scholarly journals Physiological alterations and enzymatic evaluation of soybean cultivars under water deficit

2021 ◽  
Vol 7 ◽  
pp. 1-10
Author(s):  
Wellington Gomes ◽  
Samy Pimenta ◽  
Larissa Souza Amaral ◽  
Bruno Rafael Alves Rodrigues ◽  
Aluizio Borem
Keyword(s):  
Lipid Peroxidation ◽  
Water Deficit ◽  
Abiotic Factors ◽  
Development Stage ◽  
Soybean Cultivars ◽  
Water Potentials ◽  
Physiological Alterations ◽  
Activity Of Enzymes ◽  
Biochemical Mechanisms ◽  
Increased Activity

Drought is one of the main abiotic factors limiting agricultural productivity, capable of having a major impact on the yield of most crops. The knowledge of the physiological and biochemical mechanisms that differentiate resistance and susceptibility to water deficit among soybean strains can be used in the generation of more tolerant cultivars. In this sense, the objective of this study was to characterize physiologically, two soybean cultivars with different patterns of tolerance to drought in the field, by determining photosynthetic rates, lipid peroxidation levels and antioxidant enzyme activity under three levels of water potential. Upon reaching the V4 development stage, the irrigation of the plants was suspended and three data collection were performed: full irrigation (control); moderate water deficit (Ψ = -1.5 ± 0.2 MPa) and severe deficit (Ψ = -3.0 MPa ± 0.2 MPa). Variations in perspiration rate, stomatal conductance, as well as decrease in photosynthetic rate were significant between the two cultivars, where the water potentials in cultivar BR 16 anticipated on average two days achieving the same water potentials in Embrapa 48 cultivar, thus presenting better efficiency in water use. In addition, the increased activity of enzymes and lipid peroxidation were more significant in the cultivar BR 16, demonstrating that this cultivar is less tolerant to drought than Embrapa 48 cultivar, corroborating to agronomic data previously found in the field.

scholarly journals CHLORIMURON-ETHYL IN CONVENTIONAL AND TRANSGENIC SOYBEAN CULTIVARS UNDER WATER DEFICIT STRESS

2018 ◽  
Vol 31 (4) ◽  
pp. 832-842
Author(s):  
Clebson Gomes Gonçalves ◽  
Antonio Carlos da Silva Junior ◽  
Maynumi Scarano ◽  
Maria Renata Rocha Pereira ◽  
Dagoberto Martins
Keyword(s):  
Root System ◽  
Soil Water ◽  
Water Deficit ◽  
Dry Weight ◽  
Water Deficit Stress ◽  
Limiting Factor ◽  
Soybean Cultivars ◽  
Water Potentials ◽  
Chlorimuron Ethyl ◽  
Soybean Plants

ABSTRACT Water deficit is a limiting factor for the soybean yield; it triggers different physiological and anatomical adaptations that have deleterious effects on the plants and can affect the selectivity of herbicides, causing production losses. In this context, the objective of this work was to evaluate the action of the chlorimuron-ethyl herbicide when applied at different stages of soybean plants, using conventional and transgenic cultivars, and different soil water potentials. A rate of 20 g ha-1 of the chlorimuron-ethyl herbicide was applied to two soybean cultivars (MG/BR46-Conquista - conventional, and BRS-Valiosa-RR - transgenic) at two phenological stages (V2 - first fully expanded trifoliate leaves, and V4 - third fully expanded trifoliate leaves), using three soil water potentials (-0.03 MPa, -0.07 MPa, and -0.5 MPa). Phytotoxicity, and plant height were evaluated at 3, 7, 14, and 21 days after the herbicide application. The shoot dry weight, root dry weight, and root system nodulation were evaluated. The soybean plants had lower phytotoxicity when subjected to application of chlorimuron-ethyl under water deficit conditions. The use of chlorimuron-ethyl reduced the growth and biomass of soybean plants and affected the plants' root system nodulation. The transgenic cultivar (BRS-Valiosa-RR) presented better performance when subjected to a moderate water deficit (-0.07 MPa), which contributes to biological nitrogen fixation.

