuli Kurniawati ◽

Citra Tresna Murti ◽

Yudha Dwi Satrio

Keyword(s):

Vitamin C ◽

Design Research ◽

Negative Control Group ◽

Negative Control ◽

T Test ◽

Control Group ◽

Average Difference ◽

White Rats ◽

Collagen Density ◽

Animal House

Background :Collagen plays a very important role in humans at every stage of wound healing. Coffee contains many antioxidants that stimulate the formation of collagen dermis by increasing the production of Tissue Inhibitor of Matrix Metalloproteinas-1 in the dermis that serves to inhibit the breakdown of collagen. This study aims to determine the efficacy of topical pasta coffee to collagen density. Methods: Experimental Laboratories Study with pre and post test controlled group design research was undertaken from September to November 2017 at Animal House and Dyatnitalis Laboratory, Palembang. 30 male white rats of wistar strain were divided into 5 groups then performed 2 cm dermal incision and giving various concentration of coffee paste. On the day 0 and 5th after coffee paste, the percentage of collagen density is checked. The efficacy of coffee paste was analyzed by Paired T Test/Wilcoxon while the comparison of efficacy was analyzed by Independent T Test / Mann Whitney then continued with One Way ANOVA Test and Tukey test. Data analysis using SPSS version 18.0. Results: There was an average difference of percentage level of collagen density before and after vitamin C (p = 0,003), coffee paste 20% (p = 0,043), coffee paste 40% (p = 0,045) and coffee paste 80% (p = 0,011) . In addition, there was an average difference of percentage of collagen density between negative control group with positive control group and 40% coffee paste. From the results of Post Hoc test results showed that there was a difference of percentage of collagen density of negative control group with coffee paste group 40%. Conclusion: There is efficacy of topical coffee paste on collagen density in various concentrations where the highest increase of collagen density is 246,96% after coffee paste 40%. Keyword: Coffee Pasta, Collagen, Injured Incision, Vitamin C

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Percepatan Penutupan Luka Sayat pada Tikus Putih Akibat Pemberian Perasan Rimpang Temulawak (Curcuma xanthorrhiza Roxb.)

Jurnal Pharmascience ◽

10.20527/jps.v8i1.9333 ◽

2021 ◽

Vol 8 (1) ◽

pp. 1

Author(s):

Elisabeth Natalia Barung ◽

Rifny Wungow ◽

Donald Emilio Kalonio

Keyword(s):

Wound Healing ◽

Linear Regression Analysis ◽

Negative Control Group ◽

Negative Control ◽

Control Group ◽

Treatment Groups ◽

Accelerate Wound Healing ◽

White Rats ◽

Curcuma Xanthorrhiza ◽

Incision Wound

Rimpang Temulawak atau Curcuma xanthorriza Roxb. adalah tanaman yang dikenal luas oleh masyarakat sebagai obat tradisional. Rimpang temulawak mengandung kurkumin dan xanthorrizzol, yang diketahui mampu mempercepat penutupan luka di kulit dan juga memiliki efek antibakteri dan antiinflamasi. Penelitian ini bertujuan untuk untuk mengetahui efektifitas perasan rimpang temulawak terhadap percepatan penutupan luka sayat pada tikus putih. Penelitian ini adalah penelitian eksperimen, dengan subyek 10 ekor tikus putih yang dibagi dalam 2 kelompok perlakuan yaitu kelompok yang diberi perasan temulawak dan kelompok kontrol negatif yang tidak diberi perlakuan. Data dikumpulkan dengan mengukur panjang luka tikus menggunakan alat ukur penggaris dan dihitung persentasi penutupan luka. Data dianalisis menggunakan analisis regresi linear dan nilai slope (b) dinyatakan sebagai kecepatan penutupan luka. Hasil penelitian ini, menunjukan bahwa rimpang temulawak mampu mempercepat penutupan luka sayat sebesar 15,262%/hari dibandingkan kelompok yang tidak diberi perlakuan sebesar 13,54%/hari. Kata Kunci: Perasan Rimpang Temulawak, Luka Sayat, Percepatan Penutupan Luka, Sediaan Sederhana, Obat Tradisional Indonesia Curcuma xanthorrhiza Roxb. is a plant that is widely known by the community as traditional medicine. The rhizome of C. xanthorrhiza contains curcumin and xanthorrhizol, which are known to be able to accelerate wound healing on the skin and also has antibacterial and anti-inflammatory effects. This study was aimed to determine the effectiveness of C. xanthorriza rhizome on the acceleration of incision wound healing on white rats. This study was an experimental study, with 10 white rat subjects divided into two treatment groups, namely the treatment group which was given by C. xanthorriza rhizome and the negative control group that was not treated. Data were collected by measuring rat wound length using a ruler and calculating the percentage of wound healing. By using linear regression analysis and the value of the slope (b) is expressed as the acceleration of wound healing. The results of the study showed that C. xanthorrhiza rhizome was able to accelerate incision wound healing by 15.262% / day compared to the untreated group of 13.54% / day.