Tissue specificity of expression of heat shock gene ATHSP70-10 in Arabidopsis thaliana seedlings under normal and stress conditions

2020 ◽  
Vol 2020 (3) ◽  
pp. 37-47
Author(s):  
L.Ye. Kozeko ◽  
◽  
E.L. Kordyum ◽  
Keyword(s):  
Arabidopsis Thaliana ◽  
Heat Shock ◽  
Water Deficit ◽  
Tissue Specificity ◽  
Abiotic Factors ◽  
Root Apex ◽  
Stress Conditions ◽  
Heat Shock Gene ◽  
Organ Specificity ◽  
Pcr Analysis

Mitochondrial heat shock proteins of HSP70 family support protein homeostasis in mitochondria under normal and stress conditions. They provide folding and complex assembly of proteins encoded by mitochondrial genome, as well as import of cytosolic proteins to mitochondria, their folding and protection against aggregation. There are reports about organ-specificity of mitochondrial HSP70 synthesis in plants. However, tissue specificity of their functioning remains incompletely characterized. This problem was studied for mitochondrial AtHSP70-10 in Arabidopsis thaliana seedlings using a transgenic line with uidA signal gene under normal conditions, as well as high temperature and water deficit. Under normal conditions, histochemical GUS-staining revealed the expression of AtHSP70-10 in cotyledon and leaf hydathodes, stipules, central cylinder in root differentiation and mature zones, as well as weak staining in root apex and root-shoot junction zone. RT-PCR analysis of wild-type seedlings exposed to 37°C showed rapid upregulation of AtHSP70-10, which reached the highest level within 2 h. In addition, the gradual development of water deficit for 5 days caused an increase in transcription of this gene, which became more pronounced after 3 days and reached a maximum after 5 days of dehydration. Histochemical analysis showed complete preservation of tissue localization of AtHSP70-10 expression under both abiotic factors. The data obtained indicate the specific functioning of mitochondrial chaperone AtHSP70-10 in certain plant cellular structures.

Photosynthetic and anatomical responses of three plant species at two altitudinal levels in the Neotropical savannah

2016 ◽  
Vol 64 (8) ◽  
pp. 696 ◽  
Author(s):  
Vinícius Coelho Kuster ◽  
Silvana Aparecida Barbosa de Castro ◽  
Fernando Henrique Aguiar Vale
Keyword(s):  
Quantum Yield ◽  
Photosystem Ii ◽  
Plant Species ◽  
Photosynthetic Pigments ◽  
Abiotic Factors ◽  
Palisade Parenchyma ◽  
Structural Modifications ◽  
Physiological Alterations ◽  
Roupala Montana ◽  
Plastic Responses

The phytophysiognomies of the Neotropical savannah occur at different altitudes, which can determine distinctions in the levels of light and shade that plants are exposed. The focus of the study is analysing the functional traits of the leaves of Byrsonima verbascifolia (L.) Rich., Roupala montana Aubl. and Solanum lycocarpum A. St.-Hil. growing in phytophysiognomies at two distinct altitudes. We evaluated leaf anatomy, the quantum yield of photosystem II, and the photosynthetic pigments in plants occurring at two areas of Campo sujo, which are separated by 700 m of altitude, during the rainy season. The three plant species occurring at higher altitudes had thicker adaxial epidermis. B. verbascifolia and S. lycocarpum occurring at higher altitudes had thicker palisade parenchyma, whereas only B. verbascifolia had thicker spongy parenchyma at the same site. The quantum yield of photosystem II, and photosynthetic pigments had little differences between plants occurring at higher and lower altitudes. The results analysed show high structural modifications and low physiological alterations from altitudinal conditions. Thus, the influence of the abiotic factors appears to modulate the plastic responses of plants across altitude.

scholarly journals ASSESSMENT OF THE INFLUENCE OF ABIOTIC FACTORS ON THE ENZYMATIVE ACTIVITY OF THE SOIL

ÈKOBIOTEH ◽  
2021 ◽  
Vol 4 (2) ◽  
pp. 128-134
Author(s):  
E.V. Tovstik ◽  
◽  
A.S. Olkova ◽  
Keyword(s):  
Enzymatic Activity ◽  
Soil Solution ◽  
Ph Value ◽  
Abiotic Factors ◽  
Inverse Correlation ◽  
Invertase Activity ◽  
Soil Environment ◽  
Factors Affecting ◽  
Activity Of Enzymes ◽  
Made In

Аn attempt is made in this work to establish correlations between the level of enzymatic activity of the soil and factors of an abiotic nature. It was found that the activity of invertase and urease in the soils of more southern territories is higher than that of northern ones. In soils with a pH value of the soil environment close to neutral reaction, the level of enzymatic activity is higher than in more acidic ones. The most sensitive to soil acidity among the studied urease enzymes. In relation to zinc, an inverse correlation was established between its content in the soil and the level of invertase activity. According to the degree of resistance to salinity, the enzymes are arranged in the following order: catalase> invertase> urease. An increase in the mineralization of the soil solution leads to an increase in the activity of urease. Of the studied enzymes, the most labile are representatives of the class of hydrolases (invertase and urease), less labile are oxidoreductases (catalase). Thus, when diagnosing the state of the soil by the level of enzymatic activity, it is necessary to take into account the main abiotic factors affecting the activity of enzymes: the average annual air temperature; pH and mineralization of the soil solution; the content of substances that inhibit microorganisms and block exozymes.

scholarly journals Effects of cadmium-induced oxidative stress on growth and nitrogen assimilation in blackgram [Vigna mungo (L.) Hepper]

2013 ◽  
Vol 58 (1) ◽  
pp. 31-39
Author(s):  
Mohammad Mobin
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Nitrogen Assimilation ◽  
Dry Weight ◽  
Vigna Mungo ◽  
Cd Stress ◽  
Cd Accumulation ◽  
Shoot Dry Weight ◽  
Activity Of Enzymes ◽  
Consequent Increase

Cadmium (Cd) accumulation, oxidative damage, and nitrogen metabolism were studied in roots and leaves of 30-d-old blackgram plants [Vigna mungo (L.) Hepper], grown in a mixture of soil and compost (3:1) with different Cd concentrations. Significant reductions in both root and shoot dry weight were noted. The concentration of Cd in roots and leaves increased with increasing Cd levels. The level of lipid peroxidation elevated with a consequent increase in H2O2 content under Cd stress in both plant organs. The activity of enzymes mediating the nitrogen assimilation in roots and leaves was greatly reduced in the presence of Cd, except glutamate dehydrogenase (GDH) which showed a significant increase.

scholarly journals Co-inoculation with Azospirillum brasilense in soybean cultivars subjected to water déficit

2020 ◽  
Vol 24 (2) ◽  
pp. 89-94 ◽  
Author(s):  
Alessandra M. de L. Naoe ◽  
Joênes M. Peluzio ◽  
Leonardo J. M. Campos ◽  
Lucas K. Naoe ◽  
Roberta A. e Silva
Keyword(s):  
Grain Yield ◽  
Water Deficit ◽  
Bradyrhizobium Japonicum ◽  
Azospirillum Brasilense ◽  
Field Experiments ◽  
Crop Evapotranspiration ◽  
Plant Genotype ◽  
Soybean Cultivars ◽  
Sowing Dates ◽  
Soybean Plants

ABSTRACT This study aimed to verify the effect of co-inoculation, association between Azospirillum brasilense and Bradyrhizobium japonicum bacteria, on soybean plants subjected to water deficit at two sowing dates. Two field experiments were conducted at the Universidade Federal de Tocantins, campus of Palmas, Brazil, in 2016. The experimental design was randomized blocks in a split-split-plot arrangement with four repetitions, where the plots consisted of two irrigation depths (100 and 25% of crop evapotranspiration - ETc), the subplots was composed of two methods of inoculant application (inoculation with Bradyrhizobium japonicum and co-inoculation with Azospirillum brasilense + Bradyrhizobium japonicum) and the sub-subplots comprised two soybean cultivars (TMG 132 and ANTA 82). The cultivars responded differently to the sowing dates. Co-inoculation did not influence grain yield under full irrigation conditions (100% ETc), in neither cultivar evaluated. However, under the water deficit condition (25% ETc), the grain yield of the cultivar TMG 132 increased 77.20%, indicating that there are different responses of interaction between Azospirillum brasilense, plant genotype and sowing dates.

scholarly journals Susceptibility to weathering damage and oxidative stress on soybean seeds with different lignin contents in the seed coat

2016 ◽  
Vol 38 (4) ◽  
pp. 296-304 ◽  
Author(s):  
Caroline Huth ◽  
Liliane Marcia Mertz-Henning ◽  
Sidnei Jose Lopes ◽  
Luciane Almeri Tabaldi ◽  
Liana Veronica Rossato ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Seed Coat ◽  
Lignin Content ◽  
Accelerated Aging ◽  
Guaiacol Peroxidase ◽  
Soybean Seeds ◽  
Soybean Cultivars ◽  
Activity Of Enzymes ◽  
The Relationship ◽  
And Oxidative Stress

Abstract: This study aimed at evaluating the relationship among lignin content in the seed coat of soybean seeds with tolerance to weathering damage and oxidative stress. Four soybean cultivars (CD 208, CD 215, CD 202 and BRS 184), with lignin contents in the seed coat of 4.2, 2.6, 2.0 and 1.7%, respectively, were used. Seeds were subjected to deterioration by moisture, through artificially simulated rains. Pods of all cultivars were subjected to irrigation by constant sprinkling during 4 hours, and then dried in oven, set at 35 °C, for 37 hours; repeating twice such procedure. After these treatments seeds were subjected to tests and then compared to the control. Tests performed were: germination, accelerated aging and tetrazolium test; thereby quantifying the occurrence of damages by weathering damage. The activity of enzymes superoxide dismutase and guaiacol peroxidase was measured; and the lipid peroxidation was assessed. Soybean cultivars CD 208 and CD 215, with higher lignin content in the seed coat, were less susceptible to weathering damage. Seeds of cultivar CD 208 have also presented lower oxidative stress, when subjected to weathering damage stress.