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Ameliorative Effect of Ethanolic Extract of Petroselinum Crispum Against Gentamicin-Induced Nephrotoxicity in Rabbits Male

Al-Anbar Journal of Veterinary Sciences ◽

10.37940/ajvs.2020.13.2.5 ◽

2020 ◽

Vol 13 (2) ◽

Author(s):

Sarah Hussain ◽

Mariam Kadhem

Keyword(s):

Body Weight ◽

Clinical Signs ◽

Ethanolic Extract ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

The Body ◽

Positive Control Group ◽

Control Group ◽

Petroselinum Crispum

The experiment was conducted to investigate the protective effect of Petroselinum crispum leave extracted against gentamicin-induced nephrotoxicity in male rabbits by studying the body weight, clinical signs, haematological and biochemical parameters, gross lesion and histopathological changes. Twenty four rabbits male were used and divided into 4 groups. Group 1: rabbits served as a negative control, received distilled water 1 ml(orally). Group 2: rabbits served as a positive control group, received gentamicin at a dose of 80 mg/kg/day intramuscular for 15 days. Group 3: rabbits received gentamicin at a dose of 80 mg/kg/day then after one hour treated with ethanolic extract of Petroselinum crispum at dose 125 mg/kg orally for 15 days. Group 4: rabbits received gentamicin at a dose of 80 mg/kg/day then after one hour treated with ethanolic extract of Petroselinum crispum at dose 250 mg/kg orally for 15 days.The results of the gentamicin treated group( positive control group) showed clinical signs such as loss of body weight, loss of appetite and rough hair with hematuria. The body weight a significantly declined (p≤ 0.05) compared other groups. There was a significant decrease (p≤ 0.05) in WBC count, lymphocyte, GSH, SOD, CAT, and GPX levels, while it recorded a significant increase (p≤0.05) in weights of the kidneys, neutrophils, creatinine, urea, and MDA. Histological studies showed several kidney pathological changes such as pale colour, enlargement in size and weight and easy from detaching as opposed to negative control group. On the other hand, the group treated with ethanolic extractof Petroselinum crispum at dose 125 mg/kg induced improved of parameters as recorded significant increased(P ≤ 0.05) in body weight, WBC count, lymphocyte, GSH, SOD, CAT, and GPX, while significant decreased (P ≤ 0.05) in weights of the kidneys, neutrophils, creatinine, urea, and MDA compared with the positive control group whereas rabbits treated with ethanolic extract of Petroselinum crispum at dose 250 mg/kg restored the parameters and histological changes of the kidney to near normal status compared with the negative control group. These results showed that the dose-detected Petroselinum crispum extract (250mg / kg) acts as potential curative effect against gentamicin-induced nephrotoxicity in male rabbits.