scholarly journals TOLERANCE OF F 2 POPULATIONS OF COWPEA TO WATER DEFICIT

2018 ◽  
Vol 31 (1) ◽  
pp. 48-55
Author(s):  
ERINA VITÓRIO RODRIGUES ◽  
KAESEL JACKSON DAMASCENO-SILVA ◽  
MAURISRAEL DE MOURA ROCHA ◽  
EDSON ALVES BASTOS ◽  
ADRIANO DOS SANTOS
Keyword(s):  
Water Deficit ◽  
Development Stage ◽  
Incomplete Block ◽  
Grain Production ◽  
Full Irrigation ◽  
Average Decrease ◽  
Relative Production ◽  
High Production ◽  
Experimental Plots ◽  
Block Experiment

ABSTRACT Water deficit affects plant development and the overcoming of its effects depends on the genotype, duration of stress, severity of damage and development stage of the plant at water stress. Cowpea is considered tolerant to water deficit, however, studies have shown that its yield can be reduced under this condition. Thus, the objective of this work was to evaluate the effect of water deficit on F2 populations of cowpea and select tolerant genotypes. Thirty F2 populations of cowpea and their parents (BRS-Paraguaçu, Pingo-de-Ouro-1-2, BRS-Xiquexique, CNCx-698-128G, Santo-Inácio and MNC99-510F-16-1) were evaluated in two experiments, one under induced water deficit and other under full irrigation, in the experimental field of the Embrapa Mid-North, in Teresina, State of Piauí, Brazil. A triple lattice incomplete block experiment design was used, with three replications. The experimental plots consisted of six 2-m rows, with spacing of 1.0 m between rows and 0.50 m between plants. The grain production was evaluated and its decrease related to the relative production and tolerance to stress was calculated. The grain production of the genotypes had an average decrease of 29.83% under water deficit. The genotypes from the crosses BRS-Paraguaçu x CNCx-698-128G, BRS-Xiquexique x Pingo-de-Ouro-1-2, CNCx-698-128G x BRS-Xiquexique, CNCx-698-128G x MNC99-510F-16-1, Santo-Inácio x BRS-Xiquexique and MNC99-510F-16-1 x BRS-Paraguaçu had high production and high tolerance to water deficit.

scholarly journals SUGARCANE CROPS WITH CONTROLLED WATER DEFICIT IN THE SUB-MIDDLE SÃO FRANCISCO VALLEY, BRAZIL

2018 ◽  
Vol 31 (4) ◽  
pp. 963-971 ◽  
Author(s):  
WELSON LIMA SIMÕES ◽  
MARCELO CALGARO ◽  
MIGUEL JULIO MACHADO GUIMARÃES ◽  
ANDERSON RAMOS DE OLIVEIRA ◽  
MÍRIAN PAULA MEDEIROS ANDRÉ PINHEIRO
Keyword(s):  
Water Deficit ◽  
Block Design ◽  
Development Stage ◽  
Maturation Stage ◽  
Randomized Block Design ◽  
Sugarcane Yield ◽  
Maturation Stages ◽  
Crop Cycle ◽  
Technological Quality

ABSTRACT Sugarcane is one of the most affected crops by water scarcity. The efficient use of the irrigation water is an alternative to minimize this problem. The objective of this work was to evaluate biometric parameters, yield, and technological quality of sugarcane plants subjected to different controlled water deficit regimes in the sub-middle São Francisco Valley, Brazil. The experiment was conducted in a randomized block design, in two crop cycles, with three replications, with ten treatments consisted of three controlled water deficits (15%, 30%, and 45% of the crop evapotranspiration - ETc), applied at three development stages of the plant - sprouting and tillering (Stage I), grand growth (Stage II), and maturation (Stage III) - and a control with 100% of the ETc throughout the entire crop cycle. The controlled water deficit did not affect the technological quality of the sugarcane in any development stage. The sugarcane yield was higher when using a controlled water deficit of 30% of ETc in the sprouting and tillering stages of the plants. The water deficit of 15% of ETc is recommended for the grand growth, or maturation stages of the sugarcane plants for a greater water use efficiency of the production system.

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