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UJI EFEK ANTIPIRETIK EKSTRAK MENIRAN (Phyllantus niruri L.) PADA TIKUS WISTAR (Rattus norvegicus) JANTAN YANG DIINDUKSI VAKSIN DPT-HB

Jurnal e-Biomedik ◽

10.35790/ebm.3.1.2015.7427 ◽

2015 ◽

Vol 3 (1) ◽

Author(s):

Ivana Jansen ◽

Jane Wuisan ◽

Henoch Awaloei

Keyword(s):

Wistar Rats ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Distilled Water ◽

Phyllanthus Niruri ◽

Antipyretic Effect ◽

Flower Extract ◽

Hb Vaccine

Abstract: The aim of this study is to investigate the antipyretic effect of leaf-flower extract (Phyllanthus niruri L.) in Wistar rats induced by DPT-HB vaccine. Samples were 15 Wistar rats as test animals and were divided into five groups of three in each. The negative control group was given orally distilled water, positive control group was given paracetamol and 3 experimental groups were given ethanolic leaf-flower extracts (100 mg, 200 mg and 300 mg /200 g body weight of rats). Pyrexia was induced in rats by intramuscular injection of 0.2 ml DPT-HB vaccine. Antipyretic activity was measured at 0, 30, 60, 90, 120, 150, 180 minutes after administration of leaf-flower extract, paracetamol and distilled water. The results showed that leaf-flower extract at a dose of 300 mg /200 g BW decreased the rectal temperature greater than with doses of 100 and 200 mg /200 g BW for 180 minutes of measurement. Conclusion: Leaf-flower extract has antipyretic effect in Wistar rats.Keywords: antipyretic, phyllanthus niruri, DPT-HB, wistar ratAbstrak: Penelitian ini bertujuan untuk menguji efek antipiretik ekstrak meniran (Phyllanthus niruri L.) pada tikus Wistar yang diinduksi vaksin DPT-HB. Metode: Hewan uji yang digunakan adalah 15 ekor tikus Wistar yang dibagi ke dalam 5 kelompok, masing-masing kelompok terdiri dari 3 ekor tikus. Kelompok kontrol negatif diberikan aquades, kelompok kontrol positif diberikan parasetamol, dan 3 kelompok eksperimental diberikan ekstrak tanaman meniran masing-masing dengan dosis 100mg, 200mg dan 300 mg/200 grBB tikus. Induksi demam pada hewan uji menggunakan vaksin DPT-HB 0,2ml secara intramuskular. Pengukuran suhu rektal dilakukan sebelum dan sesudah pemberian vaksin dan setelah pemberian bahan uji yaitu pada menit ke-30, 60, 90, 120, 150 dan 180. Hasil penelitian memperlihatkan pemberian ekstrak meniran dengan dosis 300 mg/200 grBB menunjukkan penurunan suhu rektal lebih besar dibanding dengan dosis 100 dan 200 mg/200 grBB selama 180 menit pengukuran. Simpulan: Ekstrak meniran memiliki efek antipiretik pada tikus Wistar.Kata kunci: antipiretik, phyllanthus niruri, DPT-HB, tikus wistar

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The bone strengthening effects of propolis in ovariectomized female white rats as models for postmenopause

Current Issues in Pharmacy and Medical Sciences ◽

10.2478/cipms-2021-0025 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Dian Ayu Juwita ◽

Almahdy Almahdy ◽

Rahmad Abdillah ◽

Fiony Syahputri

Keyword(s):

Bone Strength ◽

Testing Machine ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Impact Testing ◽

Control Group ◽

Test Results ◽

Treatment Groups ◽

White Rats

Abstract Osteoporosis is a bone disease characterized by decreased quality and strength of bones so that it becomes porous and fracture. Propolis is known to have many pharmacological activity, including an anti-osteoporosis effect. This study aims to determine the effect of propolis administration and the effects of propolis dosage variation in preventing osteoporosis based on the strength value of femur bone impact in female white rats in the form of an ovariectomy postmenopausal model. The rats were divided into 5 groups: positive control group (subjected to ovariectomy), negative control group (not subjected to ovariectomy, and treatment groups that were subjected to ovariectomy and given propolis at a dose of 180 mg/kg BW, dose 360 mg/kg BW and dose 720 mg/kg BW. Propolis was administered orally for 30 days. Bone impact strength testing was undertaken after 30 days using an impact testing machine. Research data were analyzed via one-way ANOVA and continued with the Duncan’s Multiple Range Test. From the test results, we noted that propolis administration had an effect on the value of bone strength, with the dose of 720 mg/kg BW and 360 mg/kg BW having a significant effect, compared with others. With an increase in dose, propolis can provide an increase in the value of bone strength in rat bones.

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